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  • 99
    Thermo Fisher pcr buffer
    Expression of the mea-1 allele during early seed development. Amplification of MEA-1 ( top ) and ACTIN-11 ( ACT11 ) as a control for cDNA synthesis. <t>RNA</t> was isolated from siliques derived from self-pollinated mea-1 homozygous pistils ( mea × mea ), self-pollinated wild-type pistils (wt × wt), a cross between a homozygous mea-1 female and wild-type male ( mea × wt), and a cross between wild-type female and homozygous mea-1 male (wt × mea ). Primers that specifically amplify the mea-1 allele under these <t>PCR</t> conditions were used for RT–PCR. (M) Marker lane; (G) genomic DNA as a control.
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore pcr buffer a
    Detection of the p73 genotype by polymerase chain reaction with confronting two-pair primers. Lane M: 100-bp <t>DNA</t> ladder; Lanes 1 and 5: GC/GC genotype; Lanes 2, 3 and 6: GC/AT genotype; Lane 4: AT/AT genotype.
    Pcr Buffer A, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10070 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    PerkinElmer polymerase chain reaction pcr buffer
    Differential detection of T. saginata , T. solium , and E. granulossus by multiplex <t>PCR.</t> Samples of genomic DNA (1 ng) from T. saginata (lane 1), T. solium (lane 2), T. taeniformis B (lane 3), T. taeniformis M (lane 4), E. granulosus (lane 5), a calf (lane 6), and a human (lane 7) were amplified by the multiplex PCR based on the PTs7S35F1, PTs7S35F2, and PTs7S35R1 primers derived from the T. saginata genomic sequence <t>HDP2.</t> A negative control without DNA was also included (lane 8). The reactions were carried out as described in Materials and Methods. The amplification products were fractionated on a 2% agarose gel and were stained with ethidium bromide. Promega PCR molecular markers were used (lanes M).
    Polymerase Chain Reaction Pcr Buffer, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega polymerase chain reaction pcr buffer
    Representative <t>PCR</t> enrichment of HEFs. ChIP was performed in HOXA13-FLAG, HOX (−) and HOXD13-FLAG cell lines using anti-FLAG agarose. PCR detection was performed using primers specific to each HEF, NEF1 and NEF2. NEF1 resulted in no detectable product for each cellular sample and NEF2 resulted in product with no detectable difference between the HOXA13-FLAG or HOXD13-FLAG cell lines and the HOX (−) cells. Water was used as a negative PCR control and input ChIP <t>DNA</t> from the HOX (−) cells was used as the positive PCR control.
    Polymerase Chain Reaction Pcr Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche polymerase chain reaction pcr buffer
    Human papillomavirus (HPV)16 type-specific polymerase chain reaction products. Lane 4: 50 bp ladder; lanes 3, 5-8: <t>HPV16</t> (+) ve samples showing specific band size at 116 bp; lane 9: HPV16 (–)ve sample; lane 2: Positive control; lane 1: Negative control
    Polymerase Chain Reaction Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare polymerase chain reaction pcr buffer
    Gel images illustrating the quality of RNA extracts and their suitability for <t>RT-PCR.</t> In each panel the arrows on the left indicate the position(s) of the expected band(s) on the gel. The arrowheads on the right indicate the positions of the 1,636- and 517- or 506-bp fragments of the molecular weight marker. Lane 1, total nucleic acid extract from reference soil; lane 2, total RNA extract from reference (Ref.) soil; lanes 4 to 9, duplicate SC −N RNA extracts (lanes 4 and 5, day 2; lanes 6 and 7, day 3; lanes 8 and 9, day 7); lanes 11 and 12, LC −N liquid culture RNA extracts from day 2; lanes 3 and 10, 1-kb DNA marker. (A) Six microliters (equivalent to RNA from 0.1 g of soil or 0.4 ml of liquid culture) of nucleic acid extract sample (2% agarose). (B) Six-microliter portions of 50-μl bacterial SSU rRNA RT-PCR products (1,070 bp; 1% agarose). Reference soil extracts were used undiluted, and all other samples were diluted to a concentration of 3 ng μl −1 prior to RT. (C) Six-microliter portions of 50-μl bacterial SSU rRNA PCR products used to test for DNA contamination (1% agarose). The reaction mixtures were identical to those shown in panel B except that they did not contain AMV reverse transcriptase in the RT step. (D) Six-microliter portions of 50-μl A. vinelandii <t>nifH</t> RT-PCR products (370 bp; 2% agarose).
    Polymerase Chain Reaction Pcr Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Roche 1x polymerase chain reaction pcr buffer
    Gel images illustrating the quality of RNA extracts and their suitability for <t>RT-PCR.</t> In each panel the arrows on the left indicate the position(s) of the expected band(s) on the gel. The arrowheads on the right indicate the positions of the 1,636- and 517- or 506-bp fragments of the molecular weight marker. Lane 1, total nucleic acid extract from reference soil; lane 2, total RNA extract from reference (Ref.) soil; lanes 4 to 9, duplicate SC −N RNA extracts (lanes 4 and 5, day 2; lanes 6 and 7, day 3; lanes 8 and 9, day 7); lanes 11 and 12, LC −N liquid culture RNA extracts from day 2; lanes 3 and 10, 1-kb DNA marker. (A) Six microliters (equivalent to RNA from 0.1 g of soil or 0.4 ml of liquid culture) of nucleic acid extract sample (2% agarose). (B) Six-microliter portions of 50-μl bacterial SSU rRNA RT-PCR products (1,070 bp; 1% agarose). Reference soil extracts were used undiluted, and all other samples were diluted to a concentration of 3 ng μl −1 prior to RT. (C) Six-microliter portions of 50-μl bacterial SSU rRNA PCR products used to test for DNA contamination (1% agarose). The reaction mixtures were identical to those shown in panel B except that they did not contain AMV reverse transcriptase in the RT step. (D) Six-microliter portions of 50-μl A. vinelandii <t>nifH</t> RT-PCR products (370 bp; 2% agarose).
    1x Polymerase Chain Reaction Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher polymerase chain reaction pcr rxn buffer
    Gel images illustrating the quality of RNA extracts and their suitability for <t>RT-PCR.</t> In each panel the arrows on the left indicate the position(s) of the expected band(s) on the gel. The arrowheads on the right indicate the positions of the 1,636- and 517- or 506-bp fragments of the molecular weight marker. Lane 1, total nucleic acid extract from reference soil; lane 2, total RNA extract from reference (Ref.) soil; lanes 4 to 9, duplicate SC −N RNA extracts (lanes 4 and 5, day 2; lanes 6 and 7, day 3; lanes 8 and 9, day 7); lanes 11 and 12, LC −N liquid culture RNA extracts from day 2; lanes 3 and 10, 1-kb DNA marker. (A) Six microliters (equivalent to RNA from 0.1 g of soil or 0.4 ml of liquid culture) of nucleic acid extract sample (2% agarose). (B) Six-microliter portions of 50-μl bacterial SSU rRNA RT-PCR products (1,070 bp; 1% agarose). Reference soil extracts were used undiluted, and all other samples were diluted to a concentration of 3 ng μl −1 prior to RT. (C) Six-microliter portions of 50-μl bacterial SSU rRNA PCR products used to test for DNA contamination (1% agarose). The reaction mixtures were identical to those shown in panel B except that they did not contain AMV reverse transcriptase in the RT step. (D) Six-microliter portions of 50-μl A. vinelandii <t>nifH</t> RT-PCR products (370 bp; 2% agarose).
    Polymerase Chain Reaction Pcr Rxn Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pcr buffer
    <t>PCR</t> amplification of total <t>DNA</t> of Arabidopsis
    Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Boehringer Mannheim pcr buffer
    Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, <t>10×</t> buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the <t>PCR.</t>
    Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor polymerase chain reaction pcr buffer
    Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, <t>10×</t> buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the <t>PCR.</t>
    Polymerase Chain Reaction Pcr Buffer, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Bioneer Corporation polymerase chain reaction pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Pcr Buffer, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher polymerase chain reaction conditions 1x pcr gold buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Conditions 1x Pcr Gold Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    SolGent polymerase chain reaction pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Pcr Buffer, supplied by SolGent, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    PerkinElmer 1x polymerase chain reaction pcr buffer ii
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    1x Polymerase Chain Reaction Pcr Buffer Ii, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 81/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega polymerase chain reaction pcr amplification buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Pcr Amplification Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Roche 1×polymerase chain reaction pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    1×Polymerase Chain Reaction Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher accuprime polymerase chain reaction buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Accuprime Polymerase Chain Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Thermo Fisher 1x polymerase chain reaction pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    1x Polymerase Chain Reaction Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    TaKaRa polymerase chain reaction pcr buffer 10x
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Pcr Buffer 10x, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    TaKaRa polymerase chain reaction pcr buffer buffer including 2 5 mm of mg2
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Pcr Buffer Buffer Including 2 5 Mm Of Mg2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher reverse transcriptase polymerase chain reaction rt pcr buffers
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Reverse Transcriptase Polymerase Chain Reaction Rt Pcr Buffers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioTek Instruments reverse transcription quantitative polymerase chain reaction rt qpcr rna extraction buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Reverse Transcription Quantitative Polymerase Chain Reaction Rt Qpcr Rna Extraction Buffer, supplied by BioTek Instruments, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher real time polymerase chain reaction taq man technology
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Real Time Polymerase Chain Reaction Taq Man Technology, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore 10x pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    10x Pcr Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Astral Scientific pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Pcr Buffer, supplied by Astral Scientific, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biometra pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Pcr Buffer, supplied by Biometra, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Detection of C A polymorphism in exon 24 of JHDM2A gene. After treatment of <t>PCR</t> product with Eco RV enzyme, both control and infertile samples showed two fragments. It reveals that no sample has polymorphism at predicted site. A) A 1.5% agarose gel was used here for gel electrophoresis. All of the numbers are in bp. 50 bp <t>DNA</t> Marker (lane M), digested PCR product of fertile sample (lane 1), un-digested PCR product of fertile sample (lane 2), digested PCR product of infertile sample (lane 3), un-digested PCR product of infertile sample (lane 4). B) For detecting the 30 bp band, ethidium bromide staining was performed for more 30 min
    Pcr Buffer, supplied by CinnaGen Co, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Detection of C A polymorphism in exon 24 of JHDM2A gene. After treatment of <t>PCR</t> product with Eco RV enzyme, both control and infertile samples showed two fragments. It reveals that no sample has polymorphism at predicted site. A) A 1.5% agarose gel was used here for gel electrophoresis. All of the numbers are in bp. 50 bp <t>DNA</t> Marker (lane M), digested PCR product of fertile sample (lane 1), un-digested PCR product of fertile sample (lane 2), digested PCR product of infertile sample (lane 3), un-digested PCR product of infertile sample (lane 4). B) For detecting the 30 bp band, ethidium bromide staining was performed for more 30 min
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    Fisher Scientific pcr buffer
    Detection of C A polymorphism in exon 24 of JHDM2A gene. After treatment of <t>PCR</t> product with Eco RV enzyme, both control and infertile samples showed two fragments. It reveals that no sample has polymorphism at predicted site. A) A 1.5% agarose gel was used here for gel electrophoresis. All of the numbers are in bp. 50 bp <t>DNA</t> Marker (lane M), digested PCR product of fertile sample (lane 1), un-digested PCR product of fertile sample (lane 2), digested PCR product of infertile sample (lane 3), un-digested PCR product of infertile sample (lane 4). B) For detecting the 30 bp band, ethidium bromide staining was performed for more 30 min
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    Image Search Results


    Expression of the mea-1 allele during early seed development. Amplification of MEA-1 ( top ) and ACTIN-11 ( ACT11 ) as a control for cDNA synthesis. RNA was isolated from siliques derived from self-pollinated mea-1 homozygous pistils ( mea × mea ), self-pollinated wild-type pistils (wt × wt), a cross between a homozygous mea-1 female and wild-type male ( mea × wt), and a cross between wild-type female and homozygous mea-1 male (wt × mea ). Primers that specifically amplify the mea-1 allele under these PCR conditions were used for RT–PCR. (M) Marker lane; (G) genomic DNA as a control.

    Journal: Genes & Development

    Article Title: Maintenance of genomic imprinting at the Arabidopsis medea locus requires zygotic DDM1 activity

    doi:

    Figure Lengend Snippet: Expression of the mea-1 allele during early seed development. Amplification of MEA-1 ( top ) and ACTIN-11 ( ACT11 ) as a control for cDNA synthesis. RNA was isolated from siliques derived from self-pollinated mea-1 homozygous pistils ( mea × mea ), self-pollinated wild-type pistils (wt × wt), a cross between a homozygous mea-1 female and wild-type male ( mea × wt), and a cross between wild-type female and homozygous mea-1 male (wt × mea ). Primers that specifically amplify the mea-1 allele under these PCR conditions were used for RT–PCR. (M) Marker lane; (G) genomic DNA as a control.

    Article Snippet: For RT–PCR, ∼5 μg of total RNA were treated with 5 units of RNase-free DNase (Boehringer-Mannheim) in 1× PCR buffer (GIBCO-BRL) containing 2.5 m m MgCl2 at 37°C for 30 min. After heat inactivation at 80°C for 5 min, samples were extracted with phenol-chloroform-isoamyl alcohol (25:24:1), and precipitated with ethanol.

    Techniques: Expressing, Microelectrode Array, Amplification, Isolation, Derivative Assay, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Marker

    Detection of the p73 genotype by polymerase chain reaction with confronting two-pair primers. Lane M: 100-bp DNA ladder; Lanes 1 and 5: GC/GC genotype; Lanes 2, 3 and 6: GC/AT genotype; Lane 4: AT/AT genotype.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: p73 G4C14 to A4T14 polymorphism is associated with colorectal cancer risk and survival

    doi: 10.3748/wjg.v16.i35.4448

    Figure Lengend Snippet: Detection of the p73 genotype by polymerase chain reaction with confronting two-pair primers. Lane M: 100-bp DNA ladder; Lanes 1 and 5: GC/GC genotype; Lanes 2, 3 and 6: GC/AT genotype; Lane 4: AT/AT genotype.

    Article Snippet: PCR was performed in a volume of 20 μL containing 100 ng of DNA template, 0.2 mmol/L each deoxynucleotide triphosphate, 1 × PCR buffer (50 mmol/L KCl, 10 mmol/L Tris HCl, and 0.1% Triton X-100), 1.5 mmol/L MgCl2 , 1 U of Taq polymerase (Sigma-Aldrich Biotechnology) and 10 pmol of each of four primers.

    Techniques: Polymerase Chain Reaction

    PCR for the ITS2 region of root DNA samples of family Asparagaceae ( Asparagus recemosus and Asparagus gonoclados ) and Asclepiadaceae ( Hemidesmus indicus and Decalepis hamiltonii ) Detection of the ITS2 region of ~500 bp PCR product on a 1.5% agarose gel. Lane 1. Asparagus racemosus , Lane 2. Asparagus gonoclados , Lane 3. Hemidesmus indicus , Lane 4. Decalepis hamiltonii , Lane 5.100 bp ladder, Lane 6. Negative control

    Journal: Journal of Ayurveda and Integrative Medicine

    Article Title: DNA barcoding of authentic and substitute samples of herb of the family Asparagaceae and Asclepiadaceae based on the ITS2 region

    doi: 10.4103/0975-9476.100177

    Figure Lengend Snippet: PCR for the ITS2 region of root DNA samples of family Asparagaceae ( Asparagus recemosus and Asparagus gonoclados ) and Asclepiadaceae ( Hemidesmus indicus and Decalepis hamiltonii ) Detection of the ITS2 region of ~500 bp PCR product on a 1.5% agarose gel. Lane 1. Asparagus racemosus , Lane 2. Asparagus gonoclados , Lane 3. Hemidesmus indicus , Lane 4. Decalepis hamiltonii , Lane 5.100 bp ladder, Lane 6. Negative control

    Article Snippet: [ ] PCR amplification was performed in 25 μl reaction mixtures containing approximately 40 ng template DNA, 1 X PCR buffer, 1.5 mM MgCl2 , 0.2 mM of each dNTP, 0.1 μM of each primers (Sigma Proligo and Sigma Life Science, India), and 1.0 U Taq DNA Polymerase (Invitrogen) in a thermal cycler (Eppendorf, Germany).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Negative Control

    Differential detection of T. saginata , T. solium , and E. granulossus by multiplex PCR. Samples of genomic DNA (1 ng) from T. saginata (lane 1), T. solium (lane 2), T. taeniformis B (lane 3), T. taeniformis M (lane 4), E. granulosus (lane 5), a calf (lane 6), and a human (lane 7) were amplified by the multiplex PCR based on the PTs7S35F1, PTs7S35F2, and PTs7S35R1 primers derived from the T. saginata genomic sequence HDP2. A negative control without DNA was also included (lane 8). The reactions were carried out as described in Materials and Methods. The amplification products were fractionated on a 2% agarose gel and were stained with ethidium bromide. Promega PCR molecular markers were used (lanes M).

    Journal: Journal of Clinical Microbiology

    Article Title: Differential Diagnosis of Taenia saginata and Taenia solium Infection by PCR

    doi:

    Figure Lengend Snippet: Differential detection of T. saginata , T. solium , and E. granulossus by multiplex PCR. Samples of genomic DNA (1 ng) from T. saginata (lane 1), T. solium (lane 2), T. taeniformis B (lane 3), T. taeniformis M (lane 4), E. granulosus (lane 5), a calf (lane 6), and a human (lane 7) were amplified by the multiplex PCR based on the PTs7S35F1, PTs7S35F2, and PTs7S35R1 primers derived from the T. saginata genomic sequence HDP2. A negative control without DNA was also included (lane 8). The reactions were carried out as described in Materials and Methods. The amplification products were fractionated on a 2% agarose gel and were stained with ethidium bromide. Promega PCR molecular markers were used (lanes M).

    Article Snippet: Multiplex PCR with HDP2-based primers was performed in a total volume of 25 μl with PCR buffer (PCR buffer I; Perkin-Elmer) and final concentrations of 0.4% glycerol, each deoxynucleoside triphosphate (Pharmacia) at a concentration of 200 mM, 0.5 μM primer PTs7S35F1, 0.5 μM primer PTs7S35F2, 1 mM primer PTs7S35R1, and 2.5 U of Taq polymerase (Perkin-Elmer).

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Amplification, Derivative Assay, Sequencing, Negative Control, Agarose Gel Electrophoresis, Staining

    Representative PCR enrichment of HEFs. ChIP was performed in HOXA13-FLAG, HOX (−) and HOXD13-FLAG cell lines using anti-FLAG agarose. PCR detection was performed using primers specific to each HEF, NEF1 and NEF2. NEF1 resulted in no detectable product for each cellular sample and NEF2 resulted in product with no detectable difference between the HOXA13-FLAG or HOXD13-FLAG cell lines and the HOX (−) cells. Water was used as a negative PCR control and input ChIP DNA from the HOX (−) cells was used as the positive PCR control.

    Journal: Nucleic Acids Research

    Article Title: A genomic approach to the identification and characterization of HOXA13 functional binding elements

    doi: 10.1093/nar/gki979

    Figure Lengend Snippet: Representative PCR enrichment of HEFs. ChIP was performed in HOXA13-FLAG, HOX (−) and HOXD13-FLAG cell lines using anti-FLAG agarose. PCR detection was performed using primers specific to each HEF, NEF1 and NEF2. NEF1 resulted in no detectable product for each cellular sample and NEF2 resulted in product with no detectable difference between the HOXA13-FLAG or HOXD13-FLAG cell lines and the HOX (−) cells. Water was used as a negative PCR control and input ChIP DNA from the HOX (−) cells was used as the positive PCR control.

    Article Snippet: ChIP PCR PCR mixtures contained equivalent volume immunoprecipitated DNA, 10 mM primer pair, 1.5 mM MgCl2 , 0.2 mM each dNTP, 1× PCR buffer (Promega) and 1.25 U Taq DNA polymerase (Promega) in a total volume 25 µl.

    Techniques: Polymerase Chain Reaction, Chromatin Immunoprecipitation

    Candidate HOXA13 binding sites in the Enpp2 upstream region are enriched in HOXA13-FLAG expressing cells. ( A ) Candidate in vivo binding sites for HOXA13 were identified upstream of the mouse Enpp2 translational start methionine (ATG) using in vitro core sequence variations ( 9 , 20 ) and are labeled with their position relative to the ATG. The plot resulting from an analysis using Advanced Pipmaker ( ) shows sequence conservation to the region upstream of the human Enpp2 start codon. The candidate HOXA13 binding sites' locations in the mouse sequence are shown as vertical colored lines. The site conserved between mouse and human is labeled in blue (B). Sites that were found in the mouse sequence but were not fully conserved to human are labeled in red (A and C). Candidate sites that were found in the human sequence but are not fully conserved in mouse are labeled in green. PCR primers were designed around each mouse candidate sites (A–C) as well as one additional sequence within the mouse Enpp2 promoter region without a putative HOXA13 binding motif (D). ( B ) Chromatin was prepared from HOXA13-FLAG expressing and HOX (−) cells and subjected to anti-FLAG ChIP. The DNA recovered from the ChIP experiments was used in PCR for the sites upstream of the Enpp2 mouse promoter (A–D). Reproducible enrichment ( n = 4) of sites ‘A’ and ‘B’ and to a lesser extent ‘C’ was observed in HOXA13-FLAG expressing cells. Site ‘D’ was not detectably enriched between cell lines.

    Journal: Nucleic Acids Research

    Article Title: A genomic approach to the identification and characterization of HOXA13 functional binding elements

    doi: 10.1093/nar/gki979

    Figure Lengend Snippet: Candidate HOXA13 binding sites in the Enpp2 upstream region are enriched in HOXA13-FLAG expressing cells. ( A ) Candidate in vivo binding sites for HOXA13 were identified upstream of the mouse Enpp2 translational start methionine (ATG) using in vitro core sequence variations ( 9 , 20 ) and are labeled with their position relative to the ATG. The plot resulting from an analysis using Advanced Pipmaker ( ) shows sequence conservation to the region upstream of the human Enpp2 start codon. The candidate HOXA13 binding sites' locations in the mouse sequence are shown as vertical colored lines. The site conserved between mouse and human is labeled in blue (B). Sites that were found in the mouse sequence but were not fully conserved to human are labeled in red (A and C). Candidate sites that were found in the human sequence but are not fully conserved in mouse are labeled in green. PCR primers were designed around each mouse candidate sites (A–C) as well as one additional sequence within the mouse Enpp2 promoter region without a putative HOXA13 binding motif (D). ( B ) Chromatin was prepared from HOXA13-FLAG expressing and HOX (−) cells and subjected to anti-FLAG ChIP. The DNA recovered from the ChIP experiments was used in PCR for the sites upstream of the Enpp2 mouse promoter (A–D). Reproducible enrichment ( n = 4) of sites ‘A’ and ‘B’ and to a lesser extent ‘C’ was observed in HOXA13-FLAG expressing cells. Site ‘D’ was not detectably enriched between cell lines.

    Article Snippet: ChIP PCR PCR mixtures contained equivalent volume immunoprecipitated DNA, 10 mM primer pair, 1.5 mM MgCl2 , 0.2 mM each dNTP, 1× PCR buffer (Promega) and 1.25 U Taq DNA polymerase (Promega) in a total volume 25 µl.

    Techniques: Binding Assay, Expressing, In Vivo, In Vitro, Sequencing, Labeling, Polymerase Chain Reaction, Chromatin Immunoprecipitation

    Human papillomavirus (HPV)16 type-specific polymerase chain reaction products. Lane 4: 50 bp ladder; lanes 3, 5-8: HPV16 (+) ve samples showing specific band size at 116 bp; lane 9: HPV16 (–)ve sample; lane 2: Positive control; lane 1: Negative control

    Journal: Journal of Mid-Life Health

    Article Title: Polymerase chain reaction and deoxyribonucleic acid-sequencing based study on distribution of human papillomavirus 16/18 among histopathological types of cervical intra-epithelial neoplasia and primary invasive cervical carcinoma: A scenario in North Bengal, India

    doi: 10.4103/0976-7800.127786

    Figure Lengend Snippet: Human papillomavirus (HPV)16 type-specific polymerase chain reaction products. Lane 4: 50 bp ladder; lanes 3, 5-8: HPV16 (+) ve samples showing specific band size at 116 bp; lane 9: HPV16 (–)ve sample; lane 2: Positive control; lane 1: Negative control

    Article Snippet: HPV TS-PCR The HPV16-specific PCR mixture (20 μl) contained ×10 PCR buffer, 2 mM MgCl2 , 200 μM dNTP, 4 ng primers, 0.5 U FS Taq and 100 ng DNA.

    Techniques: Polymerase Chain Reaction, Positive Control, Negative Control

    Flow chart depicting study design. TS-PCR: Type-specific polymerase chain reaction, HPV: Human papillomavirus, H and E: Hematoxylin and eosin, PAS: Periodic Acid-Schiff

    Journal: Journal of Mid-Life Health

    Article Title: Polymerase chain reaction and deoxyribonucleic acid-sequencing based study on distribution of human papillomavirus 16/18 among histopathological types of cervical intra-epithelial neoplasia and primary invasive cervical carcinoma: A scenario in North Bengal, India

    doi: 10.4103/0976-7800.127786

    Figure Lengend Snippet: Flow chart depicting study design. TS-PCR: Type-specific polymerase chain reaction, HPV: Human papillomavirus, H and E: Hematoxylin and eosin, PAS: Periodic Acid-Schiff

    Article Snippet: HPV TS-PCR The HPV16-specific PCR mixture (20 μl) contained ×10 PCR buffer, 2 mM MgCl2 , 200 μM dNTP, 4 ng primers, 0.5 U FS Taq and 100 ng DNA.

    Techniques: Flow Cytometry, Polymerase Chain Reaction

    Gel images illustrating the quality of RNA extracts and their suitability for RT-PCR. In each panel the arrows on the left indicate the position(s) of the expected band(s) on the gel. The arrowheads on the right indicate the positions of the 1,636- and 517- or 506-bp fragments of the molecular weight marker. Lane 1, total nucleic acid extract from reference soil; lane 2, total RNA extract from reference (Ref.) soil; lanes 4 to 9, duplicate SC −N RNA extracts (lanes 4 and 5, day 2; lanes 6 and 7, day 3; lanes 8 and 9, day 7); lanes 11 and 12, LC −N liquid culture RNA extracts from day 2; lanes 3 and 10, 1-kb DNA marker. (A) Six microliters (equivalent to RNA from 0.1 g of soil or 0.4 ml of liquid culture) of nucleic acid extract sample (2% agarose). (B) Six-microliter portions of 50-μl bacterial SSU rRNA RT-PCR products (1,070 bp; 1% agarose). Reference soil extracts were used undiluted, and all other samples were diluted to a concentration of 3 ng μl −1 prior to RT. (C) Six-microliter portions of 50-μl bacterial SSU rRNA PCR products used to test for DNA contamination (1% agarose). The reaction mixtures were identical to those shown in panel B except that they did not contain AMV reverse transcriptase in the RT step. (D) Six-microliter portions of 50-μl A. vinelandii nifH RT-PCR products (370 bp; 2% agarose).

    Journal: Applied and Environmental Microbiology

    Article Title: mRNA Extraction and Reverse Transcription-PCR Protocol for Detection of nifH Gene Expression by Azotobacter vinelandii in Soil

    doi: 10.1128/AEM.69.4.1928-1935.2003

    Figure Lengend Snippet: Gel images illustrating the quality of RNA extracts and their suitability for RT-PCR. In each panel the arrows on the left indicate the position(s) of the expected band(s) on the gel. The arrowheads on the right indicate the positions of the 1,636- and 517- or 506-bp fragments of the molecular weight marker. Lane 1, total nucleic acid extract from reference soil; lane 2, total RNA extract from reference (Ref.) soil; lanes 4 to 9, duplicate SC −N RNA extracts (lanes 4 and 5, day 2; lanes 6 and 7, day 3; lanes 8 and 9, day 7); lanes 11 and 12, LC −N liquid culture RNA extracts from day 2; lanes 3 and 10, 1-kb DNA marker. (A) Six microliters (equivalent to RNA from 0.1 g of soil or 0.4 ml of liquid culture) of nucleic acid extract sample (2% agarose). (B) Six-microliter portions of 50-μl bacterial SSU rRNA RT-PCR products (1,070 bp; 1% agarose). Reference soil extracts were used undiluted, and all other samples were diluted to a concentration of 3 ng μl −1 prior to RT. (C) Six-microliter portions of 50-μl bacterial SSU rRNA PCR products used to test for DNA contamination (1% agarose). The reaction mixtures were identical to those shown in panel B except that they did not contain AMV reverse transcriptase in the RT step. (D) Six-microliter portions of 50-μl A. vinelandii nifH RT-PCR products (370 bp; 2% agarose).

    Article Snippet: The reaction mixture (final volume, 50 μl) contained 0.8 mM MgCl2 , each deoxynucleoside triphosphate at a concentration of 0.2 mM, 0.2 μM primer nifH-g1-forB, 0.2 μM primer nifH-g1-rev, 5 mg of bovine serum albumin per ml, and 1× PCR buffer (Amersham Biosciences).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Marker, Concentration Assay, Polymerase Chain Reaction

    Relationship of A. vinelandii nifH RT-PCR product intensity quantified from ethidium bromide-stained agarose gels to specific N fixation activity determined by acetylene reduction. (A) Liquid culture treatments. (B) Soil culture treatments. The correlation coefficients of the exponential fitting functions were highly significant ( P = 0.01) for both treatment types.

    Journal: Applied and Environmental Microbiology

    Article Title: mRNA Extraction and Reverse Transcription-PCR Protocol for Detection of nifH Gene Expression by Azotobacter vinelandii in Soil

    doi: 10.1128/AEM.69.4.1928-1935.2003

    Figure Lengend Snippet: Relationship of A. vinelandii nifH RT-PCR product intensity quantified from ethidium bromide-stained agarose gels to specific N fixation activity determined by acetylene reduction. (A) Liquid culture treatments. (B) Soil culture treatments. The correlation coefficients of the exponential fitting functions were highly significant ( P = 0.01) for both treatment types.

    Article Snippet: The reaction mixture (final volume, 50 μl) contained 0.8 mM MgCl2 , each deoxynucleoside triphosphate at a concentration of 0.2 mM, 0.2 μM primer nifH-g1-forB, 0.2 μM primer nifH-g1-rev, 5 mg of bovine serum albumin per ml, and 1× PCR buffer (Amersham Biosciences).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Staining, Activity Assay

    Cell density, nitrogen fixation activity, and intensity of nifH mRNA expression plotted against time. (A) Cell counts for DAPI-stained bacteria in liquid culture and soil. (B) Nitrogen fixation activity determined by the acetylene reduction method with liquid cultures and soil. Upper plot, SC −N and LC −N ; lower plot (reduced scale), SC +N and LC +N . (C) Intensities of nifH mRNA RT-PCR products determined by image analyses of gel images. Left panel, liquid culture treatments; right panel, soil culture treatments. The error bars in panels A and B indicate the standard errors of the measurements. Symbols: ▪, LC +N or SC +N replicate 1; •, LC +N or SC +N replicate 2; □, LC −N or SC −N replicate 1; ○, LC −N or SC −N replicate 2; ×, LC control or SC control. n.d., not determined.

    Journal: Applied and Environmental Microbiology

    Article Title: mRNA Extraction and Reverse Transcription-PCR Protocol for Detection of nifH Gene Expression by Azotobacter vinelandii in Soil

    doi: 10.1128/AEM.69.4.1928-1935.2003

    Figure Lengend Snippet: Cell density, nitrogen fixation activity, and intensity of nifH mRNA expression plotted against time. (A) Cell counts for DAPI-stained bacteria in liquid culture and soil. (B) Nitrogen fixation activity determined by the acetylene reduction method with liquid cultures and soil. Upper plot, SC −N and LC −N ; lower plot (reduced scale), SC +N and LC +N . (C) Intensities of nifH mRNA RT-PCR products determined by image analyses of gel images. Left panel, liquid culture treatments; right panel, soil culture treatments. The error bars in panels A and B indicate the standard errors of the measurements. Symbols: ▪, LC +N or SC +N replicate 1; •, LC +N or SC +N replicate 2; □, LC −N or SC −N replicate 1; ○, LC −N or SC −N replicate 2; ×, LC control or SC control. n.d., not determined.

    Article Snippet: The reaction mixture (final volume, 50 μl) contained 0.8 mM MgCl2 , each deoxynucleoside triphosphate at a concentration of 0.2 mM, 0.2 μM primer nifH-g1-forB, 0.2 μM primer nifH-g1-rev, 5 mg of bovine serum albumin per ml, and 1× PCR buffer (Amersham Biosciences).

    Techniques: Activity Assay, Expressing, Staining, Reverse Transcription Polymerase Chain Reaction

    PCR amplification of total DNA of Arabidopsis

    Journal: Journal of Virology

    Article Title: Widespread Horizontal Gene Transfer from Double-Stranded RNA Viruses to Eukaryotic Nuclear Genomes ▿Widespread Horizontal Gene Transfer from Double-Stranded RNA Viruses to Eukaryotic Nuclear Genomes ▿ †

    doi: 10.1128/JVI.00955-10

    Figure Lengend Snippet: PCR amplification of total DNA of Arabidopsis

    Article Snippet: The total volume of each PCR mix was 20 μl and contained 1 μl of DNA template, 2 μl 10× PCR buffer (TaKaRa), 0.4 μl of deoxynucleoside triphosphate (dNTP) mix (10 mM [each dNTP]), 0.2 μl of each primer (20 μM), and 1 unit of Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Amplification

    Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, 10× buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the PCR.

    Journal: Journal of Clinical Microbiology

    Article Title: Contaminations Occurring in Fungal PCR Assays

    doi:

    Figure Lengend Snippet: Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, 10× buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the PCR.

    Article Snippet: Detailed and repeated serial analysis of all components of the PCR mixture (nucleotides, primers, water, magnesium chloride, 10× buffer, and Taq polymerase) showed a positive specific band only in the 10× PCR buffer supplied by Boehringer Mannheim.

    Techniques: Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, Amplification, Marker, Labeling, Positive Control, Negative Control, Polymerase Chain Reaction

    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated PCR products size of the reference strain was confirmed by DNA sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.

    Journal: Osong Public Health and Research Perspectives

    Article Title: Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients

    doi: 10.1016/j.phrp.2013.09.005

    Figure Lengend Snippet: Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated PCR products size of the reference strain was confirmed by DNA sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.

    Article Snippet: The DNA amplification was performed using 15 μL of solution containing 10 ng of genomic DNA, 1 U of i-Max II DNA polymerase (iNtRON Biotechnology, Gyeonggi-do, Korea), 2.5 mM deoxyribonucleotide triphosphate mixture, 10× polymerase chain reaction (PCR) buffer, 10 pmol of each primer (Bioneer, Daejeon, Korea), 5× betaine (Sigma Aldrich, St. Louis, MO, USA), and distilled sterilized water.

    Techniques: Amplification, Polymerase Chain Reaction, DNA Sequencing

    Detection of C A polymorphism in exon 24 of JHDM2A gene. After treatment of PCR product with Eco RV enzyme, both control and infertile samples showed two fragments. It reveals that no sample has polymorphism at predicted site. A) A 1.5% agarose gel was used here for gel electrophoresis. All of the numbers are in bp. 50 bp DNA Marker (lane M), digested PCR product of fertile sample (lane 1), un-digested PCR product of fertile sample (lane 2), digested PCR product of infertile sample (lane 3), un-digested PCR product of infertile sample (lane 4). B) For detecting the 30 bp band, ethidium bromide staining was performed for more 30 min

    Journal: International Journal of Reproductive Biomedicine

    Article Title: Association between polymorphisms of exon 12 and exon 24 of JHDM2A gene and male infertility

    doi:

    Figure Lengend Snippet: Detection of C A polymorphism in exon 24 of JHDM2A gene. After treatment of PCR product with Eco RV enzyme, both control and infertile samples showed two fragments. It reveals that no sample has polymorphism at predicted site. A) A 1.5% agarose gel was used here for gel electrophoresis. All of the numbers are in bp. 50 bp DNA Marker (lane M), digested PCR product of fertile sample (lane 1), un-digested PCR product of fertile sample (lane 2), digested PCR product of infertile sample (lane 3), un-digested PCR product of infertile sample (lane 4). B) For detecting the 30 bp band, ethidium bromide staining was performed for more 30 min

    Article Snippet: PCR reactions were performed in 25 µl reaction mixture containing 1 µl of template DNA (100 ng/µl), 2.5µl of 10× PCR Buffer (20 mM Tris-HCl pH 8.6, 50 mM KCl, Cinnagen Inc, Iran), 1 µl of MgCl2 (50 mM, Cinnagen Inc, Iran), 0.5 µl of each forward and reverse primer (10 pM/µl), 0.5 µl of dNTPs mix (10 mM, Cinnagen Inc, Iran), 0.3 µl of Taq DNA polymerase (Cinnagene, Co., Iran).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Nucleic Acid Electrophoresis, Marker, Staining

    PCR product of exon 24. A fragment of exon 24 (133 bp) was successfully amplified through PCR reaction. 50 bp DNA Marker (lane M). A 1% agarose gel was used here for gel electrophoresis. All of the numbers are in bp

    Journal: International Journal of Reproductive Biomedicine

    Article Title: Association between polymorphisms of exon 12 and exon 24 of JHDM2A gene and male infertility

    doi:

    Figure Lengend Snippet: PCR product of exon 24. A fragment of exon 24 (133 bp) was successfully amplified through PCR reaction. 50 bp DNA Marker (lane M). A 1% agarose gel was used here for gel electrophoresis. All of the numbers are in bp

    Article Snippet: PCR reactions were performed in 25 µl reaction mixture containing 1 µl of template DNA (100 ng/µl), 2.5µl of 10× PCR Buffer (20 mM Tris-HCl pH 8.6, 50 mM KCl, Cinnagen Inc, Iran), 1 µl of MgCl2 (50 mM, Cinnagen Inc, Iran), 0.5 µl of each forward and reverse primer (10 pM/µl), 0.5 µl of dNTPs mix (10 mM, Cinnagen Inc, Iran), 0.3 µl of Taq DNA polymerase (Cinnagene, Co., Iran).

    Techniques: Polymerase Chain Reaction, Amplification, Marker, Agarose Gel Electrophoresis, Nucleic Acid Electrophoresis

    Polyacrylamide gel after applying SSCP technique for exon 24 PCR products. All single strand bands are in the same position and no polymorphism is detected. 50 bp DNA Marker (lane M), fertile samples (lane 1, 2), infertile samples (lane 3, 4, 5, 6

    Journal: International Journal of Reproductive Biomedicine

    Article Title: Association between polymorphisms of exon 12 and exon 24 of JHDM2A gene and male infertility

    doi:

    Figure Lengend Snippet: Polyacrylamide gel after applying SSCP technique for exon 24 PCR products. All single strand bands are in the same position and no polymorphism is detected. 50 bp DNA Marker (lane M), fertile samples (lane 1, 2), infertile samples (lane 3, 4, 5, 6

    Article Snippet: PCR reactions were performed in 25 µl reaction mixture containing 1 µl of template DNA (100 ng/µl), 2.5µl of 10× PCR Buffer (20 mM Tris-HCl pH 8.6, 50 mM KCl, Cinnagen Inc, Iran), 1 µl of MgCl2 (50 mM, Cinnagen Inc, Iran), 0.5 µl of each forward and reverse primer (10 pM/µl), 0.5 µl of dNTPs mix (10 mM, Cinnagen Inc, Iran), 0.3 µl of Taq DNA polymerase (Cinnagene, Co., Iran).

    Techniques: Polymerase Chain Reaction, Marker

    Amplification of exon 12 using specific primers. A fragment of exon 12 (225 bp) was successfully amplified through PCR reaction. 1 Kb DNA Marker (lane M). A 1% agarose gel was used here for gel electrophoresis. All of the numbers are in bp

    Journal: International Journal of Reproductive Biomedicine

    Article Title: Association between polymorphisms of exon 12 and exon 24 of JHDM2A gene and male infertility

    doi:

    Figure Lengend Snippet: Amplification of exon 12 using specific primers. A fragment of exon 12 (225 bp) was successfully amplified through PCR reaction. 1 Kb DNA Marker (lane M). A 1% agarose gel was used here for gel electrophoresis. All of the numbers are in bp

    Article Snippet: PCR reactions were performed in 25 µl reaction mixture containing 1 µl of template DNA (100 ng/µl), 2.5µl of 10× PCR Buffer (20 mM Tris-HCl pH 8.6, 50 mM KCl, Cinnagen Inc, Iran), 1 µl of MgCl2 (50 mM, Cinnagen Inc, Iran), 0.5 µl of each forward and reverse primer (10 pM/µl), 0.5 µl of dNTPs mix (10 mM, Cinnagen Inc, Iran), 0.3 µl of Taq DNA polymerase (Cinnagene, Co., Iran).

    Techniques: Amplification, Polymerase Chain Reaction, Marker, Agarose Gel Electrophoresis, Nucleic Acid Electrophoresis