pcr primer cocktail Search Results


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  • 99
    New England Biolabs pcr
    Pcr, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 18841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr/product/New England Biolabs
    Average 99 stars, based on 18841 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2020-08
    99/100 stars
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    99
    Thermo Fisher random hexamer primer cocktails
    Random Hexamer Primer Cocktails, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/random hexamer primer cocktails/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    random hexamer primer cocktails - by Bioz Stars, 2020-08
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    95
    Millipore cocktail pcr primer
    Cocktail Pcr Primer, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cocktail pcr primer/product/Millipore
    Average 95 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    cocktail pcr primer - by Bioz Stars, 2020-08
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    92
    Illumina Inc pcr primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Pcr Primer Cocktail, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 1075 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr primer cocktail/product/Illumina Inc
    Average 92 stars, based on 1075 article reviews
    Price from $9.99 to $1999.99
    pcr primer cocktail - by Bioz Stars, 2020-08
    92/100 stars
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    93
    Thermo Fisher pcr primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Pcr Primer Cocktail, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr primer cocktail/product/Thermo Fisher
    Average 93 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    pcr primer cocktail - by Bioz Stars, 2020-08
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    91
    Illumina Inc truseq pcr primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Truseq Pcr Primer Cocktail, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq pcr primer cocktail/product/Illumina Inc
    Average 91 stars, based on 165 article reviews
    Price from $9.99 to $1999.99
    truseq pcr primer cocktail - by Bioz Stars, 2020-08
    91/100 stars
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    91
    takara pcr primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Pcr Primer Cocktail, supplied by takara, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr primer cocktail/product/takara
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr primer cocktail - by Bioz Stars, 2020-08
    91/100 stars
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    89
    Illumina Inc primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Primer Cocktail, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer cocktail/product/Illumina Inc
    Average 89 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    primer cocktail - by Bioz Stars, 2020-08
    89/100 stars
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    85
    Illumina Inc nextera primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Nextera Primer Cocktail, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera primer cocktail/product/Illumina Inc
    Average 85 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    nextera primer cocktail - by Bioz Stars, 2020-08
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    89
    Thermo Fisher primer probe cocktails
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Primer Probe Cocktails, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer probe cocktails/product/Thermo Fisher
    Average 89 stars, based on 45 article reviews
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    primer probe cocktails - by Bioz Stars, 2020-08
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    86
    Schuelke fam labeled m13 primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Fam Labeled M13 Primer Cocktail, supplied by Schuelke, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fam labeled m13 primer cocktail/product/Schuelke
    Average 86 stars, based on 8 article reviews
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    99
    Thermo Fisher random hexamer oligo dt primer cocktail
    Cx43 is degraded by autophagy following Dex treatment ( A ) Inhibition of lysosome activity by CLQ blocked Dex-induced Cx43 degradation. Total cellular proteins (TCPs) from MLO-Y4 cells co-stimulated with 10 −6 M Dex and 50μM CLQ for 24hrs were immunoblotted with antibody against Cx43. β-actin served as loading and normalization control. ( B ) The protein levels of Cx43 were quantified and expressed as a ratio against β-actin. ( C ) Attenuation of autophagy by <t>Atg5</t> silencing blocks Dex-induced Cx43 degradation. TCPs form Atg5 <t>siRNA</t> transfected MLO-Y4 cells with/without Dex treatment for 24hrs were immunoblotted with antibodies against Cx43, Atg5 and LC3-I/II. β-actin served as loading and normalization control. The protein levels Cx43 ( D ) and Atg5 ( E ), were quantified and expressed as a ratio against β-actin. ( F ) The protein levels of lipidated LC3-II were expressed as a ratio against LC3-I. All data presented are representative of at least three independent experiments. *p
    Random Hexamer Oligo Dt Primer Cocktail, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/random hexamer oligo dt primer cocktail/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
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    random hexamer oligo dt primer cocktail - by Bioz Stars, 2020-08
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    85
    Microzone Corp megamix blue pcr cocktail
    Cx43 is degraded by autophagy following Dex treatment ( A ) Inhibition of lysosome activity by CLQ blocked Dex-induced Cx43 degradation. Total cellular proteins (TCPs) from MLO-Y4 cells co-stimulated with 10 −6 M Dex and 50μM CLQ for 24hrs were immunoblotted with antibody against Cx43. β-actin served as loading and normalization control. ( B ) The protein levels of Cx43 were quantified and expressed as a ratio against β-actin. ( C ) Attenuation of autophagy by <t>Atg5</t> silencing blocks Dex-induced Cx43 degradation. TCPs form Atg5 <t>siRNA</t> transfected MLO-Y4 cells with/without Dex treatment for 24hrs were immunoblotted with antibodies against Cx43, Atg5 and LC3-I/II. β-actin served as loading and normalization control. The protein levels Cx43 ( D ) and Atg5 ( E ), were quantified and expressed as a ratio against β-actin. ( F ) The protein levels of lipidated LC3-II were expressed as a ratio against LC3-I. All data presented are representative of at least three independent experiments. *p
    Megamix Blue Pcr Cocktail, supplied by Microzone Corp, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megamix blue pcr cocktail/product/Microzone Corp
    Average 85 stars, based on 14 article reviews
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    megamix blue pcr cocktail - by Bioz Stars, 2020-08
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    94
    Illumina Inc pcr amplification
    Cx43 is degraded by autophagy following Dex treatment ( A ) Inhibition of lysosome activity by CLQ blocked Dex-induced Cx43 degradation. Total cellular proteins (TCPs) from MLO-Y4 cells co-stimulated with 10 −6 M Dex and 50μM CLQ for 24hrs were immunoblotted with antibody against Cx43. β-actin served as loading and normalization control. ( B ) The protein levels of Cx43 were quantified and expressed as a ratio against β-actin. ( C ) Attenuation of autophagy by <t>Atg5</t> silencing blocks Dex-induced Cx43 degradation. TCPs form Atg5 <t>siRNA</t> transfected MLO-Y4 cells with/without Dex treatment for 24hrs were immunoblotted with antibodies against Cx43, Atg5 and LC3-I/II. β-actin served as loading and normalization control. The protein levels Cx43 ( D ) and Atg5 ( E ), were quantified and expressed as a ratio against β-actin. ( F ) The protein levels of lipidated LC3-II were expressed as a ratio against LC3-I. All data presented are representative of at least three independent experiments. *p
    Pcr Amplification, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 12844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr amplification/product/Illumina Inc
    Average 94 stars, based on 12844 article reviews
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    pcr amplification - by Bioz Stars, 2020-08
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    91
    5 PRIME pcr cocktail
    Cx43 is degraded by autophagy following Dex treatment ( A ) Inhibition of lysosome activity by CLQ blocked Dex-induced Cx43 degradation. Total cellular proteins (TCPs) from MLO-Y4 cells co-stimulated with 10 −6 M Dex and 50μM CLQ for 24hrs were immunoblotted with antibody against Cx43. β-actin served as loading and normalization control. ( B ) The protein levels of Cx43 were quantified and expressed as a ratio against β-actin. ( C ) Attenuation of autophagy by <t>Atg5</t> silencing blocks Dex-induced Cx43 degradation. TCPs form Atg5 <t>siRNA</t> transfected MLO-Y4 cells with/without Dex treatment for 24hrs were immunoblotted with antibodies against Cx43, Atg5 and LC3-I/II. β-actin served as loading and normalization control. The protein levels Cx43 ( D ) and Atg5 ( E ), were quantified and expressed as a ratio against β-actin. ( F ) The protein levels of lipidated LC3-II were expressed as a ratio against LC3-I. All data presented are representative of at least three independent experiments. *p
    Pcr Cocktail, supplied by 5 PRIME, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr cocktail/product/5 PRIME
    Average 91 stars, based on 18 article reviews
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    pcr cocktail - by Bioz Stars, 2020-08
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    97
    Thermo Fisher platinum quantitative pcr supermix udg cocktail
    Cx43 is degraded by autophagy following Dex treatment ( A ) Inhibition of lysosome activity by CLQ blocked Dex-induced Cx43 degradation. Total cellular proteins (TCPs) from MLO-Y4 cells co-stimulated with 10 −6 M Dex and 50μM CLQ for 24hrs were immunoblotted with antibody against Cx43. β-actin served as loading and normalization control. ( B ) The protein levels of Cx43 were quantified and expressed as a ratio against β-actin. ( C ) Attenuation of autophagy by <t>Atg5</t> silencing blocks Dex-induced Cx43 degradation. TCPs form Atg5 <t>siRNA</t> transfected MLO-Y4 cells with/without Dex treatment for 24hrs were immunoblotted with antibodies against Cx43, Atg5 and LC3-I/II. β-actin served as loading and normalization control. The protein levels Cx43 ( D ) and Atg5 ( E ), were quantified and expressed as a ratio against β-actin. ( F ) The protein levels of lipidated LC3-II were expressed as a ratio against LC3-I. All data presented are representative of at least three independent experiments. *p
    Platinum Quantitative Pcr Supermix Udg Cocktail, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum quantitative pcr supermix udg cocktail/product/Thermo Fisher
    Average 97 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    platinum quantitative pcr supermix udg cocktail - by Bioz Stars, 2020-08
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    99
    Illumina Inc nextera kit
    Cx43 is degraded by autophagy following Dex treatment ( A ) Inhibition of lysosome activity by CLQ blocked Dex-induced Cx43 degradation. Total cellular proteins (TCPs) from MLO-Y4 cells co-stimulated with 10 −6 M Dex and 50μM CLQ for 24hrs were immunoblotted with antibody against Cx43. β-actin served as loading and normalization control. ( B ) The protein levels of Cx43 were quantified and expressed as a ratio against β-actin. ( C ) Attenuation of autophagy by <t>Atg5</t> silencing blocks Dex-induced Cx43 degradation. TCPs form Atg5 <t>siRNA</t> transfected MLO-Y4 cells with/without Dex treatment for 24hrs were immunoblotted with antibodies against Cx43, Atg5 and LC3-I/II. β-actin served as loading and normalization control. The protein levels Cx43 ( D ) and Atg5 ( E ), were quantified and expressed as a ratio against β-actin. ( F ) The protein levels of lipidated LC3-II were expressed as a ratio against LC3-I. All data presented are representative of at least three independent experiments. *p
    Nextera Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 777 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera kit/product/Illumina Inc
    Average 99 stars, based on 777 article reviews
    Price from $9.99 to $1999.99
    nextera kit - by Bioz Stars, 2020-08
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    99
    Illumina Inc nextera dna library preparation kit
    Cx43 is degraded by autophagy following Dex treatment ( A ) Inhibition of lysosome activity by CLQ blocked Dex-induced Cx43 degradation. Total cellular proteins (TCPs) from MLO-Y4 cells co-stimulated with 10 −6 M Dex and 50μM CLQ for 24hrs were immunoblotted with antibody against Cx43. β-actin served as loading and normalization control. ( B ) The protein levels of Cx43 were quantified and expressed as a ratio against β-actin. ( C ) Attenuation of autophagy by <t>Atg5</t> silencing blocks Dex-induced Cx43 degradation. TCPs form Atg5 <t>siRNA</t> transfected MLO-Y4 cells with/without Dex treatment for 24hrs were immunoblotted with antibodies against Cx43, Atg5 and LC3-I/II. β-actin served as loading and normalization control. The protein levels Cx43 ( D ) and Atg5 ( E ), were quantified and expressed as a ratio against β-actin. ( F ) The protein levels of lipidated LC3-II were expressed as a ratio against LC3-I. All data presented are representative of at least three independent experiments. *p
    Nextera Dna Library Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1568 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera dna library preparation kit/product/Illumina Inc
    Average 99 stars, based on 1568 article reviews
    Price from $9.99 to $1999.99
    nextera dna library preparation kit - by Bioz Stars, 2020-08
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    Image Search Results


    The workflow of conventional bisulphite (Bis), oxidative bisulphite (OxBis) and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after PCR amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC

    Journal: Epigenetics & Chromatin

    Article Title: Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA

    doi: 10.1186/s13072-017-0123-7

    Figure Lengend Snippet: The workflow of conventional bisulphite (Bis), oxidative bisulphite (OxBis) and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after PCR amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC

    Article Snippet: After the completion of the OxBis reaction, 5hmC APC PCR product was amplified using PCR primer cocktail supplied by Illumina followed by the clonal Sanger sequencing as described for the M.SssI λDNA control (Additional file : Figure S1B).

    Techniques: Polymerase Chain Reaction, Amplification

    Cx43 is degraded by autophagy following Dex treatment ( A ) Inhibition of lysosome activity by CLQ blocked Dex-induced Cx43 degradation. Total cellular proteins (TCPs) from MLO-Y4 cells co-stimulated with 10 −6 M Dex and 50μM CLQ for 24hrs were immunoblotted with antibody against Cx43. β-actin served as loading and normalization control. ( B ) The protein levels of Cx43 were quantified and expressed as a ratio against β-actin. ( C ) Attenuation of autophagy by Atg5 silencing blocks Dex-induced Cx43 degradation. TCPs form Atg5 siRNA transfected MLO-Y4 cells with/without Dex treatment for 24hrs were immunoblotted with antibodies against Cx43, Atg5 and LC3-I/II. β-actin served as loading and normalization control. The protein levels Cx43 ( D ) and Atg5 ( E ), were quantified and expressed as a ratio against β-actin. ( F ) The protein levels of lipidated LC3-II were expressed as a ratio against LC3-I. All data presented are representative of at least three independent experiments. *p

    Journal: Oncotarget

    Article Title: Glucocorticoid impairs cell-cell communication by autophagy-mediated degradation of connexin 43 in osteocytes

    doi: 10.18632/oncotarget.9034

    Figure Lengend Snippet: Cx43 is degraded by autophagy following Dex treatment ( A ) Inhibition of lysosome activity by CLQ blocked Dex-induced Cx43 degradation. Total cellular proteins (TCPs) from MLO-Y4 cells co-stimulated with 10 −6 M Dex and 50μM CLQ for 24hrs were immunoblotted with antibody against Cx43. β-actin served as loading and normalization control. ( B ) The protein levels of Cx43 were quantified and expressed as a ratio against β-actin. ( C ) Attenuation of autophagy by Atg5 silencing blocks Dex-induced Cx43 degradation. TCPs form Atg5 siRNA transfected MLO-Y4 cells with/without Dex treatment for 24hrs were immunoblotted with antibodies against Cx43, Atg5 and LC3-I/II. β-actin served as loading and normalization control. The protein levels Cx43 ( D ) and Atg5 ( E ), were quantified and expressed as a ratio against β-actin. ( F ) The protein levels of lipidated LC3-II were expressed as a ratio against LC3-I. All data presented are representative of at least three independent experiments. *p

    Article Snippet: Transfection MLO-Y4 cells (1×105 /well) were plated in type-I collagen coated six-well culture plates, and then transfected with various concentrations (12.5nM, 25nM, 50nM and 100nM) of Atg5 siRNA oligo cocktail (Stealth siRNAs: MSS247019, MSS247020, MSS247021, from Thermo Fisher® , USA) or negative control using Lipofectamine® 3000 Transfection Reagent (Invitrogen® , USA) following the manufacturer's protocol.

    Techniques: Inhibition, Activity Assay, Transfection

    ID1 siRNA reduces docetaxel sensitivity in the prostate cancer C42B cell line. (A) Representative western blots of ID1 in LNCaP and C42B cell lines ( top ) and after C42B cells were treated by DTX at the indicated doses for 48 hours ( bottom ). (B) C42B cells

    Journal: Cancer research

    Article Title: ID1 enhances docetaxel cytotoxicity in prostate cancer cells via inhibition of p21

    doi: 10.1158/0008-5472.CAN-09-3186

    Figure Lengend Snippet: ID1 siRNA reduces docetaxel sensitivity in the prostate cancer C42B cell line. (A) Representative western blots of ID1 in LNCaP and C42B cell lines ( top ) and after C42B cells were treated by DTX at the indicated doses for 48 hours ( bottom ). (B) C42B cells

    Article Snippet: An oligo-based ID1 siRNA cocktail (SMARTpoll siRNA) and transfection reagent Dharmafect 3 were purchased from Thermo Scientific Dharmacon (Lafayette, CO).

    Techniques: Western Blot

    ID1 siRNA reduces docetaxel cytotoxicity in the LNCaP cell line. (A) Top : Representative western blot of ID1 expression in LNCaP cells treated with siRNA non-targeting control (si-Control) or siRNA ID1 (si-ID1) for 2 days, followed by 20 nM of DTX or

    Journal: Cancer research

    Article Title: ID1 enhances docetaxel cytotoxicity in prostate cancer cells via inhibition of p21

    doi: 10.1158/0008-5472.CAN-09-3186

    Figure Lengend Snippet: ID1 siRNA reduces docetaxel cytotoxicity in the LNCaP cell line. (A) Top : Representative western blot of ID1 expression in LNCaP cells treated with siRNA non-targeting control (si-Control) or siRNA ID1 (si-ID1) for 2 days, followed by 20 nM of DTX or

    Article Snippet: An oligo-based ID1 siRNA cocktail (SMARTpoll siRNA) and transfection reagent Dharmafect 3 were purchased from Thermo Scientific Dharmacon (Lafayette, CO).

    Techniques: Western Blot, Expressing