pcr reaction Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher pcr reaction
    Pcr Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr reaction/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr reaction - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    86
    Bio-Rad pcr reactions
    Expression of E1 during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad <t>QX200</t> droplet digital <t>PCR</t> system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: E1A ≤ 0.001; E1B-19k
    Pcr Reactions, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr reactions/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr reactions - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    TaKaRa pcr reaction
    (A) Nonsense mutation in gibbon SIRH11/ZCCHC16 . The four bp deletion (blue in a red box) in gibbon leads to a nonsense mutation (red). Note that there is a G to A transition <t>(DNA</t> polymorphism) in a stop codon of gibbon (TAA) compared with human/chimpanzee and other primates (TAG). (B) <t>PCR</t> analysis of gibbon SIRH11/ZCCHC16 . Upper panel shows the schematic representation of primer design. Lower panel shows agarose gel electrophoresis profile in each primer set. The arrows represent expected band size. M, 100 bp and 1 kb ladder; Gor, gorilla; Hla, white-handed gibbon; Ssy, siamang; Rhe, rhesus macaque; Mar, common marmoset; Sol, solvent only (no DNA). (C) Southern blot analysis of Hla and Ssy . Left and right panels show the result of hybridization using SIRH11 and TYR probes, respectively. The arrows indicate expected band size. Gor, gorilla; Hla, white-handed gibbon; Ssy, siamang; Rhe, rhesus macaque; Mar, common marmoset. (D) Amino acid sequence alignment of Chiroptera SIRH11/ZCCHC16. The blue asterisks in a red box indicate a common nonsense mutation in megachiroptera. The asterisks, colons and periods below the amino acids indicate identical, strongly and weakly similar residues among six species, respectively. (E) DNA alignments around the common TAA nonsense mutation (red).
    Pcr Reaction, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr reaction/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr reaction - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Bio-Rad pcr reaction
    Agarose gel electrophoresis of <t>DNA</t> amplified from various genomic DNA extracts of Korean native honey (KNH) and European honey (EH) in duplex <t>PCR</t> with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M2-F and M2-R.
    Pcr Reaction, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr reaction/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr reaction - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier


    N/A
    Cole Parmer PCR products are specifically designed for amplifying nucleic acids during the Polymerase Chain Reaction PCR process Certified RNase DNase and pyrogen safe to ensure no degradation of the
      Buy from Supplier

    Image Search Results


    Expression of E1 during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: E1A ≤ 0.001; E1B-19k

    Journal: PLoS ONE

    Article Title: Temporal dynamics of adenovirus 5 gene expression in normal human cells

    doi: 10.1371/journal.pone.0211192

    Figure Lengend Snippet: Expression of E1 during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: E1A ≤ 0.001; E1B-19k

    Article Snippet: PCR reactions were carried out using EvaGreen for QX200 master mix according to manufacturer’s directions using a QX200 digital droplet PCR instrument (BioRad).

    Techniques: Expressing, Infection, Digital PCR, Real-time Polymerase Chain Reaction, Standard Deviation

    Expression of E2 , E3 , and E4 during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at an MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: DBP

    Journal: PLoS ONE

    Article Title: Temporal dynamics of adenovirus 5 gene expression in normal human cells

    doi: 10.1371/journal.pone.0211192

    Figure Lengend Snippet: Expression of E2 , E3 , and E4 during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at an MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: DBP

    Article Snippet: PCR reactions were carried out using EvaGreen for QX200 master mix according to manufacturer’s directions using a QX200 digital droplet PCR instrument (BioRad).

    Techniques: Expressing, Infection, Digital PCR, Real-time Polymerase Chain Reaction, Standard Deviation

    Expression of VAII RNA during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the p ≤ 0.0027. All time-points between 17 and 36 hours were statistically significant.

    Journal: PLoS ONE

    Article Title: Temporal dynamics of adenovirus 5 gene expression in normal human cells

    doi: 10.1371/journal.pone.0211192

    Figure Lengend Snippet: Expression of VAII RNA during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the p ≤ 0.0027. All time-points between 17 and 36 hours were statistically significant.

    Article Snippet: PCR reactions were carried out using EvaGreen for QX200 master mix according to manufacturer’s directions using a QX200 digital droplet PCR instrument (BioRad).

    Techniques: Expressing, Infection, Digital PCR, Real-time Polymerase Chain Reaction, Standard Deviation

    Analysis of viral genome copy number in the first 48 hours of infection with HAdV5. (A) Arrested IMR-90 cells were infected with dl 309 at a MOI of 50. Viral DNA was extracted at the indicated time points, and absolute quantification of viral genomes using E4orf3 primers was measured using the BioRad QX200 droplet digital PCR system. Viral genomes are plotted on per cell basis. Error bars represent standard deviation of biological replicates, n = 2. Bar with asterisk represents changes above mock level that are statistically significant with p ≤ 0.0001. (B) Sample droplet distribution from one of the biological replicates for 29 and 30 hour time points from (A).

    Journal: PLoS ONE

    Article Title: Temporal dynamics of adenovirus 5 gene expression in normal human cells

    doi: 10.1371/journal.pone.0211192

    Figure Lengend Snippet: Analysis of viral genome copy number in the first 48 hours of infection with HAdV5. (A) Arrested IMR-90 cells were infected with dl 309 at a MOI of 50. Viral DNA was extracted at the indicated time points, and absolute quantification of viral genomes using E4orf3 primers was measured using the BioRad QX200 droplet digital PCR system. Viral genomes are plotted on per cell basis. Error bars represent standard deviation of biological replicates, n = 2. Bar with asterisk represents changes above mock level that are statistically significant with p ≤ 0.0001. (B) Sample droplet distribution from one of the biological replicates for 29 and 30 hour time points from (A).

    Article Snippet: PCR reactions were carried out using EvaGreen for QX200 master mix according to manufacturer’s directions using a QX200 digital droplet PCR instrument (BioRad).

    Techniques: Infection, Digital PCR, Standard Deviation

    Expression of viral late genes during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: 52k EP ≤ 0.001; pIIIa ≤ 0.0006; Penton base ≤ 0.0001; Hexon ≤ 0.0002; 100k HAP ≤ 0.0007; Fiber ≤ 0.0004. All time-points between 17 and 36 hours were statistically significant.

    Journal: PLoS ONE

    Article Title: Temporal dynamics of adenovirus 5 gene expression in normal human cells

    doi: 10.1371/journal.pone.0211192

    Figure Lengend Snippet: Expression of viral late genes during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: 52k EP ≤ 0.001; pIIIa ≤ 0.0006; Penton base ≤ 0.0001; Hexon ≤ 0.0002; 100k HAP ≤ 0.0007; Fiber ≤ 0.0004. All time-points between 17 and 36 hours were statistically significant.

    Article Snippet: PCR reactions were carried out using EvaGreen for QX200 master mix according to manufacturer’s directions using a QX200 digital droplet PCR instrument (BioRad).

    Techniques: Expressing, Infection, Digital PCR, Real-time Polymerase Chain Reaction, Standard Deviation

    (A) Nonsense mutation in gibbon SIRH11/ZCCHC16 . The four bp deletion (blue in a red box) in gibbon leads to a nonsense mutation (red). Note that there is a G to A transition (DNA polymorphism) in a stop codon of gibbon (TAA) compared with human/chimpanzee and other primates (TAG). (B) PCR analysis of gibbon SIRH11/ZCCHC16 . Upper panel shows the schematic representation of primer design. Lower panel shows agarose gel electrophoresis profile in each primer set. The arrows represent expected band size. M, 100 bp and 1 kb ladder; Gor, gorilla; Hla, white-handed gibbon; Ssy, siamang; Rhe, rhesus macaque; Mar, common marmoset; Sol, solvent only (no DNA). (C) Southern blot analysis of Hla and Ssy . Left and right panels show the result of hybridization using SIRH11 and TYR probes, respectively. The arrows indicate expected band size. Gor, gorilla; Hla, white-handed gibbon; Ssy, siamang; Rhe, rhesus macaque; Mar, common marmoset. (D) Amino acid sequence alignment of Chiroptera SIRH11/ZCCHC16. The blue asterisks in a red box indicate a common nonsense mutation in megachiroptera. The asterisks, colons and periods below the amino acids indicate identical, strongly and weakly similar residues among six species, respectively. (E) DNA alignments around the common TAA nonsense mutation (red).

    Journal: Frontiers in Chemistry

    Article Title: An LTR Retrotransposon-Derived Gene Displays Lineage-Specific Structural and Putative Species-Specific Functional Variations in Eutherians

    doi: 10.3389/fchem.2016.00026

    Figure Lengend Snippet: (A) Nonsense mutation in gibbon SIRH11/ZCCHC16 . The four bp deletion (blue in a red box) in gibbon leads to a nonsense mutation (red). Note that there is a G to A transition (DNA polymorphism) in a stop codon of gibbon (TAA) compared with human/chimpanzee and other primates (TAG). (B) PCR analysis of gibbon SIRH11/ZCCHC16 . Upper panel shows the schematic representation of primer design. Lower panel shows agarose gel electrophoresis profile in each primer set. The arrows represent expected band size. M, 100 bp and 1 kb ladder; Gor, gorilla; Hla, white-handed gibbon; Ssy, siamang; Rhe, rhesus macaque; Mar, common marmoset; Sol, solvent only (no DNA). (C) Southern blot analysis of Hla and Ssy . Left and right panels show the result of hybridization using SIRH11 and TYR probes, respectively. The arrows indicate expected band size. Gor, gorilla; Hla, white-handed gibbon; Ssy, siamang; Rhe, rhesus macaque; Mar, common marmoset. (D) Amino acid sequence alignment of Chiroptera SIRH11/ZCCHC16. The blue asterisks in a red box indicate a common nonsense mutation in megachiroptera. The asterisks, colons and periods below the amino acids indicate identical, strongly and weakly similar residues among six species, respectively. (E) DNA alignments around the common TAA nonsense mutation (red).

    Article Snippet: The PCR reaction was performed using PrimeSTAR GXL DNA Polymerase (TaKaRa, Japan) with the following conditions: 94°C, 2 min; 36 cycles of 98°C, 10 s; 55°C, 15 s; 68°C, 20 s; final extention 68°C, 60 s. The following PCR primers were used: Gorilla_SIRH11_F1: 5′-GAGGGAGGAGAGAAAGGTACTG-3′ and Gorilla_SIRH11_R1: 5′-TCTGAGCAATTGGCAGGGTC-3′.

    Techniques: Mutagenesis, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Southern Blot, Hybridization, Sequencing

    (A) Amino acid sequence alignment of Platyrrhihi SIRH11/ZCCHC16 . The blue asterisks indicate common nonsense mutations in Platyrrhihi. The underlined sequences indicate the putative short ORFs starting from a next Met codon. The asterisks, colons and periods below the amino acids indicate identical, strongly and weakly similar residues among three species, respectively. (B) PCR analysis of Platyrrhihi SIRH11/ZCCHC16 . Upper panel shows the schematic representation of the primer design. Lower panel shows agarose gel electrophoresis profile in each primer set. The arrows represent expected band size. M, 100 bp and 1 kb ladder; Hum, human; Rhe, rhesus macaque; Cap, tufted capuchin; Mar, common marmoset; Owl, the Azara's owl monkey; Spi, long-haired spider monkey; Squ, common squirrel monkey; Tam, cotton-top tamarin; Sol, solvent only (no DNA). (C) DNA sequence analysis of Platyrrhihi SIRH11/ZCCHC16 . DNA sequences of Azara's owl monkey, common marmoset, tufted capuchin, and long-haired spider monkey determined by our own experiments are also shown. Magenta boxes show lineage specific insertion or deletion. The underlined letters indicate the SIRH11/ZCCHC16 ORF. (D) Amino acid sequence alignment of Hystricognathi SIRH11/ZCCHC16. The similar sequences among five Hystricognathi species are expressed in green. The red asterisks and Xs indicate the sites of ORF termination and frameshift, respectively. The blue asterisks in a red box indicate a common nonsense mutation in Hystricognathi. The underlined sequences indicate the putative short ORFs starting from a next Met codon. The asterisks, colons and periods below the amino acids indicate identical, strongly and weakly similar residues among six species, respectively. The house mouse sequence is used as a reference. (E) Amino acid sequence alignment of Cetartiodactyla SIRH11/ZCCHC16. The blue asterisks in red boxes indicate common nonsense mutations in Cetartiodactyla. The red asterisks and Xs indicate the sites of ORF termination and frameshift, respectively. The underlined sequences indicate the putative short ORFs starting from a next Met codon. The similar sequences among 14 Cetartiodactyla species are shown in green. The asterisks, colons and periods below the amino acids indicate identical, strongly and weakly similar residues among 14 species, respectively. The pig sequence is used as a reference. (F) Sequence analysis of gorilla SIRH11/ZCCHC16 . Upper panel shows the schematic representation of the primer design and nonsense mutation site (red asterisk) in gorilla SIRH11/ZCCHC16 . Middle panel shows the sequence comparison between gorilla, human, and chimpanzee. Lower panel represents the sequence results of three individuals.

    Journal: Frontiers in Chemistry

    Article Title: An LTR Retrotransposon-Derived Gene Displays Lineage-Specific Structural and Putative Species-Specific Functional Variations in Eutherians

    doi: 10.3389/fchem.2016.00026

    Figure Lengend Snippet: (A) Amino acid sequence alignment of Platyrrhihi SIRH11/ZCCHC16 . The blue asterisks indicate common nonsense mutations in Platyrrhihi. The underlined sequences indicate the putative short ORFs starting from a next Met codon. The asterisks, colons and periods below the amino acids indicate identical, strongly and weakly similar residues among three species, respectively. (B) PCR analysis of Platyrrhihi SIRH11/ZCCHC16 . Upper panel shows the schematic representation of the primer design. Lower panel shows agarose gel electrophoresis profile in each primer set. The arrows represent expected band size. M, 100 bp and 1 kb ladder; Hum, human; Rhe, rhesus macaque; Cap, tufted capuchin; Mar, common marmoset; Owl, the Azara's owl monkey; Spi, long-haired spider monkey; Squ, common squirrel monkey; Tam, cotton-top tamarin; Sol, solvent only (no DNA). (C) DNA sequence analysis of Platyrrhihi SIRH11/ZCCHC16 . DNA sequences of Azara's owl monkey, common marmoset, tufted capuchin, and long-haired spider monkey determined by our own experiments are also shown. Magenta boxes show lineage specific insertion or deletion. The underlined letters indicate the SIRH11/ZCCHC16 ORF. (D) Amino acid sequence alignment of Hystricognathi SIRH11/ZCCHC16. The similar sequences among five Hystricognathi species are expressed in green. The red asterisks and Xs indicate the sites of ORF termination and frameshift, respectively. The blue asterisks in a red box indicate a common nonsense mutation in Hystricognathi. The underlined sequences indicate the putative short ORFs starting from a next Met codon. The asterisks, colons and periods below the amino acids indicate identical, strongly and weakly similar residues among six species, respectively. The house mouse sequence is used as a reference. (E) Amino acid sequence alignment of Cetartiodactyla SIRH11/ZCCHC16. The blue asterisks in red boxes indicate common nonsense mutations in Cetartiodactyla. The red asterisks and Xs indicate the sites of ORF termination and frameshift, respectively. The underlined sequences indicate the putative short ORFs starting from a next Met codon. The similar sequences among 14 Cetartiodactyla species are shown in green. The asterisks, colons and periods below the amino acids indicate identical, strongly and weakly similar residues among 14 species, respectively. The pig sequence is used as a reference. (F) Sequence analysis of gorilla SIRH11/ZCCHC16 . Upper panel shows the schematic representation of the primer design and nonsense mutation site (red asterisk) in gorilla SIRH11/ZCCHC16 . Middle panel shows the sequence comparison between gorilla, human, and chimpanzee. Lower panel represents the sequence results of three individuals.

    Article Snippet: The PCR reaction was performed using PrimeSTAR GXL DNA Polymerase (TaKaRa, Japan) with the following conditions: 94°C, 2 min; 36 cycles of 98°C, 10 s; 55°C, 15 s; 68°C, 20 s; final extention 68°C, 60 s. The following PCR primers were used: Gorilla_SIRH11_F1: 5′-GAGGGAGGAGAGAAAGGTACTG-3′ and Gorilla_SIRH11_R1: 5′-TCTGAGCAATTGGCAGGGTC-3′.

    Techniques: Sequencing, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Mutagenesis

    (A) Common nonsense mutation among xenarthra species. Multiple sequence alignment was constructed using five amino acid sequences; armadillo1, Dasypus novemcinctus ; armadillo2, Tolypeutes matacus ; sloth1, Choloepus hoffmanni ; sloth2, Choloepus didactylus and manatee, Florida manatee as a reference. The red asterisks and Xs show the sites of ORF termination and frameshift, respectively. The blue asterisks in a red box indicate a common mutation among three species. Purple characters indicate CCHC amino acids in the RNA binding domain. (B) DNA alignments around the common TAG nonsense mutation (red) indicated by an orange line in (A) . (C) PCR analysis of xenarthra SIRH11/ZCCHC16 Upper panel: schematic representation of primer design to amplify xenarthra SIRH11/ZCCHC16 . Lower panel: agarose gel electrophoresis profile in each primer set. Ant, giant anteater; Arm, southern three-banded armadillo; Slo, Linnaeus's two-toed sloth; Φx, Φx174 Hae III marker; 1 kb, 1 kb ladder marker.

    Journal: Frontiers in Chemistry

    Article Title: An LTR Retrotransposon-Derived Gene Displays Lineage-Specific Structural and Putative Species-Specific Functional Variations in Eutherians

    doi: 10.3389/fchem.2016.00026

    Figure Lengend Snippet: (A) Common nonsense mutation among xenarthra species. Multiple sequence alignment was constructed using five amino acid sequences; armadillo1, Dasypus novemcinctus ; armadillo2, Tolypeutes matacus ; sloth1, Choloepus hoffmanni ; sloth2, Choloepus didactylus and manatee, Florida manatee as a reference. The red asterisks and Xs show the sites of ORF termination and frameshift, respectively. The blue asterisks in a red box indicate a common mutation among three species. Purple characters indicate CCHC amino acids in the RNA binding domain. (B) DNA alignments around the common TAG nonsense mutation (red) indicated by an orange line in (A) . (C) PCR analysis of xenarthra SIRH11/ZCCHC16 Upper panel: schematic representation of primer design to amplify xenarthra SIRH11/ZCCHC16 . Lower panel: agarose gel electrophoresis profile in each primer set. Ant, giant anteater; Arm, southern three-banded armadillo; Slo, Linnaeus's two-toed sloth; Φx, Φx174 Hae III marker; 1 kb, 1 kb ladder marker.

    Article Snippet: The PCR reaction was performed using PrimeSTAR GXL DNA Polymerase (TaKaRa, Japan) with the following conditions: 94°C, 2 min; 36 cycles of 98°C, 10 s; 55°C, 15 s; 68°C, 20 s; final extention 68°C, 60 s. The following PCR primers were used: Gorilla_SIRH11_F1: 5′-GAGGGAGGAGAGAAAGGTACTG-3′ and Gorilla_SIRH11_R1: 5′-TCTGAGCAATTGGCAGGGTC-3′.

    Techniques: Mutagenesis, Sequencing, Construct, RNA Binding Assay, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker

    Agarose gel electrophoresis of DNA amplified from various genomic DNA extracts of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M2-F and M2-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Agarose gel electrophoresis of DNA amplified from various genomic DNA extracts of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M2-F and M2-R.

    Article Snippet: The PCR reaction was performed in the DNA Engine Gradient Cycler (Bio-Rad, USA).

    Techniques: Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction

    Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M5-F and M5-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M5-F and M5-R.

    Article Snippet: The PCR reaction was performed in the DNA Engine Gradient Cycler (Bio-Rad, USA).

    Techniques: Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction

    Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of ten Korean native honey (KNH) products in duplex PCR with the KNH- specific primers, C6-F and C5-R and the European honey-specific primers, M5-F and M5-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of ten Korean native honey (KNH) products in duplex PCR with the KNH- specific primers, C6-F and C5-R and the European honey-specific primers, M5-F and M5-R.

    Article Snippet: The PCR reaction was performed in the DNA Engine Gradient Cycler (Bio-Rad, USA).

    Techniques: Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction

    Immunochromatographic assay of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R and the EH-specific primers, M5-F and M5-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Immunochromatographic assay of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R and the EH-specific primers, M5-F and M5-R.

    Article Snippet: The PCR reaction was performed in the DNA Engine Gradient Cycler (Bio-Rad, USA).

    Techniques: Immunochromatographic Assay, Amplification, Polymerase Chain Reaction

    Immunochromatographic assay of DNA amplified from genomic DNA extracts of seven Korean native honey (KNH) products in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the European honey(EH)-specific primers, M5-F and M5-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Immunochromatographic assay of DNA amplified from genomic DNA extracts of seven Korean native honey (KNH) products in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the European honey(EH)-specific primers, M5-F and M5-R.

    Article Snippet: The PCR reaction was performed in the DNA Engine Gradient Cycler (Bio-Rad, USA).

    Techniques: Immunochromatographic Assay, Amplification, Polymerase Chain Reaction

    Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M2-F and M2-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M2-F and M2-R.

    Article Snippet: The PCR reaction was performed in the DNA Engine Gradient Cycler (Bio-Rad, USA).

    Techniques: Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction