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  • 99
    Thermo Fisher pcna positive cells
    Pcna Positive Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology proliferating cell nuclear antigen pcna
    Proliferating Cell Nuclear Antigen Pcna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Cell Signaling Technology Inc t proliferating cell nuclear antigen pcna
    T Proliferating Cell Nuclear Antigen Pcna, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam anti proliferating cell nuclear antigen pcna
    The function of heterochromatin protein 1γ (HP1γ) in the DNA damage response pathway. (A) Bloom syndrome protein <t>(BLM)</t> and proliferating cell nuclear antigen <t>(PCNA)</t> were associated with endogenous HP1γ proteins. BLM and PCNA were coimmunoprecipitated with anti-HP1γ antibodies using HEK293T cell lysates and immunoblotted with indicated antibodies. (B) HeLa cells were treated with 50 μM of etoposide or vehicle (dimethyl sulfoxide, DMSO) for 6 hours and immunostained with the indicated antibodies. (C) HeLa cells were transfected with pSUPER-siHP1γ and immunostained with the indicated antibodies. (D) Nonproportional Venn diagram showing subsets of HP1γ-interacting proteins functionally related to DNA damage response pathways. The numbers in five subsets represent proteins that are interacting with HP1γ and functions in DNA damage response. IB, immunoblot; IP, immunoprecipitate.
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Cell Signaling Technology Inc anti proliferating cell nuclear antigen pcna
    Effects of des-Arg 9 -bradykinin (DABK) and angiotensin II (ANG II) vascular smooth muscle cells (VSMC) growth. [A] Aortic VSMC from Wistar rats treated with ANG II and/or DABK at low concentration (0.1 nM) in the presence or not of losartan (LOS – 10 µM), des-arg9-leu8-bradykinin (DAL – 10 µM) and TIRON (1 mM). Bar graph shows <t>PCNA</t> expression normalized against the housekeeping protein <t>β-actin.</t> Results are represented as mean±SEM of 4 experiments. *P
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    GeneTex anti proliferating cell nuclear antigen pcna
    Effects of des-Arg 9 -bradykinin (DABK) and angiotensin II (ANG II) vascular smooth muscle cells (VSMC) growth. [A] Aortic VSMC from Wistar rats treated with ANG II and/or DABK at low concentration (0.1 nM) in the presence or not of losartan (LOS – 10 µM), des-arg9-leu8-bradykinin (DAL – 10 µM) and TIRON (1 mM). Bar graph shows <t>PCNA</t> expression normalized against the housekeeping protein <t>β-actin.</t> Results are represented as mean±SEM of 4 experiments. *P
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti proliferating cell nuclear antigen pcna
    Effects of manipulation of integrin αvβ5 activity on tumorigenesis. 3-dpi kras+ and WT larvae were treated cilengitide or ADP together with dox for 4 days. Liver size were measured and liver sections were stained for cell proliferation and apoptosis. More than 20 fish were analyzed in each group. (A) Gross morphology of larvae after 4 days of treatment (left lateral view). The WT livers were outlined. (B) Quantification of liver size. (C) IF staining of <t>PCNA</t> on liver sections. (D) Quantification of PCNA-positive cells in the liver. (E) IF staining of <t>Caspase</t> 3 on liver sections. (F) Quantification of Caspase 3-positive cells in the liver. Livers are marked by dash lines. Int indicates intestine. * P
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Upstate Biotechnology Inc anti proliferating cell nuclear antigen pcna
    <t>Cdk2</t> expression and cell proliferation are downregulated between perinatal and adult oligodendrocyte progenitor cells. Subventricular zone ( SVZ ), subcortical white matter ( SCWM ), and cerebellar white matter ( CBL ) from CNP–GFP mice were analyzed by immunostaining at P6 and P30, to assess the number of cdk2- ( A ) and <t>PCNA-expressing</t> ( B ) cells within the NG2 + /GFP + population at distinct developmental stages. Histograms represent mean ± SEM. Total GFP + cells counted ranged between 483 and 641 at P6, and between 409 and 455 at P30.
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson anti proliferating cell nuclear antigen pcna
    <t>Cdk2</t> expression and cell proliferation are downregulated between perinatal and adult oligodendrocyte progenitor cells. Subventricular zone ( SVZ ), subcortical white matter ( SCWM ), and cerebellar white matter ( CBL ) from CNP–GFP mice were analyzed by immunostaining at P6 and P30, to assess the number of cdk2- ( A ) and <t>PCNA-expressing</t> ( B ) cells within the NG2 + /GFP + population at distinct developmental stages. Histograms represent mean ± SEM. Total GFP + cells counted ranged between 483 and 641 at P6, and between 409 and 455 at P30.
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies anti proliferating cell nuclear antigen pcna
    Cell turnover after patch venoplasty. (A) Representative Western blot showing expression of <t>PCNA</t> , cleaved caspase‐3 and <t>GAPDH</t> in the control IVC (vein), preimplantation patch (day 0), and patch explanted at day 7 or day 30; n = 3. (B) Immunohistochemical analysis of the neointima at day 0 (left column), day 7 (middle column), or day 30 (right column). Analysis for: upper row, proliferating cell nuclear antigen ( PCNA ) (scale bar, 100 μ m); lower row, cleaved caspase‐3 (scale bar, 50 μ m). P, patch; L, lumen; N, neointima. Yellow arrow shows the positive cells. n = 4. (C) Bar graph showing neointimal proliferation index (mean number of cells counted in 4 high power fields); P
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Signet Testing anti proliferating cell nuclear antigen pcna
    Cell turnover after patch venoplasty. (A) Representative Western blot showing expression of <t>PCNA</t> , cleaved caspase‐3 and <t>GAPDH</t> in the control IVC (vein), preimplantation patch (day 0), and patch explanted at day 7 or day 30; n = 3. (B) Immunohistochemical analysis of the neointima at day 0 (left column), day 7 (middle column), or day 30 (right column). Analysis for: upper row, proliferating cell nuclear antigen ( PCNA ) (scale bar, 100 μ m); lower row, cleaved caspase‐3 (scale bar, 50 μ m). P, patch; L, lumen; N, neointima. Yellow arrow shows the positive cells. n = 4. (C) Bar graph showing neointimal proliferation index (mean number of cells counted in 4 high power fields); P
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Signet Testing, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    BioLegend anti proliferating cell nuclear antigen pcna
    Cell turnover after patch venoplasty. (A) Representative Western blot showing expression of <t>PCNA</t> , cleaved caspase‐3 and <t>GAPDH</t> in the control IVC (vein), preimplantation patch (day 0), and patch explanted at day 7 or day 30; n = 3. (B) Immunohistochemical analysis of the neointima at day 0 (left column), day 7 (middle column), or day 30 (right column). Analysis for: upper row, proliferating cell nuclear antigen ( PCNA ) (scale bar, 100 μ m); lower row, cleaved caspase‐3 (scale bar, 50 μ m). P, patch; L, lumen; N, neointima. Yellow arrow shows the positive cells. n = 4. (C) Bar graph showing neointimal proliferation index (mean number of cells counted in 4 high power fields); P
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by BioLegend, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Leinco Technologies anti proliferating cell nuclear antigen pcna
    ( A ) Immunofluorescence of tubular damage marker KIM ‐1 in WT and in Yb1 +/− mice. ( B ) Transcript numbers of chemokine CCL 5 were determined by qRT ‐ PCR in kidneys of WT and Yb1 +/− mice following I/R at different time‐points ( n = 5–10). ( C/D ) Numbers of renal infiltrating immune cells positive for Ly6G in renal medulla on day 1 and in renal cortex on days 1, 5 and 21 ( C ) and immune cells positive for <t>F4/80</t> in cortex ( D ) in Yb1 +/− compared to WT mice following I/R ( n = 5–14). ( E/F ) Representative images of Col1 immunostaining ( E ) and quantification thereof by computer‐based morphometric analyses of the positively stained cortical area (%) ( n = 5–7) ( F ). In Yb1 +/− mice, cortical Col1A1 deposition was significantly less on day 5 but more on day 21 compared to their WT littermates. ( G/H ) <t>PCNA</t> + tubular nuclei in post‐ischaemic Yb1 +/− mice in comparison with WT mice on different days ( G ) and representative images on day 21 ( n = 5–11) ( H ). ( I–L ) Tubular damage in PAS ‐stained cortical tissue in I/R groups (I) and (J/K) quantification of tubular PCNA + nuclei ( J ) and F4/80 + cells ( K ) and NGAL mRNA expression ( L ) in kidneys following shortened ischaemia (25 min.) time ( n = 4–5). Scale bars, 50 μm. Data are expressed as mean values ± S.D. * P
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Leinco Technologies, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Santa Cruz Biotechnology mouse α proliferating cell nuclear antigen pcna
    ( A ) Immunofluorescence of tubular damage marker KIM ‐1 in WT and in Yb1 +/− mice. ( B ) Transcript numbers of chemokine CCL 5 were determined by qRT ‐ PCR in kidneys of WT and Yb1 +/− mice following I/R at different time‐points ( n = 5–10). ( C/D ) Numbers of renal infiltrating immune cells positive for Ly6G in renal medulla on day 1 and in renal cortex on days 1, 5 and 21 ( C ) and immune cells positive for <t>F4/80</t> in cortex ( D ) in Yb1 +/− compared to WT mice following I/R ( n = 5–14). ( E/F ) Representative images of Col1 immunostaining ( E ) and quantification thereof by computer‐based morphometric analyses of the positively stained cortical area (%) ( n = 5–7) ( F ). In Yb1 +/− mice, cortical Col1A1 deposition was significantly less on day 5 but more on day 21 compared to their WT littermates. ( G/H ) <t>PCNA</t> + tubular nuclei in post‐ischaemic Yb1 +/− mice in comparison with WT mice on different days ( G ) and representative images on day 21 ( n = 5–11) ( H ). ( I–L ) Tubular damage in PAS ‐stained cortical tissue in I/R groups (I) and (J/K) quantification of tubular PCNA + nuclei ( J ) and F4/80 + cells ( K ) and NGAL mRNA expression ( L ) in kidneys following shortened ischaemia (25 min.) time ( n = 4–5). Scale bars, 50 μm. Data are expressed as mean values ± S.D. * P
    Mouse α Proliferating Cell Nuclear Antigen Pcna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The function of heterochromatin protein 1γ (HP1γ) in the DNA damage response pathway. (A) Bloom syndrome protein (BLM) and proliferating cell nuclear antigen (PCNA) were associated with endogenous HP1γ proteins. BLM and PCNA were coimmunoprecipitated with anti-HP1γ antibodies using HEK293T cell lysates and immunoblotted with indicated antibodies. (B) HeLa cells were treated with 50 μM of etoposide or vehicle (dimethyl sulfoxide, DMSO) for 6 hours and immunostained with the indicated antibodies. (C) HeLa cells were transfected with pSUPER-siHP1γ and immunostained with the indicated antibodies. (D) Nonproportional Venn diagram showing subsets of HP1γ-interacting proteins functionally related to DNA damage response pathways. The numbers in five subsets represent proteins that are interacting with HP1γ and functions in DNA damage response. IB, immunoblot; IP, immunoprecipitate.

    Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

    Article Title: Interactome Analysis Reveals that Heterochromatin Protein 1γ (HP1γ) Is Associated with the DNA Damage Response Pathway

    doi: 10.4143/crt.2014.294

    Figure Lengend Snippet: The function of heterochromatin protein 1γ (HP1γ) in the DNA damage response pathway. (A) Bloom syndrome protein (BLM) and proliferating cell nuclear antigen (PCNA) were associated with endogenous HP1γ proteins. BLM and PCNA were coimmunoprecipitated with anti-HP1γ antibodies using HEK293T cell lysates and immunoblotted with indicated antibodies. (B) HeLa cells were treated with 50 μM of etoposide or vehicle (dimethyl sulfoxide, DMSO) for 6 hours and immunostained with the indicated antibodies. (C) HeLa cells were transfected with pSUPER-siHP1γ and immunostained with the indicated antibodies. (D) Nonproportional Venn diagram showing subsets of HP1γ-interacting proteins functionally related to DNA damage response pathways. The numbers in five subsets represent proteins that are interacting with HP1γ and functions in DNA damage response. IB, immunoblot; IP, immunoprecipitate.

    Article Snippet: The antibodies used for immunoblotting were as follows: anti-phospho-H3 Ser 10 (EMD Millipore), anti-Bloom syndrome protein (BLM) (Abcam, Cambridge, MA), and anti-proliferating cell nuclear antigen (PCNA) (Abcam).

    Techniques: Transfection

    Effects of XS-5 and XS-6 in HCC ex vivo tumor models. (a) Balb/c nude mice bearing Huh-7 HCC xenograft tumors were cut into small pieces of ∼2 mm, and each piece of tumor was maintained in culture media. Tumor spheroids cultured from xenograft tumor tissues were treated with XS-5 and XS-6 (100 μ g/ml) for 5 days. Immunostaining and hematoxylin and eosin staining for PCNA and cleaved caspase-3 were carried out. (b) Tumor spheroids were excised and processed for immunofluorescence for p-AKT and p-mTOR. Images were captured at 400X magnification. Data are represented as the mean ± SD ( ∗∗∗ p

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Apoptotic Effects of Xanthium strumarium via PI3K/AKT/mTOR Pathway in Hepatocellular Carcinoma

    doi: 10.1155/2019/2176701

    Figure Lengend Snippet: Effects of XS-5 and XS-6 in HCC ex vivo tumor models. (a) Balb/c nude mice bearing Huh-7 HCC xenograft tumors were cut into small pieces of ∼2 mm, and each piece of tumor was maintained in culture media. Tumor spheroids cultured from xenograft tumor tissues were treated with XS-5 and XS-6 (100 μ g/ml) for 5 days. Immunostaining and hematoxylin and eosin staining for PCNA and cleaved caspase-3 were carried out. (b) Tumor spheroids were excised and processed for immunofluorescence for p-AKT and p-mTOR. Images were captured at 400X magnification. Data are represented as the mean ± SD ( ∗∗∗ p

    Article Snippet: The sections were blocked with blocking solution including normal goat or horse serum for 40 min. Next, the sections were incubated overnight with anti-proliferating cell nuclear antigen (PCNA) (Abcam) and cleaved caspase-3 (Cell Signaling Technologies) at 4°C.

    Techniques: Ex Vivo, Mouse Assay, Cell Culture, Immunostaining, Staining, Immunofluorescence

    Effects of des-Arg 9 -bradykinin (DABK) and angiotensin II (ANG II) vascular smooth muscle cells (VSMC) growth. [A] Aortic VSMC from Wistar rats treated with ANG II and/or DABK at low concentration (0.1 nM) in the presence or not of losartan (LOS – 10 µM), des-arg9-leu8-bradykinin (DAL – 10 µM) and TIRON (1 mM). Bar graph shows PCNA expression normalized against the housekeeping protein β-actin. Results are represented as mean±SEM of 4 experiments. *P

    Journal: PLoS ONE

    Article Title: An Interaction of Renin-Angiotensin and Kallikrein-Kinin Systems Contributes to Vascular Hypertrophy in Angiotensin II-Induced Hypertension: In Vivo and In Vitro Studies

    doi: 10.1371/journal.pone.0111117

    Figure Lengend Snippet: Effects of des-Arg 9 -bradykinin (DABK) and angiotensin II (ANG II) vascular smooth muscle cells (VSMC) growth. [A] Aortic VSMC from Wistar rats treated with ANG II and/or DABK at low concentration (0.1 nM) in the presence or not of losartan (LOS – 10 µM), des-arg9-leu8-bradykinin (DAL – 10 µM) and TIRON (1 mM). Bar graph shows PCNA expression normalized against the housekeeping protein β-actin. Results are represented as mean±SEM of 4 experiments. *P

    Article Snippet: In other series of experiments membranes from VSMC were then incubated with anti-B1R (1∶1000) (Santa Cruz Biotechnology, Santa Cruz, California, USA) or anti-proliferating-cell nuclear antigen (PCNA) (Cell Signaling) and also anti-β-actin (Santa Cruz Biotechnology, Santa Cruz, California, USA) at 4°C. β-actin was used as a housekeeping protein.

    Techniques: Concentration Assay, Expressing

    Effects of manipulation of integrin αvβ5 activity on tumorigenesis. 3-dpi kras+ and WT larvae were treated cilengitide or ADP together with dox for 4 days. Liver size were measured and liver sections were stained for cell proliferation and apoptosis. More than 20 fish were analyzed in each group. (A) Gross morphology of larvae after 4 days of treatment (left lateral view). The WT livers were outlined. (B) Quantification of liver size. (C) IF staining of PCNA on liver sections. (D) Quantification of PCNA-positive cells in the liver. (E) IF staining of Caspase 3 on liver sections. (F) Quantification of Caspase 3-positive cells in the liver. Livers are marked by dash lines. Int indicates intestine. * P

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Activation of Hepatic Stellate Cells During Liver Carcinogenesis Requires Fibrinogen/Integrin αvβ5 in Zebrafish

    doi: 10.1016/j.neo.2018.02.002

    Figure Lengend Snippet: Effects of manipulation of integrin αvβ5 activity on tumorigenesis. 3-dpi kras+ and WT larvae were treated cilengitide or ADP together with dox for 4 days. Liver size were measured and liver sections were stained for cell proliferation and apoptosis. More than 20 fish were analyzed in each group. (A) Gross morphology of larvae after 4 days of treatment (left lateral view). The WT livers were outlined. (B) Quantification of liver size. (C) IF staining of PCNA on liver sections. (D) Quantification of PCNA-positive cells in the liver. (E) IF staining of Caspase 3 on liver sections. (F) Quantification of Caspase 3-positive cells in the liver. Livers are marked by dash lines. Int indicates intestine. * P

    Article Snippet: The primary antibodies used included anti-proliferating cell nuclear antigen (PCNA) (FL-261; Santa Cruz Biotechnology, Dallas, TX), anti–Caspase 3 (C92-065; BD Biosciences, Singapore), anti–collagen I (ab23730; Abcam), anti-laminin (L9393; Sigma), anti-glial fibrillary acidic protein (Gfap) (154474; Abcam), anti-a-Sma (ab15734; Abcam, Singapore), anti-fibrinogen (sc69775; Santa Cruze) and anti-Tgfb1 (04-953; EMD Millipore, Billerica, MA).

    Techniques: Activity Assay, Staining, Fluorescence In Situ Hybridization

    Characterization of kras V12 -induced hepatocarcinogenesis in zebrafish larvae. 3-dpi kras+ or WT larvae were treated with 20 μg/ml for 4 days. More than 20 larvae were analyzed in each group. (A) Gross morphology of kras+ or WT larvae after 4 days induction (left lateral view). kras V12 expressing liver is marked by GFP expression and WT liver is outlined. Quantification of 2D liver size was shown in the right panel. (B-E) IF staining of PCNA (B), Caspase 3 (C), Collagen I (D) or Laminin (E) on liver sections of kras+ and WT larvae as indicated. Livers are marked by dash lines. Int indicates intestine. Quantifications of staining signals based on percentages of liver area are shown in the right panels. (F) IF co-staining of GFAP (red) and a-Sma (blue) on liver sections of kras+ and WT larvae as indicated. Quantifications of total HSC density based on Gfap+ cells and ratio of activated HSCs based on a-SMA staining in liver sections are presented on the right. * P

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Activation of Hepatic Stellate Cells During Liver Carcinogenesis Requires Fibrinogen/Integrin αvβ5 in Zebrafish

    doi: 10.1016/j.neo.2018.02.002

    Figure Lengend Snippet: Characterization of kras V12 -induced hepatocarcinogenesis in zebrafish larvae. 3-dpi kras+ or WT larvae were treated with 20 μg/ml for 4 days. More than 20 larvae were analyzed in each group. (A) Gross morphology of kras+ or WT larvae after 4 days induction (left lateral view). kras V12 expressing liver is marked by GFP expression and WT liver is outlined. Quantification of 2D liver size was shown in the right panel. (B-E) IF staining of PCNA (B), Caspase 3 (C), Collagen I (D) or Laminin (E) on liver sections of kras+ and WT larvae as indicated. Livers are marked by dash lines. Int indicates intestine. Quantifications of staining signals based on percentages of liver area are shown in the right panels. (F) IF co-staining of GFAP (red) and a-Sma (blue) on liver sections of kras+ and WT larvae as indicated. Quantifications of total HSC density based on Gfap+ cells and ratio of activated HSCs based on a-SMA staining in liver sections are presented on the right. * P

    Article Snippet: The primary antibodies used included anti-proliferating cell nuclear antigen (PCNA) (FL-261; Santa Cruz Biotechnology, Dallas, TX), anti–Caspase 3 (C92-065; BD Biosciences, Singapore), anti–collagen I (ab23730; Abcam), anti-laminin (L9393; Sigma), anti-glial fibrillary acidic protein (Gfap) (154474; Abcam), anti-a-Sma (ab15734; Abcam, Singapore), anti-fibrinogen (sc69775; Santa Cruze) and anti-Tgfb1 (04-953; EMD Millipore, Billerica, MA).

    Techniques: Expressing, Staining

    Western blot analysis of the cell cycle signaling proteins. The lungs of mice fed a low Pi diet (0.1% Pi) or a normal (0.5% Pi) diet for 4 weeks. (A) The expressions of p53, p21 and p27 protein in lung. (B) The expressions of cyclin D3, cyclin-dependent kinase 4 (CDK4) and proliferating cell nuclear antigen (PCNA) protein in lung. (C, D) The bands-of-interests were further analyzed by using a densitometer. (E) Immunohistochemical measurement of PCNA in the lung. The dark brown color indicates the PCNA expression (scale bar = 100 µm). (F) Comparison of the PCNA labeling index in the lungs. p values ( * p

    Journal: Journal of Veterinary Science

    Article Title: Low dietary inorganic phosphate affects the lung growth of developing mice

    doi: 10.4142/jvs.2009.10.2.105

    Figure Lengend Snippet: Western blot analysis of the cell cycle signaling proteins. The lungs of mice fed a low Pi diet (0.1% Pi) or a normal (0.5% Pi) diet for 4 weeks. (A) The expressions of p53, p21 and p27 protein in lung. (B) The expressions of cyclin D3, cyclin-dependent kinase 4 (CDK4) and proliferating cell nuclear antigen (PCNA) protein in lung. (C, D) The bands-of-interests were further analyzed by using a densitometer. (E) Immunohistochemical measurement of PCNA in the lung. The dark brown color indicates the PCNA expression (scale bar = 100 µm). (F) Comparison of the PCNA labeling index in the lungs. p values ( * p

    Article Snippet: Anti-phospho-Akt (Thr308), anti-eukaryotic initiation factor 4E binding protein 1 (4E-BP1), anti-phospho-4E-BP1, anti-cyclin D3, anti-cyclin-dependent kinase 4 (CDK4), anti-proliferating cell nuclear antigen (PCNA), anti-p53, anti-p27, anti-p21 and anti-FGF-2, anti-α-tubulin antibodies were purchased from Santa Cruz Biotechnology (USA).

    Techniques: Western Blot, Mouse Assay, Immunohistochemistry, Expressing, Labeling

    Cdk2 expression and cell proliferation are downregulated between perinatal and adult oligodendrocyte progenitor cells. Subventricular zone ( SVZ ), subcortical white matter ( SCWM ), and cerebellar white matter ( CBL ) from CNP–GFP mice were analyzed by immunostaining at P6 and P30, to assess the number of cdk2- ( A ) and PCNA-expressing ( B ) cells within the NG2 + /GFP + population at distinct developmental stages. Histograms represent mean ± SEM. Total GFP + cells counted ranged between 483 and 641 at P6, and between 409 and 455 at P30.

    Journal: The Journal of Neuroscience

    Article Title: Cyclin-Dependent Kinase-2 Controls Oligodendrocyte Progenitor Cell Cycle Progression and Is Downregulated in Adult Oligodendrocyte Progenitors

    doi: 10.1523/JNEUROSCI.22-19-08553.2002

    Figure Lengend Snippet: Cdk2 expression and cell proliferation are downregulated between perinatal and adult oligodendrocyte progenitor cells. Subventricular zone ( SVZ ), subcortical white matter ( SCWM ), and cerebellar white matter ( CBL ) from CNP–GFP mice were analyzed by immunostaining at P6 and P30, to assess the number of cdk2- ( A ) and PCNA-expressing ( B ) cells within the NG2 + /GFP + population at distinct developmental stages. Histograms represent mean ± SEM. Total GFP + cells counted ranged between 483 and 641 at P6, and between 409 and 455 at P30.

    Article Snippet: Anti-cdk2 (1:200, clone M2, sc-163; Santa Cruz Biotechnology), anti- proliferating cell nuclear antigen (PCNA) (1:50, 05–347; Upstate Biotechnology, Lake Placid, NY), and NG2 (1:1000, AB5320; Chemicon, Temecula, CA) primary antibodies were diluted using carrier solution (1% BSA, 0.3% Triton X-100 in 1× PBS).

    Techniques: Expressing, Mouse Assay, Immunostaining

    Proliferating GFP + oligodendroglial cells express sustained levels of cdk2 during development. Subventricular zone ( SVZ ), subcortical white matter ( SCWM ), and cerebellar white matter ( CBL ) from CNP–GFP mice were analyzed by immunostaining at P6 and P30, to assess the number of PCNA- and cdk2-co-expressing cells within the GFP + population at distinct developmental stages. A shows the percentage of PCNA + cells also expressing cdk2, whereas B indicates the percentage of cdk2 + cells also expressing PCNA. Histograms represent mean ± SEM. Total GFP + cells counted ranged between 483 and 597 at P6 and between 409 and 455 at P30.

    Journal: The Journal of Neuroscience

    Article Title: Cyclin-Dependent Kinase-2 Controls Oligodendrocyte Progenitor Cell Cycle Progression and Is Downregulated in Adult Oligodendrocyte Progenitors

    doi: 10.1523/JNEUROSCI.22-19-08553.2002

    Figure Lengend Snippet: Proliferating GFP + oligodendroglial cells express sustained levels of cdk2 during development. Subventricular zone ( SVZ ), subcortical white matter ( SCWM ), and cerebellar white matter ( CBL ) from CNP–GFP mice were analyzed by immunostaining at P6 and P30, to assess the number of PCNA- and cdk2-co-expressing cells within the GFP + population at distinct developmental stages. A shows the percentage of PCNA + cells also expressing cdk2, whereas B indicates the percentage of cdk2 + cells also expressing PCNA. Histograms represent mean ± SEM. Total GFP + cells counted ranged between 483 and 597 at P6 and between 409 and 455 at P30.

    Article Snippet: Anti-cdk2 (1:200, clone M2, sc-163; Santa Cruz Biotechnology), anti- proliferating cell nuclear antigen (PCNA) (1:50, 05–347; Upstate Biotechnology, Lake Placid, NY), and NG2 (1:1000, AB5320; Chemicon, Temecula, CA) primary antibodies were diluted using carrier solution (1% BSA, 0.3% Triton X-100 in 1× PBS).

    Techniques: Mouse Assay, Immunostaining, Expressing

    Cell turnover after patch venoplasty. (A) Representative Western blot showing expression of PCNA , cleaved caspase‐3 and GAPDH in the control IVC (vein), preimplantation patch (day 0), and patch explanted at day 7 or day 30; n = 3. (B) Immunohistochemical analysis of the neointima at day 0 (left column), day 7 (middle column), or day 30 (right column). Analysis for: upper row, proliferating cell nuclear antigen ( PCNA ) (scale bar, 100 μ m); lower row, cleaved caspase‐3 (scale bar, 50 μ m). P, patch; L, lumen; N, neointima. Yellow arrow shows the positive cells. n = 4. (C) Bar graph showing neointimal proliferation index (mean number of cells counted in 4 high power fields); P

    Journal: Physiological Reports

    Article Title: Pericardial patch venoplasty heals via attraction of venous progenitor cells. Pericardial patch venoplasty heals via attraction of venous progenitor cells

    doi: 10.14814/phy2.12841

    Figure Lengend Snippet: Cell turnover after patch venoplasty. (A) Representative Western blot showing expression of PCNA , cleaved caspase‐3 and GAPDH in the control IVC (vein), preimplantation patch (day 0), and patch explanted at day 7 or day 30; n = 3. (B) Immunohistochemical analysis of the neointima at day 0 (left column), day 7 (middle column), or day 30 (right column). Analysis for: upper row, proliferating cell nuclear antigen ( PCNA ) (scale bar, 100 μ m); lower row, cleaved caspase‐3 (scale bar, 50 μ m). P, patch; L, lumen; N, neointima. Yellow arrow shows the positive cells. n = 4. (C) Bar graph showing neointimal proliferation index (mean number of cells counted in 4 high power fields); P

    Article Snippet: Primary and secondary antibodies Primary antibodies included: anti‐α ‐actin (Abcam, ab5694; IHC and IF, 1:100; WB, 1:1000); anti‐cleaved Caspase‐3 (Cell Signaling #9661; IHC, 1:50; WB, 1:1000); anti‐CD31 (Abcam, ab28364; IHC and IF, 1:50); anti‐CD34 (R & D, AF4117; IF, 1:100; WB, 1:1000); anti‐CD45 (Abcam, ab10558; IHC, 1:50; WB, 1:1000); anti‐CD68 (ED1; Abcam, ab31630; IHC, 1:200; WB, 1:1000); anti‐ COUP‐TFII (Novus biologicals, NBP1‐67885; IHC, 1:100); anti‐Eph‐B4 (Santa Cruz, sc‐5536; IF, 1:50); anti‐dll‐4 (santa cruz, sc‐18640, 1:50); anti‐Eph‐B4 (Abcam, ab76657; IHC and IF, 1:50); anti‐Ephrin‐B2 (Novus, NBP1‐48610; IHC, 1:50); anti‐Eph‐B4 (R & D, AF446; WB, 1:1000); anti‐GAPDH (Cell Signaling, 14C10; WB, 1:2000); anti‐Ki67 (Abcam, ab15580; IHC, 1:100); anti‐proliferating cell nuclear antigen (PCNA) (Dako monoclonal mouse Anti‐PCNA; IHC and IF, 1:100; WB, 1:1000); anti‐VEGFR2 (ABCAM, ab2349; IF, 1:100; WB,1:1000); and anti‐vimentin (Abcam, ab8978; IHC, 1:50; WB, 1:1000).

    Techniques: Western Blot, Expressing, Immunohistochemistry

    spd have a reduction of Fgf signaling in the periventricular nucleus. a–j , High-magnification images of coronal sections through the adult zebrafish hypothalamus, showing dusp6 in situ hybridization ( a , b ), pERK immunocytochemistry ( c , d ), PCNA immunocytochemistry ( e , f ), TUNEL labeling ( g , h ), and Nissl staining ( i , j ). dusp6 and pERK are reduced in the PVN of spd ( b , d ) compared with siblings ( a , c ). PCNA and TUNEL labeling is similar between wild-type ( e , g ) and spd ( f , h ). The Nissl-stained PVN of spd ( j ) appears larger than wild-type ( i ). Arrowheads in a–d highlight the PVN. Arrowheads in e and f point to PCNA-positive cells. k , Diagram depicting the position of all sections in the zebrafish brain. l–o , The expression of mao ( l , m ) and slc6a4b ( n , o ) in the periventricular nucleus of both wild-type ( l , n ) and spd ( m , o ). Expression levels are similar in both genotypes. p–s , The expression of pomcb ( p , q ) and nvpfl ( r , s ) in the preoptic area of both wild-type ( p , r ) and spd ( q , s ). Expression levels are similar in both genotypes. PM, magnocellular preoptic nucleus; PPp, parvocellular preoptic nucleus posterior part; SC, suprachiasmatic nucleus.

    Journal: The Journal of Neuroscience

    Article Title: Modulation of Fgfr1a Signaling in Zebrafish Reveals a Genetic Basis for the Aggression–Boldness Syndrome

    doi: 10.1523/JNEUROSCI.2892-11.2011

    Figure Lengend Snippet: spd have a reduction of Fgf signaling in the periventricular nucleus. a–j , High-magnification images of coronal sections through the adult zebrafish hypothalamus, showing dusp6 in situ hybridization ( a , b ), pERK immunocytochemistry ( c , d ), PCNA immunocytochemistry ( e , f ), TUNEL labeling ( g , h ), and Nissl staining ( i , j ). dusp6 and pERK are reduced in the PVN of spd ( b , d ) compared with siblings ( a , c ). PCNA and TUNEL labeling is similar between wild-type ( e , g ) and spd ( f , h ). The Nissl-stained PVN of spd ( j ) appears larger than wild-type ( i ). Arrowheads in a–d highlight the PVN. Arrowheads in e and f point to PCNA-positive cells. k , Diagram depicting the position of all sections in the zebrafish brain. l–o , The expression of mao ( l , m ) and slc6a4b ( n , o ) in the periventricular nucleus of both wild-type ( l , n ) and spd ( m , o ). Expression levels are similar in both genotypes. p–s , The expression of pomcb ( p , q ) and nvpfl ( r , s ) in the preoptic area of both wild-type ( p , r ) and spd ( q , s ). Expression levels are similar in both genotypes. PM, magnocellular preoptic nucleus; PPp, parvocellular preoptic nucleus posterior part; SC, suprachiasmatic nucleus.

    Article Snippet: The following antibodies were used: anti-phosphorylated extracellular signal-regulated kinase (pERK) [mitogen-activated protein kinase (MAPK); 1:1000; Sigma], anti- proliferating cell nuclear antigen (PCNA) (1:1000; Dako), anti-gonadotrophin releasing hormone (GnRH) (1:500; Sigma), and anti-histamine (1:2000; gift from Dr. P. Panula, University of Helsinki, Helsinki, Finland).

    Techniques: In Situ Hybridization, Immunocytochemistry, TUNEL Assay, Labeling, Staining, Expressing

    ( A ) Immunofluorescence of tubular damage marker KIM ‐1 in WT and in Yb1 +/− mice. ( B ) Transcript numbers of chemokine CCL 5 were determined by qRT ‐ PCR in kidneys of WT and Yb1 +/− mice following I/R at different time‐points ( n = 5–10). ( C/D ) Numbers of renal infiltrating immune cells positive for Ly6G in renal medulla on day 1 and in renal cortex on days 1, 5 and 21 ( C ) and immune cells positive for F4/80 in cortex ( D ) in Yb1 +/− compared to WT mice following I/R ( n = 5–14). ( E/F ) Representative images of Col1 immunostaining ( E ) and quantification thereof by computer‐based morphometric analyses of the positively stained cortical area (%) ( n = 5–7) ( F ). In Yb1 +/− mice, cortical Col1A1 deposition was significantly less on day 5 but more on day 21 compared to their WT littermates. ( G/H ) PCNA + tubular nuclei in post‐ischaemic Yb1 +/− mice in comparison with WT mice on different days ( G ) and representative images on day 21 ( n = 5–11) ( H ). ( I–L ) Tubular damage in PAS ‐stained cortical tissue in I/R groups (I) and (J/K) quantification of tubular PCNA + nuclei ( J ) and F4/80 + cells ( K ) and NGAL mRNA expression ( L ) in kidneys following shortened ischaemia (25 min.) time ( n = 4–5). Scale bars, 50 μm. Data are expressed as mean values ± S.D. * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YB‐1 orchestrates onset and resolution of renal inflammation via IL10 gene regulation

    doi: 10.1111/jcmm.13260

    Figure Lengend Snippet: ( A ) Immunofluorescence of tubular damage marker KIM ‐1 in WT and in Yb1 +/− mice. ( B ) Transcript numbers of chemokine CCL 5 were determined by qRT ‐ PCR in kidneys of WT and Yb1 +/− mice following I/R at different time‐points ( n = 5–10). ( C/D ) Numbers of renal infiltrating immune cells positive for Ly6G in renal medulla on day 1 and in renal cortex on days 1, 5 and 21 ( C ) and immune cells positive for F4/80 in cortex ( D ) in Yb1 +/− compared to WT mice following I/R ( n = 5–14). ( E/F ) Representative images of Col1 immunostaining ( E ) and quantification thereof by computer‐based morphometric analyses of the positively stained cortical area (%) ( n = 5–7) ( F ). In Yb1 +/− mice, cortical Col1A1 deposition was significantly less on day 5 but more on day 21 compared to their WT littermates. ( G/H ) PCNA + tubular nuclei in post‐ischaemic Yb1 +/− mice in comparison with WT mice on different days ( G ) and representative images on day 21 ( n = 5–11) ( H ). ( I–L ) Tubular damage in PAS ‐stained cortical tissue in I/R groups (I) and (J/K) quantification of tubular PCNA + nuclei ( J ) and F4/80 + cells ( K ) and NGAL mRNA expression ( L ) in kidneys following shortened ischaemia (25 min.) time ( n = 4–5). Scale bars, 50 μm. Data are expressed as mean values ± S.D. * P

    Article Snippet: Renal tissues were stained using the following primary antibodies: anti‐human Col1A1 (Southern Biotech, Birmingham, AL), anti‐Kim1 (R & D systems, USA), anti‐mouse F4/80 (Serotec, Düsseldorf, Germany), anti‐proliferating cell nuclear antigen (PCNA) (Leinco Technologies, St. Louis, MO) and anti‐Ly6G (BD Biosciences, San Jose, CA).

    Techniques: Immunofluorescence, Marker, Mouse Assay, Quantitative RT-PCR, Immunostaining, Staining, Expressing