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  • 99
    Thermo Fisher polymerase chain reaction pcr analysis
    Polymerase Chain Reaction Pcr Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polymerase chain reaction pcr analysis
    Polymerase Chain Reaction Pcr Analysis, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr analysis
    <t>PCR</t> analysis of the Epstein–Barr virus (EBV) gene from formalin-fixed and paraffin-embedded tissue. EBNA-2 (186 bp) was detected in each specimens (extra-osseous and intra-osseous area), and GAPDH and EBNA-2 bands showed the same levels
    Polymerase Chain Reaction Pcr Analysis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad pcr qpcr analysis
    <t>PCR</t> analysis of the Epstein–Barr virus (EBV) gene from formalin-fixed and paraffin-embedded tissue. EBNA-2 (186 bp) was detected in each specimens (extra-osseous and intra-osseous area), and GAPDH and EBNA-2 bands showed the same levels
    Pcr Qpcr Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche pcr analysis
    Histological features of the salivary gland in klotho−/− mice. (a) <t>PCR</t> <t>genotyping</t> of klotho‐deficient mice. Genomic DNA from mouse tails was used to amplify the fragments derived from the wild‐type and mutant alleles using two specific primers. (b) Staining with hematoxylin and eosin (×12.5 and ×100 magnification). Photomicrographs of the salivary glands in klotho wild‐type (kl+/+), hetero (kl±), and klotho‐deficient mice (kl−/−). SM, submandibular gland; SL, sublingual gland; P, parotid gland
    Pcr Analysis, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 1200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction qpcr analysis
    Histological features of the salivary gland in klotho−/− mice. (a) <t>PCR</t> <t>genotyping</t> of klotho‐deficient mice. Genomic DNA from mouse tails was used to amplify the fragments derived from the wild‐type and mutant alleles using two specific primers. (b) Staining with hematoxylin and eosin (×12.5 and ×100 magnification). Photomicrographs of the salivary glands in klotho wild‐type (kl+/+), hetero (kl±), and klotho‐deficient mice (kl−/−). SM, submandibular gland; SL, sublingual gland; P, parotid gland
    Polymerase Chain Reaction Qpcr Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare polymerase chain reaction pcr analysis
    Histological features of the salivary gland in klotho−/− mice. (a) <t>PCR</t> <t>genotyping</t> of klotho‐deficient mice. Genomic DNA from mouse tails was used to amplify the fragments derived from the wild‐type and mutant alleles using two specific primers. (b) Staining with hematoxylin and eosin (×12.5 and ×100 magnification). Photomicrographs of the salivary glands in klotho wild‐type (kl+/+), hetero (kl±), and klotho‐deficient mice (kl−/−). SM, submandibular gland; SL, sublingual gland; P, parotid gland
    Polymerase Chain Reaction Pcr Analysis, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Jackson Laboratory polymerase chain reaction pcr analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Polymerase Chain Reaction Pcr Analysis, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG pcr analysis pcr
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Pcr Analysis Pcr, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polymerase chain reaction pcr analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Polymerase Chain Reaction Pcr Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EntroGen polymerase chain reaction pcr analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Polymerase Chain Reaction Pcr Analysis, supplied by EntroGen, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PromoCell polymerase chain reaction pcr analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Polymerase Chain Reaction Pcr Analysis, supplied by PromoCell, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co polymerase chain reaction pcr analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Polymerase Chain Reaction Pcr Analysis, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Toyobo polymerase chain reaction pcr analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Polymerase Chain Reaction Pcr Analysis, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CellSearch polymerase chain reaction pcr analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Polymerase Chain Reaction Pcr Analysis, supplied by CellSearch, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meridian Life Science polymerase chain reaction pcr analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Polymerase Chain Reaction Pcr Analysis, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioMed Diagnostics Inc polymerase chain reaction analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Polymerase Chain Reaction Analysis, supplied by BioMed Diagnostics Inc, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA polymerase chain reaction analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Polymerase Chain Reaction Analysis, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene polymerase chain reaction pcr analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Polymerase Chain Reaction Pcr Analysis, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa transcription polymerase chain reaction rt pcr analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Transcription Polymerase Chain Reaction Rt Pcr Analysis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche allele specific polymerase chain reaction pcr analysis
    Cre-recombinase mediated deletion of <t>Pten</t> in SSCs. a <t>PCR</t> analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.
    Allele Specific Polymerase Chain Reaction Pcr Analysis, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    PCR analysis of the Epstein–Barr virus (EBV) gene from formalin-fixed and paraffin-embedded tissue. EBNA-2 (186 bp) was detected in each specimens (extra-osseous and intra-osseous area), and GAPDH and EBNA-2 bands showed the same levels

    Journal: Head and Neck Pathology

    Article Title: A Case of Age-Related Epstein-Barr Virus (EBV)-Associated B Cell Lymphoproliferative Disorder, so-called Polymorphous Subtype, of the Mandible, with a Review of the Literature

    doi: 10.1007/s12105-012-0392-1

    Figure Lengend Snippet: PCR analysis of the Epstein–Barr virus (EBV) gene from formalin-fixed and paraffin-embedded tissue. EBNA-2 (186 bp) was detected in each specimens (extra-osseous and intra-osseous area), and GAPDH and EBNA-2 bands showed the same levels

    Article Snippet: To demonstrate the presence of the EBV gene (EBNA-2) in surgical specimens, formalin-fixed, paraffin-embedded materials were subjected to polymerase chain reaction (PCR) analysis using a TaKaRa DEXPAT™ kit (TaKaRa, Japan) in accordance with the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction

    Histological features of the salivary gland in klotho−/− mice. (a) PCR genotyping of klotho‐deficient mice. Genomic DNA from mouse tails was used to amplify the fragments derived from the wild‐type and mutant alleles using two specific primers. (b) Staining with hematoxylin and eosin (×12.5 and ×100 magnification). Photomicrographs of the salivary glands in klotho wild‐type (kl+/+), hetero (kl±), and klotho‐deficient mice (kl−/−). SM, submandibular gland; SL, sublingual gland; P, parotid gland

    Journal: Journal of Cellular Physiology

    Article Title: Global analysis of gene expression profiles in the submandibular salivary gland of klotho knockout mice. Global analysis of gene expression profiles in the submandibular salivary gland of klotho knockout mice

    doi: 10.1002/jcp.26172

    Figure Lengend Snippet: Histological features of the salivary gland in klotho−/− mice. (a) PCR genotyping of klotho‐deficient mice. Genomic DNA from mouse tails was used to amplify the fragments derived from the wild‐type and mutant alleles using two specific primers. (b) Staining with hematoxylin and eosin (×12.5 and ×100 magnification). Photomicrographs of the salivary glands in klotho wild‐type (kl+/+), hetero (kl±), and klotho‐deficient mice (kl−/−). SM, submandibular gland; SL, sublingual gland; P, parotid gland

    Article Snippet: For genotyping PCR analysis, 1–2 mm sections of tail were dissolved in 0.1 ml of 50 mM Tris (pH 8.0), 100 mM EDTA, 0.5% SDS, and 0.5 mg/ml proteinase K (Roche) solution at 55°C for at least 1 hr with vigorous shaking.

    Techniques: Mouse Assay, Polymerase Chain Reaction, Derivative Assay, Mutagenesis, Staining

    Expression of dystrophin mRNA in the FTCVs during myogenic induction. a RT-PCR analysis. Time course of dystrophin and ribosomal RNA 18S mRNA expression at the indicated day after treatment with 5-azacytidine ( N negative control lanes without reverse transcriptase, P RNAs from human skeletal muscle as positive control). b qPCR analysis. Time course of dystrophin mRNA expression at the indicated day after treatment with 5-azacytidine. The value obtained before treatment with 5-azacytidine was set to 1. Each value ( n =3) represents the mean ± SD

    Journal: Cell and Tissue Research

    Article Title: Human first-trimester chorionic villi have a myogenic potential

    doi: 10.1007/s00441-012-1340-9

    Figure Lengend Snippet: Expression of dystrophin mRNA in the FTCVs during myogenic induction. a RT-PCR analysis. Time course of dystrophin and ribosomal RNA 18S mRNA expression at the indicated day after treatment with 5-azacytidine ( N negative control lanes without reverse transcriptase, P RNAs from human skeletal muscle as positive control). b qPCR analysis. Time course of dystrophin mRNA expression at the indicated day after treatment with 5-azacytidine. The value obtained before treatment with 5-azacytidine was set to 1. Each value ( n =3) represents the mean ± SD

    Article Snippet: For quantitative real-time reverse transcription (RT) with polymerase chain reaction (PCR) analysis (qPCR), PCR was performed by using a Fast Start Universal SYBR Green Master (ROX) kit (Roche Diagnostics, Mannheim, Germany) and the Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, Foster City, Calif., USA).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Negative Control, Positive Control, Real-time Polymerase Chain Reaction

    Phase contrast microscopic images of undifferentiated first-trimester chorionic-villi-derived cells (FTCVs;  a ) and third-trimester placental chorionic-villi-derived cells (TTCVs;  b ).  Bar  50 μm.  c  Quantitative real-time reverse transcription with polymerase chain reaction (qPCR) analysis for  Nanog ,  Sox2  and  Oct4  mRNA expression in FTCVs at the 9th (FTCV-9th), 10th (FTCV-10th) and 11th (FTCV-11th) weeks of gestation, TTCVs, normal human dermal fibroblasts (HDFs) and a pluripotent human embryonic carcinoma cell line (NTERA-2). The value for NTERA-2 was set to 1 in each experiment. Each value ( n =4) represents the mean ± SD

    Journal: Cell and Tissue Research

    Article Title: Human first-trimester chorionic villi have a myogenic potential

    doi: 10.1007/s00441-012-1340-9

    Figure Lengend Snippet: Phase contrast microscopic images of undifferentiated first-trimester chorionic-villi-derived cells (FTCVs; a ) and third-trimester placental chorionic-villi-derived cells (TTCVs; b ). Bar 50 μm. c Quantitative real-time reverse transcription with polymerase chain reaction (qPCR) analysis for Nanog , Sox2 and Oct4 mRNA expression in FTCVs at the 9th (FTCV-9th), 10th (FTCV-10th) and 11th (FTCV-11th) weeks of gestation, TTCVs, normal human dermal fibroblasts (HDFs) and a pluripotent human embryonic carcinoma cell line (NTERA-2). The value for NTERA-2 was set to 1 in each experiment. Each value ( n =4) represents the mean ± SD

    Article Snippet: For quantitative real-time reverse transcription (RT) with polymerase chain reaction (PCR) analysis (qPCR), PCR was performed by using a Fast Start Universal SYBR Green Master (ROX) kit (Roche Diagnostics, Mannheim, Germany) and the Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, Foster City, Calif., USA).

    Techniques: Derivative Assay, Reverse Transcription Polymerase Chain Reaction, Expressing

    Cre-recombinase mediated deletion of Pten in SSCs. a PCR analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.

    Journal: Cell & Bioscience

    Article Title: PTEN signaling is required for the maintenance of spermatogonial stem cells in mouse, by regulating the expressions of PLZF and UTF1

    doi: 10.1186/s13578-015-0034-x

    Figure Lengend Snippet: Cre-recombinase mediated deletion of Pten in SSCs. a PCR analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section ( scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+ , Pten +/− , and Pten −/− mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.

    Article Snippet: Genotype of Stra8 -cre mice and Pten f/f mice were determined by PCR analysis using the primers and procedures provided by the Jackson Laboratory or by a previous research [ ].

    Techniques: Polymerase Chain Reaction, Knock-Out, Mouse Assay, Positive Control, Marker, Immunostaining, Staining, Western Blot, Expressing, Isolation, Flow Cytometry, Cytometry