p22 phox Search Results


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    • human p22 phox cyba  (OriGene)
    92
    OriGene human p22 phox cyba
    (A-C) Mouse constructs encoding EROS and gp91 <t>phox</t> were co-transfected into NIH3T3 (A) , COS-7 (B) and HEK293T (C) cell lines. gp91 phox expression was analysed by immunoblotting; arrow indicates gp91 phox band; ns: non-specific band. (D-F) gp91 phox and <t>p22</t> phox expression in HEK293T cells following transfection with the indicated human constructs. (G) Analysis of the stability of the different forms of gp91 phox (indicated by the arrows) following transfection in HEK293T cells in presence or absence of EROS and treatment with 10μg/mL cycloheximide. Actin and vinculin were used as loading control. (H) Stability of endogenous gp91 phox in PLB985 neutrophil-like cells overexpressing lentivirus (LV) EROS-GFP vector and treated with 10μg/mL cycloheximide. (I-J) gp91 phox expression following lentiviral transduction of EROS-GFP, gp91 phox or both in differentiated PL985 knock-out for p22 phox (I) or EROS (J) . n: representative of at least 3 independent experiments. See also Figure S1.
    Human P22 Phox Cyba, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti p22 phox antibody  (Santa Cruz Biotechnology)
    95
    Santa Cruz Biotechnology anti p22 phox antibody
    (A-C) Mouse constructs encoding EROS and gp91 <t>phox</t> were co-transfected into NIH3T3 (A) , COS-7 (B) and HEK293T (C) cell lines. gp91 phox expression was analysed by immunoblotting; arrow indicates gp91 phox band; ns: non-specific band. (D-F) gp91 phox and <t>p22</t> phox expression in HEK293T cells following transfection with the indicated human constructs. (G) Analysis of the stability of the different forms of gp91 phox (indicated by the arrows) following transfection in HEK293T cells in presence or absence of EROS and treatment with 10μg/mL cycloheximide. Actin and vinculin were used as loading control. (H) Stability of endogenous gp91 phox in PLB985 neutrophil-like cells overexpressing lentivirus (LV) EROS-GFP vector and treated with 10μg/mL cycloheximide. (I-J) gp91 phox expression following lentiviral transduction of EROS-GFP, gp91 phox or both in differentiated PL985 knock-out for p22 phox (I) or EROS (J) . n: representative of at least 3 independent experiments. See also Figure S1.
    Anti P22 Phox Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p22 phox antisera  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology p22 phox antisera
    (A-C) Mouse constructs encoding EROS and gp91 <t>phox</t> were co-transfected into NIH3T3 (A) , COS-7 (B) and HEK293T (C) cell lines. gp91 phox expression was analysed by immunoblotting; arrow indicates gp91 phox band; ns: non-specific band. (D-F) gp91 phox and <t>p22</t> phox expression in HEK293T cells following transfection with the indicated human constructs. (G) Analysis of the stability of the different forms of gp91 phox (indicated by the arrows) following transfection in HEK293T cells in presence or absence of EROS and treatment with 10μg/mL cycloheximide. Actin and vinculin were used as loading control. (H) Stability of endogenous gp91 phox in PLB985 neutrophil-like cells overexpressing lentivirus (LV) EROS-GFP vector and treated with 10μg/mL cycloheximide. (I-J) gp91 phox expression following lentiviral transduction of EROS-GFP, gp91 phox or both in differentiated PL985 knock-out for p22 phox (I) or EROS (J) . n: representative of at least 3 independent experiments. See also Figure S1.
    P22 Phox Antisera, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p22 phox shrna lentiviral particles  (Santa Cruz Biotechnology)
    85
    Santa Cruz Biotechnology p22 phox shrna lentiviral particles
    (A-C) Mouse constructs encoding EROS and gp91 <t>phox</t> were co-transfected into NIH3T3 (A) , COS-7 (B) and HEK293T (C) cell lines. gp91 phox expression was analysed by immunoblotting; arrow indicates gp91 phox band; ns: non-specific band. (D-F) gp91 phox and <t>p22</t> phox expression in HEK293T cells following transfection with the indicated human constructs. (G) Analysis of the stability of the different forms of gp91 phox (indicated by the arrows) following transfection in HEK293T cells in presence or absence of EROS and treatment with 10μg/mL cycloheximide. Actin and vinculin were used as loading control. (H) Stability of endogenous gp91 phox in PLB985 neutrophil-like cells overexpressing lentivirus (LV) EROS-GFP vector and treated with 10μg/mL cycloheximide. (I-J) gp91 phox expression following lentiviral transduction of EROS-GFP, gp91 phox or both in differentiated PL985 knock-out for p22 phox (I) or EROS (J) . n: representative of at least 3 independent experiments. See also Figure S1.
    P22 Phox Shrna Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p22 phox sirna  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology p22 phox sirna
    (A-C) Mouse constructs encoding EROS and gp91 <t>phox</t> were co-transfected into NIH3T3 (A) , COS-7 (B) and HEK293T (C) cell lines. gp91 phox expression was analysed by immunoblotting; arrow indicates gp91 phox band; ns: non-specific band. (D-F) gp91 phox and <t>p22</t> phox expression in HEK293T cells following transfection with the indicated human constructs. (G) Analysis of the stability of the different forms of gp91 phox (indicated by the arrows) following transfection in HEK293T cells in presence or absence of EROS and treatment with 10μg/mL cycloheximide. Actin and vinculin were used as loading control. (H) Stability of endogenous gp91 phox in PLB985 neutrophil-like cells overexpressing lentivirus (LV) EROS-GFP vector and treated with 10μg/mL cycloheximide. (I-J) gp91 phox expression following lentiviral transduction of EROS-GFP, gp91 phox or both in differentiated PL985 knock-out for p22 phox (I) or EROS (J) . n: representative of at least 3 independent experiments. See also Figure S1.
    P22 Phox Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A-C) Mouse constructs encoding EROS and gp91 phox were co-transfected into NIH3T3 (A) , COS-7 (B) and HEK293T (C) cell lines. gp91 phox expression was analysed by immunoblotting; arrow indicates gp91 phox band; ns: non-specific band. (D-F) gp91 phox and p22 phox expression in HEK293T cells following transfection with the indicated human constructs. (G) Analysis of the stability of the different forms of gp91 phox (indicated by the arrows) following transfection in HEK293T cells in presence or absence of EROS and treatment with 10μg/mL cycloheximide. Actin and vinculin were used as loading control. (H) Stability of endogenous gp91 phox in PLB985 neutrophil-like cells overexpressing lentivirus (LV) EROS-GFP vector and treated with 10μg/mL cycloheximide. (I-J) gp91 phox expression following lentiviral transduction of EROS-GFP, gp91 phox or both in differentiated PL985 knock-out for p22 phox (I) or EROS (J) . n: representative of at least 3 independent experiments. See also Figure S1.

    Journal: bioRxiv

    Article Title: EROS-mediated control of NOX2 and P2X7 biosynthesis

    doi: 10.1101/2021.09.14.460103

    Figure Lengend Snippet: (A-C) Mouse constructs encoding EROS and gp91 phox were co-transfected into NIH3T3 (A) , COS-7 (B) and HEK293T (C) cell lines. gp91 phox expression was analysed by immunoblotting; arrow indicates gp91 phox band; ns: non-specific band. (D-F) gp91 phox and p22 phox expression in HEK293T cells following transfection with the indicated human constructs. (G) Analysis of the stability of the different forms of gp91 phox (indicated by the arrows) following transfection in HEK293T cells in presence or absence of EROS and treatment with 10μg/mL cycloheximide. Actin and vinculin were used as loading control. (H) Stability of endogenous gp91 phox in PLB985 neutrophil-like cells overexpressing lentivirus (LV) EROS-GFP vector and treated with 10μg/mL cycloheximide. (I-J) gp91 phox expression following lentiviral transduction of EROS-GFP, gp91 phox or both in differentiated PL985 knock-out for p22 phox (I) or EROS (J) . n: representative of at least 3 independent experiments. See also Figure S1.

    Article Snippet: The following plasmids were obtained from Origene: mouse gp91 phox /nox2/cybb (MC204867), mouse GFP-tagged gp91 phox /nox2/cybb (MG208975), mouse EROS/cybc1 (MC201263), human EROS/CYBC1 (SC324452), human gp91 phox /CYBB (SC122091), human p22 phox /CYBA (SC101113), human NOX4 (SC322623), mouse GFP-tagged P2X7 (MR227216), human P2X1 (SC118594) and human P2X4 (SC122124).

    Techniques: Construct, Transfection, Expressing, Western Blot, Plasmid Preparation, Transduction, Knock-Out

    (A-D) Immunoprecipitation (IP) and size exclusion chromatography (SEC) analysis of protein complexes associated with EROS. (A) IP of EROS in HEK293-F cells expressing StrepII-FLAG-tagged EROS, gp91 phox -GFP and p22 phox with western blot for gp91 phox . Lysates treated with Peptide N-glycosidase F (PNGaseF) or Endoglycosidase H (EndoH) served as reference; PG: partially glycosylated; NG: non-glycosylated; RT: run through (B) SEC profile of EROS-IP eluate indicating protein (280nm) and heme (414nm) content. (C) Immunoblot analysis of gp91 phox -GFP, EROS-FLAG and endogenous p22 phox in SEC fraction 9-14 and 15-18. (D) SEC profile of EROS eluate from HEK293-F cells expressing EROS-FLAG, gp91 phox and p22 phox constructs and treated with heme biosynthesis inhibitor succinyl acetone (10µg/ml). (E) IP of StrepII-FLAG-tagged EROS in HEK293-F treated with succinyl acetone. (F) Interaction between gp91 phox and EROS assessed through luminescence production in live HEK293T cells expressing the indicated plasmids fused with the large (LgBIT) or small (SmBIT) fragment of the NanoLuc luciferase (see methods). Halo Tag (HT)-SmBIT is the negative control; RLU: Relative Luminescence Unit. ( G) Yeast growth phenotypes obtained with the specified selective media using gp91 phox bait plasmid and EROS prey plasmid. DBD: DNA binding domain of Gal4; AD: Activation domain of Gal4 (see methods). (H) EROS localisation in HEK293 cells transfected with EROS construct (top panel; 3D stack) or EROS and Lap2-GFP constructs (bottom panel; single plane), fixed, permeabilised and labelled with anti-EROS and anti-calnexin antibodies. Scale bar= 5μm. n= representative of at least 3 independent experiments. See also Figure S2.

    Journal: bioRxiv

    Article Title: EROS-mediated control of NOX2 and P2X7 biosynthesis

    doi: 10.1101/2021.09.14.460103

    Figure Lengend Snippet: (A-D) Immunoprecipitation (IP) and size exclusion chromatography (SEC) analysis of protein complexes associated with EROS. (A) IP of EROS in HEK293-F cells expressing StrepII-FLAG-tagged EROS, gp91 phox -GFP and p22 phox with western blot for gp91 phox . Lysates treated with Peptide N-glycosidase F (PNGaseF) or Endoglycosidase H (EndoH) served as reference; PG: partially glycosylated; NG: non-glycosylated; RT: run through (B) SEC profile of EROS-IP eluate indicating protein (280nm) and heme (414nm) content. (C) Immunoblot analysis of gp91 phox -GFP, EROS-FLAG and endogenous p22 phox in SEC fraction 9-14 and 15-18. (D) SEC profile of EROS eluate from HEK293-F cells expressing EROS-FLAG, gp91 phox and p22 phox constructs and treated with heme biosynthesis inhibitor succinyl acetone (10µg/ml). (E) IP of StrepII-FLAG-tagged EROS in HEK293-F treated with succinyl acetone. (F) Interaction between gp91 phox and EROS assessed through luminescence production in live HEK293T cells expressing the indicated plasmids fused with the large (LgBIT) or small (SmBIT) fragment of the NanoLuc luciferase (see methods). Halo Tag (HT)-SmBIT is the negative control; RLU: Relative Luminescence Unit. ( G) Yeast growth phenotypes obtained with the specified selective media using gp91 phox bait plasmid and EROS prey plasmid. DBD: DNA binding domain of Gal4; AD: Activation domain of Gal4 (see methods). (H) EROS localisation in HEK293 cells transfected with EROS construct (top panel; 3D stack) or EROS and Lap2-GFP constructs (bottom panel; single plane), fixed, permeabilised and labelled with anti-EROS and anti-calnexin antibodies. Scale bar= 5μm. n= representative of at least 3 independent experiments. See also Figure S2.

    Article Snippet: The following plasmids were obtained from Origene: mouse gp91 phox /nox2/cybb (MC204867), mouse GFP-tagged gp91 phox /nox2/cybb (MG208975), mouse EROS/cybc1 (MC201263), human EROS/CYBC1 (SC324452), human gp91 phox /CYBB (SC122091), human p22 phox /CYBA (SC101113), human NOX4 (SC322623), mouse GFP-tagged P2X7 (MR227216), human P2X1 (SC118594) and human P2X4 (SC122124).

    Techniques: Immunoprecipitation, Size-exclusion Chromatography, Expressing, Western Blot, Construct, Luciferase, Negative Control, Plasmid Preparation, Binding Assay, Activation Assay, Transfection