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  • 99
    Thermo Fisher polymerase chain reaction pcr mixture
    Polymerase Chain Reaction Pcr Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer polymerase chain reaction pcr mixture
    Polymerase Chain Reaction Pcr Mixture, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr mixture
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co polymerase chain reaction pcr mixture
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Mixture, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare polymerase chain reaction pcr mixtures
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Mixtures, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Stratagene polymerase chain reaction pcr mixtures
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Mixtures, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr mixtures
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Mixtures, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MJ Research polymerase chain reaction reaction mixtures
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Reaction Mixtures, supplied by MJ Research, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche polymerase chain reaction pcr reaction mixtures
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Reaction Mixtures, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polymerase chain reaction pcr reaction mixtures
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Reaction Mixtures, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pcr reaction mixture
    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by <t>SYBR-Green</t> I real-time <t>PCR.</t> The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.
    Pcr Reaction Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 2570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polymerase chain reaction mixtures
    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by <t>SYBR-Green</t> I real-time <t>PCR.</t> The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.
    Polymerase Chain Reaction Mixtures, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG polymerase chain reaction mixtures
    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by <t>SYBR-Green</t> I real-time <t>PCR.</t> The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.
    Polymerase Chain Reaction Mixtures, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche pcr mixtures
    <t>RT-PCR</t> analysis of viral SV2neo PPA-F3 RNAs. Total RNA was extracted from transiently transfected 293T cells, treated with DNase I, and subjected to RT-PCR to search for the presence of (A) nonspliced gag (498-bp) or (B, C) doubly spliced tax/rex (424-bp) viral messengers. (A) Lane 1, 100-bp <t>DNA</t> ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with SV2neo PPA-F3 clones 6, 7, and 26, respectively (these three recombinant plasmids contain the wild-type SV2neo PPA-F3 sequence). For lane 7, RT was not added to the PCR mix containing RNA extracted from clone 26-transfected cells. (B) RT-PCR strategy for amplifying the rex ). (C) RNAs were tested for the presence of a band corresponding to the spliced tax / rex mRNA transcript. Lane 1, 100-bp DNA ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with STLV-3 PPA-F3 clones 6, 7, and 26, respectively. NS, nonspecific band. (D) Sequence analysis of the 424-bp-long RT-PCR product. The gag and tax / rex gels are representative of at least three different experiments performed on different RNA preparations. Numbers to the left of the blots are in base pairs.
    Pcr Mixtures, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 706 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co polymerase chain reaction pcr mixtures
    <t>RT-PCR</t> analysis of viral SV2neo PPA-F3 RNAs. Total RNA was extracted from transiently transfected 293T cells, treated with DNase I, and subjected to RT-PCR to search for the presence of (A) nonspliced gag (498-bp) or (B, C) doubly spliced tax/rex (424-bp) viral messengers. (A) Lane 1, 100-bp <t>DNA</t> ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with SV2neo PPA-F3 clones 6, 7, and 26, respectively (these three recombinant plasmids contain the wild-type SV2neo PPA-F3 sequence). For lane 7, RT was not added to the PCR mix containing RNA extracted from clone 26-transfected cells. (B) RT-PCR strategy for amplifying the rex ). (C) RNAs were tested for the presence of a band corresponding to the spliced tax / rex mRNA transcript. Lane 1, 100-bp DNA ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with STLV-3 PPA-F3 clones 6, 7, and 26, respectively. NS, nonspecific band. (D) Sequence analysis of the 424-bp-long RT-PCR product. The gag and tax / rex gels are representative of at least three different experiments performed on different RNA preparations. Numbers to the left of the blots are in base pairs.
    Polymerase Chain Reaction Pcr Mixtures, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems polymerase chain reaction pcr mixtures
    <t>RT-PCR</t> analysis of viral SV2neo PPA-F3 RNAs. Total RNA was extracted from transiently transfected 293T cells, treated with DNase I, and subjected to RT-PCR to search for the presence of (A) nonspliced gag (498-bp) or (B, C) doubly spliced tax/rex (424-bp) viral messengers. (A) Lane 1, 100-bp <t>DNA</t> ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with SV2neo PPA-F3 clones 6, 7, and 26, respectively (these three recombinant plasmids contain the wild-type SV2neo PPA-F3 sequence). For lane 7, RT was not added to the PCR mix containing RNA extracted from clone 26-transfected cells. (B) RT-PCR strategy for amplifying the rex ). (C) RNAs were tested for the presence of a band corresponding to the spliced tax / rex mRNA transcript. Lane 1, 100-bp DNA ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with STLV-3 PPA-F3 clones 6, 7, and 26, respectively. NS, nonspecific band. (D) Sequence analysis of the 424-bp-long RT-PCR product. The gag and tax / rex gels are representative of at least three different experiments performed on different RNA preparations. Numbers to the left of the blots are in base pairs.
    Polymerase Chain Reaction Pcr Mixtures, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meridian Life Science polymerase chain reaction pcr mixtures
    <t>RT-PCR</t> analysis of viral SV2neo PPA-F3 RNAs. Total RNA was extracted from transiently transfected 293T cells, treated with DNase I, and subjected to RT-PCR to search for the presence of (A) nonspliced gag (498-bp) or (B, C) doubly spliced tax/rex (424-bp) viral messengers. (A) Lane 1, 100-bp <t>DNA</t> ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with SV2neo PPA-F3 clones 6, 7, and 26, respectively (these three recombinant plasmids contain the wild-type SV2neo PPA-F3 sequence). For lane 7, RT was not added to the PCR mix containing RNA extracted from clone 26-transfected cells. (B) RT-PCR strategy for amplifying the rex ). (C) RNAs were tested for the presence of a band corresponding to the spliced tax / rex mRNA transcript. Lane 1, 100-bp DNA ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with STLV-3 PPA-F3 clones 6, 7, and 26, respectively. NS, nonspecific band. (D) Sequence analysis of the 424-bp-long RT-PCR product. The gag and tax / rex gels are representative of at least three different experiments performed on different RNA preparations. Numbers to the left of the blots are in base pairs.
    Polymerase Chain Reaction Pcr Mixtures, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Roche pcr reaction mixture
    <t>RT-PCR</t> analysis of viral SV2neo PPA-F3 RNAs. Total RNA was extracted from transiently transfected 293T cells, treated with DNase I, and subjected to RT-PCR to search for the presence of (A) nonspliced gag (498-bp) or (B, C) doubly spliced tax/rex (424-bp) viral messengers. (A) Lane 1, 100-bp <t>DNA</t> ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with SV2neo PPA-F3 clones 6, 7, and 26, respectively (these three recombinant plasmids contain the wild-type SV2neo PPA-F3 sequence). For lane 7, RT was not added to the PCR mix containing RNA extracted from clone 26-transfected cells. (B) RT-PCR strategy for amplifying the rex ). (C) RNAs were tested for the presence of a band corresponding to the spliced tax / rex mRNA transcript. Lane 1, 100-bp DNA ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with STLV-3 PPA-F3 clones 6, 7, and 26, respectively. NS, nonspecific band. (D) Sequence analysis of the 424-bp-long RT-PCR product. The gag and tax / rex gels are representative of at least three different experiments performed on different RNA preparations. Numbers to the left of the blots are in base pairs.
    Pcr Reaction Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 1143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad pcr mixture
    Inhibition of vaccinia virus transcription by Tkip and SOCS1-KIR. BSC-40 cells mock treated or treated with lipo-Tkip, lipo-SOCS1-KIR, or alanine substitution-containing control peptides for 1 h were infected with vaccinia virus at an MOI of 5 for 1 h. The cells were washed and incubated in growth medium containing the same concentration of peptides for 18 h. RNA was extracted and used for <t>cDNA</t> synthesis, followed by quantitative <t>PCR.</t> The expression of early (D12L) (a), intermediate (AIL) (b), and late (A7L) (c) genes was compared with endogenous actin gene expression.
    Pcr Mixture, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1531 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche pcr mixture
    Application of DNA polymerase from P. <t>islandicum</t> in <t>PCR.</t> Reactions were carried out in a buffer containing 15 mM Tris-HCl (pH 8.6), 12.5 mM KCl, 2.5 mM (NH 4 ) 2 SO 4 , 1.25 mM MgCl 2 , and 20 μg of BSA per ml. One unit of DNA polymerase from P. islandicum was used to amplify 500 bp (lane 1), 1,000 bp (lane 2), and 1,500 bp (lane 3) of a λ DNA template. Control PCR was performed with 2.5 U of High Fidelity enzyme (lane 4). Twenty microliters of each PCR product was applied on an 1% agarose gel. The corresponding molecular sizes of the marker (lane 5) are indicated in kilobase pairs.
    Pcr Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 2412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene polymerase chain reaction mixture
    Application of DNA polymerase from P. <t>islandicum</t> in <t>PCR.</t> Reactions were carried out in a buffer containing 15 mM Tris-HCl (pH 8.6), 12.5 mM KCl, 2.5 mM (NH 4 ) 2 SO 4 , 1.25 mM MgCl 2 , and 20 μg of BSA per ml. One unit of DNA polymerase from P. islandicum was used to amplify 500 bp (lane 1), 1,000 bp (lane 2), and 1,500 bp (lane 3) of a λ DNA template. Control PCR was performed with 2.5 U of High Fidelity enzyme (lane 4). Twenty microliters of each PCR product was applied on an 1% agarose gel. The corresponding molecular sizes of the marker (lane 5) are indicated in kilobase pairs.
    Polymerase Chain Reaction Mixture, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    iNtRON Biotechnology polymerase chain reaction pcr reaction mixture
    Application of DNA polymerase from P. <t>islandicum</t> in <t>PCR.</t> Reactions were carried out in a buffer containing 15 mM Tris-HCl (pH 8.6), 12.5 mM KCl, 2.5 mM (NH 4 ) 2 SO 4 , 1.25 mM MgCl 2 , and 20 μg of BSA per ml. One unit of DNA polymerase from P. islandicum was used to amplify 500 bp (lane 1), 1,000 bp (lane 2), and 1,500 bp (lane 3) of a λ DNA template. Control PCR was performed with 2.5 U of High Fidelity enzyme (lane 4). Twenty microliters of each PCR product was applied on an 1% agarose gel. The corresponding molecular sizes of the marker (lane 5) are indicated in kilobase pairs.
    Polymerase Chain Reaction Pcr Reaction Mixture, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control PCR was performed under the same conditions except for without template DNA (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

    Journal: BioMed Research International

    Article Title: Population Abundance of Potentially Pathogenic Organisms in Intestinal Microbiome of Jungle Crow (Corvus macrorhynchos) Shown with 16S rRNA Gene-Based Microbial Community Analysis

    doi: 10.1155/2013/438956

    Figure Lengend Snippet: Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control PCR was performed under the same conditions except for without template DNA (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

    Article Snippet: PCR Conditions Twenty-five to fifty nanograms of isolated DNA were transferred to a PCR tube containing 50 μ L of a polymerase chain reaction (PCR) mixture containing LA Taq DNA polymerase (TaKaRa Bio, Shiga, Japan).

    Techniques: Amplification, Polymerase Chain Reaction, Marker

    The expression of the S. sq VEGFR gene in the H. roretzi tissues is correlated with the existence of circulatory system. ( a ) RT-PCR analysis of S. sq VEGFR gene expression in the different H. roretzi tissues. Upper panel: PCR was performed using a S. sq VEGFR -specific primer set and reverse-transcribed cDNA from the total RNA of each tissue as the template. Lower panel: PCR was preformed using the H. roretzi β-actin-specific primer set and reverse-transcribed cDNA from total RNA of each tissue as the template (positive control); ( b ) Anatomical scheme of H. roretzi .

    Journal: International Journal of Molecular Sciences

    Article Title: Vascular Endothelial Growth Factor Receptor Family in Ascidians, Halocynthia roretzi (Sea Squirt). Its High Expression in Circulatory System-Containing Tissues

    doi: 10.3390/ijms14034841

    Figure Lengend Snippet: The expression of the S. sq VEGFR gene in the H. roretzi tissues is correlated with the existence of circulatory system. ( a ) RT-PCR analysis of S. sq VEGFR gene expression in the different H. roretzi tissues. Upper panel: PCR was performed using a S. sq VEGFR -specific primer set and reverse-transcribed cDNA from the total RNA of each tissue as the template. Lower panel: PCR was preformed using the H. roretzi β-actin-specific primer set and reverse-transcribed cDNA from total RNA of each tissue as the template (positive control); ( b ) Anatomical scheme of H. roretzi .

    Article Snippet: The PCR mixture contained reverse-transcribed DNA from the total RNA samples of ascidian tissues, Fw primer (50 pmol), Rw primer (50 pmol), dNTPs (20 pmol), PCR buffer, and rTaq DNA polymerase (TaKaRa) in 100 μL.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Positive Control

    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by SYBR-Green I real-time PCR. The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.

    Journal: Oncology Letters

    Article Title: Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer

    doi: 10.3892/ol.2012.714

    Figure Lengend Snippet: Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by SYBR-Green I real-time PCR. The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.

    Article Snippet: The 25 μl PCR reaction mixture included 1× SYBR premix Ex Taq mix (Takara), 2 μl RT products and 10 nM of each forward and reverse primer.

    Techniques: Plasmid Preparation, SYBR Green Assay, Real-time Polymerase Chain Reaction, Produced

    RT-PCR analysis of viral SV2neo PPA-F3 RNAs. Total RNA was extracted from transiently transfected 293T cells, treated with DNase I, and subjected to RT-PCR to search for the presence of (A) nonspliced gag (498-bp) or (B, C) doubly spliced tax/rex (424-bp) viral messengers. (A) Lane 1, 100-bp DNA ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with SV2neo PPA-F3 clones 6, 7, and 26, respectively (these three recombinant plasmids contain the wild-type SV2neo PPA-F3 sequence). For lane 7, RT was not added to the PCR mix containing RNA extracted from clone 26-transfected cells. (B) RT-PCR strategy for amplifying the rex ). (C) RNAs were tested for the presence of a band corresponding to the spliced tax / rex mRNA transcript. Lane 1, 100-bp DNA ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with STLV-3 PPA-F3 clones 6, 7, and 26, respectively. NS, nonspecific band. (D) Sequence analysis of the 424-bp-long RT-PCR product. The gag and tax / rex gels are representative of at least three different experiments performed on different RNA preparations. Numbers to the left of the blots are in base pairs.

    Journal: Journal of Virology

    Article Title: Construction and Characterization of a Full-Length Infectious Simian T-Cell Lymphotropic Virus Type 3 Molecular Clone ▿

    doi: 10.1128/JVI.02538-06

    Figure Lengend Snippet: RT-PCR analysis of viral SV2neo PPA-F3 RNAs. Total RNA was extracted from transiently transfected 293T cells, treated with DNase I, and subjected to RT-PCR to search for the presence of (A) nonspliced gag (498-bp) or (B, C) doubly spliced tax/rex (424-bp) viral messengers. (A) Lane 1, 100-bp DNA ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with SV2neo PPA-F3 clones 6, 7, and 26, respectively (these three recombinant plasmids contain the wild-type SV2neo PPA-F3 sequence). For lane 7, RT was not added to the PCR mix containing RNA extracted from clone 26-transfected cells. (B) RT-PCR strategy for amplifying the rex ). (C) RNAs were tested for the presence of a band corresponding to the spliced tax / rex mRNA transcript. Lane 1, 100-bp DNA ladder; lane 2, mock-transfected cells; lane 3, RNA from SV2neo-transfected cells; lanes 4 to 6, RNA from cells transfected with STLV-3 PPA-F3 clones 6, 7, and 26, respectively. NS, nonspecific band. (D) Sequence analysis of the 424-bp-long RT-PCR product. The gag and tax / rex gels are representative of at least three different experiments performed on different RNA preparations. Numbers to the left of the blots are in base pairs.

    Article Snippet: The PCR mixtures contained 1 μg of DNA, 5 μl 10× Pfu buffer, a 0.2 mM concentration of each deoxynucleoside triphosphate (Roche), 2.5 U PfuTurbo DNA polymerase (Stratagene), and 10 pmol of each primer.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Transfection, Clone Assay, Recombinant, Sequencing, Polymerase Chain Reaction

    Inhibition of vaccinia virus transcription by Tkip and SOCS1-KIR. BSC-40 cells mock treated or treated with lipo-Tkip, lipo-SOCS1-KIR, or alanine substitution-containing control peptides for 1 h were infected with vaccinia virus at an MOI of 5 for 1 h. The cells were washed and incubated in growth medium containing the same concentration of peptides for 18 h. RNA was extracted and used for cDNA synthesis, followed by quantitative PCR. The expression of early (D12L) (a), intermediate (AIL) (b), and late (A7L) (c) genes was compared with endogenous actin gene expression.

    Journal: Journal of Virology

    Article Title: SOCS-1 Mimetics Protect Mice against Lethal Poxvirus Infection: Identification of a Novel Endogenous Antiviral System ▿

    doi: 10.1128/JVI.01138-08

    Figure Lengend Snippet: Inhibition of vaccinia virus transcription by Tkip and SOCS1-KIR. BSC-40 cells mock treated or treated with lipo-Tkip, lipo-SOCS1-KIR, or alanine substitution-containing control peptides for 1 h were infected with vaccinia virus at an MOI of 5 for 1 h. The cells were washed and incubated in growth medium containing the same concentration of peptides for 18 h. RNA was extracted and used for cDNA synthesis, followed by quantitative PCR. The expression of early (D12L) (a), intermediate (AIL) (b), and late (A7L) (c) genes was compared with endogenous actin gene expression.

    Article Snippet: The cDNA was diluted 1:100, and 1 μl of the diluted cDNA was taken in a 50-μl reaction mixture with primers and a PCR mixture containing SYBR green (Bio-Rad, Hercules, CA) and used for PCR in a Mini Opticon thermal cycler (Bio-Rad, Hercules, CA).

    Techniques: Inhibition, Infection, Incubation, Concentration Assay, Real-time Polymerase Chain Reaction, Expressing

    Application of DNA polymerase from P. islandicum in PCR. Reactions were carried out in a buffer containing 15 mM Tris-HCl (pH 8.6), 12.5 mM KCl, 2.5 mM (NH 4 ) 2 SO 4 , 1.25 mM MgCl 2 , and 20 μg of BSA per ml. One unit of DNA polymerase from P. islandicum was used to amplify 500 bp (lane 1), 1,000 bp (lane 2), and 1,500 bp (lane 3) of a λ DNA template. Control PCR was performed with 2.5 U of High Fidelity enzyme (lane 4). Twenty microliters of each PCR product was applied on an 1% agarose gel. The corresponding molecular sizes of the marker (lane 5) are indicated in kilobase pairs.

    Journal: Journal of Bacteriology

    Article Title: Cloning and Characterization of a Family B DNA Polymerase from the Hyperthermophilic Crenarchaeon Pyrobaculum islandicum †

    doi:

    Figure Lengend Snippet: Application of DNA polymerase from P. islandicum in PCR. Reactions were carried out in a buffer containing 15 mM Tris-HCl (pH 8.6), 12.5 mM KCl, 2.5 mM (NH 4 ) 2 SO 4 , 1.25 mM MgCl 2 , and 20 μg of BSA per ml. One unit of DNA polymerase from P. islandicum was used to amplify 500 bp (lane 1), 1,000 bp (lane 2), and 1,500 bp (lane 3) of a λ DNA template. Control PCR was performed with 2.5 U of High Fidelity enzyme (lane 4). Twenty microliters of each PCR product was applied on an 1% agarose gel. The corresponding molecular sizes of the marker (lane 5) are indicated in kilobase pairs.

    Article Snippet: Approximately 1 ng of P. islandicum DNA and 100 pmol of each primer were added to a PCR mixture containing 200 μM deoxyribonucleoside triphosphates and 2 U of Expand High Fidelity enzyme (Roche Diagnostics).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker