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  • 94
    Millipore pcbs
    (a) Total Q factors of the functional microsphere versus the self-assembled polymer layer thickness (t). The experimental data are shown as triangles (for <t>PAH/PB)</t> and dots (for <t>PAH/PCBS).</t> The theoretical fittings are performed using 1/ Q = 1/ Q coupling + A × t α , where Q coupling , A , and α are fitting constants. (b) The relationship between film thickness t and Q film . The fitted values are given by1/ Q film = A × t α . The experimental data are obtained using 1/ Q film = 1/ Q - 1/ Q coupling. .
    Pcbs, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcbs/product/Millipore
    Average 94 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
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    92
    Xenobiotics pcbs
    (a) Total Q factors of the functional microsphere versus the self-assembled polymer layer thickness (t). The experimental data are shown as triangles (for <t>PAH/PB)</t> and dots (for <t>PAH/PCBS).</t> The theoretical fittings are performed using 1/ Q = 1/ Q coupling + A × t α , where Q coupling , A , and α are fitting constants. (b) The relationship between film thickness t and Q film . The fitted values are given by1/ Q film = A × t α . The experimental data are obtained using 1/ Q film = 1/ Q - 1/ Q coupling. .
    Pcbs, supplied by Xenobiotics, used in various techniques. Bioz Stars score: 92/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 79 article reviews
    Price from $9.99 to $1999.99
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    89
    Kanto Chemical pcb 126
    (a) Uptake of colchicine in KB3-1 expressing with wild-type human P-glycoprotein (KB3-His 61 , filled circles), mutant P-glycoprotein in which His 61 was replaced with phenylalanine (KB3-Phe 61 , open squares) and serine (KB3-Ser 61 , filled squares), and transfected with transfection vector (KB3-Vec, open circles). (b) The ratios between the accumulations of colchicine with and without cyclosporine A (CYA) and (c) <t>PCB-126</t> (with/without CYA or PCB-126) in KB3-Vec (open columns), KB3-His 61 (cross-hatched columns), KB3-Phe 61 (hatched columns), and KB3-Ser 61 (filled columns). ∗ , P
    Pcb 126, supplied by Kanto Chemical, used in various techniques. Bioz Stars score: 89/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcb 126/product/Kanto Chemical
    Average 89 stars, based on 17 article reviews
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    90
    Dr Ehrenstorfer GmbH pcb congeners
    Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of <t>PCB</t> congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of <t>PCBs</t> released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p
    Pcb Congeners, supplied by Dr Ehrenstorfer GmbH, used in various techniques. Bioz Stars score: 90/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies pcb congeners
    Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of <t>PCB</t> congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of <t>PCBs</t> released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p
    Pcb Congeners, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Accustandard Inc pcb 153
    Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of <t>PCB</t> congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of <t>PCBs</t> released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p
    Pcb 153, supplied by Accustandard Inc, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcb 153/product/Accustandard Inc
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    91
    Accustandard Inc pcb 126
    Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of <t>PCB</t> congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of <t>PCBs</t> released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p
    Pcb 126, supplied by Accustandard Inc, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcb 126/product/Accustandard Inc
    Average 91 stars, based on 13 article reviews
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    pcb 126 - by Bioz Stars, 2020-09
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    91
    DuPont de Nemours flexible pcb
    Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of <t>PCB</t> congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of <t>PCBs</t> released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p
    Flexible Pcb, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 10 article reviews
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    93
    PCB Piezotronics pcb microphone
    Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of <t>PCB</t> congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of <t>PCBs</t> released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p
    Pcb Microphone, supplied by PCB Piezotronics, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Cerilliant Corporation pcb 153
    Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of <t>PCB</t> congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of <t>PCBs</t> released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p
    Pcb 153, supplied by Cerilliant Corporation, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 7 article reviews
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    91
    DuPont de Nemours pcb foil
    Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of <t>PCB</t> congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of <t>PCBs</t> released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p
    Pcb Foil, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Chiron AS pcb 126
    Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of <t>PCB</t> congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of <t>PCBs</t> released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p
    Pcb 126, supplied by Chiron AS, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcb 126/product/Chiron AS
    Average 92 stars, based on 10 article reviews
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    pcb 126 - by Bioz Stars, 2020-09
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    88
    Kanto Chemical co pcbs
    Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of <t>PCB</t> congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of <t>PCBs</t> released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p
    Co Pcbs, supplied by Kanto Chemical, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/co pcbs/product/Kanto Chemical
    Average 88 stars, based on 7 article reviews
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    co pcbs - by Bioz Stars, 2020-09
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    88
    Accustandard Inc pcb 77
    The percentage of initial molar concentrations for total PCB (orange), PCB 52 (green), <t>PCB</t> 77 (blue), and PCB 153(purple) after 12 weeks and 24 weeks incubation in unplanted soil (UP), switchgrass treated soil (SG), unplanted with bioaugmentaion soil
    Pcb 77, supplied by Accustandard Inc, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcb 77/product/Accustandard Inc
    Average 88 stars, based on 5 article reviews
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    pcb 77 - by Bioz Stars, 2020-09
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    93
    Biochrom double beam biochrom libra pcb 1500 uv vis spectrophotometer
    The percentage of initial molar concentrations for total PCB (orange), PCB 52 (green), <t>PCB</t> 77 (blue), and PCB 153(purple) after 12 weeks and 24 weeks incubation in unplanted soil (UP), switchgrass treated soil (SG), unplanted with bioaugmentaion soil
    Double Beam Biochrom Libra Pcb 1500 Uv Vis Spectrophotometer, supplied by Biochrom, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 29 article reviews
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    88
    Tekscan Inc pcb based sensor
    The percentage of initial molar concentrations for total PCB (orange), PCB 52 (green), <t>PCB</t> 77 (blue), and PCB 153(purple) after 12 weeks and 24 weeks incubation in unplanted soil (UP), switchgrass treated soil (SG), unplanted with bioaugmentaion soil
    Pcb Based Sensor, supplied by Tekscan Inc, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore pcb 153
    <t>PCB</t> 153 causes NF-κB mediated intestinal inflammation and increases gut permeability Mice were orally exposed once per day to PCB 153 (300μmol/kg) or vehicle for 2 days, following IP injections of the NF-κB inhibitor PDTC or control (PBS). (A.) IECs were isolated, RNA was extracted and RT-qPCR run for IL-6 and (B.) TNFα. N=5 mice per group. Two-way AVOVA with Tukey’s multiple comparison test. **P
    Pcb 153, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 26 article reviews
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    pcb 153 - by Bioz Stars, 2020-09
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    91
    Accustandard Inc pcbs
    <t>PCB</t> 153 causes NF-κB mediated intestinal inflammation and increases gut permeability Mice were orally exposed once per day to PCB 153 (300μmol/kg) or vehicle for 2 days, following IP injections of the NF-κB inhibitor PDTC or control (PBS). (A.) IECs were isolated, RNA was extracted and RT-qPCR run for IL-6 and (B.) TNFα. N=5 mice per group. Two-way AVOVA with Tukey’s multiple comparison test. **P
    Pcbs, supplied by Accustandard Inc, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcbs/product/Accustandard Inc
    Average 91 stars, based on 6 article reviews
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    pcbs - by Bioz Stars, 2020-09
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    93
    Wellington Laboratories pcbs
    Concentrations of Σ 17 <t>PCDD/Fs</t> and Σ 19 <t>PCBs</t> in agricultural-grade (n = 10) and analytical-grade (n = 4) CuSO 4 . The box upper and under lines represent the 25th and 75th percentiles, respectively, and the three horizontal bars represent the 5th, 50th, and 95th percentiles. * represent the 1% and 99%, □ represent mean values.
    Pcbs, supplied by Wellington Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcbs/product/Wellington Laboratories
    Average 93 stars, based on 36 article reviews
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    pcbs - by Bioz Stars, 2020-09
    93/100 stars
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    93
    Wellington Laboratories oh pcbs
    Concentrations of Σ 17 <t>PCDD/Fs</t> and Σ 19 <t>PCBs</t> in agricultural-grade (n = 10) and analytical-grade (n = 4) CuSO 4 . The box upper and under lines represent the 25th and 75th percentiles, respectively, and the three horizontal bars represent the 5th, 50th, and 95th percentiles. * represent the 1% and 99%, □ represent mean values.
    Oh Pcbs, supplied by Wellington Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    oh pcbs - by Bioz Stars, 2020-09
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    89
    Accustandard Inc pcb standards
    Concentrations of Σ 17 <t>PCDD/Fs</t> and Σ 19 <t>PCBs</t> in agricultural-grade (n = 10) and analytical-grade (n = 4) CuSO 4 . The box upper and under lines represent the 25th and 75th percentiles, respectively, and the three horizontal bars represent the 5th, 50th, and 95th percentiles. * represent the 1% and 99%, □ represent mean values.
    Pcb Standards, supplied by Accustandard Inc, used in various techniques. Bioz Stars score: 89/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 26 article reviews
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    pcb standards - by Bioz Stars, 2020-09
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    91
    PCB Piezotronics pcb piezotronics accelerometer
    Concentrations of Σ 17 <t>PCDD/Fs</t> and Σ 19 <t>PCBs</t> in agricultural-grade (n = 10) and analytical-grade (n = 4) CuSO 4 . The box upper and under lines represent the 25th and 75th percentiles, respectively, and the three horizontal bars represent the 5th, 50th, and 95th percentiles. * represent the 1% and 99%, □ represent mean values.
    Pcb Piezotronics Accelerometer, supplied by PCB Piezotronics, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    PCB Piezotronics pcb signal conditioner
    Concentrations of Σ 17 <t>PCDD/Fs</t> and Σ 19 <t>PCBs</t> in agricultural-grade (n = 10) and analytical-grade (n = 4) CuSO 4 . The box upper and under lines represent the 25th and 75th percentiles, respectively, and the three horizontal bars represent the 5th, 50th, and 95th percentiles. * represent the 1% and 99%, □ represent mean values.
    Pcb Signal Conditioner, supplied by PCB Piezotronics, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    B. Braun pcb
    Concentrations of Σ 17 <t>PCDD/Fs</t> and Σ 19 <t>PCBs</t> in agricultural-grade (n = 10) and analytical-grade (n = 4) CuSO 4 . The box upper and under lines represent the 25th and 75th percentiles, respectively, and the three horizontal bars represent the 5th, 50th, and 95th percentiles. * represent the 1% and 99%, □ represent mean values.
    Pcb, supplied by B. Braun, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Frontier Scientific pcb
    Concentrations of Σ 17 <t>PCDD/Fs</t> and Σ 19 <t>PCBs</t> in agricultural-grade (n = 10) and analytical-grade (n = 4) CuSO 4 . The box upper and under lines represent the 25th and 75th percentiles, respectively, and the three horizontal bars represent the 5th, 50th, and 95th percentiles. * represent the 1% and 99%, □ represent mean values.
    Pcb, supplied by Frontier Scientific, used in various techniques. Bioz Stars score: 90/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (a) Total Q factors of the functional microsphere versus the self-assembled polymer layer thickness (t). The experimental data are shown as triangles (for PAH/PB) and dots (for PAH/PCBS). The theoretical fittings are performed using 1/ Q = 1/ Q coupling + A × t α , where Q coupling , A , and α are fitting constants. (b) The relationship between film thickness t and Q film . The fitted values are given by1/ Q film = A × t α . The experimental data are obtained using 1/ Q film = 1/ Q - 1/ Q coupling. .

    Journal: Optics Express

    Article Title: High quality factor silica microspheres functionalized with self-assembled nanomaterials

    doi: 10.1364/OE.21.020601

    Figure Lengend Snippet: (a) Total Q factors of the functional microsphere versus the self-assembled polymer layer thickness (t). The experimental data are shown as triangles (for PAH/PB) and dots (for PAH/PCBS). The theoretical fittings are performed using 1/ Q = 1/ Q coupling + A × t α , where Q coupling , A , and α are fitting constants. (b) The relationship between film thickness t and Q film . The fitted values are given by1/ Q film = A × t α . The experimental data are obtained using 1/ Q film = 1/ Q - 1/ Q coupling. .

    Article Snippet: For this experimental study, PAH, PCBS and PB were purchased from Sigma-Aldrich.

    Techniques: Functional Assay

    (a) Uptake of colchicine in KB3-1 expressing with wild-type human P-glycoprotein (KB3-His 61 , filled circles), mutant P-glycoprotein in which His 61 was replaced with phenylalanine (KB3-Phe 61 , open squares) and serine (KB3-Ser 61 , filled squares), and transfected with transfection vector (KB3-Vec, open circles). (b) The ratios between the accumulations of colchicine with and without cyclosporine A (CYA) and (c) PCB-126 (with/without CYA or PCB-126) in KB3-Vec (open columns), KB3-His 61 (cross-hatched columns), KB3-Phe 61 (hatched columns), and KB3-Ser 61 (filled columns). ∗ , P

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: 3,3?,4,4?,5-Pentachlorobiphenyl Inhibits Drug Efflux Through P-Glycoprotein in KB-3 Cells Expressing Mutant Human P-Glycoprotein

    doi: 10.1155/S1110724304308028

    Figure Lengend Snippet: (a) Uptake of colchicine in KB3-1 expressing with wild-type human P-glycoprotein (KB3-His 61 , filled circles), mutant P-glycoprotein in which His 61 was replaced with phenylalanine (KB3-Phe 61 , open squares) and serine (KB3-Ser 61 , filled squares), and transfected with transfection vector (KB3-Vec, open circles). (b) The ratios between the accumulations of colchicine with and without cyclosporine A (CYA) and (c) PCB-126 (with/without CYA or PCB-126) in KB3-Vec (open columns), KB3-His 61 (cross-hatched columns), KB3-Phe 61 (hatched columns), and KB3-Ser 61 (filled columns). ∗ , P

    Article Snippet: Figures and show the effects of cyclosporine A (b) and PCB-126 (c) on the accumulations of vinblastine after a 3-hours incubation in KB3-Vec and KB3-MDR1s; the ratios between the accumulations with and without cyclosporine A or PCB-126 in each cell group were compared.

    Techniques: Expressing, Mutagenesis, Transfection, Plasmid Preparation

    (a) Uptake of vinblastine in KB3-1 expressing with wild-type human P-glycoprotein (KB3-His 61 , filled circles), mutant P-glycoprotein in which His 61 was replaced with phenylalanine (KB3-Phe 61 , open squares) and serine (KB3-Ser 61 , filled squares), and transfected with transfection vector (KB3-Vec, open circles). (b) The ratios between the accumulations of vinblastine with and without cyclosporine A (CYA) and (c) PCB-126 (with/without CYA or PCB-126) in KB3-Vec (open columns), KB3-His 61 (cross-hatched columns), KB3-Phe 61 (hatched columns), and KB3-Ser 61 (filled columns). ∗ , P

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: 3,3?,4,4?,5-Pentachlorobiphenyl Inhibits Drug Efflux Through P-Glycoprotein in KB-3 Cells Expressing Mutant Human P-Glycoprotein

    doi: 10.1155/S1110724304308028

    Figure Lengend Snippet: (a) Uptake of vinblastine in KB3-1 expressing with wild-type human P-glycoprotein (KB3-His 61 , filled circles), mutant P-glycoprotein in which His 61 was replaced with phenylalanine (KB3-Phe 61 , open squares) and serine (KB3-Ser 61 , filled squares), and transfected with transfection vector (KB3-Vec, open circles). (b) The ratios between the accumulations of vinblastine with and without cyclosporine A (CYA) and (c) PCB-126 (with/without CYA or PCB-126) in KB3-Vec (open columns), KB3-His 61 (cross-hatched columns), KB3-Phe 61 (hatched columns), and KB3-Ser 61 (filled columns). ∗ , P

    Article Snippet: Figures and show the effects of cyclosporine A (b) and PCB-126 (c) on the accumulations of vinblastine after a 3-hours incubation in KB3-Vec and KB3-MDR1s; the ratios between the accumulations with and without cyclosporine A or PCB-126 in each cell group were compared.

    Techniques: Expressing, Mutagenesis, Transfection, Plasmid Preparation

    Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of PCB congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of PCBs released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p

    Journal: PLoS ONE

    Article Title: PCB-153 Shows Different Dynamics of Mobilisation from Differentiated Rat Adipocytes during Lipolysis in Comparison with PCB-28 and PCB-118

    doi: 10.1371/journal.pone.0106495

    Figure Lengend Snippet: Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of PCB congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of PCBs released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p

    Article Snippet: The PCBs were added to the culture medium as ethanolic solution and four conditions of PCB contamination were tested: (i ) 2,4,4′-trichlorobiphenyl (PCB-28); (ii ) 2,3′,4,4′,5-pentachlorobiphenyl (PCB-118); (iii ) 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB-153); (iv ) an equimolar mixture of three PCB congeners (PCB-28, -118 and -153), also called cocktail of PCBs.

    Techniques:

    Presence of other congeners did not influence the dynamic of PCB mobilisation. At day 11, differentiated rat adipocytes, which were previously contaminated with either individual PCB congeners or with a cocktail of PCBs, underwent a lipolytic process. The cellular levels of PCBs before the lipolytic process were quantified and set at 100%. During 12-hour period of lipolysis, the contents of PCB congeners within adipocytes and in the extracellular medium were assessed every 3 hours. The results for PCB-28 (A), PCB-118 (B) and PCB-153 (C) were expressed by the percentage of initial amounts of each congener. Within a condition of contamination, proportions of one PCB congener in the medium were obtained by adding the quantities released during the periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). At each given time of lipolytic treatment, no differences were noted between the proportions of each PCB (i.e. PCB-28, -118 and -153) in both conditions of contamination (i.e. congeners alone or in cocktail), either in the cells or in the lipolytic medium (0.122

    Journal: PLoS ONE

    Article Title: PCB-153 Shows Different Dynamics of Mobilisation from Differentiated Rat Adipocytes during Lipolysis in Comparison with PCB-28 and PCB-118

    doi: 10.1371/journal.pone.0106495

    Figure Lengend Snippet: Presence of other congeners did not influence the dynamic of PCB mobilisation. At day 11, differentiated rat adipocytes, which were previously contaminated with either individual PCB congeners or with a cocktail of PCBs, underwent a lipolytic process. The cellular levels of PCBs before the lipolytic process were quantified and set at 100%. During 12-hour period of lipolysis, the contents of PCB congeners within adipocytes and in the extracellular medium were assessed every 3 hours. The results for PCB-28 (A), PCB-118 (B) and PCB-153 (C) were expressed by the percentage of initial amounts of each congener. Within a condition of contamination, proportions of one PCB congener in the medium were obtained by adding the quantities released during the periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). At each given time of lipolytic treatment, no differences were noted between the proportions of each PCB (i.e. PCB-28, -118 and -153) in both conditions of contamination (i.e. congeners alone or in cocktail), either in the cells or in the lipolytic medium (0.122

    Article Snippet: The PCBs were added to the culture medium as ethanolic solution and four conditions of PCB contamination were tested: (i ) 2,4,4′-trichlorobiphenyl (PCB-28); (ii ) 2,3′,4,4′,5-pentachlorobiphenyl (PCB-118); (iii ) 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB-153); (iv ) an equimolar mixture of three PCB congeners (PCB-28, -118 and -153), also called cocktail of PCBs.

    Techniques:

    Lipolytic treatment decreased cellular neutral lipids and increased extracellular fatty acids and glycerol. At day 11, differentiated rat adipocytes, which were previously contaminated with PCBs, were incubated with a lipolytic medium supplemented with 1 µM isoproterenol. We renewed the lipolytic medium every 3 hours for 12 hours. Cellular neutral lipids (corresponding to µmol of fatty acids in cellular neutral lipids) were expressed per mg of total cell protein (A). A significant decrease of cellular lipid contents was noted throughout the lipolytic process for the conditions with PCB-28 ( p = 0.005) and the cocktail of PCBs ( p = 0.029). The decrease was slighter for the conditions with PCB-118 ( p = 0.298) and PCB-153 ( p = 0.097). Total extracellular free fatty acids (B) and total extracellular glycerol (C) were expressed per ml of medium. Quantities of total free fatty acids and glycerol in the medium were obtained by adding the quantities released during the periods of 3 hours (e.g. total free fatty acids at 6 hours correspond to the sum of total free fatty acids released between 0 and 3 hours and between 3 and 6 hours). A significant increase of total free FAs and total glycerol was observed over the 12-hour lipolytic treatment ( p

    Journal: PLoS ONE

    Article Title: PCB-153 Shows Different Dynamics of Mobilisation from Differentiated Rat Adipocytes during Lipolysis in Comparison with PCB-28 and PCB-118

    doi: 10.1371/journal.pone.0106495

    Figure Lengend Snippet: Lipolytic treatment decreased cellular neutral lipids and increased extracellular fatty acids and glycerol. At day 11, differentiated rat adipocytes, which were previously contaminated with PCBs, were incubated with a lipolytic medium supplemented with 1 µM isoproterenol. We renewed the lipolytic medium every 3 hours for 12 hours. Cellular neutral lipids (corresponding to µmol of fatty acids in cellular neutral lipids) were expressed per mg of total cell protein (A). A significant decrease of cellular lipid contents was noted throughout the lipolytic process for the conditions with PCB-28 ( p = 0.005) and the cocktail of PCBs ( p = 0.029). The decrease was slighter for the conditions with PCB-118 ( p = 0.298) and PCB-153 ( p = 0.097). Total extracellular free fatty acids (B) and total extracellular glycerol (C) were expressed per ml of medium. Quantities of total free fatty acids and glycerol in the medium were obtained by adding the quantities released during the periods of 3 hours (e.g. total free fatty acids at 6 hours correspond to the sum of total free fatty acids released between 0 and 3 hours and between 3 and 6 hours). A significant increase of total free FAs and total glycerol was observed over the 12-hour lipolytic treatment ( p

    Article Snippet: The PCBs were added to the culture medium as ethanolic solution and four conditions of PCB contamination were tested: (i ) 2,4,4′-trichlorobiphenyl (PCB-28); (ii ) 2,3′,4,4′,5-pentachlorobiphenyl (PCB-118); (iii ) 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB-153); (iv ) an equimolar mixture of three PCB congeners (PCB-28, -118 and -153), also called cocktail of PCBs.

    Techniques: Incubation

    The percentage of initial molar concentrations for total PCB (orange), PCB 52 (green), PCB 77 (blue), and PCB 153(purple) after 12 weeks and 24 weeks incubation in unplanted soil (UP), switchgrass treated soil (SG), unplanted with bioaugmentaion soil

    Journal: Ecological engineering

    Article Title: Enhanced Polychlorinated Biphenyl Removal in a Switchgrass Rhizosphere by Bioaugmentation with Burkholderia xenovorans LB400

    doi: 10.1016/j.ecoleng.2014.07.046

    Figure Lengend Snippet: The percentage of initial molar concentrations for total PCB (orange), PCB 52 (green), PCB 77 (blue), and PCB 153(purple) after 12 weeks and 24 weeks incubation in unplanted soil (UP), switchgrass treated soil (SG), unplanted with bioaugmentaion soil

    Article Snippet: The soil was spiked with a mixture of PCB 52, PCB 77 and PCB 153 (99% pure) (Accustandard Inc., New Haven, CT) at the concentration of 500 ng g−1 each.

    Techniques: Incubation

    PCB 153 causes NF-κB mediated intestinal inflammation and increases gut permeability Mice were orally exposed once per day to PCB 153 (300μmol/kg) or vehicle for 2 days, following IP injections of the NF-κB inhibitor PDTC or control (PBS). (A.) IECs were isolated, RNA was extracted and RT-qPCR run for IL-6 and (B.) TNFα. N=5 mice per group. Two-way AVOVA with Tukey’s multiple comparison test. **P

    Journal: Toxicology and applied pharmacology

    Article Title: Intestinal exposure to PCB 153 induces inflammation via the ATM/NEMO pathway

    doi: 10.1016/j.taap.2017.11.027

    Figure Lengend Snippet: PCB 153 causes NF-κB mediated intestinal inflammation and increases gut permeability Mice were orally exposed once per day to PCB 153 (300μmol/kg) or vehicle for 2 days, following IP injections of the NF-κB inhibitor PDTC or control (PBS). (A.) IECs were isolated, RNA was extracted and RT-qPCR run for IL-6 and (B.) TNFα. N=5 mice per group. Two-way AVOVA with Tukey’s multiple comparison test. **P

    Article Snippet: PCB 153 (2,2´,4,4´,5,5´-hexachlorobiphenyl) was purchased from Sigma-Aldrich (St. Louis, MO) and dissolved in dimethyl sulfoxide (DMSO, EMD Millipore Corp., Billerica, MA) to make the stock solution (10mM).

    Techniques: Permeability, Mouse Assay, Isolation, Quantitative RT-PCR

    PCB 153 activates NF-κB via the genotoxic response pathway and ATM/NEMO (A.) SW480 cells were transfected with a NF-κB reporter plasmid and were pretreated for 2hrs with 100 ng/mL of C. difficile Toxin B (Tox) to specifically inhibit genotoxic activation of NF-κB while allowing LPS-induced activation. They were then exposed to vehicle (DMSO), PCB 153 (10μM), etoposide (10μM), or LPS (50 ng/mL) for 3 hrs. (N > 4) Two-way AVOVA with Tukey’s multiple comparison test, ns = not significant. *P

    Journal: Toxicology and applied pharmacology

    Article Title: Intestinal exposure to PCB 153 induces inflammation via the ATM/NEMO pathway

    doi: 10.1016/j.taap.2017.11.027

    Figure Lengend Snippet: PCB 153 activates NF-κB via the genotoxic response pathway and ATM/NEMO (A.) SW480 cells were transfected with a NF-κB reporter plasmid and were pretreated for 2hrs with 100 ng/mL of C. difficile Toxin B (Tox) to specifically inhibit genotoxic activation of NF-κB while allowing LPS-induced activation. They were then exposed to vehicle (DMSO), PCB 153 (10μM), etoposide (10μM), or LPS (50 ng/mL) for 3 hrs. (N > 4) Two-way AVOVA with Tukey’s multiple comparison test, ns = not significant. *P

    Article Snippet: PCB 153 (2,2´,4,4´,5,5´-hexachlorobiphenyl) was purchased from Sigma-Aldrich (St. Louis, MO) and dissolved in dimethyl sulfoxide (DMSO, EMD Millipore Corp., Billerica, MA) to make the stock solution (10mM).

    Techniques: Transfection, Plasmid Preparation, Activation Assay

    ATM is required for PCB 153-induced inflammation (A.)  SW480 cells were pretreated for 1hr with 10μM of KU-55833. They were then exposed to etoposide (10μM) and PCB153 (100μM) or vehicle (DMSO) for 3 hrs. RNA was collected and RT-qPCR run for IL-6 and  (B.)  TNFα. (N=4) Two-way AVOVA with Tukey’s multiple comparison test. *P

    Journal: Toxicology and applied pharmacology

    Article Title: Intestinal exposure to PCB 153 induces inflammation via the ATM/NEMO pathway

    doi: 10.1016/j.taap.2017.11.027

    Figure Lengend Snippet: ATM is required for PCB 153-induced inflammation (A.) SW480 cells were pretreated for 1hr with 10μM of KU-55833. They were then exposed to etoposide (10μM) and PCB153 (100μM) or vehicle (DMSO) for 3 hrs. RNA was collected and RT-qPCR run for IL-6 and (B.) TNFα. (N=4) Two-way AVOVA with Tukey’s multiple comparison test. *P

    Article Snippet: PCB 153 (2,2´,4,4´,5,5´-hexachlorobiphenyl) was purchased from Sigma-Aldrich (St. Louis, MO) and dissolved in dimethyl sulfoxide (DMSO, EMD Millipore Corp., Billerica, MA) to make the stock solution (10mM).

    Techniques: Quantitative RT-PCR

    PCB 153 causes DNA damage (A.) IECs were isolated from mice after 2 days of exposure to 300μmol/kg of PCB 153 or vehicle via oral gavage. Protein was extracted and westerns run for γ-H2A.X. (B.) Densitometry quantification of 3A. N=4–5 mice per group. Unpaired, two-tailed T test. **P

    Journal: Toxicology and applied pharmacology

    Article Title: Intestinal exposure to PCB 153 induces inflammation via the ATM/NEMO pathway

    doi: 10.1016/j.taap.2017.11.027

    Figure Lengend Snippet: PCB 153 causes DNA damage (A.) IECs were isolated from mice after 2 days of exposure to 300μmol/kg of PCB 153 or vehicle via oral gavage. Protein was extracted and westerns run for γ-H2A.X. (B.) Densitometry quantification of 3A. N=4–5 mice per group. Unpaired, two-tailed T test. **P

    Article Snippet: PCB 153 (2,2´,4,4´,5,5´-hexachlorobiphenyl) was purchased from Sigma-Aldrich (St. Louis, MO) and dissolved in dimethyl sulfoxide (DMSO, EMD Millipore Corp., Billerica, MA) to make the stock solution (10mM).

    Techniques: Isolation, Mouse Assay, Two Tailed Test

    In vitro activation of NF-κB by PCB 153 is ameliorated by free radical scavenger (A) SW480 cells were transfected with a NF-κB reporter plasmid. Cells were pretreated for 1 hr with 5mM N-Acetyl-Cysteine (NAC), a free radical scavenger, or control (PBS). Cells were subsequently exposed to LPS, PCB153, or vehicle (DMSO) for 3 hrs. (N=5) Two-way AVOVA with Tukey’s multiple comparison test. *P

    Journal: Toxicology and applied pharmacology

    Article Title: Intestinal exposure to PCB 153 induces inflammation via the ATM/NEMO pathway

    doi: 10.1016/j.taap.2017.11.027

    Figure Lengend Snippet: In vitro activation of NF-κB by PCB 153 is ameliorated by free radical scavenger (A) SW480 cells were transfected with a NF-κB reporter plasmid. Cells were pretreated for 1 hr with 5mM N-Acetyl-Cysteine (NAC), a free radical scavenger, or control (PBS). Cells were subsequently exposed to LPS, PCB153, or vehicle (DMSO) for 3 hrs. (N=5) Two-way AVOVA with Tukey’s multiple comparison test. *P

    Article Snippet: PCB 153 (2,2´,4,4´,5,5´-hexachlorobiphenyl) was purchased from Sigma-Aldrich (St. Louis, MO) and dissolved in dimethyl sulfoxide (DMSO, EMD Millipore Corp., Billerica, MA) to make the stock solution (10mM).

    Techniques: In Vitro, Activation Assay, Transfection, Plasmid Preparation

    Concentrations of Σ 17 PCDD/Fs and Σ 19 PCBs in agricultural-grade (n = 10) and analytical-grade (n = 4) CuSO 4 . The box upper and under lines represent the 25th and 75th percentiles, respectively, and the three horizontal bars represent the 5th, 50th, and 95th percentiles. * represent the 1% and 99%, □ represent mean values.

    Journal: PLoS ONE

    Article Title: Assessment of the Polychlorinated Biphenyl (PCB) Occurrence in Copper Sulfates and the Influential Role of PCB Levels on Grapes

    doi: 10.1371/journal.pone.0144896

    Figure Lengend Snippet: Concentrations of Σ 17 PCDD/Fs and Σ 19 PCBs in agricultural-grade (n = 10) and analytical-grade (n = 4) CuSO 4 . The box upper and under lines represent the 25th and 75th percentiles, respectively, and the three horizontal bars represent the 5th, 50th, and 95th percentiles. * represent the 1% and 99%, □ represent mean values.

    Article Snippet: Reagents and Materials 13 C-labeled surrogate standards for PCDD/Fs (EPA 1613-LCS) and PCBs (EPA 68A-LCS) and 13 C-labeled injection standards for PCDD/Fs (EPA 1613-IS) and PCBs (EPA 68A-IS) were all purchased from Wellington Laboratories (Guelph, Canada).

    Techniques: