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  • 86
    4Gene polymerase chain reaction polymerase chain reaction pcr amplification
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Polymerase Chain Reaction Polymerase Chain Reaction Pcr Amplification, supplied by 4Gene, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG quantitative polymerase chain reaction polymerase chain reactions
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Quantitative Polymerase Chain Reaction Polymerase Chain Reactions, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction polymerase chain reaction pcr
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Polymerase Chain Reaction Polymerase Chain Reaction Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meridian Life Science polymerase chain reaction polymerase chain reaction pcr
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Polymerase Chain Reaction Polymerase Chain Reaction Pcr, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CinnaGen Co polymerase chain reaction polymerase chain reaction amplification
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Polymerase Chain Reaction Polymerase Chain Reaction Amplification, supplied by CinnaGen Co, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SuperArray Bioscience Corporation transcription rt polymerase chain reaction polymerase chain reaction pcr kit
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Transcription Rt Polymerase Chain Reaction Polymerase Chain Reaction Pcr Kit, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MJ Research polymerase chain reaction pcr polymerase chain reactions
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Polymerase Chain Reaction Pcr Polymerase Chain Reactions, supplied by MJ Research, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DaAn Gene epstein barr virus ebv polymerase chain reaction polymerase chain reaction pcr fluorescence detection kit
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Epstein Barr Virus Ebv Polymerase Chain Reaction Polymerase Chain Reaction Pcr Fluorescence Detection Kit, supplied by DaAn Gene, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa transcription rt polymerase chain reaction polymerase chain reaction pcr kit
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Transcription Rt Polymerase Chain Reaction Polymerase Chain Reaction Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MJ Research real time polymerase chain reaction polymerase chain reaction pcr
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Real Time Polymerase Chain Reaction Polymerase Chain Reaction Pcr, supplied by MJ Research, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Seegene virus rv polymerase chain reaction polymerase chain reaction pcr test
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Virus Rv Polymerase Chain Reaction Polymerase Chain Reaction Pcr Test, supplied by Seegene, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche quantitative real time polymerase chain reaction polymerase chain reaction pcr
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Quantitative Real Time Polymerase Chain Reaction Polymerase Chain Reaction Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche transcriptase rt polymerase chain reaction polymerase chain reaction pcr technique
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Transcriptase Rt Polymerase Chain Reaction Polymerase Chain Reaction Pcr Technique, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher transcriptase rt polymerase chain reaction polymerase chain reaction pcr kit
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Transcriptase Rt Polymerase Chain Reaction Polymerase Chain Reaction Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time rt polymerase chain reaction polymerase chain reaction pcr instruments
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Real Time Rt Polymerase Chain Reaction Polymerase Chain Reaction Pcr Instruments, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction amplification polymerase chain reactions
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Polymerase Chain Reaction Amplification Polymerase Chain Reactions, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co polymerase chain reaction pcr
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Polymerase Chain Reaction Pcr, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 95/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TransGen biotech co polymerase chain reaction pcr
    Agarose gel (2%) showing <t>PCR</t> amplification of <t>TLR-4</t> (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele
    Polymerase Chain Reaction Pcr, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction pcr
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 77775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ReaMetrix pcr polymerase chain reaction
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Pcr Polymerase Chain Reaction, supplied by ReaMetrix, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioneer Corporation polymerase chain reactions pcr primers
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reactions Pcr Primers, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Brinkmann Instruments polymerase chain reaction pcr amplification
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr Amplification, supplied by Brinkmann Instruments, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer polymerase chain reaction pcr buffer
    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by <t>qRT-PCR.</t> Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p
    Polymerase Chain Reaction Pcr Buffer, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr kit
    (A) Identification of the fat-1 gene by <t>PCR;</t> screen of fat-1 gene positive <t>C57BL6</t> mice and fat-1 gene negative C57BL6 mice. (B) Gas liquid chromatography measured the amount of n-3 PUFAs and n-6 PUFAs of the mouse tail. PUFAs, polyunsaturated fatty acids; PCR, polymerase chain reaction.
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    (A) Identification of the fat-1 gene by <t>PCR;</t> screen of fat-1 gene positive <t>C57BL6</t> mice and fat-1 gene negative C57BL6 mice. (B) Gas liquid chromatography measured the amount of n-3 PUFAs and n-6 PUFAs of the mouse tail. PUFAs, polyunsaturated fatty acids; PCR, polymerase chain reaction.
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    (A) Identification of the fat-1 gene by <t>PCR;</t> screen of fat-1 gene positive <t>C57BL6</t> mice and fat-1 gene negative C57BL6 mice. (B) Gas liquid chromatography measured the amount of n-3 PUFAs and n-6 PUFAs of the mouse tail. PUFAs, polyunsaturated fatty acids; PCR, polymerase chain reaction.
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    (A) Identification of the fat-1 gene by <t>PCR;</t> screen of fat-1 gene positive <t>C57BL6</t> mice and fat-1 gene negative C57BL6 mice. (B) Gas liquid chromatography measured the amount of n-3 PUFAs and n-6 PUFAs of the mouse tail. PUFAs, polyunsaturated fatty acids; PCR, polymerase chain reaction.
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    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
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    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
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    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
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    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time <t>RT-PCR</t> for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with <t>U6.</t> Cord blood (CB) and peripheral blood (PB)
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    Image Search Results


    Agarose gel (2%) showing PCR amplification of TLR-4 (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele

    Journal: Indian Journal of Urology : IJU : Journal of the Urological Society of India

    Article Title: Asp299Gly and Thr399Ile polymorphism of TLR-4 gene in patients with prostate cancer from North India

    doi: 10.4103/0970-1591.109982

    Figure Lengend Snippet: Agarose gel (2%) showing PCR amplification of TLR-4 (a, b) and restriction digestion of PCR products (c, d). Lanes M:Φ×BsuR1(HaeIII)[NEB]; 5-11 (c) and 1,2,6-9 (d): wild-type allele; 1,2 (c) and 3,4,10 (d) heterozygous alleles; 3,4 (c) and 11 (d): homozygous variant allele

    Article Snippet: Polymerase chain reaction Polymerase chain reaction (PCR) amplification of TLR-4 gene fragments was performed in Perkin Elmer thermocycler (USA) using gene-specific primers [ ].

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Variant Assay

    Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by qRT-PCR. Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p

    Journal: Stem Cells (Dayton, Ohio)

    Article Title: Calcium and Calcineurin-NFAT Signaling Regulate Granulocyte-Monocyte Progenitor Cell Cycle via Flt3-L

    doi: 10.1002/stem.1813

    Figure Lengend Snippet: Calcineurin-nuclear factor of activated T cells (NFAT) inhibition results in increased granulocyte–monocyte progenitors (GMP) proliferation in vitro, due to changes in the expression of cell cycle control genes in FLT-3L-differentiated progenitors. (A): Heat maps reflecting changes in the expression of genes related to cell cycle regulation, transcriptional regulation of differentiation, and hematopoiesis are shown. cKIT + -enriched cells were cultured in hematopoietic stem cell (HSC) medium stimulated with of rmFlt3-L (300 ng/ml) in the presence or absence of Cyclosporine A (CsA) (24 and 48 hours). Data represent three replicates per time point and treatment. Cells from 10 mice were pooled for each biological replicate. (B–D): Differential expression of Cdk4, Cdk6 , and Cdkn1a ( p21 ) mRNA within progenitor populations. Sorted HSCs (lin − , cKit + , Sca-1 + , CD34 + , Flt3 − ), multipotent progenitors (MPPs) (lin − , cKit + , Sca-1 + , CD34 + , Flt3 + ), common myeloid progenitors (CMPs) (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 − ), and GMPs (lin − , cKit + , Sca-1 − , CD34 + , CD16/32 + ) were cultured for 24 hours in HSC medium in the presence of Flt3-L (300 ng/ml) before the expression of main cell cycle regulatory genes was measured by qRT-PCR. Data represent at least five independent experiments, bone marrow (BM) from 15 mice pooled for each. One-way analysis of variance (ANOVA) with Turkey multiple comparisons test was used, *, p

    Article Snippet: Reverse transcription was carried out using high-capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems), or with SuperScript III First Strand Synthesis System for RT-polymerase chain reaction (PCR) (Invitrogen).

    Techniques: Inhibition, In Vitro, Expressing, Cell Culture, Mouse Assay, Quantitative RT-PCR

    (A) Identification of the fat-1 gene by PCR; screen of fat-1 gene positive C57BL6 mice and fat-1 gene negative C57BL6 mice. (B) Gas liquid chromatography measured the amount of n-3 PUFAs and n-6 PUFAs of the mouse tail. PUFAs, polyunsaturated fatty acids; PCR, polymerase chain reaction.

    Journal: Molecular Medicine Reports

    Article Title: Endogenous n-3 polyunsaturated fatty acids protect against imiquimod-induced psoriasis-like inflammation via the IL-17/IL-23 axis

    doi: 10.3892/mmr.2014.2136

    Figure Lengend Snippet: (A) Identification of the fat-1 gene by PCR; screen of fat-1 gene positive C57BL6 mice and fat-1 gene negative C57BL6 mice. (B) Gas liquid chromatography measured the amount of n-3 PUFAs and n-6 PUFAs of the mouse tail. PUFAs, polyunsaturated fatty acids; PCR, polymerase chain reaction.

    Article Snippet: Male fat-1 transgenic mice were mated with wild-type C57BL6 female mice to obtain female fat-1 positive C57BL6 mice (fat-1) and fat-1 negative C57BL6 mice (WT) identified by genotyping using a polymerase chain reaction (PCR) kit purchased from (Takara Bio, Inc.; Dalian, Liaoning, China; ).

    Techniques: Polymerase Chain Reaction, Mouse Assay, Gas Chromatography

    miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time RT-PCR for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with U6. Cord blood (CB) and peripheral blood (PB)

    Journal: Blood

    Article Title: Cell-cycle regulator E2F1 and microRNA-223 comprise an autoregulatory negative feedback loop in acute myeloid leukemia

    doi: 10.1182/blood-2009-08-240101

    Figure Lengend Snippet: miR-223 functions as a tumor suppressor in acute myeloid leukemia . (A) Quantitative real-time RT-PCR for miR-223 was carried out using bone marrow cells derived from AML patients. Values were normalized with U6. Cord blood (CB) and peripheral blood (PB)

    Article Snippet: Corresponding reverse-transcription (RT) and polymerase chain reaction (PCR) primers for miRNA-223 and U6 were obtained from Applied Biosystems.

    Techniques: Quantitative RT-PCR, Derivative Assay