pcr mix Promega Search Results


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  • 88
    Promega polymerase chain reaction pcr master mix
    Polymerase Chain Reaction Pcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega transcription polymerase chain reaction
    Transcription Polymerase Chain Reaction, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega gotaq polymerase chain reaction pcr mixture
    Gotaq Polymerase Chain Reaction Pcr Mixture, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega polymerase chain reaction master mix kit
    Polymerase Chain Reaction Master Mix Kit, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega polymerase chain reaction pcr master mix reagents
    Polymerase Chain Reaction Pcr Master Mix Reagents, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega sybr green polymerase chain reaction master mix
    Sybr Green Polymerase Chain Reaction Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega quantitative polymerase chain reaction qpcr master mix
    Quantitative Polymerase Chain Reaction Qpcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega gotaq quantitative real time polymerase chain reaction qpcr master mix
    Gotaq Quantitative Real Time Polymerase Chain Reaction Qpcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega gotaq quantitative polymerase chain reaction qpcr master mix
    Gotaq Quantitative Polymerase Chain Reaction Qpcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega promega pcr mix
    Methylation specific <t>PCR</t> to analyze the methylation status of Per1 and Per2 promoters. KATO III cells were treated with 3 mM NaB, 100 nM TSA for 48 h or 5 mM Aza for 96 h. Then <t>DNA</t> was subjected to bisulfite modification and PCR was conducted with specific primers to distinguish methylated (Lane M) and unmethylated (Lane U) forms of CpGs at the Per1 and Per2 promoters. PCR products were separated on 2% agarose gels. (A) MS-PCR of untreated control KATO III cells. (B) MS-PCR of NaB, TSA or Aza treated KATO III cells. (C) Reverse transcription-quantitative PCR showing upregulation of Per2 mRNA in Aza treated KATO III cells. ***P
    Promega Pcr Mix, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 644 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega promega gotaq mix
    Methylation specific <t>PCR</t> to analyze the methylation status of Per1 and Per2 promoters. KATO III cells were treated with 3 mM NaB, 100 nM TSA for 48 h or 5 mM Aza for 96 h. Then <t>DNA</t> was subjected to bisulfite modification and PCR was conducted with specific primers to distinguish methylated (Lane M) and unmethylated (Lane U) forms of CpGs at the Per1 and Per2 promoters. PCR products were separated on 2% agarose gels. (A) MS-PCR of untreated control KATO III cells. (B) MS-PCR of NaB, TSA or Aza treated KATO III cells. (C) Reverse transcription-quantitative PCR showing upregulation of Per2 mRNA in Aza treated KATO III cells. ***P
    Promega Gotaq Mix, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pcr mixture contained gotaq green master mix promega
    Methylation specific <t>PCR</t> to analyze the methylation status of Per1 and Per2 promoters. KATO III cells were treated with 3 mM NaB, 100 nM TSA for 48 h or 5 mM Aza for 96 h. Then <t>DNA</t> was subjected to bisulfite modification and PCR was conducted with specific primers to distinguish methylated (Lane M) and unmethylated (Lane U) forms of CpGs at the Per1 and Per2 promoters. PCR products were separated on 2% agarose gels. (A) MS-PCR of untreated control KATO III cells. (B) MS-PCR of NaB, TSA or Aza treated KATO III cells. (C) Reverse transcription-quantitative PCR showing upregulation of Per2 mRNA in Aza treated KATO III cells. ***P
    Pcr Mixture Contained Gotaq Green Master Mix Promega, supplied by Promega, used in various techniques. Bioz Stars score: 75/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega promega master mix
    Methylation specific <t>PCR</t> to analyze the methylation status of Per1 and Per2 promoters. KATO III cells were treated with 3 mM NaB, 100 nM TSA for 48 h or 5 mM Aza for 96 h. Then <t>DNA</t> was subjected to bisulfite modification and PCR was conducted with specific primers to distinguish methylated (Lane M) and unmethylated (Lane U) forms of CpGs at the Per1 and Per2 promoters. PCR products were separated on 2% agarose gels. (A) MS-PCR of untreated control KATO III cells. (B) MS-PCR of NaB, TSA or Aza treated KATO III cells. (C) Reverse transcription-quantitative PCR showing upregulation of Per2 mRNA in Aza treated KATO III cells. ***P
    Promega Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega promega hotmaster mix
    Methylation specific <t>PCR</t> to analyze the methylation status of Per1 and Per2 promoters. KATO III cells were treated with 3 mM NaB, 100 nM TSA for 48 h or 5 mM Aza for 96 h. Then <t>DNA</t> was subjected to bisulfite modification and PCR was conducted with specific primers to distinguish methylated (Lane M) and unmethylated (Lane U) forms of CpGs at the Per1 and Per2 promoters. PCR products were separated on 2% agarose gels. (A) MS-PCR of untreated control KATO III cells. (B) MS-PCR of NaB, TSA or Aza treated KATO III cells. (C) Reverse transcription-quantitative PCR showing upregulation of Per2 mRNA in Aza treated KATO III cells. ***P
    Promega Hotmaster Mix, supplied by Promega, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pcr nucleotide mix
    Analysis of <t>HIV-1</t> population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using <t>LP-PCR</t> or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B
    Pcr Nucleotide Mix, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pcr reaction mix
    Analysis of <t>HIV-1</t> population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using <t>LP-PCR</t> or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B
    Pcr Reaction Mix, supplied by Promega, used in various techniques. Bioz Stars score: 98/100, based on 570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega gotaq promega qpcr mix
    Analysis of <t>HIV-1</t> population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using <t>LP-PCR</t> or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B
    Gotaq Promega Qpcr Mix, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega taq polymerase gotaq green master mix promega
    Analysis of <t>HIV-1</t> population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using <t>LP-PCR</t> or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B
    Taq Polymerase Gotaq Green Master Mix Promega, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega promega pcr mastermix
    Analysis of <t>HIV-1</t> population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using <t>LP-PCR</t> or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B
    Promega Pcr Mastermix, supplied by Promega, used in various techniques. Bioz Stars score: 83/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega promega pcr buffer
    Analysis of <t>HIV-1</t> population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using <t>LP-PCR</t> or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B
    Promega Pcr Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pcr green master mix
    Analysis of <t>HIV-1</t> population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using <t>LP-PCR</t> or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B
    Pcr Green Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega gotaq green pcr mix
    Analysis of <t>HIV-1</t> population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using <t>LP-PCR</t> or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B
    Gotaq Green Pcr Mix, supplied by Promega, used in various techniques. Bioz Stars score: 84/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega gotaq pcr reaction mix
    Analysis of <t>HIV-1</t> population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using <t>LP-PCR</t> or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B
    Gotaq Pcr Reaction Mix, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega master mix 1x pcr
    Analysis of <t>HIV-1</t> population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using <t>LP-PCR</t> or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B
    Master Mix 1x Pcr, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega sybr green pcr mix
    Analysis of <t>HIV-1</t> population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using <t>LP-PCR</t> or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B
    Sybr Green Pcr Mix, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pcr mix solution
    Positive nested <t>PCR</t> result from hepatitis B surface-negative, core-positive serum samples of healthy volunteers. A 656 bp <t>DNA</t> product was amplified using primers specific for hepatitis B S-gene. All lanes show a PCR product of the expected size. LD: 100 bp molecular weight marker; PC: Positive control; and NTC: Non-template control (NTC). 160×161mm (72 × 72 DPI)
    Pcr Mix Solution, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega fresh pcr reaction mix
    The generation of DNA templates from microarrays and parallel analysis. A ssDNA microarray was incubated with a primer (16 h) followed by elongation using <t>Taq</t> polymerase (16 h) producing as dsDNA microarray. The newly synthesized DNA strands were used as templates for solution phase <t>PCR</t> carried out over the microarray leading to amplification of the ssDNA displayed on the microarray. The dsDNA was amplified by PCR to produce fluorescently labeled ssDNA analogous to the ssDNA printed on the microarray. The fluorescently labeled ssDNA was hybridized to a complementary microarray or submitted to Solexa sequencing to allow decoding of the amplified ssDNA. FAM = 5(6)-carboxyfluorescein.
    Fresh Pcr Reaction Mix, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega gmm go taq green master mix catalogue no m7113 promega
    The generation of DNA templates from microarrays and parallel analysis. A ssDNA microarray was incubated with a primer (16 h) followed by elongation using <t>Taq</t> polymerase (16 h) producing as dsDNA microarray. The newly synthesized DNA strands were used as templates for solution phase <t>PCR</t> carried out over the microarray leading to amplification of the ssDNA displayed on the microarray. The dsDNA was amplified by PCR to produce fluorescently labeled ssDNA analogous to the ssDNA printed on the microarray. The fluorescently labeled ssDNA was hybridized to a complementary microarray or submitted to Solexa sequencing to allow decoding of the amplified ssDNA. FAM = 5(6)-carboxyfluorescein.
    Gmm Go Taq Green Master Mix Catalogue No M7113 Promega, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega gotaq green master mix pcr reaction mixture
    The generation of DNA templates from microarrays and parallel analysis. A ssDNA microarray was incubated with a primer (16 h) followed by elongation using <t>Taq</t> polymerase (16 h) producing as dsDNA microarray. The newly synthesized DNA strands were used as templates for solution phase <t>PCR</t> carried out over the microarray leading to amplification of the ssDNA displayed on the microarray. The dsDNA was amplified by PCR to produce fluorescently labeled ssDNA analogous to the ssDNA printed on the microarray. The fluorescently labeled ssDNA was hybridized to a complementary microarray or submitted to Solexa sequencing to allow decoding of the amplified ssDNA. FAM = 5(6)-carboxyfluorescein.
    Gotaq Green Master Mix Pcr Reaction Mixture, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pcr green master mix buffer
    The generation of DNA templates from microarrays and parallel analysis. A ssDNA microarray was incubated with a primer (16 h) followed by elongation using <t>Taq</t> polymerase (16 h) producing as dsDNA microarray. The newly synthesized DNA strands were used as templates for solution phase <t>PCR</t> carried out over the microarray leading to amplification of the ssDNA displayed on the microarray. The dsDNA was amplified by PCR to produce fluorescently labeled ssDNA analogous to the ssDNA printed on the microarray. The fluorescently labeled ssDNA was hybridized to a complementary microarray or submitted to Solexa sequencing to allow decoding of the amplified ssDNA. FAM = 5(6)-carboxyfluorescein.
    Pcr Green Master Mix Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega pcr taq polymerase mix
    The generation of DNA templates from microarrays and parallel analysis. A ssDNA microarray was incubated with a primer (16 h) followed by elongation using <t>Taq</t> polymerase (16 h) producing as dsDNA microarray. The newly synthesized DNA strands were used as templates for solution phase <t>PCR</t> carried out over the microarray leading to amplification of the ssDNA displayed on the microarray. The dsDNA was amplified by PCR to produce fluorescently labeled ssDNA analogous to the ssDNA printed on the microarray. The fluorescently labeled ssDNA was hybridized to a complementary microarray or submitted to Solexa sequencing to allow decoding of the amplified ssDNA. FAM = 5(6)-carboxyfluorescein.
    Pcr Taq Polymerase Mix, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr taq polymerase mix/product/Promega
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    79
    Promega master mix pcr reagents
    Prominent tipifarnib-induced inhibition of MAPK signaling. (A,C) after Jurkat cells were treated for 72 hours with the indicated tipifarnib concentration in the presence of 5μM Q-VD-OPh, whole cell lysates were subjected to immunoblotting with antibodies that recognize the indicate polypeptides. Ribosomal protein S6 and Hsp90 served as loading controls. (B) After Jurkat cells were treated for 72 hours with the indicated tipifarnib concentration in the presence of 5μM Q-VD-OPh, <t>cDNA</t> was prepared and <t>RT-PCR</t> was performed using primers specific for Bim.
    Master Mix Pcr Reagents, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/master mix pcr reagents/product/Promega
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    Image Search Results


    Methylation specific PCR to analyze the methylation status of Per1 and Per2 promoters. KATO III cells were treated with 3 mM NaB, 100 nM TSA for 48 h or 5 mM Aza for 96 h. Then DNA was subjected to bisulfite modification and PCR was conducted with specific primers to distinguish methylated (Lane M) and unmethylated (Lane U) forms of CpGs at the Per1 and Per2 promoters. PCR products were separated on 2% agarose gels. (A) MS-PCR of untreated control KATO III cells. (B) MS-PCR of NaB, TSA or Aza treated KATO III cells. (C) Reverse transcription-quantitative PCR showing upregulation of Per2 mRNA in Aza treated KATO III cells. ***P

    Journal: Oncology Letters

    Article Title: Histone deacetylase inhibitors induce the expression of tumor suppressor genes Per1 and Per2 in human gastric cancer cells

    doi: 10.3892/ol.2018.8851

    Figure Lengend Snippet: Methylation specific PCR to analyze the methylation status of Per1 and Per2 promoters. KATO III cells were treated with 3 mM NaB, 100 nM TSA for 48 h or 5 mM Aza for 96 h. Then DNA was subjected to bisulfite modification and PCR was conducted with specific primers to distinguish methylated (Lane M) and unmethylated (Lane U) forms of CpGs at the Per1 and Per2 promoters. PCR products were separated on 2% agarose gels. (A) MS-PCR of untreated control KATO III cells. (B) MS-PCR of NaB, TSA or Aza treated KATO III cells. (C) Reverse transcription-quantitative PCR showing upregulation of Per2 mRNA in Aza treated KATO III cells. ***P

    Article Snippet: PCR reactions were conducted with 1 µl of modified DNA, 10 µl of 2× PCR mix (Promega Corporation), 5 pmoles of sense and antisense primers in a final volume of 20 µl.

    Techniques: Methylation, Polymerase Chain Reaction, Modification, Mass Spectrometry, Real-time Polymerase Chain Reaction

    Analysis of HIV-1 population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using LP-PCR or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B

    Journal: Retrovirology

    Article Title: Ultrasensitive single-genome sequencing: accurate, targeted, next generation sequencing of HIV-1 RNA

    doi: 10.1186/s12977-016-0321-6

    Figure Lengend Snippet: Analysis of HIV-1 population structure in a clinical sample using uSGS for library construction. Neighbor joining trees of HIV-1 pol sequence from donor 1 sample 1 and blow up of NJ subtree, showing clustering and linkage of WT at RT position 106 (V106V blue squares ) with 101Q ( blue square with black outline ). Numbers of identical sequences are shown in parentheses as this NJ tree was extracted from 1585 unique SGS, where identical sequences were collapsed. Within the highlighted subtree, note especially two sequences (2) in which WT RT codon 106, and rare mutations 101Q and 108I ( blue square with black and orange out line ) were found to be linked. Detection of such a rare linkage event would be virtually impossible using LP-PCR or conventional SGS. Data were obtained by NGS uSGS and rooted on Consensus B

    Article Snippet: RNAs were reverse transcribed in 50 μL reactions that included 30 nM final concentration of HIV-1 gene specific primer with Primer ID (GSPID 2834R), 500 μM dNTPs (Promega C1145), 1× First Strand Buffer, 1 mm DTT, 20 U RNase out (Promega Cat #N2115; 40 U/µl) and 200 U SuperScript III (Life Technologies, Cat #18080-044; 200 U/µl).

    Techniques: Sequencing, Polymerase Chain Reaction, Next-Generation Sequencing

    Positive nested PCR result from hepatitis B surface-negative, core-positive serum samples of healthy volunteers. A 656 bp DNA product was amplified using primers specific for hepatitis B S-gene. All lanes show a PCR product of the expected size. LD: 100 bp molecular weight marker; PC: Positive control; and NTC: Non-template control (NTC). 160×161mm (72 × 72 DPI)

    Journal: African Health Sciences

    Article Title: Molecular and serological detection of occult hepatitis B virus among healthy hepatitis B surface antigen-negative blood donors in Malaysia

    doi: 10.4314/ahs.v16i3.6

    Figure Lengend Snippet: Positive nested PCR result from hepatitis B surface-negative, core-positive serum samples of healthy volunteers. A 656 bp DNA product was amplified using primers specific for hepatitis B S-gene. All lanes show a PCR product of the expected size. LD: 100 bp molecular weight marker; PC: Positive control; and NTC: Non-template control (NTC). 160×161mm (72 × 72 DPI)

    Article Snippet: The second round of PCR was performed using the inner primers, which amplify the 656 bp S-gene amplicon of the surface antigen under the following conditions: 30 cycles of 94°C for 5 min, 94°C for 30 sec, 63.8°C for 30 sec, and 72°C for 60 sec, with a final extension at 72°C for 8 min. A 50 µl reaction mixture containing 1 µl of DNA sample, 25 µl PCR pre mixed solution (Promega, USA), 1.25 µl of forward and reverse primers(final concentration 0.5 µM), and 21.5 µl of nuclease free water.

    Techniques: Nested PCR, Amplification, Polymerase Chain Reaction, Molecular Weight, Marker, Positive Control

    The generation of DNA templates from microarrays and parallel analysis. A ssDNA microarray was incubated with a primer (16 h) followed by elongation using Taq polymerase (16 h) producing as dsDNA microarray. The newly synthesized DNA strands were used as templates for solution phase PCR carried out over the microarray leading to amplification of the ssDNA displayed on the microarray. The dsDNA was amplified by PCR to produce fluorescently labeled ssDNA analogous to the ssDNA printed on the microarray. The fluorescently labeled ssDNA was hybridized to a complementary microarray or submitted to Solexa sequencing to allow decoding of the amplified ssDNA. FAM = 5(6)-carboxyfluorescein.

    Journal: PLoS ONE

    Article Title: Microarray Generation of Thousand-Member Oligonucleotide Libraries

    doi: 10.1371/journal.pone.0024906

    Figure Lengend Snippet: The generation of DNA templates from microarrays and parallel analysis. A ssDNA microarray was incubated with a primer (16 h) followed by elongation using Taq polymerase (16 h) producing as dsDNA microarray. The newly synthesized DNA strands were used as templates for solution phase PCR carried out over the microarray leading to amplification of the ssDNA displayed on the microarray. The dsDNA was amplified by PCR to produce fluorescently labeled ssDNA analogous to the ssDNA printed on the microarray. The fluorescently labeled ssDNA was hybridized to a complementary microarray or submitted to Solexa sequencing to allow decoding of the amplified ssDNA. FAM = 5(6)-carboxyfluorescein.

    Article Snippet: Hereafter, the reaction mixture was removed using a pipette and fresh PCR reaction mix (200 µL; Promega, 25 U/mL Taq Polymerase, 200 µM dNTP, 1.5 mM MgCl2 ) with primer-1 and 2 (0.1 µM) or Solexa-primer-1 and 2 (0.1 µM) were loaded onto the microarray and a standard PCR cycle was set up in a GeneMachines® Hyb4 automated hybridizer [40 cycles, denaturation at 94°C for 30 s for 10 cycles and 88°C for 30 s for 30 cycles, annealing at 49°C for 1 min, elongation at 50°C for 5 min (primer-1 and 2), or denaturation at 94°C for 30 s, annealing at 65°C for 1 min, elongation at 70°C for 1 min (Solexa-primer-1 and 2)].

    Techniques: Microarray, Incubation, Synthesized, Polymerase Chain Reaction, Amplification, Labeling, Sequencing

    Prominent tipifarnib-induced inhibition of MAPK signaling. (A,C) after Jurkat cells were treated for 72 hours with the indicated tipifarnib concentration in the presence of 5μM Q-VD-OPh, whole cell lysates were subjected to immunoblotting with antibodies that recognize the indicate polypeptides. Ribosomal protein S6 and Hsp90 served as loading controls. (B) After Jurkat cells were treated for 72 hours with the indicated tipifarnib concentration in the presence of 5μM Q-VD-OPh, cDNA was prepared and RT-PCR was performed using primers specific for Bim.

    Journal: Blood

    Article Title: Cytotoxicity of farnesyltransferase inhibitors in lymphoid cells mediated by MAPK pathway inhibition and Bim up-regulation

    doi: 10.1182/blood-2011-02-334870

    Figure Lengend Snippet: Prominent tipifarnib-induced inhibition of MAPK signaling. (A,C) after Jurkat cells were treated for 72 hours with the indicated tipifarnib concentration in the presence of 5μM Q-VD-OPh, whole cell lysates were subjected to immunoblotting with antibodies that recognize the indicate polypeptides. Ribosomal protein S6 and Hsp90 served as loading controls. (B) After Jurkat cells were treated for 72 hours with the indicated tipifarnib concentration in the presence of 5μM Q-VD-OPh, cDNA was prepared and RT-PCR was performed using primers specific for Bim.

    Article Snippet: PCR reactions (50 μL) were performed using 3 μg of cDNA product and Master Mix PCR reagents (Promega) according to the supplier's instructions.

    Techniques: Inhibition, Concentration Assay, Reverse Transcription Polymerase Chain Reaction