Journal: PLoS ONE
Article Title: Antigenicity and Immunogenicity of Plasmodium vivax Merozoite Surface Protein-3
Figure Lengend Snippet: Immunofluorescence of P. vivax parasites and anti-MSP-3 antibodies. Immunofluorescence patterns in the sera of mice immunized with recombinant PvMSP-3α and PvMSP-3β (FP-3) on acetone-fixed P. vivax -infected erythrocytes (Pv-iE). The smears were incubated with pooled antisera (1∶100) from mice immunized with: ( A ) PBS emulsified in adjuvant, ( B ) PvMSP-3α, or ( C ) PvMSP-3β in Freund’s Adjuvant. Antibody binding was detected with secondary Alexa 568-labeled antibody (red) and nuclei were visualized by DAPI staining (blue). BF, bright field.
Article Snippet: Pooled sera from mice immunized 3 times with the recombinant PvMSP-3α and PvMSP-3β (FP-3) proteins emulsified in Freund’s Adjuvant (1∶100) were applied to the smear and incubated for 1 h before incubation with secondary anti-mouse IgG antibody conjugated to Alexa Fluor 568 (Invitrogen) or DAPI (4′,6-diamidino-2-phenylindole, dihydrochloride, Invitrogen).
Techniques: Immunofluorescence, Mouse Assay, Recombinant, Infection, Incubation, Binding Assay, Labeling, Staining