pcr amplification Search Results


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  • 99
    New England Biolabs polymerase chain reaction pcr amplification
    Polymerase Chain Reaction Pcr Amplification, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore pcr amplification
    Vectors and cloning strategy . ( A ) pAZ677 Cm R . ( B ) Construction of pENTR/Zeo by BP recombination with pDONR™/Zeo. The resulting vector has attL1, attL2 sites and two SfiI sites bordering the Cm R fragment. ( C ) The E. coli <t>ORFs</t> of pCA24N were then transferred by SfiI digestion, gel-fractionated and ligated into SfiI-digested pENTR/Zeo vector. The positive clones are selected for Zeocin resistance and Chloramphenicol sensitivity, and are validated by <t>PCR</t> and DNA sequencing (see methods).
    Pcr Amplification, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    4Gene polymerase chain reaction polymerase chain reaction pcr amplification
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Polymerase Chain Reaction Polymerase Chain Reaction Pcr Amplification, supplied by 4Gene, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction pcr amplification
    High levels of biosynthesis and secretion of IgM require <t>XBP-1.</t> (A) 10 6 live cells, as determined by trypan blue exclusion, were pulse-labeled with [ 35 S]methionine for 30 min. Cells were lysed in 1% SDS, and lysate was diluted to 0.07% SDS with NP-40 lysis mix followed by immunoprecipitation with anti-μ antibodies and analysis by SDS-PAGE (10%). (B) Autoradiograms were quantified by phosphoimager. Empty bars, WT B cells; black bars, XBP1 −/− B cells. (C) Cells stimulated for 3 d with LPS or CpG were pulse-labeled with [ 35 S]methionine for 30 min and chased for up to 4 h. Cells were lysed in 1% SDS; lysate was diluted to 0.07% SDS with NP-40 lysis mix followed by immunoprecipitation with anti-μ antibodies. At each time point, IgM also was recovered from the media by immunoprecipitated with anti-μ antibodies. Immunoprecipitates were analyzed by SDS-PAGE (10%). Each lane represents material from 10 6 live cells. (D) Autoradiograms were quantified by phosphoimager. Secreted μ chains were expressed as percentage from μ chains recovered after 4-h chase. (E) RNA was extracted from day 3 LPS-treated WT or XBP-1 −/− B cells and analyzed by Northern blotting. Quantification indicates a 2.6-fold reduction in μ mRNA levels in XBP-1 −/− cells. (F) Semi-quantitative <t>RT-PCR</t> analysis of cDNA prepared from RNA of day 3 LPS-treated WT or XBP-1 −/− B cells. Threefold dilution series of the cDNA were used as input material for the PCR with primers specific for μ chains or GAPDH as a reference. The analysis indicates a less than threefold reduction in μ mRNA levels in XBP-1 −/− cells.
    Polymerase Chain Reaction Pcr Amplification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 12019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Brinkmann Instruments polymerase chain reaction pcr amplification
    High levels of biosynthesis and secretion of IgM require <t>XBP-1.</t> (A) 10 6 live cells, as determined by trypan blue exclusion, were pulse-labeled with [ 35 S]methionine for 30 min. Cells were lysed in 1% SDS, and lysate was diluted to 0.07% SDS with NP-40 lysis mix followed by immunoprecipitation with anti-μ antibodies and analysis by SDS-PAGE (10%). (B) Autoradiograms were quantified by phosphoimager. Empty bars, WT B cells; black bars, XBP1 −/− B cells. (C) Cells stimulated for 3 d with LPS or CpG were pulse-labeled with [ 35 S]methionine for 30 min and chased for up to 4 h. Cells were lysed in 1% SDS; lysate was diluted to 0.07% SDS with NP-40 lysis mix followed by immunoprecipitation with anti-μ antibodies. At each time point, IgM also was recovered from the media by immunoprecipitated with anti-μ antibodies. Immunoprecipitates were analyzed by SDS-PAGE (10%). Each lane represents material from 10 6 live cells. (D) Autoradiograms were quantified by phosphoimager. Secreted μ chains were expressed as percentage from μ chains recovered after 4-h chase. (E) RNA was extracted from day 3 LPS-treated WT or XBP-1 −/− B cells and analyzed by Northern blotting. Quantification indicates a 2.6-fold reduction in μ mRNA levels in XBP-1 −/− cells. (F) Semi-quantitative <t>RT-PCR</t> analysis of cDNA prepared from RNA of day 3 LPS-treated WT or XBP-1 −/− B cells. Threefold dilution series of the cDNA were used as input material for the PCR with primers specific for μ chains or GAPDH as a reference. The analysis indicates a less than threefold reduction in μ mRNA levels in XBP-1 −/− cells.
    Polymerase Chain Reaction Pcr Amplification, supplied by Brinkmann Instruments, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche pcr amplification
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Pcr Amplification, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 13428 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polymerase chain reaction pcr amplification
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Pcr Amplification, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bangalore Genei polymerase chain reaction pcr amplification
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Pcr Amplification, supplied by Bangalore Genei, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction amplification polymerase chain reactions
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Amplification Polymerase Chain Reactions, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CinnaGen Co polymerase chain reaction polymerase chain reaction amplification
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Polymerase Chain Reaction Amplification, supplied by CinnaGen Co, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GeneDx Inc polymerase chain reaction amplified fragments
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
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    Millipore polymerase chain reaction pcr
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Pcr, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs polymerase chain reaction pcr
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Pcr, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 111176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biometra professional polymerase chain reaction pcr amplification instrument
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Professional Polymerase Chain Reaction Pcr Amplification Instrument, supplied by Biometra, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polymerase chain reaction pcr amplification
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Pcr Amplification, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer polymerase chain reaction pcr buffer
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Pcr Buffer, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioneer Corporation polymerase chain reaction pcr kit
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Pcr Kit, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction trizol
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Trizol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs pcr
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Pcr, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 18841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polymerase chain reaction pcr machine
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Pcr Machine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction mastermix
    <t>PCR</t> results of <t>mecA</t> gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923
    Polymerase Chain Reaction Mastermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr machine
    <t>PCR</t> amplified object fragment. The agarose gel electrophoresis revealed that the object band was in the expected position. M, DL2000 DNA marker; 1 and 2, PCR product (502 bp) <t>cDNA.</t> PCR, polymerase chain reaction.
    Polymerase Chain Reaction Pcr Machine, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Vectors and cloning strategy . ( A ) pAZ677 Cm R . ( B ) Construction of pENTR/Zeo by BP recombination with pDONR™/Zeo. The resulting vector has attL1, attL2 sites and two SfiI sites bordering the Cm R fragment. ( C ) The E. coli ORFs of pCA24N were then transferred by SfiI digestion, gel-fractionated and ligated into SfiI-digested pENTR/Zeo vector. The positive clones are selected for Zeocin resistance and Chloramphenicol sensitivity, and are validated by PCR and DNA sequencing (see methods).

    Journal: BMC Genomics

    Article Title: The Escherichia coli K-12 ORFeome: a resource for comparative molecular microbiology

    doi: 10.1186/1471-2164-11-470

    Figure Lengend Snippet: Vectors and cloning strategy . ( A ) pAZ677 Cm R . ( B ) Construction of pENTR/Zeo by BP recombination with pDONR™/Zeo. The resulting vector has attL1, attL2 sites and two SfiI sites bordering the Cm R fragment. ( C ) The E. coli ORFs of pCA24N were then transferred by SfiI digestion, gel-fractionated and ligated into SfiI-digested pENTR/Zeo vector. The positive clones are selected for Zeocin resistance and Chloramphenicol sensitivity, and are validated by PCR and DNA sequencing (see methods).

    Article Snippet: PCR amplification of the ORFs For the clones constructed by Gateway® recombinational cloning, PCR was performed in 96-well plates containing 50-μl reaction volumes consisting of 1 U KOD DNA polymerase (Novagen), dNTP mix (0.4 mM each), primary forward and reverse primers (0.3 μM each), and E. coli K-12 strain W3110 genomic DNA (200 ng).

    Techniques: Clone Assay, Plasmid Preparation, Polymerase Chain Reaction, DNA Sequencing

    Agarose gel (2%) showing PCR amplification of TLR-4 (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele

    Journal: Indian Journal of Urology : IJU : Journal of the Urological Society of India

    Article Title: Asp299Gly and Thr399Ile polymorphism of TLR-4 gene in patients with prostate cancer from North India

    doi: 10.4103/0970-1591.109982

    Figure Lengend Snippet: Agarose gel (2%) showing PCR amplification of TLR-4 (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele

    Article Snippet: Polymerase chain reaction Polymerase chain reaction (PCR) amplification of TLR-4 gene fragments was performed in Perkin Elmer thermocycler (USA) using gene-specific primers [ ].

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Variant Assay

    High levels of biosynthesis and secretion of IgM require XBP-1. (A) 10 6 live cells, as determined by trypan blue exclusion, were pulse-labeled with [ 35 S]methionine for 30 min. Cells were lysed in 1% SDS, and lysate was diluted to 0.07% SDS with NP-40 lysis mix followed by immunoprecipitation with anti-μ antibodies and analysis by SDS-PAGE (10%). (B) Autoradiograms were quantified by phosphoimager. Empty bars, WT B cells; black bars, XBP1 −/− B cells. (C) Cells stimulated for 3 d with LPS or CpG were pulse-labeled with [ 35 S]methionine for 30 min and chased for up to 4 h. Cells were lysed in 1% SDS; lysate was diluted to 0.07% SDS with NP-40 lysis mix followed by immunoprecipitation with anti-μ antibodies. At each time point, IgM also was recovered from the media by immunoprecipitated with anti-μ antibodies. Immunoprecipitates were analyzed by SDS-PAGE (10%). Each lane represents material from 10 6 live cells. (D) Autoradiograms were quantified by phosphoimager. Secreted μ chains were expressed as percentage from μ chains recovered after 4-h chase. (E) RNA was extracted from day 3 LPS-treated WT or XBP-1 −/− B cells and analyzed by Northern blotting. Quantification indicates a 2.6-fold reduction in μ mRNA levels in XBP-1 −/− cells. (F) Semi-quantitative RT-PCR analysis of cDNA prepared from RNA of day 3 LPS-treated WT or XBP-1 −/− B cells. Threefold dilution series of the cDNA were used as input material for the PCR with primers specific for μ chains or GAPDH as a reference. The analysis indicates a less than threefold reduction in μ mRNA levels in XBP-1 −/− cells.

    Journal: The Journal of Experimental Medicine

    Article Title: XBP-1 specifically promotes IgM synthesis and secretion, but is dispensable for degradation of glycoproteins in primary B cells

    doi: 10.1084/jem.20050575

    Figure Lengend Snippet: High levels of biosynthesis and secretion of IgM require XBP-1. (A) 10 6 live cells, as determined by trypan blue exclusion, were pulse-labeled with [ 35 S]methionine for 30 min. Cells were lysed in 1% SDS, and lysate was diluted to 0.07% SDS with NP-40 lysis mix followed by immunoprecipitation with anti-μ antibodies and analysis by SDS-PAGE (10%). (B) Autoradiograms were quantified by phosphoimager. Empty bars, WT B cells; black bars, XBP1 −/− B cells. (C) Cells stimulated for 3 d with LPS or CpG were pulse-labeled with [ 35 S]methionine for 30 min and chased for up to 4 h. Cells were lysed in 1% SDS; lysate was diluted to 0.07% SDS with NP-40 lysis mix followed by immunoprecipitation with anti-μ antibodies. At each time point, IgM also was recovered from the media by immunoprecipitated with anti-μ antibodies. Immunoprecipitates were analyzed by SDS-PAGE (10%). Each lane represents material from 10 6 live cells. (D) Autoradiograms were quantified by phosphoimager. Secreted μ chains were expressed as percentage from μ chains recovered after 4-h chase. (E) RNA was extracted from day 3 LPS-treated WT or XBP-1 −/− B cells and analyzed by Northern blotting. Quantification indicates a 2.6-fold reduction in μ mRNA levels in XBP-1 −/− cells. (F) Semi-quantitative RT-PCR analysis of cDNA prepared from RNA of day 3 LPS-treated WT or XBP-1 −/− B cells. Threefold dilution series of the cDNA were used as input material for the PCR with primers specific for μ chains or GAPDH as a reference. The analysis indicates a less than threefold reduction in μ mRNA levels in XBP-1 −/− cells.

    Article Snippet: PCR primers 5′-ACACGCTTGGGAATGGACAC-3′ and 5′-CCATGGGAAGATGTTATGGG-3′, encompassing the missing sequences in XBP-1, were used for the PCR amplification with Platinum PCR Supermix (Invitrogen).

    Techniques: Labeling, Lysis, Immunoprecipitation, SDS Page, Northern Blot, Quantitative RT-PCR, Polymerase Chain Reaction

    XBP-1 controls plasmablasts' cell size. (A) WT or XBP-1 −/− B cells were purified from splenocytes by magnetic depletion with anti-CD43. Cells were plated at 10 6 cells/ml and stimulated with CpG. Flow cytometry analysis was performed every 24 h, and live cells were gated based on their forward and side scattering. Cells were replated at 10 6 cells/ml density and were analyzed the next day. Line graphs of the gated cells at the forward scatter channel (FSC) are shown in the right column. Gray, WT cells; white, XBP-1 −/− cells. The percentage of dead cells is indicated. (B) Cells were stimulated with CpG for 4 d. RNA was extracted at the indicated times, and splicing of XBP-1 mRNA was analyzed by RT-PCR. Tunicamycin treatment (1μg/ml, 4 h) of naive B cells was used as a positive control.

    Journal: The Journal of Experimental Medicine

    Article Title: XBP-1 specifically promotes IgM synthesis and secretion, but is dispensable for degradation of glycoproteins in primary B cells

    doi: 10.1084/jem.20050575

    Figure Lengend Snippet: XBP-1 controls plasmablasts' cell size. (A) WT or XBP-1 −/− B cells were purified from splenocytes by magnetic depletion with anti-CD43. Cells were plated at 10 6 cells/ml and stimulated with CpG. Flow cytometry analysis was performed every 24 h, and live cells were gated based on their forward and side scattering. Cells were replated at 10 6 cells/ml density and were analyzed the next day. Line graphs of the gated cells at the forward scatter channel (FSC) are shown in the right column. Gray, WT cells; white, XBP-1 −/− cells. The percentage of dead cells is indicated. (B) Cells were stimulated with CpG for 4 d. RNA was extracted at the indicated times, and splicing of XBP-1 mRNA was analyzed by RT-PCR. Tunicamycin treatment (1μg/ml, 4 h) of naive B cells was used as a positive control.

    Article Snippet: PCR primers 5′-ACACGCTTGGGAATGGACAC-3′ and 5′-CCATGGGAAGATGTTATGGG-3′, encompassing the missing sequences in XBP-1, were used for the PCR amplification with Platinum PCR Supermix (Invitrogen).

    Techniques: Purification, Flow Cytometry, Cytometry, Reverse Transcription Polymerase Chain Reaction, Positive Control

    PCR results of mecA gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923

    Journal: Advanced Biomedical Research

    Article Title: Detection of methicillin-resistance gene in Staphylococcus epidermidis strains isolated from patients in Al-Zahra Hospital using polymerase chain reaction and minimum inhibitory concentration methods

    doi: 10.4103/2277-9175.108008

    Figure Lengend Snippet: PCR results of mecA gene in 7 isolates staphylococcus epidermidis. Column 10: Size marker (50 bp). Column 8, 1-6: Isolates of Staphylococcus epidermidis containing mecA gene. Column 7: Positive control of staphylococcus aureus ATC 33591. Column 9: Negative control of staphylococcus aureus ATCC 25923

    Article Snippet: The following primers were used for PCR amplification of mecA gene:[ ] mecA-F: 5′-TGGCTATCGTGTCACAATCG-3′ mecA-R: 5′-CTGGAACTTGTTGAGCAGAG-3′ PCR was performed in a mixture of 25 μl volume containing: 2.5 μl 10 × buffer (Roche Germany, Berlin), 0.4 μl of each dNTP (200 μm), 2.5 μl (50 mm) MgCl2, 2.5 U of Taq DNA polymerase, 10 pmol of each primer, and 5 μl of template DNA.

    Techniques: Polymerase Chain Reaction, Marker, Positive Control, Negative Control

    PCR amplified object fragment. The agarose gel electrophoresis revealed that the object band was in the expected position. M, DL2000 DNA marker; 1 and 2, PCR product (502 bp) cDNA. PCR, polymerase chain reaction.

    Journal: Molecular Medicine Reports

    Article Title: Lentivirus transduced interleukin-1 receptor antagonist gene expression in murine bone marrow-derived mesenchymal stem cells in vitro

    doi: 10.3892/mmr.2015.4003

    Figure Lengend Snippet: PCR amplified object fragment. The agarose gel electrophoresis revealed that the object band was in the expected position. M, DL2000 DNA marker; 1 and 2, PCR product (502 bp) cDNA. PCR, polymerase chain reaction.

    Article Snippet: The IL-1Ra cDNA was amplified using a polymerase chain reaction (PCR) machine (Takara Bio, Inc.) and the expression vector was cloned and validated by sequencing (Invitrogen Life Technologies).

    Techniques: Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Marker