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  • 76
    4Gene polymerase chain reaction polymerase chain reaction pcr amplification
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Polymerase Chain Reaction Polymerase Chain Reaction Pcr Amplification, supplied by 4Gene, used in various techniques. Bioz Stars score: 76/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 76 stars, based on 4 article reviews
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    99
    Thermo Fisher polymerase chain reactions pcr
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reactions Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 701 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 701 article reviews
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    polymerase chain reactions pcr - by Bioz Stars, 2020-02
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    78
    ReaMetrix pcr polymerase chain reaction
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Pcr Polymerase Chain Reaction, supplied by ReaMetrix, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    tiangen biotech co polymerase chain reactions pcr
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reactions Pcr, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 88/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    TransGen biotech co polymerase chain reactions pcr
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reactions Pcr, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 19 article reviews
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    Agilent technologies polymerase chain reaction pcr tubes
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr Tubes, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies quikchange polymerase chain reaction pcr
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Quikchange Polymerase Chain Reaction Pcr, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Axygen polymerase chain reaction pcr tubes
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr Tubes, supplied by Axygen, used in various techniques. Bioz Stars score: 84/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polymerase chain reaction pcr amplification
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr Amplification, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polymerase chain reaction pcr machine
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr Machine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioneer Corporation polymerase chain reaction pcr premix
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr Premix, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 90/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    GE Healthcare polymerase chain reaction pcr buffer
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche polymerase chain reaction pcr assay
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr Assay, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shanghai Generay Biotech polymerase chain reaction pcr primers
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr Primers, supplied by Shanghai Generay Biotech, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    TaKaRa polymerase chain reaction pcr machine
    <t>PCR</t> amplified object fragment. The agarose gel electrophoresis revealed that the object band was in the expected position. M, DL2000 DNA marker; 1 and 2, PCR product (502 bp) <t>cDNA.</t> PCR, polymerase chain reaction.
    Polymerase Chain Reaction Pcr Machine, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eurofins polymerase chain reaction pcr primers
    <t>PCR</t> amplified object fragment. The agarose gel electrophoresis revealed that the object band was in the expected position. M, DL2000 DNA marker; 1 and 2, PCR product (502 bp) <t>cDNA.</t> PCR, polymerase chain reaction.
    Polymerase Chain Reaction Pcr Primers, supplied by Eurofins, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Millipore polymerase chain reaction pcr assay
    <t>PCR</t> amplified object fragment. The agarose gel electrophoresis revealed that the object band was in the expected position. M, DL2000 DNA marker; 1 and 2, PCR product (502 bp) <t>cDNA.</t> PCR, polymerase chain reaction.
    Polymerase Chain Reaction Pcr Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polymerase chain reaction pcr primers
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    Image Search Results


    Agarose gel (2%) showing PCR amplification of TLR-4 (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele

    Journal: Indian Journal of Urology : IJU : Journal of the Urological Society of India

    Article Title: Asp299Gly and Thr399Ile polymorphism of TLR-4 gene in patients with prostate cancer from North India

    doi: 10.4103/0970-1591.109982

    Figure Lengend Snippet: Agarose gel (2%) showing PCR amplification of TLR-4 (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele

    Article Snippet: Polymerase chain reaction Polymerase chain reaction (PCR) amplification of TLR-4 gene fragments was performed in Perkin Elmer thermocycler (USA) using gene-specific primers [ ].

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Variant Assay

    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by qRT-PCR. Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p

    Journal: Stem Cells (Dayton, Ohio)

    Article Title: Calcium and Calcineurin-NFAT Signaling Regulate Granulocyte-Monocyte Progenitor Cell Cycle via Flt3-L

    doi: 10.1002/stem.1813

    Figure Lengend Snippet: Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by qRT-PCR. Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p

    Article Snippet: Reverse transcription was carried out using high-capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems), or with SuperScript III First Strand Synthesis System for RT-polymerase chain reaction (PCR) (Invitrogen).

    Techniques: Inhibition, In Vitro, Expressing, Cell Culture, Mouse Assay, Quantitative RT-PCR

    PCR amplified object fragment. The agarose gel electrophoresis revealed that the object band was in the expected position. M, DL2000 DNA marker; 1 and 2, PCR product (502 bp) cDNA. PCR, polymerase chain reaction.

    Journal: Molecular Medicine Reports

    Article Title: Lentivirus transduced interleukin-1 receptor antagonist gene expression in murine bone marrow-derived mesenchymal stem cells in vitro

    doi: 10.3892/mmr.2015.4003

    Figure Lengend Snippet: PCR amplified object fragment. The agarose gel electrophoresis revealed that the object band was in the expected position. M, DL2000 DNA marker; 1 and 2, PCR product (502 bp) cDNA. PCR, polymerase chain reaction.

    Article Snippet: The IL-1Ra cDNA was amplified using a polymerase chain reaction (PCR) machine (Takara Bio, Inc.) and the expression vector was cloned and validated by sequencing (Invitrogen Life Technologies).

    Techniques: Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Marker