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  • 95
    Millipore phosphate buffer saline pbs components
    Square wave voltammograms of a gold electrode modified by <t>MBT/DPM–Cu</t> (II) (solid line), after immobilization of His 6 -H1 HA monomer (dashed lines); and after filling free spaces with bovine serum albumin (finely dashed lines). Measurement conditions: 0.01 M <t>PBS</t> (pH 7.4), scan rate 100mVs − 1
    Phosphate Buffer Saline Pbs Components, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher pbs
    Toxicity screening of <t>DMSO-PBS</t> mixtures. (a) Fluorescence micrographs of cell suspension droplets exposed to DMSO-PBS mixtures. After the exposure, the droplets were mixed with viability dye droplets to stain all cells with Hoechst and injured cells with
    Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 45702 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bio-Rad phosphate buffered saline pbs
    Membrane association of LidA after translocation into host cells. (A) L. pneumophila (WT) and an isogenic dotA mutant ( dotA ), both expressing <t>GFP,</t> were grown to postexponential phase and resuspended in <t>PBS</t> containing either 1% Triton X-100, 1% CHAPS,
    Phosphate Buffered Saline Pbs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Boster Bio phosphate buffered saline pbs
    Membrane association of LidA after translocation into host cells. (A) L. pneumophila (WT) and an isogenic dotA mutant ( dotA ), both expressing <t>GFP,</t> were grown to postexponential phase and resuspended in <t>PBS</t> containing either 1% Triton X-100, 1% CHAPS,
    Phosphate Buffered Saline Pbs, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Cell Signaling Technology Inc phosphate buffered saline pbs
    <t>Metformin</t> inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or <t>PBS</t> by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,
    Phosphate Buffered Saline Pbs, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TaKaRa phosphate buffered saline pbs
    <t>Metformin</t> inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or <t>PBS</t> by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,
    Phosphate Buffered Saline Pbs, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher phosphate buffered saline pbs
    EMT with proliferation induced by EGTA with <t>EGF+FGF-2</t> is caused by Wnt/β-catenin signaling by rescued by SS3Y β-catenin. (a) The TCF/LEF reporter activity was silent in cells treated by <t>PBS,</t> EGTA, EGF, FGF-2, TGF-β1, or EGF+FGF-2+TGF-β1 but elevated 15-fold by EGTA with EGF+FGF-2. The latter was abolished by XAV939 (*, P
    Phosphate Buffered Saline Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 27245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioVision phosphate buffered saline pbs
    ZD55-IL-18 and DTIC increased <t>apoptosis</t> of A375 cells. Notes: ( A ) At 48 hours after treatment, apoptotic cells were detected by the condensation of nuclear chromatin and its fragmentation. Magnification ×400. ( B ) Quantitative analysis of apoptotic A375 cells in different treatment groups. Scale bar 10 µm. Abbreviations: DTIC, dacarbazine; <t>PBS,</t> phosphate-buffered saline; IL-18, interleukin-18.
    Phosphate Buffered Saline Pbs, supplied by BioVision, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Rad triton x 100 phosphate buffered saline
    ZD55-IL-18 and DTIC increased <t>apoptosis</t> of A375 cells. Notes: ( A ) At 48 hours after treatment, apoptotic cells were detected by the condensation of nuclear chromatin and its fragmentation. Magnification ×400. ( B ) Quantitative analysis of apoptotic A375 cells in different treatment groups. Scale bar 10 µm. Abbreviations: DTIC, dacarbazine; <t>PBS,</t> phosphate-buffered saline; IL-18, interleukin-18.
    Triton X 100 Phosphate Buffered Saline, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pbs  (Lonza)
    95
    Lonza pbs
    CAP256 gp140-FL-IP-His protein α-Env <t>ELISA.</t> Binding ELISAs using anti-Env human monoclonal antibodies to CAP256 gp140-FL-IP-His. (A) PGT128 and PGT135 binding indicate the presence of the Env V3-glycan supersite. Similarly, the CD4 binding site using VRC01 The V2-glycan epitope was detected with PG9 (A), but no binding was observed for the native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08 (B). In line with this, clear ELISA signals were observed for F105 and 446-52D, indicative of the presence of misfolded Env (A). (C) Control soluble, trimeric BG505_664-His Env protein performed as expected, with ELISA signals for native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08. (D) No binding of MAbs was observed for the no protein <t>(PBS)</t> control.
    Pbs, supplied by Lonza, used in various techniques. Bioz Stars score: 95/100, based on 1233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    APP Pharmaceuticals phosphate buffered saline pbs
    CAP256 gp140-FL-IP-His protein α-Env <t>ELISA.</t> Binding ELISAs using anti-Env human monoclonal antibodies to CAP256 gp140-FL-IP-His. (A) PGT128 and PGT135 binding indicate the presence of the Env V3-glycan supersite. Similarly, the CD4 binding site using VRC01 The V2-glycan epitope was detected with PG9 (A), but no binding was observed for the native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08 (B). In line with this, clear ELISA signals were observed for F105 and 446-52D, indicative of the presence of misfolded Env (A). (C) Control soluble, trimeric BG505_664-His Env protein performed as expected, with ELISA signals for native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08. (D) No binding of MAbs was observed for the no protein <t>(PBS)</t> control.
    Phosphate Buffered Saline Pbs, supplied by APP Pharmaceuticals, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Atlanta Biologicals phosphate buffered saline pbs
    CAP256 gp140-FL-IP-His protein α-Env <t>ELISA.</t> Binding ELISAs using anti-Env human monoclonal antibodies to CAP256 gp140-FL-IP-His. (A) PGT128 and PGT135 binding indicate the presence of the Env V3-glycan supersite. Similarly, the CD4 binding site using VRC01 The V2-glycan epitope was detected with PG9 (A), but no binding was observed for the native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08 (B). In line with this, clear ELISA signals were observed for F105 and 446-52D, indicative of the presence of misfolded Env (A). (C) Control soluble, trimeric BG505_664-His Env protein performed as expected, with ELISA signals for native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08. (D) No binding of MAbs was observed for the no protein <t>(PBS)</t> control.
    Phosphate Buffered Saline Pbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Becton Dickinson phosphate buffered saline pbs
    Evaluation of cellular toxicity and viability in cisplatin-treated HK-2 cells. HK-2 cells were treated with increasing concentrations of cisplatin (6.25–100 μM). (A) HK-2 cell proliferation was assessed by MTS assay after 6, 12, and 24 h of cisplatin treatment. (B) Cells were treated with the indicated concentrations of cisplatin for 24 h and cell death was measured. (C and D) HK-2 cells were washed twice with cold phosphate-buffered saline <t>(PBS),</t> stained with Annexin V/propidium iodide (PI), and cell death was determined by flow <t>cytometry.</t> (E and F) Cisplatin-induced cell viability and cytotoxicity assay using calcein AM and ethidium homodimer (EthD-1) fluorescence staining with confocal microscopy (E) and flow cytometry (F). Magnification of the images was ×40 and scale bars represent 75 μm. The mean ± S.D. (standard deviation) of three independent experiments are presented; * p
    Phosphate Buffered Saline Pbs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 6701 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Beijing Solarbio Science phosphate buffered saline pbs
    Evaluation of cellular toxicity and viability in cisplatin-treated HK-2 cells. HK-2 cells were treated with increasing concentrations of cisplatin (6.25–100 μM). (A) HK-2 cell proliferation was assessed by MTS assay after 6, 12, and 24 h of cisplatin treatment. (B) Cells were treated with the indicated concentrations of cisplatin for 24 h and cell death was measured. (C and D) HK-2 cells were washed twice with cold phosphate-buffered saline <t>(PBS),</t> stained with Annexin V/propidium iodide (PI), and cell death was determined by flow <t>cytometry.</t> (E and F) Cisplatin-induced cell viability and cytotoxicity assay using calcein AM and ethidium homodimer (EthD-1) fluorescence staining with confocal microscopy (E) and flow cytometry (F). Magnification of the images was ×40 and scale bars represent 75 μm. The mean ± S.D. (standard deviation) of three independent experiments are presented; * p
    Phosphate Buffered Saline Pbs, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio Basic Canada phosphate buffered saline pbs
    Evaluation of cellular toxicity and viability in cisplatin-treated HK-2 cells. HK-2 cells were treated with increasing concentrations of cisplatin (6.25–100 μM). (A) HK-2 cell proliferation was assessed by MTS assay after 6, 12, and 24 h of cisplatin treatment. (B) Cells were treated with the indicated concentrations of cisplatin for 24 h and cell death was measured. (C and D) HK-2 cells were washed twice with cold phosphate-buffered saline <t>(PBS),</t> stained with Annexin V/propidium iodide (PI), and cell death was determined by flow <t>cytometry.</t> (E and F) Cisplatin-induced cell viability and cytotoxicity assay using calcein AM and ethidium homodimer (EthD-1) fluorescence staining with confocal microscopy (E) and flow cytometry (F). Magnification of the images was ×40 and scale bars represent 75 μm. The mean ± S.D. (standard deviation) of three independent experiments are presented; * p
    Phosphate Buffered Saline Pbs, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biochrom phosphate buffered saline pbs
    Evaluation of cellular toxicity and viability in cisplatin-treated HK-2 cells. HK-2 cells were treated with increasing concentrations of cisplatin (6.25–100 μM). (A) HK-2 cell proliferation was assessed by MTS assay after 6, 12, and 24 h of cisplatin treatment. (B) Cells were treated with the indicated concentrations of cisplatin for 24 h and cell death was measured. (C and D) HK-2 cells were washed twice with cold phosphate-buffered saline <t>(PBS),</t> stained with Annexin V/propidium iodide (PI), and cell death was determined by flow <t>cytometry.</t> (E and F) Cisplatin-induced cell viability and cytotoxicity assay using calcein AM and ethidium homodimer (EthD-1) fluorescence staining with confocal microscopy (E) and flow cytometry (F). Magnification of the images was ×40 and scale bars represent 75 μm. The mean ± S.D. (standard deviation) of three independent experiments are presented; * p
    Phosphate Buffered Saline Pbs, supplied by Biochrom, used in various techniques. Bioz Stars score: 99/100, based on 359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biofluids Inc phosphate buffered saline pbs
    Evaluation of cellular toxicity and viability in cisplatin-treated HK-2 cells. HK-2 cells were treated with increasing concentrations of cisplatin (6.25–100 μM). (A) HK-2 cell proliferation was assessed by MTS assay after 6, 12, and 24 h of cisplatin treatment. (B) Cells were treated with the indicated concentrations of cisplatin for 24 h and cell death was measured. (C and D) HK-2 cells were washed twice with cold phosphate-buffered saline <t>(PBS),</t> stained with Annexin V/propidium iodide (PI), and cell death was determined by flow <t>cytometry.</t> (E and F) Cisplatin-induced cell viability and cytotoxicity assay using calcein AM and ethidium homodimer (EthD-1) fluorescence staining with confocal microscopy (E) and flow cytometry (F). Magnification of the images was ×40 and scale bars represent 75 μm. The mean ± S.D. (standard deviation) of three independent experiments are presented; * p
    Phosphate Buffered Saline Pbs, supplied by Biofluids Inc, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bioMerieux phosphate buffered saline pbs
    Evaluation of cellular toxicity and viability in cisplatin-treated HK-2 cells. HK-2 cells were treated with increasing concentrations of cisplatin (6.25–100 μM). (A) HK-2 cell proliferation was assessed by MTS assay after 6, 12, and 24 h of cisplatin treatment. (B) Cells were treated with the indicated concentrations of cisplatin for 24 h and cell death was measured. (C and D) HK-2 cells were washed twice with cold phosphate-buffered saline <t>(PBS),</t> stained with Annexin V/propidium iodide (PI), and cell death was determined by flow <t>cytometry.</t> (E and F) Cisplatin-induced cell viability and cytotoxicity assay using calcein AM and ethidium homodimer (EthD-1) fluorescence staining with confocal microscopy (E) and flow cytometry (F). Magnification of the images was ×40 and scale bars represent 75 μm. The mean ± S.D. (standard deviation) of three independent experiments are presented; * p
    Phosphate Buffered Saline Pbs, supplied by bioMerieux, used in various techniques. Bioz Stars score: 99/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Boehringer Mannheim phosphate buffered saline pbs
    Effect of diosgenin on SHh, SMO and GLI1 gene expression during megakaryocytic differentiation in HEL and TF1a cell lines. (A) Cells were treated or not with 10 µM diosgenin for 24, 48, 72 and 96 h then SHh, SMO and GLI1 genes expressions were evaluated. Total RNA was extracted and 2µg of total RNA were transcribed into cDNA and used for PCR. PCR resulting fragments were visualized by electrophoresis on a 1% agarose gel containing ethidium bromide. Quantification of SHh, SMO and GLI1 transcripts were normalized to 18S as an internal control. The agarose gels shown are representative of six separate experiments. (B) Cells were treated or not with 10µM diosgenin for 96 h and megakaryocytic differentiation was evaluated by analyzing nuclear ploidy. Cells were fixed and <t>permeabilized</t> in 70% ethanol in <t>PBS</t> at −20 °C overnight, washed in PBS, treated with RNase and stained with PI. Then, flow cytometric analyses (FC) were performed to analyze DNA content.
    Phosphate Buffered Saline Pbs, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 99/100, based on 389 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cambrex phosphate buffered saline pbs
    Survival of C3-deficient mice challenged i.n. with WU2 after i.p. administration of <t>PPS3-binding</t> MAbs. (A) C3 KO mice. Survival of C3 KO mice that received 10 μg 1E2, 5F6, or 7A9 was significantly longer than that of <t>PBS-treated</t> animals (*,
    Phosphate Buffered Saline Pbs, supplied by Cambrex, used in various techniques. Bioz Stars score: 99/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Roth GmbH phosphate buffered saline pbs
    Survival of C3-deficient mice challenged i.n. with WU2 after i.p. administration of <t>PPS3-binding</t> MAbs. (A) C3 KO mice. Survival of C3 KO mice that received 10 μg 1E2, 5F6, or 7A9 was significantly longer than that of <t>PBS-treated</t> animals (*,
    Phosphate Buffered Saline Pbs, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 99/100, based on 229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellgro phosphate buffered saline pbs
    Survival of C3-deficient mice challenged i.n. with WU2 after i.p. administration of <t>PPS3-binding</t> MAbs. (A) C3 KO mice. Survival of C3 KO mice that received 10 μg 1E2, 5F6, or 7A9 was significantly longer than that of <t>PBS-treated</t> animals (*,
    Phosphate Buffered Saline Pbs, supplied by Cellgro, used in various techniques. Bioz Stars score: 99/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Corning Life Sciences phosphate buffered saline pbs
    Visualization of the fluorescence intensitites of the microarrays. The upper part shows a subset of the 269 control DBSS. The Swedish <t>C3</t> deficient and C3 positive serum standards contains 23 serial dilutions each (high to low dilution, 1∶5 to 1∶100 000). The two lowest dilutions of the standard curve are separated by a blank <t>(PBS).</t> The dotted rectangle depicts the C3 deficient sample.
    Phosphate Buffered Saline Pbs, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 99/100, based on 578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EuroClone phosphate buffered saline pbs
    Visualization of the fluorescence intensitites of the microarrays. The upper part shows a subset of the 269 control DBSS. The Swedish <t>C3</t> deficient and C3 positive serum standards contains 23 serial dilutions each (high to low dilution, 1∶5 to 1∶100 000). The two lowest dilutions of the standard curve are separated by a blank <t>(PBS).</t> The dotted rectangle depicts the C3 deficient sample.
    Phosphate Buffered Saline Pbs, supplied by EuroClone, used in various techniques. Bioz Stars score: 99/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific phosphate buffered saline pbs
    MTT Assay: A. Without Light: 1-AP exposed HaCaT cells remained in dark conditions for 20 min in 1× <t>PBS</t> buffer. After treatment, <t>DMEM</t> was added to the cells, and they underwent incubation at 37°C/ 5 %CO 2 for 0, 2, 4, 6, and 24 hrs (N=3).
    Phosphate Buffered Saline Pbs, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 99/100, based on 1052 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    FormuMax phosphate buffered saline pbs
    Formation of granulomas are necessary for the increased IL-1RA production within the liver of CFZ-treated mice. A) Liver cryosections of <t>PBS</t> or <t>clodronate</t> treated mice fed a control diet or CFZ-supplemented diet stained for F4/80 (green) and showing CLDI fluorescence. Treatment with clodronate reduces hepatic macrophages, resulting in reduced granuloma size and CLDI accumulation within the granuloma. B) IL-1RA production is inhibited within clodronate-CFZ treated mice, while it is significantly elevated in the PBS-CFZ treatment group. C) The granulomas which form ultimately are responsible for the production of IL-1RA, producing similar levels of IL-1RA to whole-organ homogenates. Scale bar is 50 um. (n=3-4 liver homogenates or isolated granulomas per treatment group) (*=p
    Phosphate Buffered Saline Pbs, supplied by FormuMax, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FUJIFILM phosphate buffered saline pbs
    Effects of U0126 or LY294002 or the combination of U1026 and LY294002 on severe combined immunodeficiency (SCID) mice bearing EHMES-10 cells. (A–C) Survival times of EHMES-10 cell-bearing SCID mice treated with U0126, LY294002, or the combination of U0126 and LY294002. EHMES-10 cells (3×10 6 ) were inoculated into the thoracic cavity of SCID mice. Seven days after inoculation, SCID mice were randomized into eight groups (n=7 mice/group) to receive vehicle (DMSO + <t>PBS),</t> U0126 (20, 30 and 40 mg/kg), or LY294002 (12.5, 25 and 50 mg/kg), or a combination of U0126 (30 mg/kg) and LY294002 (25 mg/kg). U, U0126; LY, LY294002; * P
    Phosphate Buffered Saline Pbs, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 99/100, based on 549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Dendritic protein synthesis is required for glycine-induced insertion of GluN2A ( A ) MAP2 staining of 25 DIV neurons cultured in a microfluidic device (CB: cell bodies, D: dendrites). Scale bar is 20 μm. ( B ) A representative dendritic region immunostained for MAP2 and GluN2A. Scale bar is 3 μm. ( C ) Time-lapse imaging shows microfluidic chambers maintain fluidic isolation throughout the glycine treatment paradigm. Microfluidic devices were filled with <t>PBS</t> (initial), then left-side PBS was replaced with PBS plus Cy3b dye (t 0 ), and, 30 min later, right-side PBS was replaced with PBS plus Cy5 dye (t 30 ). After 3 min, PBS plus Cy5 dye was replaced with PBS alone (t 33 ) and allowed to sit for an additional 30 min (t 60 ). The <t>Cy3</t> and Cy5 dye solutions remained restricted to the cell body (CB) and dendrite (sides), respectively, for the duration of the experiment. ( D ) Hippocampal neurons were cultured in microfluidic devices. Anisomycin was applied to either the cell body or the dendrite compartment for 30 min, followed by glycine or vehicle application to the dendrite compartment for 3 min, and an additional 30 min of incubation in solution without glycine. Anisomycin or DMSO was present in all solutions. Then, the neurons were fixed and immunostained for surface GluN2A protein (ANOVA, Bonferroni t-tests; n = 65; * p
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    Dendritic protein synthesis is required for glycine-induced insertion of GluN2A ( A ) MAP2 staining of 25 DIV neurons cultured in a microfluidic device (CB: cell bodies, D: dendrites). Scale bar is 20 μm. ( B ) A representative dendritic region immunostained for MAP2 and GluN2A. Scale bar is 3 μm. ( C ) Time-lapse imaging shows microfluidic chambers maintain fluidic isolation throughout the glycine treatment paradigm. Microfluidic devices were filled with <t>PBS</t> (initial), then left-side PBS was replaced with PBS plus Cy3b dye (t 0 ), and, 30 min later, right-side PBS was replaced with PBS plus Cy5 dye (t 30 ). After 3 min, PBS plus Cy5 dye was replaced with PBS alone (t 33 ) and allowed to sit for an additional 30 min (t 60 ). The <t>Cy3</t> and Cy5 dye solutions remained restricted to the cell body (CB) and dendrite (sides), respectively, for the duration of the experiment. ( D ) Hippocampal neurons were cultured in microfluidic devices. Anisomycin was applied to either the cell body or the dendrite compartment for 30 min, followed by glycine or vehicle application to the dendrite compartment for 3 min, and an additional 30 min of incubation in solution without glycine. Anisomycin or DMSO was present in all solutions. Then, the neurons were fixed and immunostained for surface GluN2A protein (ANOVA, Bonferroni t-tests; n = 65; * p
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    Mediatech phosphate buffered saline pbs
    Dendritic protein synthesis is required for glycine-induced insertion of GluN2A ( A ) MAP2 staining of 25 DIV neurons cultured in a microfluidic device (CB: cell bodies, D: dendrites). Scale bar is 20 μm. ( B ) A representative dendritic region immunostained for MAP2 and GluN2A. Scale bar is 3 μm. ( C ) Time-lapse imaging shows microfluidic chambers maintain fluidic isolation throughout the glycine treatment paradigm. Microfluidic devices were filled with <t>PBS</t> (initial), then left-side PBS was replaced with PBS plus Cy3b dye (t 0 ), and, 30 min later, right-side PBS was replaced with PBS plus Cy5 dye (t 30 ). After 3 min, PBS plus Cy5 dye was replaced with PBS alone (t 33 ) and allowed to sit for an additional 30 min (t 60 ). The <t>Cy3</t> and Cy5 dye solutions remained restricted to the cell body (CB) and dendrite (sides), respectively, for the duration of the experiment. ( D ) Hippocampal neurons were cultured in microfluidic devices. Anisomycin was applied to either the cell body or the dendrite compartment for 30 min, followed by glycine or vehicle application to the dendrite compartment for 3 min, and an additional 30 min of incubation in solution without glycine. Anisomycin or DMSO was present in all solutions. Then, the neurons were fixed and immunostained for surface GluN2A protein (ANOVA, Bonferroni t-tests; n = 65; * p
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    Medicago phosphate buffered saline pbs
    Serum antibody response after two doses of <t>H1-VLP</t> or split-virion vaccine. Aged (24–26 months) BALB/c mice were immunized twice with H1-VLP, split-virion vaccine or <t>PBS.</t> Three weeks post-boost sera from individual mice were analyzed by Hemagglutination Inhibition (HI) (A) and microneutralization (MN) (B) titer against A/California/07/2009 H1N1. Influenza HA-specific IgG concentrations (C) by ELISA. Dotted line in A) represents 40 HAI which is considered the protection level in humans. For statistical analysis, one-way ANOVA was used on the log values for A). For B) and C) Mann-Whitney test was performed on the log values (*** p
    Phosphate Buffered Saline Pbs, supplied by Medicago, used in various techniques. Bioz Stars score: 99/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Square wave voltammograms of a gold electrode modified by MBT/DPM–Cu (II) (solid line), after immobilization of His 6 -H1 HA monomer (dashed lines); and after filling free spaces with bovine serum albumin (finely dashed lines). Measurement conditions: 0.01 M PBS (pH 7.4), scan rate 100mVs − 1

    Journal: BMC Veterinary Research

    Article Title: Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice

    doi: 10.1186/s12917-018-1668-9

    Figure Lengend Snippet: Square wave voltammograms of a gold electrode modified by MBT/DPM–Cu (II) (solid line), after immobilization of His 6 -H1 HA monomer (dashed lines); and after filling free spaces with bovine serum albumin (finely dashed lines). Measurement conditions: 0.01 M PBS (pH 7.4), scan rate 100mVs − 1

    Article Snippet: 4-Mercapto-1-butanol (MBT), phosphate buffer saline (PBS) components (137 mM NaCl, 2.7 mM KCl, 1.8 mM Na2 HPO4 , 10 mM KH2 PO4 ), copper (II) acetate, and chloroform were obtained from Sigma-Aldrich (Poznań, Poland).

    Techniques: Modification

    The representative OSWV responses obtained for electrodes modified with Au-MBT/DPM–Cu (II)–His 6 –H1 HA monomer in the presence of: (a) sera from mice after third dose 3 × 25 μg of H1HA, (b) sera from mice after third dose of saline and adjuvant. The sera were diluted with PBS pH 7.4 in the range from (a) 0.000 (only buffer -dashed line, (b) 1 × 10 10 , (c) 5 × 10 9 , (d) 1 × 10 9 , (e) 5 × 10 8 , (f) 1 × 10 8 Measuring conditions: electrodes modified with: Au-MBT/DPM–Cu (II)–His 6 –H1 HA monomer, electrolyte: PBS buffer pH = 7.4 (buffer composition: 0.1368 M NaCl, 0.0027 M KCl, 0.0101 M Na 2 HPO 4 , 0.0018 M KH 2 PO 4 ).

    Journal: BMC Veterinary Research

    Article Title: Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice

    doi: 10.1186/s12917-018-1668-9

    Figure Lengend Snippet: The representative OSWV responses obtained for electrodes modified with Au-MBT/DPM–Cu (II)–His 6 –H1 HA monomer in the presence of: (a) sera from mice after third dose 3 × 25 μg of H1HA, (b) sera from mice after third dose of saline and adjuvant. The sera were diluted with PBS pH 7.4 in the range from (a) 0.000 (only buffer -dashed line, (b) 1 × 10 10 , (c) 5 × 10 9 , (d) 1 × 10 9 , (e) 5 × 10 8 , (f) 1 × 10 8 Measuring conditions: electrodes modified with: Au-MBT/DPM–Cu (II)–His 6 –H1 HA monomer, electrolyte: PBS buffer pH = 7.4 (buffer composition: 0.1368 M NaCl, 0.0027 M KCl, 0.0101 M Na 2 HPO 4 , 0.0018 M KH 2 PO 4 ).

    Article Snippet: 4-Mercapto-1-butanol (MBT), phosphate buffer saline (PBS) components (137 mM NaCl, 2.7 mM KCl, 1.8 mM Na2 HPO4 , 10 mM KH2 PO4 ), copper (II) acetate, and chloroform were obtained from Sigma-Aldrich (Poznań, Poland).

    Techniques: Modification, Mouse Assay

    Toxicity screening of DMSO-PBS mixtures. (a) Fluorescence micrographs of cell suspension droplets exposed to DMSO-PBS mixtures. After the exposure, the droplets were mixed with viability dye droplets to stain all cells with Hoechst and injured cells with

    Journal: Lab on a chip

    Article Title: On-chip characterization of cryoprotective agent mixtures using an EWOD-based digital microfluidic device

    doi: 10.1039/c1lc20111e

    Figure Lengend Snippet: Toxicity screening of DMSO-PBS mixtures. (a) Fluorescence micrographs of cell suspension droplets exposed to DMSO-PBS mixtures. After the exposure, the droplets were mixed with viability dye droplets to stain all cells with Hoechst and injured cells with

    Article Snippet: A 2 × 2 array of different concentrations of DMSO-PBS mixtures with or without cells was prepared by serial dilution of droplets of stock DMSO (Sigma-Aldrich, St. Louis, MO) with isotonic PBS (Invitrogen, Grand island, NY) through four basic EWOD operations - transporting, merging, mixing and separating of droplets.

    Techniques: Fluorescence, Staining

    Preparation of an array of DMSO-PBS mixtures on the EWOD chip. (a) Captured sequential images during multiplexing the mixture droplets. For visualization, the stock DMSO was dyed in red color and the boundary of PBS droplets were noted with dotted line.

    Journal: Lab on a chip

    Article Title: On-chip characterization of cryoprotective agent mixtures using an EWOD-based digital microfluidic device

    doi: 10.1039/c1lc20111e

    Figure Lengend Snippet: Preparation of an array of DMSO-PBS mixtures on the EWOD chip. (a) Captured sequential images during multiplexing the mixture droplets. For visualization, the stock DMSO was dyed in red color and the boundary of PBS droplets were noted with dotted line.

    Article Snippet: A 2 × 2 array of different concentrations of DMSO-PBS mixtures with or without cells was prepared by serial dilution of droplets of stock DMSO (Sigma-Aldrich, St. Louis, MO) with isotonic PBS (Invitrogen, Grand island, NY) through four basic EWOD operations - transporting, merging, mixing and separating of droplets.

    Techniques: Chromatin Immunoprecipitation, Multiplexing

    Phase change of DMSO-PBS mixtures on the EWOD platform. (a) Captured sequential images with temperatures during freezing and (b) thawing. The whole EWOD chip was frozen to −80°C and thawed back to room temperature at 3°C/min. The

    Journal: Lab on a chip

    Article Title: On-chip characterization of cryoprotective agent mixtures using an EWOD-based digital microfluidic device

    doi: 10.1039/c1lc20111e

    Figure Lengend Snippet: Phase change of DMSO-PBS mixtures on the EWOD platform. (a) Captured sequential images with temperatures during freezing and (b) thawing. The whole EWOD chip was frozen to −80°C and thawed back to room temperature at 3°C/min. The

    Article Snippet: A 2 × 2 array of different concentrations of DMSO-PBS mixtures with or without cells was prepared by serial dilution of droplets of stock DMSO (Sigma-Aldrich, St. Louis, MO) with isotonic PBS (Invitrogen, Grand island, NY) through four basic EWOD operations - transporting, merging, mixing and separating of droplets.

    Techniques: Chromatin Immunoprecipitation

    Screening for cryoprotection of DMSO-PBS mixtures. The cell suspension droplets were mixed with DMSO-PBS mixtures and then frozen/thawed. After F/T, these droplets were further mixed with the viability dye droplets to assess post-thaw viability. (a) Fluorescence

    Journal: Lab on a chip

    Article Title: On-chip characterization of cryoprotective agent mixtures using an EWOD-based digital microfluidic device

    doi: 10.1039/c1lc20111e

    Figure Lengend Snippet: Screening for cryoprotection of DMSO-PBS mixtures. The cell suspension droplets were mixed with DMSO-PBS mixtures and then frozen/thawed. After F/T, these droplets were further mixed with the viability dye droplets to assess post-thaw viability. (a) Fluorescence

    Article Snippet: A 2 × 2 array of different concentrations of DMSO-PBS mixtures with or without cells was prepared by serial dilution of droplets of stock DMSO (Sigma-Aldrich, St. Louis, MO) with isotonic PBS (Invitrogen, Grand island, NY) through four basic EWOD operations - transporting, merging, mixing and separating of droplets.

    Techniques: Fluorescence

    Membrane association of LidA after translocation into host cells. (A) L. pneumophila (WT) and an isogenic dotA mutant ( dotA ), both expressing GFP, were grown to postexponential phase and resuspended in PBS containing either 1% Triton X-100, 1% CHAPS,

    Journal:

    Article Title: LidA, a Translocated Substrate of the Legionella pneumophila Type IV Secretion System, Interferes with the Early Secretory Pathway

    doi: 10.1128/IAI.73.7.4370-4380.2005

    Figure Lengend Snippet: Membrane association of LidA after translocation into host cells. (A) L. pneumophila (WT) and an isogenic dotA mutant ( dotA ), both expressing GFP, were grown to postexponential phase and resuspended in PBS containing either 1% Triton X-100, 1% CHAPS,

    Article Snippet: Sixty microliters of postexponential L. pneumophila expressing green fluorescent protein (GFP) was washed once and resuspended in 5 ml of phosphate-buffered saline (PBS) containing either 1% Triton X-100 (Bio-Rad), 1% CHAPS {3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate; Sigma}, 1% octylglucoside (Calbiochem), or 1% purified digitonin (Calbiochem) and incubated for 20 min at room temperature with intermittent vortexing.

    Techniques: Translocation Assay, Mutagenesis, Expressing

    Metformin inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or PBS by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Metformin induces autophagy and G0/G1 phase cell cycle arrest in myeloma by targeting the AMPK/mTORC1 and mTORC2 pathways

    doi: 10.1186/s13046-018-0731-5

    Figure Lengend Snippet: Metformin inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or PBS by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,

    Article Snippet: Reagents Metformin (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in phosphate-buffered saline (PBS) as a stock solution of 1 M. Primary antibodies for specific detection of p-AMPK (Thr172), AMPK, Phospho-Tuberin/TSC2 (Ser1387), p-mTOR (Ser2481), p-mTOR (Ser2448), mTOR, p-p70S6K (Thr389), p70S6K, p-4EBP1 (Thr37/46), 4EBP1, p-AKT (Ser473), and AKT, as well as horseradish peroxidase (HRP)-conjugated anti-rabbit secondary detection antibodies, were purchased from Cell Signaling Technology (Beverly, MA, USA).

    Techniques: Mouse Assay, Immunohistochemistry, Expressing

    EMT with proliferation induced by EGTA with EGF+FGF-2 is caused by Wnt/β-catenin signaling by rescued by SS3Y β-catenin. (a) The TCF/LEF reporter activity was silent in cells treated by PBS, EGTA, EGF, FGF-2, TGF-β1, or EGF+FGF-2+TGF-β1 but elevated 15-fold by EGTA with EGF+FGF-2. The latter was abolished by XAV939 (*, P

    Journal: Laboratory investigation; a journal of technical methods and pathology

    Article Title: Wnt Signaling Induces Epithelial-Mesenchymal Transition with Proliferation in ARPE-19 Cells upon Loss of Contact Inhibition

    doi: 10.1038/labinvest.2011.201

    Figure Lengend Snippet: EMT with proliferation induced by EGTA with EGF+FGF-2 is caused by Wnt/β-catenin signaling by rescued by SS3Y β-catenin. (a) The TCF/LEF reporter activity was silent in cells treated by PBS, EGTA, EGF, FGF-2, TGF-β1, or EGF+FGF-2+TGF-β1 but elevated 15-fold by EGTA with EGF+FGF-2. The latter was abolished by XAV939 (*, P

    Article Snippet: Antibodies and Reagents Dulbecco’s modified Eagle’s medium (DMEM), Ham’s/F12 medium, human epidermal growth factor (EGF), HEPES buffer, phosphate-buffered saline (PBS), amphotericin B, gentamicin, fetal bovine serum (FBS), and Alexa fluor-conjugated secondary IgG were purchased from Invitrogen (Carlsbad, CA).

    Techniques: Activity Assay

    Contact inhibition is unlocked by EGTA only in the presence of EGF and/or FGF-2, but not TGF-β1. (a) Immunostaining of BrdU was performed in ARPE-19 cells cultured to day 7 post-confluence after being treated without or with 1mg/mL EGTA immediately followed by PBS, 10 ng/mL EGF, 20 ng/mL FGF-2, 10 ng/mL TGF-β1, 10 ng/mL EGF + 20 ng/mL FGF-2, or 10 ng/mL EGF + 20 ng/mL FGF-2 + 10 ng/mL TGF-β1 for 1 day. Without EGTA, no BrdU labeling was found. When EGTA was added, BrdU labeling was promoted by EGF, FGF-2, or EGF+FGF-2, but not by PBS, TGF-β1 or EGF+FGF-2+TGF-β1. Scale bar, 100 μm. (b) The BrdU labeling index was significantly increased by EGF, FGF-2, or in combination (highest) when EGTA was added (*, †, and ‡, P

    Journal: Laboratory investigation; a journal of technical methods and pathology

    Article Title: Wnt Signaling Induces Epithelial-Mesenchymal Transition with Proliferation in ARPE-19 Cells upon Loss of Contact Inhibition

    doi: 10.1038/labinvest.2011.201

    Figure Lengend Snippet: Contact inhibition is unlocked by EGTA only in the presence of EGF and/or FGF-2, but not TGF-β1. (a) Immunostaining of BrdU was performed in ARPE-19 cells cultured to day 7 post-confluence after being treated without or with 1mg/mL EGTA immediately followed by PBS, 10 ng/mL EGF, 20 ng/mL FGF-2, 10 ng/mL TGF-β1, 10 ng/mL EGF + 20 ng/mL FGF-2, or 10 ng/mL EGF + 20 ng/mL FGF-2 + 10 ng/mL TGF-β1 for 1 day. Without EGTA, no BrdU labeling was found. When EGTA was added, BrdU labeling was promoted by EGF, FGF-2, or EGF+FGF-2, but not by PBS, TGF-β1 or EGF+FGF-2+TGF-β1. Scale bar, 100 μm. (b) The BrdU labeling index was significantly increased by EGF, FGF-2, or in combination (highest) when EGTA was added (*, †, and ‡, P

    Article Snippet: Antibodies and Reagents Dulbecco’s modified Eagle’s medium (DMEM), Ham’s/F12 medium, human epidermal growth factor (EGF), HEPES buffer, phosphate-buffered saline (PBS), amphotericin B, gentamicin, fetal bovine serum (FBS), and Alexa fluor-conjugated secondary IgG were purchased from Invitrogen (Carlsbad, CA).

    Techniques: Inhibition, Immunostaining, Cell Culture, Labeling

    EGTA plus EGF+FGF-2 activates Wnt/β-catenin signaling. (a) Immunostaining revealed nuclear β-catenin and LEF1 only when cells were treated with EGTA plus EGF+FGF-2 but not EGTA alone. Scale bar, 100 μm (b) Western blot analysis confirmed increased nuclear contents of β-catenin and LEF1 in the nuclear extract, but decreased β-catenin in the membranous extract when cells were treated by EGTA with EGF+FGF-2. Levels of connexin 43, α-tubulin, and histone were used as loading controls for membranous, cytosolic and nuclear extracts, respectively. (c) Overexpression of S33Y β-catenin in post-confluent ARPE treated with EGTA without growth factors increased BrdU labeling, nuclear β-catenin and S100A4, and cytoplasmic α-SMA. (d) Immunoprecipitation by anti-β-catenin antibody followed by Western blotting with anti-LEF-1 antibody confirmed tight association between β-catenin and LEF-1 in the nuclear extract from EGTA plus EGF+FGF-2 but not from PBS or EGTA alone.

    Journal: Laboratory investigation; a journal of technical methods and pathology

    Article Title: Wnt Signaling Induces Epithelial-Mesenchymal Transition with Proliferation in ARPE-19 Cells upon Loss of Contact Inhibition

    doi: 10.1038/labinvest.2011.201

    Figure Lengend Snippet: EGTA plus EGF+FGF-2 activates Wnt/β-catenin signaling. (a) Immunostaining revealed nuclear β-catenin and LEF1 only when cells were treated with EGTA plus EGF+FGF-2 but not EGTA alone. Scale bar, 100 μm (b) Western blot analysis confirmed increased nuclear contents of β-catenin and LEF1 in the nuclear extract, but decreased β-catenin in the membranous extract when cells were treated by EGTA with EGF+FGF-2. Levels of connexin 43, α-tubulin, and histone were used as loading controls for membranous, cytosolic and nuclear extracts, respectively. (c) Overexpression of S33Y β-catenin in post-confluent ARPE treated with EGTA without growth factors increased BrdU labeling, nuclear β-catenin and S100A4, and cytoplasmic α-SMA. (d) Immunoprecipitation by anti-β-catenin antibody followed by Western blotting with anti-LEF-1 antibody confirmed tight association between β-catenin and LEF-1 in the nuclear extract from EGTA plus EGF+FGF-2 but not from PBS or EGTA alone.

    Article Snippet: Antibodies and Reagents Dulbecco’s modified Eagle’s medium (DMEM), Ham’s/F12 medium, human epidermal growth factor (EGF), HEPES buffer, phosphate-buffered saline (PBS), amphotericin B, gentamicin, fetal bovine serum (FBS), and Alexa fluor-conjugated secondary IgG were purchased from Invitrogen (Carlsbad, CA).

    Techniques: Immunostaining, Western Blot, Over Expression, Labeling, Immunoprecipitation

    ZD55-IL-18 and DTIC increased apoptosis of A375 cells. Notes: ( A ) At 48 hours after treatment, apoptotic cells were detected by the condensation of nuclear chromatin and its fragmentation. Magnification ×400. ( B ) Quantitative analysis of apoptotic A375 cells in different treatment groups. Scale bar 10 µm. Abbreviations: DTIC, dacarbazine; PBS, phosphate-buffered saline; IL-18, interleukin-18.

    Journal: Drug Design, Development and Therapy

    Article Title: Oncolytic adenovirus expressing interleukin-18 improves antitumor activity of dacarbazine for malignant melanoma

    doi: 10.2147/DDDT.S115121

    Figure Lengend Snippet: ZD55-IL-18 and DTIC increased apoptosis of A375 cells. Notes: ( A ) At 48 hours after treatment, apoptotic cells were detected by the condensation of nuclear chromatin and its fragmentation. Magnification ×400. ( B ) Quantitative analysis of apoptotic A375 cells in different treatment groups. Scale bar 10 µm. Abbreviations: DTIC, dacarbazine; PBS, phosphate-buffered saline; IL-18, interleukin-18.

    Article Snippet: Apoptosis assay Cells were washed with ice-cold phosphate-buffered saline (PBS) and incubated with annexin V-FITC and propidium iodide (BioVision, Milpitas, CA, USA) at room temperature for 10 minutes in the dark.

    Techniques:

    CAP256 gp140-FL-IP-His protein α-Env ELISA. Binding ELISAs using anti-Env human monoclonal antibodies to CAP256 gp140-FL-IP-His. (A) PGT128 and PGT135 binding indicate the presence of the Env V3-glycan supersite. Similarly, the CD4 binding site using VRC01 The V2-glycan epitope was detected with PG9 (A), but no binding was observed for the native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08 (B). In line with this, clear ELISA signals were observed for F105 and 446-52D, indicative of the presence of misfolded Env (A). (C) Control soluble, trimeric BG505_664-His Env protein performed as expected, with ELISA signals for native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08. (D) No binding of MAbs was observed for the no protein (PBS) control.

    Journal: PLoS ONE

    Article Title: The adjuvant AlhydroGel elicits higher antibody titres than AddaVax when combined with HIV-1 subtype C gp140 from CAP256

    doi: 10.1371/journal.pone.0208310

    Figure Lengend Snippet: CAP256 gp140-FL-IP-His protein α-Env ELISA. Binding ELISAs using anti-Env human monoclonal antibodies to CAP256 gp140-FL-IP-His. (A) PGT128 and PGT135 binding indicate the presence of the Env V3-glycan supersite. Similarly, the CD4 binding site using VRC01 The V2-glycan epitope was detected with PG9 (A), but no binding was observed for the native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08 (B). In line with this, clear ELISA signals were observed for F105 and 446-52D, indicative of the presence of misfolded Env (A). (C) Control soluble, trimeric BG505_664-His Env protein performed as expected, with ELISA signals for native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08. (D) No binding of MAbs was observed for the no protein (PBS) control.

    Article Snippet: ELISA plates were washed with PBS (Lonza, Basel) and blocked using 5% non-fat milk (Sigma, St Louis) in PBS.

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

    Evaluation of cellular toxicity and viability in cisplatin-treated HK-2 cells. HK-2 cells were treated with increasing concentrations of cisplatin (6.25–100 μM). (A) HK-2 cell proliferation was assessed by MTS assay after 6, 12, and 24 h of cisplatin treatment. (B) Cells were treated with the indicated concentrations of cisplatin for 24 h and cell death was measured. (C and D) HK-2 cells were washed twice with cold phosphate-buffered saline (PBS), stained with Annexin V/propidium iodide (PI), and cell death was determined by flow cytometry. (E and F) Cisplatin-induced cell viability and cytotoxicity assay using calcein AM and ethidium homodimer (EthD-1) fluorescence staining with confocal microscopy (E) and flow cytometry (F). Magnification of the images was ×40 and scale bars represent 75 μm. The mean ± S.D. (standard deviation) of three independent experiments are presented; * p

    Journal: PLoS ONE

    Article Title: Mechanism of Cisplatin-Induced Cytotoxicity Is Correlated to Impaired Metabolism Due to Mitochondrial ROS Generation

    doi: 10.1371/journal.pone.0135083

    Figure Lengend Snippet: Evaluation of cellular toxicity and viability in cisplatin-treated HK-2 cells. HK-2 cells were treated with increasing concentrations of cisplatin (6.25–100 μM). (A) HK-2 cell proliferation was assessed by MTS assay after 6, 12, and 24 h of cisplatin treatment. (B) Cells were treated with the indicated concentrations of cisplatin for 24 h and cell death was measured. (C and D) HK-2 cells were washed twice with cold phosphate-buffered saline (PBS), stained with Annexin V/propidium iodide (PI), and cell death was determined by flow cytometry. (E and F) Cisplatin-induced cell viability and cytotoxicity assay using calcein AM and ethidium homodimer (EthD-1) fluorescence staining with confocal microscopy (E) and flow cytometry (F). Magnification of the images was ×40 and scale bars represent 75 μm. The mean ± S.D. (standard deviation) of three independent experiments are presented; * p

    Article Snippet: Finally, the cells were diluted in 200 μL of phosphate-buffered saline (PBS) for analysis by flow cytometry (FACSAria III, BD Biosciences).

    Techniques: MTS Assay, Staining, Flow Cytometry, Cytometry, Cytotoxicity Assay, Ethidium Homodimer Assay, Fluorescence, Confocal Microscopy, Standard Deviation

    Effect of diosgenin on SHh, SMO and GLI1 gene expression during megakaryocytic differentiation in HEL and TF1a cell lines. (A) Cells were treated or not with 10 µM diosgenin for 24, 48, 72 and 96 h then SHh, SMO and GLI1 genes expressions were evaluated. Total RNA was extracted and 2µg of total RNA were transcribed into cDNA and used for PCR. PCR resulting fragments were visualized by electrophoresis on a 1% agarose gel containing ethidium bromide. Quantification of SHh, SMO and GLI1 transcripts were normalized to 18S as an internal control. The agarose gels shown are representative of six separate experiments. (B) Cells were treated or not with 10µM diosgenin for 96 h and megakaryocytic differentiation was evaluated by analyzing nuclear ploidy. Cells were fixed and permeabilized in 70% ethanol in PBS at −20 °C overnight, washed in PBS, treated with RNase and stained with PI. Then, flow cytometric analyses (FC) were performed to analyze DNA content.

    Journal: PLoS ONE

    Article Title: Sonic Hedgehog Activation Is Implicated in Diosgenin-Induced Megakaryocytic Differentiation of Human Erythroleukemia Cells

    doi: 10.1371/journal.pone.0095016

    Figure Lengend Snippet: Effect of diosgenin on SHh, SMO and GLI1 gene expression during megakaryocytic differentiation in HEL and TF1a cell lines. (A) Cells were treated or not with 10 µM diosgenin for 24, 48, 72 and 96 h then SHh, SMO and GLI1 genes expressions were evaluated. Total RNA was extracted and 2µg of total RNA were transcribed into cDNA and used for PCR. PCR resulting fragments were visualized by electrophoresis on a 1% agarose gel containing ethidium bromide. Quantification of SHh, SMO and GLI1 transcripts were normalized to 18S as an internal control. The agarose gels shown are representative of six separate experiments. (B) Cells were treated or not with 10µM diosgenin for 96 h and megakaryocytic differentiation was evaluated by analyzing nuclear ploidy. Cells were fixed and permeabilized in 70% ethanol in PBS at −20 °C overnight, washed in PBS, treated with RNase and stained with PI. Then, flow cytometric analyses (FC) were performed to analyze DNA content.

    Article Snippet: Evaluation of nuclear ploidy For DNA content analysis, after treatment, cells were fixed and permeabilized in 70% ethanol in phosphate-buffered saline (PBS) at −20 °C overnight, washed in PBS, treated with RNase (40 U/μl, Boehringer Mannheim, Meylan, France) for 1 h at room temperature and stained with propidium iodide (PI) (50 µg/ml).

    Techniques: Expressing, Polymerase Chain Reaction, Electrophoresis, Agarose Gel Electrophoresis, Staining, Flow Cytometry

    Survival of C3-deficient mice challenged i.n. with WU2 after i.p. administration of PPS3-binding MAbs. (A) C3 KO mice. Survival of C3 KO mice that received 10 μg 1E2, 5F6, or 7A9 was significantly longer than that of PBS-treated animals (*,

    Journal: Infection and Immunity

    Article Title: Efficacy of Opsonic and Nonopsonic Serotype 3 Pneumococcal Capsular Polysaccharide-Specific Monoclonal Antibodies against Intranasal Challenge with Streptococcus pneumoniae in Mice ▿

    doi: 10.1128/IAI.01075-08

    Figure Lengend Snippet: Survival of C3-deficient mice challenged i.n. with WU2 after i.p. administration of PPS3-binding MAbs. (A) C3 KO mice. Survival of C3 KO mice that received 10 μg 1E2, 5F6, or 7A9 was significantly longer than that of PBS-treated animals (*,

    Article Snippet: Briefly, 96-well polystyrene ELISA plates (Corning Glass Works, Corning, NY) were coated with 10 μg/ml PPS3 (6303) in phosphate-buffered saline (PBS) (Cambrex, Walkersville, MD) for 3 h at room temperature, followed by blocking with 1% bovine serum albumin (Sigma-Aldrich, St. Louis, MO)-PBS overnight at 4°C.

    Techniques: Mouse Assay, Binding Assay

    Survival of FcγR-deficient mice challenged i.n. with WU2 after i.p. administration of PPS3-binding MAbs. (A) FcγR KO mice. Survival of FcγR KO mice that received 5F6 and 7A9 was significantly longer than that of PBS-treated animals

    Journal: Infection and Immunity

    Article Title: Efficacy of Opsonic and Nonopsonic Serotype 3 Pneumococcal Capsular Polysaccharide-Specific Monoclonal Antibodies against Intranasal Challenge with Streptococcus pneumoniae in Mice ▿

    doi: 10.1128/IAI.01075-08

    Figure Lengend Snippet: Survival of FcγR-deficient mice challenged i.n. with WU2 after i.p. administration of PPS3-binding MAbs. (A) FcγR KO mice. Survival of FcγR KO mice that received 5F6 and 7A9 was significantly longer than that of PBS-treated animals

    Article Snippet: Briefly, 96-well polystyrene ELISA plates (Corning Glass Works, Corning, NY) were coated with 10 μg/ml PPS3 (6303) in phosphate-buffered saline (PBS) (Cambrex, Walkersville, MD) for 3 h at room temperature, followed by blocking with 1% bovine serum albumin (Sigma-Aldrich, St. Louis, MO)-PBS overnight at 4°C.

    Techniques: Mouse Assay, Binding Assay

    Visualization of the fluorescence intensitites of the microarrays. The upper part shows a subset of the 269 control DBSS. The Swedish C3 deficient and C3 positive serum standards contains 23 serial dilutions each (high to low dilution, 1∶5 to 1∶100 000). The two lowest dilutions of the standard curve are separated by a blank (PBS). The dotted rectangle depicts the C3 deficient sample.

    Journal: PLoS ONE

    Article Title: Screening for C3 Deficiency in Newborns Using Microarrays

    doi: 10.1371/journal.pone.0005321

    Figure Lengend Snippet: Visualization of the fluorescence intensitites of the microarrays. The upper part shows a subset of the 269 control DBSS. The Swedish C3 deficient and C3 positive serum standards contains 23 serial dilutions each (high to low dilution, 1∶5 to 1∶100 000). The two lowest dilutions of the standard curve are separated by a blank (PBS). The dotted rectangle depicts the C3 deficient sample.

    Article Snippet: For determination of C3 levels by microarrays, the serum samples were diluted 1∶100 in phosphate-buffered saline (PBS) with 0.5% Tween20 and printed onto Epoxide slides (Corning, the Netherlands) using a non-contact printing robot (Nano-plotter 2.0, Gesim, Germany), which deposits approximately 0.4 nl/drop.

    Techniques: Fluorescence

    MTT Assay: A. Without Light: 1-AP exposed HaCaT cells remained in dark conditions for 20 min in 1× PBS buffer. After treatment, DMEM was added to the cells, and they underwent incubation at 37°C/ 5 %CO 2 for 0, 2, 4, 6, and 24 hrs (N=3).

    Journal: Toxicology research

    Article Title: DNA damage and repair of human skin keratinocytes concurrently exposed to pyrene derivatives and UVA light

    doi: 10.1039/C3TX20085J

    Figure Lengend Snippet: MTT Assay: A. Without Light: 1-AP exposed HaCaT cells remained in dark conditions for 20 min in 1× PBS buffer. After treatment, DMEM was added to the cells, and they underwent incubation at 37°C/ 5 %CO 2 for 0, 2, 4, 6, and 24 hrs (N=3).

    Article Snippet: Fetal Bovine Serum (FBS), Dulbecco’s minimum essential medium (DMEM), Penicillin/streptomycin, methanol and phosphate buffered saline (PBS) were from Fisher Scientific (Houston, TX).

    Techniques: MTT Assay, Incubation

    Formation of granulomas are necessary for the increased IL-1RA production within the liver of CFZ-treated mice. A) Liver cryosections of PBS or clodronate treated mice fed a control diet or CFZ-supplemented diet stained for F4/80 (green) and showing CLDI fluorescence. Treatment with clodronate reduces hepatic macrophages, resulting in reduced granuloma size and CLDI accumulation within the granuloma. B) IL-1RA production is inhibited within clodronate-CFZ treated mice, while it is significantly elevated in the PBS-CFZ treatment group. C) The granulomas which form ultimately are responsible for the production of IL-1RA, producing similar levels of IL-1RA to whole-organ homogenates. Scale bar is 50 um. (n=3-4 liver homogenates or isolated granulomas per treatment group) (*=p

    Journal: Pharmaceutical research

    Article Title: An Expandable Mechanopharmaceutical Device (2): Drug Induced Granulomas Maximize the Cargo Sequestering Capacity of Macrophages in the Liver

    doi: 10.1007/s11095-018-2541-z

    Figure Lengend Snippet: Formation of granulomas are necessary for the increased IL-1RA production within the liver of CFZ-treated mice. A) Liver cryosections of PBS or clodronate treated mice fed a control diet or CFZ-supplemented diet stained for F4/80 (green) and showing CLDI fluorescence. Treatment with clodronate reduces hepatic macrophages, resulting in reduced granuloma size and CLDI accumulation within the granuloma. B) IL-1RA production is inhibited within clodronate-CFZ treated mice, while it is significantly elevated in the PBS-CFZ treatment group. C) The granulomas which form ultimately are responsible for the production of IL-1RA, producing similar levels of IL-1RA to whole-organ homogenates. Scale bar is 50 um. (n=3-4 liver homogenates or isolated granulomas per treatment group) (*=p

    Article Snippet: To deplete tissue macrophages, mice (n=3–4 per treatment group) were treated with liposomes containing either 7 mg/mL of clodronate or phosphate-buffered saline (PBS) (FormuMax Scientific Inc., Sunnyvale, CA).

    Techniques: Mouse Assay, Staining, Fluorescence, Isolation

    Effects of U0126 or LY294002 or the combination of U1026 and LY294002 on severe combined immunodeficiency (SCID) mice bearing EHMES-10 cells. (A–C) Survival times of EHMES-10 cell-bearing SCID mice treated with U0126, LY294002, or the combination of U0126 and LY294002. EHMES-10 cells (3×10 6 ) were inoculated into the thoracic cavity of SCID mice. Seven days after inoculation, SCID mice were randomized into eight groups (n=7 mice/group) to receive vehicle (DMSO + PBS), U0126 (20, 30 and 40 mg/kg), or LY294002 (12.5, 25 and 50 mg/kg), or a combination of U0126 (30 mg/kg) and LY294002 (25 mg/kg). U, U0126; LY, LY294002; * P

    Journal: International Journal of Oncology

    Article Title: Antitumor activity of MEK and PI3K inhibitors against malignant pleural mesothelioma cells in vitro and in vivo

    doi: 10.3892/ijo.2012.1462

    Figure Lengend Snippet: Effects of U0126 or LY294002 or the combination of U1026 and LY294002 on severe combined immunodeficiency (SCID) mice bearing EHMES-10 cells. (A–C) Survival times of EHMES-10 cell-bearing SCID mice treated with U0126, LY294002, or the combination of U0126 and LY294002. EHMES-10 cells (3×10 6 ) were inoculated into the thoracic cavity of SCID mice. Seven days after inoculation, SCID mice were randomized into eight groups (n=7 mice/group) to receive vehicle (DMSO + PBS), U0126 (20, 30 and 40 mg/kg), or LY294002 (12.5, 25 and 50 mg/kg), or a combination of U0126 (30 mg/kg) and LY294002 (25 mg/kg). U, U0126; LY, LY294002; * P

    Article Snippet: For in vivo studies, these agents were prepared as a suspension in a vehicle consisting of 40% DMSO in phosphate-buffered saline (PBS) (Wako Pure Chemical Industries, Osaka, Japan).

    Techniques: Mouse Assay

    Dendritic protein synthesis is required for glycine-induced insertion of GluN2A ( A ) MAP2 staining of 25 DIV neurons cultured in a microfluidic device (CB: cell bodies, D: dendrites). Scale bar is 20 μm. ( B ) A representative dendritic region immunostained for MAP2 and GluN2A. Scale bar is 3 μm. ( C ) Time-lapse imaging shows microfluidic chambers maintain fluidic isolation throughout the glycine treatment paradigm. Microfluidic devices were filled with PBS (initial), then left-side PBS was replaced with PBS plus Cy3b dye (t 0 ), and, 30 min later, right-side PBS was replaced with PBS plus Cy5 dye (t 30 ). After 3 min, PBS plus Cy5 dye was replaced with PBS alone (t 33 ) and allowed to sit for an additional 30 min (t 60 ). The Cy3 and Cy5 dye solutions remained restricted to the cell body (CB) and dendrite (sides), respectively, for the duration of the experiment. ( D ) Hippocampal neurons were cultured in microfluidic devices. Anisomycin was applied to either the cell body or the dendrite compartment for 30 min, followed by glycine or vehicle application to the dendrite compartment for 3 min, and an additional 30 min of incubation in solution without glycine. Anisomycin or DMSO was present in all solutions. Then, the neurons were fixed and immunostained for surface GluN2A protein (ANOVA, Bonferroni t-tests; n = 65; * p

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Dendritic GluN2A synthesis mediates activity-induced NMDA receptor insertion

    doi: 10.1523/JNEUROSCI.0289-13.2013

    Figure Lengend Snippet: Dendritic protein synthesis is required for glycine-induced insertion of GluN2A ( A ) MAP2 staining of 25 DIV neurons cultured in a microfluidic device (CB: cell bodies, D: dendrites). Scale bar is 20 μm. ( B ) A representative dendritic region immunostained for MAP2 and GluN2A. Scale bar is 3 μm. ( C ) Time-lapse imaging shows microfluidic chambers maintain fluidic isolation throughout the glycine treatment paradigm. Microfluidic devices were filled with PBS (initial), then left-side PBS was replaced with PBS plus Cy3b dye (t 0 ), and, 30 min later, right-side PBS was replaced with PBS plus Cy5 dye (t 30 ). After 3 min, PBS plus Cy5 dye was replaced with PBS alone (t 33 ) and allowed to sit for an additional 30 min (t 60 ). The Cy3 and Cy5 dye solutions remained restricted to the cell body (CB) and dendrite (sides), respectively, for the duration of the experiment. ( D ) Hippocampal neurons were cultured in microfluidic devices. Anisomycin was applied to either the cell body or the dendrite compartment for 30 min, followed by glycine or vehicle application to the dendrite compartment for 3 min, and an additional 30 min of incubation in solution without glycine. Anisomycin or DMSO was present in all solutions. Then, the neurons were fixed and immunostained for surface GluN2A protein (ANOVA, Bonferroni t-tests; n = 65; * p

    Article Snippet: In anisomycin experiments, anisomycin- or DMSO-containing solution was added to the cell body or dendrite side for 30 min. For glycine stimulation, the extracellular solution was removed and glycine-containing solution (or control) was added to the dendrite side for 3 min, then replaced with extracellular solution (without glycine) for 30 min. To ensure that microfluidic isolation was maintained during this treatment protocol, we used time-lapse imaging and phosphate-buffered saline (PBS) containing Cy3 or Cy5 dye (GE Healthcare) to visualize a mock glycine-induced LTP paradigm in the microfluidic device ( ).

    Techniques: Staining, Cell Culture, Imaging, Isolation, Incubation

    Serum antibody response after two doses of H1-VLP or split-virion vaccine. Aged (24–26 months) BALB/c mice were immunized twice with H1-VLP, split-virion vaccine or PBS. Three weeks post-boost sera from individual mice were analyzed by Hemagglutination Inhibition (HI) (A) and microneutralization (MN) (B) titer against A/California/07/2009 H1N1. Influenza HA-specific IgG concentrations (C) by ELISA. Dotted line in A) represents 40 HAI which is considered the protection level in humans. For statistical analysis, one-way ANOVA was used on the log values for A). For B) and C) Mann-Whitney test was performed on the log values (*** p

    Journal: PLoS ONE

    Article Title: A plant-derived VLP influenza vaccine elicits a balanced immune response even in very old mice with co-morbidities

    doi: 10.1371/journal.pone.0210009

    Figure Lengend Snippet: Serum antibody response after two doses of H1-VLP or split-virion vaccine. Aged (24–26 months) BALB/c mice were immunized twice with H1-VLP, split-virion vaccine or PBS. Three weeks post-boost sera from individual mice were analyzed by Hemagglutination Inhibition (HI) (A) and microneutralization (MN) (B) titer against A/California/07/2009 H1N1. Influenza HA-specific IgG concentrations (C) by ELISA. Dotted line in A) represents 40 HAI which is considered the protection level in humans. For statistical analysis, one-way ANOVA was used on the log values for A). For B) and C) Mann-Whitney test was performed on the log values (*** p

    Article Snippet: Virus, mice and vaccines Female BALB/c mice (Charles River Laboratories, Montreal, QC) near the end of their life-spans (24–26 months age) were vaccinated with two doses of phosphate buffered saline (PBS), H1-VLP vaccine (Medicago, Quebec City, QC) or split inactivated H1N1 vaccine (BEI resources, Manassas, VA).

    Techniques: Mouse Assay, HI Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Cytokine production by splenocytes after ex vivo stimulation three weeks post-boost. Aged (24–26 months) BALB/c mice were immunized twice with H1-VLP, split-virion vaccine or PBS. Three weeks post-boost, splenocytes were collected and stimulated ex vivo for 18 hours with H1-VLP. Flow cytometry was performed to determine the following cell types and cytokine expression: A) CD4 + T cells and B) CD8 + T cells. Subtractive data was used (Stimulated—unstimulated). For statistical analysis, two-way ANOVA was performed (**** p

    Journal: PLoS ONE

    Article Title: A plant-derived VLP influenza vaccine elicits a balanced immune response even in very old mice with co-morbidities

    doi: 10.1371/journal.pone.0210009

    Figure Lengend Snippet: Cytokine production by splenocytes after ex vivo stimulation three weeks post-boost. Aged (24–26 months) BALB/c mice were immunized twice with H1-VLP, split-virion vaccine or PBS. Three weeks post-boost, splenocytes were collected and stimulated ex vivo for 18 hours with H1-VLP. Flow cytometry was performed to determine the following cell types and cytokine expression: A) CD4 + T cells and B) CD8 + T cells. Subtractive data was used (Stimulated—unstimulated). For statistical analysis, two-way ANOVA was performed (**** p

    Article Snippet: Virus, mice and vaccines Female BALB/c mice (Charles River Laboratories, Montreal, QC) near the end of their life-spans (24–26 months age) were vaccinated with two doses of phosphate buffered saline (PBS), H1-VLP vaccine (Medicago, Quebec City, QC) or split inactivated H1N1 vaccine (BEI resources, Manassas, VA).

    Techniques: Ex Vivo, Mouse Assay, Flow Cytometry, Cytometry, Expressing