Journal: Scientific Reports
Article Title: A two-component system regulates gene expression of the type IX secretion component proteins via an ECF sigma factor
Figure Lengend Snippet: Structure and localization of PorX and PorY. ( A ) Schematic representation of the functional domains of PorX and PorY. The symbols denote the following, RR, response regulatory domain; PglZ, PglZ motif; ALP, alkaline phosphatase-like core domain; SS, signal sequence; TM, transmembrane domain; HK1, histidine kinase phosphoacceptor domain; HK2, histidine kinase ATPase domain. Arrows below the schema indicate the recombinant proteins generated in this study. The domains were predicted by the Kyoto Encyclopedia of Genes and Genomes (KEGG) Sequence Similarity DataBase (KEGG SSDB). ( B ) Subcellular localization of PorX and PorY in P. gingivalis. Fractionated cell lysates of the wild-type strain ATCC 33277 were subjected to immunodetection with antisera against PorX and PorY. WC, whole cell lysate; C/P, cytoplasm/periplasm; CE, cell envelope; IM, inner membrane; OM, outer membrane. Arrowheads indicate the immunoreacting PorX and PorY bands. ( C ) SDS-PAGE profile of the purified recombinant proteins.
Article Snippet: Probes used in this assay were designed from the possible transcription starting sites in the tiling microarray, and PCR-amplified from P. gingivalis ATCC 33277 DNA using primers shown in .
Techniques: Functional Assay, ALP Assay, Sequencing, Recombinant, Generated, Immunodetection, SDS Page, Purification