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  • 90
    Thermo Fisher amplitaq gold 360 pcr master mix
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    Roche polymerase chain reaction pcr mix
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    Thermo Fisher sybr pcr polymerase chain reaction master mix
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    Promega polymerase chain reaction pcr master mix
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    Arraystar polymerase chain reaction pcr master mix
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    Thermo Fisher polymerase chain reaction pcr master mix
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    TaKaRa polymerase chain reaction pcr master mix
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    Millipore polymerase chain reaction pcr mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Polymerase Chain Reaction Pcr Mix, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche sybr green polymerase chain reaction master mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
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    Kapa Biosystems polymerase chain reaction pcr mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
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    Thermo Fisher taqman universal polymerase chain reaction master mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Taqman Universal Polymerase Chain Reaction Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies quantitative polymerase chain reaction master mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Quantitative Polymerase Chain Reaction Master Mix, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 76/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene quantitative polymerase chain reaction master mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Quantitative Polymerase Chain Reaction Master Mix, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybrgreen polymerase chain reaction pcr master mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
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    tiangen biotech co sybr green polymerase chain reaction pcr real master mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Sybr Green Polymerase Chain Reaction Pcr Real Master Mix, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by PCR using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M DNA marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Immortalized mouse dental papilla mesenchymal cells preserve odontoblastic phenotype and respond to bone morphogenetic protein 2

    doi: 10.1007/s11626-013-9641-1

    Figure Lengend Snippet: Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by PCR using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M DNA marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.

    Article Snippet: Two-hundred nanograms of DNA (for pSV3 neo plasmid DNA 10 ng) were diluted in a 25-μl polymerase chain reaction (PCR) mix (Sigma–Aldrich).

    Techniques: Expressing, Amplification, Polymerase Chain Reaction, Staining, Marker, Negative Control, Immunohistochemistry, Western Blot, SDS Page

    Identification of SV40 transformation. ( A ) Genomic DNA in the primary and immortalized dental papilla mesenchymal cells was isolated and amplified by the SV40 specific primer. PCR products were run on an agarose gel and stained with ethidium bromide. Lane 1 , DNA marker (Low DNA mass ladder, Invitrogen); lane 2 , control; lane 3 , primary dental papilla mesenchymal cells; lane 4 , immortalized cells. ( B , C ) Immunohistochemical staining with antibody against simian virus 40 large T-antigen (SV40 T-Ag) in iMDP-3 cells and immunolabeling of SV40 protein was mostly present in the nucleus ( green color ; B ) whereas immunostaining was not seen in the primary cells ( C ). The cells were stained with DAPI for the nucleus. Con control, Pri primary, Imm immortalized.

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Immortalized mouse dental papilla mesenchymal cells preserve odontoblastic phenotype and respond to bone morphogenetic protein 2

    doi: 10.1007/s11626-013-9641-1

    Figure Lengend Snippet: Identification of SV40 transformation. ( A ) Genomic DNA in the primary and immortalized dental papilla mesenchymal cells was isolated and amplified by the SV40 specific primer. PCR products were run on an agarose gel and stained with ethidium bromide. Lane 1 , DNA marker (Low DNA mass ladder, Invitrogen); lane 2 , control; lane 3 , primary dental papilla mesenchymal cells; lane 4 , immortalized cells. ( B , C ) Immunohistochemical staining with antibody against simian virus 40 large T-antigen (SV40 T-Ag) in iMDP-3 cells and immunolabeling of SV40 protein was mostly present in the nucleus ( green color ; B ) whereas immunostaining was not seen in the primary cells ( C ). The cells were stained with DAPI for the nucleus. Con control, Pri primary, Imm immortalized.

    Article Snippet: Two-hundred nanograms of DNA (for pSV3 neo plasmid DNA 10 ng) were diluted in a 25-μl polymerase chain reaction (PCR) mix (Sigma–Aldrich).

    Techniques: Transformation Assay, Isolation, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Marker, Immunohistochemistry, Immunolabeling, Immunostaining