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  • 87
    Thermo Fisher thermo fisher pcr buffer
    Thermo Fisher Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher polymerase chain reaction pcr rxn buffer
    Polymerase Chain Reaction Pcr Rxn Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Thermo Fisher quantitative reverse transcription polymerase chain reaction pcr trizol solution
    Quantitative Reverse Transcription Polymerase Chain Reaction Pcr Trizol Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher real time polymerase chain reaction taq man technology
    Real Time Polymerase Chain Reaction Taq Man Technology, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 6 article reviews
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    99
    Thermo Fisher pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20465 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 20465 article reviews
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    pcr buffer - by Bioz Stars, 2020-01
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    77
    Thermo Fisher pcr buffer1
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    Pcr Buffer1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 77 stars, based on 3 article reviews
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    77
    Thermo Fisher pcr supermix buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    Pcr Supermix Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr supermix buffer/product/Thermo Fisher
    Average 77 stars, based on 2 article reviews
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    98
    Thermo Fisher supertaq pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    Supertaq Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 38 article reviews
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    83
    Thermo Fisher hifi pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    Hifi Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 83 stars, based on 37 article reviews
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    hifi pcr buffer - by Bioz Stars, 2020-01
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    99
    Thermo Fisher pcr gold buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    Pcr Gold Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 800 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher readymix pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    Readymix Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 5 article reviews
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    99
    Thermo Fisher 1x pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    1x Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher pfx50 pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    Pfx50 Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher 10x pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    10x Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    10x pcr buffer - by Bioz Stars, 2020-01
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    90
    Thermo Fisher taqman pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    Taqman Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 31 article reviews
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    96
    Thermo Fisher pcr amplification buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    70
    Thermo Fisher platinium pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    86
    Thermo Fisher 10ã pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    99
    Thermo Fisher geneamp pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    84
    Thermo Fisher x10 pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    Thermo Fisher pcr buffer 2
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    77
    Thermo Fisher tfi pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    88
    Thermo Fisher pcr optimizer kit
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    78
    Thermo Fisher pcr truestarttm buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    76
    Thermo Fisher dynazyme pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    91
    Thermo Fisher 1× pcr buffer
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    97
    Thermo Fisher pcr reagents
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
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    Thermo Fisher pcr supermix
    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested <t>PCR</t> were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric <t>DNA</t> (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.
    Pcr Supermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested PCR were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric DNA (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.

    Journal: Nucleic Acids Research

    Article Title: High incidence of rapid telomere loss in telomerase-deficient Caenorhabditis elegans

    doi: 10.1093/nar/gkj417

    Figure Lengend Snippet: Structure of telomere fusions isolated from trt-1 . Eight clones of telomere fusions amplified from nested PCR were sequenced and classified into three categories. Category I consists of simple end-to-end fusions. White block arrow denotes the sequence between the inner primer and the beginning of telomeric DNA (represented by the wiggly line). Hatched block arrow denotes sequence between the inner primer and the outer primer. The presence of microhomology or insertion at the fusion junction (shown by an arrow head) is also indicated. In clone 3, 25 telomeric repeats were present at the fusion junction. Category II consists of complicated rearrangements in which a fragment from an internal VL site (denoted by a black block arrow) was inserted between the two ends. In clone 4, the black block arrow represents a 3.8 kb fragment found within the cosmid Y39D8B (∼360 kb from the VL telomere). In clone 5, the black block arrow represents a 0.47 kb fragment found within the cosmid B0348 (∼16 kb from the VL telomere). In clone 6, it represents a 0.73 kb fragment from cosmid B0348 that overlaps with the 0.47 kb fragment inserted in clone 5. The inserted fragments in clones 4–6 all contained imperfect telomeric repeats around the region where it joined the telomeric repeats (represented by wiggly line). In clone 7, the black block arrow represents a 50 bp fragment found in the cosmid C39F7 (1.2 Mb from the VL telomere). This fragment is interspersed with imperfect telomeric repeats. It was inserted in an opposite orientation as the other clones in this category. Category III consists of a clone which involved complicated rearrangement of the VL subtelomeric sequence. Within the fragment between the inner primer and the beginning of telomeric DNA, a 172 bp sequence is replicated 2.5 times (indicated by the division of the block arrow into three parts in gradient) but the replicates carry several mismatches. In clone 8, a sequence (denoted by the small orange block arrow) that overlaps with two of the replicates was inserted between two chromosome ends, one of which was truncated within the 10 bp fragment as in clones 1–3 in category I. Although only the nested primer was used in secondary PCRs, most clones (1–3, 7 and 8) were flanked by the outer primer sequence and the nested primer sequence, indicating that the low concentration of the outer primer in the secondary PCRs was enough to prime amplification with the more abundant nested primer.

    Article Snippet: Primary PCR was carried out in a 20 µl reaction containing half or the whole of the genomic DNA extracted from a single worm, 1× PCR buffer IV (ABgene), 2 mM MgCl2 , 0.1 µM of primer 798 (5′-GGGATGCGCAGCTAACTATAGGAC-3′), 0.3 mM of each dNTP (Amersham) and 1.5 U Extensor Hi-Fidelity PCR Enzyme Mix (ABgene).

    Techniques: Isolation, Clone Assay, Amplification, Nested PCR, Blocking Assay, Sequencing, Concentration Assay

    Short outlying telomeres are readily observed in the DNA from single trt-1 mutants but not in the DNA from five mrt-2 mutants. ( A – D ) Eight to sixteen reproductive-stage individual progeny from four separate trt-1 parents were analyzed by STELA. Each lane represents a single worm. The two clusters of bands in most worms likely represent the two alleles of VL. In order to maintain the strain, trt-1 has to be routinely outcrossed. The longer VL telomere could be derived from the wild-type parent and the shorter VL telomere from the trt-1 parent. ( E ) DNA was extracted from five worms from the same mrt-2 parent in each of F2 (lane 1), F5 (lane 2) and F9 (lane 3) and subsequently used in STELA. A DNA equivalent of on e worm was used as the template in each PCR.

    Journal: Nucleic Acids Research

    Article Title: High incidence of rapid telomere loss in telomerase-deficient Caenorhabditis elegans

    doi: 10.1093/nar/gkj417

    Figure Lengend Snippet: Short outlying telomeres are readily observed in the DNA from single trt-1 mutants but not in the DNA from five mrt-2 mutants. ( A – D ) Eight to sixteen reproductive-stage individual progeny from four separate trt-1 parents were analyzed by STELA. Each lane represents a single worm. The two clusters of bands in most worms likely represent the two alleles of VL. In order to maintain the strain, trt-1 has to be routinely outcrossed. The longer VL telomere could be derived from the wild-type parent and the shorter VL telomere from the trt-1 parent. ( E ) DNA was extracted from five worms from the same mrt-2 parent in each of F2 (lane 1), F5 (lane 2) and F9 (lane 3) and subsequently used in STELA. A DNA equivalent of on e worm was used as the template in each PCR.

    Article Snippet: Primary PCR was carried out in a 20 µl reaction containing half or the whole of the genomic DNA extracted from a single worm, 1× PCR buffer IV (ABgene), 2 mM MgCl2 , 0.1 µM of primer 798 (5′-GGGATGCGCAGCTAACTATAGGAC-3′), 0.3 mM of each dNTP (Amersham) and 1.5 U Extensor Hi-Fidelity PCR Enzyme Mix (ABgene).

    Techniques: Derivative Assay, Polymerase Chain Reaction

    Telomere length heterogeneity in wild-type C.elegans . ( A ) Telomere length fluctuations in the wild-type strain N2. Telomere length of VL was measured by STELA at F1, F5, F9, F13 and F17. DNA was extracted from five reproductive-stage adult sampled at each of the generations. DNA was ligated to the telorette and 0.1 worm equivalent was used in each PCR. Marker lane is shown on the left and the corresponding telomere length is indicated on the right. Actual telomere length was 1.1 kb shorter than the size of the PCR product because 1.1 kb of subtelomeric sequences were also amplified. ( B ) Telomere length heterogeneity in a clonal population. A N2 parent and 10 of its progeny were analyzed by STELA. DNA was extracted from each single worm, ligated to telorette, and the entire DNA sample was used as template in PCR. Each lane represents a single worm.

    Journal: Nucleic Acids Research

    Article Title: High incidence of rapid telomere loss in telomerase-deficient Caenorhabditis elegans

    doi: 10.1093/nar/gkj417

    Figure Lengend Snippet: Telomere length heterogeneity in wild-type C.elegans . ( A ) Telomere length fluctuations in the wild-type strain N2. Telomere length of VL was measured by STELA at F1, F5, F9, F13 and F17. DNA was extracted from five reproductive-stage adult sampled at each of the generations. DNA was ligated to the telorette and 0.1 worm equivalent was used in each PCR. Marker lane is shown on the left and the corresponding telomere length is indicated on the right. Actual telomere length was 1.1 kb shorter than the size of the PCR product because 1.1 kb of subtelomeric sequences were also amplified. ( B ) Telomere length heterogeneity in a clonal population. A N2 parent and 10 of its progeny were analyzed by STELA. DNA was extracted from each single worm, ligated to telorette, and the entire DNA sample was used as template in PCR. Each lane represents a single worm.

    Article Snippet: Primary PCR was carried out in a 20 µl reaction containing half or the whole of the genomic DNA extracted from a single worm, 1× PCR buffer IV (ABgene), 2 mM MgCl2 , 0.1 µM of primer 798 (5′-GGGATGCGCAGCTAACTATAGGAC-3′), 0.3 mM of each dNTP (Amersham) and 1.5 U Extensor Hi-Fidelity PCR Enzyme Mix (ABgene).

    Techniques: Polymerase Chain Reaction, Marker, Amplification

    The mouse H19 upstream regulatory region is biallelically transcribed. ( A ) Schematic representation of the mouse H19 locus and the PCR amplicons used in reverse transcription reactions. The imprinting control region (ICR) extends from −4 to −2 kb in relation to the H19 gene transcription start site, overlapping with the silencer (−2.9 to −1.7 kb relative to the H19 transcription start site). PCR products shown below were amplified from complementary DNA obtained from an F1 generation fetal liver (embryonic day (E)15.5) of a C57Bl6/SD7 cross using random primers, with or without reverse transcriptase (RT). ( B ) Selected sequence traces of PCR products shown in ( A ). Asterisks indicate SNPs between the C57Bl6 and SD7 strains. ( C ) Pol II occupancy at the H19 region. Real-time PCR of ChIP material obtained with an antibody against Pol II or a control antibody. Sequences correspond to amplicons 2 (upstream ICR), 5 ( H19 silencer) and 11 ( H19 gene), and a control fragment from the α-globin gene. ( D ) Analysis of the subcellular localization of the ICR transcripts. RNA from mouse embryonic fibroblasts was fractionated and reverse transcription reactions were carried out on the nuclear and the cytoplasmic RNA pools, respectively. ChIP, chromatin immunoprecipitation; Pol II, RNA polymerase II; SNP, single-nucleotide polymorphism.

    Journal: EMBO Reports

    Article Title: Non-coding transcripts in the H19 imprinting control region mediate gene silencing in transgenic Drosophila

    doi: 10.1038/sj.embor.7401094

    Figure Lengend Snippet: The mouse H19 upstream regulatory region is biallelically transcribed. ( A ) Schematic representation of the mouse H19 locus and the PCR amplicons used in reverse transcription reactions. The imprinting control region (ICR) extends from −4 to −2 kb in relation to the H19 gene transcription start site, overlapping with the silencer (−2.9 to −1.7 kb relative to the H19 transcription start site). PCR products shown below were amplified from complementary DNA obtained from an F1 generation fetal liver (embryonic day (E)15.5) of a C57Bl6/SD7 cross using random primers, with or without reverse transcriptase (RT). ( B ) Selected sequence traces of PCR products shown in ( A ). Asterisks indicate SNPs between the C57Bl6 and SD7 strains. ( C ) Pol II occupancy at the H19 region. Real-time PCR of ChIP material obtained with an antibody against Pol II or a control antibody. Sequences correspond to amplicons 2 (upstream ICR), 5 ( H19 silencer) and 11 ( H19 gene), and a control fragment from the α-globin gene. ( D ) Analysis of the subcellular localization of the ICR transcripts. RNA from mouse embryonic fibroblasts was fractionated and reverse transcription reactions were carried out on the nuclear and the cytoplasmic RNA pools, respectively. ChIP, chromatin immunoprecipitation; Pol II, RNA polymerase II; SNP, single-nucleotide polymorphism.

    Article Snippet: PCRs were carried out with Taq DNA polymerase and PCR optimizer buffers (Invitrogen).

    Techniques: Polymerase Chain Reaction, Amplification, Sequencing, Real-time Polymerase Chain Reaction, Chromatin Immunoprecipitation