Journal: Cancer Science
Article Title: Solute carrier family 35 member F2 is indispensable for papillary thyroid carcinoma progression through activation of transforming growth factor‐β type I receptor/apoptosis signal‐regulating kinase 1/mitogen‐activated protein kinase signaling axis, et al. Solute carrier family 35 member F2 is indispensable for papillary thyroid carcinoma progression through activation of transforming growth factor‐β type I receptor/apoptosis signal‐regulating kinase 1/mitogen‐activated protein kinase signaling axis
Figure Lengend Snippet: Cyclin‐dependent kinases and cyclins are involved in solute carrier family 35 member F2 ( SLC 35F2) ‐ regulated cell cycle progression. A, Effects of SLC 35F2 knockout on cell cycle by FACS . B, Cell cycle distribution showed that inhibition of SLC 35F2 induced cell cycle arrest at G1 phase. C, Flow cytometry analysis of the cell cycle of SLC 35F2 overexpression and empty vector control cells. D, Ectopic expression of SLC 35F2 promoted G1/S phase transition and increased S‐phase proportion. E, F, Protein levels of CDK 2, CDK 4, CDK 6, Cyclin D1, Cyclin D3, p27 Kip1, p21 Waf1/Cip 1 and p18 INK 4C were detected by western blotting in indicated PTC cells. G, Relative mRNA expression levels of some cell cycle related genes analyzed by qPCR in SLC 35F2‐ KO group compared to control KTC ‐1 cells. Data were plotted relative to expression levels in control cells (* P
Article Snippet: The following antibodies were used to explore protein expression: anti‐SLC35F2 antibodies (AV43971, Sigma‐Aldrich), anti‐β‐actin (#4970), anti‐CDK2 (#2546), anti‐CDK4 (#12790), anti‐CDK6 (#13331), anti‐Cyclin D1 (#2978), anti‐Cyclin D3 (#2936), anti‐p27 Kip1 (#3686), anti‐p21 Waf1/Cip1 (#2947), anti‐p18 INK4C (#2896), anti‐ERK1/2 (#4695), anti‐p‐ERK1/2 (Thr202/Tyr204, #4370), anti‐JNK (#9252), anti‐p‐JNK (Thr183/Tyr185, #4668), anti‐p38 MAPK (#8690), anti‐p‐p38 MAPK (Thr180/Tyr182, #4511), anti‐ASK‐1 (#8662), anti‐phosphorylation of apoptosis signal‐regulating kinase 1 (p‐ASK‐1) (Thr845, #3765) (Cell Signaling Technology) and anti‐TGFBR1 (CY2905, Abways, China).
Techniques: Knock-Out, FACS, Inhibition, Flow Cytometry, Cytometry, Over Expression, Plasmid Preparation, Expressing, Sublimation, Western Blot, Real-time Polymerase Chain Reaction