p7 phage Search Results


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  • 99
    Zymo Research concentrator 5 kit
    Concentrator 5 Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1907 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC a baumannii atcc 17978 srnas
    Chromosomal location of coding sequences, small RNAs and TSS of A. <t>baumannii</t> ).
    A Baumannii Atcc 17978 Srnas, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore pronase
    Chromosomal location of coding sequences, small RNAs and TSS of A. <t>baumannii</t> ).
    Pronase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5468 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher zero blunt topo pcr cloning kit
    Chromosomal location of coding sequences, small RNAs and TSS of A. <t>baumannii</t> ).
    Zero Blunt Topo Pcr Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher vector nti
    Chromosomal location of coding sequences, small RNAs and TSS of A. <t>baumannii</t> ).
    Vector Nti, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TranS1 e coli trans1 t1 phage resistant
    Chromosomal location of coding sequences, small RNAs and TSS of A. <t>baumannii</t> ).
    E Coli Trans1 T1 Phage Resistant, supplied by TranS1, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene e coli xl1 blue
    Chromosomal location of coding sequences, small RNAs and TSS of A. <t>baumannii</t> ).
    E Coli Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 1750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC pseudomonas aeruginosa
    Chromosomal location of coding sequences, small RNAs and TSS of A. <t>baumannii</t> ).
    Pseudomonas Aeruginosa, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dynabeads myone streptavidin t1 magnetic beads
    Chromosomal location of coding sequences, small RNAs and TSS of A. <t>baumannii</t> ).
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    92
    Stratagene pbluescript sk
    Cloning of full-length TRL-78 transcripts by RT–PCR. TRL-78 was purified in a denaturing 5% polyacrylamide gel and tailed with poly(A) using poly(A) polymerase. A full-length cDNA was synthesized with Superscript RT using the primer dTBam. The cDNA was then tailed with dG using terminal deoxynucleotidyl transferase and amplified by PCR with the primers dCBam and dTBam. The resulting ~120-bp PCR product was digested with Bam HI and cloned into the Bam HI site of <t>pBluescript.</t> Six independent clones were found to have the TRL-78 sequence, which is shown at the bottom. The TRL-78 coding sequence in the Varkud mtDNA Eco RI-3 (E-3) fragment and the TRL-64 sequence inserted in pV1-2 are shown for comparison. The post-transcriptionally added 3′ CC residues are indicated in italics. In further experiments using other cloning procedures, an additional 90 clones were found to have the same TRL-78 sequence, with none lacking the 14-nt sequence that is deleted in TRL-64 (see Results).
    Pbluescript Sk, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 8857 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Larova GmbH polymerase
    Cloning of full-length TRL-78 transcripts by RT–PCR. TRL-78 was purified in a denaturing 5% polyacrylamide gel and tailed with poly(A) using poly(A) polymerase. A full-length cDNA was synthesized with Superscript RT using the primer dTBam. The cDNA was then tailed with dG using terminal deoxynucleotidyl transferase and amplified by PCR with the primers dCBam and dTBam. The resulting ~120-bp PCR product was digested with Bam HI and cloned into the Bam HI site of <t>pBluescript.</t> Six independent clones were found to have the TRL-78 sequence, which is shown at the bottom. The TRL-78 coding sequence in the Varkud mtDNA Eco RI-3 (E-3) fragment and the TRL-64 sequence inserted in pV1-2 are shown for comparison. The post-transcriptionally added 3′ CC residues are indicated in italics. In further experiments using other cloning procedures, an additional 90 clones were found to have the same TRL-78 sequence, with none lacking the 14-nt sequence that is deleted in TRL-64 (see Results).
    Polymerase, supplied by Larova GmbH, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATUM genomic dna
    Cloning of full-length TRL-78 transcripts by RT–PCR. TRL-78 was purified in a denaturing 5% polyacrylamide gel and tailed with poly(A) using poly(A) polymerase. A full-length cDNA was synthesized with Superscript RT using the primer dTBam. The cDNA was then tailed with dG using terminal deoxynucleotidyl transferase and amplified by PCR with the primers dCBam and dTBam. The resulting ~120-bp PCR product was digested with Bam HI and cloned into the Bam HI site of <t>pBluescript.</t> Six independent clones were found to have the TRL-78 sequence, which is shown at the bottom. The TRL-78 coding sequence in the Varkud mtDNA Eco RI-3 (E-3) fragment and the TRL-64 sequence inserted in pV1-2 are shown for comparison. The post-transcriptionally added 3′ CC residues are indicated in italics. In further experiments using other cloning procedures, an additional 90 clones were found to have the same TRL-78 sequence, with none lacking the 14-nt sequence that is deleted in TRL-64 (see Results).
    Genomic Dna, supplied by ATUM, used in various techniques. Bioz Stars score: 94/100, based on 2613 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher pcdna3 1 based plasmid
    Cloning of full-length TRL-78 transcripts by RT–PCR. TRL-78 was purified in a denaturing 5% polyacrylamide gel and tailed with poly(A) using poly(A) polymerase. A full-length cDNA was synthesized with Superscript RT using the primer dTBam. The cDNA was then tailed with dG using terminal deoxynucleotidyl transferase and amplified by PCR with the primers dCBam and dTBam. The resulting ~120-bp PCR product was digested with Bam HI and cloned into the Bam HI site of <t>pBluescript.</t> Six independent clones were found to have the TRL-78 sequence, which is shown at the bottom. The TRL-78 coding sequence in the Varkud mtDNA Eco RI-3 (E-3) fragment and the TRL-64 sequence inserted in pV1-2 are shown for comparison. The post-transcriptionally added 3′ CC residues are indicated in italics. In further experiments using other cloning procedures, an additional 90 clones were found to have the same TRL-78 sequence, with none lacking the 14-nt sequence that is deleted in TRL-64 (see Results).
    Pcdna3 1 Based Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher ecl detection system
    Cloning of full-length TRL-78 transcripts by RT–PCR. TRL-78 was purified in a denaturing 5% polyacrylamide gel and tailed with poly(A) using poly(A) polymerase. A full-length cDNA was synthesized with Superscript RT using the primer dTBam. The cDNA was then tailed with dG using terminal deoxynucleotidyl transferase and amplified by PCR with the primers dCBam and dTBam. The resulting ~120-bp PCR product was digested with Bam HI and cloned into the Bam HI site of <t>pBluescript.</t> Six independent clones were found to have the TRL-78 sequence, which is shown at the bottom. The TRL-78 coding sequence in the Varkud mtDNA Eco RI-3 (E-3) fragment and the TRL-64 sequence inserted in pV1-2 are shown for comparison. The post-transcriptionally added 3′ CC residues are indicated in italics. In further experiments using other cloning procedures, an additional 90 clones were found to have the same TRL-78 sequence, with none lacking the 14-nt sequence that is deleted in TRL-64 (see Results).
    Ecl Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2938 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore coomassie brilliant blue
    (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a <t>Coomassie</t> blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.
    Coomassie Brilliant Blue, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies bravo liquid handling system
    (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a <t>Coomassie</t> blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.
    Bravo Liquid Handling System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polyvinylidene difluoride membrane
    (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a <t>Coomassie</t> blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.
    Polyvinylidene Difluoride Membrane, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 26890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher topo ta cloning
    (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a <t>Coomassie</t> blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.
    Topo Ta Cloning, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t7 rna polymerase
    (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a <t>Coomassie</t> blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.
    T7 Rna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 10131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher t7 rna polymerase
    (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a <t>Coomassie</t> blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.
    T7 Rna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sp6 rna polymerase
    (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a <t>Coomassie</t> blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.
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    Agilent technologies multi site directed mutagenesis
    (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a <t>Coomassie</t> blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.
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    Pacific Biosciences phage like elements pacbio sequencing
    (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a <t>Coomassie</t> blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.
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    Bioedit Company dna sequences
    (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a <t>Coomassie</t> blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.
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    Image Search Results


    Chromosomal location of coding sequences, small RNAs and TSS of A. baumannii ).

    Journal: Nucleic Acids Research

    Article Title: The primary transcriptome, small RNAs and regulation of antimicrobial resistance in Acinetobacter baumannii ATCC 17978

    doi: 10.1093/nar/gky603

    Figure Lengend Snippet: Chromosomal location of coding sequences, small RNAs and TSS of A. baumannii ).

    Article Snippet: The group I family of A. baumannii ATCC 17978 sRNAs corresponds to the ‘C4-similar’ group in A. baumannii AB5075, reported previously to share regions of homology with bacteriophage P1 and P7 ( ).

    Techniques:

    sRNA conservation in representative members of the order Pseudomonadales. Genomes of multiple Acinetobacter species, Pseudomonas species, and Moraxella catarrhalis were compared using GLSEARCH. The colour scale shows the percentage sequence identity of the 110 sRNAs compared to the reference sequence from A. baumannii ATCC 17978.

    Journal: Nucleic Acids Research

    Article Title: The primary transcriptome, small RNAs and regulation of antimicrobial resistance in Acinetobacter baumannii ATCC 17978

    doi: 10.1093/nar/gky603

    Figure Lengend Snippet: sRNA conservation in representative members of the order Pseudomonadales. Genomes of multiple Acinetobacter species, Pseudomonas species, and Moraxella catarrhalis were compared using GLSEARCH. The colour scale shows the percentage sequence identity of the 110 sRNAs compared to the reference sequence from A. baumannii ATCC 17978.

    Article Snippet: The group I family of A. baumannii ATCC 17978 sRNAs corresponds to the ‘C4-similar’ group in A. baumannii AB5075, reported previously to share regions of homology with bacteriophage P1 and P7 ( ).

    Techniques: Sequencing

    Characterization of TSS. (A) Schematic explaining TSS categories. P: Primary TSS, S: Secondary TSS, I: Internal TSS, A: Antisense TSS, O: Orphan TSS. (B) Pie chart showing the number of TSS per category. (C) Histogram showing the number and length of 5′ UTRs of primary (red) and secondary (black) TSS. The inset illustrates the frequency of occurrence of nucleotides around the TSS. (D) Meme-derived motifs showing −35 and −10 region of A. baumannii ATCC 17978 promoters.

    Journal: Nucleic Acids Research

    Article Title: The primary transcriptome, small RNAs and regulation of antimicrobial resistance in Acinetobacter baumannii ATCC 17978

    doi: 10.1093/nar/gky603

    Figure Lengend Snippet: Characterization of TSS. (A) Schematic explaining TSS categories. P: Primary TSS, S: Secondary TSS, I: Internal TSS, A: Antisense TSS, O: Orphan TSS. (B) Pie chart showing the number of TSS per category. (C) Histogram showing the number and length of 5′ UTRs of primary (red) and secondary (black) TSS. The inset illustrates the frequency of occurrence of nucleotides around the TSS. (D) Meme-derived motifs showing −35 and −10 region of A. baumannii ATCC 17978 promoters.

    Article Snippet: The group I family of A. baumannii ATCC 17978 sRNAs corresponds to the ‘C4-similar’ group in A. baumannii AB5075, reported previously to share regions of homology with bacteriophage P1 and P7 ( ).

    Techniques: Derivative Assay

    sRNA in A. baumannii ATCC 17978. ( A ) Normalized, mapped sequence reads from RNA-seq show the expression of sRNAs 17, 37, 75, 76, 77, 84, 99 and 100 (yellow arrows). Curved arrows depict TSS identified in this study and lollipop structures are predicted rho-independent terminators. Northern blotting of selected sRNAs are shown to the right. RNA was isolated from ESP and five μg of total RNA was loaded per lane. The sRNA sizes below the individual blots have been predicted from dRNA-seq data. ( B ) Sequence alignment of Group I and Group III sRNAs created with the Geneious Software (v. 8.1.8); colored bases indicate conservation in at least 50% of aligned sequences (A, red; C, blue; G, yellow; T, green). The riboprobes used in Northern blotting are depicted as black bars atop the sRNA alignments.

    Journal: Nucleic Acids Research

    Article Title: The primary transcriptome, small RNAs and regulation of antimicrobial resistance in Acinetobacter baumannii ATCC 17978

    doi: 10.1093/nar/gky603

    Figure Lengend Snippet: sRNA in A. baumannii ATCC 17978. ( A ) Normalized, mapped sequence reads from RNA-seq show the expression of sRNAs 17, 37, 75, 76, 77, 84, 99 and 100 (yellow arrows). Curved arrows depict TSS identified in this study and lollipop structures are predicted rho-independent terminators. Northern blotting of selected sRNAs are shown to the right. RNA was isolated from ESP and five μg of total RNA was loaded per lane. The sRNA sizes below the individual blots have been predicted from dRNA-seq data. ( B ) Sequence alignment of Group I and Group III sRNAs created with the Geneious Software (v. 8.1.8); colored bases indicate conservation in at least 50% of aligned sequences (A, red; C, blue; G, yellow; T, green). The riboprobes used in Northern blotting are depicted as black bars atop the sRNA alignments.

    Article Snippet: The group I family of A. baumannii ATCC 17978 sRNAs corresponds to the ‘C4-similar’ group in A. baumannii AB5075, reported previously to share regions of homology with bacteriophage P1 and P7 ( ).

    Techniques: Sequencing, RNA Sequencing Assay, Expressing, Northern Blot, Isolation, End-sequence Profiling, Software

    Cloning of full-length TRL-78 transcripts by RT–PCR. TRL-78 was purified in a denaturing 5% polyacrylamide gel and tailed with poly(A) using poly(A) polymerase. A full-length cDNA was synthesized with Superscript RT using the primer dTBam. The cDNA was then tailed with dG using terminal deoxynucleotidyl transferase and amplified by PCR with the primers dCBam and dTBam. The resulting ~120-bp PCR product was digested with Bam HI and cloned into the Bam HI site of pBluescript. Six independent clones were found to have the TRL-78 sequence, which is shown at the bottom. The TRL-78 coding sequence in the Varkud mtDNA Eco RI-3 (E-3) fragment and the TRL-64 sequence inserted in pV1-2 are shown for comparison. The post-transcriptionally added 3′ CC residues are indicated in italics. In further experiments using other cloning procedures, an additional 90 clones were found to have the same TRL-78 sequence, with none lacking the 14-nt sequence that is deleted in TRL-64 (see Results).

    Journal: Nucleic Acids Research

    Article Title: Characterization of an unusual tRNA-like sequence found inserted in a Neurospora retroplasmid

    doi:

    Figure Lengend Snippet: Cloning of full-length TRL-78 transcripts by RT–PCR. TRL-78 was purified in a denaturing 5% polyacrylamide gel and tailed with poly(A) using poly(A) polymerase. A full-length cDNA was synthesized with Superscript RT using the primer dTBam. The cDNA was then tailed with dG using terminal deoxynucleotidyl transferase and amplified by PCR with the primers dCBam and dTBam. The resulting ~120-bp PCR product was digested with Bam HI and cloned into the Bam HI site of pBluescript. Six independent clones were found to have the TRL-78 sequence, which is shown at the bottom. The TRL-78 coding sequence in the Varkud mtDNA Eco RI-3 (E-3) fragment and the TRL-64 sequence inserted in pV1-2 are shown for comparison. The post-transcriptionally added 3′ CC residues are indicated in italics. In further experiments using other cloning procedures, an additional 90 clones were found to have the same TRL-78 sequence, with none lacking the 14-nt sequence that is deleted in TRL-64 (see Results).

    Article Snippet: Plasmid p7-2 contains the TRL-78 sequence from Varkud cloned similarly in pBluescript SK(+) (Stratagene).

    Techniques: Clone Assay, Reverse Transcription Polymerase Chain Reaction, Purification, Synthesized, Amplification, Polymerase Chain Reaction, Sequencing

    (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a Coomassie blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.

    Journal: Journal of Virology

    Article Title: The Small Viral Membrane-Associated Protein P32 Is Involved in Bacteriophage PRD1 DNA Entry

    doi: 10.1128/JVI.76.10.4866-4872.2002

    Figure Lengend Snippet: (A) An electron micrograph of thin-sectioned DS88 cells infected with PRD1 [ lac Zα]-9. The image, obtained 55 min postinfection, shows a large number of peripheral filled phage particles (bar, 500 nm). (B) Protein composition of PRD1 wt and P32-deficient mutant particles is shown in a Coomassie blue-stained Tricine SDS-polyacrylamide gel. In the mutant phage preparation the only protein missing is the 5.4-kDa P32 (marked by an arrow). Molecular weight markers and some PRD1 proteins are indicated on the left and right, respectively.

    Article Snippet: The proteins were either stained with Coomassie brilliant blue or transferred from the gel onto a polyvinylidene difluoride membrane (Millipore) and visualized with the ECL detection system (Pierce) using horseradish peroxidase-conjugated swine anti-rabbit immunoglobulin Gs (IgGs) (DAKO) or peroxidase-conjugated horse anti-mouse IgGs (Vector) as secondary antibodies.

    Techniques: Infection, Mutagenesis, Staining, Phage Preparation, Molecular Weight