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  • 92
    Stratagene pbluescript ii sk
    DNase I footprinting analyses of protein-DNA interactions on the DS fragment. A , pKS-AHF contains a 251-bp sequence (HinfI site at 8945 nt; AluI site at 9195 nt of the EBV B95-8 genome) carrying DS on the <t>pBluescript</t> vector. B , a 0.44-kbp DNA of pKS-AHF
    Pbluescript Ii Sk, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 5528 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies pbluescript ii sk
    Constructions of recombinant WHV-HBV genomes and transient transfection of recombinant WHV-HBV genomes in hepatoma cells. (A) The schematic map of recombinant WHV-HBV genomes. pBS-WHV1.3 contained a 1.3 fold overlength WHV genome in <t>pBluescript</t> II SK(+) vector and was used as backbone. The respective WHV genome regions were replaced by the corresponding HBV sequences, shown as black bars. The plasmids pWHV-HBV-Sa, pWHV-HBV-SS, and pWHV-HBV-MS contained HBV sequences encoding HBsAg a-determinant only, major HBsAg and middle HBsAg, respectively. (B) HBsAg expression in the supernatant of the transfected hepatoma cells. Huh7 and HepG2 cells were transiently transfected with plasmids of pBS-WHV1.3 (W1.3), pWHV-HBV-Sa (Sa), pWHV-HBV-SS (SS), pWHV-HBV-MS (MS), pBS-HBV1.3 (H1.3), and pHBc (HBc). The culture supernatants were collected at 24, 48, 72, and 96 hours after transfection for the detection of HBsAg by ELISA. The results were read at OD 450 nm. The cut off value was set as 0.1 and indicated by the dotted line.
    Pbluescript Ii Sk, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pbluescript ii sk
    Electrophoretic mobility shift assay using the PinifC3 promoter. Labeled double-stranded DNA from the −151 to −92 region of PinifC3 was mixed with 10 μg of nuclear protein from cleaving sporangia and increasing amounts of unlabeled competitor DNA representing an approximately 80- to 1,280-fold excess. The competitors are, left to right, the normal −151 to −92 region (wild type); the −151 to −92 region containing an altered 7-nt cold box (mutated CB); and the <t>pBluescript</t> II SK(+) multiple cloning site (nonspecific). The graphs below the gel show quantitations of bands a and b (left and right panels, respectively), using increasing excess wild-type DNA competitor (circles), cold-box-mutated competitor (squares), and nonspecific competitor (diamonds).
    Pbluescript Ii Sk, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Toyobo pbluescript ii sk
    SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and GFC analysis of PAP. (A) SDS-PAGE analysis. A gradient gel (8 to 16% PAGE) was used for SDS-PAGE. Lanes: M, molecular mass markers; C, whole-cell extract of E. coli harboring <t>pBluescript</t> SK(+)
    Pbluescript Ii Sk, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc pbluescript ii sk
    SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and GFC analysis of PAP. (A) SDS-PAGE analysis. A gradient gel (8 to 16% PAGE) was used for SDS-PAGE. Lanes: M, molecular mass markers; C, whole-cell extract of E. coli harboring <t>pBluescript</t> SK(+)
    Pbluescript Ii Sk, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore pbluescript ii sk
    SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and GFC analysis of PAP. (A) SDS-PAGE analysis. A gradient gel (8 to 16% PAGE) was used for SDS-PAGE. Lanes: M, molecular mass markers; C, whole-cell extract of E. coli harboring <t>pBluescript</t> SK(+)
    Pbluescript Ii Sk, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad pbluescript ii sk
    SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and GFC analysis of PAP. (A) SDS-PAGE analysis. A gradient gel (8 to 16% PAGE) was used for SDS-PAGE. Lanes: M, molecular mass markers; C, whole-cell extract of E. coli harboring <t>pBluescript</t> SK(+)
    Pbluescript Ii Sk, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pbluescript sk ii
    SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and GFC analysis of PAP. (A) SDS-PAGE analysis. A gradient gel (8 to 16% PAGE) was used for SDS-PAGE. Lanes: M, molecular mass markers; C, whole-cell extract of E. coli harboring <t>pBluescript</t> SK(+)
    Pbluescript Sk Ii, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pbluescript ii sk
    Effects of constitutively active calcineurin and constitutively active PKA on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for VAMP2-EGFP (top), for caCN and VAMP2-EGFP (middle), and for PKA * and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, <t>pBluescript</t> II SK+. B , A single axon terminal of an olfactory sensory neuron in a zebrafish embryo injected with omp promoter-driven VAMP2-EGFP and Bassoon-EYFP vectors at 50 hpf. VAMP2-ECFP (left; green) and Bassoon-EYFP (middle; red) signals were merged on the right. Arrowheads point to axonal shafts. Scale bar, 5 μm. C , Confocal fluorescent images of immunostaining with anti-GFP (left; green) and anti-syntaxin (middle; red) antibodies in the olfactory bulb from an omp promoter-driven VAMP2-ECFP transgenic fish embryo at 60 hpf. Merged image is on the right. Scale bar, 10 μm. D-F , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in Pomp-VG-injected embryos at 36 hpf ( D ), 60 hpf ( E ), and 84 hpf ( F ). Scale bar, 5 μm. G-I , The threshold images of VAMP2-EGFP signals in D-F for evaluation of VAMP2-EGFP puncta. J-O , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG-caCN ( J-L ) or Pomp-VG-PKA * ( M-O ) at 36 hpf ( J , M ), 60 hpf ( K , N ), and 84 hpf ( L , O ). Arrowheads point to axonal shafts. P , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-caCN (black bars), and Pomp-VG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 37, 26, and 30 at 36, 60, and 84 hpf, respectively, 21-23 embryos; Pomp-VG-caCN, n = 40, 34, and 34 at 36, 60, and 84 hpf, respectively, 24-26 embryos; Pomp-VG-PKA * , n = 25, 37, and 39 at 36, 60, and 84 hpf, respectively, 20-22 embryos. All values represent mean ± SEM. ** p
    Pbluescript Ii Sk, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies pbluescritpt ii sk
    Effects of constitutively active calcineurin and constitutively active PKA on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for VAMP2-EGFP (top), for caCN and VAMP2-EGFP (middle), and for PKA * and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, <t>pBluescript</t> II SK+. B , A single axon terminal of an olfactory sensory neuron in a zebrafish embryo injected with omp promoter-driven VAMP2-EGFP and Bassoon-EYFP vectors at 50 hpf. VAMP2-ECFP (left; green) and Bassoon-EYFP (middle; red) signals were merged on the right. Arrowheads point to axonal shafts. Scale bar, 5 μm. C , Confocal fluorescent images of immunostaining with anti-GFP (left; green) and anti-syntaxin (middle; red) antibodies in the olfactory bulb from an omp promoter-driven VAMP2-ECFP transgenic fish embryo at 60 hpf. Merged image is on the right. Scale bar, 10 μm. D-F , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in Pomp-VG-injected embryos at 36 hpf ( D ), 60 hpf ( E ), and 84 hpf ( F ). Scale bar, 5 μm. G-I , The threshold images of VAMP2-EGFP signals in D-F for evaluation of VAMP2-EGFP puncta. J-O , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG-caCN ( J-L ) or Pomp-VG-PKA * ( M-O ) at 36 hpf ( J , M ), 60 hpf ( K , N ), and 84 hpf ( L , O ). Arrowheads point to axonal shafts. P , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-caCN (black bars), and Pomp-VG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 37, 26, and 30 at 36, 60, and 84 hpf, respectively, 21-23 embryos; Pomp-VG-caCN, n = 40, 34, and 34 at 36, 60, and 84 hpf, respectively, 24-26 embryos; Pomp-VG-PKA * , n = 25, 37, and 39 at 36, 60, and 84 hpf, respectively, 20-22 embryos. All values represent mean ± SEM. ** p
    Pbluescritpt Ii Sk, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene pbluscript ii sk
    Effects of constitutively active calcineurin and constitutively active PKA on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for VAMP2-EGFP (top), for caCN and VAMP2-EGFP (middle), and for PKA * and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, <t>pBluescript</t> II SK+. B , A single axon terminal of an olfactory sensory neuron in a zebrafish embryo injected with omp promoter-driven VAMP2-EGFP and Bassoon-EYFP vectors at 50 hpf. VAMP2-ECFP (left; green) and Bassoon-EYFP (middle; red) signals were merged on the right. Arrowheads point to axonal shafts. Scale bar, 5 μm. C , Confocal fluorescent images of immunostaining with anti-GFP (left; green) and anti-syntaxin (middle; red) antibodies in the olfactory bulb from an omp promoter-driven VAMP2-ECFP transgenic fish embryo at 60 hpf. Merged image is on the right. Scale bar, 10 μm. D-F , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in Pomp-VG-injected embryos at 36 hpf ( D ), 60 hpf ( E ), and 84 hpf ( F ). Scale bar, 5 μm. G-I , The threshold images of VAMP2-EGFP signals in D-F for evaluation of VAMP2-EGFP puncta. J-O , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG-caCN ( J-L ) or Pomp-VG-PKA * ( M-O ) at 36 hpf ( J , M ), 60 hpf ( K , N ), and 84 hpf ( L , O ). Arrowheads point to axonal shafts. P , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-caCN (black bars), and Pomp-VG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 37, 26, and 30 at 36, 60, and 84 hpf, respectively, 21-23 embryos; Pomp-VG-caCN, n = 40, 34, and 34 at 36, 60, and 84 hpf, respectively, 24-26 embryos; Pomp-VG-PKA * , n = 25, 37, and 39 at 36, 60, and 84 hpf, respectively, 20-22 embryos. All values represent mean ± SEM. ** p
    Pbluscript Ii Sk, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene pbluescript ii sk psk
    Effects of constitutively active calcineurin and constitutively active PKA on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for VAMP2-EGFP (top), for caCN and VAMP2-EGFP (middle), and for PKA * and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, <t>pBluescript</t> II SK+. B , A single axon terminal of an olfactory sensory neuron in a zebrafish embryo injected with omp promoter-driven VAMP2-EGFP and Bassoon-EYFP vectors at 50 hpf. VAMP2-ECFP (left; green) and Bassoon-EYFP (middle; red) signals were merged on the right. Arrowheads point to axonal shafts. Scale bar, 5 μm. C , Confocal fluorescent images of immunostaining with anti-GFP (left; green) and anti-syntaxin (middle; red) antibodies in the olfactory bulb from an omp promoter-driven VAMP2-ECFP transgenic fish embryo at 60 hpf. Merged image is on the right. Scale bar, 10 μm. D-F , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in Pomp-VG-injected embryos at 36 hpf ( D ), 60 hpf ( E ), and 84 hpf ( F ). Scale bar, 5 μm. G-I , The threshold images of VAMP2-EGFP signals in D-F for evaluation of VAMP2-EGFP puncta. J-O , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG-caCN ( J-L ) or Pomp-VG-PKA * ( M-O ) at 36 hpf ( J , M ), 60 hpf ( K , N ), and 84 hpf ( L , O ). Arrowheads point to axonal shafts. P , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-caCN (black bars), and Pomp-VG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 37, 26, and 30 at 36, 60, and 84 hpf, respectively, 21-23 embryos; Pomp-VG-caCN, n = 40, 34, and 34 at 36, 60, and 84 hpf, respectively, 24-26 embryos; Pomp-VG-PKA * , n = 25, 37, and 39 at 36, 60, and 84 hpf, respectively, 20-22 embryos. All values represent mean ± SEM. ** p
    Pbluescript Ii Sk Psk, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene pbluescript ii sk mcs
    Effects of constitutively active calcineurin and constitutively active PKA on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for VAMP2-EGFP (top), for caCN and VAMP2-EGFP (middle), and for PKA * and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, <t>pBluescript</t> II SK+. B , A single axon terminal of an olfactory sensory neuron in a zebrafish embryo injected with omp promoter-driven VAMP2-EGFP and Bassoon-EYFP vectors at 50 hpf. VAMP2-ECFP (left; green) and Bassoon-EYFP (middle; red) signals were merged on the right. Arrowheads point to axonal shafts. Scale bar, 5 μm. C , Confocal fluorescent images of immunostaining with anti-GFP (left; green) and anti-syntaxin (middle; red) antibodies in the olfactory bulb from an omp promoter-driven VAMP2-ECFP transgenic fish embryo at 60 hpf. Merged image is on the right. Scale bar, 10 μm. D-F , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in Pomp-VG-injected embryos at 36 hpf ( D ), 60 hpf ( E ), and 84 hpf ( F ). Scale bar, 5 μm. G-I , The threshold images of VAMP2-EGFP signals in D-F for evaluation of VAMP2-EGFP puncta. J-O , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG-caCN ( J-L ) or Pomp-VG-PKA * ( M-O ) at 36 hpf ( J , M ), 60 hpf ( K , N ), and 84 hpf ( L , O ). Arrowheads point to axonal shafts. P , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-caCN (black bars), and Pomp-VG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 37, 26, and 30 at 36, 60, and 84 hpf, respectively, 21-23 embryos; Pomp-VG-caCN, n = 40, 34, and 34 at 36, 60, and 84 hpf, respectively, 24-26 embryos; Pomp-VG-PKA * , n = 25, 37, and 39 at 36, 60, and 84 hpf, respectively, 20-22 embryos. All values represent mean ± SEM. ** p
    Pbluescript Ii Sk Mcs, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies pbluscript ii sk
    Effects of constitutively active calcineurin and constitutively active PKA on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for VAMP2-EGFP (top), for caCN and VAMP2-EGFP (middle), and for PKA * and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, <t>pBluescript</t> II SK+. B , A single axon terminal of an olfactory sensory neuron in a zebrafish embryo injected with omp promoter-driven VAMP2-EGFP and Bassoon-EYFP vectors at 50 hpf. VAMP2-ECFP (left; green) and Bassoon-EYFP (middle; red) signals were merged on the right. Arrowheads point to axonal shafts. Scale bar, 5 μm. C , Confocal fluorescent images of immunostaining with anti-GFP (left; green) and anti-syntaxin (middle; red) antibodies in the olfactory bulb from an omp promoter-driven VAMP2-ECFP transgenic fish embryo at 60 hpf. Merged image is on the right. Scale bar, 10 μm. D-F , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in Pomp-VG-injected embryos at 36 hpf ( D ), 60 hpf ( E ), and 84 hpf ( F ). Scale bar, 5 μm. G-I , The threshold images of VAMP2-EGFP signals in D-F for evaluation of VAMP2-EGFP puncta. J-O , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG-caCN ( J-L ) or Pomp-VG-PKA * ( M-O ) at 36 hpf ( J , M ), 60 hpf ( K , N ), and 84 hpf ( L , O ). Arrowheads point to axonal shafts. P , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-caCN (black bars), and Pomp-VG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 37, 26, and 30 at 36, 60, and 84 hpf, respectively, 21-23 embryos; Pomp-VG-caCN, n = 40, 34, and 34 at 36, 60, and 84 hpf, respectively, 24-26 embryos; Pomp-VG-PKA * , n = 25, 37, and 39 at 36, 60, and 84 hpf, respectively, 20-22 embryos. All values represent mean ± SEM. ** p
    Pbluscript Ii Sk, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene pbluescipt ii sk
    Effects of constitutively active calcineurin and constitutively active PKA on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for VAMP2-EGFP (top), for caCN and VAMP2-EGFP (middle), and for PKA * and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, <t>pBluescript</t> II SK+. B , A single axon terminal of an olfactory sensory neuron in a zebrafish embryo injected with omp promoter-driven VAMP2-EGFP and Bassoon-EYFP vectors at 50 hpf. VAMP2-ECFP (left; green) and Bassoon-EYFP (middle; red) signals were merged on the right. Arrowheads point to axonal shafts. Scale bar, 5 μm. C , Confocal fluorescent images of immunostaining with anti-GFP (left; green) and anti-syntaxin (middle; red) antibodies in the olfactory bulb from an omp promoter-driven VAMP2-ECFP transgenic fish embryo at 60 hpf. Merged image is on the right. Scale bar, 10 μm. D-F , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in Pomp-VG-injected embryos at 36 hpf ( D ), 60 hpf ( E ), and 84 hpf ( F ). Scale bar, 5 μm. G-I , The threshold images of VAMP2-EGFP signals in D-F for evaluation of VAMP2-EGFP puncta. J-O , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG-caCN ( J-L ) or Pomp-VG-PKA * ( M-O ) at 36 hpf ( J , M ), 60 hpf ( K , N ), and 84 hpf ( L , O ). Arrowheads point to axonal shafts. P , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-caCN (black bars), and Pomp-VG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 37, 26, and 30 at 36, 60, and 84 hpf, respectively, 21-23 embryos; Pomp-VG-caCN, n = 40, 34, and 34 at 36, 60, and 84 hpf, respectively, 24-26 embryos; Pomp-VG-PKA * , n = 25, 37, and 39 at 36, 60, and 84 hpf, respectively, 20-22 embryos. All values represent mean ± SEM. ** p
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    88
    Stratagene pbluescript ii sk phagemide
    Effects of constitutively active calcineurin and constitutively active PKA on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for VAMP2-EGFP (top), for caCN and VAMP2-EGFP (middle), and for PKA * and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, <t>pBluescript</t> II SK+. B , A single axon terminal of an olfactory sensory neuron in a zebrafish embryo injected with omp promoter-driven VAMP2-EGFP and Bassoon-EYFP vectors at 50 hpf. VAMP2-ECFP (left; green) and Bassoon-EYFP (middle; red) signals were merged on the right. Arrowheads point to axonal shafts. Scale bar, 5 μm. C , Confocal fluorescent images of immunostaining with anti-GFP (left; green) and anti-syntaxin (middle; red) antibodies in the olfactory bulb from an omp promoter-driven VAMP2-ECFP transgenic fish embryo at 60 hpf. Merged image is on the right. Scale bar, 10 μm. D-F , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in Pomp-VG-injected embryos at 36 hpf ( D ), 60 hpf ( E ), and 84 hpf ( F ). Scale bar, 5 μm. G-I , The threshold images of VAMP2-EGFP signals in D-F for evaluation of VAMP2-EGFP puncta. J-O , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG-caCN ( J-L ) or Pomp-VG-PKA * ( M-O ) at 36 hpf ( J , M ), 60 hpf ( K , N ), and 84 hpf ( L , O ). Arrowheads point to axonal shafts. P , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-caCN (black bars), and Pomp-VG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 37, 26, and 30 at 36, 60, and 84 hpf, respectively, 21-23 embryos; Pomp-VG-caCN, n = 40, 34, and 34 at 36, 60, and 84 hpf, respectively, 24-26 embryos; Pomp-VG-PKA * , n = 25, 37, and 39 at 36, 60, and 84 hpf, respectively, 20-22 embryos. All values represent mean ± SEM. ** p
    Pbluescript Ii Sk Phagemide, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Stratagene pbluescript ii sk phagemid
    Effects of constitutively active calcineurin and constitutively active PKA on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for VAMP2-EGFP (top), for caCN and VAMP2-EGFP (middle), and for PKA * and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, <t>pBluescript</t> II SK+. B , A single axon terminal of an olfactory sensory neuron in a zebrafish embryo injected with omp promoter-driven VAMP2-EGFP and Bassoon-EYFP vectors at 50 hpf. VAMP2-ECFP (left; green) and Bassoon-EYFP (middle; red) signals were merged on the right. Arrowheads point to axonal shafts. Scale bar, 5 μm. C , Confocal fluorescent images of immunostaining with anti-GFP (left; green) and anti-syntaxin (middle; red) antibodies in the olfactory bulb from an omp promoter-driven VAMP2-ECFP transgenic fish embryo at 60 hpf. Merged image is on the right. Scale bar, 10 μm. D-F , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in Pomp-VG-injected embryos at 36 hpf ( D ), 60 hpf ( E ), and 84 hpf ( F ). Scale bar, 5 μm. G-I , The threshold images of VAMP2-EGFP signals in D-F for evaluation of VAMP2-EGFP puncta. J-O , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG-caCN ( J-L ) or Pomp-VG-PKA * ( M-O ) at 36 hpf ( J , M ), 60 hpf ( K , N ), and 84 hpf ( L , O ). Arrowheads point to axonal shafts. P , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-caCN (black bars), and Pomp-VG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 37, 26, and 30 at 36, 60, and 84 hpf, respectively, 21-23 embryos; Pomp-VG-caCN, n = 40, 34, and 34 at 36, 60, and 84 hpf, respectively, 24-26 embryos; Pomp-VG-PKA * , n = 25, 37, and 39 at 36, 60, and 84 hpf, respectively, 20-22 embryos. All values represent mean ± SEM. ** p
    Pbluescript Ii Sk Phagemid, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbluescript ii sk phagemid/product/Stratagene
    Average 85 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    DNase I footprinting analyses of protein-DNA interactions on the DS fragment. A , pKS-AHF contains a 251-bp sequence (HinfI site at 8945 nt; AluI site at 9195 nt of the EBV B95-8 genome) carrying DS on the pBluescript vector. B , a 0.44-kbp DNA of pKS-AHF

    Journal: The Journal of Biological Chemistry

    Article Title: Epstein-Barr Nuclear Antigen 1 (EBNA1)-dependent Recruitment of Origin Recognition Complex (Orc) on oriP of Epstein-Barr Virus with Purified Proteins

    doi: 10.1074/jbc.M112.368456

    Figure Lengend Snippet: DNase I footprinting analyses of protein-DNA interactions on the DS fragment. A , pKS-AHF contains a 251-bp sequence (HinfI site at 8945 nt; AluI site at 9195 nt of the EBV B95-8 genome) carrying DS on the pBluescript vector. B , a 0.44-kbp DNA of pKS-AHF

    Article Snippet: An oriP plasmid, pKS-EX, and its deletion derivatives, pKS-EXΔDS and pKS-DS, as well as EBNA1-encoding DNA (derived from the EBV strain B95-8) were kindly provided by Dr. Shirakata ( ). pKS-ARV, a DS-containing plasmid, was created by inserting an EcoRV-AluI fragment (8995–9195 nt of EBV B95-8) into a blunted SalI site of pBluescript II KS(−) vector (Stratagene). pKS-AHF, another DS-containing plasmid, was created by inserting a blunted HinfI-AluI fragment (8945–9195 nt of EBV B95-8) between SmaI and blunted SalI sites of pBluescript II KS(−). pSOP-T48 was made by replacing the bla-Lac Z′ (2126–2657 nt) portion of pBluescript II SK(−) with the SV-neo (G418R ) unit from pMAMneo (Stratagene), followed by inserting 48x tet O ( ) between SalI and XhoI sites and full-length oriP (from pKS-EX) between EcoRI and XbaI sites of its multicloning site.

    Techniques: Footprinting, Sequencing, Plasmid Preparation

    Schematic representations of PAX6 and PAX6(5a) and plasmids used as internal standards. A : Structure of PAX6, 422 amino acids and PAX6(5a), 436 amino acids. Exon 5a encodes a 14 amino acid insertion into the PAI subdomain of the paired domain disrupting its ability to bind DNA. RED subdomain and homeodomain (HD) are also shown. B : Internal standards (mimics) were created by subcloning three copies of a synthetic oligonucleotide into a unique Pst I site of the PAX6 and PAX6(5a) PCR generated fragments in pBluescript SK II as described in the Materials and Methods. The sizes of PCR products for PAX6, mimic PAX6, PAX6(5a) and mimic PAX6(5a) are 289, 431, 331, and 481 bases, respectively.

    Journal: Molecular vision

    Article Title: Quantitation of PAX6 and PAX6(5a) transcript levels in adult human lens, cornea, and monkey retina

    doi:

    Figure Lengend Snippet: Schematic representations of PAX6 and PAX6(5a) and plasmids used as internal standards. A : Structure of PAX6, 422 amino acids and PAX6(5a), 436 amino acids. Exon 5a encodes a 14 amino acid insertion into the PAI subdomain of the paired domain disrupting its ability to bind DNA. RED subdomain and homeodomain (HD) are also shown. B : Internal standards (mimics) were created by subcloning three copies of a synthetic oligonucleotide into a unique Pst I site of the PAX6 and PAX6(5a) PCR generated fragments in pBluescript SK II as described in the Materials and Methods. The sizes of PCR products for PAX6, mimic PAX6, PAX6(5a) and mimic PAX6(5a) are 289, 431, 331, and 481 bases, respectively.

    Article Snippet: Oligonucleotides used for PAX6/PAX6(5a) (AGC TAT CGA TGG CAG AAG ATT GTA GAG and CGG GAT CCG ATG ACA CGC TTG GTA TG) and TBP (CGG GAT CCT TCC ACT CAC AGA CTC TCA C and CGG AAT TCG CTC TCT TAT CCT CAT GAT TAC C) amplification, were extended with non-complementary sequences (blue) with restriction sites ClaI, BamHI , and EcoRI (red) and subcloned as a ClaI-BamHI (PAX6) and a BamHI-EcoRI (TBP) fragments into a pBluescript SK II (Stratagene, LaJolla, CA).

    Techniques: Subcloning, Polymerase Chain Reaction, Generated

    Fluorescence of PHB-producing cells grown on a LipidGreen-containing agar plate. a Fluorescence staining of PHB-producing and PHB-nonproducing cells on an agar plate. E. coli harboring pPhaCAB ( left side of the plate) had bright fluorescence under UV light (302 nm) in contrast to E. coli containing pBluescript II SK+ vector ( right side of the plate) exhibiting a much weaker fluorescence than above. b Microscopic observation of E. coli stained by LipidGreen1 on agar plates. The cell suspension in PBS buffer was placed on a glass slide to obtain optic ( left ) and fluorescent ( right ) images. The images were viewed under the fluorescence microscope (×1,000 amplification) with a green fluorescence filter.

    Journal: AMB Express

    Article Title: A lipophilic fluorescent LipidGreen1-based quantification method for high-throughput screening analysis of intracellular poly-3-hydroxybutyrate

    doi: 10.1186/s13568-015-0131-6

    Figure Lengend Snippet: Fluorescence of PHB-producing cells grown on a LipidGreen-containing agar plate. a Fluorescence staining of PHB-producing and PHB-nonproducing cells on an agar plate. E. coli harboring pPhaCAB ( left side of the plate) had bright fluorescence under UV light (302 nm) in contrast to E. coli containing pBluescript II SK+ vector ( right side of the plate) exhibiting a much weaker fluorescence than above. b Microscopic observation of E. coli stained by LipidGreen1 on agar plates. The cell suspension in PBS buffer was placed on a glass slide to obtain optic ( left ) and fluorescent ( right ) images. The images were viewed under the fluorescence microscope (×1,000 amplification) with a green fluorescence filter.

    Article Snippet: Plasmids, bacteria and chemicals The plasmid pPhaCAB consists of a pBluescript II SK+ backbone (Stratagene, USA) and the PHB biosynthetic gene cluster encoding three genes for type I PHA synthase (phaC ), ketothiolase (phaA ), and acetoacetyl-CoA reductase (phaB ) from Cupriavidus necator H16 (Yang et al. ).

    Techniques: Fluorescence, Staining, Plasmid Preparation, Microscopy, Amplification

    Constructions of recombinant WHV-HBV genomes and transient transfection of recombinant WHV-HBV genomes in hepatoma cells. (A) The schematic map of recombinant WHV-HBV genomes. pBS-WHV1.3 contained a 1.3 fold overlength WHV genome in pBluescript II SK(+) vector and was used as backbone. The respective WHV genome regions were replaced by the corresponding HBV sequences, shown as black bars. The plasmids pWHV-HBV-Sa, pWHV-HBV-SS, and pWHV-HBV-MS contained HBV sequences encoding HBsAg a-determinant only, major HBsAg and middle HBsAg, respectively. (B) HBsAg expression in the supernatant of the transfected hepatoma cells. Huh7 and HepG2 cells were transiently transfected with plasmids of pBS-WHV1.3 (W1.3), pWHV-HBV-Sa (Sa), pWHV-HBV-SS (SS), pWHV-HBV-MS (MS), pBS-HBV1.3 (H1.3), and pHBc (HBc). The culture supernatants were collected at 24, 48, 72, and 96 hours after transfection for the detection of HBsAg by ELISA. The results were read at OD 450 nm. The cut off value was set as 0.1 and indicated by the dotted line.

    Journal: PLoS ONE

    Article Title: Persistence of the Recombinant Genomes of Woodchuck Hepatitis Virus in the Mouse Model

    doi: 10.1371/journal.pone.0125658

    Figure Lengend Snippet: Constructions of recombinant WHV-HBV genomes and transient transfection of recombinant WHV-HBV genomes in hepatoma cells. (A) The schematic map of recombinant WHV-HBV genomes. pBS-WHV1.3 contained a 1.3 fold overlength WHV genome in pBluescript II SK(+) vector and was used as backbone. The respective WHV genome regions were replaced by the corresponding HBV sequences, shown as black bars. The plasmids pWHV-HBV-Sa, pWHV-HBV-SS, and pWHV-HBV-MS contained HBV sequences encoding HBsAg a-determinant only, major HBsAg and middle HBsAg, respectively. (B) HBsAg expression in the supernatant of the transfected hepatoma cells. Huh7 and HepG2 cells were transiently transfected with plasmids of pBS-WHV1.3 (W1.3), pWHV-HBV-Sa (Sa), pWHV-HBV-SS (SS), pWHV-HBV-MS (MS), pBS-HBV1.3 (H1.3), and pHBc (HBc). The culture supernatants were collected at 24, 48, 72, and 96 hours after transfection for the detection of HBsAg by ELISA. The results were read at OD 450 nm. The cut off value was set as 0.1 and indicated by the dotted line.

    Article Snippet: Construction of recombinant WHV-HBV genomes A 1.3 fold overlength WHV genome (nt 1050–2190, GenBank accession no. J04514) was cloned into pBluescript II SK(+) (Agilent Technologies, CA) at the restriction sites Kpn I and Eag І, resulting in a new vector pBS-WHV1.3 (kindly provided by Dr. Zhongji Meng).

    Techniques: Recombinant, Transfection, Plasmid Preparation, Mass Spectrometry, Expressing, Enzyme-linked Immunosorbent Assay

    Electrophoretic mobility shift assay using the PinifC3 promoter. Labeled double-stranded DNA from the −151 to −92 region of PinifC3 was mixed with 10 μg of nuclear protein from cleaving sporangia and increasing amounts of unlabeled competitor DNA representing an approximately 80- to 1,280-fold excess. The competitors are, left to right, the normal −151 to −92 region (wild type); the −151 to −92 region containing an altered 7-nt cold box (mutated CB); and the pBluescript II SK(+) multiple cloning site (nonspecific). The graphs below the gel show quantitations of bands a and b (left and right panels, respectively), using increasing excess wild-type DNA competitor (circles), cold-box-mutated competitor (squares), and nonspecific competitor (diamonds).

    Journal: Eukaryotic Cell

    Article Title: Activation of Zoosporogenesis-Specific Genes in Phytophthora infestans Involves a 7-Nucleotide Promoter Motif and Cold-Induced Membrane Rigidity

    doi: 10.1128/EC.5.4.745-752.2006

    Figure Lengend Snippet: Electrophoretic mobility shift assay using the PinifC3 promoter. Labeled double-stranded DNA from the −151 to −92 region of PinifC3 was mixed with 10 μg of nuclear protein from cleaving sporangia and increasing amounts of unlabeled competitor DNA representing an approximately 80- to 1,280-fold excess. The competitors are, left to right, the normal −151 to −92 region (wild type); the −151 to −92 region containing an altered 7-nt cold box (mutated CB); and the pBluescript II SK(+) multiple cloning site (nonspecific). The graphs below the gel show quantitations of bands a and b (left and right panels, respectively), using increasing excess wild-type DNA competitor (circles), cold-box-mutated competitor (squares), and nonspecific competitor (diamonds).

    Article Snippet: Competitors were from either the native PinifC3 promoter, the mutated version present in plasmid p141M, or pBluescript II SK+ (Invitrogen).

    Techniques: Electrophoretic Mobility Shift Assay, Labeling, Clone Assay

    SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and GFC analysis of PAP. (A) SDS-PAGE analysis. A gradient gel (8 to 16% PAGE) was used for SDS-PAGE. Lanes: M, molecular mass markers; C, whole-cell extract of E. coli harboring pBluescript SK(+)

    Journal: Journal of Bacteriology

    Article Title: Polyphosphate:AMP Phosphotransferase as a Polyphosphate-Dependent Nucleoside Monophosphate Kinase in Acinetobacter johnsonii 210A

    doi: 10.1128/JB.187.5.1859-1865.2005

    Figure Lengend Snippet: SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and GFC analysis of PAP. (A) SDS-PAGE analysis. A gradient gel (8 to 16% PAGE) was used for SDS-PAGE. Lanes: M, molecular mass markers; C, whole-cell extract of E. coli harboring pBluescript SK(+)

    Article Snippet: The purified DNA fragments were ligated into the BamHI site of the pBluescript II SK(+) cloning vector (Toyobo, Osaka, Japan).

    Techniques: Polyacrylamide Gel Electrophoresis, SDS Page

    Effects of constitutively active calcineurin and constitutively active PKA on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for VAMP2-EGFP (top), for caCN and VAMP2-EGFP (middle), and for PKA * and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, pBluescript II SK+. B , A single axon terminal of an olfactory sensory neuron in a zebrafish embryo injected with omp promoter-driven VAMP2-EGFP and Bassoon-EYFP vectors at 50 hpf. VAMP2-ECFP (left; green) and Bassoon-EYFP (middle; red) signals were merged on the right. Arrowheads point to axonal shafts. Scale bar, 5 μm. C , Confocal fluorescent images of immunostaining with anti-GFP (left; green) and anti-syntaxin (middle; red) antibodies in the olfactory bulb from an omp promoter-driven VAMP2-ECFP transgenic fish embryo at 60 hpf. Merged image is on the right. Scale bar, 10 μm. D-F , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in Pomp-VG-injected embryos at 36 hpf ( D ), 60 hpf ( E ), and 84 hpf ( F ). Scale bar, 5 μm. G-I , The threshold images of VAMP2-EGFP signals in D-F for evaluation of VAMP2-EGFP puncta. J-O , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG-caCN ( J-L ) or Pomp-VG-PKA * ( M-O ) at 36 hpf ( J , M ), 60 hpf ( K , N ), and 84 hpf ( L , O ). Arrowheads point to axonal shafts. P , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-caCN (black bars), and Pomp-VG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 37, 26, and 30 at 36, 60, and 84 hpf, respectively, 21-23 embryos; Pomp-VG-caCN, n = 40, 34, and 34 at 36, 60, and 84 hpf, respectively, 24-26 embryos; Pomp-VG-PKA * , n = 25, 37, and 39 at 36, 60, and 84 hpf, respectively, 20-22 embryos. All values represent mean ± SEM. ** p

    Journal: The Journal of Neuroscience

    Article Title: Distinct Roles of Calcineurin-Nuclear Factor of Activated T-Cells and Protein Kinase A-cAMP Response Element-Binding Protein Signaling in Presynaptic Differentiation

    doi: 10.1523/JNEUROSCI.3738-04.2005

    Figure Lengend Snippet: Effects of constitutively active calcineurin and constitutively active PKA on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for VAMP2-EGFP (top), for caCN and VAMP2-EGFP (middle), and for PKA * and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, pBluescript II SK+. B , A single axon terminal of an olfactory sensory neuron in a zebrafish embryo injected with omp promoter-driven VAMP2-EGFP and Bassoon-EYFP vectors at 50 hpf. VAMP2-ECFP (left; green) and Bassoon-EYFP (middle; red) signals were merged on the right. Arrowheads point to axonal shafts. Scale bar, 5 μm. C , Confocal fluorescent images of immunostaining with anti-GFP (left; green) and anti-syntaxin (middle; red) antibodies in the olfactory bulb from an omp promoter-driven VAMP2-ECFP transgenic fish embryo at 60 hpf. Merged image is on the right. Scale bar, 10 μm. D-F , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in Pomp-VG-injected embryos at 36 hpf ( D ), 60 hpf ( E ), and 84 hpf ( F ). Scale bar, 5 μm. G-I , The threshold images of VAMP2-EGFP signals in D-F for evaluation of VAMP2-EGFP puncta. J-O , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG-caCN ( J-L ) or Pomp-VG-PKA * ( M-O ) at 36 hpf ( J , M ), 60 hpf ( K , N ), and 84 hpf ( L , O ). Arrowheads point to axonal shafts. P , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-caCN (black bars), and Pomp-VG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 37, 26, and 30 at 36, 60, and 84 hpf, respectively, 21-23 embryos; Pomp-VG-caCN, n = 40, 34, and 34 at 36, 60, and 84 hpf, respectively, 24-26 embryos; Pomp-VG-PKA * , n = 25, 37, and 39 at 36, 60, and 84 hpf, respectively, 20-22 embryos. All values represent mean ± SEM. ** p

    Article Snippet: The 0.4 kb Pvu II fragment from pBluescript II SK+ carrying multicloning site was inserted between the Pvu II and Sna BI sites of pGBT9 vector (Clontech) to yield pGBT-MCS.

    Techniques: Expressing, Sequencing, Injection, Immunostaining, Transgenic Assay, Fluorescence In Situ Hybridization

    Effects of dominant-negative PKA on the VAMP2-EGFP puncta formation in axon terminals of olfactory sensory neurons. A , Omp promoter-driven double-cassette expression vector for VAMP2-EGFP and dominant-negative PKA. Black boxes, The omp promoter; cross-hatched box, the 3′ downstream sequence of the omp gene; hatched box, SV40 polyadenylation signal sequence; line, pBluescript II SK+. B-G , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG ( B-D ) or Pomp-VG-dnPKA ( E-G ) at 36 hpf ( B , E ), 60 hpf ( C , F ), and 84 hpf ( D , G ). Arrowheads point to axonal shafts. Scale bar, 5 μm. H , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars) and Pomp-VG-dnPKA (filled bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 31, 33, and 34 at 36, 60, and 84 hpf, respectively, 25-31 embryos; Pomp-VG-dnPKA, n = 35, 35, and 35 at 36, 60, and 84 hpf, respectively, 25-30 embryos. All values represent mean ± SEM. * p

    Journal: The Journal of Neuroscience

    Article Title: Distinct Roles of Calcineurin-Nuclear Factor of Activated T-Cells and Protein Kinase A-cAMP Response Element-Binding Protein Signaling in Presynaptic Differentiation

    doi: 10.1523/JNEUROSCI.3738-04.2005

    Figure Lengend Snippet: Effects of dominant-negative PKA on the VAMP2-EGFP puncta formation in axon terminals of olfactory sensory neurons. A , Omp promoter-driven double-cassette expression vector for VAMP2-EGFP and dominant-negative PKA. Black boxes, The omp promoter; cross-hatched box, the 3′ downstream sequence of the omp gene; hatched box, SV40 polyadenylation signal sequence; line, pBluescript II SK+. B-G , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG ( B-D ) or Pomp-VG-dnPKA ( E-G ) at 36 hpf ( B , E ), 60 hpf ( C , F ), and 84 hpf ( D , G ). Arrowheads point to axonal shafts. Scale bar, 5 μm. H , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars) and Pomp-VG-dnPKA (filled bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 31, 33, and 34 at 36, 60, and 84 hpf, respectively, 25-31 embryos; Pomp-VG-dnPKA, n = 35, 35, and 35 at 36, 60, and 84 hpf, respectively, 25-30 embryos. All values represent mean ± SEM. * p

    Article Snippet: The 0.4 kb Pvu II fragment from pBluescript II SK+ carrying multicloning site was inserted between the Pvu II and Sna BI sites of pGBT9 vector (Clontech) to yield pGBT-MCS.

    Techniques: Dominant Negative Mutation, Expressing, Plasmid Preparation, Sequencing, Injection

    Effects of constitutively active calcineurin and constitutively active PKA on the axon terminal morphology of olfactory sensory neurons in living zebrafish embryos. A , Omp promoter-driven expression vectors for GAP43-EGFP (top), for caCN and GAP43-EGFP (middle), and for PKA * and GAP43-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, pBluescript II SK+. B-J , Representative images of the axon terminal morphology of olfactory sensory neurons in zebrafish embryos injected with Pomp-GG ( B-D ), Pomp-GG-caCN ( E-G ), or Pomp-GG-PKA * ( H-J ) vectors at 36 hpf ( B , E , H ), 60 hpf ( C , F , I ), and 84 hpf ( D , G , J ). Scale bar, 5 μm. K-M , The area ( K ), perimeter ( L ), and complexity ( M ) values of axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-GG (open bars), Pomp-GG-caCN (black bars), and Pomp-GG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-GG, n = 25, 29, and 28 at 36, 60, and 84 hpf, respectively, 20 embryos; Pomp-GG-caCN, n = 26, 21, and 25 at 36, 60, and 84 hpf, respectively, 17-20 embryos; Pomp-GG-PKA * , n = 19, 21, and 29 at 36, 60, and 84 hpf, respectively, 14-18 embryos. All values represent mean ± SEM. * p

    Journal: The Journal of Neuroscience

    Article Title: Distinct Roles of Calcineurin-Nuclear Factor of Activated T-Cells and Protein Kinase A-cAMP Response Element-Binding Protein Signaling in Presynaptic Differentiation

    doi: 10.1523/JNEUROSCI.3738-04.2005

    Figure Lengend Snippet: Effects of constitutively active calcineurin and constitutively active PKA on the axon terminal morphology of olfactory sensory neurons in living zebrafish embryos. A , Omp promoter-driven expression vectors for GAP43-EGFP (top), for caCN and GAP43-EGFP (middle), and for PKA * and GAP43-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, pBluescript II SK+. B-J , Representative images of the axon terminal morphology of olfactory sensory neurons in zebrafish embryos injected with Pomp-GG ( B-D ), Pomp-GG-caCN ( E-G ), or Pomp-GG-PKA * ( H-J ) vectors at 36 hpf ( B , E , H ), 60 hpf ( C , F , I ), and 84 hpf ( D , G , J ). Scale bar, 5 μm. K-M , The area ( K ), perimeter ( L ), and complexity ( M ) values of axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-GG (open bars), Pomp-GG-caCN (black bars), and Pomp-GG-PKA * (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-GG, n = 25, 29, and 28 at 36, 60, and 84 hpf, respectively, 20 embryos; Pomp-GG-caCN, n = 26, 21, and 25 at 36, 60, and 84 hpf, respectively, 17-20 embryos; Pomp-GG-PKA * , n = 19, 21, and 29 at 36, 60, and 84 hpf, respectively, 14-18 embryos. All values represent mean ± SEM. * p

    Article Snippet: The 0.4 kb Pvu II fragment from pBluescript II SK+ carrying multicloning site was inserted between the Pvu II and Sna BI sites of pGBT9 vector (Clontech) to yield pGBT-MCS.

    Techniques: Expressing, Sequencing, Injection

    Effects of suppression of NFAT on the axon terminal morphology of olfactory sensory neurons. A , Omp promoter-driven expression vectors for GST-CP and GAP43-EGFP (top) and for GST-VIVIT and GAP43-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, pBluescript II SK+. B-E , Representative GAP43-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-GG-GST-CP ( B , C ) or Pomp-GG-GST-VIVIT ( D , E ) at 60 hpf ( B , D ) and 84 hpf ( C , E ). Scale bar, 5 μm. F-H , The area ( F ), perimeter ( G ), and complexity ( H ) values of axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-GG-GST-CP (open bars) and Pomp-GG-GST-VIVIT (black bars) expression vectors at 36, 60, and 84 hpf. Pomp-GG-GST-CP, n = 41, 36, and 39 at 36, 60, and 84 hpf, respectively, 23-25 embryos; Pomp-GG-GST-VIVIT, n = 37, 41, and 39 at 36, 60, and 84 hpf, respectively, 24-30 embryos. All values represent mean ± SEM. *** p

    Journal: The Journal of Neuroscience

    Article Title: Distinct Roles of Calcineurin-Nuclear Factor of Activated T-Cells and Protein Kinase A-cAMP Response Element-Binding Protein Signaling in Presynaptic Differentiation

    doi: 10.1523/JNEUROSCI.3738-04.2005

    Figure Lengend Snippet: Effects of suppression of NFAT on the axon terminal morphology of olfactory sensory neurons. A , Omp promoter-driven expression vectors for GST-CP and GAP43-EGFP (top) and for GST-VIVIT and GAP43-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, pBluescript II SK+. B-E , Representative GAP43-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-GG-GST-CP ( B , C ) or Pomp-GG-GST-VIVIT ( D , E ) at 60 hpf ( B , D ) and 84 hpf ( C , E ). Scale bar, 5 μm. F-H , The area ( F ), perimeter ( G ), and complexity ( H ) values of axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-GG-GST-CP (open bars) and Pomp-GG-GST-VIVIT (black bars) expression vectors at 36, 60, and 84 hpf. Pomp-GG-GST-CP, n = 41, 36, and 39 at 36, 60, and 84 hpf, respectively, 23-25 embryos; Pomp-GG-GST-VIVIT, n = 37, 41, and 39 at 36, 60, and 84 hpf, respectively, 24-30 embryos. All values represent mean ± SEM. *** p

    Article Snippet: The 0.4 kb Pvu II fragment from pBluescript II SK+ carrying multicloning site was inserted between the Pvu II and Sna BI sites of pGBT9 vector (Clontech) to yield pGBT-MCS.

    Techniques: Expressing, Sequencing, Injection

    Effects of dominant-negative CREB and constitutively active CREB on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for A-CREB and VAMP2-EGFP (top) and for VP16-CREB and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, pBluescript II SK+. B-J , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in embryos injected with Pomp-VG ( B-D ), Pomp-VG-ACREB ( E-G ), or Pomp-VG-VP16CREB ( H-J ) at 36 hpf ( B , E , H ), 60 hpf ( C , F , I ), and 84 hpf ( D , G , J ). Arrowheads point to axonal shafts. Scale bar, 5 μm. K , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-ACREB (black bars), and Pomp-VG-VP16CREB (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 32, 35, and 37 at 36, 60, and 84 hpf, respectively, 22-26 embryos; Pomp-VG-ACREB, n = 35, 41, and 36 at 36, 60, and 84 hpf, respectively, 18-19 embryos; Pomp-VG-VP16CREB, n = 33,33, and 34 at 36,60, and 84 hpf, respectively,22-27 embryos. All values represent mean ± SEM. ** p

    Journal: The Journal of Neuroscience

    Article Title: Distinct Roles of Calcineurin-Nuclear Factor of Activated T-Cells and Protein Kinase A-cAMP Response Element-Binding Protein Signaling in Presynaptic Differentiation

    doi: 10.1523/JNEUROSCI.3738-04.2005

    Figure Lengend Snippet: Effects of dominant-negative CREB and constitutively active CREB on the development of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons. A , Omp promoter-driven expression vectors for A-CREB and VAMP2-EGFP (top) and for VP16-CREB and VAMP2-EGFP (bottom) in olfactory sensory neurons. Black boxes, The omp promoter; cross-hatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, pBluescript II SK+. B-J , Representative VAMP2-EGFP signals in axon terminals of olfactory sensory neurons in embryos injected with Pomp-VG ( B-D ), Pomp-VG-ACREB ( E-G ), or Pomp-VG-VP16CREB ( H-J ) at 36 hpf ( B , E , H ), 60 hpf ( C , F , I ), and 84 hpf ( D , G , J ). Arrowheads point to axonal shafts. Scale bar, 5 μm. K , The area of VAMP2-EGFP puncta in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-VG (open bars), Pomp-VG-ACREB (black bars), and Pomp-VG-VP16CREB (gray bars) expression vectors at 36, 60, and 84 hpf. Pomp-VG, n = 32, 35, and 37 at 36, 60, and 84 hpf, respectively, 22-26 embryos; Pomp-VG-ACREB, n = 35, 41, and 36 at 36, 60, and 84 hpf, respectively, 18-19 embryos; Pomp-VG-VP16CREB, n = 33,33, and 34 at 36,60, and 84 hpf, respectively,22-27 embryos. All values represent mean ± SEM. ** p

    Article Snippet: The 0.4 kb Pvu II fragment from pBluescript II SK+ carrying multicloning site was inserted between the Pvu II and Sna BI sites of pGBT9 vector (Clontech) to yield pGBT-MCS.

    Techniques: Dominant Negative Mutation, Expressing, Sequencing, Injection