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Image Search Results

Journal: bioRxiv
Article Title: Dynamic regulation of anterior-posterior patterning genes in living Drosophila embryos
doi: 10.1101/2020.11.25.395277
Figure Lengend Snippet: (A) Sequence cassette containing 24x MS2 and SV40 poly(A) signal was inserted into the 3′ UTR of target genes by using CRISPR/Cas9. (B) Fluorescent in situ hybridization of endogenous hb-MS2 . Embryo at nc14 was shown. MS2 probe was used for the analysis. Image was cropped and rotated to align embryo (anterior to the left and posterior to the right). Scale bar indicates 50 μm. (C-E) Profiles of total RNA production (C), transcription period (D) and transcription rate (E) as a function of AP position. Line plot indicates mean values in groups of nuclei binned by AP position. In total, 695 nuclei from three independent embryos were analyzed. (F) Representative trajectories of transcription activity of hb-MS2 in individual nuclei. (G) A cumulative plot showing fraction of bursting events (y axis) and length of transcriptional bursting (x axis). A total of 230 nuclei from three independent embryos was analyzed.
Article Snippet: pCFD3 gRNA expression plasmids, pBS-3xP3-GFP donor plasmid and
Techniques: Sequencing, CRISPR, In Situ Hybridization, Activity Assay