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  • 94
    Meridian Life Science pcr mixture
    Induction of pdc mRNA in Lactobacillus strains, as demonstrated by <t>RT-PCR.</t> (A) RT-PCR products from L. pentosus 128 mRNA after exposure of cells to FA at 100 μg/ml for 5 min (lane 1) and 30 min (lane 2) and to PCA at 100 μg/ml for 0 min (lane 4), 5 min (lane 5), and 30 min (lane 6). Ten microliters of RT-PCR product was loaded on the gel. Lane 3, PCR <t>(DNA</t> template) control; lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. crispatus H8 mRNA after exposure of cells to PCA at 100 μg/ml for 0 min (lane 2), 5 min (lane 3), and 30 min (lane 4). Twenty microliters of RT-PCR product was loaded on the gel. Lane 1, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco).
    Pcr Mixture, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 94/100, based on 801 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Meridian Life Science mytaq red mix
    Induction of pdc mRNA in Lactobacillus strains, as demonstrated by <t>RT-PCR.</t> (A) RT-PCR products from L. pentosus 128 mRNA after exposure of cells to FA at 100 μg/ml for 5 min (lane 1) and 30 min (lane 2) and to PCA at 100 μg/ml for 0 min (lane 4), 5 min (lane 5), and 30 min (lane 6). Ten microliters of RT-PCR product was loaded on the gel. Lane 3, PCR <t>(DNA</t> template) control; lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. crispatus H8 mRNA after exposure of cells to PCA at 100 μg/ml for 0 min (lane 2), 5 min (lane 3), and 30 min (lane 4). Twenty microliters of RT-PCR product was loaded on the gel. Lane 1, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco).
    Mytaq Red Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 91/100, based on 1026 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Meridian Life Science sensifast sybr no rox mix
    Induction of pdc mRNA in Lactobacillus strains, as demonstrated by <t>RT-PCR.</t> (A) RT-PCR products from L. pentosus 128 mRNA after exposure of cells to FA at 100 μg/ml for 5 min (lane 1) and 30 min (lane 2) and to PCA at 100 μg/ml for 0 min (lane 4), 5 min (lane 5), and 30 min (lane 6). Ten microliters of RT-PCR product was loaded on the gel. Lane 3, PCR <t>(DNA</t> template) control; lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. crispatus H8 mRNA after exposure of cells to PCA at 100 μg/ml for 0 min (lane 2), 5 min (lane 3), and 30 min (lane 4). Twenty microliters of RT-PCR product was loaded on the gel. Lane 1, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco).
    Sensifast Sybr No Rox Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 89/100, based on 387 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Meridian Life Science dntp mix
    Induction of pdc mRNA in Lactobacillus strains, as demonstrated by <t>RT-PCR.</t> (A) RT-PCR products from L. pentosus 128 mRNA after exposure of cells to FA at 100 μg/ml for 5 min (lane 1) and 30 min (lane 2) and to PCA at 100 μg/ml for 0 min (lane 4), 5 min (lane 5), and 30 min (lane 6). Ten microliters of RT-PCR product was loaded on the gel. Lane 3, PCR <t>(DNA</t> template) control; lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. crispatus H8 mRNA after exposure of cells to PCA at 100 μg/ml for 0 min (lane 2), 5 min (lane 3), and 30 min (lane 4). Twenty microliters of RT-PCR product was loaded on the gel. Lane 1, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco).
    Dntp Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 94/100, based on 970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Meridian Life Science pcr reaction mixture
    Agarose gel electrophoresis of <t>PCR</t> products amplified from whole insect <t>DNA</t> using primers complementary to regions encoding COIII and cytB . A, B, C, D, refers to different gene arrangement types; Y, ancestral arrangement. Lanes 1 and 8 molecular size markers; lane 2, Bemisia tabaci ; lane 3, Tetraleurodes acaciae ; lane 4, Neomaskellia andropogonis ; lane 5, Aleurochiton aceris ; lane 6, Trialeurodes vaporariorum ; and lane 7, Aleurodicus dugesii .
    Pcr Reaction Mixture, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 92/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Meridian Life Science sybr green pcr master mix
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Sybr Green Pcr Master Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 92/100, based on 213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Meridian Life Science sybr green master mix
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Sybr Green Master Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 92/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Meridian Life Science mytaqtm red mix
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Mytaqtm Red Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 89/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Meridian Life Science primer mix
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Primer Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 92/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Meridian Life Science pyrotaq evagreen qpcr mix plus
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Pyrotaq Evagreen Qpcr Mix Plus, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 88/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Meridian Life Science myfi mix
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Myfi Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 90/100, based on 236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Meridian Life Science bio x act short mix
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Bio X Act Short Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 88/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Meridian Life Science pcr buffer
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Pcr Buffer, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 94/100, based on 2148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Meridian Life Science cdna mix
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Cdna Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Meridian Life Science taq polymerase
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Taq Polymerase, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 94/100, based on 2409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Meridian Life Science mytaq red mix kit
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Mytaq Red Mix Kit, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 88/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Meridian Life Science sensifast probe hi rox mix
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Sensifast Probe Hi Rox Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 88/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Meridian Life Science sensimix sybr mix
    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after <t>PCR</t> products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with <t>Sybr</t> Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).
    Sensimix Sybr Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 88/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Induction of pdc mRNA in Lactobacillus strains, as demonstrated by RT-PCR. (A) RT-PCR products from L. pentosus 128 mRNA after exposure of cells to FA at 100 μg/ml for 5 min (lane 1) and 30 min (lane 2) and to PCA at 100 μg/ml for 0 min (lane 4), 5 min (lane 5), and 30 min (lane 6). Ten microliters of RT-PCR product was loaded on the gel. Lane 3, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. crispatus H8 mRNA after exposure of cells to PCA at 100 μg/ml for 0 min (lane 2), 5 min (lane 3), and 30 min (lane 4). Twenty microliters of RT-PCR product was loaded on the gel. Lane 1, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco).

    Journal: Applied and Environmental Microbiology

    Article Title: Decarboxylation of Substituted Cinnamic Acids by Lactic Acid Bacteria Isolated during Malt Whisky Fermentation

    doi:

    Figure Lengend Snippet: Induction of pdc mRNA in Lactobacillus strains, as demonstrated by RT-PCR. (A) RT-PCR products from L. pentosus 128 mRNA after exposure of cells to FA at 100 μg/ml for 5 min (lane 1) and 30 min (lane 2) and to PCA at 100 μg/ml for 0 min (lane 4), 5 min (lane 5), and 30 min (lane 6). Ten microliters of RT-PCR product was loaded on the gel. Lane 3, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. crispatus H8 mRNA after exposure of cells to PCA at 100 μg/ml for 0 min (lane 2), 5 min (lane 3), and 30 min (lane 4). Twenty microliters of RT-PCR product was loaded on the gel. Lane 1, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco).

    Article Snippet: The PCR mixture contained 5 μl of 10× Taq DNA polymerase buffer, 1 μl of deoxynucleoside triphosphate mix (12.5 mM), 2 mM MgCl2 , 100 nM each primer, 20 ng of genomic template DNA, and 1 U of Taq DNA polymerase (Bioline, London, United Kingdom) in a final volume of 50 μl.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Marker

    Induction of pdc mRNA in Lactobacillus strains grown in distiller's wort, as demonstrated by RT-PCR. (A) RT-PCR products from L. fermentum 70 mRNA (lanes 1 to 3) and L. pentosus 128 mRNA (lanes 4 to 6) after exposure for 1 h to wort alone (lanes 1 and 4), wort supplemented with FA at 100 μg/ml (lanes 2 and 5), or wort supplemented with PCA at 100 μg/ml (lanes 3 and 6). Lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. casei 69 mRNA after exposure for 1 h to wort alone (lanes 2 and 4) or wort supplemented with FA at 100 μg/ml (lanes 1 and 3). Lane M, 100-bp DNA molecular size marker (Gibco).

    Journal: Applied and Environmental Microbiology

    Article Title: Decarboxylation of Substituted Cinnamic Acids by Lactic Acid Bacteria Isolated during Malt Whisky Fermentation

    doi:

    Figure Lengend Snippet: Induction of pdc mRNA in Lactobacillus strains grown in distiller's wort, as demonstrated by RT-PCR. (A) RT-PCR products from L. fermentum 70 mRNA (lanes 1 to 3) and L. pentosus 128 mRNA (lanes 4 to 6) after exposure for 1 h to wort alone (lanes 1 and 4), wort supplemented with FA at 100 μg/ml (lanes 2 and 5), or wort supplemented with PCA at 100 μg/ml (lanes 3 and 6). Lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. casei 69 mRNA after exposure for 1 h to wort alone (lanes 2 and 4) or wort supplemented with FA at 100 μg/ml (lanes 1 and 3). Lane M, 100-bp DNA molecular size marker (Gibco).

    Article Snippet: The PCR mixture contained 5 μl of 10× Taq DNA polymerase buffer, 1 μl of deoxynucleoside triphosphate mix (12.5 mM), 2 mM MgCl2 , 100 nM each primer, 20 ng of genomic template DNA, and 1 U of Taq DNA polymerase (Bioline, London, United Kingdom) in a final volume of 50 μl.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Marker

    Electrophoresis of polymerase chain reaction products using primers H1/H2, H3/H4, and FibF/FibR with fragment sizes of (A) 1219 bp, (B) 1319 bp, and (C) 1124 bp amplifying hexon and fiber gene regions of fowl adenovirus (FAdV) in 1% agarose gel from a sample of the original (passage 2 [E2]) and passaged FAdV isolates in specific pathogen-free chicken embryonated eggs (SPF CEE). Lane M, DNA marker 1 kb; Lane 1, positive control (UPM04217); Lane 2, E2 FAdV; Lane 3, E5 FAdV; Lane 4, E10 FAdV; Lane 5, E15 FAdV; Lane 6, E20 FAdV; Lane 7, negative control.

    Journal: Journal of Veterinary Science

    Article Title: Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens

    doi: 10.4142/jvs.2018.19.6.759

    Figure Lengend Snippet: Electrophoresis of polymerase chain reaction products using primers H1/H2, H3/H4, and FibF/FibR with fragment sizes of (A) 1219 bp, (B) 1319 bp, and (C) 1124 bp amplifying hexon and fiber gene regions of fowl adenovirus (FAdV) in 1% agarose gel from a sample of the original (passage 2 [E2]) and passaged FAdV isolates in specific pathogen-free chicken embryonated eggs (SPF CEE). Lane M, DNA marker 1 kb; Lane 1, positive control (UPM04217); Lane 2, E2 FAdV; Lane 3, E5 FAdV; Lane 4, E10 FAdV; Lane 5, E15 FAdV; Lane 6, E20 FAdV; Lane 7, negative control.

    Article Snippet: Amplification of DNA for detection of FAdV was conducted by using a PCR master mix (Bioline, UK) according to the manufacturer's protocol.

    Techniques: Electrophoresis, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker, Positive Control, Negative Control

    Agarose gel electrophoresis of PCR products amplified from whole insect DNA using primers complementary to regions encoding COIII and cytB . A, B, C, D, refers to different gene arrangement types; Y, ancestral arrangement. Lanes 1 and 8 molecular size markers; lane 2, Bemisia tabaci ; lane 3, Tetraleurodes acaciae ; lane 4, Neomaskellia andropogonis ; lane 5, Aleurochiton aceris ; lane 6, Trialeurodes vaporariorum ; and lane 7, Aleurodicus dugesii .

    Journal: BMC Evolutionary Biology

    Article Title: Organization of the mitochondrial genomes of whiteflies, aphids, and psyllids (Hemiptera, Sternorrhyncha)

    doi: 10.1186/1471-2148-4-25

    Figure Lengend Snippet: Agarose gel electrophoresis of PCR products amplified from whole insect DNA using primers complementary to regions encoding COIII and cytB . A, B, C, D, refers to different gene arrangement types; Y, ancestral arrangement. Lanes 1 and 8 molecular size markers; lane 2, Bemisia tabaci ; lane 3, Tetraleurodes acaciae ; lane 4, Neomaskellia andropogonis ; lane 5, Aleurochiton aceris ; lane 6, Trialeurodes vaporariorum ; and lane 7, Aleurodicus dugesii .

    Article Snippet: For fragments of 4 kb or less, the PCR reaction mixture (10 ul) contained 10 ng insect DNA, 1 ug bovine serum albumin, 5 mM MgCl2 , 0.2 mM dNTP, 10 pmoles of each primer, 0.6 U Bio-X-Act DNA polymerase, in Opti-Buffer (Bioline, London, United Kingdom).

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification

    Agarose gel electrophoresis of PCR products amplified from whole insect DNA using primers complementary to regions encoding COII and ND5 . A, B, C, D, refers to different gene arrangement types; Y, ancestral arrangement. Lanes 1 and 9 molecular size markers; lane 2, Bemisia tabaci ; lane 3, Tetraleurodes acaciae ; lane 4, Neomaskellia andropogonis ; lane 5, Aleurochiton aceris ; lane 6, Aleyrodes elevatus ; lane 7, Trialeurodes vaporariorum ; and lane 8, Aleurodicus dugesii .

    Journal: BMC Evolutionary Biology

    Article Title: Organization of the mitochondrial genomes of whiteflies, aphids, and psyllids (Hemiptera, Sternorrhyncha)

    doi: 10.1186/1471-2148-4-25

    Figure Lengend Snippet: Agarose gel electrophoresis of PCR products amplified from whole insect DNA using primers complementary to regions encoding COII and ND5 . A, B, C, D, refers to different gene arrangement types; Y, ancestral arrangement. Lanes 1 and 9 molecular size markers; lane 2, Bemisia tabaci ; lane 3, Tetraleurodes acaciae ; lane 4, Neomaskellia andropogonis ; lane 5, Aleurochiton aceris ; lane 6, Aleyrodes elevatus ; lane 7, Trialeurodes vaporariorum ; and lane 8, Aleurodicus dugesii .

    Article Snippet: For fragments of 4 kb or less, the PCR reaction mixture (10 ul) contained 10 ng insect DNA, 1 ug bovine serum albumin, 5 mM MgCl2 , 0.2 mM dNTP, 10 pmoles of each primer, 0.6 U Bio-X-Act DNA polymerase, in Opti-Buffer (Bioline, London, United Kingdom).

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification

    Summary of the major transpositions occurring in the mitochondria of whiteflies and the relationship of these changes to the phylogeny of whitefly species . The phylogenetic tree was obtained on the basis of combined mitochondrial cytB-N22-16S rDNA and Portiera endosymbiont 16S* and 23S* rDNA using the maximum likelihood method. Numbers at nodes correspond to bootstrap values after 500 replicates. The combination of the host and endosymbiont sequence data is justified by their cospeciation [6]. A, B, C, D indicate transposition type; Y, indicates mitochondria with an ancestral gene arrangement. Large arrowhead in mitochondrial genome indicates the original position of the transposed genes. Small arrowheads indicate the position of the insertion of the genes. Arrows outside circle indicate the direction of the transcription of the transposed genes. Arrow by the arrowhead of the B type transposition indicates the changed direction of transcription of the 12S rDNA . tRNAs have been omitted. (*), by species names indicate that the full mitochondrial genome was sequenced. (+), by species names indicates that a DNA fragment containing all or a part of the gene encoding for COIII and adjacent genes was sequenced. (o), by species name indicates that using oligonucleotide primers to COII and ND5 a PCR product was obtained corresponding to a size that was consistent with the presence of COIII-(tRNA-G)-ND3-(tRNAs-A-R-N) in the ancestral position (Fig. 8).

    Journal: BMC Evolutionary Biology

    Article Title: Organization of the mitochondrial genomes of whiteflies, aphids, and psyllids (Hemiptera, Sternorrhyncha)

    doi: 10.1186/1471-2148-4-25

    Figure Lengend Snippet: Summary of the major transpositions occurring in the mitochondria of whiteflies and the relationship of these changes to the phylogeny of whitefly species . The phylogenetic tree was obtained on the basis of combined mitochondrial cytB-N22-16S rDNA and Portiera endosymbiont 16S* and 23S* rDNA using the maximum likelihood method. Numbers at nodes correspond to bootstrap values after 500 replicates. The combination of the host and endosymbiont sequence data is justified by their cospeciation [6]. A, B, C, D indicate transposition type; Y, indicates mitochondria with an ancestral gene arrangement. Large arrowhead in mitochondrial genome indicates the original position of the transposed genes. Small arrowheads indicate the position of the insertion of the genes. Arrows outside circle indicate the direction of the transcription of the transposed genes. Arrow by the arrowhead of the B type transposition indicates the changed direction of transcription of the 12S rDNA . tRNAs have been omitted. (*), by species names indicate that the full mitochondrial genome was sequenced. (+), by species names indicates that a DNA fragment containing all or a part of the gene encoding for COIII and adjacent genes was sequenced. (o), by species name indicates that using oligonucleotide primers to COII and ND5 a PCR product was obtained corresponding to a size that was consistent with the presence of COIII-(tRNA-G)-ND3-(tRNAs-A-R-N) in the ancestral position (Fig. 8).

    Article Snippet: For fragments of 4 kb or less, the PCR reaction mixture (10 ul) contained 10 ng insect DNA, 1 ug bovine serum albumin, 5 mM MgCl2 , 0.2 mM dNTP, 10 pmoles of each primer, 0.6 U Bio-X-Act DNA polymerase, in Opti-Buffer (Bioline, London, United Kingdom).

    Techniques: Sequencing, Polymerase Chain Reaction

    Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after PCR products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with Sybr Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).

    Journal: PLoS ONE

    Article Title: Double strand RNA delivery system for plant-sap-feeding insects

    doi: 10.1371/journal.pone.0171861

    Figure Lengend Snippet: Analysis of dsRNA delivered through green beans. (A) dsRNA of LacZ gene (lane 2), JHAMT (lane 3), and Vg (lane 4), were obtained after PCR products from genomic DNA were amplified with primers containing T7 promoter sequence. These fragments were further transcribed using T7 RNA polymerase; the obtained in vitro transcribed dsRNA was confirmed by electrophoresis on 1% agarose and visualized by staining with Sybr Gold® (Life technologies) alongside a DNA ladder (Lane 1). (B) Total RNA extracted from green beans immersed in 5 μg of dsRNA for 1 day (Lanes 2–4) or 6 days (Lanes 5–7) was subjected to cDNA synthesis. 2 μg of this total RNA was subjected to RNase A digestion (0.5 μg/μl final) for 1 hr (Lanes 8–13). RT-PCR was performed on representative cDNAs of LacZ, JHAMT and Vg genes following electrophoresis on a 1% agarose gel and visualized by staining with Sybr Gold (Life technologies) (Lanes 2–13) alongside a DNA ladder (Lanes 1 and 15).

    Article Snippet: Quantitative real-time PCR analysis Levels of transcripts expressed were measured by quantitative realtime PCR (qPCR) using SYBR green PCR master mix from SensiMix SYBR® from Bioline (Taunton, MA).

    Techniques: Polymerase Chain Reaction, Amplification, Sequencing, In Vitro, Electrophoresis, Staining, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis