Journal: BMC Immunology
Article Title: Caries induced cytokine network in the odontoblast layer of human teeth
Figure Lengend Snippet: Quantitative analysis for differential changes in the odontoblast layer (ODL) and pulp of carious teeth . Expression of inflammatory genes in ODL and pulp of normal versus carious teeth was determined by real-time quantitative PCR arrays (A-C). The fold change of gene expression detected by PCR arrays was more robust than that determined by cDNA arrays. However, the overall regulatory profile was similar: the increase of inflammatory gene expression in carious teeth was much more profound in ODL than in the pulp. Values are reported as relative fold change in mRNA transcription of carious versus normal samples. The data represent means and standard errors from triplicate sets of array analyses. Abbreviations: IFNA2, Interferon alpha 2; CEBPB, Enhancer binding protein beta; CRP, C-reactive protein; C4A, complement component 4A; BCL6, B-cell CLL/lymphoma 6; ABCF1, ATP-binding cassette, subfamily F member 1; CD40LG, CD40 ligand; TNF, Tumor necrosis factor alpha; SPP1, Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); LTB4R, Leukotriene B4 receptor; LTB, Lymphotoxin beta; LTA, Lymphotoxin alpha; TOLLIP, Toll-interacting protein; ICEBERG, ICEBERG caspase-1 inhibitor; IL, Interleukin; IL1R1, Interleukin 1 receptor, type I; IL1RN, Interleukin 1 receptor antagonist; IL10RA, interleukin 10 receptor alpha; IL10RB; interleukin 10 receptor beta; IL1F9, Interleukin 1 family member 9; XCR1, Chemokine (C motif) receptor 1; CX3CR1, Chemokine (C-X3-C motif) receptor 1; CCR, Chemokine (C-C motif) receptor; SCYE1, Small inducible cytokine subfamily E member1 (endothelial monocyte activating); MIF, Macrophage migration inhibitory factor; CXCL, Chemokine (C-X-C motif) ligand; CCL, Chemokine (C-C motif) ligand.
Article Snippet: Quantitative Real-Time Polymerase Chain Reaction (qPCR) PCR reagents and all PCR primers for cytokines and receptors except those for detecting β-defensin genes were purchased from SA Biosciences.
Techniques: Expressing, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Binding Assay, Activation Assay, Migration