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  • 95
    New England Biolabs molecular biology grade bsa pbs
    Molecular Biology Grade Bsa Pbs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore pbs
    Pbs, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 34240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    New England Biolabs pmvp pb dest vectors nebuilder hifi dna assembly
    Pmvp Pb Dest Vectors Nebuilder Hifi Dna Assembly, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs cutsmart buffer
    Cutsmart Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2026 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs phi29 dna polymerase
    Phi29 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    New England Biolabs hdac6 antibody
    Changes in <t>HDAC6</t> and TP53BP1 transcript and protein levels in response to hypoxia. ( a ) Expression across introns in HDAC6 at 0, 2, 24 h following a shift to reduced oxygen (1%). Colour represents log 2 fold-change. ( b ) HDAC6 protein levels as determined by western blot ( c ). ( d–f ) TP53BP1 exhibits similar patterns of expression.
    Hdac6 Antibody, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Cell Signaling Technology Inc anti pparγ rabbit antibody
    The <t>PPARγ</t> acts as an effector element in determining the reduction of cell number induced by the activation of PI(4,5)P2/PLC pathway in B16-F10 and Mel 13 melanoma cell lines. a Analysis of cell number performed on B16-F10 and Mel 13, after treatment for 24 and 48 h with 15 μM 3 m3 or 10 −7 M αMSH in the presence or absence of 3 μM GW9662. In the combined treatment αMSH/GW9662 or 3 m3/GW9662, cells underwent a pre-treatment with GW9662 for 1 h. Cell number was expressed as a percent variation in comparison to the value of untreated control cells. Data are mean values ± SD of three independent experiments performed in triplicate. * p
    Anti Pparγ Rabbit Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 83/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    New England Biolabs antibody against phospho akt
    The <t>PPARγ</t> acts as an effector element in determining the reduction of cell number induced by the activation of PI(4,5)P2/PLC pathway in B16-F10 and Mel 13 melanoma cell lines. a Analysis of cell number performed on B16-F10 and Mel 13, after treatment for 24 and 48 h with 15 μM 3 m3 or 10 −7 M αMSH in the presence or absence of 3 μM GW9662. In the combined treatment αMSH/GW9662 or 3 m3/GW9662, cells underwent a pre-treatment with GW9662 for 1 h. Cell number was expressed as a percent variation in comparison to the value of untreated control cells. Data are mean values ± SD of three independent experiments performed in triplicate. * p
    Antibody Against Phospho Akt, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 81/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs o glycosidase
    The <t>PPARγ</t> acts as an effector element in determining the reduction of cell number induced by the activation of PI(4,5)P2/PLC pathway in B16-F10 and Mel 13 melanoma cell lines. a Analysis of cell number performed on B16-F10 and Mel 13, after treatment for 24 and 48 h with 15 μM 3 m3 or 10 −7 M αMSH in the presence or absence of 3 μM GW9662. In the combined treatment αMSH/GW9662 or 3 m3/GW9662, cells underwent a pre-treatment with GW9662 for 1 h. Cell number was expressed as a percent variation in comparison to the value of untreated control cells. Data are mean values ± SD of three independent experiments performed in triplicate. * p
    O Glycosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs pngase f treatment infected cell
    The <t>PPARγ</t> acts as an effector element in determining the reduction of cell number induced by the activation of PI(4,5)P2/PLC pathway in B16-F10 and Mel 13 melanoma cell lines. a Analysis of cell number performed on B16-F10 and Mel 13, after treatment for 24 and 48 h with 15 μM 3 m3 or 10 −7 M αMSH in the presence or absence of 3 μM GW9662. In the combined treatment αMSH/GW9662 or 3 m3/GW9662, cells underwent a pre-treatment with GW9662 for 1 h. Cell number was expressed as a percent variation in comparison to the value of untreated control cells. Data are mean values ± SD of three independent experiments performed in triplicate. * p
    Pngase F Treatment Infected Cell, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    New England Biolabs baculovirus infected sf9 cells
    The <t>PPARγ</t> acts as an effector element in determining the reduction of cell number induced by the activation of PI(4,5)P2/PLC pathway in B16-F10 and Mel 13 melanoma cell lines. a Analysis of cell number performed on B16-F10 and Mel 13, after treatment for 24 and 48 h with 15 μM 3 m3 or 10 −7 M αMSH in the presence or absence of 3 μM GW9662. In the combined treatment αMSH/GW9662 or 3 m3/GW9662, cells underwent a pre-treatment with GW9662 for 1 h. Cell number was expressed as a percent variation in comparison to the value of untreated control cells. Data are mean values ± SD of three independent experiments performed in triplicate. * p
    Baculovirus Infected Sf9 Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    New England Biolabs mouse anti mbp antibody
    ELISA and competitive ELISA assays demonstrated WSSV VP31 binding to the <t>PmLamr</t> was specific. (A) ELISA analysis of <t>MBP-VP31</t> binding to the PmLamr-His in a dose-dependent manner. PmLamr-His (2 μg/ well) was coated to a 96-well plate and incubated with various amounts of purified MBP-VP31. MBP was added as negative control. (B) In competitive ELISA analysis, binding of MBP-VP31 and PmLamr-His were competed by WSSV virion proteins. PmLamr-His (2 μg/well) was coated to a 96-well plate. MBP-VP31 (200 ng) with various amounts of purified WSSV virion proteins (10–1200 ng) were added to wells and incubated. Addition of BSA (10–1200 ng) to wells served as a negative control. Mean (± SD) from three replicates.
    Mouse Anti Mbp Antibody, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs bsa
    ELISA and competitive ELISA assays demonstrated WSSV VP31 binding to the <t>PmLamr</t> was specific. (A) ELISA analysis of <t>MBP-VP31</t> binding to the PmLamr-His in a dose-dependent manner. PmLamr-His (2 μg/ well) was coated to a 96-well plate and incubated with various amounts of purified MBP-VP31. MBP was added as negative control. (B) In competitive ELISA analysis, binding of MBP-VP31 and PmLamr-His were competed by WSSV virion proteins. PmLamr-His (2 μg/well) was coated to a 96-well plate. MBP-VP31 (200 ng) with various amounts of purified WSSV virion proteins (10–1200 ng) were added to wells and incubated. Addition of BSA (10–1200 ng) to wells served as a negative control. Mean (± SD) from three replicates.
    Bsa, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 3207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Changes in HDAC6 and TP53BP1 transcript and protein levels in response to hypoxia. ( a ) Expression across introns in HDAC6 at 0, 2, 24 h following a shift to reduced oxygen (1%). Colour represents log 2 fold-change. ( b ) HDAC6 protein levels as determined by western blot ( c ). ( d–f ) TP53BP1 exhibits similar patterns of expression.

    Journal: NPJ genomic medicine

    Article Title: Hypoxia-driven splicing into noncoding isoforms regulates the DNA damage response

    doi: 10.1038/npjgenmed.2016.20

    Figure Lengend Snippet: Changes in HDAC6 and TP53BP1 transcript and protein levels in response to hypoxia. ( a ) Expression across introns in HDAC6 at 0, 2, 24 h following a shift to reduced oxygen (1%). Colour represents log 2 fold-change. ( b ) HDAC6 protein levels as determined by western blot ( c ). ( d–f ) TP53BP1 exhibits similar patterns of expression.

    Article Snippet: The membrane was blocked in 5%milk PBS-T for 30 min and blotted overnight with HDAC6 antibody (1/1,000 NEB 7558S), TP53BP1 (1/1,000 AT4311a Generon mouse monoclonal antibody), or tubulin antibody (1/5,000 Sigma T6199).

    Techniques: Expressing, Western Blot

    The PPARγ acts as an effector element in determining the reduction of cell number induced by the activation of PI(4,5)P2/PLC pathway in B16-F10 and Mel 13 melanoma cell lines. a Analysis of cell number performed on B16-F10 and Mel 13, after treatment for 24 and 48 h with 15 μM 3 m3 or 10 −7 M αMSH in the presence or absence of 3 μM GW9662. In the combined treatment αMSH/GW9662 or 3 m3/GW9662, cells underwent a pre-treatment with GW9662 for 1 h. Cell number was expressed as a percent variation in comparison to the value of untreated control cells. Data are mean values ± SD of three independent experiments performed in triplicate. * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: The α-melanocyte stimulating hormone/peroxisome proliferator activated receptor-γ pathway down-regulates proliferation in melanoma cell lines

    doi: 10.1186/s13046-017-0611-4

    Figure Lengend Snippet: The PPARγ acts as an effector element in determining the reduction of cell number induced by the activation of PI(4,5)P2/PLC pathway in B16-F10 and Mel 13 melanoma cell lines. a Analysis of cell number performed on B16-F10 and Mel 13, after treatment for 24 and 48 h with 15 μM 3 m3 or 10 −7 M αMSH in the presence or absence of 3 μM GW9662. In the combined treatment αMSH/GW9662 or 3 m3/GW9662, cells underwent a pre-treatment with GW9662 for 1 h. Cell number was expressed as a percent variation in comparison to the value of untreated control cells. Data are mean values ± SD of three independent experiments performed in triplicate. * p

    Article Snippet: Immunofluorescence analysis Cells grown on coverslips previously coated with 2% gelatin on 24-well plates were treated with 3 M3 (15 μM) for 1, 3 and 6 h. For immunolabelling with anti-PPARγ rabbit antibody (1:50 in PBS) (Cell Signalling Tecnology, New England Biolabs, UK) cells were fixed in methanol for 4 min. at −20 °C.

    Techniques: Activation Assay, Planar Chromatography

    Analysis of PPARγ translocation into the nucleus and activity in response to 3 M3 exposure. ( a , b , c ) Immunofluorescence analysis of PPARγ localization in untreated cells ( A - C , G - I ) and in cells treated with 15 μM 3 M3 for 3 h ( D - F , J - L ). Immunolabeling with anti-PPARγ antibody and nuclear staining with DAPI. Scale bar: 20 μM. ( c ) Quantitative analysis of the PPARγ/DAPI colocalization signal in the nucleus. Results are express as fold increase of colocalization signal with respect to the values obtained in untreated cells and are reported as mean value ± SD (%) (* p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: The α-melanocyte stimulating hormone/peroxisome proliferator activated receptor-γ pathway down-regulates proliferation in melanoma cell lines

    doi: 10.1186/s13046-017-0611-4

    Figure Lengend Snippet: Analysis of PPARγ translocation into the nucleus and activity in response to 3 M3 exposure. ( a , b , c ) Immunofluorescence analysis of PPARγ localization in untreated cells ( A - C , G - I ) and in cells treated with 15 μM 3 M3 for 3 h ( D - F , J - L ). Immunolabeling with anti-PPARγ antibody and nuclear staining with DAPI. Scale bar: 20 μM. ( c ) Quantitative analysis of the PPARγ/DAPI colocalization signal in the nucleus. Results are express as fold increase of colocalization signal with respect to the values obtained in untreated cells and are reported as mean value ± SD (%) (* p

    Article Snippet: Immunofluorescence analysis Cells grown on coverslips previously coated with 2% gelatin on 24-well plates were treated with 3 M3 (15 μM) for 1, 3 and 6 h. For immunolabelling with anti-PPARγ rabbit antibody (1:50 in PBS) (Cell Signalling Tecnology, New England Biolabs, UK) cells were fixed in methanol for 4 min. at −20 °C.

    Techniques: Translocation Assay, Activity Assay, Immunofluorescence, Immunolabeling, Staining

    Analysis of PPARγ transcriptional activity in response to αMSH exposure in B16-F10, Mel 13 and NHMs. Cells were transfected with pGL3-(Jwt)3TKLuc reporter construct. After 24 h of transfection, cells were treated with 10 −7 M αMSH. The measurement of luciferase activity was carried out 30 min, 1, 3 and 6 h after treatment. The variability of transfection was normalized with renilla luciferase activity. The results were expressed as fold change with respect to untreated cells. Data are mean values ± SD of three independent experiments performed in triplicate, * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: The α-melanocyte stimulating hormone/peroxisome proliferator activated receptor-γ pathway down-regulates proliferation in melanoma cell lines

    doi: 10.1186/s13046-017-0611-4

    Figure Lengend Snippet: Analysis of PPARγ transcriptional activity in response to αMSH exposure in B16-F10, Mel 13 and NHMs. Cells were transfected with pGL3-(Jwt)3TKLuc reporter construct. After 24 h of transfection, cells were treated with 10 −7 M αMSH. The measurement of luciferase activity was carried out 30 min, 1, 3 and 6 h after treatment. The variability of transfection was normalized with renilla luciferase activity. The results were expressed as fold change with respect to untreated cells. Data are mean values ± SD of three independent experiments performed in triplicate, * p

    Article Snippet: Immunofluorescence analysis Cells grown on coverslips previously coated with 2% gelatin on 24-well plates were treated with 3 M3 (15 μM) for 1, 3 and 6 h. For immunolabelling with anti-PPARγ rabbit antibody (1:50 in PBS) (Cell Signalling Tecnology, New England Biolabs, UK) cells were fixed in methanol for 4 min. at −20 °C.

    Techniques: Activity Assay, Transfection, Construct, Luciferase

    ELISA and competitive ELISA assays demonstrated WSSV VP31 binding to the PmLamr was specific. (A) ELISA analysis of MBP-VP31 binding to the PmLamr-His in a dose-dependent manner. PmLamr-His (2 μg/ well) was coated to a 96-well plate and incubated with various amounts of purified MBP-VP31. MBP was added as negative control. (B) In competitive ELISA analysis, binding of MBP-VP31 and PmLamr-His were competed by WSSV virion proteins. PmLamr-His (2 μg/well) was coated to a 96-well plate. MBP-VP31 (200 ng) with various amounts of purified WSSV virion proteins (10–1200 ng) were added to wells and incubated. Addition of BSA (10–1200 ng) to wells served as a negative control. Mean (± SD) from three replicates.

    Journal: PLoS ONE

    Article Title: Laminin Receptor in Shrimp Is a Cellular Attachment Receptor for White Spot Syndrome Virus

    doi: 10.1371/journal.pone.0156375

    Figure Lengend Snippet: ELISA and competitive ELISA assays demonstrated WSSV VP31 binding to the PmLamr was specific. (A) ELISA analysis of MBP-VP31 binding to the PmLamr-His in a dose-dependent manner. PmLamr-His (2 μg/ well) was coated to a 96-well plate and incubated with various amounts of purified MBP-VP31. MBP was added as negative control. (B) In competitive ELISA analysis, binding of MBP-VP31 and PmLamr-His were competed by WSSV virion proteins. PmLamr-His (2 μg/well) was coated to a 96-well plate. MBP-VP31 (200 ng) with various amounts of purified WSSV virion proteins (10–1200 ng) were added to wells and incubated. Addition of BSA (10–1200 ng) to wells served as a negative control. Mean (± SD) from three replicates.

    Article Snippet: After blocking with PBS containing 5% bovine serum albumin and 2% normal goat serum for a duration of 16 h at 4°C, the cells were then incubated for a total of 3 h at room temperature (RT) with either the 1:500 PBS-diluted rabbit anti-PmLamr antibody, the 1:500 PBS-diluted mouse anti-MBP antibody (NEB), or with both antibodies.

    Techniques: Enzyme-linked Immunosorbent Assay, Competitive ELISA, Binding Assay, Incubation, Purification, Negative Control

    PmLamr interacted with WSSV VP31. Protein pull-down assays of PmLamr-His with MBP-VP31. (A) SDS-PAGE of purified MBP, MBP-VP31 and PmLamr-His proteins. (B) PmLamr-His was incubated with amylose resins (Lane 1), MBP- (Lane 2), or MBP-VP31- (Lane 3) conjugated to amylose resins, pelleted, washed, and detected by immunoblotting (anti-PmLamr antibody).

    Journal: PLoS ONE

    Article Title: Laminin Receptor in Shrimp Is a Cellular Attachment Receptor for White Spot Syndrome Virus

    doi: 10.1371/journal.pone.0156375

    Figure Lengend Snippet: PmLamr interacted with WSSV VP31. Protein pull-down assays of PmLamr-His with MBP-VP31. (A) SDS-PAGE of purified MBP, MBP-VP31 and PmLamr-His proteins. (B) PmLamr-His was incubated with amylose resins (Lane 1), MBP- (Lane 2), or MBP-VP31- (Lane 3) conjugated to amylose resins, pelleted, washed, and detected by immunoblotting (anti-PmLamr antibody).

    Article Snippet: After blocking with PBS containing 5% bovine serum albumin and 2% normal goat serum for a duration of 16 h at 4°C, the cells were then incubated for a total of 3 h at room temperature (RT) with either the 1:500 PBS-diluted rabbit anti-PmLamr antibody, the 1:500 PBS-diluted mouse anti-MBP antibody (NEB), or with both antibodies.

    Techniques: SDS Page, Purification, Incubation

    Cumulative post-challenge shrimp mortality. Shrimp were challenged with inoculum containing WSSV mixed with PmLamr-His (WSSV+PmLamr-His), or inoculum containing WSSV mixed with MBP-VP31 (WSSV+MBP-VP31). Asterisks indicated differences between positive and experimental groups (**P

    Journal: PLoS ONE

    Article Title: Laminin Receptor in Shrimp Is a Cellular Attachment Receptor for White Spot Syndrome Virus

    doi: 10.1371/journal.pone.0156375

    Figure Lengend Snippet: Cumulative post-challenge shrimp mortality. Shrimp were challenged with inoculum containing WSSV mixed with PmLamr-His (WSSV+PmLamr-His), or inoculum containing WSSV mixed with MBP-VP31 (WSSV+MBP-VP31). Asterisks indicated differences between positive and experimental groups (**P

    Article Snippet: After blocking with PBS containing 5% bovine serum albumin and 2% normal goat serum for a duration of 16 h at 4°C, the cells were then incubated for a total of 3 h at room temperature (RT) with either the 1:500 PBS-diluted rabbit anti-PmLamr antibody, the 1:500 PBS-diluted mouse anti-MBP antibody (NEB), or with both antibodies.

    Techniques:

    Immunofluorescence images; note colocalization of PmLamr-V5-His with MBP-VP31 on cells. Drosophila S2 cells were transfected with pDHsp/PmLamr-V5-His. At 48 h after transfection, 1 μg of MBP-VP31 was added. The presence of (A) PmLamr-V5-His (red) is visualized by rabbit anti-PmLamr antibody and Cy3 dye-conjugated goat anti-rabbit IgG antibody. As for (B) MBP-VP31 (green), mouse anti-MBP antibody and Alexa Fluor ® 488 dye-conjugated goat anti-mouse IgG antibody were used. (C) The nuclear DNA was counterstained by DAPI. (D) Merged Cy3, Alexa Fluor ® 488, and DAPI signals. Scale bar = 10 μm.

    Journal: PLoS ONE

    Article Title: Laminin Receptor in Shrimp Is a Cellular Attachment Receptor for White Spot Syndrome Virus

    doi: 10.1371/journal.pone.0156375

    Figure Lengend Snippet: Immunofluorescence images; note colocalization of PmLamr-V5-His with MBP-VP31 on cells. Drosophila S2 cells were transfected with pDHsp/PmLamr-V5-His. At 48 h after transfection, 1 μg of MBP-VP31 was added. The presence of (A) PmLamr-V5-His (red) is visualized by rabbit anti-PmLamr antibody and Cy3 dye-conjugated goat anti-rabbit IgG antibody. As for (B) MBP-VP31 (green), mouse anti-MBP antibody and Alexa Fluor ® 488 dye-conjugated goat anti-mouse IgG antibody were used. (C) The nuclear DNA was counterstained by DAPI. (D) Merged Cy3, Alexa Fluor ® 488, and DAPI signals. Scale bar = 10 μm.

    Article Snippet: After blocking with PBS containing 5% bovine serum albumin and 2% normal goat serum for a duration of 16 h at 4°C, the cells were then incubated for a total of 3 h at room temperature (RT) with either the 1:500 PBS-diluted rabbit anti-PmLamr antibody, the 1:500 PBS-diluted mouse anti-MBP antibody (NEB), or with both antibodies.

    Techniques: Immunofluorescence, Transfection