pae Search Results


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  • c pae  (ATCC)
    99
    ATCC c pae
    Internalization of ES, ES-BAX, ES-BAK, and ES-BAX-ES by endothelial cells. ( a ) <t>C-PAE</t> or <t>NIH</t> 3T3 cells were incubated at 37°C for 2 h with 10 μ g/ml of the biotynilated proteins: ES, ES-BAX, ES-BAK, or ES-BAX-ES. The cells were then fixed, permeabilized, and incubated with Alexa 488 conjugated with streptavidin. The nucleuses were stained with DAPI. The cells were analyzed under a fluorescence microscope. Scale bar: 10 μ m. ( b ) Western blot of endothelial C-PAE cells incubated with 10 μ g/ml of the indicated proteins at 37°C and detected by anti-ES antibody. Control (+): ES; control (−): lysate of cells incubated with ES that were immediately processed for western blot ( t =0 min)
    C Pae, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore paeoniflorin pae
    Internalization of ES, ES-BAX, ES-BAK, and ES-BAX-ES by endothelial cells. ( a ) <t>C-PAE</t> or <t>NIH</t> 3T3 cells were incubated at 37°C for 2 h with 10 μ g/ml of the biotynilated proteins: ES, ES-BAX, ES-BAK, or ES-BAX-ES. The cells were then fixed, permeabilized, and incubated with Alexa 488 conjugated with streptavidin. The nucleuses were stained with DAPI. The cells were analyzed under a fluorescence microscope. Scale bar: 10 μ m. ( b ) Western blot of endothelial C-PAE cells incubated with 10 μ g/ml of the indicated proteins at 37°C and detected by anti-ES antibody. Control (+): ES; control (−): lysate of cells incubated with ES that were immediately processed for western blot ( t =0 min)
    Paeoniflorin Pae, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Illumina Inc e pae illumina
    Plasmid profiles of <t>Pae</t> . Agarose gel electrophoresis was carried out as described by [33] to compare the number and size of native plasmids present within the genomes of each of the four Pae strains; P. syringae pv. phaseolicola strain <t>1448A</t> was included for comparison. M represents marker plasmids from Escherichia coli strain 39R861 [60] . Note that E- Pae strains 2250 and P6623 have two similarly sized plasmids of ca . 70 kb.
    E Pae Illumina, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    CMA Microdialysis polyarylethersulfone paes
    Plasmid profiles of <t>Pae</t> . Agarose gel electrophoresis was carried out as described by [33] to compare the number and size of native plasmids present within the genomes of each of the four Pae strains; P. syringae pv. phaseolicola strain <t>1448A</t> was included for comparison. M represents marker plasmids from Escherichia coli strain 39R861 [60] . Note that E- Pae strains 2250 and P6623 have two similarly sized plasmids of ca . 70 kb.
    Polyarylethersulfone Paes, supplied by CMA Microdialysis, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pae  (DeWALT)
    91
    DeWALT pae
    Plasmid profiles of <t>Pae</t> . Agarose gel electrophoresis was carried out as described by [33] to compare the number and size of native plasmids present within the genomes of each of the four Pae strains; P. syringae pv. phaseolicola strain <t>1448A</t> was included for comparison. M represents marker plasmids from Escherichia coli strain 39R861 [60] . Note that E- Pae strains 2250 and P6623 have two similarly sized plasmids of ca . 70 kb.
    Pae, supplied by DeWALT, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher pae cells
    PKPD modelled exposure–response relationships for BP elevation at steady state corrected for <t>VEGFR‐2</t> potency: multiple PK parameters and VEGFR‐2 assays. Each graph shows effects of four different VEGFR‐2 inhibitors at steady state derived from rat telemetry or clinical studies. The X‐axis by row from top to bottom is C av,u , C max,u and AUC 0–24,u in μM; by column going from left to right is the normalization applied to the PK metric using the IC 50 of the VEGFR‐2 assays: HUVEC, SPR and <t>PAE.</t> Data were plotted as the dose response of the calculated PK metric assay value normalized for VEGFR‐2 IC 50 versus BP elevation (in mmHg). Curves are coloured according to the key shown, which describes the VEGFR‐2 inhibitor, the report and the species where the model was developed. The rat telemetry study performed to support this work (as described in the text) is denoted by ‘this study’.
    Pae Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Anhui Medical University pae
    CP-25 treatment attenuates clinical signs in rats with AA. ( A ) Chemical structures of CP-25 and <t>Pae.</t> CP-25 (C 29 H 32 O 13 S, MW: 620); Pae (C 23 H 28 O 11 , MW: 480.45). (B) Photographs of representative paws from each AA group. (C,D) Arthritic scores of immunized Lewis rats treated either with drugs or vehicle beginning at the onset of disease (day 17) and continuing for 13 days. CP-25, <t>TGP</t> and Pae were administered intragastrically each day, and MTX was given intragastrically once every 3 days. Swelling of the non-injected hind paw and the polyarthritis index were assessed at 3-day intervals. # P
    Pae, supplied by Anhui Medical University, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson pae
    CP-25 treatment attenuates clinical signs in rats with AA. ( A ) Chemical structures of CP-25 and <t>Pae.</t> CP-25 (C 29 H 32 O 13 S, MW: 620); Pae (C 23 H 28 O 11 , MW: 480.45). (B) Photographs of representative paws from each AA group. (C,D) Arthritic scores of immunized Lewis rats treated either with drugs or vehicle beginning at the onset of disease (day 17) and continuing for 13 days. CP-25, <t>TGP</t> and Pae were administered intragastrically each day, and MTX was given intragastrically once every 3 days. Swelling of the non-injected hind paw and the polyarthritis index were assessed at 3-day intervals. # P
    Pae, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Philips Healthcare pae
    CP-25 treatment attenuates clinical signs in rats with AA. ( A ) Chemical structures of CP-25 and <t>Pae.</t> CP-25 (C 29 H 32 O 13 S, MW: 620); Pae (C 23 H 28 O 11 , MW: 480.45). (B) Photographs of representative paws from each AA group. (C,D) Arthritic scores of immunized Lewis rats treated either with drugs or vehicle beginning at the onset of disease (day 17) and continuing for 13 days. CP-25, <t>TGP</t> and Pae were administered intragastrically each day, and MTX was given intragastrically once every 3 days. Swelling of the non-injected hind paw and the polyarthritis index were assessed at 3-day intervals. # P
    Pae, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Respiratory Motion pae
    CP-25 treatment attenuates clinical signs in rats with AA. ( A ) Chemical structures of CP-25 and <t>Pae.</t> CP-25 (C 29 H 32 O 13 S, MW: 620); Pae (C 23 H 28 O 11 , MW: 480.45). (B) Photographs of representative paws from each AA group. (C,D) Arthritic scores of immunized Lewis rats treated either with drugs or vehicle beginning at the onset of disease (day 17) and continuing for 13 days. CP-25, <t>TGP</t> and Pae were administered intragastrically each day, and MTX was given intragastrically once every 3 days. Swelling of the non-injected hind paw and the polyarthritis index were assessed at 3-day intervals. # P
    Pae, supplied by Respiratory Motion, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Brookhaven Instruments pae nps
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Pae Nps, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 97/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Rohner pae we
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Pae We, supplied by Rohner, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Shimadzu Corporation pae nps
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Pae Nps, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    3-D Matrix pae surface
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Pae Surface, supplied by 3-D Matrix, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore paes
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Paes, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher p aeruginosa pao1 pae g1a
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    P Aeruginosa Pao1 Pae G1a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Johnson & Johnson pae c u
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Pae C U, supplied by Johnson & Johnson, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore c pae cells
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    C Pae Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    CMA Microdialysis mwco polyarylethersulphone paes membrane
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Mwco Polyarylethersulphone Paes Membrane, supplied by CMA Microdialysis, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Illumina Inc e pae illumina paired
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    E Pae Illumina Paired, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    InvivoGen pae kdr cells
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Pae Kdr Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    CMA Microdialysis vivo microdialysis paes
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Vivo Microdialysis Paes, supplied by CMA Microdialysis, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Waters Corporation poa pae content
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Poa Pae Content, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Pfizer Inc pae pf29 vector
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Pae Pf29 Vector, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    The Jackson Laboratory pae c57 bl6j mice
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Pae C57 Bl6j Mice, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Rudbeck Laboratory porcine endothelial cell pae
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    Porcine Endothelial Cell Pae, supplied by Rudbeck Laboratory, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore 3 cm pae 300 column
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    3 Cm Pae 300 Column, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc e pae strain 2250
    Characterization of <t>Pae-NPs.</t> Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.
    E Pae Strain 2250, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore gcn4 pae peptide
    Crystal structure of the <t>GCN4-pAe</t> tetramer
    Gcn4 Pae Peptide, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    R&D Systems human vegfr2 pae vegfr2
    Crystal structure of the <t>GCN4-pAe</t> tetramer
    Human Vegfr2 Pae Vegfr2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Internalization of ES, ES-BAX, ES-BAK, and ES-BAX-ES by endothelial cells. ( a ) C-PAE or NIH 3T3 cells were incubated at 37°C for 2 h with 10 μ g/ml of the biotynilated proteins: ES, ES-BAX, ES-BAK, or ES-BAX-ES. The cells were then fixed, permeabilized, and incubated with Alexa 488 conjugated with streptavidin. The nucleuses were stained with DAPI. The cells were analyzed under a fluorescence microscope. Scale bar: 10 μ m. ( b ) Western blot of endothelial C-PAE cells incubated with 10 μ g/ml of the indicated proteins at 37°C and detected by anti-ES antibody. Control (+): ES; control (−): lysate of cells incubated with ES that were immediately processed for western blot ( t =0 min)

    Journal: Cell Death & Disease

    Article Title: Improving the therapeutic potential of endostatin by fusing it with the BAX BH3 death domain

    doi: 10.1038/cddis.2014.309

    Figure Lengend Snippet: Internalization of ES, ES-BAX, ES-BAK, and ES-BAX-ES by endothelial cells. ( a ) C-PAE or NIH 3T3 cells were incubated at 37°C for 2 h with 10 μ g/ml of the biotynilated proteins: ES, ES-BAX, ES-BAK, or ES-BAX-ES. The cells were then fixed, permeabilized, and incubated with Alexa 488 conjugated with streptavidin. The nucleuses were stained with DAPI. The cells were analyzed under a fluorescence microscope. Scale bar: 10 μ m. ( b ) Western blot of endothelial C-PAE cells incubated with 10 μ g/ml of the indicated proteins at 37°C and detected by anti-ES antibody. Control (+): ES; control (−): lysate of cells incubated with ES that were immediately processed for western blot ( t =0 min)

    Article Snippet: C-PAE or NIH 3T3 cells (ATCC, CCL-209 and CRL-1658, respectively; 5 × 104 ) were cultured in glass coverslips in EMEM medium (Life Technologies Corporation, Grand Island, NY, USA) supplemented with 2% FBS for 24 h. After 24 h of serum deprivation, the cells were incubated for 12 h in the same medium supplemented with 5 ng/ml bFGF.

    Techniques: Incubation, Staining, Fluorescence, Microscopy, Western Blot

    Plasmid profiles of Pae . Agarose gel electrophoresis was carried out as described by [33] to compare the number and size of native plasmids present within the genomes of each of the four Pae strains; P. syringae pv. phaseolicola strain 1448A was included for comparison. M represents marker plasmids from Escherichia coli strain 39R861 [60] . Note that E- Pae strains 2250 and P6623 have two similarly sized plasmids of ca . 70 kb.

    Journal: PLoS ONE

    Article Title: Comparative Genome Analysis Provides Insights into the Evolution and Adaptation of Pseudomonas syringae pv. aesculi on Aesculus hippocastanum

    doi: 10.1371/journal.pone.0010224

    Figure Lengend Snippet: Plasmid profiles of Pae . Agarose gel electrophoresis was carried out as described by [33] to compare the number and size of native plasmids present within the genomes of each of the four Pae strains; P. syringae pv. phaseolicola strain 1448A was included for comparison. M represents marker plasmids from Escherichia coli strain 39R861 [60] . Note that E- Pae strains 2250 and P6623 have two similarly sized plasmids of ca . 70 kb.

    Article Snippet: If this proposition is true, then we can identify those Pph 1448A genes that are not conserved in Pae on the basis of their lack of coverage by Illumina reads.

    Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Marker

    An E- Pae -encoded pathway for the utilization of sucrose. Shown is a cluster of genes in E-P ae which is implicated in the uptake and utilization of sucrose but which is not found in I- Pae . Details of the predicted genes based on blastp searches are shown in Table 5 . This gene cluster is homologous to a region of Pph 448A (96–98% nucleotide sequence identity). In E- Pae the six principal genes (A–F) are on a 7.4 kb section of a 32.1 kb contig (GenBank: ACXT01000147.1) whereas the transcriptional regulator (G) is found at the beginning of a 12 kb contig (GenBank: ACXT01000532.1); (the first 67 codons are missing from the start of the contig). Genes in E- Pae are indicated by the blue arrows; genes in Pph 1448A are represented by the green arrows.

    Journal: PLoS ONE

    Article Title: Comparative Genome Analysis Provides Insights into the Evolution and Adaptation of Pseudomonas syringae pv. aesculi on Aesculus hippocastanum

    doi: 10.1371/journal.pone.0010224

    Figure Lengend Snippet: An E- Pae -encoded pathway for the utilization of sucrose. Shown is a cluster of genes in E-P ae which is implicated in the uptake and utilization of sucrose but which is not found in I- Pae . Details of the predicted genes based on blastp searches are shown in Table 5 . This gene cluster is homologous to a region of Pph 448A (96–98% nucleotide sequence identity). In E- Pae the six principal genes (A–F) are on a 7.4 kb section of a 32.1 kb contig (GenBank: ACXT01000147.1) whereas the transcriptional regulator (G) is found at the beginning of a 12 kb contig (GenBank: ACXT01000532.1); (the first 67 codons are missing from the start of the contig). Genes in E- Pae are indicated by the blue arrows; genes in Pph 1448A are represented by the green arrows.

    Article Snippet: If this proposition is true, then we can identify those Pph 1448A genes that are not conserved in Pae on the basis of their lack of coverage by Illumina reads.

    Techniques: Sequencing

    PKPD modelled exposure–response relationships for BP elevation at steady state corrected for VEGFR‐2 potency: multiple PK parameters and VEGFR‐2 assays. Each graph shows effects of four different VEGFR‐2 inhibitors at steady state derived from rat telemetry or clinical studies. The X‐axis by row from top to bottom is C av,u , C max,u and AUC 0–24,u in μM; by column going from left to right is the normalization applied to the PK metric using the IC 50 of the VEGFR‐2 assays: HUVEC, SPR and PAE. Data were plotted as the dose response of the calculated PK metric assay value normalized for VEGFR‐2 IC 50 versus BP elevation (in mmHg). Curves are coloured according to the key shown, which describes the VEGFR‐2 inhibitor, the report and the species where the model was developed. The rat telemetry study performed to support this work (as described in the text) is denoted by ‘this study’.

    Journal: British Journal of Pharmacology

    Article Title: Quantifying the relationship between inhibition of VEGF receptor 2, drug‐induced blood pressure elevation and hypertension) Quantifying the relationship between inhibition of VEGF receptor 2, drug‐induced blood pressure elevation and hypertension

    doi: 10.1111/bph.14103

    Figure Lengend Snippet: PKPD modelled exposure–response relationships for BP elevation at steady state corrected for VEGFR‐2 potency: multiple PK parameters and VEGFR‐2 assays. Each graph shows effects of four different VEGFR‐2 inhibitors at steady state derived from rat telemetry or clinical studies. The X‐axis by row from top to bottom is C av,u , C max,u and AUC 0–24,u in μM; by column going from left to right is the normalization applied to the PK metric using the IC 50 of the VEGFR‐2 assays: HUVEC, SPR and PAE. Data were plotted as the dose response of the calculated PK metric assay value normalized for VEGFR‐2 IC 50 versus BP elevation (in mmHg). Curves are coloured according to the key shown, which describes the VEGFR‐2 inhibitor, the report and the species where the model was developed. The rat telemetry study performed to support this work (as described in the text) is denoted by ‘this study’.

    Article Snippet: The purpose of the assay was to determine the potency of test compounds to inhibit VEGFR‐2 phosphorylation in PAE cells (obtained from Invitrogen, UK) overexpressing VEGFR‐2.

    Techniques: Derivative Assay, SPR Assay

    CP-25 treatment attenuates clinical signs in rats with AA. ( A ) Chemical structures of CP-25 and Pae. CP-25 (C 29 H 32 O 13 S, MW: 620); Pae (C 23 H 28 O 11 , MW: 480.45). (B) Photographs of representative paws from each AA group. (C,D) Arthritic scores of immunized Lewis rats treated either with drugs or vehicle beginning at the onset of disease (day 17) and continuing for 13 days. CP-25, TGP and Pae were administered intragastrically each day, and MTX was given intragastrically once every 3 days. Swelling of the non-injected hind paw and the polyarthritis index were assessed at 3-day intervals. # P

    Journal: Scientific Reports

    Article Title: CP-25, a novel compound, protects against autoimmune arthritis by modulating immune mediators of inflammation and bone damage

    doi: 10.1038/srep26239

    Figure Lengend Snippet: CP-25 treatment attenuates clinical signs in rats with AA. ( A ) Chemical structures of CP-25 and Pae. CP-25 (C 29 H 32 O 13 S, MW: 620); Pae (C 23 H 28 O 11 , MW: 480.45). (B) Photographs of representative paws from each AA group. (C,D) Arthritic scores of immunized Lewis rats treated either with drugs or vehicle beginning at the onset of disease (day 17) and continuing for 13 days. CP-25, TGP and Pae were administered intragastrically each day, and MTX was given intragastrically once every 3 days. Swelling of the non-injected hind paw and the polyarthritis index were assessed at 3-day intervals. # P

    Article Snippet: Reagents TGP ( > 40% purity) and Pae ( > 90% purity) were provided by the Chemistry Laboratory of the Institute of Clinical Pharmacology of Anhui Medical University (Hefei, Anhui Province, China).

    Techniques: Injection

    Characterization of Pae-NPs. Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Characterization of Pae-NPs. Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.

    Article Snippet: The solution of Pae-NPs was kept at room temperature.

    Techniques:

    Effects of free Pae or Pae-NPs on the expression of Akt pathway proteins in A549 cells. Notes: ( A ) Blots of p-Akt and Akt from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Effects of free Pae or Pae-NPs on the expression of Akt pathway proteins in A549 cells. Notes: ( A ) Blots of p-Akt and Akt from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Article Snippet: The solution of Pae-NPs was kept at room temperature.

    Techniques: Expressing, Standard Deviation

    Effect of free Pae or Pae-NPs on the expression of apoptotic proteins in A549 cells. Notes: ( A ) Blots of Bcl-XL, Bax, and Bcl-2 from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Effect of free Pae or Pae-NPs on the expression of apoptotic proteins in A549 cells. Notes: ( A ) Blots of Bcl-XL, Bax, and Bcl-2 from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Article Snippet: The solution of Pae-NPs was kept at room temperature.

    Techniques: Expressing, Standard Deviation

    Cellular uptake of coumarin-6-loaded fluorescent Pae-NPs by cancer cells. Notes: ( A , B ) Representative microscopic images of A549 cells incubated with coumarin-6-loaded Pae-NPs for 2 hours; ( A ) bright fields with higher magnification; ( B ) fluorescent fields with higher magnification. ( C ) Cellular uptake efficiency of Pae-NPs by A549 and NCI-H1975 cells. Data are presented as mean ± SD (n=3). ** p

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Cellular uptake of coumarin-6-loaded fluorescent Pae-NPs by cancer cells. Notes: ( A , B ) Representative microscopic images of A549 cells incubated with coumarin-6-loaded Pae-NPs for 2 hours; ( A ) bright fields with higher magnification; ( B ) fluorescent fields with higher magnification. ( C ) Cellular uptake efficiency of Pae-NPs by A549 and NCI-H1975 cells. Data are presented as mean ± SD (n=3). ** p

    Article Snippet: The solution of Pae-NPs was kept at room temperature.

    Techniques: Incubation

    In vivo antitumor efficacy of Pae-NPs. Notes: The mice were intravenously administered with PBS, E-NPs, free Pae, or Pae-NPs every 2 days for three consecutive injections. ( A ) Growth inhibition study in the A549 xenograft model. ( B ) Body weight changes for the tumor bearing mice after various formulations were given to mice on the indicated days. Data are represented as mean ± SD (n=6). * p

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: In vivo antitumor efficacy of Pae-NPs. Notes: The mice were intravenously administered with PBS, E-NPs, free Pae, or Pae-NPs every 2 days for three consecutive injections. ( A ) Growth inhibition study in the A549 xenograft model. ( B ) Body weight changes for the tumor bearing mice after various formulations were given to mice on the indicated days. Data are represented as mean ± SD (n=6). * p

    Article Snippet: The solution of Pae-NPs was kept at room temperature.

    Techniques: In Vivo, Mouse Assay, Inhibition

    Effect of free Pae or Pae-NPs on the expression of caspase-3 and cleaved caspase-3 proteins in A549 cells. Notes: ( A ) Blots of caspase-3 and pro-caspase-3 from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Effect of free Pae or Pae-NPs on the expression of caspase-3 and cleaved caspase-3 proteins in A549 cells. Notes: ( A ) Blots of caspase-3 and pro-caspase-3 from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Article Snippet: The solution of Pae-NPs was kept at room temperature.

    Techniques: Expressing, Standard Deviation

    Detection of intracellular ROS. Notes: ( A ) DHE fluorescence images of A549 cells treated with different agents (control; E-NPs; Pae [50 μg/mL]; Pae-NPs [at an equivalent dose of 50 μg/mL]). ( B ) Intracellular DHE fluorescence intensity. Data are represented as mean ± SD. * p

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Detection of intracellular ROS. Notes: ( A ) DHE fluorescence images of A549 cells treated with different agents (control; E-NPs; Pae [50 μg/mL]; Pae-NPs [at an equivalent dose of 50 μg/mL]). ( B ) Intracellular DHE fluorescence intensity. Data are represented as mean ± SD. * p

    Article Snippet: The solution of Pae-NPs was kept at room temperature.

    Techniques: Fluorescence

    Dose-dependent cytotoxicity of Pae and Pae-NPs against ( A ) A549 and ( B ) NCI-H1975 cells. Notes: Data are presented as mean ± SD (n=3). * p

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Dose-dependent cytotoxicity of Pae and Pae-NPs against ( A ) A549 and ( B ) NCI-H1975 cells. Notes: Data are presented as mean ± SD (n=3). * p

    Article Snippet: The solution of Pae-NPs was kept at room temperature.

    Techniques:

    Effects of Pae and Pae-NPs on apoptosis in A549 cells without ( A ) or with ( B ) the presence of NAC. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; NAC, N-acetylcysteine; Con, control; FITC, fluorescein isothiocyanate; PI, propidium iodide.

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Effects of Pae and Pae-NPs on apoptosis in A549 cells without ( A ) or with ( B ) the presence of NAC. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; NAC, N-acetylcysteine; Con, control; FITC, fluorescein isothiocyanate; PI, propidium iodide.

    Article Snippet: The solution of Pae-NPs was kept at room temperature.

    Techniques:

    Characterization of Pae-NPs. Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Characterization of Pae-NPs. Notes: ( A ) Size distribution of Pae-NPs measured by DLS. ( B ) Size changes of Pae-NPs at room temperature. ( C ) Cumulative release profile of Pae-NPs. Abbreviations: Pae-NPs, Pae-loaded nanoparticles; DLS, dynamic light scattering.

    Article Snippet: Drug loading content (DLC) and encapsulation efficiency (EE) High-performance liquid chromatography (HPLC) was used to analyze the loading efficiency of Pae-NPs (Shimadzu LC-10AD; Shimadzu, Japan).

    Techniques:

    Effects of free Pae or Pae-NPs on the expression of Akt pathway proteins in A549 cells. Notes: ( A ) Blots of p-Akt and Akt from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Effects of free Pae or Pae-NPs on the expression of Akt pathway proteins in A549 cells. Notes: ( A ) Blots of p-Akt and Akt from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Article Snippet: Drug loading content (DLC) and encapsulation efficiency (EE) High-performance liquid chromatography (HPLC) was used to analyze the loading efficiency of Pae-NPs (Shimadzu LC-10AD; Shimadzu, Japan).

    Techniques: Expressing, Standard Deviation

    Effect of free Pae or Pae-NPs on the expression of apoptotic proteins in A549 cells. Notes: ( A ) Blots of Bcl-XL, Bax, and Bcl-2 from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Effect of free Pae or Pae-NPs on the expression of apoptotic proteins in A549 cells. Notes: ( A ) Blots of Bcl-XL, Bax, and Bcl-2 from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Article Snippet: Drug loading content (DLC) and encapsulation efficiency (EE) High-performance liquid chromatography (HPLC) was used to analyze the loading efficiency of Pae-NPs (Shimadzu LC-10AD; Shimadzu, Japan).

    Techniques: Expressing, Standard Deviation

    Cellular uptake of coumarin-6-loaded fluorescent Pae-NPs by cancer cells. Notes: ( A , B ) Representative microscopic images of A549 cells incubated with coumarin-6-loaded Pae-NPs for 2 hours; ( A ) bright fields with higher magnification; ( B ) fluorescent fields with higher magnification. ( C ) Cellular uptake efficiency of Pae-NPs by A549 and NCI-H1975 cells. Data are presented as mean ± SD (n=3). ** p

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Cellular uptake of coumarin-6-loaded fluorescent Pae-NPs by cancer cells. Notes: ( A , B ) Representative microscopic images of A549 cells incubated with coumarin-6-loaded Pae-NPs for 2 hours; ( A ) bright fields with higher magnification; ( B ) fluorescent fields with higher magnification. ( C ) Cellular uptake efficiency of Pae-NPs by A549 and NCI-H1975 cells. Data are presented as mean ± SD (n=3). ** p

    Article Snippet: Drug loading content (DLC) and encapsulation efficiency (EE) High-performance liquid chromatography (HPLC) was used to analyze the loading efficiency of Pae-NPs (Shimadzu LC-10AD; Shimadzu, Japan).

    Techniques: Incubation

    In vivo antitumor efficacy of Pae-NPs. Notes: The mice were intravenously administered with PBS, E-NPs, free Pae, or Pae-NPs every 2 days for three consecutive injections. ( A ) Growth inhibition study in the A549 xenograft model. ( B ) Body weight changes for the tumor bearing mice after various formulations were given to mice on the indicated days. Data are represented as mean ± SD (n=6). * p

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: In vivo antitumor efficacy of Pae-NPs. Notes: The mice were intravenously administered with PBS, E-NPs, free Pae, or Pae-NPs every 2 days for three consecutive injections. ( A ) Growth inhibition study in the A549 xenograft model. ( B ) Body weight changes for the tumor bearing mice after various formulations were given to mice on the indicated days. Data are represented as mean ± SD (n=6). * p

    Article Snippet: Drug loading content (DLC) and encapsulation efficiency (EE) High-performance liquid chromatography (HPLC) was used to analyze the loading efficiency of Pae-NPs (Shimadzu LC-10AD; Shimadzu, Japan).

    Techniques: In Vivo, Mouse Assay, Inhibition

    Effect of free Pae or Pae-NPs on the expression of caspase-3 and cleaved caspase-3 proteins in A549 cells. Notes: ( A ) Blots of caspase-3 and pro-caspase-3 from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Effect of free Pae or Pae-NPs on the expression of caspase-3 and cleaved caspase-3 proteins in A549 cells. Notes: ( A ) Blots of caspase-3 and pro-caspase-3 from cells treated with different agents. ( B ) Semi-quantification of protein expression. Data are represented as mean ± SD. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; SD, standard deviation; NAC, N-acetylcysteine; Con, control.

    Article Snippet: Drug loading content (DLC) and encapsulation efficiency (EE) High-performance liquid chromatography (HPLC) was used to analyze the loading efficiency of Pae-NPs (Shimadzu LC-10AD; Shimadzu, Japan).

    Techniques: Expressing, Standard Deviation

    Detection of intracellular ROS. Notes: ( A ) DHE fluorescence images of A549 cells treated with different agents (control; E-NPs; Pae [50 μg/mL]; Pae-NPs [at an equivalent dose of 50 μg/mL]). ( B ) Intracellular DHE fluorescence intensity. Data are represented as mean ± SD. * p

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Detection of intracellular ROS. Notes: ( A ) DHE fluorescence images of A549 cells treated with different agents (control; E-NPs; Pae [50 μg/mL]; Pae-NPs [at an equivalent dose of 50 μg/mL]). ( B ) Intracellular DHE fluorescence intensity. Data are represented as mean ± SD. * p

    Article Snippet: Drug loading content (DLC) and encapsulation efficiency (EE) High-performance liquid chromatography (HPLC) was used to analyze the loading efficiency of Pae-NPs (Shimadzu LC-10AD; Shimadzu, Japan).

    Techniques: Fluorescence

    Dose-dependent cytotoxicity of Pae and Pae-NPs against ( A ) A549 and ( B ) NCI-H1975 cells. Notes: Data are presented as mean ± SD (n=3). * p

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Dose-dependent cytotoxicity of Pae and Pae-NPs against ( A ) A549 and ( B ) NCI-H1975 cells. Notes: Data are presented as mean ± SD (n=3). * p

    Article Snippet: Drug loading content (DLC) and encapsulation efficiency (EE) High-performance liquid chromatography (HPLC) was used to analyze the loading efficiency of Pae-NPs (Shimadzu LC-10AD; Shimadzu, Japan).

    Techniques:

    Effects of Pae and Pae-NPs on apoptosis in A549 cells without ( A ) or with ( B ) the presence of NAC. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; NAC, N-acetylcysteine; Con, control; FITC, fluorescein isothiocyanate; PI, propidium iodide.

    Journal: International Journal of Nanomedicine

    Article Title: Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

    doi: 10.2147/IJN.S143938

    Figure Lengend Snippet: Effects of Pae and Pae-NPs on apoptosis in A549 cells without ( A ) or with ( B ) the presence of NAC. Abbreviations: Pae, paeonol; Pae-NPs, Pae-loaded nanoparticles; E-NPs, empty nanoparticles; NAC, N-acetylcysteine; Con, control; FITC, fluorescein isothiocyanate; PI, propidium iodide.

    Article Snippet: Drug loading content (DLC) and encapsulation efficiency (EE) High-performance liquid chromatography (HPLC) was used to analyze the loading efficiency of Pae-NPs (Shimadzu LC-10AD; Shimadzu, Japan).

    Techniques:

    Crystal structure of the GCN4-pAe tetramer

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Self-assembly of coiled-coil tetramers in the 1.40 ? structure of a leucine-zipper mutant

    doi: 10.1110/ps.062590807

    Figure Lengend Snippet: Crystal structure of the GCN4-pAe tetramer

    Article Snippet: The GCN4-pAe peptide was expressed in Escherichia coli strain BL21(DE3)/pLysS (Novagen) using a modified pET3a vector ( ).

    Techniques:

    Interfacial interactions between GCN4-pAe dimers. ( A ) Side view showing side-to-side aggregation of dimers. The side chains of residues 19–32 that form the dimer–dimer interface are displayed. ( B ) Helix-to-helix packing in the Leu-A20( d )–Leu-C30( g )–Val-D31( a ) layer. The 1.40 Å 2 F obs – F calc electron density map contoured at 1.5σ is shown with the refined molecular model. ( C ) Helix to helix packing in the Val-B24( a )–Leu-C27( d )–Ala-D28( e ) layer. ( D ) Helical wheel projection of residues 19–32 of the GCN4-pAe tetramer, depicting interdimer packing interactions (dotted lines).

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Self-assembly of coiled-coil tetramers in the 1.40 ? structure of a leucine-zipper mutant

    doi: 10.1110/ps.062590807

    Figure Lengend Snippet: Interfacial interactions between GCN4-pAe dimers. ( A ) Side view showing side-to-side aggregation of dimers. The side chains of residues 19–32 that form the dimer–dimer interface are displayed. ( B ) Helix-to-helix packing in the Leu-A20( d )–Leu-C30( g )–Val-D31( a ) layer. The 1.40 Å 2 F obs – F calc electron density map contoured at 1.5σ is shown with the refined molecular model. ( C ) Helix to helix packing in the Val-B24( a )–Leu-C27( d )–Ala-D28( e ) layer. ( D ) Helical wheel projection of residues 19–32 of the GCN4-pAe tetramer, depicting interdimer packing interactions (dotted lines).

    Article Snippet: The GCN4-pAe peptide was expressed in Escherichia coli strain BL21(DE3)/pLysS (Novagen) using a modified pET3a vector ( ).

    Techniques: