Journal: The Journal of General Virology
Article Title: Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus
Figure Lengend Snippet: Primers specific for OdAdV-1 were designed for a diagnostic PCR and a genotyping PCR. Assays were run using DNA extracted from additional diagnostic cases that were positive for OdAdV-1 or other viral agents to test for specificity. (a) The diagnostic PCR amplified a 502 bp amplicon, a 100 bp molecular weight marker, and OdAdV-1 from mule deer (lanes 1, 3), elk (lane 2), pronghorn (lanes 4, 8) and moose (lane 11). No amplification product was produced with samples positive for bovine, canine, or ovine adenovirus of undetermined type (lanes 5–7), chlamydophila from a mule deer (lane 9), or the no template control (lane 10). (b) The genotyping PCR was designed to span part of the variable region and produced amplicons of 680, 701, or 733 bp, depending on the presence or absence of deletions: lanes 1–4, California black-tailed deer (680 bp due to 53 bp deletion); lanes 5–8, pronghorn, white-tailed deer, mule deer, (701 bp due to 32 bp deletion); and lane 8–9, mule deer with the moose/elk variation (733 bp with no deletions). Traditional Sanger sequencing using forward and reverse primers confirmed the expected sequence changes.
Article Snippet: Sanger sequencing of the PCR amplicons using forward and reverse primers (GENEWIZ, South Plainfield, NJ, USA) was used to verify the amplicons.
Techniques: Diagnostic Assay, Polymerase Chain Reaction, Amplification, Molecular Weight, Marker, Produced, Sequencing