Journal: BMC Evolutionary Biology
Article Title: Comparative analyses of the Conserved Oligomeric Golgi (COG) complex in vertebrates
Figure Lengend Snippet: RT-PCR analyses of the eight COG genes expression in 20 human tissues . GAPDH was used as an internal control for the cDNA.
Article Snippet: PCR amplifications were performed using the QIAGEN multiplex PCR kit (Qiagen, Hilden, Germany) at 1× Qiagen multiplex PCR master mix with 0.5 μl of cDNA in a 12.5 μl final reaction volume.
Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing