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  • 95
    Qiagen pcr clean up kit
    TET1 and TET2 influence 5hmC on osteogenic genes. BMSC were cultured under normal growth conditions and treated with scramble siRNA or siRNA directed to TET1 (siTET1) or TET2 (siTET2) and genomic <t>DNA</t> purified and immunoprecipitated using an antibody to 5hmC. Recruitment of 5hmC to genomic regions was assessed by the hme-DIP analysis and normalised to the genomic input control. a Relative enrichment of 5hmC on RUNX2 transcription start site (TSS) was measured using <t>PCR.</t> b 5hmC on BMP2 TSS was measured as in ( a ). BMSC were cultured under normal growth and genomic DNA purified and immunoprecipitated using an antibody to 5hmC. Recruitment of 5hmc to genomic regions was assessed by the hme-DIP analysis and normalised to the genomic input control. c Cells were cultured under normal and osteogenic conditions. Relative enrichment of 5hmC on RUNX2 transcription start site (TSS), exon and intron regions. d Relative enrichment of 5hmC on BMP2 TSS, exon and intron regions, percentage input. Data represent mean S.E.M., n = 3 BMSC donors, * p ≤ 0.05, one-way ANOVA with multiple comparison analyses
    Pcr Clean Up Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1514 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Jena Bioscience pcr purification kit
    Agarose gel electrophoresis Image of HPV DNA genotyping with HPV-16 (457bp) and 18 (322bp) primers. Numbers are samples ID; L is a mid-range ladder (Jena Bioscience); NC is the negative control while PC is the positive control. The sizes of the <t>PCR</t> products generated are 457bp for HPV-16 and 322bp for HPV-18.
    Pcr Purification Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co polymerase chain reaction pcr purification kit
    Effects of inhibitors on the performance of the <t>LF-RPA</t> assay and conventional <t>PCR</t> assay for detecting the DNA from T. spiralis . Different percentage of muscle penetrating fluid in the reaction mixture were evaluated using LF-RPA assay (A) and conventional PCR assay (B) . LF-RPA assay performed better than conventional PCR assay in the presence of potential inhibitors of the amplification reaction. NC, Negative Control.
    Polymerase Chain Reaction Pcr Purification Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche polymerase chain reaction pcr template purification kit
    Effects of inhibitors on the performance of the <t>LF-RPA</t> assay and conventional <t>PCR</t> assay for detecting the DNA from T. spiralis . Different percentage of muscle penetrating fluid in the reaction mixture were evaluated using LF-RPA assay (A) and conventional PCR assay (B) . LF-RPA assay performed better than conventional PCR assay in the presence of potential inhibitors of the amplification reaction. NC, Negative Control.
    Polymerase Chain Reaction Pcr Template Purification Kit, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Qiagen polymerase chain reaction purification kit
    APC is a direct HIF-1α target. (A) ChIP analysis for HIF-1α binding to the APC and CA9 promoters. U2OS cells were exposed to 1% O 2 for the indicated periods before fixation and lysis. HIF-1α-bound <t>DNA</t> was amplified with specific primers spanning the annotated HRE region on the CA9 and the putative HRE at the APC promoter. Rabbit immunoglobulin G (IgG) was used as a control for the immunoprecipitation. Input represents 10% of the starting material used per immunoprecipitation (IP). (B) ChIP analysis for HIF-1β binding at the APC and CA9 promoters. U2OS cells were exposed to 1% O 2 for 24 h before fixation and lysis. HIF-1β–bound DNA was amplified with specific primers spanning the annotated HRE region on the CA9 and the putative HRE at the APC promoter. Rabbit IgG was used as a control for the immunoprecipitation. Input represents 10% of the starting material used per IP. (C) ChIP for AcH3. (D) Polymerase II at the APC HRE site. Cells were treated and processed as described in B. Rabbit IgG was used as a negative control. APC HRE site was amplified using specific <t>PCR</t> primers. Inputs represent 10% of starting material used in each IP.
    Polymerase Chain Reaction Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 81/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Real Biotech Corporation hiyield polymerase chain reaction dna purification kit
    APC is a direct HIF-1α target. (A) ChIP analysis for HIF-1α binding to the APC and CA9 promoters. U2OS cells were exposed to 1% O 2 for the indicated periods before fixation and lysis. HIF-1α-bound <t>DNA</t> was amplified with specific primers spanning the annotated HRE region on the CA9 and the putative HRE at the APC promoter. Rabbit immunoglobulin G (IgG) was used as a control for the immunoprecipitation. Input represents 10% of the starting material used per immunoprecipitation (IP). (B) ChIP analysis for HIF-1β binding at the APC and CA9 promoters. U2OS cells were exposed to 1% O 2 for 24 h before fixation and lysis. HIF-1β–bound DNA was amplified with specific primers spanning the annotated HRE region on the CA9 and the putative HRE at the APC promoter. Rabbit IgG was used as a control for the immunoprecipitation. Input represents 10% of the starting material used per IP. (C) ChIP for AcH3. (D) Polymerase II at the APC HRE site. Cells were treated and processed as described in B. Rabbit IgG was used as a negative control. APC HRE site was amplified using specific <t>PCR</t> primers. Inputs represent 10% of starting material used in each IP.
    Hiyield Polymerase Chain Reaction Dna Purification Kit, supplied by Real Biotech Corporation, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Gentra Systems polymerase chain reaction a genomic dna purification kit
    APC is a direct HIF-1α target. (A) ChIP analysis for HIF-1α binding to the APC and CA9 promoters. U2OS cells were exposed to 1% O 2 for the indicated periods before fixation and lysis. HIF-1α-bound <t>DNA</t> was amplified with specific primers spanning the annotated HRE region on the CA9 and the putative HRE at the APC promoter. Rabbit immunoglobulin G (IgG) was used as a control for the immunoprecipitation. Input represents 10% of the starting material used per immunoprecipitation (IP). (B) ChIP analysis for HIF-1β binding at the APC and CA9 promoters. U2OS cells were exposed to 1% O 2 for 24 h before fixation and lysis. HIF-1β–bound DNA was amplified with specific primers spanning the annotated HRE region on the CA9 and the putative HRE at the APC promoter. Rabbit IgG was used as a control for the immunoprecipitation. Input represents 10% of the starting material used per IP. (C) ChIP for AcH3. (D) Polymerase II at the APC HRE site. Cells were treated and processed as described in B. Rabbit IgG was used as a negative control. APC HRE site was amplified using specific <t>PCR</t> primers. Inputs represent 10% of starting material used in each IP.
    Polymerase Chain Reaction A Genomic Dna Purification Kit, supplied by Gentra Systems, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche polymerase chain reaction pcr purification kits
    Quantitative analysis of vascular endothelial growth factor 2 ( VEGFR2 ) gene expression levels downregulated in AGS cells 72 hours after treatment with anti- VEGFR2 small interfering <t>RNA</t> ( siRNA ). Each real-time polymerase chain reaction <t>(PCR)</t> analysis was carried out at least in triplicate. Data are the fold change in relative expression compared with glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) based on the comparative Ct (2 -ΔΔCt ) method. Values are shown as mean ± SD.
    Polymerase Chain Reaction Pcr Purification Kits, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioteke Corporation polymerase chain reaction pcr purification kit
    Quantitative analysis of vascular endothelial growth factor 2 ( VEGFR2 ) gene expression levels downregulated in AGS cells 72 hours after treatment with anti- VEGFR2 small interfering <t>RNA</t> ( siRNA ). Each real-time polymerase chain reaction <t>(PCR)</t> analysis was carried out at least in triplicate. Data are the fold change in relative expression compared with glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) based on the comparative Ct (2 -ΔΔCt ) method. Values are shown as mean ± SD.
    Polymerase Chain Reaction Pcr Purification Kit, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr purification kit
    Quantitative analysis of vascular endothelial growth factor 2 ( VEGFR2 ) gene expression levels downregulated in AGS cells 72 hours after treatment with anti- VEGFR2 small interfering <t>RNA</t> ( siRNA ). Each real-time polymerase chain reaction <t>(PCR)</t> analysis was carried out at least in triplicate. Data are the fold change in relative expression compared with glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) based on the comparative Ct (2 -ΔΔCt ) method. Values are shown as mean ± SD.
    Polymerase Chain Reaction Pcr Purification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    iNtRON Biotechnology polymerase chain reaction pcr quick spin purification kit
    Quantitative analysis of vascular endothelial growth factor 2 ( VEGFR2 ) gene expression levels downregulated in AGS cells 72 hours after treatment with anti- VEGFR2 small interfering <t>RNA</t> ( siRNA ). Each real-time polymerase chain reaction <t>(PCR)</t> analysis was carried out at least in triplicate. Data are the fold change in relative expression compared with glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) based on the comparative Ct (2 -ΔΔCt ) method. Values are shown as mean ± SD.
    Polymerase Chain Reaction Pcr Quick Spin Purification Kit, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen minelute pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 16148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen minielute polymerase chain reaction pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minielute Polymerase Chain Reaction Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 87/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher genejet pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, <t>GeneJET</t> PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Genejet Pcr Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    MACHEREY NAGEL polymerase chain reaction pcr clean up kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, <t>GeneJET</t> PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Polymerase Chain Reaction Pcr Clean Up Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Biomiga gel polymerase chain reaction pcr extraction kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, <t>GeneJET</t> PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Gel Polymerase Chain Reaction Pcr Extraction Kit, supplied by Biomiga, used in various techniques. Bioz Stars score: 81/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene transcription polymerase chain reaction rt pcr kit
    Detection by reverse transcription–polymerase chain reaction <t>(RT–PCR)</t> of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. <t>cDNA</t> was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.
    Transcription Polymerase Chain Reaction Rt Pcr Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche high pure polymerase chain reaction template preparation kit
    Detection by reverse transcription–polymerase chain reaction <t>(RT–PCR)</t> of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. <t>cDNA</t> was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.
    High Pure Polymerase Chain Reaction Template Preparation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 82/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega polymerase chain reaction pcr nucleospin extract ii kit
    Detection by reverse transcription–polymerase chain reaction <t>(RT–PCR)</t> of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. <t>cDNA</t> was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.
    Polymerase Chain Reaction Pcr Nucleospin Extract Ii Kit, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Axygen axyprep mag polymerase chain reaction pcr clean up kit
    Detection by reverse transcription–polymerase chain reaction <t>(RT–PCR)</t> of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. <t>cDNA</t> was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.
    Axyprep Mag Polymerase Chain Reaction Pcr Clean Up Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa reverse transcription polymerase chain reaction rt pcr kit
    Detection by reverse transcription–polymerase chain reaction <t>(RT–PCR)</t> of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. <t>cDNA</t> was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.
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    Qiagen qiaquick1 polymerase chain reaction pcr extraction kit
    Detection by reverse transcription–polymerase chain reaction <t>(RT–PCR)</t> of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. <t>cDNA</t> was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.
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    TaKaRa in fusion advantage polymerase chain reaction pcr cloning kit
    Detection by reverse transcription–polymerase chain reaction <t>(RT–PCR)</t> of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. <t>cDNA</t> was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.
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    Thermo Fisher quantitative real time polymerase chain reaction rt pcr gene jet rna purification kit
    Detection by reverse transcription–polymerase chain reaction <t>(RT–PCR)</t> of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. <t>cDNA</t> was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.
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    Roche kapa quantitative polymerase chain reaction qpcr kit
    Detection by reverse transcription–polymerase chain reaction <t>(RT–PCR)</t> of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. <t>cDNA</t> was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.
    Kapa Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trizol
    Brucella induces the UPR in vivo . BALB/c mice were injected ip with <t>PBS</t> (NI) or 10 7 B. melitensis ( B. mel ). After 24 h, CD11b+ cells were isolated from pooled spleens and cells were resuspended in <t>Trizol</t> for RNA purification. TNF-α, BiP, CHOP, and ERdj4 gene expression was detected by qPCR with normalization to 18S rRNA. Error bars denote standard deviations between 2 pools (7 mice each). ERdj4 expression is from 1 pool each (NI or B. mel ) of 4 mice. Results represent 2 independent experiments. *p = 0.01.
    Trizol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 250292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher purelink pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, <t>PureLink®</t> PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Purelink Pcr Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2709 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Axygen pcr purification kit
    Removal of <t>PCR</t> inhibitors by the different DNA extraction methods tested. DNA was isolated from 10,000 spores spiked with dust. Resultant DNA was used as template to amplify <t>rDNA</t> sequences. ‘M’ = DNA marker, with the bands representing
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    SolGent pcr purification kit
    <t>PCR</t> performed using the designed primer sets. A, Amplification of marker <t>DNA</t> sequences of the normal (WT) and degenerated strains (M1 and M2); B, Specificity of the primer sets P2-1 and P2-2. The mushroom strains are summarized in Table 1 .
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    GE Healthcare pcr purification kit
    <t>PCR</t> performed using the designed primer sets. A, Amplification of marker <t>DNA</t> sequences of the normal (WT) and degenerated strains (M1 and M2); B, Specificity of the primer sets P2-1 and P2-2. The mushroom strains are summarized in Table 1 .
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    Image Search Results


    TET1 and TET2 influence 5hmC on osteogenic genes. BMSC were cultured under normal growth conditions and treated with scramble siRNA or siRNA directed to TET1 (siTET1) or TET2 (siTET2) and genomic DNA purified and immunoprecipitated using an antibody to 5hmC. Recruitment of 5hmC to genomic regions was assessed by the hme-DIP analysis and normalised to the genomic input control. a Relative enrichment of 5hmC on RUNX2 transcription start site (TSS) was measured using PCR. b 5hmC on BMP2 TSS was measured as in ( a ). BMSC were cultured under normal growth and genomic DNA purified and immunoprecipitated using an antibody to 5hmC. Recruitment of 5hmc to genomic regions was assessed by the hme-DIP analysis and normalised to the genomic input control. c Cells were cultured under normal and osteogenic conditions. Relative enrichment of 5hmC on RUNX2 transcription start site (TSS), exon and intron regions. d Relative enrichment of 5hmC on BMP2 TSS, exon and intron regions, percentage input. Data represent mean S.E.M., n = 3 BMSC donors, * p ≤ 0.05, one-way ANOVA with multiple comparison analyses

    Journal: Epigenetics & Chromatin

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination

    doi: 10.1186/s13072-018-0247-4

    Figure Lengend Snippet: TET1 and TET2 influence 5hmC on osteogenic genes. BMSC were cultured under normal growth conditions and treated with scramble siRNA or siRNA directed to TET1 (siTET1) or TET2 (siTET2) and genomic DNA purified and immunoprecipitated using an antibody to 5hmC. Recruitment of 5hmC to genomic regions was assessed by the hme-DIP analysis and normalised to the genomic input control. a Relative enrichment of 5hmC on RUNX2 transcription start site (TSS) was measured using PCR. b 5hmC on BMP2 TSS was measured as in ( a ). BMSC were cultured under normal growth and genomic DNA purified and immunoprecipitated using an antibody to 5hmC. Recruitment of 5hmc to genomic regions was assessed by the hme-DIP analysis and normalised to the genomic input control. c Cells were cultured under normal and osteogenic conditions. Relative enrichment of 5hmC on RUNX2 transcription start site (TSS), exon and intron regions. d Relative enrichment of 5hmC on BMP2 TSS, exon and intron regions, percentage input. Data represent mean S.E.M., n = 3 BMSC donors, * p ≤ 0.05, one-way ANOVA with multiple comparison analyses

    Article Snippet: Immunoprecipitated bead–DNA complex was treated with proteinase K for 3 h at 50 °C in elution buffer and hydroxymethylated DNA purified by using the Qiagen PCR clean-up kit (Qiaquick).

    Techniques: Cell Culture, Purification, Immunoprecipitation, Polymerase Chain Reaction

    Agarose gel electrophoresis Image of HPV DNA genotyping with HPV-16 (457bp) and 18 (322bp) primers. Numbers are samples ID; L is a mid-range ladder (Jena Bioscience); NC is the negative control while PC is the positive control. The sizes of the PCR products generated are 457bp for HPV-16 and 322bp for HPV-18.

    Journal: PLoS ONE

    Article Title: Molecular characterisation of genital human papillomavirus among women in Southwestern, Nigeria

    doi: 10.1371/journal.pone.0224748

    Figure Lengend Snippet: Agarose gel electrophoresis Image of HPV DNA genotyping with HPV-16 (457bp) and 18 (322bp) primers. Numbers are samples ID; L is a mid-range ladder (Jena Bioscience); NC is the negative control while PC is the positive control. The sizes of the PCR products generated are 457bp for HPV-16 and 322bp for HPV-18.

    Article Snippet: The PCR products were purified with a commercially available PCR purification kit (Jena Bioscience, Germany) according to the manufacturer’s instructions.

    Techniques: Agarose Gel Electrophoresis, Negative Control, Positive Control, Polymerase Chain Reaction, Generated

    Effects of inhibitors on the performance of the LF-RPA assay and conventional PCR assay for detecting the DNA from T. spiralis . Different percentage of muscle penetrating fluid in the reaction mixture were evaluated using LF-RPA assay (A) and conventional PCR assay (B) . LF-RPA assay performed better than conventional PCR assay in the presence of potential inhibitors of the amplification reaction. NC, Negative Control.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Rapid and Visual Detection of Trichinella Spp. Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) Assay

    doi: 10.3389/fcimb.2019.00001

    Figure Lengend Snippet: Effects of inhibitors on the performance of the LF-RPA assay and conventional PCR assay for detecting the DNA from T. spiralis . Different percentage of muscle penetrating fluid in the reaction mixture were evaluated using LF-RPA assay (A) and conventional PCR assay (B) . LF-RPA assay performed better than conventional PCR assay in the presence of potential inhibitors of the amplification reaction. NC, Negative Control.

    Article Snippet: After amplification, the RPA amplicon was purified with a PCR Purification Kit (Tiangen Biotech, China) and then examined on a 2.5% agarose gel.

    Techniques: Recombinase Polymerase Amplification, Polymerase Chain Reaction, Amplification, Negative Control

    Sensitivities of the LF-RPA assay and conventional PCR assay for the detection of isolated genomic DNA from T. spiralis . Ten-fold serial dilutions of T. spiralis genomic DNA (1 ng/reaction to 10 fg/reaction) were evaluated by LF-RPA (A) and conventional PCR assay detected by agarose gel electrophoresis (B) . Lower limit of detection can be seen at 100 fg and 1 pg of T. spiralis DNA by LF-RPA assay and conventional PCR assay, respectively. NC, Negative Control.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Rapid and Visual Detection of Trichinella Spp. Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) Assay

    doi: 10.3389/fcimb.2019.00001

    Figure Lengend Snippet: Sensitivities of the LF-RPA assay and conventional PCR assay for the detection of isolated genomic DNA from T. spiralis . Ten-fold serial dilutions of T. spiralis genomic DNA (1 ng/reaction to 10 fg/reaction) were evaluated by LF-RPA (A) and conventional PCR assay detected by agarose gel electrophoresis (B) . Lower limit of detection can be seen at 100 fg and 1 pg of T. spiralis DNA by LF-RPA assay and conventional PCR assay, respectively. NC, Negative Control.

    Article Snippet: After amplification, the RPA amplicon was purified with a PCR Purification Kit (Tiangen Biotech, China) and then examined on a 2.5% agarose gel.

    Techniques: Recombinase Polymerase Amplification, Polymerase Chain Reaction, Isolation, Agarose Gel Electrophoresis, Negative Control

    Sensitivities of the LF-RPA assay and conventional PCR assay for detecting T. spiralis DNA extracted from swine muscle. One-gram from each muscle sample was spiked with one T. spiralis larvae and 10-fold serial dilutions of this DNA were evaluated by LF-RPA (A) and conventional PCR assay detected by agarose gel electrophoresis (B) . Lower limit of detection can be seen at 1:100 and 1: 10 dilutions of this DNA by LF-RPA assay and conventional PCR assay, respectively. NC, Negative Control.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Rapid and Visual Detection of Trichinella Spp. Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) Assay

    doi: 10.3389/fcimb.2019.00001

    Figure Lengend Snippet: Sensitivities of the LF-RPA assay and conventional PCR assay for detecting T. spiralis DNA extracted from swine muscle. One-gram from each muscle sample was spiked with one T. spiralis larvae and 10-fold serial dilutions of this DNA were evaluated by LF-RPA (A) and conventional PCR assay detected by agarose gel electrophoresis (B) . Lower limit of detection can be seen at 1:100 and 1: 10 dilutions of this DNA by LF-RPA assay and conventional PCR assay, respectively. NC, Negative Control.

    Article Snippet: After amplification, the RPA amplicon was purified with a PCR Purification Kit (Tiangen Biotech, China) and then examined on a 2.5% agarose gel.

    Techniques: Recombinase Polymerase Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Negative Control

    Evaluation of LF-RPA and conventional PCR assay for experimentally infected samples. Et, tongue tissues from experimentally infected group; Ct, tongue tissues from control group; Ed, diaphragm tissues from experimentally infected group; Cd, diaphragm tissues from control group; Ea, abdominal muscle tissues from experimentally infected group; Ca, abdominal muscle tissues from control group.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Rapid and Visual Detection of Trichinella Spp. Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) Assay

    doi: 10.3389/fcimb.2019.00001

    Figure Lengend Snippet: Evaluation of LF-RPA and conventional PCR assay for experimentally infected samples. Et, tongue tissues from experimentally infected group; Ct, tongue tissues from control group; Ed, diaphragm tissues from experimentally infected group; Cd, diaphragm tissues from control group; Ea, abdominal muscle tissues from experimentally infected group; Ca, abdominal muscle tissues from control group.

    Article Snippet: After amplification, the RPA amplicon was purified with a PCR Purification Kit (Tiangen Biotech, China) and then examined on a 2.5% agarose gel.

    Techniques: Recombinase Polymerase Amplification, Polymerase Chain Reaction, Infection

    APC is a direct HIF-1α target. (A) ChIP analysis for HIF-1α binding to the APC and CA9 promoters. U2OS cells were exposed to 1% O 2 for the indicated periods before fixation and lysis. HIF-1α-bound DNA was amplified with specific primers spanning the annotated HRE region on the CA9 and the putative HRE at the APC promoter. Rabbit immunoglobulin G (IgG) was used as a control for the immunoprecipitation. Input represents 10% of the starting material used per immunoprecipitation (IP). (B) ChIP analysis for HIF-1β binding at the APC and CA9 promoters. U2OS cells were exposed to 1% O 2 for 24 h before fixation and lysis. HIF-1β–bound DNA was amplified with specific primers spanning the annotated HRE region on the CA9 and the putative HRE at the APC promoter. Rabbit IgG was used as a control for the immunoprecipitation. Input represents 10% of the starting material used per IP. (C) ChIP for AcH3. (D) Polymerase II at the APC HRE site. Cells were treated and processed as described in B. Rabbit IgG was used as a negative control. APC HRE site was amplified using specific PCR primers. Inputs represent 10% of starting material used in each IP.

    Journal: Molecular Biology of the Cell

    Article Title: Adenomatous Polyposis Coli and Hypoxia-inducible Factor-1? Have an Antagonistic Connection

    doi: 10.1091/mbc.E10-04-0312

    Figure Lengend Snippet: APC is a direct HIF-1α target. (A) ChIP analysis for HIF-1α binding to the APC and CA9 promoters. U2OS cells were exposed to 1% O 2 for the indicated periods before fixation and lysis. HIF-1α-bound DNA was amplified with specific primers spanning the annotated HRE region on the CA9 and the putative HRE at the APC promoter. Rabbit immunoglobulin G (IgG) was used as a control for the immunoprecipitation. Input represents 10% of the starting material used per immunoprecipitation (IP). (B) ChIP analysis for HIF-1β binding at the APC and CA9 promoters. U2OS cells were exposed to 1% O 2 for 24 h before fixation and lysis. HIF-1β–bound DNA was amplified with specific primers spanning the annotated HRE region on the CA9 and the putative HRE at the APC promoter. Rabbit IgG was used as a control for the immunoprecipitation. Input represents 10% of the starting material used per IP. (C) ChIP for AcH3. (D) Polymerase II at the APC HRE site. Cells were treated and processed as described in B. Rabbit IgG was used as a negative control. APC HRE site was amplified using specific PCR primers. Inputs represent 10% of starting material used in each IP.

    Article Snippet: Supernatant was incubated with specific antibodies overnight and then with protein G-Sepharose beads for 1 h. After extensive wash step, the complexes were eluted with buffer (100 mmol/l NaHCO3 and 1% SDS) and incubated with proteinase K. DNA was purified using polymerase chain reaction (PCR) purification kit (QIAGEN/National Blood Service, Burmingham, United Kingdom).

    Techniques: Chromatin Immunoprecipitation, Binding Assay, Lysis, Amplification, Immunoprecipitation, Negative Control, Polymerase Chain Reaction

    Quantitative analysis of vascular endothelial growth factor 2 ( VEGFR2 ) gene expression levels downregulated in AGS cells 72 hours after treatment with anti- VEGFR2 small interfering RNA ( siRNA ). Each real-time polymerase chain reaction (PCR) analysis was carried out at least in triplicate. Data are the fold change in relative expression compared with glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) based on the comparative Ct (2 -ΔΔCt ) method. Values are shown as mean ± SD.

    Journal: Cell Journal (Yakhteh)

    Article Title: Inhibition of AGS Cancer Cell Proliferation following siRNA-Mediated Downregulation of VEGFR2

    doi:

    Figure Lengend Snippet: Quantitative analysis of vascular endothelial growth factor 2 ( VEGFR2 ) gene expression levels downregulated in AGS cells 72 hours after treatment with anti- VEGFR2 small interfering RNA ( siRNA ). Each real-time polymerase chain reaction (PCR) analysis was carried out at least in triplicate. Data are the fold change in relative expression compared with glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) based on the comparative Ct (2 -ΔΔCt ) method. Values are shown as mean ± SD.

    Article Snippet: RNA extraction, cDNA synthesis and polymerase chain reaction (PCR) purification kits were obtained from Roche, Germany.

    Techniques: Expressing, Small Interfering RNA, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    Amplification charts and standard curves for the CT/LGV multiplexed real-time PCR. The linear range of the assay was determined using duplicates of 1.25 × [10 9 , 10 7 , 10 5 , 10 3 , 10 2 , 10 1 ] copies of each cloned amplicon. The threshold values (C t ) were plotted against the corresponding copy numbers, and the efficiency, slope and linear regression correlation (r 2 ) were calculated for each reaction by the Biorad IQ5 software.

    Journal: BMC Infectious Diseases

    Article Title: Rapid Detection of Chlamydia trachomatis and Typing of the Lymphogranuloma venereum associated L-Serovars by TaqMan PCR

    doi: 10.1186/1471-2334-8-56

    Figure Lengend Snippet: Amplification charts and standard curves for the CT/LGV multiplexed real-time PCR. The linear range of the assay was determined using duplicates of 1.25 × [10 9 , 10 7 , 10 5 , 10 3 , 10 2 , 10 1 ] copies of each cloned amplicon. The threshold values (C t ) were plotted against the corresponding copy numbers, and the efficiency, slope and linear regression correlation (r 2 ) were calculated for each reaction by the Biorad IQ5 software.

    Article Snippet: IK_C amplicon and the L2-PCR amplicon were isolated using the PCR purification kit of Macherey and Nagel and 10–20 ng amplicons were ligated into 25 ng pGemT-easy using the rapid DNA ligation kit from Roche following the manufacturer's instructions and subsequently propagated in E. coli DH5α .

    Techniques: Amplification, Real-time Polymerase Chain Reaction, Clone Assay, Software

    (a) Phylogenetic tree of the omp -1-gene region of 20 C. trachomatis serovars targeted in the L1-, L2- and L3-Real-time PCR. (b) Alignments of the respective omp -1-sequences of the serovars A to L grouped in the L1-, L2- or L3-cluster. The accession numbers of the serovars are indicated. Identical nucleotides are boxed, the binding sites of primers and probes are marked above.

    Journal: BMC Infectious Diseases

    Article Title: Rapid Detection of Chlamydia trachomatis and Typing of the Lymphogranuloma venereum associated L-Serovars by TaqMan PCR

    doi: 10.1186/1471-2334-8-56

    Figure Lengend Snippet: (a) Phylogenetic tree of the omp -1-gene region of 20 C. trachomatis serovars targeted in the L1-, L2- and L3-Real-time PCR. (b) Alignments of the respective omp -1-sequences of the serovars A to L grouped in the L1-, L2- or L3-cluster. The accession numbers of the serovars are indicated. Identical nucleotides are boxed, the binding sites of primers and probes are marked above.

    Article Snippet: IK_C amplicon and the L2-PCR amplicon were isolated using the PCR purification kit of Macherey and Nagel and 10–20 ng amplicons were ligated into 25 ng pGemT-easy using the rapid DNA ligation kit from Roche following the manufacturer's instructions and subsequently propagated in E. coli DH5α .

    Techniques: Real-time Polymerase Chain Reaction, Binding Assay

    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Journal: BMC Genomics

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    doi: 10.1186/s12864-017-4371-5

    Figure Lengend Snippet: DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Article Snippet: The ChIP DNA Clean & Concentrator™ (Zymo Research; Zy), the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ne), the MinElute PCR Purification Kit (Qiagen, Qm), the QIAquick PCR Purification Kit (Qiagen; Qp), the Agencourt AMPure XP kit (Beckman; Ba) and the RNAClean™ XP kit (Beckman; Br), and phenol/chloroform extraction (Invitrogen; PC) performed well with de-crosslinked chromatin.

    Techniques: DNA Purification, Chromatin Immunoprecipitation, Purification, Generated, Derivative Assay, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction

    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Journal: BMC Genomics

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    doi: 10.1186/s12864-017-4371-5

    Figure Lengend Snippet: DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Article Snippet: Similarly, DNAs were purified from de-crosslinked chromatin estimated to include 1 ng, 5 ng, 10 ng, and 50 ng of DNA after treatment of RNase A and proteinase K. The following reagents were used in the experiment: ChIP DNA Clean & Concentrator™ (Cat. # D5205) from Zymo Research (Zy) (Irvine, CA); Wizard® SV Gel and PCR Clean-Up System (Cat. # A9281) from Promega (Pr) (Fitchburg, WI); GeneJET PCR Purification Kit (Cat. # K0701) from Thermo Fisher Scientific (Th) (Waltham, MA); PureLink® PCR Purification Kit (Cat. # K310001) from Invitrogen (In) (Carlsbad, CA); Monarch® PCR & DNA Cleanup Kit (Cat. # T1030S) from New England Biolabs (Ne) (Ipswich, MA); Chromatin IP DNA Purification Kit (Cat. # 58002) from Active Motif (Am) (Carlsbad, CA); QIAquick PCR Purification Kit (Cat. # 28106) from Qiagen (Qp) (Valencia, CA), MinElute PCR Purification Kit (Cat. # 28006) from Qiagen (Qm); Agencourt AMPure XP (Cat. # A63881) from Beckman (Ba) (Indianapolis, IN), RNAClean™ XP (Cat. # A63987) from Beckman (Br), and phenol/chloroform extraction (PC) (Additional file ).

    Techniques: DNA Purification, Chromatin Immunoprecipitation, Purification, Generated, Derivative Assay, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction

    Detection by reverse transcription–polymerase chain reaction (RT–PCR) of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. cDNA was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.

    Journal: Immunology

    Article Title: Form and pattern of MUC1 expression on T cells activated in vivo or in vitro suggests a function in T-cell migration

    doi: 10.1046/j.1365-2567.2003.01562.x

    Figure Lengend Snippet: Detection by reverse transcription–polymerase chain reaction (RT–PCR) of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. cDNA was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.

    Article Snippet: Total RNA was prepared using Trizol reagent (Gibco BRL) according to the manufacturer's instructions. cDNA was generated from total RNA using the reverse transcription–polymerase chain reaction (RT–PCR) kit (Stratagene, La Jolla, CA), according to the manufacturer's instructions (in all experiments 10 µg of total RNA was used to generate cDNA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, In Vitro, Positive Control, Molecular Weight, Marker, Purification, Activation Assay, Synthesized

    Brucella induces the UPR in vivo . BALB/c mice were injected ip with PBS (NI) or 10 7 B. melitensis ( B. mel ). After 24 h, CD11b+ cells were isolated from pooled spleens and cells were resuspended in Trizol for RNA purification. TNF-α, BiP, CHOP, and ERdj4 gene expression was detected by qPCR with normalization to 18S rRNA. Error bars denote standard deviations between 2 pools (7 mice each). ERdj4 expression is from 1 pool each (NI or B. mel ) of 4 mice. Results represent 2 independent experiments. *p = 0.01.

    Journal: PLoS Pathogens

    Article Title: Brucella Induces an Unfolded Protein Response via TcpB That Supports Intracellular Replication in Macrophages

    doi: 10.1371/journal.ppat.1003785

    Figure Lengend Snippet: Brucella induces the UPR in vivo . BALB/c mice were injected ip with PBS (NI) or 10 7 B. melitensis ( B. mel ). After 24 h, CD11b+ cells were isolated from pooled spleens and cells were resuspended in Trizol for RNA purification. TNF-α, BiP, CHOP, and ERdj4 gene expression was detected by qPCR with normalization to 18S rRNA. Error bars denote standard deviations between 2 pools (7 mice each). ERdj4 expression is from 1 pool each (NI or B. mel ) of 4 mice. Results represent 2 independent experiments. *p = 0.01.

    Article Snippet: Macrophage cells were infected at 100 MOI and cultured for 24 h. Cells were then washed 1× in PBS, lysed and harvested in 1 ml/well of Trizol (Invitrogen) for RNA processing.

    Techniques: In Vivo, Mouse Assay, Injection, Isolation, Purification, Expressing, Real-time Polymerase Chain Reaction

    Brucella infection activates the UPR in macrophages in vitro . RAW 264.7 macrophages were uninfected (NI) or infected with 100 MOI B. melitensis (B. mel) . A) After 24 h, cells were resuspended in TRIzol for RNA processing. Relative expression of reverse transcribed cDNA was determined by quantitative PCR (qPCR) with normalization to 18S rRNA or hprt. Bars are combined mean fold inductions for 4–5 independent experiments (NI = 1) ± sem. *P

    Journal: PLoS Pathogens

    Article Title: Brucella Induces an Unfolded Protein Response via TcpB That Supports Intracellular Replication in Macrophages

    doi: 10.1371/journal.ppat.1003785

    Figure Lengend Snippet: Brucella infection activates the UPR in macrophages in vitro . RAW 264.7 macrophages were uninfected (NI) or infected with 100 MOI B. melitensis (B. mel) . A) After 24 h, cells were resuspended in TRIzol for RNA processing. Relative expression of reverse transcribed cDNA was determined by quantitative PCR (qPCR) with normalization to 18S rRNA or hprt. Bars are combined mean fold inductions for 4–5 independent experiments (NI = 1) ± sem. *P

    Article Snippet: Macrophage cells were infected at 100 MOI and cultured for 24 h. Cells were then washed 1× in PBS, lysed and harvested in 1 ml/well of Trizol (Invitrogen) for RNA processing.

    Techniques: Infection, In Vitro, Expressing, Real-time Polymerase Chain Reaction

    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Journal: BMC Genomics

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    doi: 10.1186/s12864-017-4371-5

    Figure Lengend Snippet: DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Article Snippet: The Wizard® SV Gel and PCR Clean-Up System (Promega; Pr), the GeneJET PCR Purification Kit (Thermo Fisher Scientific; Th), the PureLink® PCR Purification Kit (Invitrogen; In) and the Chromatin IP DNA Purification Kit (Active Motif; Am) performed poorly with de-crosslinked chromatin.

    Techniques: DNA Purification, Chromatin Immunoprecipitation, Purification, Generated, Derivative Assay, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction

    Removal of PCR inhibitors by the different DNA extraction methods tested. DNA was isolated from 10,000 spores spiked with dust. Resultant DNA was used as template to amplify rDNA sequences. ‘M’ = DNA marker, with the bands representing

    Journal: Journal of environmental monitoring : JEM

    Article Title: Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing

    doi: 10.1039/c2em10779a

    Figure Lengend Snippet: Removal of PCR inhibitors by the different DNA extraction methods tested. DNA was isolated from 10,000 spores spiked with dust. Resultant DNA was used as template to amplify rDNA sequences. ‘M’ = DNA marker, with the bands representing

    Article Snippet: These replicates were then combined and the rDNA amplicons were purified using a PCR purification kit (Axygen, Union City, CA, USA) according to the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, DNA Extraction, Isolation, Marker

    Disposable columns from Qiagen PCR purification and gel extraction kits can be repeatedly regenerated and reused a minimum of five times. ( A ) DNA purification efficacy for the repeatedly regenerated PCR purification kit columns. DNA-contaminated columns were cleaned with 1 M phosphoric acid and used for PCR product (TBox 5) purification; the same columns were regenerated and reused four additional times. The concentration of obtained DNA for each cycle was measured with a Nanophotometer and the data are presented as gel pictures (left) and in a graph (right). ( B ) The analysis of DNA extraction efficacy for the repeatedly regenerated gel extraction kit columns. DNA-contaminated columns were regenerated as in A, where a 50 μL PCR reaction was used for agarose gel electrophoresis. DNA extraction from agarose gel was performed according to the gel extraction kit manual. The data were processed as in A. The full length images for A (left) and B (left) are presented in Supplementary Fig. S4A,B . ( C ) Comparison of DNA purification efficacy between lab-made and commercial buffers. The lab-made buffers (LB) were used for DNA purification for regenerated Qiagen columns, whereas the commercial buffers (CB) were used for DNA purification for the fresh Qiagen or Axygen kit columns. The assays were performed as shown in Fig. 2C . ( D ) Comparison of DNA extraction efficacy between lab-made and commercial buffers. The lab-made buffers (LB) were used for DNA extraction from agarose gel using regenerated Qiagen columns, whereas the commercial buffers (CB) were used for DNA extraction from agarose gel using fresh Qiagen or Axygen kit columns. The assays were performed as shown in Fig. 3B . The protocol for DNA purification with lab-made buffers is described in the materials and methods section. Each column in A (right) and B (right) represents the mean ± SD of three independent experiments.

    Journal: Scientific Reports

    Article Title: Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits

    doi: 10.1038/s41598-018-30316-w

    Figure Lengend Snippet: Disposable columns from Qiagen PCR purification and gel extraction kits can be repeatedly regenerated and reused a minimum of five times. ( A ) DNA purification efficacy for the repeatedly regenerated PCR purification kit columns. DNA-contaminated columns were cleaned with 1 M phosphoric acid and used for PCR product (TBox 5) purification; the same columns were regenerated and reused four additional times. The concentration of obtained DNA for each cycle was measured with a Nanophotometer and the data are presented as gel pictures (left) and in a graph (right). ( B ) The analysis of DNA extraction efficacy for the repeatedly regenerated gel extraction kit columns. DNA-contaminated columns were regenerated as in A, where a 50 μL PCR reaction was used for agarose gel electrophoresis. DNA extraction from agarose gel was performed according to the gel extraction kit manual. The data were processed as in A. The full length images for A (left) and B (left) are presented in Supplementary Fig. S4A,B . ( C ) Comparison of DNA purification efficacy between lab-made and commercial buffers. The lab-made buffers (LB) were used for DNA purification for regenerated Qiagen columns, whereas the commercial buffers (CB) were used for DNA purification for the fresh Qiagen or Axygen kit columns. The assays were performed as shown in Fig. 2C . ( D ) Comparison of DNA extraction efficacy between lab-made and commercial buffers. The lab-made buffers (LB) were used for DNA extraction from agarose gel using regenerated Qiagen columns, whereas the commercial buffers (CB) were used for DNA extraction from agarose gel using fresh Qiagen or Axygen kit columns. The assays were performed as shown in Fig. 3B . The protocol for DNA purification with lab-made buffers is described in the materials and methods section. Each column in A (right) and B (right) represents the mean ± SD of three independent experiments.

    Article Snippet: In addition, similar assays were also performed using regenerated columns from PCR purification kits made by different companies, including Axygen, Tiangen and CWBiotech.

    Techniques: Polymerase Chain Reaction, Purification, Gel Extraction, DNA Purification, Concentration Assay, DNA Extraction, Agarose Gel Electrophoresis

    PCR performed using the designed primer sets. A, Amplification of marker DNA sequences of the normal (WT) and degenerated strains (M1 and M2); B, Specificity of the primer sets P2-1 and P2-2. The mushroom strains are summarized in Table 1 .

    Journal: Mycobiology

    Article Title: Isolation of a Variant Strain of Pleurotus eryngii and the Development of Specific DNA Markers to Identify the Variant Strain

    doi: 10.5941/MYCO.2014.42.1.46

    Figure Lengend Snippet: PCR performed using the designed primer sets. A, Amplification of marker DNA sequences of the normal (WT) and degenerated strains (M1 and M2); B, Specificity of the primer sets P2-1 and P2-2. The mushroom strains are summarized in Table 1 .

    Article Snippet: The PCR products were resolved in 1.5% agarose gels, and unique DNA bands were extracted from the gels and purified using a PCR purification kit (Solgent Co., Daejeon, Korea).

    Techniques: Polymerase Chain Reaction, Amplification, Marker

    (A) Results of real-time PCR with specific primers. (B) Real-time PCR of C005 (left) and C112 (right). (A) Specific primers for C005 (lanes 1 and 2) and C112 (lanes 3 and 4) produced 438- and 380-bp amplicons, respectively. Viral DNA extracted from concentrated

    Journal: Applied and Environmental Microbiology

    Article Title: Amplification of Uncultured Single-Stranded DNA Viruses from Rice Paddy Soil ▿Amplification of Uncultured Single-Stranded DNA Viruses from Rice Paddy Soil ▿ †

    doi: 10.1128/AEM.01275-08

    Figure Lengend Snippet: (A) Results of real-time PCR with specific primers. (B) Real-time PCR of C005 (left) and C112 (right). (A) Specific primers for C005 (lanes 1 and 2) and C112 (lanes 3 and 4) produced 438- and 380-bp amplicons, respectively. Viral DNA extracted from concentrated

    Article Snippet: The linear DNA was purified with PCR purification kits (Solgent, Seoul, Korea).

    Techniques: Real-time Polymerase Chain Reaction, Produced