Journal: Purinergic Signalling

Article Title: Bone turnover is altered in transgenic rats overexpressing the P2Y2 purinergic receptor

doi: 10.1007/s11302-017-9582-3

Figure Lengend Snippet: a Fold change in expression of P2Y2 mRNA in brain and lung tissue (n = 3) as well as calvarial osteoblasts (n = 4) of P2Y2R-Tg rats relative to Wt (n = 3 (brain and lung); n = 6 (calvarial osteoblasts)). b P2Y2R protein expression in calvarial osteoblasts from WT (n = 4) and P2Y2R-Tg (n = 4) rats as measured by Western blotting. All bands are normalized to histone H3 expression, and mean expression in Wt cells is set at 100%. Lower panel shows membrane images of each individual experiment. Error bars represent SEM. TG P2Y2R transgenic, Wt wild-type

Article Snippet: Settings were 25 °C for 10 min, then 37 °C for 120 min, then 85 °C for 5 min, and finally 4 °C until use or storage. qPCR was performed using a 2-μl cDNA template and the Applied Biosystems TaqMan© Universal PCR Master Mix plus relevant TaqMan probes (rat P2Y2, #Rn02070661_s1, and rat HPRT1, Rn01527840_m1) according to the manufacturer’s recommendations in 20 μl total volume.

Techniques: Expressing, Western Blot, Transgenic Assay

Journal: The Journal of Physiology

Article Title: Large‐conductance Ca 2+ ‐activated K + channel activation by apical P2Y receptor agonists requires hydrocortisone in differentiated airway epithelium

doi: 10.1113/JP274200

Figure Lengend Snippet: A, qRT‐PCR analysis of P2YR mRNAs in control and HC0 monolayers after 8 days of differentiation (n = 6). Statistical significance (*, P < 0.05) represents differences between relative expression of mRNAs in control and HC0 cells determined by Student's t tests. B, representative I SC traces of control and HC0 (inset) epithelia stimulated with 20 μm UTP then 20 μm ATP‐γ‐S (n = 6). C and D, representative I SC traces of control (C) and HC0 (D) epithelia stimulated with 20 μm ATP‐γ‐S then 20 μm UTP (n = 6). E and F, histogram summarizing the effect of UTP and ATP‐γ‐S on I SC in control (E) and HC0 (F) epithelia. Initial stimulant is represented as left bar in each pair. Statistical significance (P < 0.05) between initial and post stimuli marked by (*), between primary stimuli marked by (†), between post stimuli marked by (○), between UTP bars marked by (◊), between ATP‐γ‐S bars marked by (#). G, Western blot analysis of P2Y2R (100%, 37.2%, 66.1%, 56.1%) (upper) and P2Y4R (100%, 64.0%, 61.8%, 61.7%) (middle) in day 8 and day 24 control and HC0 epithelia, respectively. Protein abundance is normalized to β‐actin (lower) for each sample and expressed as a percentage of control at day 8. H, Immunocytochemistry showing the co‐localization of P2Y2 and BKα in the cilia of control epithelia. P2Y4 is only expressed in the apical membrane and not within the cilia.

Article Snippet: P2RY2 , Hs04176264_s1 , 3–3.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Immunocytochemistry

Journal: The Journal of Physiology

Article Title: Large‐conductance Ca 2+ ‐activated K + channel activation by apical P2Y receptor agonists requires hydrocortisone in differentiated airway epithelium

doi: 10.1113/JP274200

Figure Lengend Snippet: TaqMan probes used in QRT‐PCR assays

Article Snippet: P2RY2 , Hs04176264_s1 , 3–3.

Techniques: