Journal: PLoS ONE
Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction
Figure Lengend Snippet: Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
Article Snippet: Three commercial PCR master mixes, including the AccuPower PCR PreMix (Bioneer, Daejeon, Korea), AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, CA, USA), and HotStarTaq Plus Master Mix (Qiagen, Valencia, CA, USA), were used for the evaluation of the UCNPs effects on the PCR.
Techniques: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Marker