pcr mix Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs phusion high fidelity pcr master mix
    Phusion High Fidelity Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4975 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity pcr master mix/product/New England Biolabs
    Average 99 stars, based on 4975 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    96
    Thermo Fisher sybr green pcr master mix
    Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 101787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green pcr master mix/product/Thermo Fisher
    Average 96 stars, based on 101787 article reviews
    Price from $9.99 to $1999.99
    sybr green pcr master mix - by Bioz Stars, 2020-10
    96/100 stars
      Buy from Supplier

    99
    Thermo Fisher power sybr green pcr master mix
    Power Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 77430 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/power sybr green pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 77430 article reviews
    Price from $9.99 to $1999.99
    power sybr green pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher taqman universal pcr master mix
    Taqman Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 54482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 54482 article reviews
    Price from $9.99 to $1999.99
    taqman universal pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher pcr master mix
    Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8721 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 8721 article reviews
    Price from $9.99 to $1999.99
    pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher taqman fast universal pcr master mix
    Taqman Fast Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6842 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman fast universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 6842 article reviews
    Price from $9.99 to $1999.99
    taqman fast universal pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    92
    Toyobo sybr green real time pcr master mix
    Expression analysis of genes identified in the papilla by LM–RNA-seq. <t>qRT-PCR</t> was performed using the <t>SYBR</t> Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.
    Sybr Green Real Time Pcr Master Mix, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 6877 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green real time pcr master mix/product/Toyobo
    Average 92 stars, based on 6877 article reviews
    Price from $9.99 to $1999.99
    sybr green real time pcr master mix - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    99
    Thermo Fisher universal pcr master mix
    Expression analysis of genes identified in the papilla by LM–RNA-seq. <t>qRT-PCR</t> was performed using the <t>SYBR</t> Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.
    Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 6421 article reviews
    Price from $9.99 to $1999.99
    universal pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad sybr green pcr master mix
    Expression analysis of genes identified in the papilla by LM–RNA-seq. <t>qRT-PCR</t> was performed using the <t>SYBR</t> Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.
    Sybr Green Pcr Master Mix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 5927 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green pcr master mix/product/Bio-Rad
    Average 99 stars, based on 5927 article reviews
    Price from $9.99 to $1999.99
    sybr green pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Promega pcr master mix
    Expression analysis of genes identified in the papilla by LM–RNA-seq. <t>qRT-PCR</t> was performed using the <t>SYBR</t> Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.
    Pcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 5366 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr master mix/product/Promega
    Average 99 stars, based on 5366 article reviews
    Price from $9.99 to $1999.99
    pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Qiagen quantitect sybr green pcr master mix
    Expression analysis of genes identified in the papilla by LM–RNA-seq. <t>qRT-PCR</t> was performed using the <t>SYBR</t> Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.
    Quantitect Sybr Green Pcr Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5834 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect sybr green pcr master mix/product/Qiagen
    Average 99 stars, based on 5834 article reviews
    Price from $9.99 to $1999.99
    quantitect sybr green pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Qiagen multiplex pcr master mix
    Expression analysis of genes identified in the papilla by LM–RNA-seq. <t>qRT-PCR</t> was performed using the <t>SYBR</t> Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.
    Multiplex Pcr Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4530 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex pcr master mix/product/Qiagen
    Average 99 stars, based on 4530 article reviews
    Price from $9.99 to $1999.99
    multiplex pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    94
    Toyobo sybr green realtime pcr master mix
    Effect of inhalation of particles released from ultrasonic humidifier on mRNA expression of Ccl2 , and Cxcl1 in the lung. Expression levels of Ccl2 and Cxcl1 in the lung tissues (Experiments 3–5, Table 1 ) were analyzed by an Mx3000P (Agilent Technologies) with <t>SYBR</t> Green <t>Realtime</t> <t>PCR</t> Master Mix (Toyobo). Relative expression levels of the target genes were calculated for each sample after normalization against Gapdh . Statistical analysis was performed using an unpaired t -test and the level of significance was set at P
    Sybr Green Realtime Pcr Master Mix, supplied by Toyobo, used in various techniques. Bioz Stars score: 94/100, based on 3921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green realtime pcr master mix/product/Toyobo
    Average 94 stars, based on 3921 article reviews
    Price from $9.99 to $1999.99
    sybr green realtime pcr master mix - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    99
    Thermo Fisher taqman pcr master mix
    Effect of inhalation of particles released from ultrasonic humidifier on mRNA expression of Ccl2 , and Cxcl1 in the lung. Expression levels of Ccl2 and Cxcl1 in the lung tissues (Experiments 3–5, Table 1 ) were analyzed by an Mx3000P (Agilent Technologies) with <t>SYBR</t> Green <t>Realtime</t> <t>PCR</t> Master Mix (Toyobo). Relative expression levels of the target genes were calculated for each sample after normalization against Gapdh . Statistical analysis was performed using an unpaired t -test and the level of significance was set at P
    Taqman Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3011 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 3011 article reviews
    Price from $9.99 to $1999.99
    taqman pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher sybr green pcr master mix kit
    Effect of inhalation of particles released from ultrasonic humidifier on mRNA expression of Ccl2 , and Cxcl1 in the lung. Expression levels of Ccl2 and Cxcl1 in the lung tissues (Experiments 3–5, Table 1 ) were analyzed by an Mx3000P (Agilent Technologies) with <t>SYBR</t> Green <t>Realtime</t> <t>PCR</t> Master Mix (Toyobo). Relative expression levels of the target genes were calculated for each sample after normalization against Gapdh . Statistical analysis was performed using an unpaired t -test and the level of significance was set at P
    Sybr Green Pcr Master Mix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green pcr master mix kit/product/Thermo Fisher
    Average 99 stars, based on 4306 article reviews
    Price from $9.99 to $1999.99
    sybr green pcr master mix kit - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    94
    Toyobo sybr green pcr master mix
    Wnt5a , FZD 4, FZD5 and RHOU are significantly down-regulated in newly diagnosed AML patients’ bone marrow samples compared to normal or CR group. Expression levels of Wnt5a , FZD4 , FZD5 and RHOU were determined by <t>RT-PCR</t> analysis of bone marrow samples from AML patients, either newly-diagnosed (AML-ND) or in complete remission (AML-CR), and healthy donors (CON) using <t>SYBR</t> Green PCR Master Mix. The Mann–Whitney test was used to compare differences between the two groups.
    Sybr Green Pcr Master Mix, supplied by Toyobo, used in various techniques. Bioz Stars score: 94/100, based on 3055 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green pcr master mix/product/Toyobo
    Average 94 stars, based on 3055 article reviews
    Price from $9.99 to $1999.99
    sybr green pcr master mix - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    99
    New England Biolabs nebnext high fidelity 2x pcr master mix
    Wnt5a , FZD 4, FZD5 and RHOU are significantly down-regulated in newly diagnosed AML patients’ bone marrow samples compared to normal or CR group. Expression levels of Wnt5a , FZD4 , FZD5 and RHOU were determined by <t>RT-PCR</t> analysis of bone marrow samples from AML patients, either newly-diagnosed (AML-ND) or in complete remission (AML-CR), and healthy donors (CON) using <t>SYBR</t> Green PCR Master Mix. The Mann–Whitney test was used to compare differences between the two groups.
    Nebnext High Fidelity 2x Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext high fidelity 2x pcr master mix/product/New England Biolabs
    Average 99 stars, based on 1687 article reviews
    Price from $9.99 to $1999.99
    nebnext high fidelity 2x pcr master mix - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    94
    Bio-Rad pcr mix
    Linearity of the BCR-ABL <t>ddPCR</t> method when measuring ERM-AD623 samples within a <t>PCR</t> copy number concentration range of 2.5 cp/μL to 5400 cp/μL. The data points represent the average result for eight replicate measurements, and the vertical error bars represent the associated STD. The horizontal error bars represent the standard uncertainty associated with the certified values of the ERM-AD623 samples.
    Pcr Mix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr mix/product/Bio-Rad
    Average 94 stars, based on 837 article reviews
    Price from $9.99 to $1999.99
    pcr mix - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    Expression analysis of genes identified in the papilla by LM–RNA-seq. qRT-PCR was performed using the SYBR Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.

    Journal: Plant and Cell Physiology

    Article Title: Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing

    doi: 10.1093/pcp/pct133

    Figure Lengend Snippet: Expression analysis of genes identified in the papilla by LM–RNA-seq. qRT-PCR was performed using the SYBR Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.

    Article Snippet: Total RNA was reverse-transcribed using the SuperScript III First-Strand Synthesis system with oligo(dT) primer, according to the manufacturer’s instructions. qRT-PCR was performed using the SYBR Green Real-Time PCR Master Mix (Toyobo) with a Bio-Rad CFX96 Real-Time Detection System.

    Techniques: Expressing, RNA Sequencing Assay, Quantitative RT-PCR, SYBR Green Assay, Real-time Polymerase Chain Reaction

    Effect of inhalation of particles released from ultrasonic humidifier on mRNA expression of Ccl2 , and Cxcl1 in the lung. Expression levels of Ccl2 and Cxcl1 in the lung tissues (Experiments 3–5, Table 1 ) were analyzed by an Mx3000P (Agilent Technologies) with SYBR Green Realtime PCR Master Mix (Toyobo). Relative expression levels of the target genes were calculated for each sample after normalization against Gapdh . Statistical analysis was performed using an unpaired t -test and the level of significance was set at P

    Journal: Particle and Fibre Toxicology

    Article Title: Effect of aerosol particles generated by ultrasonic humidifiers on the lung in mouse

    doi: 10.1186/1743-8977-10-64

    Figure Lengend Snippet: Effect of inhalation of particles released from ultrasonic humidifier on mRNA expression of Ccl2 , and Cxcl1 in the lung. Expression levels of Ccl2 and Cxcl1 in the lung tissues (Experiments 3–5, Table 1 ) were analyzed by an Mx3000P (Agilent Technologies) with SYBR Green Realtime PCR Master Mix (Toyobo). Relative expression levels of the target genes were calculated for each sample after normalization against Gapdh . Statistical analysis was performed using an unpaired t -test and the level of significance was set at P

    Article Snippet: Quantitative PCR was performed in a 96-well plate and an Mx3000P (Agilent Technologies) with SYBR Green Realtime PCR Master Mix (Thunderbird; Toyobo Co., Ltd., Osaka, Japan) and specific primers (Fasmac Co. Ltd., Atsugi, Kanagawa, Japan) or with Probe qPCR Master Mix (Thunderbird; Toyobo Co.) and primer (Fasmac)/probe (Biosearch Technologies Japan, Inc., Tokyo, Japan) sets for indicated genes (Additional file : Table S1).

    Techniques: Expressing, SYBR Green Assay, Polymerase Chain Reaction

    Wnt5a , FZD 4, FZD5 and RHOU are significantly down-regulated in newly diagnosed AML patients’ bone marrow samples compared to normal or CR group. Expression levels of Wnt5a , FZD4 , FZD5 and RHOU were determined by RT-PCR analysis of bone marrow samples from AML patients, either newly-diagnosed (AML-ND) or in complete remission (AML-CR), and healthy donors (CON) using SYBR Green PCR Master Mix. The Mann–Whitney test was used to compare differences between the two groups.

    Journal: BMC Cancer

    Article Title: Wnt signaling is involved in 6-benzylthioinosine-induced AML cell differentiation

    doi: 10.1186/1471-2407-14-886

    Figure Lengend Snippet: Wnt5a , FZD 4, FZD5 and RHOU are significantly down-regulated in newly diagnosed AML patients’ bone marrow samples compared to normal or CR group. Expression levels of Wnt5a , FZD4 , FZD5 and RHOU were determined by RT-PCR analysis of bone marrow samples from AML patients, either newly-diagnosed (AML-ND) or in complete remission (AML-CR), and healthy donors (CON) using SYBR Green PCR Master Mix. The Mann–Whitney test was used to compare differences between the two groups.

    Article Snippet: For patient samples, qRT-PCR was performed using SYBR Green PCR Master Mix (Toyobo) on an ABI Prism 7500 sequence detection system.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, SYBR Green Assay, Polymerase Chain Reaction, MANN-WHITNEY

    Linearity of the BCR-ABL ddPCR method when measuring ERM-AD623 samples within a PCR copy number concentration range of 2.5 cp/μL to 5400 cp/μL. The data points represent the average result for eight replicate measurements, and the vertical error bars represent the associated STD. The horizontal error bars represent the standard uncertainty associated with the certified values of the ERM-AD623 samples.

    Journal: Biomolecular Detection and Quantification

    Article Title: Validation of a digital PCR method for quantification of DNA copy number concentrations by using a certified reference material

    doi: 10.1016/j.bdq.2016.08.002

    Figure Lengend Snippet: Linearity of the BCR-ABL ddPCR method when measuring ERM-AD623 samples within a PCR copy number concentration range of 2.5 cp/μL to 5400 cp/μL. The data points represent the average result for eight replicate measurements, and the vertical error bars represent the associated STD. The horizontal error bars represent the standard uncertainty associated with the certified values of the ERM-AD623 samples.

    Article Snippet: The PCR mix comprised 1 × ddPCR Supermix for Probes (Bio-Rad, cat no. 186-3010), suitable primers and probes, nuclease free water (Promega, cat no. P1193) and the DNA sample.

    Techniques: Polymerase Chain Reaction, Concentration Assay

    A schematic overview of all factors which may contribute to the uncertainty of the measurement results obtained with BCR-ABL ddPCR method as performed in this validation study. C primers/probe : concentration primers and probe, Df sample : dilution factor of sample before addition to PCR mix, Df PCR : dilution factor of sample in the PCR mix, M dil : mass of diluent, M dil+sample : mass of diluent and sample, M premix : mass of pre sample mix, M mix : mass of the PCR mix with sample, V d : volume of the droplets

    Journal: Biomolecular Detection and Quantification

    Article Title: Validation of a digital PCR method for quantification of DNA copy number concentrations by using a certified reference material

    doi: 10.1016/j.bdq.2016.08.002

    Figure Lengend Snippet: A schematic overview of all factors which may contribute to the uncertainty of the measurement results obtained with BCR-ABL ddPCR method as performed in this validation study. C primers/probe : concentration primers and probe, Df sample : dilution factor of sample before addition to PCR mix, Df PCR : dilution factor of sample in the PCR mix, M dil : mass of diluent, M dil+sample : mass of diluent and sample, M premix : mass of pre sample mix, M mix : mass of the PCR mix with sample, V d : volume of the droplets

    Article Snippet: The PCR mix comprised 1 × ddPCR Supermix for Probes (Bio-Rad, cat no. 186-3010), suitable primers and probes, nuclease free water (Promega, cat no. P1193) and the DNA sample.

    Techniques: Concentration Assay, Polymerase Chain Reaction

    The relative STD caused by stochastic effects in relation to the PCR copy number concentration for the ddPCR system. The relative STD resulting from stochastic effects ( s s t o c h a s t i c e f f e c t s , r e l ) consists of two components: the relative STD caused by the stochastic effects when sampling the DNA solution added to the PCR mix ( s s a m p l i n g , r e l ) and the relative standard deviation caused by the stochastic effects of the distribution of the target sequence over the analysed droplets ( s d i s t r i b u t i o n , r e l ). The estimation of the s s a m p l i n g , r e l is based on the Poisson distribution, and the estimation of s d i s t r i b u t i o n , r e l is based on the binominal distribution as described in [29] . T s a m p l e d : the expected number of target sequences sampled, T A : the expected number of target sequences in the analysed droplets, A: number of analysed droplets, P: number of positive droplets.

    Journal: Biomolecular Detection and Quantification

    Article Title: Validation of a digital PCR method for quantification of DNA copy number concentrations by using a certified reference material

    doi: 10.1016/j.bdq.2016.08.002

    Figure Lengend Snippet: The relative STD caused by stochastic effects in relation to the PCR copy number concentration for the ddPCR system. The relative STD resulting from stochastic effects ( s s t o c h a s t i c e f f e c t s , r e l ) consists of two components: the relative STD caused by the stochastic effects when sampling the DNA solution added to the PCR mix ( s s a m p l i n g , r e l ) and the relative standard deviation caused by the stochastic effects of the distribution of the target sequence over the analysed droplets ( s d i s t r i b u t i o n , r e l ). The estimation of the s s a m p l i n g , r e l is based on the Poisson distribution, and the estimation of s d i s t r i b u t i o n , r e l is based on the binominal distribution as described in [29] . T s a m p l e d : the expected number of target sequences sampled, T A : the expected number of target sequences in the analysed droplets, A: number of analysed droplets, P: number of positive droplets.

    Article Snippet: The PCR mix comprised 1 × ddPCR Supermix for Probes (Bio-Rad, cat no. 186-3010), suitable primers and probes, nuclease free water (Promega, cat no. P1193) and the DNA sample.

    Techniques: Polymerase Chain Reaction, Concentration Assay, Sampling, Standard Deviation, Sequencing