pcr mix Thermo Fisher Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher express qrt pcr supermix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Express Qrt Pcr Supermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/express qrt pcr supermix/product/Thermo Fisher
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    express qrt pcr supermix - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    86
    Thermo Fisher sybrgreen polymerase chain reaction pcr mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Sybrgreen Polymerase Chain Reaction Pcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybrgreen polymerase chain reaction pcr mix/product/Thermo Fisher
    Average 86 stars, based on 170 article reviews
    Price from $9.99 to $1999.99
    sybrgreen polymerase chain reaction pcr mix - by Bioz Stars, 2020-02
    86/100 stars
      Buy from Supplier

    78
    Thermo Fisher sybr pcr polymerase chain reaction master mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Sybr Pcr Polymerase Chain Reaction Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr pcr polymerase chain reaction master mix/product/Thermo Fisher
    Average 78 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    sybr pcr polymerase chain reaction master mix - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    81
    Thermo Fisher taqman universal polymerase chain reaction pcr mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Taqman Universal Polymerase Chain Reaction Pcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman universal polymerase chain reaction pcr mix/product/Thermo Fisher
    Average 81 stars, based on 140 article reviews
    Price from $9.99 to $1999.99
    taqman universal polymerase chain reaction pcr mix - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

    95
    Thermo Fisher sybr green polymerase chain reaction master mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Sybr Green Polymerase Chain Reaction Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green polymerase chain reaction master mix/product/Thermo Fisher
    Average 95 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    sybr green polymerase chain reaction master mix - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    93
    Thermo Fisher taqman universal polymerase chain reaction master mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Taqman Universal Polymerase Chain Reaction Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman universal polymerase chain reaction master mix/product/Thermo Fisher
    Average 93 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    taqman universal polymerase chain reaction master mix - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    86
    Thermo Fisher polymerase chain reaction pcr master mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Polymerase Chain Reaction Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr master mix/product/Thermo Fisher
    Average 86 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr master mix - by Bioz Stars, 2020-02
    86/100 stars
      Buy from Supplier

    91
    Thermo Fisher polymerase chain reaction qpcr analysis
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Polymerase Chain Reaction Qpcr Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction qpcr analysis/product/Thermo Fisher
    Average 91 stars, based on 73 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction qpcr analysis - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    78
    Thermo Fisher taqman one step polymerase chain reaction master mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Taqman One Step Polymerase Chain Reaction Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman one step polymerase chain reaction master mix/product/Thermo Fisher
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    taqman one step polymerase chain reaction master mix - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    77
    Thermo Fisher real time polymerase chain reaction master mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Real Time Polymerase Chain Reaction Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time polymerase chain reaction master mix/product/Thermo Fisher
    Average 77 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    real time polymerase chain reaction master mix - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    77
    Thermo Fisher polymerase chain reaction pcr enzyme mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Polymerase Chain Reaction Pcr Enzyme Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr enzyme mix/product/Thermo Fisher
    Average 77 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr enzyme mix - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    90
    Thermo Fisher sybrgreen polymerase chain reaction pcr master mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Sybrgreen Polymerase Chain Reaction Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybrgreen polymerase chain reaction pcr master mix/product/Thermo Fisher
    Average 90 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    sybrgreen polymerase chain reaction pcr master mix - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    79
    Thermo Fisher gene expression polymerase chain reaction mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Gene Expression Polymerase Chain Reaction Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene expression polymerase chain reaction mix/product/Thermo Fisher
    Average 79 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    gene expression polymerase chain reaction mix - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    77
    Thermo Fisher sybr green quantitative polymerase chain reaction qpcr master mix
    Validation of RNAseq data by <t>qRT-PCR.</t> ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).
    Sybr Green Quantitative Polymerase Chain Reaction Qpcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green quantitative polymerase chain reaction qpcr master mix/product/Thermo Fisher
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    sybr green quantitative polymerase chain reaction qpcr master mix - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    90
    Thermo Fisher taq dna polymerase
    Differential peak morphologies of androgen receptor alleles resulting from <t>DNA</t> dilution and reagent use. Lynx positive control DNA sample amplified with Invitrogen <t>Taq</t> DNA Polymerase and diluted to 1:10 ( a ), 1:20 ( b ), and 1:50 ( c ) ratios with deionized water. Lynx positive control DNA sample amplified with Invitrogen Platinum Taq DNA Polymerase (no dilution necessary) ( d )
    Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 33236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/Thermo Fisher
    Average 90 stars, based on 33236 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    84
    Thermo Fisher transcription quantitative polymerase chain reaction rt qpcr master mix
    Differential peak morphologies of androgen receptor alleles resulting from <t>DNA</t> dilution and reagent use. Lynx positive control DNA sample amplified with Invitrogen <t>Taq</t> DNA Polymerase and diluted to 1:10 ( a ), 1:20 ( b ), and 1:50 ( c ) ratios with deionized water. Lynx positive control DNA sample amplified with Invitrogen Platinum Taq DNA Polymerase (no dilution necessary) ( d )
    Transcription Quantitative Polymerase Chain Reaction Rt Qpcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcription quantitative polymerase chain reaction rt qpcr master mix/product/Thermo Fisher
    Average 84 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    transcription quantitative polymerase chain reaction rt qpcr master mix - by Bioz Stars, 2020-02
    84/100 stars
      Buy from Supplier

    99
    Thermo Fisher real time polymerase chain reaction pcr
    Differential peak morphologies of androgen receptor alleles resulting from <t>DNA</t> dilution and reagent use. Lynx positive control DNA sample amplified with Invitrogen <t>Taq</t> DNA Polymerase and diluted to 1:10 ( a ), 1:20 ( b ), and 1:50 ( c ) ratios with deionized water. Lynx positive control DNA sample amplified with Invitrogen Platinum Taq DNA Polymerase (no dilution necessary) ( d )
    Real Time Polymerase Chain Reaction Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time polymerase chain reaction pcr/product/Thermo Fisher
    Average 99 stars, based on 4207 article reviews
    Price from $9.99 to $1999.99
    real time polymerase chain reaction pcr - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    76
    Thermo Fisher quantitative polymerase chain reaction q pcr mixtures
    Differential peak morphologies of androgen receptor alleles resulting from <t>DNA</t> dilution and reagent use. Lynx positive control DNA sample amplified with Invitrogen <t>Taq</t> DNA Polymerase and diluted to 1:10 ( a ), 1:20 ( b ), and 1:50 ( c ) ratios with deionized water. Lynx positive control DNA sample amplified with Invitrogen Platinum Taq DNA Polymerase (no dilution necessary) ( d )
    Quantitative Polymerase Chain Reaction Q Pcr Mixtures, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative polymerase chain reaction q pcr mixtures/product/Thermo Fisher
    Average 76 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    quantitative polymerase chain reaction q pcr mixtures - by Bioz Stars, 2020-02
    76/100 stars
      Buy from Supplier

    75
    Thermo Fisher rnase dnase free polymerase chain reaction pcr grade water
    Differential peak morphologies of androgen receptor alleles resulting from <t>DNA</t> dilution and reagent use. Lynx positive control DNA sample amplified with Invitrogen <t>Taq</t> DNA Polymerase and diluted to 1:10 ( a ), 1:20 ( b ), and 1:50 ( c ) ratios with deionized water. Lynx positive control DNA sample amplified with Invitrogen Platinum Taq DNA Polymerase (no dilution necessary) ( d )
    Rnase Dnase Free Polymerase Chain Reaction Pcr Grade Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase dnase free polymerase chain reaction pcr grade water/product/Thermo Fisher
    Average 75 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase dnase free polymerase chain reaction pcr grade water - by Bioz Stars, 2020-02
    75/100 stars
      Buy from Supplier

    90
    Thermo Fisher amplitaq gold 360 master mix
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) <t>AmpliTaq</t> <t>Gold</t> 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Amplitaq Gold 360 Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1468 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold 360 master mix/product/Thermo Fisher
    Average 90 stars, based on 1468 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold 360 master mix - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    77
    Thermo Fisher quantitative polymerase chain reaction rox universal genotyping master mix
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) <t>AmpliTaq</t> <t>Gold</t> 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Quantitative Polymerase Chain Reaction Rox Universal Genotyping Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative polymerase chain reaction rox universal genotyping master mix/product/Thermo Fisher
    Average 77 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    quantitative polymerase chain reaction rox universal genotyping master mix - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    90
    Thermo Fisher fast sybr green master mix
    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with <t>SYBR</t> ™ Safe DNA Gel Stain in agarose gels. (C) <t>qRT-PCR</t> was performed to confirm the results in (B).
    Fast Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 17170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast sybr green master mix/product/Thermo Fisher
    Average 90 stars, based on 17170 article reviews
    Price from $9.99 to $1999.99
    fast sybr green master mix - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    78
    Thermo Fisher sybr green real time quantitative polymerase chain reaction qpcr mix
    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with <t>SYBR</t> ™ Safe DNA Gel Stain in agarose gels. (C) <t>qRT-PCR</t> was performed to confirm the results in (B).
    Sybr Green Real Time Quantitative Polymerase Chain Reaction Qpcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green real time quantitative polymerase chain reaction qpcr mix/product/Thermo Fisher
    Average 78 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    sybr green real time quantitative polymerase chain reaction qpcr mix - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    90
    Thermo Fisher sybr green pcr master mix
    Comparison to TaqMan ® technology. Total RNA was extracted from isolated monocytes stimulated for 6 hours by LPS, serial-diluted 1:10 and retro-transcribed to generate standard curves by plotting the C T against the concentration of this cellular RNA in arbitrary units. TNF-α transcripts were amplified by real time <t>PCR</t> using either ( A ) the TaqMan ® commercial kit and the TaqMan ® universal PCR master mix or ( B ) our primers and the <t>SYBR</t> Green PCR master mix. Data represent the standard curves obtained for the two techniques, their slopes and the deducted efficiencies.
    Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 78482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green pcr master mix/product/Thermo Fisher
    Average 90 stars, based on 78482 article reviews
    Price from $9.99 to $1999.99
    sybr green pcr master mix - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher amplitaq gold dna polymerase
    Comparison to TaqMan ® technology. Total RNA was extracted from isolated monocytes stimulated for 6 hours by LPS, serial-diluted 1:10 and retro-transcribed to generate standard curves by plotting the C T against the concentration of this cellular RNA in arbitrary units. TNF-α transcripts were amplified by real time <t>PCR</t> using either ( A ) the TaqMan ® commercial kit and the TaqMan ® universal PCR master mix or ( B ) our primers and the <t>SYBR</t> Green PCR master mix. Data represent the standard curves obtained for the two techniques, their slopes and the deducted efficiencies.
    Amplitaq Gold Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/Thermo Fisher
    Average 90 stars, based on 10123 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher platinum pcr super mix high fidelity
    <t>RT-PCR</t> amplification of TRIM5α and TRIMCyp from Old World primate total RNA. Total RNA was isolated from Owl monkey kidney (OMK) cells, M. mulatta PBMC, M. fascicularis PBMC, and M. nemestrina PBMC. Primers were designed to amplify either TRIM5α (odd lanes) or TRIMCyp (even lanes). Lane L, kb+ ladder; lane 1, dH 2 O TRIM5α; lane 2, dH 2 O TRIMCyp; lane 3, OMK TRIM5α; lane 4, OMK TRIMCyp; lane 5, M. mulatta TRIM5α; lane 6, M. mulatta TRIMCyp; lane 7, M. fascicularis TRIM5α; lane 8, M. fascicularis TRIMCyp; lane 9, M. nemestrina <t>TRIM5;</t> and lane 10, M. nemestrina TRIMCyp.
    Platinum Pcr Super Mix High Fidelity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum pcr super mix high fidelity/product/Thermo Fisher
    Average 90 stars, based on 122 article reviews
    Price from $9.99 to $1999.99
    platinum pcr super mix high fidelity - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher taqman fast universal pcr master mix
    Editing of CEP290 Transcripts Occurs in HEK293T after Transfection with PTMs (A) Illustration of adeno-associated virus genome arrangement of a CEP290 5′ PTM with binding domain 07 (PTM_07) with either no poly-adenylation signal (NPA) or with a bovine growth hormone poly-adenylation signal (PA). ITR, inverted terminal repeat; CMV, cytomegalovirus promoter; PCDS, partial coding DNA sequence of CEP290 ; 5′ SS, 5′ splice site. (B) qPCR was performed on HEK293T transfected with a plasmid encoding GFP as a transfection control or the plasmids in (A). <t>TaqMan</t> probes were designed to the junctions indicated: CEP290 exons 26 and 27 (hEx26-hEx27), a region within the 5′ codon optimized partial coding DNA sequence (coPCDS), or the novel junction of 5′ coPCDS and endogenous Homo sapiens exon 27 to signify trans -splicing (coPCDS-hEx27). Significant variation within replicates for coPCDS-hEx27 was present because the Ct was crossed at 35 cycles with either PTM; however, no amplification was observed through 40 cycles in GFP-treated samples. Samples were standardized to β-2-microglobin and normalized to NPA. Error bars are relative quantity minimum and maximum 95% confidence intervals. (C) Agarose gel electrophoresis of one of the replicate reactions from (B). (D) Densitometry analysis of the bands in (C). (E) Sanger sequencing following TOPO-cloning of the <t>PCR</t> product comprising the coPCDS-hEx27 junction visualized in (C). Nucleotide differences between Homo sapiens and codon-optimized CEP290 are noted by asterisks. The junction between the 5′ coPCDS and endogenous exon 27 is marked by a vertical dashed line.
    Taqman Fast Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5522 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman fast universal pcr master mix/product/Thermo Fisher
    Average 90 stars, based on 5522 article reviews
    Price from $9.99 to $1999.99
    taqman fast universal pcr master mix - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher dreamtaq green pcr master mix
    Editing of CEP290 Transcripts Occurs in HEK293T after Transfection with PTMs (A) Illustration of adeno-associated virus genome arrangement of a CEP290 5′ PTM with binding domain 07 (PTM_07) with either no poly-adenylation signal (NPA) or with a bovine growth hormone poly-adenylation signal (PA). ITR, inverted terminal repeat; CMV, cytomegalovirus promoter; PCDS, partial coding DNA sequence of CEP290 ; 5′ SS, 5′ splice site. (B) qPCR was performed on HEK293T transfected with a plasmid encoding GFP as a transfection control or the plasmids in (A). <t>TaqMan</t> probes were designed to the junctions indicated: CEP290 exons 26 and 27 (hEx26-hEx27), a region within the 5′ codon optimized partial coding DNA sequence (coPCDS), or the novel junction of 5′ coPCDS and endogenous Homo sapiens exon 27 to signify trans -splicing (coPCDS-hEx27). Significant variation within replicates for coPCDS-hEx27 was present because the Ct was crossed at 35 cycles with either PTM; however, no amplification was observed through 40 cycles in GFP-treated samples. Samples were standardized to β-2-microglobin and normalized to NPA. Error bars are relative quantity minimum and maximum 95% confidence intervals. (C) Agarose gel electrophoresis of one of the replicate reactions from (B). (D) Densitometry analysis of the bands in (C). (E) Sanger sequencing following TOPO-cloning of the <t>PCR</t> product comprising the coPCDS-hEx27 junction visualized in (C). Nucleotide differences between Homo sapiens and codon-optimized CEP290 are noted by asterisks. The junction between the 5′ coPCDS and endogenous exon 27 is marked by a vertical dashed line.
    Dreamtaq Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dreamtaq green pcr master mix/product/Thermo Fisher
    Average 90 stars, based on 1216 article reviews
    Price from $9.99 to $1999.99
    dreamtaq green pcr master mix - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher power sybr green pcr master mix
    Effect of CIB1 knockdown on de novo KSHV infection. (A) Untransduced, control or CIB1-shRNA transduced HMVEC-d cells were infected with KSHV (20 DNA copies/cell). At 24 h p.i., cells were harvested, total RNA was isolated, and viral gene expression was determined by real-time <t>RT-PCR</t> with KSHV ORF73 gene specific primers. Results are presented as percentage of inhibition of KSHV gene expression by sh-CIB1 or control compared with the infected untransduced cells. ***P = 0.0001. (B) (i) Control or CIB1-shRNA transduced HMVEC-d cells were mock or KSHV infected (20 DNA copies/cell) for 2 h at 37°C, washed to remove unbound viruses, and continued to culture for another 46 h. At 48 h p.i., cells were processed for immunofluorescence analysis using mouse anti-LANA-1 antibodies and co-stained with DAPI. Representative images are shown. (B) (ii) The percentage of cells observed positive for characteristic punctate LANA-1 staining in IFA is presented graphically. A minimum of three independent fields, each with at least 10 cells were chosen. Error bars show ± SD. (C) Control or CIB1-shRNA transduced HMVEC-d cells were mock or HSV-1 infected (3 pfu/cell) for 2 h at 37°C, washed to remove unbound viruses, and incubated for another 6 h. At 8 h p.i., cells were harvested, total RNA was isolated, and HSV-1 gene expression was quantified by <t>SYBR</t> green q-PCR method with ICP0 and ICP4 gene specific primers. Results are presented as fold HSV-1 gene expression normalized to internal tubulin control. Error bars show ± SD. (D) 293 cells were either mock-transfected or transfected with CIB1 overexpressing vector pcDNA-CIB1-Myc using lipofectamine. At 48 h post-transfection, CIB1 overexpression was examined by Western blotting with rabbit anti-CIB1 and mouse anti-Myc antibodies. Actin was used as loading control. (E) At 48 h post-transfection, transfected 293 cells were infected with KSHV (20 DNA copies/cell) for 2 h at 37°C for entry experiments. Post-washing, total DNA was isolated and KSHV entry was determined by real-time DNA-PCR for the ORF73 gene. Each reaction was done in triplicate and each bar represents the average ± SD of three independent experiments. Results are presented as percentage increase in KSHV DNA internalization in pcDNA-CIB1-Myc expressing cells compared with the control vector transfected cells, which is considered as 100%.
    Power Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 53341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/power sybr green pcr master mix/product/Thermo Fisher
    Average 90 stars, based on 53341 article reviews
    Price from $9.99 to $1999.99
    power sybr green pcr master mix - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher taqman universal pcr master mix
    Correlation of array matrix data to quantitative real-time <t>PCR.</t> Labeled RNA samples were made from human and brain total RNA. These were hybridized to separate array matrices containing 633 human genes. Six technical replicates were included for each sample. Twenty-one genes from this list were selected for analysis by <t>TaqMan</t> quantitative real-time PCR. A scatter plot of hybridization intensities of the liver and brain samples on the array matrix is shown in A . Genes selected for further analysis are shaded orange. A scatter plot of log-transformed hybridization signal ratios as determined by the two methods is shown in B .
    Taqman Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 41432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman universal pcr master mix/product/Thermo Fisher
    Average 90 stars, based on 41432 article reviews
    Price from $9.99 to $1999.99
    taqman universal pcr master mix - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher maxima sybr green qpcr master mix
    An example of the <t>SYBR</t> Green <t>RD-qPCR</t> assay of 5-LOX DNA methylation (shown are graphs obtained from 10 cerebellar samples). The samples were digested with the methylation-sensitive endonucleases (AciI, BstUI, HinP1I, and HpaII) as described in Material and Methods. The PCR reaction for the promoter-5′UTR (10 samples each: AciI = blue, BstUI = red, HinP1I = green, and HpaII = gray) and corresponding input control regions (shown in yellow) were carried out in separate tubes. Panel A shows the dissociation curve data, which indicate the presence of only one PCR product (peak) for each specific set of primers (fluorescence (first derivative of the raw fluorescence reading multiplied by −1) on the Y-axis versus the PCR product melting temperature (°C) on the X-axis). Panel B shows examples of the amplification plots used for calculating the quantitative data (the amplification plots fluorescence (baseline-corrected raw fluorescence) on the Y-axis versus cycle number on the X-axis). In this assay, the threshold cycle is inversely proportional to the log of the initial copy number. In other words, the more template that is present initially, the fewer the number of cycles required for the fluorescence signal to be detectable above background.
    Maxima Sybr Green Qpcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maxima sybr green qpcr master mix/product/Thermo Fisher
    Average 90 stars, based on 4584 article reviews
    Price from $9.99 to $1999.99
    maxima sybr green qpcr master mix - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    Image Search Results


    Validation of RNAseq data by qRT-PCR. ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Transcriptome profiling of Plasmodium vivax in Saimiri monkeys identifies potential ligands for invasion

    doi: 10.1073/pnas.1818485116

    Figure Lengend Snippet: Validation of RNAseq data by qRT-PCR. ( A ) qRT-PCR was performed on eight genes from P. vivax Sal I infection in Saimiri and Aotus infection. The normalized mRNA level (averaged) and SD for all eight genes [PVX_099980 (MSP1), PVX_110810 (DBP1), PVX_094230 (hypothetical protein), PVX_01695 (Pf-fam-b), PVX_104690 (Pv-fam-c), PVX_097670 (MSP3g), PVX_092275 (AMA1), and PVX_112690 (Pv-fam-a)] were graphed for each Saimiri and Aotus monkey sample. Each gene signal was normalized to the housekeeping gene GST. Each bar represents an average of two monkeys. The error bar is based on the SD. ( B ) Table shows the correlation of qRT-PCR and the RNAseq data with the number of samples ( n = 4) and P value (significance of correlation).

    Article Snippet: Briefly, Life Technologies Express qRT-PCR Supermix with premixed ROX dye (ThermoFisher Scientific) was used to perform the assays.

    Techniques: Quantitative RT-PCR, Infection

    Differential peak morphologies of androgen receptor alleles resulting from DNA dilution and reagent use. Lynx positive control DNA sample amplified with Invitrogen Taq DNA Polymerase and diluted to 1:10 ( a ), 1:20 ( b ), and 1:50 ( c ) ratios with deionized water. Lynx positive control DNA sample amplified with Invitrogen Platinum Taq DNA Polymerase (no dilution necessary) ( d )

    Journal: BMC Genetics

    Article Title: A test of somatic mosaicism in the androgen receptor gene of Canada lynx (Lynx canadensis)

    doi: 10.1186/s12863-015-0284-y

    Figure Lengend Snippet: Differential peak morphologies of androgen receptor alleles resulting from DNA dilution and reagent use. Lynx positive control DNA sample amplified with Invitrogen Taq DNA Polymerase and diluted to 1:10 ( a ), 1:20 ( b ), and 1:50 ( c ) ratios with deionized water. Lynx positive control DNA sample amplified with Invitrogen Platinum Taq DNA Polymerase (no dilution necessary) ( d )

    Article Snippet: Amplification was conducted in a 10ul reaction containing deionized water (Invitrogen), 10X PCR Reaction Buffer (Invitrogen), 50 mM MgCl2 (Invitrogen), 100 mM dNTP solution (Invitrogen), 3 mg/mL BSA, 40uM forward and reverse primers (Integrated DNA Technologies) mentioned above (forward primers labeled with the fluorescent dye HEX), 0.0375U Invitrogen Taq DNA Polymerase, and 5 ng of DNA.

    Techniques: Positive Control, Amplification

    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Journal: PLoS ONE

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction

    doi: 10.1371/journal.pone.0073408

    Figure Lengend Snippet: Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Article Snippet: Three commercial PCR master mixes, including the AccuPower PCR PreMix (Bioneer, Daejeon, Korea), AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, CA, USA), and HotStarTaq Plus Master Mix (Qiagen, Valencia, CA, USA), were used for the evaluation of the UCNPs effects on the PCR.

    Techniques: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Marker

    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Journal: Heart rhythm

    Article Title: HuR-mediated SCN5A mRNA stability reduces arrhythmic risk in heart failure

    doi: 10.1016/j.hrthm.2018.02.018

    Figure Lengend Snippet: HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Article Snippet: Quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) was carried out using gene-specific primers, Fast SYBR® Green Master Mix and 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Isolation, Synthesized, Polymerase Chain Reaction, Staining, Quantitative RT-PCR

    Comparison to TaqMan ® technology. Total RNA was extracted from isolated monocytes stimulated for 6 hours by LPS, serial-diluted 1:10 and retro-transcribed to generate standard curves by plotting the C T against the concentration of this cellular RNA in arbitrary units. TNF-α transcripts were amplified by real time PCR using either ( A ) the TaqMan ® commercial kit and the TaqMan ® universal PCR master mix or ( B ) our primers and the SYBR Green PCR master mix. Data represent the standard curves obtained for the two techniques, their slopes and the deducted efficiencies.

    Journal: BMC Immunology

    Article Title: CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use

    doi: 10.1186/1471-2172-6-5

    Figure Lengend Snippet: Comparison to TaqMan ® technology. Total RNA was extracted from isolated monocytes stimulated for 6 hours by LPS, serial-diluted 1:10 and retro-transcribed to generate standard curves by plotting the C T against the concentration of this cellular RNA in arbitrary units. TNF-α transcripts were amplified by real time PCR using either ( A ) the TaqMan ® commercial kit and the TaqMan ® universal PCR master mix or ( B ) our primers and the SYBR Green PCR master mix. Data represent the standard curves obtained for the two techniques, their slopes and the deducted efficiencies.

    Article Snippet: An aliquot of 5 μL of the RT reaction was amplified in duplicate in a final volume of 30 μL of SYBR Green PCR Master mix (Applied Biosystems, Foster City, CA, USA).

    Techniques: Isolation, Concentration Assay, Amplification, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, SYBR Green Assay

    Primer validation. A. Agarose gel electrophoresis of several CyProQuant-PCR products generated by amplification of a pool of cDNA from PBMC stimulated in vitro by LPS or PHA. Scale is shown in base pairs (bp). B. Dissociation curve analysis of these CyProQuant-PCR products. Negative derivative of the fluorescence is plotted against temperature. The single peak shows that SYBR Green fluorescence detects only the specific CyProQuant-PCR product. C. Amplification plots and standard curve resulting from the amplification of a range of β2-MG external RNA standard using CyProQuant-PCR.

    Journal: BMC Immunology

    Article Title: CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use

    doi: 10.1186/1471-2172-6-5

    Figure Lengend Snippet: Primer validation. A. Agarose gel electrophoresis of several CyProQuant-PCR products generated by amplification of a pool of cDNA from PBMC stimulated in vitro by LPS or PHA. Scale is shown in base pairs (bp). B. Dissociation curve analysis of these CyProQuant-PCR products. Negative derivative of the fluorescence is plotted against temperature. The single peak shows that SYBR Green fluorescence detects only the specific CyProQuant-PCR product. C. Amplification plots and standard curve resulting from the amplification of a range of β2-MG external RNA standard using CyProQuant-PCR.

    Article Snippet: An aliquot of 5 μL of the RT reaction was amplified in duplicate in a final volume of 30 μL of SYBR Green PCR Master mix (Applied Biosystems, Foster City, CA, USA).

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Generated, Amplification, In Vitro, Fluorescence, SYBR Green Assay

    RT-PCR amplification of TRIM5α and TRIMCyp from Old World primate total RNA. Total RNA was isolated from Owl monkey kidney (OMK) cells, M. mulatta PBMC, M. fascicularis PBMC, and M. nemestrina PBMC. Primers were designed to amplify either TRIM5α (odd lanes) or TRIMCyp (even lanes). Lane L, kb+ ladder; lane 1, dH 2 O TRIM5α; lane 2, dH 2 O TRIMCyp; lane 3, OMK TRIM5α; lane 4, OMK TRIMCyp; lane 5, M. mulatta TRIM5α; lane 6, M. mulatta TRIMCyp; lane 7, M. fascicularis TRIM5α; lane 8, M. fascicularis TRIMCyp; lane 9, M. nemestrina TRIM5; and lane 10, M. nemestrina TRIMCyp.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: From the Cover: TRIMCyp expression in Old World primates Macaca nemestrina and Macaca fascicularis

    doi: 10.1073/pnas.0709511105

    Figure Lengend Snippet: RT-PCR amplification of TRIM5α and TRIMCyp from Old World primate total RNA. Total RNA was isolated from Owl monkey kidney (OMK) cells, M. mulatta PBMC, M. fascicularis PBMC, and M. nemestrina PBMC. Primers were designed to amplify either TRIM5α (odd lanes) or TRIMCyp (even lanes). Lane L, kb+ ladder; lane 1, dH 2 O TRIM5α; lane 2, dH 2 O TRIMCyp; lane 3, OMK TRIM5α; lane 4, OMK TRIMCyp; lane 5, M. mulatta TRIM5α; lane 6, M. mulatta TRIMCyp; lane 7, M. fascicularis TRIM5α; lane 8, M. fascicularis TRIMCyp; lane 9, M. nemestrina TRIM5; and lane 10, M. nemestrina TRIMCyp.

    Article Snippet: Primers MneT5Aex51.23 (5′-GGTGTGGATGGCATCATTAAAAG-3′) and CypA*RMCSNotI were used to amplify a fragment of the TRIM5 gene from PBMC genomic DNA with Platinum PCR Super Mix High Fidelity (error rate: 1.8 ± 0.4 × 10−6 ; Invitrogen).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Isolation

    Genomic characterization of Old World primate TRIMCyp. ( A ) PCR amplification of the TRIM5 gene from M. mulatta , M. nemestrina , and M. fascicularis PBMC genomic DNA using a forward primer specific for exon 6 and a reverse primer specific for exon 8. Lane 1, M. mulatta ; lane 2, M. fascicularis ; lane 3, M. nemestrina ; and lane 4, kb+ DNA ladder. ( B ) Sequence of the M. fascicularis TRIM5 gene from intron 6 to exon 8. The underlined sequence indicates the location corresponding to the site of CypA retrotransposition in A. trivirgatus . ( C ) PCR amplification of TRIM5 exon 8 and CypA. M. nemestrina and M. fascicularis PBMC genomic DNA was PCR-amplified with a forward primer specific for TRIM5 exon 5 and a reverse primer specific for CypA . Lane 1, kb+ DNA ladder; lane 2, dH 2 O; lane 3, M. fascicularis ; and lane 4, M. nemestrina . ( D ) Sequence of the M. fascicularis TRIM5 3′ UTR and retrotransposed CypA . Lowercase letters indicate the TRIM5 3′ UTR. Italicized and underlined letters identify the conserved retrotransposition sequence. Capital letters identify the retrotransposed CypA sequence in TRIMCyp. Bold letters highlight the start and stop codons of the CypA coding sequence.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: From the Cover: TRIMCyp expression in Old World primates Macaca nemestrina and Macaca fascicularis

    doi: 10.1073/pnas.0709511105

    Figure Lengend Snippet: Genomic characterization of Old World primate TRIMCyp. ( A ) PCR amplification of the TRIM5 gene from M. mulatta , M. nemestrina , and M. fascicularis PBMC genomic DNA using a forward primer specific for exon 6 and a reverse primer specific for exon 8. Lane 1, M. mulatta ; lane 2, M. fascicularis ; lane 3, M. nemestrina ; and lane 4, kb+ DNA ladder. ( B ) Sequence of the M. fascicularis TRIM5 gene from intron 6 to exon 8. The underlined sequence indicates the location corresponding to the site of CypA retrotransposition in A. trivirgatus . ( C ) PCR amplification of TRIM5 exon 8 and CypA. M. nemestrina and M. fascicularis PBMC genomic DNA was PCR-amplified with a forward primer specific for TRIM5 exon 5 and a reverse primer specific for CypA . Lane 1, kb+ DNA ladder; lane 2, dH 2 O; lane 3, M. fascicularis ; and lane 4, M. nemestrina . ( D ) Sequence of the M. fascicularis TRIM5 3′ UTR and retrotransposed CypA . Lowercase letters indicate the TRIM5 3′ UTR. Italicized and underlined letters identify the conserved retrotransposition sequence. Capital letters identify the retrotransposed CypA sequence in TRIMCyp. Bold letters highlight the start and stop codons of the CypA coding sequence.

    Article Snippet: Primers MneT5Aex51.23 (5′-GGTGTGGATGGCATCATTAAAAG-3′) and CypA*RMCSNotI were used to amplify a fragment of the TRIM5 gene from PBMC genomic DNA with Platinum PCR Super Mix High Fidelity (error rate: 1.8 ± 0.4 × 10−6 ; Invitrogen).

    Techniques: Polymerase Chain Reaction, Amplification, Sequencing

    Editing of CEP290 Transcripts Occurs in HEK293T after Transfection with PTMs (A) Illustration of adeno-associated virus genome arrangement of a CEP290 5′ PTM with binding domain 07 (PTM_07) with either no poly-adenylation signal (NPA) or with a bovine growth hormone poly-adenylation signal (PA). ITR, inverted terminal repeat; CMV, cytomegalovirus promoter; PCDS, partial coding DNA sequence of CEP290 ; 5′ SS, 5′ splice site. (B) qPCR was performed on HEK293T transfected with a plasmid encoding GFP as a transfection control or the plasmids in (A). TaqMan probes were designed to the junctions indicated: CEP290 exons 26 and 27 (hEx26-hEx27), a region within the 5′ codon optimized partial coding DNA sequence (coPCDS), or the novel junction of 5′ coPCDS and endogenous Homo sapiens exon 27 to signify trans -splicing (coPCDS-hEx27). Significant variation within replicates for coPCDS-hEx27 was present because the Ct was crossed at 35 cycles with either PTM; however, no amplification was observed through 40 cycles in GFP-treated samples. Samples were standardized to β-2-microglobin and normalized to NPA. Error bars are relative quantity minimum and maximum 95% confidence intervals. (C) Agarose gel electrophoresis of one of the replicate reactions from (B). (D) Densitometry analysis of the bands in (C). (E) Sanger sequencing following TOPO-cloning of the PCR product comprising the coPCDS-hEx27 junction visualized in (C). Nucleotide differences between Homo sapiens and codon-optimized CEP290 are noted by asterisks. The junction between the 5′ coPCDS and endogenous exon 27 is marked by a vertical dashed line.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Spliceosome-Mediated Pre-mRNA trans-Splicing Can Repair CEP290 mRNA

    doi: 10.1016/j.omtn.2018.05.014

    Figure Lengend Snippet: Editing of CEP290 Transcripts Occurs in HEK293T after Transfection with PTMs (A) Illustration of adeno-associated virus genome arrangement of a CEP290 5′ PTM with binding domain 07 (PTM_07) with either no poly-adenylation signal (NPA) or with a bovine growth hormone poly-adenylation signal (PA). ITR, inverted terminal repeat; CMV, cytomegalovirus promoter; PCDS, partial coding DNA sequence of CEP290 ; 5′ SS, 5′ splice site. (B) qPCR was performed on HEK293T transfected with a plasmid encoding GFP as a transfection control or the plasmids in (A). TaqMan probes were designed to the junctions indicated: CEP290 exons 26 and 27 (hEx26-hEx27), a region within the 5′ codon optimized partial coding DNA sequence (coPCDS), or the novel junction of 5′ coPCDS and endogenous Homo sapiens exon 27 to signify trans -splicing (coPCDS-hEx27). Significant variation within replicates for coPCDS-hEx27 was present because the Ct was crossed at 35 cycles with either PTM; however, no amplification was observed through 40 cycles in GFP-treated samples. Samples were standardized to β-2-microglobin and normalized to NPA. Error bars are relative quantity minimum and maximum 95% confidence intervals. (C) Agarose gel electrophoresis of one of the replicate reactions from (B). (D) Densitometry analysis of the bands in (C). (E) Sanger sequencing following TOPO-cloning of the PCR product comprising the coPCDS-hEx27 junction visualized in (C). Nucleotide differences between Homo sapiens and codon-optimized CEP290 are noted by asterisks. The junction between the 5′ coPCDS and endogenous exon 27 is marked by a vertical dashed line.

    Article Snippet: Reverse transcription was performed with Superscript III 1st Strand Synthesis (Thermo Fisher Scientific). qPCR was performed with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA) with cycling performed in an Applied Biosystems 7500 Fast Real-Time PCR system.

    Techniques: Transfection, Binding Assay, Sequencing, Real-time Polymerase Chain Reaction, Plasmid Preparation, Amplification, Agarose Gel Electrophoresis, Clone Assay, Polymerase Chain Reaction

    Editing of Mini- CEP290 Transcripts Occurs In Vivo following Sub-retinal Injection of 7m8AAV-5′ PTMs (A) Diagram of the CEP290 intron 26 mini-gene. The mini-gene is driven by the murine Rhodopsin promoter (mRho). CEP290 exons 25 and 26 are joined and followed by the complete intron 26-27 and exon 27. An A > G mutation corresponding to c.2991+1655 was included to assess exon X splicing in the model. An amino-terminal Myc and a carboxy-terminal FLAG tag were added to flank the mini-gene. Additionally, an internal ribosomal entry site (IRES) was added to drive expression of EGFP and is terminated by a bovine growth hormone poly-adenylation signal sequence (pA). (B) Immunofluorescence staining of a retinal cross-section from a mini- CEP290 mouse. OS, outer segment. ONL, outer nuclear layer. INL, inner nuclear layer. Scale bar is 50 μm. (C) Illustration of potential splicing outcomes within the mini- CEP290 mouse. Canonical cis- splicing would result in a predicted 24.3-kDa peptide. Alternative cis- splicing with exon X would yield a predicted non-sense media decay transcript encoding a truncated peptide with Myc at 17.1 kDa and no FLAG translation. Trans -splicing with a 5′ PTM would replace the amino-terminal Myc and generate a 124.1-kDa peptide with a FLAG tag. Arrow pairs indicate locations of PCR primers used to detect specific splicing events. (D) Representative western blot images of Myc and FLAG at 24.3 kDa showing OD and OS lanes per animal for each contralateral treatment cohort. (E) Densitometry quantification showing the mean log 10 values of each contralateral treatment cohort. Samples were standardized to α-tubulin. Error bars are SEM. Sample sizes as indicated. Individual animal data is available in Figure S1 A. (F) qPCR from cDNA generated by RNA extracts from whole eyes of mini- CEP290 mice. TaqMan probes were designed to the junctions of Homo sapiens exon 27 and FLAG to detect total expression of the mini-gene (left) to a region within the PCDS to detect total expression of the PTM (center) or to the novel junction of codon-optimized CEP290 PCDS and Homo sapiens CEP290 exon 27 (right). Values from treatment-matched samples were averaged as biological groups, standardized to murine β-2-microglobin and normalized to PTM_NBD. p

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Spliceosome-Mediated Pre-mRNA trans-Splicing Can Repair CEP290 mRNA

    doi: 10.1016/j.omtn.2018.05.014

    Figure Lengend Snippet: Editing of Mini- CEP290 Transcripts Occurs In Vivo following Sub-retinal Injection of 7m8AAV-5′ PTMs (A) Diagram of the CEP290 intron 26 mini-gene. The mini-gene is driven by the murine Rhodopsin promoter (mRho). CEP290 exons 25 and 26 are joined and followed by the complete intron 26-27 and exon 27. An A > G mutation corresponding to c.2991+1655 was included to assess exon X splicing in the model. An amino-terminal Myc and a carboxy-terminal FLAG tag were added to flank the mini-gene. Additionally, an internal ribosomal entry site (IRES) was added to drive expression of EGFP and is terminated by a bovine growth hormone poly-adenylation signal sequence (pA). (B) Immunofluorescence staining of a retinal cross-section from a mini- CEP290 mouse. OS, outer segment. ONL, outer nuclear layer. INL, inner nuclear layer. Scale bar is 50 μm. (C) Illustration of potential splicing outcomes within the mini- CEP290 mouse. Canonical cis- splicing would result in a predicted 24.3-kDa peptide. Alternative cis- splicing with exon X would yield a predicted non-sense media decay transcript encoding a truncated peptide with Myc at 17.1 kDa and no FLAG translation. Trans -splicing with a 5′ PTM would replace the amino-terminal Myc and generate a 124.1-kDa peptide with a FLAG tag. Arrow pairs indicate locations of PCR primers used to detect specific splicing events. (D) Representative western blot images of Myc and FLAG at 24.3 kDa showing OD and OS lanes per animal for each contralateral treatment cohort. (E) Densitometry quantification showing the mean log 10 values of each contralateral treatment cohort. Samples were standardized to α-tubulin. Error bars are SEM. Sample sizes as indicated. Individual animal data is available in Figure S1 A. (F) qPCR from cDNA generated by RNA extracts from whole eyes of mini- CEP290 mice. TaqMan probes were designed to the junctions of Homo sapiens exon 27 and FLAG to detect total expression of the mini-gene (left) to a region within the PCDS to detect total expression of the PTM (center) or to the novel junction of codon-optimized CEP290 PCDS and Homo sapiens CEP290 exon 27 (right). Values from treatment-matched samples were averaged as biological groups, standardized to murine β-2-microglobin and normalized to PTM_NBD. p

    Article Snippet: Reverse transcription was performed with Superscript III 1st Strand Synthesis (Thermo Fisher Scientific). qPCR was performed with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA) with cycling performed in an Applied Biosystems 7500 Fast Real-Time PCR system.

    Techniques: In Vivo, Injection, Mutagenesis, FLAG-tag, Expressing, Sequencing, Immunofluorescence, Staining, Polymerase Chain Reaction, Western Blot, Real-time Polymerase Chain Reaction, Generated, Mouse Assay

    Effect of CIB1 knockdown on de novo KSHV infection. (A) Untransduced, control or CIB1-shRNA transduced HMVEC-d cells were infected with KSHV (20 DNA copies/cell). At 24 h p.i., cells were harvested, total RNA was isolated, and viral gene expression was determined by real-time RT-PCR with KSHV ORF73 gene specific primers. Results are presented as percentage of inhibition of KSHV gene expression by sh-CIB1 or control compared with the infected untransduced cells. ***P = 0.0001. (B) (i) Control or CIB1-shRNA transduced HMVEC-d cells were mock or KSHV infected (20 DNA copies/cell) for 2 h at 37°C, washed to remove unbound viruses, and continued to culture for another 46 h. At 48 h p.i., cells were processed for immunofluorescence analysis using mouse anti-LANA-1 antibodies and co-stained with DAPI. Representative images are shown. (B) (ii) The percentage of cells observed positive for characteristic punctate LANA-1 staining in IFA is presented graphically. A minimum of three independent fields, each with at least 10 cells were chosen. Error bars show ± SD. (C) Control or CIB1-shRNA transduced HMVEC-d cells were mock or HSV-1 infected (3 pfu/cell) for 2 h at 37°C, washed to remove unbound viruses, and incubated for another 6 h. At 8 h p.i., cells were harvested, total RNA was isolated, and HSV-1 gene expression was quantified by SYBR green q-PCR method with ICP0 and ICP4 gene specific primers. Results are presented as fold HSV-1 gene expression normalized to internal tubulin control. Error bars show ± SD. (D) 293 cells were either mock-transfected or transfected with CIB1 overexpressing vector pcDNA-CIB1-Myc using lipofectamine. At 48 h post-transfection, CIB1 overexpression was examined by Western blotting with rabbit anti-CIB1 and mouse anti-Myc antibodies. Actin was used as loading control. (E) At 48 h post-transfection, transfected 293 cells were infected with KSHV (20 DNA copies/cell) for 2 h at 37°C for entry experiments. Post-washing, total DNA was isolated and KSHV entry was determined by real-time DNA-PCR for the ORF73 gene. Each reaction was done in triplicate and each bar represents the average ± SD of three independent experiments. Results are presented as percentage increase in KSHV DNA internalization in pcDNA-CIB1-Myc expressing cells compared with the control vector transfected cells, which is considered as 100%.

    Journal: PLoS Pathogens

    Article Title: CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells

    doi: 10.1371/journal.ppat.1003941

    Figure Lengend Snippet: Effect of CIB1 knockdown on de novo KSHV infection. (A) Untransduced, control or CIB1-shRNA transduced HMVEC-d cells were infected with KSHV (20 DNA copies/cell). At 24 h p.i., cells were harvested, total RNA was isolated, and viral gene expression was determined by real-time RT-PCR with KSHV ORF73 gene specific primers. Results are presented as percentage of inhibition of KSHV gene expression by sh-CIB1 or control compared with the infected untransduced cells. ***P = 0.0001. (B) (i) Control or CIB1-shRNA transduced HMVEC-d cells were mock or KSHV infected (20 DNA copies/cell) for 2 h at 37°C, washed to remove unbound viruses, and continued to culture for another 46 h. At 48 h p.i., cells were processed for immunofluorescence analysis using mouse anti-LANA-1 antibodies and co-stained with DAPI. Representative images are shown. (B) (ii) The percentage of cells observed positive for characteristic punctate LANA-1 staining in IFA is presented graphically. A minimum of three independent fields, each with at least 10 cells were chosen. Error bars show ± SD. (C) Control or CIB1-shRNA transduced HMVEC-d cells were mock or HSV-1 infected (3 pfu/cell) for 2 h at 37°C, washed to remove unbound viruses, and incubated for another 6 h. At 8 h p.i., cells were harvested, total RNA was isolated, and HSV-1 gene expression was quantified by SYBR green q-PCR method with ICP0 and ICP4 gene specific primers. Results are presented as fold HSV-1 gene expression normalized to internal tubulin control. Error bars show ± SD. (D) 293 cells were either mock-transfected or transfected with CIB1 overexpressing vector pcDNA-CIB1-Myc using lipofectamine. At 48 h post-transfection, CIB1 overexpression was examined by Western blotting with rabbit anti-CIB1 and mouse anti-Myc antibodies. Actin was used as loading control. (E) At 48 h post-transfection, transfected 293 cells were infected with KSHV (20 DNA copies/cell) for 2 h at 37°C for entry experiments. Post-washing, total DNA was isolated and KSHV entry was determined by real-time DNA-PCR for the ORF73 gene. Each reaction was done in triplicate and each bar represents the average ± SD of three independent experiments. Results are presented as percentage increase in KSHV DNA internalization in pcDNA-CIB1-Myc expressing cells compared with the control vector transfected cells, which is considered as 100%.

    Article Snippet: Equal volumes of prepared cDNA were used for quantification of HSV-1 gene ICP0 and ICP4 transcripts with the Power SYBR Green PCR master mix (Applied biosystems) according to the manufacturer's protocol.

    Techniques: Infection, shRNA, Isolation, Expressing, Quantitative RT-PCR, Inhibition, Immunofluorescence, Staining, Incubation, SYBR Green Assay, Polymerase Chain Reaction, Transfection, Plasmid Preparation, Over Expression, Western Blot

    Correlation of array matrix data to quantitative real-time PCR. Labeled RNA samples were made from human and brain total RNA. These were hybridized to separate array matrices containing 633 human genes. Six technical replicates were included for each sample. Twenty-one genes from this list were selected for analysis by TaqMan quantitative real-time PCR. A scatter plot of hybridization intensities of the liver and brain samples on the array matrix is shown in A . Genes selected for further analysis are shaded orange. A scatter plot of log-transformed hybridization signal ratios as determined by the two methods is shown in B .

    Journal: Genome Research

    Article Title: A novel, high-performance random array platform for quantitative gene expression profiling

    doi: 10.1101/gr.2739104

    Figure Lengend Snippet: Correlation of array matrix data to quantitative real-time PCR. Labeled RNA samples were made from human and brain total RNA. These were hybridized to separate array matrices containing 633 human genes. Six technical replicates were included for each sample. Twenty-one genes from this list were selected for analysis by TaqMan quantitative real-time PCR. A scatter plot of hybridization intensities of the liver and brain samples on the array matrix is shown in A . Genes selected for further analysis are shaded orange. A scatter plot of log-transformed hybridization signal ratios as determined by the two methods is shown in B .

    Article Snippet: Assays-on-Demand quantitative gene expression primers and TaqMan universal PCR master mix (Cat. #4304437) were purchased from Applied Biosystems.

    Techniques: Real-time Polymerase Chain Reaction, Labeling, Hybridization, Transformation Assay

    An example of the SYBR Green RD-qPCR assay of 5-LOX DNA methylation (shown are graphs obtained from 10 cerebellar samples). The samples were digested with the methylation-sensitive endonucleases (AciI, BstUI, HinP1I, and HpaII) as described in Material and Methods. The PCR reaction for the promoter-5′UTR (10 samples each: AciI = blue, BstUI = red, HinP1I = green, and HpaII = gray) and corresponding input control regions (shown in yellow) were carried out in separate tubes. Panel A shows the dissociation curve data, which indicate the presence of only one PCR product (peak) for each specific set of primers (fluorescence (first derivative of the raw fluorescence reading multiplied by −1) on the Y-axis versus the PCR product melting temperature (°C) on the X-axis). Panel B shows examples of the amplification plots used for calculating the quantitative data (the amplification plots fluorescence (baseline-corrected raw fluorescence) on the Y-axis versus cycle number on the X-axis). In this assay, the threshold cycle is inversely proportional to the log of the initial copy number. In other words, the more template that is present initially, the fewer the number of cycles required for the fluorescence signal to be detectable above background.

    Journal: Neural Plasticity

    Article Title: 5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice

    doi: 10.1155/2009/209596

    Figure Lengend Snippet: An example of the SYBR Green RD-qPCR assay of 5-LOX DNA methylation (shown are graphs obtained from 10 cerebellar samples). The samples were digested with the methylation-sensitive endonucleases (AciI, BstUI, HinP1I, and HpaII) as described in Material and Methods. The PCR reaction for the promoter-5′UTR (10 samples each: AciI = blue, BstUI = red, HinP1I = green, and HpaII = gray) and corresponding input control regions (shown in yellow) were carried out in separate tubes. Panel A shows the dissociation curve data, which indicate the presence of only one PCR product (peak) for each specific set of primers (fluorescence (first derivative of the raw fluorescence reading multiplied by −1) on the Y-axis versus the PCR product melting temperature (°C) on the X-axis). Panel B shows examples of the amplification plots used for calculating the quantitative data (the amplification plots fluorescence (baseline-corrected raw fluorescence) on the Y-axis versus cycle number on the X-axis). In this assay, the threshold cycle is inversely proportional to the log of the initial copy number. In other words, the more template that is present initially, the fewer the number of cycles required for the fluorescence signal to be detectable above background.

    Article Snippet: Digested DNA samples were diluted with water and an aliquot (100 ng DNA) was used for qPCR (Stratagene) with the Maxima SYBR Green qPCR Master Mix (Fermentas) according to the manufacturer's protocol.

    Techniques: SYBR Green Assay, Real-time Polymerase Chain Reaction, DNA Methylation Assay, Methylation, Polymerase Chain Reaction, Fluorescence, Amplification