pcr mixture Search Results


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  • 99
    Thermo Fisher polymerase chain reaction pcr polymerase ready mix
    Polymerase Chain Reaction Pcr Polymerase Ready Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore redtaq readymix polymerase chain reaction pcr reaction
    Redtaq Readymix Polymerase Chain Reaction Pcr Reaction, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meridian Life Science sensimix pcr mix
    Sensimix Pcr Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 82/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche polymerase chain reaction pcr mixture
    Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time <t>PCR</t> and the DNA-binding dye, <t>SYBR</t> green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).
    Polymerase Chain Reaction Pcr Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kaneka Corp standard polymerase chain reaction pcr mix
    Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time <t>PCR</t> and the DNA-binding dye, <t>SYBR</t> green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).
    Standard Polymerase Chain Reaction Pcr Mix, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer polymerase chain reaction pcr mixture
    Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time <t>PCR</t> and the DNA-binding dye, <t>SYBR</t> green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).
    Polymerase Chain Reaction Pcr Mixture, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 82/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybrgreen polymerase chain reaction pcr mix
    Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time <t>PCR</t> and the DNA-binding dye, <t>SYBR</t> green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).
    Sybrgreen Polymerase Chain Reaction Pcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sangon Biotech polymerase chain reaction pcr mix kit
    Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time <t>PCR</t> and the DNA-binding dye, <t>SYBR</t> green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).
    Polymerase Chain Reaction Pcr Mix Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare polymerase chain reaction pcr nucleotide mix
    Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time <t>PCR</t> and the DNA-binding dye, <t>SYBR</t> green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).
    Polymerase Chain Reaction Pcr Nucleotide Mix, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr mixture
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co polymerase chain reaction pcr mixture
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Mixture, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kaneka Corp polymerase chain reaction pcr master mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Master Mix, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr master mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr pcr polymerase chain reaction master mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Sybr Pcr Polymerase Chain Reaction Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polymerase chain reaction pcr reaction mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Reaction Mix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman universal polymerase chain reaction pcr mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Taqman Universal Polymerase Chain Reaction Pcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green pcr master mixture
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Sybr Green Pcr Master Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega polymerase chain reaction pcr master mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meridian Life Science pcr mixture
    ( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: <t>cDNA</t> as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative <t>PCR</t> control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).
    Pcr Mixture, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 99/100, based on 481 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman universal pcr master mixture
    PRMT5 overexpression in lung cancer is evident at mRNA and protein levels. (a) There is a 6.13-fold increase in PRMT5 mRNA levels in lung tumors (LT), 8 cases, over matched nonneoplastic pulmonary parenchyma (L), as evident by <t>TaqMan</t> real time polymerase chain reaction <t>(PCR).</t> (b) PRMT5 and its symmetric methylation mark H4R3 are detected in lung carcinoma cell lines (NCI-A549, NCI-H520) but not in the nonneoplastic human pulmonary alveolar (HPAEpiC) and bronchial epithelial cell lines (HBEpiC); cellular localization experiments highlight nuclear and cytoplasmic fractions of PRMT5; Western immunoblot (b, c) ; immunohistochemistry (d) , NCI-H69, original magnification ×600.
    Taqman Universal Pcr Master Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Arraystar polymerase chain reaction pcr master mix
    PRMT5 overexpression in lung cancer is evident at mRNA and protein levels. (a) There is a 6.13-fold increase in PRMT5 mRNA levels in lung tumors (LT), 8 cases, over matched nonneoplastic pulmonary parenchyma (L), as evident by <t>TaqMan</t> real time polymerase chain reaction <t>(PCR).</t> (b) PRMT5 and its symmetric methylation mark H4R3 are detected in lung carcinoma cell lines (NCI-A549, NCI-H520) but not in the nonneoplastic human pulmonary alveolar (HPAEpiC) and bronchial epithelial cell lines (HBEpiC); cellular localization experiments highlight nuclear and cytoplasmic fractions of PRMT5; Western immunoblot (b, c) ; immunohistochemistry (d) , NCI-H69, original magnification ×600.
    Polymerase Chain Reaction Pcr Master Mix, supplied by Arraystar, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene quantitative polymerase chain reaction master mix
    PRMT5 overexpression in lung cancer is evident at mRNA and protein levels. (a) There is a 6.13-fold increase in PRMT5 mRNA levels in lung tumors (LT), 8 cases, over matched nonneoplastic pulmonary parenchyma (L), as evident by <t>TaqMan</t> real time polymerase chain reaction <t>(PCR).</t> (b) PRMT5 and its symmetric methylation mark H4R3 are detected in lung carcinoma cell lines (NCI-A549, NCI-H520) but not in the nonneoplastic human pulmonary alveolar (HPAEpiC) and bronchial epithelial cell lines (HBEpiC); cellular localization experiments highlight nuclear and cytoplasmic fractions of PRMT5; Western immunoblot (b, c) ; immunohistochemistry (d) , NCI-H69, original magnification ×600.
    Quantitative Polymerase Chain Reaction Master Mix, supplied by Stratagene, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction pcr master mix
    PRMT5 overexpression in lung cancer is evident at mRNA and protein levels. (a) There is a 6.13-fold increase in PRMT5 mRNA levels in lung tumors (LT), 8 cases, over matched nonneoplastic pulmonary parenchyma (L), as evident by <t>TaqMan</t> real time polymerase chain reaction <t>(PCR).</t> (b) PRMT5 and its symmetric methylation mark H4R3 are detected in lung carcinoma cell lines (NCI-A549, NCI-H520) but not in the nonneoplastic human pulmonary alveolar (HPAEpiC) and bronchial epithelial cell lines (HBEpiC); cellular localization experiments highlight nuclear and cytoplasmic fractions of PRMT5; Western immunoblot (b, c) ; immunohistochemistry (d) , NCI-H69, original magnification ×600.
    Polymerase Chain Reaction Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems polymerase chain reaction pcr mix
    PRMT5 overexpression in lung cancer is evident at mRNA and protein levels. (a) There is a 6.13-fold increase in PRMT5 mRNA levels in lung tumors (LT), 8 cases, over matched nonneoplastic pulmonary parenchyma (L), as evident by <t>TaqMan</t> real time polymerase chain reaction <t>(PCR).</t> (b) PRMT5 and its symmetric methylation mark H4R3 are detected in lung carcinoma cell lines (NCI-A549, NCI-H520) but not in the nonneoplastic human pulmonary alveolar (HPAEpiC) and bronchial epithelial cell lines (HBEpiC); cellular localization experiments highlight nuclear and cytoplasmic fractions of PRMT5; Western immunoblot (b, c) ; immunohistochemistry (d) , NCI-H69, original magnification ×600.
    Polymerase Chain Reaction Pcr Mix, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polymerase chain reaction pcr mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Polymerase Chain Reaction Pcr Mix, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad l polymerase chain reaction pcr mixture
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    L Polymerase Chain Reaction Pcr Mixture, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybrgreen polymerase chain reaction pcr master mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Sybrgreen Polymerase Chain Reaction Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Quantitative Polymerase Chain Reaction Master Mix, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
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    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
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    Image Search Results


    Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).

    Journal: Molecular Vision

    Article Title: Expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia

    doi:

    Figure Lengend Snippet: Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).

    Article Snippet: Total RNA (0.5 μg) was reverse transcribed as described above, and amplifications were performed using a commercial PCR mix incorporating SYBR green 1 (FastStart DNA Master Mix; Roche).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Binding Assay, SYBR Green Assay

    Regulation of retinal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew retina using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=6).

    Journal: Molecular Vision

    Article Title: Expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia

    doi:

    Figure Lengend Snippet: Regulation of retinal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew retina using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=6).

    Article Snippet: Total RNA (0.5 μg) was reverse transcribed as described above, and amplifications were performed using a commercial PCR mix incorporating SYBR green 1 (FastStart DNA Master Mix; Roche).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Binding Assay, SYBR Green Assay

    Regulation of scleral muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew sclera using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 5 day myopia data, 1 day myopia and normal data, n=6).

    Journal: Molecular Vision

    Article Title: Expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia

    doi:

    Figure Lengend Snippet: Regulation of scleral muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew sclera using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 5 day myopia data, 1 day myopia and normal data, n=6).

    Article Snippet: Total RNA (0.5 μg) was reverse transcribed as described above, and amplifications were performed using a commercial PCR mix incorporating SYBR green 1 (FastStart DNA Master Mix; Roche).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Binding Assay, SYBR Green Assay

    Schematic sketch of the CMV-specific IC (A) and CMV-specific PCR product (B). The IC DNA fragment consists of the CMV-specific primer sequences flanking the 5′ and 3′ termini of the neo gene sequence, which was used as heterologous DNA. IC DNA and CMV-specific DNA were amplified with the same set of primers. The sizes of the IC-specific and CMV-specific PCR products were 325 and 278 bp, respectively. The 3′ FRET hybridization probe specific for neo was labeled with LC-Red 640 and detected on channel F2 of the LightCycler instrument, whereas the 3′ FRET hybridization probe specific for CMV was labeled with LC-Red 705 and detected on channel F3.

    Journal: Journal of Clinical Microbiology

    Article Title: Normalized Quantification of Human Cytomegalovirus DNA by Competitive Real-Time PCR on the LightCycler Instrument

    doi: 10.1128/JCM.40.12.4547-4553.2002

    Figure Lengend Snippet: Schematic sketch of the CMV-specific IC (A) and CMV-specific PCR product (B). The IC DNA fragment consists of the CMV-specific primer sequences flanking the 5′ and 3′ termini of the neo gene sequence, which was used as heterologous DNA. IC DNA and CMV-specific DNA were amplified with the same set of primers. The sizes of the IC-specific and CMV-specific PCR products were 325 and 278 bp, respectively. The 3′ FRET hybridization probe specific for neo was labeled with LC-Red 640 and detected on channel F2 of the LightCycler instrument, whereas the 3′ FRET hybridization probe specific for CMV was labeled with LC-Red 705 and detected on channel F3.

    Article Snippet: The PCR mixtures were prepared by using LightCycler Fast Start DNA master hybridization probes (Roche Applied Science) supplemented with MgCl2 (4 mM), primers (0.4 μM each), and probes (0.2 μM each).

    Techniques: Polymerase Chain Reaction, Sequencing, Amplification, Hybridization, Labeling

    Normalized quantification of CMV DNA in PCR-inhibited samples by NQC-LC-PCR. Approximately 100 copies of CMV DNA and 100 copies of IC DNA were amplified in the presence of hemoglobin added in graded amounts. Quantification was performed in normalized fashion (open squares) and by the conventional real-time LightCycler method (closed squares). Quantified CMV copies in samples with hemoglobin were compared with quantified CMV copies in samples without hemoglobin by Student's t test. Symbols marked with an asterisk indicate significantly different CMV copy numbers ( P

    Journal: Journal of Clinical Microbiology

    Article Title: Normalized Quantification of Human Cytomegalovirus DNA by Competitive Real-Time PCR on the LightCycler Instrument

    doi: 10.1128/JCM.40.12.4547-4553.2002

    Figure Lengend Snippet: Normalized quantification of CMV DNA in PCR-inhibited samples by NQC-LC-PCR. Approximately 100 copies of CMV DNA and 100 copies of IC DNA were amplified in the presence of hemoglobin added in graded amounts. Quantification was performed in normalized fashion (open squares) and by the conventional real-time LightCycler method (closed squares). Quantified CMV copies in samples with hemoglobin were compared with quantified CMV copies in samples without hemoglobin by Student's t test. Symbols marked with an asterisk indicate significantly different CMV copy numbers ( P

    Article Snippet: The PCR mixtures were prepared by using LightCycler Fast Start DNA master hybridization probes (Roche Applied Science) supplemented with MgCl2 (4 mM), primers (0.4 μM each), and probes (0.2 μM each).

    Techniques: Polymerase Chain Reaction, Amplification

    Normalized quantification of CMV reference standard DNA. Ten, 20, 100, 200, and 1,000 copies of CMV reference standard DNA per capillary ( n = 8) were assessed by NQC-LC-PCR and conventional LightCycler PCR. (A) CMV copies measured by NQC-LC-PCR were plotted against applied CMV copies ( r = 0.972; P

    Journal: Journal of Clinical Microbiology

    Article Title: Normalized Quantification of Human Cytomegalovirus DNA by Competitive Real-Time PCR on the LightCycler Instrument

    doi: 10.1128/JCM.40.12.4547-4553.2002

    Figure Lengend Snippet: Normalized quantification of CMV reference standard DNA. Ten, 20, 100, 200, and 1,000 copies of CMV reference standard DNA per capillary ( n = 8) were assessed by NQC-LC-PCR and conventional LightCycler PCR. (A) CMV copies measured by NQC-LC-PCR were plotted against applied CMV copies ( r = 0.972; P

    Article Snippet: The PCR mixtures were prepared by using LightCycler Fast Start DNA master hybridization probes (Roche Applied Science) supplemented with MgCl2 (4 mM), primers (0.4 μM each), and probes (0.2 μM each).

    Techniques: Polymerase Chain Reaction

    Correlation between quantitative CMV DNA values from clinical samples analyzed by NQC-LC-PCR assay and by conventional LightCycler assay. The scatter diagram and the regression line show the relation of mean numbers of CMV DNA copies (squares) for 28 CMV-positive clinical samples assessed by both methods ( r = 0.973; P

    Journal: Journal of Clinical Microbiology

    Article Title: Normalized Quantification of Human Cytomegalovirus DNA by Competitive Real-Time PCR on the LightCycler Instrument

    doi: 10.1128/JCM.40.12.4547-4553.2002

    Figure Lengend Snippet: Correlation between quantitative CMV DNA values from clinical samples analyzed by NQC-LC-PCR assay and by conventional LightCycler assay. The scatter diagram and the regression line show the relation of mean numbers of CMV DNA copies (squares) for 28 CMV-positive clinical samples assessed by both methods ( r = 0.973; P

    Article Snippet: The PCR mixtures were prepared by using LightCycler Fast Start DNA master hybridization probes (Roche Applied Science) supplemented with MgCl2 (4 mM), primers (0.4 μM each), and probes (0.2 μM each).

    Techniques: Polymerase Chain Reaction

    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control PCR was performed under the same conditions except for without template DNA (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

    Journal: BioMed Research International

    Article Title: Population Abundance of Potentially Pathogenic Organisms in Intestinal Microbiome of Jungle Crow (Corvus macrorhynchos) Shown with 16S rRNA Gene-Based Microbial Community Analysis

    doi: 10.1155/2013/438956

    Figure Lengend Snippet: Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control PCR was performed under the same conditions except for without template DNA (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

    Article Snippet: PCR Conditions Twenty-five to fifty nanograms of isolated DNA were transferred to a PCR tube containing 50 μ L of a polymerase chain reaction (PCR) mixture containing LA Taq DNA polymerase (TaKaRa Bio, Shiga, Japan).

    Techniques: Amplification, Polymerase Chain Reaction, Marker

    miR-92a-3p downregulates NF2 mRNA and protein expression by interacting with its conserved MRE within the NF2 −3′UTR. (A) Semi-quantitative reverse transcription-polymerase chain reaction (semi-RT-qPCR), (B) quantitative RT-PCR (RT-qPCR) and (C) western blotting detection of NF2 /Merlin expression levels in HCT116 cells transfected with empty vector or miR-92a expression construct. (D) RT-qPCR and (E) western blotting detection of NF2 /Merlin expression in HCT116 cells co-transfected with pmR-ZsGreen1 and negative control target protector (TPneg), or with miR-92a expression construct and miR-92a-target protector (TP-92a). All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.

    Journal: Oncology Reports

    Article Title: MicroRNA-92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells

    doi: 10.3892/or.2019.7020

    Figure Lengend Snippet: miR-92a-3p downregulates NF2 mRNA and protein expression by interacting with its conserved MRE within the NF2 −3′UTR. (A) Semi-quantitative reverse transcription-polymerase chain reaction (semi-RT-qPCR), (B) quantitative RT-PCR (RT-qPCR) and (C) western blotting detection of NF2 /Merlin expression levels in HCT116 cells transfected with empty vector or miR-92a expression construct. (D) RT-qPCR and (E) western blotting detection of NF2 /Merlin expression in HCT116 cells co-transfected with pmR-ZsGreen1 and negative control target protector (TPneg), or with miR-92a expression construct and miR-92a-target protector (TP-92a). All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.

    Article Snippet: The 3′UTR of human NF2 isoform I (NM_000268.3) and the pre-miR-92a-1 gene (NR_029508.1) were amplified in a polymerase chain reaction (PCR) reaction mixture containing a final concentration of 1X PCR buffer (Titanium® Taq PCR buffer; Clontech Laboratories, Inc., Mountain View, CA, USA), 0.125 µM of each deoxynucleoside triphosphate (dNTPs) (Promega Corporation, Madison, WI, USA), 2 µM each of the forward and reverse primers, 1X Taq polymerase (Titanium® Taq polymerase; Clontech Laboratories, Inc.) and wild-type human genomic DNA template available in the Disease Molecular Biology and Epigenetics Laboratory of the National Institute of Molecular Biology and Biotechnology (DMBEL-NIMBB).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Transfection, Plasmid Preparation, Construct, Negative Control, Standard Deviation

    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by SYBR-Green I real-time PCR. The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.

    Journal: Oncology Letters

    Article Title: Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer

    doi: 10.3892/ol.2012.714

    Figure Lengend Snippet: Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by SYBR-Green I real-time PCR. The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.

    Article Snippet: The 25 μl PCR reaction mixture included 1× SYBR premix Ex Taq mix (Takara), 2 μl RT products and 10 nM of each forward and reverse primer.

    Techniques: Plasmid Preparation, SYBR Green Assay, Real-time Polymerase Chain Reaction, Produced

    The expression of the S. sq VEGFR gene in the H. roretzi tissues is correlated with the existence of circulatory system. ( a ) RT-PCR analysis of S. sq VEGFR gene expression in the different H. roretzi tissues. Upper panel: PCR was performed using a S. sq VEGFR -specific primer set and reverse-transcribed cDNA from the total RNA of each tissue as the template. Lower panel: PCR was preformed using the H. roretzi β-actin-specific primer set and reverse-transcribed cDNA from total RNA of each tissue as the template (positive control); ( b ) Anatomical scheme of H. roretzi .

    Journal: International Journal of Molecular Sciences

    Article Title: Vascular Endothelial Growth Factor Receptor Family in Ascidians, Halocynthia roretzi (Sea Squirt). Its High Expression in Circulatory System-Containing Tissues

    doi: 10.3390/ijms14034841

    Figure Lengend Snippet: The expression of the S. sq VEGFR gene in the H. roretzi tissues is correlated with the existence of circulatory system. ( a ) RT-PCR analysis of S. sq VEGFR gene expression in the different H. roretzi tissues. Upper panel: PCR was performed using a S. sq VEGFR -specific primer set and reverse-transcribed cDNA from the total RNA of each tissue as the template. Lower panel: PCR was preformed using the H. roretzi β-actin-specific primer set and reverse-transcribed cDNA from total RNA of each tissue as the template (positive control); ( b ) Anatomical scheme of H. roretzi .

    Article Snippet: The PCR mixture contained reverse-transcribed DNA from the total RNA samples of ascidian tissues, Fw primer (50 pmol), Rw primer (50 pmol), dNTPs (20 pmol), PCR buffer, and rTaq DNA polymerase (TaKaRa) in 100 μL.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Positive Control

    ( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: cDNA as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative PCR control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).

    Journal: Open Biology

    Article Title: The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering

    doi: 10.1098/rsob.160212

    Figure Lengend Snippet: ( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: cDNA as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative PCR control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).

    Article Snippet: For each sample, 2 µl of reverse transcribed cDNA was used as a template in a 25 µl total volume PCR mixture and amplified using MyTaq master mix (Bioline, UK) using the following cycling conditions: 94°C/30 s, followed by 35 cycles of 94°C/10 s, 53°C/10 s, 72°C/10 s and a final single 72°C/30 s cycle using the primers listed in electronic supplementary material, table S2.

    Techniques: Positive Control, Polymerase Chain Reaction

    PRMT5 overexpression in lung cancer is evident at mRNA and protein levels. (a) There is a 6.13-fold increase in PRMT5 mRNA levels in lung tumors (LT), 8 cases, over matched nonneoplastic pulmonary parenchyma (L), as evident by TaqMan real time polymerase chain reaction (PCR). (b) PRMT5 and its symmetric methylation mark H4R3 are detected in lung carcinoma cell lines (NCI-A549, NCI-H520) but not in the nonneoplastic human pulmonary alveolar (HPAEpiC) and bronchial epithelial cell lines (HBEpiC); cellular localization experiments highlight nuclear and cytoplasmic fractions of PRMT5; Western immunoblot (b, c) ; immunohistochemistry (d) , NCI-H69, original magnification ×600.

    Journal: Diagnostic Pathology

    Article Title: Cellular localization of protein arginine methyltransferase-5 correlates with grade of lung tumors

    doi: 10.1186/1746-1596-8-201

    Figure Lengend Snippet: PRMT5 overexpression in lung cancer is evident at mRNA and protein levels. (a) There is a 6.13-fold increase in PRMT5 mRNA levels in lung tumors (LT), 8 cases, over matched nonneoplastic pulmonary parenchyma (L), as evident by TaqMan real time polymerase chain reaction (PCR). (b) PRMT5 and its symmetric methylation mark H4R3 are detected in lung carcinoma cell lines (NCI-A549, NCI-H520) but not in the nonneoplastic human pulmonary alveolar (HPAEpiC) and bronchial epithelial cell lines (HBEpiC); cellular localization experiments highlight nuclear and cytoplasmic fractions of PRMT5; Western immunoblot (b, c) ; immunohistochemistry (d) , NCI-H69, original magnification ×600.

    Article Snippet: The PCR amplification was conducted in 25 μl reaction using the TaqMan Universal PCR Master Mixture (Applied Biosystems, Foster City, CA) according to the protocol supplied by the manufacturer.

    Techniques: Over Expression, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Methylation, Western Blot, Immunohistochemistry

    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by PCR using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M DNA marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Immortalized mouse dental papilla mesenchymal cells preserve odontoblastic phenotype and respond to bone morphogenetic protein 2

    doi: 10.1007/s11626-013-9641-1

    Figure Lengend Snippet: Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by PCR using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M DNA marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.

    Article Snippet: Two-hundred nanograms of DNA (for pSV3 neo plasmid DNA 10 ng) were diluted in a 25-μl polymerase chain reaction (PCR) mix (Sigma–Aldrich).

    Techniques: Expressing, Amplification, Polymerase Chain Reaction, Staining, Marker, Negative Control, Immunohistochemistry, Western Blot, SDS Page

    Identification of SV40 transformation. ( A ) Genomic DNA in the primary and immortalized dental papilla mesenchymal cells was isolated and amplified by the SV40 specific primer. PCR products were run on an agarose gel and stained with ethidium bromide. Lane 1 , DNA marker (Low DNA mass ladder, Invitrogen); lane 2 , control; lane 3 , primary dental papilla mesenchymal cells; lane 4 , immortalized cells. ( B , C ) Immunohistochemical staining with antibody against simian virus 40 large T-antigen (SV40 T-Ag) in iMDP-3 cells and immunolabeling of SV40 protein was mostly present in the nucleus ( green color ; B ) whereas immunostaining was not seen in the primary cells ( C ). The cells were stained with DAPI for the nucleus. Con control, Pri primary, Imm immortalized.

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Immortalized mouse dental papilla mesenchymal cells preserve odontoblastic phenotype and respond to bone morphogenetic protein 2

    doi: 10.1007/s11626-013-9641-1

    Figure Lengend Snippet: Identification of SV40 transformation. ( A ) Genomic DNA in the primary and immortalized dental papilla mesenchymal cells was isolated and amplified by the SV40 specific primer. PCR products were run on an agarose gel and stained with ethidium bromide. Lane 1 , DNA marker (Low DNA mass ladder, Invitrogen); lane 2 , control; lane 3 , primary dental papilla mesenchymal cells; lane 4 , immortalized cells. ( B , C ) Immunohistochemical staining with antibody against simian virus 40 large T-antigen (SV40 T-Ag) in iMDP-3 cells and immunolabeling of SV40 protein was mostly present in the nucleus ( green color ; B ) whereas immunostaining was not seen in the primary cells ( C ). The cells were stained with DAPI for the nucleus. Con control, Pri primary, Imm immortalized.

    Article Snippet: Two-hundred nanograms of DNA (for pSV3 neo plasmid DNA 10 ng) were diluted in a 25-μl polymerase chain reaction (PCR) mix (Sigma–Aldrich).

    Techniques: Transformation Assay, Isolation, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Marker, Immunohistochemistry, Immunolabeling, Immunostaining