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  • 99
    Thermo Fisher phosphate buffered saline pbs pbs
    Specificity of improved <t>scFv</t> variants. Binding of the selected scFv candidates was tested in an ELISA against a panel of human chemokines. NusA fusion chemokine was coated at 20 µg/ml and 5 µg/ml variant scFv in 1% <t>milk-PBS</t> buffer was incubated for 1 hour at room temperature. Coating was controlled using specific mAb for each chemokine and NusA protein was also added to the assay as negative control (data not shown). Results are expressed as mean ± S.D. of duplicates of two representative experiments.
    Phosphate Buffered Saline Pbs Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad buffer phosphate buffered saline pbs
    Membrane association of LidA after translocation into host cells. (A) L. pneumophila (WT) and an isogenic dotA mutant ( dotA ), both expressing <t>GFP,</t> were grown to postexponential phase and resuspended in <t>PBS</t> containing either 1% Triton X-100, 1% CHAPS,
    Buffer Phosphate Buffered Saline Pbs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc phosphate buffered saline pbs buffer
    <t>Metformin</t> inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or <t>PBS</t> by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,
    Phosphate Buffered Saline Pbs Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs buffer/product/Cell Signaling Technology Inc
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    Boster Bio phosphate buffered saline pbs
    <t>Metformin</t> inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or <t>PBS</t> by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,
    Phosphate Buffered Saline Pbs, supplied by Boster Bio, used in various techniques. Bioz Stars score: 99/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa phosphate buffered saline pbs
    <t>Metformin</t> inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or <t>PBS</t> by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,
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    Specificity of improved scFv variants. Binding of the selected scFv candidates was tested in an ELISA against a panel of human chemokines. NusA fusion chemokine was coated at 20 µg/ml and 5 µg/ml variant scFv in 1% milk-PBS buffer was incubated for 1 hour at room temperature. Coating was controlled using specific mAb for each chemokine and NusA protein was also added to the assay as negative control (data not shown). Results are expressed as mean ± S.D. of duplicates of two representative experiments.

    Journal: mAbs

    Article Title: Specificity tuning of antibody fragments to neutralize two human chemokines with a single agent

    doi:

    Figure Lengend Snippet: Specificity of improved scFv variants. Binding of the selected scFv candidates was tested in an ELISA against a panel of human chemokines. NusA fusion chemokine was coated at 20 µg/ml and 5 µg/ml variant scFv in 1% milk-PBS buffer was incubated for 1 hour at room temperature. Coating was controlled using specific mAb for each chemokine and NusA protein was also added to the assay as negative control (data not shown). Results are expressed as mean ± S.D. of duplicates of two representative experiments.

    Article Snippet: scFv phage libraries (1012 Pfu) were blocked with phosphate buffered saline (PBS) containing 3% (w/v) skimmed milk and then deselected on streptavidin magnetic beads (Dynal M-280).

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Variant Assay, Incubation, Negative Control

    Dose-response of the variants for hCXCL9 and hCXCL10 binding. ELISA were performed using different concentrations of purified scFv against hCXCL10 (A) or hCXCL9 (B). Chemokine target was coated either at 2 µg/ml for NusA-hCXCL10 or 5 µg/ml for NusA-hCXCL9 fusion protein. Serial 3-fold dilutions of scFv were performed in 1% milk-PBS buffer and scFv was incubated for one hour at room temperature. Results are expressed as mean ± S.D. of duplicates.

    Journal: mAbs

    Article Title: Specificity tuning of antibody fragments to neutralize two human chemokines with a single agent

    doi:

    Figure Lengend Snippet: Dose-response of the variants for hCXCL9 and hCXCL10 binding. ELISA were performed using different concentrations of purified scFv against hCXCL10 (A) or hCXCL9 (B). Chemokine target was coated either at 2 µg/ml for NusA-hCXCL10 or 5 µg/ml for NusA-hCXCL9 fusion protein. Serial 3-fold dilutions of scFv were performed in 1% milk-PBS buffer and scFv was incubated for one hour at room temperature. Results are expressed as mean ± S.D. of duplicates.

    Article Snippet: scFv phage libraries (1012 Pfu) were blocked with phosphate buffered saline (PBS) containing 3% (w/v) skimmed milk and then deselected on streptavidin magnetic beads (Dynal M-280).

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Purification, Incubation

    Membrane association of LidA after translocation into host cells. (A) L. pneumophila (WT) and an isogenic dotA mutant ( dotA ), both expressing GFP, were grown to postexponential phase and resuspended in PBS containing either 1% Triton X-100, 1% CHAPS,

    Journal:

    Article Title: LidA, a Translocated Substrate of the Legionella pneumophila Type IV Secretion System, Interferes with the Early Secretory Pathway

    doi: 10.1128/IAI.73.7.4370-4380.2005

    Figure Lengend Snippet: Membrane association of LidA after translocation into host cells. (A) L. pneumophila (WT) and an isogenic dotA mutant ( dotA ), both expressing GFP, were grown to postexponential phase and resuspended in PBS containing either 1% Triton X-100, 1% CHAPS,

    Article Snippet: Sixty microliters of postexponential L. pneumophila expressing green fluorescent protein (GFP) was washed once and resuspended in 5 ml of phosphate-buffered saline (PBS) containing either 1% Triton X-100 (Bio-Rad), 1% CHAPS {3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate; Sigma}, 1% octylglucoside (Calbiochem), or 1% purified digitonin (Calbiochem) and incubated for 20 min at room temperature with intermittent vortexing.

    Techniques: Translocation Assay, Mutagenesis, Expressing

    Enzymatic N -sulfation and chemical acetylation of hexasaccharides. Reagents and conditions: a) 1. AG 50W-X8 resin (pyridinium form); 2. DMSO/H 2 O (95:5), 60% for 23 , 80% for 24 , 72% for 25 ; b) PAPS, NST, MES (50 mM, pH 7.1); c) AcOSu, PBS (pH 7.4), 65% for 29 , 45% for 30 , 100% for 31 .

    Journal: Angewandte Chemie (International ed. in English)

    Article Title: Controlled Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides

    doi: 10.1002/anie.201800387

    Figure Lengend Snippet: Enzymatic N -sulfation and chemical acetylation of hexasaccharides. Reagents and conditions: a) 1. AG 50W-X8 resin (pyridinium form); 2. DMSO/H 2 O (95:5), 60% for 23 , 80% for 24 , 72% for 25 ; b) PAPS, NST, MES (50 mM, pH 7.1); c) AcOSu, PBS (pH 7.4), 65% for 29 , 45% for 30 , 100% for 31 .

    Article Snippet: The compounds 26 – 28 were chemically N-acetylated with N -acetyl succinimide (AcOSu) in PBS buffer to give 29 – 31 , respectively, after purification by BioRad P4 size exclusion column chromatography.

    Techniques: Papanicolaou Stain

    The implementation of size-exclusion chromatography to remove Optiprep remnants from EV samples. ( a ) Optiprep density gradients were loaded with PBS to obtain blank EV-density fractions (F1) and with CCM to obtain EV-containing fractions (F2). EVs were retrieved by pelleting at 100,000 g (P1) or by size-exclusion chromatography (S1). Quantity of Optiprep in S1 and P1 was calculated using DC Protein assay. SEC was performed on F2 and consecutive SEC fractions (S2) were analyzed by NTA and protein analysis for GFP and CD9. ( b ) Comparative graph of NTA from S2 (connected line, marked area = SD) and Optiprep quantification of S1 (three differently colored bars representing three independent gradients, SD indicated). ( c ) Protein analysis of S2 by Western blot analysis for GFP and CD9 quantification by TRIFic CD9 assay. Original immunostaining results are shown in Supplementary Fig. 10 . Abbreviations: PBS: phosphate buffered saline. CCM: concentrated conditioned medium. GFP: green fluorescent protein. EV: extracellular vesicle. WB: Western blot. NTA: Nanoparticle tracking analysis. SEC: size-exclusion chromatography.

    Journal: Scientific Reports

    Article Title: Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research

    doi: 10.1038/s41598-017-02599-y

    Figure Lengend Snippet: The implementation of size-exclusion chromatography to remove Optiprep remnants from EV samples. ( a ) Optiprep density gradients were loaded with PBS to obtain blank EV-density fractions (F1) and with CCM to obtain EV-containing fractions (F2). EVs were retrieved by pelleting at 100,000 g (P1) or by size-exclusion chromatography (S1). Quantity of Optiprep in S1 and P1 was calculated using DC Protein assay. SEC was performed on F2 and consecutive SEC fractions (S2) were analyzed by NTA and protein analysis for GFP and CD9. ( b ) Comparative graph of NTA from S2 (connected line, marked area = SD) and Optiprep quantification of S1 (three differently colored bars representing three independent gradients, SD indicated). ( c ) Protein analysis of S2 by Western blot analysis for GFP and CD9 quantification by TRIFic CD9 assay. Original immunostaining results are shown in Supplementary Fig. 10 . Abbreviations: PBS: phosphate buffered saline. CCM: concentrated conditioned medium. GFP: green fluorescent protein. EV: extracellular vesicle. WB: Western blot. NTA: Nanoparticle tracking analysis. SEC: size-exclusion chromatography.

    Article Snippet: A dilution series of 0 to 10% of Optiprep in PBS was obtained as a standard curve for performing absorbance readings with DC Protein assay (Bio-Rad, Hercules, California, USA), according to manufacturer’s instructions.

    Techniques: Size-exclusion Chromatography, DC Protein Assay, Western Blot, Immunostaining

    Protein secretion augments following TS. Stationary parasites were washed 3 times in PBS and resuspended in serum free medium to final density of ∼2×10 8 /ml. Parasites were incubated at 25°C or 37°C for 2 and 4 h. Following incubation, parasites were removed by centrifugation and the supernatant immediately precipitated with TCA/acetone. Precipitated proteins were run on SDS-PAGE and visualized by silver staining. Clear augmentation of protein release in response TS can be seen.

    Journal: PLoS ONE

    Article Title: Temperature-Induced Protein Secretion by Leishmania mexicana Modulates Macrophage Signalling and Function

    doi: 10.1371/journal.pone.0018724

    Figure Lengend Snippet: Protein secretion augments following TS. Stationary parasites were washed 3 times in PBS and resuspended in serum free medium to final density of ∼2×10 8 /ml. Parasites were incubated at 25°C or 37°C for 2 and 4 h. Following incubation, parasites were removed by centrifugation and the supernatant immediately precipitated with TCA/acetone. Precipitated proteins were run on SDS-PAGE and visualized by silver staining. Clear augmentation of protein release in response TS can be seen.

    Article Snippet: Exoproteome preparation and proteomic analysis Stationary L. mexicana promastigotes were washed 3 times in phosphate buffer saline (PBS) and resuspended at ∼108 parasites/ml in serum free DMEM or phenol red-free RPMI media and incubated for 2–4 h. Culture supernatants were isolated by centrifugation twice at 4000 rpm for 10 min. Proteins in the supernatant were dosed using Quick start Bradford reagent (Biorad), precipitated with 15% trichloroacetic acid (TCA)/acetone or concentrated ∼25-fold using 10KD-cut off centrifugal column (Amicon Ultra).

    Techniques: Incubation, Centrifugation, SDS Page, Silver Staining

    Metformin inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or PBS by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Metformin induces autophagy and G0/G1 phase cell cycle arrest in myeloma by targeting the AMPK/mTORC1 and mTORC2 pathways

    doi: 10.1186/s13046-018-0731-5

    Figure Lengend Snippet: Metformin inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or PBS by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,

    Article Snippet: Reagents Metformin (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in phosphate-buffered saline (PBS) as a stock solution of 1 M. Primary antibodies for specific detection of p-AMPK (Thr172), AMPK, Phospho-Tuberin/TSC2 (Ser1387), p-mTOR (Ser2481), p-mTOR (Ser2448), mTOR, p-p70S6K (Thr389), p70S6K, p-4EBP1 (Thr37/46), 4EBP1, p-AKT (Ser473), and AKT, as well as horseradish peroxidase (HRP)-conjugated anti-rabbit secondary detection antibodies, were purchased from Cell Signaling Technology (Beverly, MA, USA).

    Techniques: Mouse Assay, Immunohistochemistry, Expressing