Journal: The Journal of Neuroscience

Article Title: Peripheral Inflammation Sensitizes P2X Receptor-Mediated Responses in Rat Dorsal Root Ganglion Neurons

doi: 10.1523/JNEUROSCI.22-01-00093.2002

Figure Lengend Snippet: CFA treatment enhances P2X2 and P2X3 receptor expression. A , Western blots for P2X2 and P2X3 receptors from ganglia of control rats ( CON ) and rats 5 d after CFA treatment. Actin control for each sample was given. B , Mean density relative to control rats

Article Snippet: Thus, inflammation upregulates the P2X2 and P2X3 receptor expression in DRGs.

Techniques: Expressing, Western Blot

Journal: Neuropharmacology

Article Title: P2X receptor overexpression induced by soluble oligomers of amyloid beta peptide potentiates synaptic failure and neuronal dyshomeostasis in cellular models of Alzheimer’s disease

doi: 10.1016/j.neuropharm.2017.10.027

Figure Lengend Snippet: SO-Aβ induces changes in the mRNA expression levels of P2X subunits. A) Quantification of relative mRNA for P2X subunits at 12 h of treatment with Aβ (0.5 μM) revealed that the subunits P2X1, 2, 5 and 7 had a significant increase in their expression (P2X1: 1.25 ± 0.007, P = 0.0009; P2X2: 1.23 ± 0.06, P = 0.029; P2X5: 1.21 ± 0.06, P = 0.039; P2X7: 1.42 ± 0.06, P = 0.019; P2X3: 0.95 ± 0.09, P = 0.636; P2X4: 1.21 ± 0.07, P = 0.056; P2X6: 1.03 ± 0.22, P = 0.918). B) Quantification of the relative expression of P2X at 24 h of treatment (Aβ, 0.5 μM) showing that at this time point only P2X2 remained significantly increased (P2X2: 1.2 ± 0.04, P = 0.022; P2X1: 1.1 ± 0.06, P = 0.152; P2X3: 0.9 ± 0.08, P = 0.286; P2X4: 1.16 ± 0.07, P = 0.110; P2X5: 1.13 ± 0.05, P = 0.096; P2X6: 0.95 ± 0.15, P = 0.783; P2X7: 1.21 ± 0.13, P = 0.193), n = 5. *P

Article Snippet: The primary antibodies used were anti-PSD95 (Mouse monoclonal, 1:1000, Thermo Scientific, Waltham, MA, USA), anti-P2X2 and anti P2X7 (polyclonal, 1:1000, Alomone Labs, Israel) and anti-β-actin (mouse monoclonal, 1:1000, Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Expressing

Journal: Neuropharmacology

Article Title: P2X receptor overexpression induced by soluble oligomers of amyloid beta peptide potentiates synaptic failure and neuronal dyshomeostasis in cellular models of Alzheimer’s disease

doi: 10.1016/j.neuropharm.2017.10.027

Figure Lengend Snippet: P2X2R immunocytochemistry of hippocampal neurons treated with SO-Aβ. A-I) Representative image of hippocampal neuron (10 DIV) in control conditions stained with MAP-2 (red) and P2X2 receptor subunit (green, scale bar 20 μm). A-II) Magnification of control neuron with immunostaining for P2X2R (green). A-III) SO-Aβ treated neuron and staining in the same conditions as Aa. A-IV) Magnification of SO-Aβ treated neuron with immunostaining for P2X2R (green). B) Quantification of P2X2R immunoreactivity in SO-Aβ treated neurons with respect to control conditions (n = 4, * vs control condition). C-I) Representative image of a hippocampal neuron (10 DIV) in control conditions stained with MAP-2 (red) and P2X7 receptor subunit (green, scale bar 20 μm). C-II) Magnification of control neuron with immunostaining for P2X7R (green). C-III) SO-Aβ treated neuron and staining in the same conditions as Aa. C-IV) Magnification of SO-Aβ treated neuron with immunostaining for P2X7R (green). D) Quantification of P2X7R immunoreactivity in SO-Aβ treated neurons with respect to control conditions (n = 4).

Article Snippet: The primary antibodies used were anti-PSD95 (Mouse monoclonal, 1:1000, Thermo Scientific, Waltham, MA, USA), anti-P2X2 and anti P2X7 (polyclonal, 1:1000, Alomone Labs, Israel) and anti-β-actin (mouse monoclonal, 1:1000, Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Immunocytochemistry, Staining, Immunostaining

Journal: Development (Cambridge, England)

Article Title: Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium

doi: 10.1242/dev.107631

Figure Lengend Snippet: Fungiform taste buds are innervated by P2X2 + and PGP9.5 + nerve fibers, whereas ectopic taste buds form in lingual epithelium innervated only by PGP9.5 + fibers. (A) A fungiform taste bud is innervated by P2X2 + taste fibers, whereas ectopic taste buds (B)

Article Snippet: Primary antisera and dilutions: rat anti-K8 (Troma) (1:200; Developmental Studies Hybridoma Bank, University of Iowa, USA), rabbit anti-claudin 4 (1:250; 364800, Invitrogen), rabbit anti-NTPDase2 (1:3000; mN2-36L, CHUQ), rabbit anti-α-gustducin (1:1000; sc-395, Santa Cruz), rabbit anti-SNAP25 (1:8000; S9684, Sigma), rabbit anti-PLCβ2 (1:1000; sc-206, Santa Cruz), rabbit anti-NCAM (1:1000; AB5032, Millipore), goat anti-KCNQ1 (1:500; sc-10646, Santa Cruz), guinea pig anti-TRPM5 (1:1000; from Dr Emily Liman at USC), goat anti-CAR4 (1:1000; AF2414, R & D Systems), rabbit anti-K14 (1:3500; PRB-155P, Covance), guinea pig anti-K13 (1:500; BP5076, Acris Antibodies), goat anti-SOX2 (1:500; sc-17320, Santa Cruz), rabbit anti-SKN-1A (POU2F3) (1:500; sc-330, Santa Cruz), rabbit anti-P2X2 (1:500; APR-003, Alomone Labs), rabbit anti-PGP9.5 (1:1000; 7863-0504, AbD Serotec), rabbit anti-β-galactosidase (1:1000; 55976, MP Biomedicals) and chicken anti-GFP (1:1000; GFP-1020, Aves Labs).

Techniques: