pcr primer cocktail Illumina Inc Search Results


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  • 99
    New England Biolabs ultra ii dna library prep kit
    Multiplex ligation-dependent probe amplification analysis of ectodermal dysplasia-associated genes. <t>DNA</t> was denatured and hybridized with SALSA probe mix, followed by ligation and polymerase chain reaction amplification. Capillary electrophoresis was performed to generate fragment length and peak area using GeneMapper version 3.0 software. Copy number ratio is denoted as the ratio of peak area of patient vs. peak area of references. Blue color peaks represent the patient sample, whereas the red color peaks were reference.
    Ultra Ii Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1985 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra ii dna library prep kit/product/New England Biolabs
    Average 99 stars, based on 1985 article reviews
    Price from $9.99 to $1999.99
    ultra ii dna library prep kit - by Bioz Stars, 2020-05
    99/100 stars
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    96
    Illumina Inc pcr primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Pcr Primer Cocktail, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr primer cocktail/product/Illumina Inc
    Average 96 stars, based on 843 article reviews
    Price from $9.99 to $1999.99
    pcr primer cocktail - by Bioz Stars, 2020-05
    96/100 stars
      Buy from Supplier

    88
    Illumina Inc truseq pcr primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Truseq Pcr Primer Cocktail, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq pcr primer cocktail/product/Illumina Inc
    Average 88 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    truseq pcr primer cocktail - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    99
    Illumina Inc nextera kit
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Nextera Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 607 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera kit/product/Illumina Inc
    Average 99 stars, based on 607 article reviews
    Price from $9.99 to $1999.99
    nextera kit - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Illumina Inc nextera dna library preparation kit
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Nextera Dna Library Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera dna library preparation kit/product/Illumina Inc
    Average 99 stars, based on 1409 article reviews
    Price from $9.99 to $1999.99
    nextera dna library preparation kit - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    90
    Illumina Inc illumina primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Illumina Primer Cocktail, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina primer cocktail/product/Illumina Inc
    Average 90 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    illumina primer cocktail - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    99
    Illumina Inc rna truseq kit
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Rna Truseq Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna truseq kit/product/Illumina Inc
    Average 99 stars, based on 85 article reviews
    Price from $9.99 to $1999.99
    rna truseq kit - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    85
    Illumina Inc nextera primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Nextera Primer Cocktail, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera primer cocktail/product/Illumina Inc
    Average 85 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    nextera primer cocktail - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    Image Search Results


    Multiplex ligation-dependent probe amplification analysis of ectodermal dysplasia-associated genes. DNA was denatured and hybridized with SALSA probe mix, followed by ligation and polymerase chain reaction amplification. Capillary electrophoresis was performed to generate fragment length and peak area using GeneMapper version 3.0 software. Copy number ratio is denoted as the ratio of peak area of patient vs. peak area of references. Blue color peaks represent the patient sample, whereas the red color peaks were reference.

    Journal: Molecular Medicine Reports

    Article Title: Gene screening facilitates diagnosis of complicated symptoms: A case report

    doi: 10.3892/mmr.2017.7590

    Figure Lengend Snippet: Multiplex ligation-dependent probe amplification analysis of ectodermal dysplasia-associated genes. DNA was denatured and hybridized with SALSA probe mix, followed by ligation and polymerase chain reaction amplification. Capillary electrophoresis was performed to generate fragment length and peak area using GeneMapper version 3.0 software. Copy number ratio is denoted as the ratio of peak area of patient vs. peak area of references. Blue color peaks represent the patient sample, whereas the red color peaks were reference.

    Article Snippet: PCR was performed with a primer cocktail using NEBNext® Ultra™ II DNA Library Prep kit (New England BioLabs, Inc.), followed by purification.

    Techniques: Multiplex Assay, Ligation, Amplification, Polymerase Chain Reaction, Electrophoresis, Software

    The workflow of conventional bisulphite (Bis), oxidative bisulphite (OxBis) and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after PCR amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC

    Journal: Epigenetics & Chromatin

    Article Title: Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA

    doi: 10.1186/s13072-017-0123-7

    Figure Lengend Snippet: The workflow of conventional bisulphite (Bis), oxidative bisulphite (OxBis) and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after PCR amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC

    Article Snippet: After the completion of the OxBis reaction, 5hmC APC PCR product was amplified using PCR primer cocktail supplied by Illumina followed by the clonal Sanger sequencing as described for the M.SssI λDNA control (Additional file : Figure S1B).

    Techniques: Polymerase Chain Reaction, Amplification