Journal: Infection and Immunity
Article Title: Lipopolysaccharide Preparation Extracted from Porphyromonas gingivalis Lipoprotein-Deficient Mutant Shows a Marked Decrease in Toll-Like Receptor 2-Mediated Signaling
Figure Lengend Snippet: Inactivation of PG1828 in P. gingivalis . (A) Construction of a PG1828-deficient mutant of P. gingivalis strain 381 by allelic exchange mutagenesis with an ermF-ermAM antibiotic resistance cassette (as described in Materials and Methods). (B) Predicted maps of the genomes of the wild-type and the PG1828-deficient mutant. Arrowheads indicate the numbers and positions of oligonucleotide primers for PCR analysis (as described in Materials and Methods). (C) PCR analysis of WT and ΔPG1828. Numbers above the lanes indicate the primer pairs (B) used for the PCR analysis. Lane M, DNA marker; left margin, molecular sizes.
Article Snippet: P. gingivalis strain 381 was grown anaerobically at 37°C in brain heart infusion (BHI) broth (Difco, Detroit, Mich.) containing 0.5% yeast extract (Difco), 5 μg of hemin per ml, and 1 μg of vitamin K3 per ml.
Techniques: Mutagenesis, Polymerase Chain Reaction, Marker