pcr buffer Search Results


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  • 99
    New England Biolabs phusion high fidelity pcr master mix
    Phusion High Fidelity Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5052 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr buffer
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28924 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 1x pcr buffer
    1x Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3634 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pcr buffer
    Pcr Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 7741 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 10x pcr buffer
    10x Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore pcr buffer
    Pcr Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 2561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pcr buffer
    Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 10602 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr buffer ii
    Inactivation of <t>MVA-VP2</t> by heat or UV irradiation . Agarose gel electrophoresis of <t>RT-PCR</t> product of the AHSV-VP2 gene transcripts. DF-1 cells were infected with live, purified, heat or UV irradiated MVA-VP2 and 48 h later, VP2-mRNA detection was performed by RT-PCR.
    Pcr Buffer Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2070 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr reaction buffer
    Inactivation of <t>MVA-VP2</t> by heat or UV irradiation . Agarose gel electrophoresis of <t>RT-PCR</t> product of the AHSV-VP2 gene transcripts. DF-1 cells were infected with live, purified, heat or UV irradiated MVA-VP2 and 48 h later, VP2-mRNA detection was performed by RT-PCR.
    Pcr Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5862 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega nested pcr amplification
    Inactivation of <t>MVA-VP2</t> by heat or UV irradiation . Agarose gel electrophoresis of <t>RT-PCR</t> product of the AHSV-VP2 gene transcripts. DF-1 cells were infected with live, purified, heat or UV irradiated MVA-VP2 and 48 h later, VP2-mRNA detection was performed by RT-PCR.
    Nested Pcr Amplification, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meridian Life Science pcr buffer
    Intragenic methylation of PolgA in reprogrammed and mtDNA divergent ESCs. Bisulphite sequencing analysis of CpG methylation in ( A ) CC9 mus , ( B ) CC9 dunni , ( C ) iPS QS , ( D ) iPS NGFP2 ( E ) NT-ES, ( F ) iPS NFGPinj pluripotent stem cells. ( G ) The percentage CpG methylation per sequence determined by bisulphite sequencing (percentage mean ± SEM). ( H ) Real time <t>PCR</t> quantification of PolgA expression in cultured CC9 mus , CC9 spretus , CC9 dunni , iPS QS/R26 (iPS QS ), iPS NGFP , NT-ES, iPS NGFPin j pluripotent stem cells expressed relative to ESD3 cells. ( J ) Cumulative analysis of the relationship between <t>DNA</t> methylation levels ( Figures 1 L and 3 G) and the expression of PolgA ( Figures 2 A and 3 H) performed using Pearson correlation coefficient (R 2 ). ( K ) MtDNA copies/cell in cultured CC9 mus , CC9 spretus , CC9 dunni , iPS QS/R26 , iPS NGFP , NT-ES, iPS NGFPinj pluripotent stem cells. Values represent mean ± SEM and significant differences between cell types are: * P
    Pcr Buffer, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 94/100, based on 2148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr gold buffer
    Intragenic methylation of PolgA in reprogrammed and mtDNA divergent ESCs. Bisulphite sequencing analysis of CpG methylation in ( A ) CC9 mus , ( B ) CC9 dunni , ( C ) iPS QS , ( D ) iPS NGFP2 ( E ) NT-ES, ( F ) iPS NFGPinj pluripotent stem cells. ( G ) The percentage CpG methylation per sequence determined by bisulphite sequencing (percentage mean ± SEM). ( H ) Real time <t>PCR</t> quantification of PolgA expression in cultured CC9 mus , CC9 spretus , CC9 dunni , iPS QS/R26 (iPS QS ), iPS NGFP , NT-ES, iPS NGFPin j pluripotent stem cells expressed relative to ESD3 cells. ( J ) Cumulative analysis of the relationship between <t>DNA</t> methylation levels ( Figures 1 L and 3 G) and the expression of PolgA ( Figures 2 A and 3 H) performed using Pearson correlation coefficient (R 2 ). ( K ) MtDNA copies/cell in cultured CC9 mus , CC9 spretus , CC9 dunni , iPS QS/R26 , iPS NGFP , NT-ES, iPS NGFPinj pluripotent stem cells. Values represent mean ± SEM and significant differences between cell types are: * P
    Pcr Gold Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pre amplification
    Intragenic methylation of PolgA in reprogrammed and mtDNA divergent ESCs. Bisulphite sequencing analysis of CpG methylation in ( A ) CC9 mus , ( B ) CC9 dunni , ( C ) iPS QS , ( D ) iPS NGFP2 ( E ) NT-ES, ( F ) iPS NFGPinj pluripotent stem cells. ( G ) The percentage CpG methylation per sequence determined by bisulphite sequencing (percentage mean ± SEM). ( H ) Real time <t>PCR</t> quantification of PolgA expression in cultured CC9 mus , CC9 spretus , CC9 dunni , iPS QS/R26 (iPS QS ), iPS NGFP , NT-ES, iPS NGFPin j pluripotent stem cells expressed relative to ESD3 cells. ( J ) Cumulative analysis of the relationship between <t>DNA</t> methylation levels ( Figures 1 L and 3 G) and the expression of PolgA ( Figures 2 A and 3 H) performed using Pearson correlation coefficient (R 2 ). ( K ) MtDNA copies/cell in cultured CC9 mus , CC9 spretus , CC9 dunni , iPS QS/R26 , iPS NGFP , NT-ES, iPS NGFPinj pluripotent stem cells. Values represent mean ± SEM and significant differences between cell types are: * P
    Pre Amplification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1965 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim pcr buffer
    Restriction analysis with Rsa I of <t>DNA</t> amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea <t>PCR-1</t> (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.
    Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega 1x pcr buffer
    Restriction analysis with Rsa I of <t>DNA</t> amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea <t>PCR-1</t> (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.
    1x Pcr Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1017 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher geneamp pcr buffer
    Restriction analysis with Rsa I of <t>DNA</t> amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea <t>PCR-1</t> (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.
    Geneamp Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 276 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher accuprime pcr buffer ii
    Restriction analysis with Rsa I of <t>DNA</t> amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea <t>PCR-1</t> (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.
    Accuprime Pcr Buffer Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 1x pcr buffer
    Restriction analysis with Rsa I of <t>DNA</t> amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea <t>PCR-1</t> (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.
    1x Pcr Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher geneamp pcr buffer ii
    Restriction analysis with Rsa I of <t>DNA</t> amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea <t>PCR-1</t> (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.
    Geneamp Pcr Buffer Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 368 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inactivation of MVA-VP2 by heat or UV irradiation . Agarose gel electrophoresis of RT-PCR product of the AHSV-VP2 gene transcripts. DF-1 cells were infected with live, purified, heat or UV irradiated MVA-VP2 and 48 h later, VP2-mRNA detection was performed by RT-PCR.

    Journal: Antiviral Research

    Article Title: The immunogenicity of recombinant vaccines based on modified Vaccinia Ankara (MVA) viruses expressing African horse sickness virus VP2 antigens depends on the levels of expressed VP2 protein delivered to the host

    doi: 10.1016/j.antiviral.2018.04.015

    Figure Lengend Snippet: Inactivation of MVA-VP2 by heat or UV irradiation . Agarose gel electrophoresis of RT-PCR product of the AHSV-VP2 gene transcripts. DF-1 cells were infected with live, purified, heat or UV irradiated MVA-VP2 and 48 h later, VP2-mRNA detection was performed by RT-PCR.

    Article Snippet: Amplification of the VP2 gene was performed by PCR using PCR Buffer II (Invitrogen), dNTPs, specific primers (forward and reverse primers), MgCl2 solution, AmpliTaq DNA Polymerase (Invitrogen), and cDNA template.

    Techniques: Irradiation, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Infection, Purification

    Intragenic methylation of PolgA in reprogrammed and mtDNA divergent ESCs. Bisulphite sequencing analysis of CpG methylation in ( A ) CC9 mus , ( B ) CC9 dunni , ( C ) iPS QS , ( D ) iPS NGFP2 ( E ) NT-ES, ( F ) iPS NFGPinj pluripotent stem cells. ( G ) The percentage CpG methylation per sequence determined by bisulphite sequencing (percentage mean ± SEM). ( H ) Real time PCR quantification of PolgA expression in cultured CC9 mus , CC9 spretus , CC9 dunni , iPS QS/R26 (iPS QS ), iPS NGFP , NT-ES, iPS NGFPin j pluripotent stem cells expressed relative to ESD3 cells. ( J ) Cumulative analysis of the relationship between DNA methylation levels ( Figures 1 L and 3 G) and the expression of PolgA ( Figures 2 A and 3 H) performed using Pearson correlation coefficient (R 2 ). ( K ) MtDNA copies/cell in cultured CC9 mus , CC9 spretus , CC9 dunni , iPS QS/R26 , iPS NGFP , NT-ES, iPS NGFPinj pluripotent stem cells. Values represent mean ± SEM and significant differences between cell types are: * P

    Journal: Nucleic Acids Research

    Article Title: Mitochondrial DNA copy number is regulated in a tissue specific manner by DNA methylation of the nuclear-encoded DNA polymerase gamma A

    doi: 10.1093/nar/gks770

    Figure Lengend Snippet: Intragenic methylation of PolgA in reprogrammed and mtDNA divergent ESCs. Bisulphite sequencing analysis of CpG methylation in ( A ) CC9 mus , ( B ) CC9 dunni , ( C ) iPS QS , ( D ) iPS NGFP2 ( E ) NT-ES, ( F ) iPS NFGPinj pluripotent stem cells. ( G ) The percentage CpG methylation per sequence determined by bisulphite sequencing (percentage mean ± SEM). ( H ) Real time PCR quantification of PolgA expression in cultured CC9 mus , CC9 spretus , CC9 dunni , iPS QS/R26 (iPS QS ), iPS NGFP , NT-ES, iPS NGFPin j pluripotent stem cells expressed relative to ESD3 cells. ( J ) Cumulative analysis of the relationship between DNA methylation levels ( Figures 1 L and 3 G) and the expression of PolgA ( Figures 2 A and 3 H) performed using Pearson correlation coefficient (R 2 ). ( K ) MtDNA copies/cell in cultured CC9 mus , CC9 spretus , CC9 dunni , iPS QS/R26 , iPS NGFP , NT-ES, iPS NGFPinj pluripotent stem cells. Values represent mean ± SEM and significant differences between cell types are: * P

    Article Snippet: Breifly, 200 ng of cDNA or DNA were amplified in 50 µl reactions using 5× PCR buffer (Bioline), 1.5 mM MgCl2 (Bioline), 100 µM dNTPs (Bioline), 0.5 µM for each of the forward and reverse primers.

    Techniques: Methylation, Bisulfite Sequencing, CpG Methylation Assay, Sequencing, Real-time Polymerase Chain Reaction, Expressing, Cell Culture, DNA Methylation Assay

    DNA methylation at the Exon 2 loci is associated with reduced RNApII transcriptional elongation. ( A ) Diagrammatic representation of the PolgA gene and primers sites used for ChIP. Numbers correspond to the centre nucleotide of each primer amplicon, relative to the transcription start site (TSS). Enrichment for (RNApII) and RNApII phosphorylated on serine 2 of the carboxy-terminal domain (RNApIIS2) was analysed by real time PCR at the exon 2 methylation site of PolgA and at downstream and upstream regions in: ( B ) CC9 mus ; ( C ) MEF; ( D ) NSC-CC9 mus and ( E ) heart samples. Values represent mean ± SEM. Significant differences between cell types are: * P

    Journal: Nucleic Acids Research

    Article Title: Mitochondrial DNA copy number is regulated in a tissue specific manner by DNA methylation of the nuclear-encoded DNA polymerase gamma A

    doi: 10.1093/nar/gks770

    Figure Lengend Snippet: DNA methylation at the Exon 2 loci is associated with reduced RNApII transcriptional elongation. ( A ) Diagrammatic representation of the PolgA gene and primers sites used for ChIP. Numbers correspond to the centre nucleotide of each primer amplicon, relative to the transcription start site (TSS). Enrichment for (RNApII) and RNApII phosphorylated on serine 2 of the carboxy-terminal domain (RNApIIS2) was analysed by real time PCR at the exon 2 methylation site of PolgA and at downstream and upstream regions in: ( B ) CC9 mus ; ( C ) MEF; ( D ) NSC-CC9 mus and ( E ) heart samples. Values represent mean ± SEM. Significant differences between cell types are: * P

    Article Snippet: Breifly, 200 ng of cDNA or DNA were amplified in 50 µl reactions using 5× PCR buffer (Bioline), 1.5 mM MgCl2 (Bioline), 100 µM dNTPs (Bioline), 0.5 µM for each of the forward and reverse primers.

    Techniques: DNA Methylation Assay, Chromatin Immunoprecipitation, Amplification, Real-time Polymerase Chain Reaction, Methylation

    Analysis of PolgA and mtDNA enrichment in 5mC and 5hmC MeDIP of ESCs and somatic tissues. DNA samples from cultured CC9 mus , CC9 spretus and CC9 dunni cells; liver, spleen, heart and brain samples were immunoprecipitated using antibodies against ( A ) 5mC and ( B ) 5hmC, and analysed using real time PCR for PolgA (exon 2) enrichment. Bars represent means ± SEM. Significant differences between cell types are indicated (** P

    Journal: Nucleic Acids Research

    Article Title: Mitochondrial DNA copy number is regulated in a tissue specific manner by DNA methylation of the nuclear-encoded DNA polymerase gamma A

    doi: 10.1093/nar/gks770

    Figure Lengend Snippet: Analysis of PolgA and mtDNA enrichment in 5mC and 5hmC MeDIP of ESCs and somatic tissues. DNA samples from cultured CC9 mus , CC9 spretus and CC9 dunni cells; liver, spleen, heart and brain samples were immunoprecipitated using antibodies against ( A ) 5mC and ( B ) 5hmC, and analysed using real time PCR for PolgA (exon 2) enrichment. Bars represent means ± SEM. Significant differences between cell types are indicated (** P

    Article Snippet: Breifly, 200 ng of cDNA or DNA were amplified in 50 µl reactions using 5× PCR buffer (Bioline), 1.5 mM MgCl2 (Bioline), 100 µM dNTPs (Bioline), 0.5 µM for each of the forward and reverse primers.

    Techniques: Methylated DNA Immunoprecipitation, Cell Culture, Immunoprecipitation, Real-time Polymerase Chain Reaction

    DNA Methylation of exon 2 correlates with reduced steady state mRNA levels of PolgA . ( A ) Real time PCR quantification of PolgA expression in cultured ESD3 and MEF cells, and in liver, spleen, heart, muscle, kidney and brain samples, expressed relative to ESD3. ( B ) The relationship between DNA methylation levels from Figure 1 L and the corresponding PolgA expression was determined using Pearson correlation coefficient (R 2 ). ( C ) MtDNA copies/cell in cultured ESD3 and MEF cells, and liver, spleen, heart, muscle, kidney and brain, as determined by real time PCR. ( D ) The relationship between the levels of DNA methylation from Figure 1 L and the corresponding mtDNA copies/cell was determined using Pearson correlation coefficient (R 2 ). Values represent mean ± SEM and significant differences between cell types are: * P

    Journal: Nucleic Acids Research

    Article Title: Mitochondrial DNA copy number is regulated in a tissue specific manner by DNA methylation of the nuclear-encoded DNA polymerase gamma A

    doi: 10.1093/nar/gks770

    Figure Lengend Snippet: DNA Methylation of exon 2 correlates with reduced steady state mRNA levels of PolgA . ( A ) Real time PCR quantification of PolgA expression in cultured ESD3 and MEF cells, and in liver, spleen, heart, muscle, kidney and brain samples, expressed relative to ESD3. ( B ) The relationship between DNA methylation levels from Figure 1 L and the corresponding PolgA expression was determined using Pearson correlation coefficient (R 2 ). ( C ) MtDNA copies/cell in cultured ESD3 and MEF cells, and liver, spleen, heart, muscle, kidney and brain, as determined by real time PCR. ( D ) The relationship between the levels of DNA methylation from Figure 1 L and the corresponding mtDNA copies/cell was determined using Pearson correlation coefficient (R 2 ). Values represent mean ± SEM and significant differences between cell types are: * P

    Article Snippet: Breifly, 200 ng of cDNA or DNA were amplified in 50 µl reactions using 5× PCR buffer (Bioline), 1.5 mM MgCl2 (Bioline), 100 µM dNTPs (Bioline), 0.5 µM for each of the forward and reverse primers.

    Techniques: DNA Methylation Assay, Real-time Polymerase Chain Reaction, Expressing, Cell Culture

    Restriction analysis with Rsa I of DNA amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea PCR-1 (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.

    Journal: Applied and Environmental Microbiology

    Article Title: PCR Amplification of Ribosomal DNA for Species Identification in the Plant Pathogen Genus Phytophthora

    doi:

    Figure Lengend Snippet: Restriction analysis with Rsa I of DNA amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea PCR-1 (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.

    Article Snippet: Each reaction tube contained approximately 1 μl of a 1-ng/μl DNA template, 5 μl of 10× PCR buffer (Boehringer Mannheim, Indianapolis, Ind.), 36.6 μl of sterile distilled water, 2 μl (each) of 1.25 mM deoxynucleoside triphosphates (Pharmacia Biotech, Piscataway, N.J.), 2 μl of 10 mM MgCl2 (Sigma, St. Louis, Mo.), 2 μl each of 10 μM forward and reverse primers , and 0.4 μl of Taq (5 U/μl; Boehringer Mannheim).

    Techniques: Amplification, Polymerase Chain Reaction

    Restriction analysis with Hae III of DNA amplified with ITS 5 and ITS 4 from P. palmivora P8 (lane 2), P. erythroseptica 4 (lane 3), P. cryptogea PCR-1 (lane 4), P. citrophthora M86 (lane 5), P. cinnamomi 2302 (lane 6), P. fragariae A-8 (lane 7), P. megasperma NY 412 (lane 8), P. sojae R1 (lane 9), and P. megasperma NY 318 (lane 10). Lanes 1 and 11 contain 100-bp ladders.

    Journal: Applied and Environmental Microbiology

    Article Title: PCR Amplification of Ribosomal DNA for Species Identification in the Plant Pathogen Genus Phytophthora

    doi:

    Figure Lengend Snippet: Restriction analysis with Hae III of DNA amplified with ITS 5 and ITS 4 from P. palmivora P8 (lane 2), P. erythroseptica 4 (lane 3), P. cryptogea PCR-1 (lane 4), P. citrophthora M86 (lane 5), P. cinnamomi 2302 (lane 6), P. fragariae A-8 (lane 7), P. megasperma NY 412 (lane 8), P. sojae R1 (lane 9), and P. megasperma NY 318 (lane 10). Lanes 1 and 11 contain 100-bp ladders.

    Article Snippet: Each reaction tube contained approximately 1 μl of a 1-ng/μl DNA template, 5 μl of 10× PCR buffer (Boehringer Mannheim, Indianapolis, Ind.), 36.6 μl of sterile distilled water, 2 μl (each) of 1.25 mM deoxynucleoside triphosphates (Pharmacia Biotech, Piscataway, N.J.), 2 μl of 10 mM MgCl2 (Sigma, St. Louis, Mo.), 2 μl each of 10 μM forward and reverse primers , and 0.4 μl of Taq (5 U/μl; Boehringer Mannheim).

    Techniques: Amplification, Polymerase Chain Reaction