pbs Search Results


li cor intercept antibody diluent  (LI-COR)
95
LI-COR li cor intercept antibody diluent
Li Cor Intercept Antibody Diluent, supplied by LI-COR, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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li cor intercept antibody diluent - by Bioz Stars, 2025-07
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twicewith pbs buffer  (GE Healthcare)
95
GE Healthcare twicewith pbs buffer
Twicewith Pbs Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
twicewith pbs buffer - by Bioz Stars, 2025-07
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intercept pbs blocking buffer  (LI-COR)
99
LI-COR intercept pbs blocking buffer
Intercept Pbs Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
intercept pbs blocking buffer - by Bioz Stars, 2025-07
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pbs hsp70 cas9 plasmid  (Addgene inc)
94
Addgene inc pbs hsp70 cas9 plasmid
(A) Sequence cassette containing 24x MS2 and SV40 poly(A) signal was inserted into the 3′ UTR of target genes by using <t>CRISPR/Cas9.</t> (B) Fluorescent in situ hybridization of endogenous hb-MS2 . Embryo at nc14 was shown. MS2 probe was used for the analysis. Image was cropped and rotated to align embryo (anterior to the left and posterior to the right). Scale bar indicates 50 μm. (C-E) Profiles of total RNA production (C), transcription period (D) and transcription rate (E) as a function of AP position. Line plot indicates mean values in groups of nuclei binned by AP position. In total, 695 nuclei from three independent embryos were analyzed. (F) Representative trajectories of transcription activity of hb-MS2 in individual nuclei. (G) A cumulative plot showing fraction of bursting events (y axis) and length of transcriptional bursting (x axis). A total of 230 nuclei from three independent embryos was analyzed.
Pbs Hsp70 Cas9 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs hsp70 cas9 plasmid/product/Addgene inc
Average 94 stars, based on 1 article reviews
pbs hsp70 cas9 plasmid - by Bioz Stars, 2025-07
94/100 stars
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pbs tx  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology pbs tx
(A) Sequence cassette containing 24x MS2 and SV40 poly(A) signal was inserted into the 3′ UTR of target genes by using <t>CRISPR/Cas9.</t> (B) Fluorescent in situ hybridization of endogenous hb-MS2 . Embryo at nc14 was shown. MS2 probe was used for the analysis. Image was cropped and rotated to align embryo (anterior to the left and posterior to the right). Scale bar indicates 50 μm. (C-E) Profiles of total RNA production (C), transcription period (D) and transcription rate (E) as a function of AP position. Line plot indicates mean values in groups of nuclei binned by AP position. In total, 695 nuclei from three independent embryos were analyzed. (F) Representative trajectories of transcription activity of hb-MS2 in individual nuclei. (G) A cumulative plot showing fraction of bursting events (y axis) and length of transcriptional bursting (x axis). A total of 230 nuclei from three independent embryos was analyzed.
Pbs Tx, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs tx/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
pbs tx - by Bioz Stars, 2025-07
96/100 stars
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Image Search Results


(A) Sequence cassette containing 24x MS2 and SV40 poly(A) signal was inserted into the 3′ UTR of target genes by using CRISPR/Cas9. (B) Fluorescent in situ hybridization of endogenous hb-MS2 . Embryo at nc14 was shown. MS2 probe was used for the analysis. Image was cropped and rotated to align embryo (anterior to the left and posterior to the right). Scale bar indicates 50 μm. (C-E) Profiles of total RNA production (C), transcription period (D) and transcription rate (E) as a function of AP position. Line plot indicates mean values in groups of nuclei binned by AP position. In total, 695 nuclei from three independent embryos were analyzed. (F) Representative trajectories of transcription activity of hb-MS2 in individual nuclei. (G) A cumulative plot showing fraction of bursting events (y axis) and length of transcriptional bursting (x axis). A total of 230 nuclei from three independent embryos was analyzed.

Journal: bioRxiv

Article Title: Dynamic regulation of anterior-posterior patterning genes in living Drosophila embryos

doi: 10.1101/2020.11.25.395277

Figure Lengend Snippet: (A) Sequence cassette containing 24x MS2 and SV40 poly(A) signal was inserted into the 3′ UTR of target genes by using CRISPR/Cas9. (B) Fluorescent in situ hybridization of endogenous hb-MS2 . Embryo at nc14 was shown. MS2 probe was used for the analysis. Image was cropped and rotated to align embryo (anterior to the left and posterior to the right). Scale bar indicates 50 μm. (C-E) Profiles of total RNA production (C), transcription period (D) and transcription rate (E) as a function of AP position. Line plot indicates mean values in groups of nuclei binned by AP position. In total, 695 nuclei from three independent embryos were analyzed. (F) Representative trajectories of transcription activity of hb-MS2 in individual nuclei. (G) A cumulative plot showing fraction of bursting events (y axis) and length of transcriptional bursting (x axis). A total of 230 nuclei from three independent embryos was analyzed.

Article Snippet: pCFD3 gRNA expression plasmids, pBS-3xP3-GFP donor plasmid and pBS-hsp70-Cas9 plasmid (addgene #46294) were co-injected to homozygote hb-MS2 embryos.

Techniques: Sequencing, CRISPR, In Situ Hybridization, Activity Assay