pcr 2.1 plasmid Search Results


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  • 99
    Thermo Fisher plasmid pcr 2 1 topo
    Plasmid Pcr 2 1 Topo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid pcr 2 1 topo/product/Thermo Fisher
    Average 99 stars, based on 523 article reviews
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    89
    Thermo Fisher pcr 2 1 plasmid
    PCR products from ligation mixtures using vector specific primers M13-R    M-13F.  Electrophoresis on 1.2 % agarose gel and staining with Ethidium Bromide. A: Band 1 derived from recombinants and Band 2 from relegated pCR 2.1 vector. Lane 2    3 has two types of recombinant specific products and both were used for calculation band intensity/amount. B: PCR product amounts calculated as corresponding band intensity (mean grey value) using ImageJ 1.33 software  (http://rsb.info.nih.gov/ij) .
    Pcr 2 1 Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 plasmid/product/Thermo Fisher
    Average 89 stars, based on 418 article reviews
    Price from $9.99 to $1999.99
    pcr 2 1 plasmid - by Bioz Stars, 2020-08
    89/100 stars
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    88
    Thermo Fisher topo pcr 2 1 plasmids
    Amplification of DNA fragments encoding for GD 2 -mimicking paratopes of ganglidiximab. ( A ) Visualization of coding sequences of GD 2 -mimicking variable heavy (VH; 440 bp) and light chain (VL; 420 bp) amplified by RT-PCR. RNA was isolated from hybridoma cells producing murine anti-Id ganglidiomab. PCR products were analyzed by agarose gel electrophoresis. Representative image is shown. NTC—no-template-control. M—Marker (100-bp). ( B ) PCR products were cloned into pCR ® <t>2.1-TOPO</t> ® plasmids and analyzed by restriction enzyme digest to excise DNA sequences encoding for VH and VL (product sizes 427 bp and 407 bp, respectively). Resulting DNA fragments were analyzed by agarose gel electrophoresis. Representative image is shown. M—Marker (2-log, 0.1–10.0 kbp).
    Topo Pcr 2 1 Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/topo pcr 2 1 plasmids/product/Thermo Fisher
    Average 88 stars, based on 150 article reviews
    Price from $9.99 to $1999.99
    topo pcr 2 1 plasmids - by Bioz Stars, 2020-08
    88/100 stars
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    88
    Eurofins pcr 2 1 plasmid vector
    Standard curve generated from quantification cycle numbers of a 10-fold dilution series of an amplicon product of 86 bp cloned into a pCR 2.1 plasmid vector in DNase- and RNase-free water (≤5.00 to 5.00 × 10 5  copies/reaction). qPCR was
    Pcr 2 1 Plasmid Vector, supplied by Eurofins, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 plasmid vector/product/Eurofins
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pcr 2 1 plasmid vector - by Bioz Stars, 2020-08
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    80
    Thermo Fisher ta cloning plasmid pcr 2 1
    Standard curve generated from quantification cycle numbers of a 10-fold dilution series of an amplicon product of 86 bp cloned into a pCR 2.1 plasmid vector in DNase- and RNase-free water (≤5.00 to 5.00 × 10 5  copies/reaction). qPCR was
    Ta Cloning Plasmid Pcr 2 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Stratagene plasmid vector pcr 2 1
    Standard curve generated from quantification cycle numbers of a 10-fold dilution series of an amplicon product of 86 bp cloned into a pCR 2.1 plasmid vector in DNase- and RNase-free water (≤5.00 to 5.00 × 10 5  copies/reaction). qPCR was
    Plasmid Vector Pcr 2 1, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid vector pcr 2 1/product/Stratagene
    Average 88 stars, based on 8 article reviews
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    80
    Thermo Fisher pcr 2 1 sequencing plasmids
    Standard curve generated from quantification cycle numbers of a 10-fold dilution series of an amplicon product of 86 bp cloned into a pCR 2.1 plasmid vector in DNase- and RNase-free water (≤5.00 to 5.00 × 10 5  copies/reaction). qPCR was
    Pcr 2 1 Sequencing Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    MWG-Biotech pcr2 1 plasmid
    Standard curve generated from quantification cycle numbers of a 10-fold dilution series of an amplicon product of 86 bp cloned into a pCR 2.1 plasmid vector in DNase- and RNase-free water (≤5.00 to 5.00 × 10 5  copies/reaction). qPCR was
    Pcr2 1 Plasmid, supplied by MWG-Biotech, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    PCR products from ligation mixtures using vector specific primers M13-R    M-13F.  Electrophoresis on 1.2 % agarose gel and staining with Ethidium Bromide. A: Band 1 derived from recombinants and Band 2 from relegated pCR 2.1 vector. Lane 2    3 has two types of recombinant specific products and both were used for calculation band intensity/amount. B: PCR product amounts calculated as corresponding band intensity (mean grey value) using ImageJ 1.33 software  (http://rsb.info.nih.gov/ij) .

    Journal: Biological Procedures Online

    Article Title: Analyzing ligation mixtures using a PCR based method

    doi: 10.1251/bpo108

    Figure Lengend Snippet: PCR products from ligation mixtures using vector specific primers M13-R M-13F. Electrophoresis on 1.2 % agarose gel and staining with Ethidium Bromide. A: Band 1 derived from recombinants and Band 2 from relegated pCR 2.1 vector. Lane 2 3 has two types of recombinant specific products and both were used for calculation band intensity/amount. B: PCR product amounts calculated as corresponding band intensity (mean grey value) using ImageJ 1.33 software (http://rsb.info.nih.gov/ij) .

    Article Snippet: Reagents Plasmid cloning vector: pCR 2.1 plasmid (Invitrogen, Carlsbad, CA, USA) Restriction enzymes: EcoR I, Hind III DNA ligase: T4 DNA ligase Competent cells: One Shot® Top10 E.coli chemically competent cells (Invitrogen, Carlsbad, CA, USA) Milli-Q water Plasmid specific primers: M13-forward and M13-reverse primers PCR reagents: 10X reaction buffer, dNTP mix (2.5 mM each) and Taq DNA polymerase Eppendorf/Brinkmann, Westbury, NY, USA) Agarose: SeaKem LE agarose (BioWhittaker Molecular Applications, Rockland, ME, USA) Ethidium Bromide.

    Techniques: Polymerase Chain Reaction, Ligation, Plasmid Preparation, Electrophoresis, Agarose Gel Electrophoresis, Staining, Derivative Assay, Recombinant, Software

    Strategy used for generating inserts for ligation.  A. Generating inserts for 60S-L37 and 40S-L17 using PCR from Aedes salivary gland cDNA clones for TA and cohesive end cloning. CDS-III 3’ primer used as insert specific primer. B. pCR 2.1 plasmid vector (Invitrogen, Carlsbad, CA, USA) used for TA and cohesive end cloning (Map not to scale. Complete vector sequence and map can be found at www.invitrogen.com).

    Journal: Biological Procedures Online

    Article Title: Analyzing ligation mixtures using a PCR based method

    doi: 10.1251/bpo108

    Figure Lengend Snippet: Strategy used for generating inserts for ligation. A. Generating inserts for 60S-L37 and 40S-L17 using PCR from Aedes salivary gland cDNA clones for TA and cohesive end cloning. CDS-III 3’ primer used as insert specific primer. B. pCR 2.1 plasmid vector (Invitrogen, Carlsbad, CA, USA) used for TA and cohesive end cloning (Map not to scale. Complete vector sequence and map can be found at www.invitrogen.com).

    Article Snippet: Reagents Plasmid cloning vector: pCR 2.1 plasmid (Invitrogen, Carlsbad, CA, USA) Restriction enzymes: EcoR I, Hind III DNA ligase: T4 DNA ligase Competent cells: One Shot® Top10 E.coli chemically competent cells (Invitrogen, Carlsbad, CA, USA) Milli-Q water Plasmid specific primers: M13-forward and M13-reverse primers PCR reagents: 10X reaction buffer, dNTP mix (2.5 mM each) and Taq DNA polymerase Eppendorf/Brinkmann, Westbury, NY, USA) Agarose: SeaKem LE agarose (BioWhittaker Molecular Applications, Rockland, ME, USA) Ethidium Bromide.

    Techniques: Ligation, Polymerase Chain Reaction, Clone Assay, Plasmid Preparation, Sequencing

    Western immunoblot of PsaA protein performed with the F1-1B MAb. Lanes: 1, S. pneumoniae strain R6 whole-cell antigen preparations; 2, protein standards; 3 and 4, lysates from two clones of E. coli INVαF′ transformed with the vector plasmid pCR2.1; 5 and 6, lysates from two clones of E. coli INVαF′ harboring the recombinant plasmid pCRNB2. Numbers on the left are molecular masses, in kilodaltons.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: Identification of the psaA Gene, Coding for Pneumococcal Surface Adhesin A, in Viridans Group Streptococci other than Streptococcus pneumoniae

    doi: 10.1128/CDLI.8.5.895-898.2001

    Figure Lengend Snippet: Western immunoblot of PsaA protein performed with the F1-1B MAb. Lanes: 1, S. pneumoniae strain R6 whole-cell antigen preparations; 2, protein standards; 3 and 4, lysates from two clones of E. coli INVαF′ transformed with the vector plasmid pCR2.1; 5 and 6, lysates from two clones of E. coli INVαF′ harboring the recombinant plasmid pCRNB2. Numbers on the left are molecular masses, in kilodaltons.

    Article Snippet: The pCR2.1 plasmid (Invitrogen, Carlsbad, Calif.) was used for cloning.

    Techniques: Western Blot, Clone Assay, Transformation Assay, Plasmid Preparation, Recombinant

    Amplification of DNA fragments encoding for GD 2 -mimicking paratopes of ganglidiximab. ( A ) Visualization of coding sequences of GD 2 -mimicking variable heavy (VH; 440 bp) and light chain (VL; 420 bp) amplified by RT-PCR. RNA was isolated from hybridoma cells producing murine anti-Id ganglidiomab. PCR products were analyzed by agarose gel electrophoresis. Representative image is shown. NTC—no-template-control. M—Marker (100-bp). ( B ) PCR products were cloned into pCR ® 2.1-TOPO ® plasmids and analyzed by restriction enzyme digest to excise DNA sequences encoding for VH and VL (product sizes 427 bp and 407 bp, respectively). Resulting DNA fragments were analyzed by agarose gel electrophoresis. Representative image is shown. M—Marker (2-log, 0.1–10.0 kbp).

    Journal: PLoS ONE

    Article Title: Generation and Characterization of a Human/Mouse Chimeric GD2-Mimicking Anti-Idiotype Antibody Ganglidiximab for Active Immunotherapy against Neuroblastoma

    doi: 10.1371/journal.pone.0150479

    Figure Lengend Snippet: Amplification of DNA fragments encoding for GD 2 -mimicking paratopes of ganglidiximab. ( A ) Visualization of coding sequences of GD 2 -mimicking variable heavy (VH; 440 bp) and light chain (VL; 420 bp) amplified by RT-PCR. RNA was isolated from hybridoma cells producing murine anti-Id ganglidiomab. PCR products were analyzed by agarose gel electrophoresis. Representative image is shown. NTC—no-template-control. M—Marker (100-bp). ( B ) PCR products were cloned into pCR ® 2.1-TOPO ® plasmids and analyzed by restriction enzyme digest to excise DNA sequences encoding for VH and VL (product sizes 427 bp and 407 bp, respectively). Resulting DNA fragments were analyzed by agarose gel electrophoresis. Representative image is shown. M—Marker (2-log, 0.1–10.0 kbp).

    Article Snippet: Cloning of murine ganglidiomab variable region into pCR® 2.1-TOPO® plasmid After purification, ganglidiomab VH and -VL were cloned into pCR® 2.1-TOPO® plasmids (LifeTechnologies GmbH, Darmstadt, Germany) according to the manufacturer’s guidelines.

    Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction, Isolation, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker, Clone Assay

    Standard curve generated from quantification cycle numbers of a 10-fold dilution series of an amplicon product of 86 bp cloned into a pCR 2.1 plasmid vector in DNase- and RNase-free water (≤5.00 to 5.00 × 10 5  copies/reaction). qPCR was

    Journal: Applied and Environmental Microbiology

    Article Title: Enumeration of Salmonellae in Table Eggs, Pasteurized Egg Products, and Egg-Containing Dishes by Using Quantitative Real-Time PCR

    doi: 10.1128/AEM.03360-13

    Figure Lengend Snippet: Standard curve generated from quantification cycle numbers of a 10-fold dilution series of an amplicon product of 86 bp cloned into a pCR 2.1 plasmid vector in DNase- and RNase-free water (≤5.00 to 5.00 × 10 5 copies/reaction). qPCR was

    Article Snippet: In order to establish the detection limit, an amplicon product of 86 bp cloned into a pCR 2.1 plasmid vector (Eurofins MWG Operon, Ebersberg, Germany) was used.

    Techniques: Generated, Amplification, Clone Assay, Polymerase Chain Reaction, Plasmid Preparation, Real-time Polymerase Chain Reaction