Journal: Chemical research in toxicology

Article Title: Toxicokinetics of Chiral PCB 136 and its Hydroxylated Metabolites in Mice with a Liver-Specific Deletion of Cytochrome P450 Reductase

doi: 10.1021/acs.chemrestox.8b00389

Figure Lengend Snippet: The molar percentage of PCB 136 and the sum of hydroxylated metabolites, ΣHO-PCBs, changed over exposure time in whole blood from (A) male KO (M-KO), (B) male wildtype (M-WT), (C) female KO (F-KO), (D) female wildtype (F-WT) mice. The dotted lines are trendlines added to visualize the change of the molar percentage of PCB 136 vs. ΣHO-PCBs in mouse blood over time. The molar percentages of PCBs and OH-PCBs were calculated from the sum of the PCB and OH-PCB levels in the whole blood from each animal and subsequently averaged for all animals within the exposure group.

Article Snippet: Diazomethane for the derivatization of hydroxylated PCBs to methoxylated PCBs was synthesized from N -methyl- N -nitroso- p -tolulenesulfonamide (Diazald) using an Aldrich mini Diazald apparatus (Milwaukee, WI, USA).

Techniques: Mouse Assay

Journal: Journal of Biomedicine and Biotechnology

Article Title: 3,3?,4,4?,5-Pentachlorobiphenyl Inhibits Drug Efflux Through P-Glycoprotein in KB-3 Cells Expressing Mutant Human P-Glycoprotein

doi: 10.1155/S1110724304308028

Figure Lengend Snippet: (a) Uptake of colchicine in KB3-1 expressing with wild-type human P-glycoprotein (KB3-His 61 , filled circles), mutant P-glycoprotein in which His 61 was replaced with phenylalanine (KB3-Phe 61 , open squares) and serine (KB3-Ser 61 , filled squares), and transfected with transfection vector (KB3-Vec, open circles). (b) The ratios between the accumulations of colchicine with and without cyclosporine A (CYA) and (c) PCB-126 (with/without CYA or PCB-126) in KB3-Vec (open columns), KB3-His 61 (cross-hatched columns), KB3-Phe 61 (hatched columns), and KB3-Ser 61 (filled columns). ∗ , P

Article Snippet: Figures and show the effects of cyclosporine A (b) and PCB-126 (c) on the accumulations of vinblastine after a 3-hours incubation in KB3-Vec and KB3-MDR1s; the ratios between the accumulations with and without cyclosporine A or PCB-126 in each cell group were compared.

Techniques: Expressing, Mutagenesis, Transfection, Plasmid Preparation

Journal: Journal of Biomedicine and Biotechnology

Article Title: 3,3?,4,4?,5-Pentachlorobiphenyl Inhibits Drug Efflux Through P-Glycoprotein in KB-3 Cells Expressing Mutant Human P-Glycoprotein

doi: 10.1155/S1110724304308028

Figure Lengend Snippet: (a) Uptake of vinblastine in KB3-1 expressing with wild-type human P-glycoprotein (KB3-His 61 , filled circles), mutant P-glycoprotein in which His 61 was replaced with phenylalanine (KB3-Phe 61 , open squares) and serine (KB3-Ser 61 , filled squares), and transfected with transfection vector (KB3-Vec, open circles). (b) The ratios between the accumulations of vinblastine with and without cyclosporine A (CYA) and (c) PCB-126 (with/without CYA or PCB-126) in KB3-Vec (open columns), KB3-His 61 (cross-hatched columns), KB3-Phe 61 (hatched columns), and KB3-Ser 61 (filled columns). ∗ , P

Article Snippet: Figures and show the effects of cyclosporine A (b) and PCB-126 (c) on the accumulations of vinblastine after a 3-hours incubation in KB3-Vec and KB3-MDR1s; the ratios between the accumulations with and without cyclosporine A or PCB-126 in each cell group were compared.

Techniques: Expressing, Mutagenesis, Transfection, Plasmid Preparation

Journal: PLoS ONE

Article Title: PCB-153 Shows Different Dynamics of Mobilisation from Differentiated Rat Adipocytes during Lipolysis in Comparison with PCB-28 and PCB-118

doi: 10.1371/journal.pone.0106495

Figure Lengend Snippet: Lipolytic treatment decontaminated the adipocytes, inducing an accumulation of PCB congeners in the extracellular medium. At day 11, contaminated adipocytes underwent a lipolytic process. The PCB contents as well as the proportions of PCBs released in the extracellular medium were assessed every 3 hours. For each PCB congener, all results were expressed in percentage of the amounts initially present in the cells. Proportions of one PCB congener in the medium were obtained by adding the amounts released during periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). An important drop of cell PCB proportions was observed during the first 6 hours of lipolysis ( p

Article Snippet: The PCBs were added to the culture medium as ethanolic solution and four conditions of PCB contamination were tested: (i ) 2,4,4′-trichlorobiphenyl (PCB-28); (ii ) 2,3′,4,4′,5-pentachlorobiphenyl (PCB-118); (iii ) 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB-153); (iv ) an equimolar mixture of three PCB congeners (PCB-28, -118 and -153), also called cocktail of PCBs.

Techniques:

Journal: PLoS ONE

Article Title: PCB-153 Shows Different Dynamics of Mobilisation from Differentiated Rat Adipocytes during Lipolysis in Comparison with PCB-28 and PCB-118

doi: 10.1371/journal.pone.0106495

Figure Lengend Snippet: Presence of other congeners did not influence the dynamic of PCB mobilisation. At day 11, differentiated rat adipocytes, which were previously contaminated with either individual PCB congeners or with a cocktail of PCBs, underwent a lipolytic process. The cellular levels of PCBs before the lipolytic process were quantified and set at 100%. During 12-hour period of lipolysis, the contents of PCB congeners within adipocytes and in the extracellular medium were assessed every 3 hours. The results for PCB-28 (A), PCB-118 (B) and PCB-153 (C) were expressed by the percentage of initial amounts of each congener. Within a condition of contamination, proportions of one PCB congener in the medium were obtained by adding the quantities released during the periods of 3 hours (e.g. proportion of one congener at 6 hours corresponds to the sum of this congener released between 0 and 3 hours and between 3 and 6 hours). At each given time of lipolytic treatment, no differences were noted between the proportions of each PCB (i.e. PCB-28, -118 and -153) in both conditions of contamination (i.e. congeners alone or in cocktail), either in the cells or in the lipolytic medium (0.122

Article Snippet: The PCBs were added to the culture medium as ethanolic solution and four conditions of PCB contamination were tested: (i ) 2,4,4′-trichlorobiphenyl (PCB-28); (ii ) 2,3′,4,4′,5-pentachlorobiphenyl (PCB-118); (iii ) 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB-153); (iv ) an equimolar mixture of three PCB congeners (PCB-28, -118 and -153), also called cocktail of PCBs.

Techniques:

Journal: PLoS ONE

Article Title: PCB-153 Shows Different Dynamics of Mobilisation from Differentiated Rat Adipocytes during Lipolysis in Comparison with PCB-28 and PCB-118

doi: 10.1371/journal.pone.0106495

Figure Lengend Snippet: Lipolytic treatment decreased cellular neutral lipids and increased extracellular fatty acids and glycerol. At day 11, differentiated rat adipocytes, which were previously contaminated with PCBs, were incubated with a lipolytic medium supplemented with 1 µM isoproterenol. We renewed the lipolytic medium every 3 hours for 12 hours. Cellular neutral lipids (corresponding to µmol of fatty acids in cellular neutral lipids) were expressed per mg of total cell protein (A). A significant decrease of cellular lipid contents was noted throughout the lipolytic process for the conditions with PCB-28 ( p = 0.005) and the cocktail of PCBs ( p = 0.029). The decrease was slighter for the conditions with PCB-118 ( p = 0.298) and PCB-153 ( p = 0.097). Total extracellular free fatty acids (B) and total extracellular glycerol (C) were expressed per ml of medium. Quantities of total free fatty acids and glycerol in the medium were obtained by adding the quantities released during the periods of 3 hours (e.g. total free fatty acids at 6 hours correspond to the sum of total free fatty acids released between 0 and 3 hours and between 3 and 6 hours). A significant increase of total free FAs and total glycerol was observed over the 12-hour lipolytic treatment ( p

Article Snippet: The PCBs were added to the culture medium as ethanolic solution and four conditions of PCB contamination were tested: (i ) 2,4,4′-trichlorobiphenyl (PCB-28); (ii ) 2,3′,4,4′,5-pentachlorobiphenyl (PCB-118); (iii ) 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB-153); (iv ) an equimolar mixture of three PCB congeners (PCB-28, -118 and -153), also called cocktail of PCBs.

Techniques: Incubation

Journal: Environmental science and pollution research international

Article Title: Exercise Protects against PCB-Induced Inflammation and Associated Cardiovascular Risk Factors

doi: 10.1007/s11356-014-4062-6

Figure Lengend Snippet: Exercise attenuates PCB 77-impaired glucose intolerance. A) Blood glucose concentrations. B) Total area under the curve (AUC) calculates the area below the observed concentrations. Data present mean ± SEM (n=8). *Significantly different compared

Article Snippet: PCB 77 was purchased from Accustandard Inc. (New Haven, CT).

Techniques:

Journal: Environmental science and pollution research international

Article Title: Exercise Protects against PCB-Induced Inflammation and Associated Cardiovascular Risk Factors

doi: 10.1007/s11356-014-4062-6

Figure Lengend Snippet: Exercise prevents upregulation of proinflammatory cytokines induced by PCB 77 exposure. Plasma samples were analyzed for IL-6, MCP-1, and M-CSF levels. Data represent the mean± SEM (n=5). * Significantly different compared to vehicle control,

Article Snippet: PCB 77 was purchased from Accustandard Inc. (New Haven, CT).

Techniques:

Journal: Environmental science and pollution research international

Article Title: Exercise Protects against PCB-Induced Inflammation and Associated Cardiovascular Risk Factors

doi: 10.1007/s11356-014-4062-6

Figure Lengend Snippet: Exercise modulates PCB 77-induced oxidative stress. Urine F 2 -isoprostane levels were measured by HPLC/MS MS. All values were normalized to urine creatinine levels and for IS recovery. Data are represented as mean ± SEM (n=5). * Significantly different

Article Snippet: PCB 77 was purchased from Accustandard Inc. (New Haven, CT).

Techniques: High Performance Liquid Chromatography, Mass Spectrometry

Journal: PLoS ONE

Article Title: Assessment of the Polychlorinated Biphenyl (PCB) Occurrence in Copper Sulfates and the Influential Role of PCB Levels on Grapes

doi: 10.1371/journal.pone.0144896

Figure Lengend Snippet: Concentrations of Σ 17 PCDD/Fs and Σ 19 PCBs in agricultural-grade (n = 10) and analytical-grade (n = 4) CuSO 4 . The box upper and under lines represent the 25th and 75th percentiles, respectively, and the three horizontal bars represent the 5th, 50th, and 95th percentiles. * represent the 1% and 99%, □ represent mean values.

Article Snippet: Reagents and Materials 13 C-labeled surrogate standards for PCDD/Fs (EPA 1613-LCS) and PCBs (EPA 68A-LCS) and 13 C-labeled injection standards for PCDD/Fs (EPA 1613-IS) and PCBs (EPA 68A-IS) were all purchased from Wellington Laboratories (Guelph, Canada).

Techniques: