pcr buffer Search Results


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  • 99
    Thermo Fisher pcr buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore polymerase chain reaction pcr buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    Polymerase Chain Reaction Pcr Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Roche polymerase chain reaction pcr buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    Polymerase Chain Reaction Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 36 article reviews
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    90
    PerkinElmer polymerase chain reaction pcr buffer
    Differential detection of T. saginata , T. solium , and E. granulossus by multiplex <t>PCR.</t> Samples of genomic DNA (1 ng) from T. saginata (lane 1), T. solium (lane 2), T. taeniformis B (lane 3), T. taeniformis M (lane 4), E. granulosus (lane 5), a calf (lane 6), and a human (lane 7) were amplified by the multiplex PCR based on the PTs7S35F1, PTs7S35F2, and PTs7S35R1 primers derived from the T. saginata genomic sequence <t>HDP2.</t> A negative control without DNA was also included (lane 8). The reactions were carried out as described in Materials and Methods. The amplification products were fractionated on a 2% agarose gel and were stained with ethidium bromide. Promega PCR molecular markers were used (lanes M).
    Polymerase Chain Reaction Pcr Buffer, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    GE Healthcare polymerase chain reaction pcr buffer
    Differential detection of T. saginata , T. solium , and E. granulossus by multiplex <t>PCR.</t> Samples of genomic DNA (1 ng) from T. saginata (lane 1), T. solium (lane 2), T. taeniformis B (lane 3), T. taeniformis M (lane 4), E. granulosus (lane 5), a calf (lane 6), and a human (lane 7) were amplified by the multiplex PCR based on the PTs7S35F1, PTs7S35F2, and PTs7S35R1 primers derived from the T. saginata genomic sequence <t>HDP2.</t> A negative control without DNA was also included (lane 8). The reactions were carried out as described in Materials and Methods. The amplification products were fractionated on a 2% agarose gel and were stained with ethidium bromide. Promega PCR molecular markers were used (lanes M).
    Polymerase Chain Reaction Pcr Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega polymerase chain reaction pcr buffer
    Differential detection of T. saginata , T. solium , and E. granulossus by multiplex <t>PCR.</t> Samples of genomic DNA (1 ng) from T. saginata (lane 1), T. solium (lane 2), T. taeniformis B (lane 3), T. taeniformis M (lane 4), E. granulosus (lane 5), a calf (lane 6), and a human (lane 7) were amplified by the multiplex PCR based on the PTs7S35F1, PTs7S35F2, and PTs7S35R1 primers derived from the T. saginata genomic sequence <t>HDP2.</t> A negative control without DNA was also included (lane 8). The reactions were carried out as described in Materials and Methods. The amplification products were fractionated on a 2% agarose gel and were stained with ethidium bromide. Promega PCR molecular markers were used (lanes M).
    Polymerase Chain Reaction Pcr Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Roche 1x polymerase chain reaction pcr buffer
    Differential detection of T. saginata , T. solium , and E. granulossus by multiplex <t>PCR.</t> Samples of genomic DNA (1 ng) from T. saginata (lane 1), T. solium (lane 2), T. taeniformis B (lane 3), T. taeniformis M (lane 4), E. granulosus (lane 5), a calf (lane 6), and a human (lane 7) were amplified by the multiplex PCR based on the PTs7S35F1, PTs7S35F2, and PTs7S35R1 primers derived from the T. saginata genomic sequence <t>HDP2.</t> A negative control without DNA was also included (lane 8). The reactions were carried out as described in Materials and Methods. The amplification products were fractionated on a 2% agarose gel and were stained with ethidium bromide. Promega PCR molecular markers were used (lanes M).
    1x Polymerase Chain Reaction Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher accuprime polymerase chain reaction buffer
    Differential detection of T. saginata , T. solium , and E. granulossus by multiplex <t>PCR.</t> Samples of genomic DNA (1 ng) from T. saginata (lane 1), T. solium (lane 2), T. taeniformis B (lane 3), T. taeniformis M (lane 4), E. granulosus (lane 5), a calf (lane 6), and a human (lane 7) were amplified by the multiplex PCR based on the PTs7S35F1, PTs7S35F2, and PTs7S35R1 primers derived from the T. saginata genomic sequence <t>HDP2.</t> A negative control without DNA was also included (lane 8). The reactions were carried out as described in Materials and Methods. The amplification products were fractionated on a 2% agarose gel and were stained with ethidium bromide. Promega PCR molecular markers were used (lanes M).
    Accuprime Polymerase Chain Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    TaKaRa polymerase chain reaction pcr buffer
    Multiple strain colonization detected on the basis of HP0527 gene in cag PAI. Lanes 1 to 10, single colonies isolated from the patient; C, Control strain 26695. (a) Three types of colonies were identified in PG93. Lane 1 and 6 gave a higher amplicon than that of 26695; lanes 3, 4, 7 and 8 yielded same amplicon while lanes 2, 5, 9, 10 produced lower amplicon than that of 26695. (b) Mixed infections detected on the basis of vapD genetic locus <t>PCR.</t> M, 100 bp marker; lanes 1–10, single colonies isolated from PG137; 11, positive control (PCR225); 12, Negative control ( E. coli DNA). All the colonies are positive for vapD except colony numbers 1, 6, 9 and 10.
    Polymerase Chain Reaction Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    PerkinElmer 1x polymerase chain reaction pcr buffer ii
    Multiple strain colonization detected on the basis of HP0527 gene in cag PAI. Lanes 1 to 10, single colonies isolated from the patient; C, Control strain 26695. (a) Three types of colonies were identified in PG93. Lane 1 and 6 gave a higher amplicon than that of 26695; lanes 3, 4, 7 and 8 yielded same amplicon while lanes 2, 5, 9, 10 produced lower amplicon than that of 26695. (b) Mixed infections detected on the basis of vapD genetic locus <t>PCR.</t> M, 100 bp marker; lanes 1–10, single colonies isolated from PG137; 11, positive control (PCR225); 12, Negative control ( E. coli DNA). All the colonies are positive for vapD except colony numbers 1, 6, 9 and 10.
    1x Polymerase Chain Reaction Pcr Buffer Ii, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher polymerase chain reaction conditions 1x pcr gold buffer
    Multiple strain colonization detected on the basis of HP0527 gene in cag PAI. Lanes 1 to 10, single colonies isolated from the patient; C, Control strain 26695. (a) Three types of colonies were identified in PG93. Lane 1 and 6 gave a higher amplicon than that of 26695; lanes 3, 4, 7 and 8 yielded same amplicon while lanes 2, 5, 9, 10 produced lower amplicon than that of 26695. (b) Mixed infections detected on the basis of vapD genetic locus <t>PCR.</t> M, 100 bp marker; lanes 1–10, single colonies isolated from PG137; 11, positive control (PCR225); 12, Negative control ( E. coli DNA). All the colonies are positive for vapD except colony numbers 1, 6, 9 and 10.
    Polymerase Chain Reaction Conditions 1x Pcr Gold Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Avantor polymerase chain reaction pcr buffer
    Multiple strain colonization detected on the basis of HP0527 gene in cag PAI. Lanes 1 to 10, single colonies isolated from the patient; C, Control strain 26695. (a) Three types of colonies were identified in PG93. Lane 1 and 6 gave a higher amplicon than that of 26695; lanes 3, 4, 7 and 8 yielded same amplicon while lanes 2, 5, 9, 10 produced lower amplicon than that of 26695. (b) Mixed infections detected on the basis of vapD genetic locus <t>PCR.</t> M, 100 bp marker; lanes 1–10, single colonies isolated from PG137; 11, positive control (PCR225); 12, Negative control ( E. coli DNA). All the colonies are positive for vapD except colony numbers 1, 6, 9 and 10.
    Polymerase Chain Reaction Pcr Buffer, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Bioneer Corporation polymerase chain reaction pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Pcr Buffer, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    SolGent polymerase chain reaction pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Pcr Buffer, supplied by SolGent, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim pcr buffer
    Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, <t>10×</t> buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the <t>PCR.</t>
    Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher polymerase chain reaction pcr buffer ii
    Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, <t>10×</t> buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the <t>PCR.</t>
    Polymerase Chain Reaction Pcr Buffer Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega polymerase chain reaction pcr amplification buffer
    Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, <t>10×</t> buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the <t>PCR.</t>
    Polymerase Chain Reaction Pcr Amplification Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher 1x polymerase chain reaction pcr buffer
    Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, <t>10×</t> buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the <t>PCR.</t>
    1x Polymerase Chain Reaction Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TaKaRa polymerase chain reaction pcr buffer 10x
    Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, <t>10×</t> buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the <t>PCR.</t>
    Polymerase Chain Reaction Pcr Buffer 10x, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Detection of nptII gene in samples (Results of PCR products of primer pairs (nptIIf-nptIIr)) M: 100 bp DNA marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of nptII gene in samples (Results of PCR products of primer pairs (nptIIf-nptIIr)) M: 100 bp DNA marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

    Detection of epsps gene in some samples tested (Results of PCR products of primer pairs (GMO9/GMO5)) M: 100 bp DNA marker, B: negative control, lanes A1–M10: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of epsps gene in some samples tested (Results of PCR products of primer pairs (GMO9/GMO5)) M: 100 bp DNA marker, B: negative control, lanes A1–M10: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of epsps gene in samples (Results of PCR products of primer pairs: GMO5, GMO9) M: 100 bp DNA marker, B: negative control, lanes A1–P2: Tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of epsps gene in samples (Results of PCR products of primer pairs: GMO5, GMO9) M: 100 bp DNA marker, B: negative control, lanes A1–P2: Tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of lectin gene in soybean samples tested (A1, A2, A3, A4, A5, and A6) (Results of PCR products of primer pair (GM03/GM04)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of lectin gene in soybean samples tested (A1, A2, A3, A4, A5, and A6) (Results of PCR products of primer pair (GM03/GM04)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of 35S promoter in samples (Results of PCR products of primer pairs p35S-cf3 and p35S-cf4), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of 35S promoter in samples (Results of PCR products of primer pairs p35S-cf3 and p35S-cf4), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

    Detection of zein gene in maize samples tested (M1, M2, M3, M5, M6, M7, M8, M9, M10, M11, M12, M13, M14, M15, and M16) (Results of PCR products of primer pair zein3/zein4), M: 100 bp DNA marker, B: negative control, lanes A1–P2: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of zein gene in maize samples tested (M1, M2, M3, M5, M6, M7, M8, M9, M10, M11, M12, M13, M14, M15, and M16) (Results of PCR products of primer pair zein3/zein4), M: 100 bp DNA marker, B: negative control, lanes A1–P2: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of nos terminator in samples (Results of PCR products of primer pairs HA-nos118-f/HA-nos118-r), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of nos terminator in samples (Results of PCR products of primer pairs HA-nos118-f/HA-nos118-r), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

    Detection of epsps gene in some samples tested by nested PCR (Results of PCR products of primer pairs (GMO8/GMO7)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of epsps gene in some samples tested by nested PCR (Results of PCR products of primer pairs (GMO8/GMO7)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Nested PCR, Polymerase Chain Reaction, Marker, Negative Control

    Differential detection of T. saginata , T. solium , and E. granulossus by multiplex PCR. Samples of genomic DNA (1 ng) from T. saginata (lane 1), T. solium (lane 2), T. taeniformis B (lane 3), T. taeniformis M (lane 4), E. granulosus (lane 5), a calf (lane 6), and a human (lane 7) were amplified by the multiplex PCR based on the PTs7S35F1, PTs7S35F2, and PTs7S35R1 primers derived from the T. saginata genomic sequence HDP2. A negative control without DNA was also included (lane 8). The reactions were carried out as described in Materials and Methods. The amplification products were fractionated on a 2% agarose gel and were stained with ethidium bromide. Promega PCR molecular markers were used (lanes M).

    Journal: Journal of Clinical Microbiology

    Article Title: Differential Diagnosis of Taenia saginata and Taenia solium Infection by PCR

    doi:

    Figure Lengend Snippet: Differential detection of T. saginata , T. solium , and E. granulossus by multiplex PCR. Samples of genomic DNA (1 ng) from T. saginata (lane 1), T. solium (lane 2), T. taeniformis B (lane 3), T. taeniformis M (lane 4), E. granulosus (lane 5), a calf (lane 6), and a human (lane 7) were amplified by the multiplex PCR based on the PTs7S35F1, PTs7S35F2, and PTs7S35R1 primers derived from the T. saginata genomic sequence HDP2. A negative control without DNA was also included (lane 8). The reactions were carried out as described in Materials and Methods. The amplification products were fractionated on a 2% agarose gel and were stained with ethidium bromide. Promega PCR molecular markers were used (lanes M).

    Article Snippet: Multiplex PCR with HDP2-based primers was performed in a total volume of 25 μl with PCR buffer (PCR buffer I; Perkin-Elmer) and final concentrations of 0.4% glycerol, each deoxynucleoside triphosphate (Pharmacia) at a concentration of 200 mM, 0.5 μM primer PTs7S35F1, 0.5 μM primer PTs7S35F2, 1 mM primer PTs7S35R1, and 2.5 U of Taq polymerase (Perkin-Elmer).

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Amplification, Derivative Assay, Sequencing, Negative Control, Agarose Gel Electrophoresis, Staining

    Multiple strain colonization detected on the basis of HP0527 gene in cag PAI. Lanes 1 to 10, single colonies isolated from the patient; C, Control strain 26695. (a) Three types of colonies were identified in PG93. Lane 1 and 6 gave a higher amplicon than that of 26695; lanes 3, 4, 7 and 8 yielded same amplicon while lanes 2, 5, 9, 10 produced lower amplicon than that of 26695. (b) Mixed infections detected on the basis of vapD genetic locus PCR. M, 100 bp marker; lanes 1–10, single colonies isolated from PG137; 11, positive control (PCR225); 12, Negative control ( E. coli DNA). All the colonies are positive for vapD except colony numbers 1, 6, 9 and 10.

    Journal: PLoS ONE

    Article Title: Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India

    doi: 10.1371/journal.pone.0043370

    Figure Lengend Snippet: Multiple strain colonization detected on the basis of HP0527 gene in cag PAI. Lanes 1 to 10, single colonies isolated from the patient; C, Control strain 26695. (a) Three types of colonies were identified in PG93. Lane 1 and 6 gave a higher amplicon than that of 26695; lanes 3, 4, 7 and 8 yielded same amplicon while lanes 2, 5, 9, 10 produced lower amplicon than that of 26695. (b) Mixed infections detected on the basis of vapD genetic locus PCR. M, 100 bp marker; lanes 1–10, single colonies isolated from PG137; 11, positive control (PCR225); 12, Negative control ( E. coli DNA). All the colonies are positive for vapD except colony numbers 1, 6, 9 and 10.

    Article Snippet: Characterization of Strains by Multiplex PCR Multiplex PCR for the characterization of vacA s1, vacA s2, vacA m1, vacA m2 and cagA was carried out in 25 µl volume containing 2.5 pmol of primers VAG-F and VAG-R, 25 pmol of primers VA1-F and VA1-R, 10 pmol of primers cag5c-F and cag3c-R, 0.25 mM of each deoxynucleoside triphosphate (Takara), 0.9 U of Taq DNA polymerase (Genei, Bangalore, India), and 1.5 mM of MgCl2 in standard PCR buffer (Takara).

    Techniques: Isolation, Amplification, Produced, Polymerase Chain Reaction, Marker, Positive Control, Negative Control

    PG207 showed evidence of mixed infections on the basis of obtaining either iceA1 or iceA2 alleles. M, 100 bp marker; lanes 1–10, single colonies isolated from PG207; C, positive control; N, Negative control ( E. coli DNA). (a) All the single colonies were negative for iceA1 except for lane 8. (b) All these single colonies were positive for iceA2 except for lane 8. Three different amplicon sizes were obtained in this iceA2 PCR (lanes 1, 9; lanes 2, 3, 6, 7, 10 and lanes 4, 5).

    Journal: PLoS ONE

    Article Title: Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India

    doi: 10.1371/journal.pone.0043370

    Figure Lengend Snippet: PG207 showed evidence of mixed infections on the basis of obtaining either iceA1 or iceA2 alleles. M, 100 bp marker; lanes 1–10, single colonies isolated from PG207; C, positive control; N, Negative control ( E. coli DNA). (a) All the single colonies were negative for iceA1 except for lane 8. (b) All these single colonies were positive for iceA2 except for lane 8. Three different amplicon sizes were obtained in this iceA2 PCR (lanes 1, 9; lanes 2, 3, 6, 7, 10 and lanes 4, 5).

    Article Snippet: Characterization of Strains by Multiplex PCR Multiplex PCR for the characterization of vacA s1, vacA s2, vacA m1, vacA m2 and cagA was carried out in 25 µl volume containing 2.5 pmol of primers VAG-F and VAG-R, 25 pmol of primers VA1-F and VA1-R, 10 pmol of primers cag5c-F and cag3c-R, 0.25 mM of each deoxynucleoside triphosphate (Takara), 0.9 U of Taq DNA polymerase (Genei, Bangalore, India), and 1.5 mM of MgCl2 in standard PCR buffer (Takara).

    Techniques: Marker, Isolation, Positive Control, Negative Control, Amplification, Polymerase Chain Reaction

    Multiplex PCR for vacA subtypes and cagA gene for PG218. M, 100 bp marker; lanes 1–10, single colonies isolated from PG218 and “P” denotes pooled DNA sample; C, Positive control (26695); N, Negative control ( E. coli DNA). All the colonies isolated from this individual were cagA + and carried vacA s1 allele. Evidence of mixed infection was detected in the vacA middle region only. Lanes 1–3 and 10 yielded amplicon specific for m1, lanes 5–9 produced amplicon specific for m2 while in lane 4, no amplicon was obtained for vacA mid-region using this specific primer set.

    Journal: PLoS ONE

    Article Title: Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India

    doi: 10.1371/journal.pone.0043370

    Figure Lengend Snippet: Multiplex PCR for vacA subtypes and cagA gene for PG218. M, 100 bp marker; lanes 1–10, single colonies isolated from PG218 and “P” denotes pooled DNA sample; C, Positive control (26695); N, Negative control ( E. coli DNA). All the colonies isolated from this individual were cagA + and carried vacA s1 allele. Evidence of mixed infection was detected in the vacA middle region only. Lanes 1–3 and 10 yielded amplicon specific for m1, lanes 5–9 produced amplicon specific for m2 while in lane 4, no amplicon was obtained for vacA mid-region using this specific primer set.

    Article Snippet: Characterization of Strains by Multiplex PCR Multiplex PCR for the characterization of vacA s1, vacA s2, vacA m1, vacA m2 and cagA was carried out in 25 µl volume containing 2.5 pmol of primers VAG-F and VAG-R, 25 pmol of primers VA1-F and VA1-R, 10 pmol of primers cag5c-F and cag3c-R, 0.25 mM of each deoxynucleoside triphosphate (Takara), 0.9 U of Taq DNA polymerase (Genei, Bangalore, India), and 1.5 mM of MgCl2 in standard PCR buffer (Takara).

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Marker, Isolation, Positive Control, Negative Control, Infection, Amplification, Produced

    Analysis of vacA alleles by PCR and also the RFLP analysis of ∼ 3 kb fragment of vacA . M, marker; lanes 1 to 10; single colonies isolated from PG142; C, Positive control (26695); N, Negative control ( E. coli DNA). (A) Amplification of vacA s1 and m1 alleles, Lane M, 100 bp marker; all the colonies were positive for vacA s1m1. (B) Amplification of the vacA region with primer vas1F and vas GR. Lane M; 1 kb marker (Gibco BRL), Lanes 1, 3, 6, 8 and 10 failed to amplify. (C) Restriction fragment length polymorphism (RFLP) analysis of ∼3 kb fragment of vacA region using HaeIII restriction enzyme depicted microdiversity among the isolates as lane 5 showed a different digestion pattern than the rest of the colonies. Lane M, 100 bp marker.

    Journal: PLoS ONE

    Article Title: Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India

    doi: 10.1371/journal.pone.0043370

    Figure Lengend Snippet: Analysis of vacA alleles by PCR and also the RFLP analysis of ∼ 3 kb fragment of vacA . M, marker; lanes 1 to 10; single colonies isolated from PG142; C, Positive control (26695); N, Negative control ( E. coli DNA). (A) Amplification of vacA s1 and m1 alleles, Lane M, 100 bp marker; all the colonies were positive for vacA s1m1. (B) Amplification of the vacA region with primer vas1F and vas GR. Lane M; 1 kb marker (Gibco BRL), Lanes 1, 3, 6, 8 and 10 failed to amplify. (C) Restriction fragment length polymorphism (RFLP) analysis of ∼3 kb fragment of vacA region using HaeIII restriction enzyme depicted microdiversity among the isolates as lane 5 showed a different digestion pattern than the rest of the colonies. Lane M, 100 bp marker.

    Article Snippet: Characterization of Strains by Multiplex PCR Multiplex PCR for the characterization of vacA s1, vacA s2, vacA m1, vacA m2 and cagA was carried out in 25 µl volume containing 2.5 pmol of primers VAG-F and VAG-R, 25 pmol of primers VA1-F and VA1-R, 10 pmol of primers cag5c-F and cag3c-R, 0.25 mM of each deoxynucleoside triphosphate (Takara), 0.9 U of Taq DNA polymerase (Genei, Bangalore, India), and 1.5 mM of MgCl2 in standard PCR buffer (Takara).

    Techniques: Polymerase Chain Reaction, Marker, Isolation, Positive Control, Negative Control, Amplification

    Combination of genotype and RAPD analysis for PG157 indicated multiple infections and microdiversity in a single host. M, 100 bp marker; lanes 1–10, single colonies isolated from PG157; P, pooled DNA. (a) RAPD patterns using primer 1281 showed two distinct patterns (lanes 1–8 and lanes 9–10) (b) RAPD patterns using primer 1283 also yielded two distinct patterns (lanes 1–8 and lanes 9–10) (c) Multiplex PCR for vacA alleles and cagA showed existence of s1m1 cagA + strains in lanes 1–8 and s2m2 cagA − strains in lanes 9–10. (d) Variant cagA subtypes detected on the basis of PCR for 3′ end of cagA using primers CAG1 and CAG2. This PCR assay showed existence of type A strains in lanes 4, 6–8 and existence of type B/D strains in lanes 1–3 and 5. Lanes 9–10, which were detected as cagA − , did not produce any amplicon and the pooled sample yielded amplicons for type A and type B/D strains.

    Journal: PLoS ONE

    Article Title: Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India

    doi: 10.1371/journal.pone.0043370

    Figure Lengend Snippet: Combination of genotype and RAPD analysis for PG157 indicated multiple infections and microdiversity in a single host. M, 100 bp marker; lanes 1–10, single colonies isolated from PG157; P, pooled DNA. (a) RAPD patterns using primer 1281 showed two distinct patterns (lanes 1–8 and lanes 9–10) (b) RAPD patterns using primer 1283 also yielded two distinct patterns (lanes 1–8 and lanes 9–10) (c) Multiplex PCR for vacA alleles and cagA showed existence of s1m1 cagA + strains in lanes 1–8 and s2m2 cagA − strains in lanes 9–10. (d) Variant cagA subtypes detected on the basis of PCR for 3′ end of cagA using primers CAG1 and CAG2. This PCR assay showed existence of type A strains in lanes 4, 6–8 and existence of type B/D strains in lanes 1–3 and 5. Lanes 9–10, which were detected as cagA − , did not produce any amplicon and the pooled sample yielded amplicons for type A and type B/D strains.

    Article Snippet: Characterization of Strains by Multiplex PCR Multiplex PCR for the characterization of vacA s1, vacA s2, vacA m1, vacA m2 and cagA was carried out in 25 µl volume containing 2.5 pmol of primers VAG-F and VAG-R, 25 pmol of primers VA1-F and VA1-R, 10 pmol of primers cag5c-F and cag3c-R, 0.25 mM of each deoxynucleoside triphosphate (Takara), 0.9 U of Taq DNA polymerase (Genei, Bangalore, India), and 1.5 mM of MgCl2 in standard PCR buffer (Takara).

    Techniques: Marker, Isolation, Multiplex Assay, Polymerase Chain Reaction, Variant Assay, Amplification

    Multiplex PCR for vacA subtypes, cagA and cag PAI empty site for the absence of cag PAI. M, 100 bp marker (New England Biolabs); lanes 1–10, single colonies isolated from PG207; C, 26695 for the first set (a) and AM1 ( cag PAI negative strain) for the second set (b); N, Negative control ( E. coli DNA). (a) Multiplex PCR showed this particular patient was infected by at least three different strains. Lanes 1–6 and lanes 9–10 showed existence of s2m2 cagA − strains, lane 7 showed existence of s1m1 cagA − strain and lane 8 showed existence of s1m1 cagA + strain. (b) All the single colonies, which failed to give amplicon for cagA gene, yielded ∼550 bp product for cag PAI empty site. The colony (Lane 8) that produced amplicon for cagA did not show any amplicon with primers for cag PAI empty site.

    Journal: PLoS ONE

    Article Title: Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India

    doi: 10.1371/journal.pone.0043370

    Figure Lengend Snippet: Multiplex PCR for vacA subtypes, cagA and cag PAI empty site for the absence of cag PAI. M, 100 bp marker (New England Biolabs); lanes 1–10, single colonies isolated from PG207; C, 26695 for the first set (a) and AM1 ( cag PAI negative strain) for the second set (b); N, Negative control ( E. coli DNA). (a) Multiplex PCR showed this particular patient was infected by at least three different strains. Lanes 1–6 and lanes 9–10 showed existence of s2m2 cagA − strains, lane 7 showed existence of s1m1 cagA − strain and lane 8 showed existence of s1m1 cagA + strain. (b) All the single colonies, which failed to give amplicon for cagA gene, yielded ∼550 bp product for cag PAI empty site. The colony (Lane 8) that produced amplicon for cagA did not show any amplicon with primers for cag PAI empty site.

    Article Snippet: Characterization of Strains by Multiplex PCR Multiplex PCR for the characterization of vacA s1, vacA s2, vacA m1, vacA m2 and cagA was carried out in 25 µl volume containing 2.5 pmol of primers VAG-F and VAG-R, 25 pmol of primers VA1-F and VA1-R, 10 pmol of primers cag5c-F and cag3c-R, 0.25 mM of each deoxynucleoside triphosphate (Takara), 0.9 U of Taq DNA polymerase (Genei, Bangalore, India), and 1.5 mM of MgCl2 in standard PCR buffer (Takara).

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Marker, Isolation, Negative Control, Infection, Amplification, Produced

    Variant cagA subtypes detected on the basis of PCR with primers CAG1 and CAG2 amplifying the 3′ end of the gene. M, 100 bp marker; lane 1–10, single colonies isolated from individual patient and “P” denotes pooled DNA sample; C, positive control for type A cagA (cagA types were named according to the types described by Yamaoka et al. , (1998); N, Negative control ( E. coli DNA). (a) Mixed H. pylori populations were detected by obtaining amplicons for type A and type C in PG218. (b) For PG93, mixed H. pylori populations were detected by obtaining amplicons for type A and a shorter amplicon of ∼500 bp, which could not be typed by the methodology developed by Yamaoka et al. , (1998). (c) For PG144, mixed H. p ylori populations were detected by obtaining amplicons for type A and type B/D.

    Journal: PLoS ONE

    Article Title: Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India

    doi: 10.1371/journal.pone.0043370

    Figure Lengend Snippet: Variant cagA subtypes detected on the basis of PCR with primers CAG1 and CAG2 amplifying the 3′ end of the gene. M, 100 bp marker; lane 1–10, single colonies isolated from individual patient and “P” denotes pooled DNA sample; C, positive control for type A cagA (cagA types were named according to the types described by Yamaoka et al. , (1998); N, Negative control ( E. coli DNA). (a) Mixed H. pylori populations were detected by obtaining amplicons for type A and type C in PG218. (b) For PG93, mixed H. pylori populations were detected by obtaining amplicons for type A and a shorter amplicon of ∼500 bp, which could not be typed by the methodology developed by Yamaoka et al. , (1998). (c) For PG144, mixed H. p ylori populations were detected by obtaining amplicons for type A and type B/D.

    Article Snippet: Characterization of Strains by Multiplex PCR Multiplex PCR for the characterization of vacA s1, vacA s2, vacA m1, vacA m2 and cagA was carried out in 25 µl volume containing 2.5 pmol of primers VAG-F and VAG-R, 25 pmol of primers VA1-F and VA1-R, 10 pmol of primers cag5c-F and cag3c-R, 0.25 mM of each deoxynucleoside triphosphate (Takara), 0.9 U of Taq DNA polymerase (Genei, Bangalore, India), and 1.5 mM of MgCl2 in standard PCR buffer (Takara).

    Techniques: Variant Assay, Polymerase Chain Reaction, Marker, Isolation, Positive Control, Negative Control, Amplification

    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated PCR products size of the reference strain was confirmed by DNA sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.

    Journal: Osong Public Health and Research Perspectives

    Article Title: Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients

    doi: 10.1016/j.phrp.2013.09.005

    Figure Lengend Snippet: Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated PCR products size of the reference strain was confirmed by DNA sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.

    Article Snippet: The DNA amplification was performed using 15 μL of solution containing 10 ng of genomic DNA, 1 U of i-Max II DNA polymerase (iNtRON Biotechnology, Gyeonggi-do, Korea), 2.5 mM deoxyribonucleotide triphosphate mixture, 10× polymerase chain reaction (PCR) buffer, 10 pmol of each primer (Bioneer, Daejeon, Korea), 5× betaine (Sigma Aldrich, St. Louis, MO, USA), and distilled sterilized water.

    Techniques: Amplification, Polymerase Chain Reaction, DNA Sequencing

    Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, 10× buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the PCR.

    Journal: Journal of Clinical Microbiology

    Article Title: Contaminations Occurring in Fungal PCR Assays

    doi:

    Figure Lengend Snippet: Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, 10× buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the PCR.

    Article Snippet: Detailed and repeated serial analysis of all components of the PCR mixture (nucleotides, primers, water, magnesium chloride, 10× buffer, and Taq polymerase) showed a positive specific band only in the 10× PCR buffer supplied by Boehringer Mannheim.

    Techniques: Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, Amplification, Marker, Labeling, Positive Control, Negative Control, Polymerase Chain Reaction