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    Thermo Fisher yfiler plus pcr amplification kit thermo fisher scientific
    Yfiler Plus Pcr Amplification Kit Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yfiler plus pcr amplification kit thermo fisher scientific/product/Thermo Fisher
    Average 96 stars, based on 12 article reviews
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    yfiler plus pcr amplification kit thermo fisher scientific - by Bioz Stars, 2020-09
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    99
    Thermo Fisher mmp9
    The MDA-MB-231 cells were transfected with respective siRNAs and allowed to adhere on the Matrigel coated wells for 2 hrs. ( A ) Adhered cells were stained with DAPI and photomicrographs were taken under fluorescence microscope using UV filter ( B ) The number of the adhered cells were counted. Control indicates the cells were not transfected with either of the uPA and <t>MMP9</t> siRNA. (Data are representative of three independently performed experiments and are expressed as mean ± SD).
    Mmp9, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 315 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmp9/product/Thermo Fisher
    Average 99 stars, based on 315 article reviews
    Price from $9.99 to $1999.99
    mmp9 - by Bioz Stars, 2020-09
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    Image Search Results


    The MDA-MB-231 cells were transfected with respective siRNAs and allowed to adhere on the Matrigel coated wells for 2 hrs. ( A ) Adhered cells were stained with DAPI and photomicrographs were taken under fluorescence microscope using UV filter ( B ) The number of the adhered cells were counted. Control indicates the cells were not transfected with either of the uPA and MMP9 siRNA. (Data are representative of three independently performed experiments and are expressed as mean ± SD).

    Journal: Scientific Reports

    Article Title: Simultaneous knockdown of uPA and MMP9 can reduce breast cancer progression by increasing cell-cell adhesion and modulating EMT genes

    doi: 10.1038/srep21903

    Figure Lengend Snippet: The MDA-MB-231 cells were transfected with respective siRNAs and allowed to adhere on the Matrigel coated wells for 2 hrs. ( A ) Adhered cells were stained with DAPI and photomicrographs were taken under fluorescence microscope using UV filter ( B ) The number of the adhered cells were counted. Control indicates the cells were not transfected with either of the uPA and MMP9 siRNA. (Data are representative of three independently performed experiments and are expressed as mean ± SD).

    Article Snippet: siRNA transfection Predesigned siRNA against uPA and MMP9 were procured from Ambion by life technologies, The sequences of siRNA against uPA was: 5′-GCUUAACUCCAACACGCAAtt-3′ (S) and 5′-UUGCGUGUUGGAGUUAAGCct-3′ (AS) and while siRNA against MMP9 was 5′-GACCU GGGCAGAUUCCAAAtt-3′(S) and 5′-UUUGGAAUCUGCCCAGGUCtg-3′ (AS).

    Techniques: Multiple Displacement Amplification, Transfection, Staining, Fluorescence, Microscopy

    Cells were allowed to migrate in the transwell chamber under chemotactic condition and the migrated cells were stained with DAPI and counted under fluorescence microscope. ( A ) Bargraph showing comparative migratory ability of the MDA-MB-231, MCF-7, and T47D cells ( B ) MDA-MB-231 cells were transfected with uPA and MMP9 siRNA either singly or in combination and allowed to migrate through the membrane of the transwell unit for 18 hrs under chemotactic condition. Migrated cells were stained with DAPI. The photomicrographs were taken under fluorescence microscope using UV filter ( C ) The migrated cells were counted under fluorescence microscope ( D ) Cell after transfection with respective siRNAs were allowed to invade through the matrigel coated membrane of the transwell unit for 22 hrs and stained with DAPI. Photomicrographs were taken under fluorescence microscope using UV filter ( E ) The number of the invaded cells was then counted. Control indicates the cells were not transfected with either of the uPA and MMP9 siRNA. (Data are representative of three independently performed experiments and are expressed as mean ± SD).

    Journal: Scientific Reports

    Article Title: Simultaneous knockdown of uPA and MMP9 can reduce breast cancer progression by increasing cell-cell adhesion and modulating EMT genes

    doi: 10.1038/srep21903

    Figure Lengend Snippet: Cells were allowed to migrate in the transwell chamber under chemotactic condition and the migrated cells were stained with DAPI and counted under fluorescence microscope. ( A ) Bargraph showing comparative migratory ability of the MDA-MB-231, MCF-7, and T47D cells ( B ) MDA-MB-231 cells were transfected with uPA and MMP9 siRNA either singly or in combination and allowed to migrate through the membrane of the transwell unit for 18 hrs under chemotactic condition. Migrated cells were stained with DAPI. The photomicrographs were taken under fluorescence microscope using UV filter ( C ) The migrated cells were counted under fluorescence microscope ( D ) Cell after transfection with respective siRNAs were allowed to invade through the matrigel coated membrane of the transwell unit for 22 hrs and stained with DAPI. Photomicrographs were taken under fluorescence microscope using UV filter ( E ) The number of the invaded cells was then counted. Control indicates the cells were not transfected with either of the uPA and MMP9 siRNA. (Data are representative of three independently performed experiments and are expressed as mean ± SD).

    Article Snippet: siRNA transfection Predesigned siRNA against uPA and MMP9 were procured from Ambion by life technologies, The sequences of siRNA against uPA was: 5′-GCUUAACUCCAACACGCAAtt-3′ (S) and 5′-UUGCGUGUUGGAGUUAAGCct-3′ (AS) and while siRNA against MMP9 was 5′-GACCU GGGCAGAUUCCAAAtt-3′(S) and 5′-UUUGGAAUCUGCCCAGGUCtg-3′ (AS).

    Techniques: Staining, Fluorescence, Microscopy, Multiple Displacement Amplification, Transfection

    Expression of uPA, E –cadherin, MMP2 and MMP9 in invasive ductal breast tumor. ( A ) Fold change of protein expression of uPA and E-cadherin in terms of the β-actin expression following western blot of the individual 22 tumor samples. ( B ) Representative western blot image showing the sample group based on the uPA high and uPA low expression ( C ) Representative Zymography gel for the expression of MMP9 and MMP2 (1–4, tumors with high uPA expression; 5–8, patients tumors with low uPA expression).

    Journal: Scientific Reports

    Article Title: Simultaneous knockdown of uPA and MMP9 can reduce breast cancer progression by increasing cell-cell adhesion and modulating EMT genes

    doi: 10.1038/srep21903

    Figure Lengend Snippet: Expression of uPA, E –cadherin, MMP2 and MMP9 in invasive ductal breast tumor. ( A ) Fold change of protein expression of uPA and E-cadherin in terms of the β-actin expression following western blot of the individual 22 tumor samples. ( B ) Representative western blot image showing the sample group based on the uPA high and uPA low expression ( C ) Representative Zymography gel for the expression of MMP9 and MMP2 (1–4, tumors with high uPA expression; 5–8, patients tumors with low uPA expression).

    Article Snippet: siRNA transfection Predesigned siRNA against uPA and MMP9 were procured from Ambion by life technologies, The sequences of siRNA against uPA was: 5′-GCUUAACUCCAACACGCAAtt-3′ (S) and 5′-UUGCGUGUUGGAGUUAAGCct-3′ (AS) and while siRNA against MMP9 was 5′-GACCU GGGCAGAUUCCAAAtt-3′(S) and 5′-UUUGGAAUCUGCCCAGGUCtg-3′ (AS).

    Techniques: Expressing, Western Blot, Zymography

    Effect on the reversal of the EMT. MDA-MB-231 Cells were transfected with respective siRNAs and observed after 12 hours for ch anges in the cellular morphology under phase contrast microscope. ( A ) Control cells are showing elongated spindle shaped appearance. The elongated morphology of the cells started changing towards cobble-stone shape after transfection with respective siRNAs. The cells completely acquired epithelial structure of cobble-stone shape as indicated by the arrow. (Control indicates cells were not transfected with either of the uPA or MMP9 siRNA). After trasfection of siRNA against uPA and MMP9, transcript level ( B ) uPAR ( C ) E-cadherin ( D ) Vimentin ( E ) Snail ( F ) Oct-4 were evaluated by Real time PCR. (uPA means cells were transfected with siRNA against uPA, MMP9 means cells were transfected with siRNA against MMP9 and uPA + MMP9 means cells were transfected simultaneously with siRNA against both MMP9 and uPA). Control indicates the cells were not transfected with either of the uPA and MMP9 siRNA. (Data are representative of three independently performed experiments and are expressed as mean ± SD).

    Journal: Scientific Reports

    Article Title: Simultaneous knockdown of uPA and MMP9 can reduce breast cancer progression by increasing cell-cell adhesion and modulating EMT genes

    doi: 10.1038/srep21903

    Figure Lengend Snippet: Effect on the reversal of the EMT. MDA-MB-231 Cells were transfected with respective siRNAs and observed after 12 hours for ch anges in the cellular morphology under phase contrast microscope. ( A ) Control cells are showing elongated spindle shaped appearance. The elongated morphology of the cells started changing towards cobble-stone shape after transfection with respective siRNAs. The cells completely acquired epithelial structure of cobble-stone shape as indicated by the arrow. (Control indicates cells were not transfected with either of the uPA or MMP9 siRNA). After trasfection of siRNA against uPA and MMP9, transcript level ( B ) uPAR ( C ) E-cadherin ( D ) Vimentin ( E ) Snail ( F ) Oct-4 were evaluated by Real time PCR. (uPA means cells were transfected with siRNA against uPA, MMP9 means cells were transfected with siRNA against MMP9 and uPA + MMP9 means cells were transfected simultaneously with siRNA against both MMP9 and uPA). Control indicates the cells were not transfected with either of the uPA and MMP9 siRNA. (Data are representative of three independently performed experiments and are expressed as mean ± SD).

    Article Snippet: siRNA transfection Predesigned siRNA against uPA and MMP9 were procured from Ambion by life technologies, The sequences of siRNA against uPA was: 5′-GCUUAACUCCAACACGCAAtt-3′ (S) and 5′-UUGCGUGUUGGAGUUAAGCct-3′ (AS) and while siRNA against MMP9 was 5′-GACCU GGGCAGAUUCCAAAtt-3′(S) and 5′-UUUGGAAUCUGCCCAGGUCtg-3′ (AS).

    Techniques: Multiple Displacement Amplification, Transfection, Microscopy, Real-time Polymerase Chain Reaction

    Wound healing capacity of the MDA-MB-231 cells after transfecting with uPA and MMP9 siRNA alone or in combination. ( A ) The wounded area was photographed under phase contrast microscope at 0, 24 and 48 hours after scratching. The length of gap distance was measured at 0, 24 and 48 hours using Image J software ( B ) Bar graph representing the 48 hours post recovery gap distance after induced scratch in the cell culture dish. Control indicates the cells were not transfected with either of the uPA and MMP9 siRNA. (Data are representative of three independently performed experiments and are expressed as mean ± SD).

    Journal: Scientific Reports

    Article Title: Simultaneous knockdown of uPA and MMP9 can reduce breast cancer progression by increasing cell-cell adhesion and modulating EMT genes

    doi: 10.1038/srep21903

    Figure Lengend Snippet: Wound healing capacity of the MDA-MB-231 cells after transfecting with uPA and MMP9 siRNA alone or in combination. ( A ) The wounded area was photographed under phase contrast microscope at 0, 24 and 48 hours after scratching. The length of gap distance was measured at 0, 24 and 48 hours using Image J software ( B ) Bar graph representing the 48 hours post recovery gap distance after induced scratch in the cell culture dish. Control indicates the cells were not transfected with either of the uPA and MMP9 siRNA. (Data are representative of three independently performed experiments and are expressed as mean ± SD).

    Article Snippet: siRNA transfection Predesigned siRNA against uPA and MMP9 were procured from Ambion by life technologies, The sequences of siRNA against uPA was: 5′-GCUUAACUCCAACACGCAAtt-3′ (S) and 5′-UUGCGUGUUGGAGUUAAGCct-3′ (AS) and while siRNA against MMP9 was 5′-GACCU GGGCAGAUUCCAAAtt-3′(S) and 5′-UUUGGAAUCUGCCCAGGUCtg-3′ (AS).

    Techniques: Multiple Displacement Amplification, Microscopy, Software, Cell Culture, Transfection

    Schematic diagram showing the model of simultaneous knockdown of uPA and MMP9 in breast tumor. The tumor micro-environment is represented by the presence heterogeneous cell populations – mainly epithelial cells with mesenchymal nature along with other cell types. Aggressive tumor with invasive cells may be dislodged by the reduce expression of E-cadherin. Thus, after simultaneous knockdown of uPA and MMP9, higher expression of E-cadherin and lowering of mesenchymal and stem cell marker may reduce the cellular invasion and tumor aggressiveness.

    Journal: Scientific Reports

    Article Title: Simultaneous knockdown of uPA and MMP9 can reduce breast cancer progression by increasing cell-cell adhesion and modulating EMT genes

    doi: 10.1038/srep21903

    Figure Lengend Snippet: Schematic diagram showing the model of simultaneous knockdown of uPA and MMP9 in breast tumor. The tumor micro-environment is represented by the presence heterogeneous cell populations – mainly epithelial cells with mesenchymal nature along with other cell types. Aggressive tumor with invasive cells may be dislodged by the reduce expression of E-cadherin. Thus, after simultaneous knockdown of uPA and MMP9, higher expression of E-cadherin and lowering of mesenchymal and stem cell marker may reduce the cellular invasion and tumor aggressiveness.

    Article Snippet: siRNA transfection Predesigned siRNA against uPA and MMP9 were procured from Ambion by life technologies, The sequences of siRNA against uPA was: 5′-GCUUAACUCCAACACGCAAtt-3′ (S) and 5′-UUGCGUGUUGGAGUUAAGCct-3′ (AS) and while siRNA against MMP9 was 5′-GACCU GGGCAGAUUCCAAAtt-3′(S) and 5′-UUUGGAAUCUGCCCAGGUCtg-3′ (AS).

    Techniques: Expressing, Marker