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    Alomone Labs p2x7r
    ERM activation is specifically induced by <t>P2X7R</t> stimulation. A , Neuro2a cells were pretreated with or without a pharmacological inhibitor of P2X7R (A438079) at 37 °C for 1 h and stimulated or not with 1 m m Bz-ATP for 10 min. Proteins from whole
    P2x7r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x7r/product/Alomone Labs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2x7r - by Bioz Stars, 2022-10
    96/100 stars
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    92
    Tocris p2x7r
    The knockdown of <t>P2X7R</t> in DRG reduced the mechanical hyperalgesia induced by CFA. (A) Antisense ODN (AS) for P2X7R (30 mg/5 ml daily for 4 days) administered in DRG before intraplantar administration of CFA significantly reduced the mechanical hyperalgesia assessed 6 h later (One-way ANOVA and Bonferroni post-test; P
    P2x7r, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x7r/product/Tocris
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2x7r - by Bioz Stars, 2022-10
    92/100 stars
      Buy from Supplier

    Image Search Results


    ERM activation is specifically induced by P2X7R stimulation. A , Neuro2a cells were pretreated with or without a pharmacological inhibitor of P2X7R (A438079) at 37 °C for 1 h and stimulated or not with 1 m m Bz-ATP for 10 min. Proteins from whole

    Journal: The Journal of Biological Chemistry

    Article Title: Ezrin/Radixin/Moesin Are Required for the Purinergic P2X7 Receptor (P2X7R)-dependent Processing of the Amyloid Precursor Protein *

    doi: 10.1074/jbc.M112.400010

    Figure Lengend Snippet: ERM activation is specifically induced by P2X7R stimulation. A , Neuro2a cells were pretreated with or without a pharmacological inhibitor of P2X7R (A438079) at 37 °C for 1 h and stimulated or not with 1 m m Bz-ATP for 10 min. Proteins from whole

    Article Snippet: This method relies on the use of two primary antibodies from two different species reacting with ezrin or P2X7R.

    Techniques: Activation Assay

    P2X7R-dependent cation channel opening and non-selective pore formation are not or are mildly modulated by ERM, respectively. A , Neuro2a cells were transfected with 3 μ m control or ERM-specific siRNAs. After 48 h, cells were lysed, and proteins

    Journal: The Journal of Biological Chemistry

    Article Title: Ezrin/Radixin/Moesin Are Required for the Purinergic P2X7 Receptor (P2X7R)-dependent Processing of the Amyloid Precursor Protein *

    doi: 10.1074/jbc.M112.400010

    Figure Lengend Snippet: P2X7R-dependent cation channel opening and non-selective pore formation are not or are mildly modulated by ERM, respectively. A , Neuro2a cells were transfected with 3 μ m control or ERM-specific siRNAs. After 48 h, cells were lysed, and proteins

    Article Snippet: This method relies on the use of two primary antibodies from two different species reacting with ezrin or P2X7R.

    Techniques: Transfection

    ERM are involved in P2X7R-dependent sAPPα shedding. A , Neuro2a cells were transfected with 3 μ m control or ERM siRNAs. After 48 h, cells were stimulated or not with 1 m m Bz-ATP for 10 min. Proteins from cell lysates were analyzed by Western

    Journal: The Journal of Biological Chemistry

    Article Title: Ezrin/Radixin/Moesin Are Required for the Purinergic P2X7 Receptor (P2X7R)-dependent Processing of the Amyloid Precursor Protein *

    doi: 10.1074/jbc.M112.400010

    Figure Lengend Snippet: ERM are involved in P2X7R-dependent sAPPα shedding. A , Neuro2a cells were transfected with 3 μ m control or ERM siRNAs. After 48 h, cells were stimulated or not with 1 m m Bz-ATP for 10 min. Proteins from cell lysates were analyzed by Western

    Article Snippet: This method relies on the use of two primary antibodies from two different species reacting with ezrin or P2X7R.

    Techniques: Transfection, Western Blot

    P2X7R-dependent biochemical pathways involved in ERM activation. A and B , Neuro2a cells were pretreated with or without 500 n m fasudil, a pharmacological inhibitor of Rho kinases, at 37 °C for 1 h and stimulated with 1 m m Bz-ATP for 10 min. A

    Journal: The Journal of Biological Chemistry

    Article Title: Ezrin/Radixin/Moesin Are Required for the Purinergic P2X7 Receptor (P2X7R)-dependent Processing of the Amyloid Precursor Protein *

    doi: 10.1074/jbc.M112.400010

    Figure Lengend Snippet: P2X7R-dependent biochemical pathways involved in ERM activation. A and B , Neuro2a cells were pretreated with or without 500 n m fasudil, a pharmacological inhibitor of Rho kinases, at 37 °C for 1 h and stimulated with 1 m m Bz-ATP for 10 min. A

    Article Snippet: This method relies on the use of two primary antibodies from two different species reacting with ezrin or P2X7R.

    Techniques: Activation Assay

    Schematic representation of the biochemical pathways involved in P2X7R-dependent proteolytic cleavage of APP. P2X7R stimulation by Bz-ATP triggers Ca 2+ influx and activates the Rho kinase and the MAP kinases ERK1/2 and JNK. These Ser/Thr kinases directly

    Journal: The Journal of Biological Chemistry

    Article Title: Ezrin/Radixin/Moesin Are Required for the Purinergic P2X7 Receptor (P2X7R)-dependent Processing of the Amyloid Precursor Protein *

    doi: 10.1074/jbc.M112.400010

    Figure Lengend Snippet: Schematic representation of the biochemical pathways involved in P2X7R-dependent proteolytic cleavage of APP. P2X7R stimulation by Bz-ATP triggers Ca 2+ influx and activates the Rho kinase and the MAP kinases ERK1/2 and JNK. These Ser/Thr kinases directly

    Article Snippet: This method relies on the use of two primary antibodies from two different species reacting with ezrin or P2X7R.

    Techniques:

    Ezrin interacts with P2X7R. Neuro2a cells were seeded on Lab-Tek slides and incubated for 48 h at 37 °C. A , cells were incubated without Basal ) or with ( Stimulated ) 1 m m Bz-ATP for 10 min, fixed with 4% paraformaldehyde, permeabilized, and treated

    Journal: The Journal of Biological Chemistry

    Article Title: Ezrin/Radixin/Moesin Are Required for the Purinergic P2X7 Receptor (P2X7R)-dependent Processing of the Amyloid Precursor Protein *

    doi: 10.1074/jbc.M112.400010

    Figure Lengend Snippet: Ezrin interacts with P2X7R. Neuro2a cells were seeded on Lab-Tek slides and incubated for 48 h at 37 °C. A , cells were incubated without Basal ) or with ( Stimulated ) 1 m m Bz-ATP for 10 min, fixed with 4% paraformaldehyde, permeabilized, and treated

    Article Snippet: This method relies on the use of two primary antibodies from two different species reacting with ezrin or P2X7R.

    Techniques: Incubation

    The knockdown of P2X7R in DRG reduced the mechanical hyperalgesia induced by CFA. (A) Antisense ODN (AS) for P2X7R (30 mg/5 ml daily for 4 days) administered in DRG before intraplantar administration of CFA significantly reduced the mechanical hyperalgesia assessed 6 h later (One-way ANOVA and Bonferroni post-test; P

    Journal: Frontiers in Physiology

    Article Title: Peripheral Inflammatory Hyperalgesia Depends on P2X7 Receptors in Satellite Glial Cells

    doi: 10.3389/fphys.2020.00473

    Figure Lengend Snippet: The knockdown of P2X7R in DRG reduced the mechanical hyperalgesia induced by CFA. (A) Antisense ODN (AS) for P2X7R (30 mg/5 ml daily for 4 days) administered in DRG before intraplantar administration of CFA significantly reduced the mechanical hyperalgesia assessed 6 h later (One-way ANOVA and Bonferroni post-test; P

    Article Snippet: Other studies, mostly in neuropathic pain-like behavior models, also detected upregulation of P2X receptors in trigeminal SGCs ( ) and specifically upregulation of P2X7R in spinal microglia ( ; ; ), and Schwan cells ( ).

    Techniques:

    Selective blockade of P2X7R expressed on satellite glial cells reduced the mechanical hyperalgesia induced by CFA, Carrageenan or IL-1b, but not PGE2. (A) A-740003 (0.01, 0.1, and 1.0 mM/5 μL) administered in DRG (i.gl.) immediately before intraplantar administration of CFA significantly reduced the mechanical hyperalgesia evaluated 6 h later (One-way ANOVA and Bonferroni post-test; P

    Journal: Frontiers in Physiology

    Article Title: Peripheral Inflammatory Hyperalgesia Depends on P2X7 Receptors in Satellite Glial Cells

    doi: 10.3389/fphys.2020.00473

    Figure Lengend Snippet: Selective blockade of P2X7R expressed on satellite glial cells reduced the mechanical hyperalgesia induced by CFA, Carrageenan or IL-1b, but not PGE2. (A) A-740003 (0.01, 0.1, and 1.0 mM/5 μL) administered in DRG (i.gl.) immediately before intraplantar administration of CFA significantly reduced the mechanical hyperalgesia evaluated 6 h later (One-way ANOVA and Bonferroni post-test; P

    Article Snippet: Other studies, mostly in neuropathic pain-like behavior models, also detected upregulation of P2X receptors in trigeminal SGCs ( ) and specifically upregulation of P2X7R in spinal microglia ( ; ; ), and Schwan cells ( ).

    Techniques:

    P2X7R are expressed by SGCs in DRG and can be functionally activated and blocked in culture. (A) Co-staining of glutamine synthetase (GS, yellow) and P2X7R (green) in DRG sections of CFA-treated animals. In non-inflammatory condition, expression of P2X7R is very low and hardly detected by DRG immunohistochemistry. The merged picture shows co-localization of P2X7R and glutamine synthetase in SGCs surrounding neurons, and cell nuclei were staining with DAPI (blue). (B) BzATP (100 μM) induced intracellular calcium surge in cultured SGCs (colored artificially) but not in neurons ( n ). (C) Pretreatment with A-740003 (1 μM, green line) blocked the BzATP-induced intracellular calcium surge (gray line). (D) Maximum values of ΔF/F 0 for both response curves showed in (C) . Data are expressed as mean ± SEM for n = 5 cultures/group, 20–40 SGCs sampled per culture. Symbol *** indicates statistical difference between the response peaks (Unpaired t -test; P

    Journal: Frontiers in Physiology

    Article Title: Peripheral Inflammatory Hyperalgesia Depends on P2X7 Receptors in Satellite Glial Cells

    doi: 10.3389/fphys.2020.00473

    Figure Lengend Snippet: P2X7R are expressed by SGCs in DRG and can be functionally activated and blocked in culture. (A) Co-staining of glutamine synthetase (GS, yellow) and P2X7R (green) in DRG sections of CFA-treated animals. In non-inflammatory condition, expression of P2X7R is very low and hardly detected by DRG immunohistochemistry. The merged picture shows co-localization of P2X7R and glutamine synthetase in SGCs surrounding neurons, and cell nuclei were staining with DAPI (blue). (B) BzATP (100 μM) induced intracellular calcium surge in cultured SGCs (colored artificially) but not in neurons ( n ). (C) Pretreatment with A-740003 (1 μM, green line) blocked the BzATP-induced intracellular calcium surge (gray line). (D) Maximum values of ΔF/F 0 for both response curves showed in (C) . Data are expressed as mean ± SEM for n = 5 cultures/group, 20–40 SGCs sampled per culture. Symbol *** indicates statistical difference between the response peaks (Unpaired t -test; P

    Article Snippet: Other studies, mostly in neuropathic pain-like behavior models, also detected upregulation of P2X receptors in trigeminal SGCs ( ) and specifically upregulation of P2X7R in spinal microglia ( ; ; ), and Schwan cells ( ).

    Techniques: Staining, Expressing, Immunohistochemistry, Cell Culture

    Activation of P2X7R increased the release but not the synthesis of IL-1β in satellite glial cells after an inflammatory stimulus. (A) Co-staining of glutamine synthetase (GS, yellow) and IL-1β (red) in DRG sections. In non-inflammatory condition, expression of IL-1β is very low and hardly detected by DRG immunohistochemistry. The merged picture shows co-localization of IL-1β and glutamine synthetase in SGCs surrounding neurons, and cell nuclei were staining with DAPI (blue). (B) SGC-enriched cultures were stimulated with LPS (1 μg/mL) for 24 h and then incubated with A-740003 (1 μM) for 15 min followed by treatment with BzATP (100 μM) for 30 min. LPS induced the release of IL-1β in the supernatant of SGCs cultures, but BzATP significantly increased the release of IL-1β by SGCs, which was prevented with A-740003. (C) IL-1β synthesis is increased in vitro only in LPS-stimulated cultures, regardless of P2X7R activation or blockade, and (D) in vivo after 6 h of the CFA inflammation in the ipsilateral hind paw. IL-1β relative expression in the contralateral paw had no difference from the saline group. Data are expressed as mean ± SEM. Symbol # indicates statistical difference comparing to control groups and *** indicates statistical difference comparing to LPS-stimulated groups [One-way ANOVA and Bonferroni post-test; P

    Journal: Frontiers in Physiology

    Article Title: Peripheral Inflammatory Hyperalgesia Depends on P2X7 Receptors in Satellite Glial Cells

    doi: 10.3389/fphys.2020.00473

    Figure Lengend Snippet: Activation of P2X7R increased the release but not the synthesis of IL-1β in satellite glial cells after an inflammatory stimulus. (A) Co-staining of glutamine synthetase (GS, yellow) and IL-1β (red) in DRG sections. In non-inflammatory condition, expression of IL-1β is very low and hardly detected by DRG immunohistochemistry. The merged picture shows co-localization of IL-1β and glutamine synthetase in SGCs surrounding neurons, and cell nuclei were staining with DAPI (blue). (B) SGC-enriched cultures were stimulated with LPS (1 μg/mL) for 24 h and then incubated with A-740003 (1 μM) for 15 min followed by treatment with BzATP (100 μM) for 30 min. LPS induced the release of IL-1β in the supernatant of SGCs cultures, but BzATP significantly increased the release of IL-1β by SGCs, which was prevented with A-740003. (C) IL-1β synthesis is increased in vitro only in LPS-stimulated cultures, regardless of P2X7R activation or blockade, and (D) in vivo after 6 h of the CFA inflammation in the ipsilateral hind paw. IL-1β relative expression in the contralateral paw had no difference from the saline group. Data are expressed as mean ± SEM. Symbol # indicates statistical difference comparing to control groups and *** indicates statistical difference comparing to LPS-stimulated groups [One-way ANOVA and Bonferroni post-test; P

    Article Snippet: Other studies, mostly in neuropathic pain-like behavior models, also detected upregulation of P2X receptors in trigeminal SGCs ( ) and specifically upregulation of P2X7R in spinal microglia ( ; ; ), and Schwan cells ( ).

    Techniques: Activation Assay, Staining, Expressing, Immunohistochemistry, Incubation, In Vitro, In Vivo