pcr reaction Search Results


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  • 99
    Thermo Fisher polymerase chain reaction pcr reactions
    Polymerase Chain Reaction Pcr Reactions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    4Gene polymerase chain reaction polymerase chain reaction pcr amplification
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Polymerase Chain Reaction Polymerase Chain Reaction Pcr Amplification, supplied by 4Gene, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1468 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ReaMetrix pcr polymerase chain reaction
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Pcr Polymerase Chain Reaction, supplied by ReaMetrix, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co polymerase chain reaction pcr
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 95/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TransGen biotech co polymerase chain reaction pcr
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega polymerase chain reaction kit
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction primers
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioMed Diagnostics Inc polymerase chain reaction processor
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Processor, supplied by BioMed Diagnostics Inc, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Jackson Laboratory polymerase chain reaction protocol
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Protocol, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega transcription polymerase chain reaction
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Transcription Polymerase Chain Reaction, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction amplification
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Amplification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PAN - Biotech regular polymerase chain reaction
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Regular Polymerase Chain Reaction, supplied by PAN - Biotech, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche tb polymerase chain reaction
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Tb Polymerase Chain Reaction, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioTechniques polymerase chain reaction biotechniques
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Biotechniques, supplied by BioTechniques, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polymerase chain reaction gradient
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Gradient, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Greiner Bio polymerase chain reaction tubes
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Tubes, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Luminex polymerase chain reaction typing
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Typing, supplied by Luminex, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman polymerase chain reaction
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Taqman Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher transcription polymerase chain reaction
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Transcription Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 571 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SensoQuest polymerase chain reaction pcr application
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Pcr Application, supplied by SensoQuest, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Medicina polymerase chain reaction pcr medicina
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Pcr Medicina, supplied by Medicina, used in various techniques. Bioz Stars score: 84/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioneer Corporation polymerase chain reaction pcr premix
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
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    Bioneer Corporation polymerase chain reaction pcr premixes
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
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    Operon Biotech polymerase chain reaction pcr primers
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Pcr Primers, supplied by Operon Biotech, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sangon Biotech polymerase chain reaction pcr primers
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Pcr Primers, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 93/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Axygen polymerase chain reaction pcr tubes
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Pcr Tubes, supplied by Axygen, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioneer Corporation polymerase chain reaction primers
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Primers, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MIDSCI polymerase chain reaction vials
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Vials, supplied by MIDSCI, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction polymerase chain reaction pcr
    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
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    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
    Polymerase Chain Reaction Pcr, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Agarose gel (2%) showing PCR amplification of TLR-4 (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele

    Journal: Indian Journal of Urology : IJU : Journal of the Urological Society of India

    Article Title: Asp299Gly and Thr399Ile polymorphism of TLR-4 gene in patients with prostate cancer from North India

    doi: 10.4103/0970-1591.109982

    Figure Lengend Snippet: Agarose gel (2%) showing PCR amplification of TLR-4 (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele

    Article Snippet: Polymerase chain reaction Polymerase chain reaction (PCR) amplification of TLR-4 gene fragments was performed in Perkin Elmer thermocycler (USA) using gene-specific primers [ ].

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Variant Assay

    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by qRT-PCR. Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p

    Journal: Stem Cells (Dayton, Ohio)

    Article Title: Calcium and Calcineurin-NFAT Signaling Regulate Granulocyte-Monocyte Progenitor Cell Cycle via Flt3-L

    doi: 10.1002/stem.1813

    Figure Lengend Snippet: Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by qRT-PCR. Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p

    Article Snippet: Reverse transcription was carried out using high-capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems), or with SuperScript III First Strand Synthesis System for RT-polymerase chain reaction (PCR) (Invitrogen).

    Techniques: Inhibition, In Vitro, Expressing, Cell Culture, Mouse Assay, Quantitative RT-PCR

    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time RT-PCR for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with U6. Cord blood (CB) and peripheral blood (PB)

    Journal: Blood

    Article Title: Cell-cycle regulator E2F1 and microRNA-223 comprise an autoregulatory negative feedback loop in acute myeloid leukemia

    doi: 10.1182/blood-2009-08-240101

    Figure Lengend Snippet: miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time RT-PCR for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with U6. Cord blood (CB) and peripheral blood (PB)

    Article Snippet: Corresponding reverse-transcription (RT) and polymerase chain reaction (PCR) primers for miRNA-223 and U6 were obtained from Applied Biosystems.

    Techniques: Quantitative RT-PCR, Derivative Assay