Journal: PLoS ONE
Article Title: PCB-153 Shows Different Dynamics of Mobilisation from Differentiated Rat Adipocytes during Lipolysis in Comparison with PCB-28 and PCB-118
Figure Lengend Snippet: Presence of other congeners did not influence the dynamic of PCB mobilisation. At day 11, differentiated rat adipocytes, which were previously contaminated with either individual PCB congeners or with a cocktail of PCBs, underwent a lipolytic process. The cellular levels of PCBs before the lipolytic process were quantified and set at 100%. During 12-hour period of lipolysis, the contents of PCB congeners within adipocytes and in the extracellular medium were assessed every 3 hours. The results for PCB-28 (A), PCB-118 (B) and PCB-153 (C) were expressed by the percentage of initial amounts of each congener. Within a condition of contamination, proportions of one PCB congener in the medium were obtained by adding the quantities released during the periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). At each given time of lipolytic treatment, no differences were noted between the proportions of each PCB (i.e. PCB-28, -118 and -153) in both conditions of contamination (i.e. congeners alone or in cocktail), either in the cells or in the lipolytic medium (0.122
Article Snippet: The PCBs were added to the culture medium as ethanolic solution and four conditions of PCB contamination were tested: (i ) 2,4,4′-trichlorobiphenyl (PCB-28); (ii ) 2,3′,4,4′,5-pentachlorobiphenyl (PCB-118); (iii ) 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB-153); (iv ) an equimolar mixture of three PCB congeners (PCB-28, -118 and -153), also called cocktail of PCBs.