Journal: BMC Microbiology

Article Title: Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane

doi: 10.1186/s12866-018-1381-7

Figure Lengend Snippet: Release of intracellular K + in the presence of paenipeptin C′ as detected using a potassium-sensitive fluorescent probe, PBFI. a Pseudomonas aeruginosa ATCC 27853 ( b ) Staphylococcus aureus ATCC 29213. Means with different letters are significantly different between groups ( p

Article Snippet: As shown in Fig. a, substantial morphology changes occurred when the P. aeruginosa ATCC 27853 cells were treated with paenipeptin C′ at 16 μg/mL for 2 h. Conversely, the non-treated P. aeruginosa cells displayed the typical Gram-negative rod-shaped structures with intact membrane and high density cytoplasm (Fig. b).

Techniques:

Journal: BMC Microbiology

Article Title: Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane

doi: 10.1186/s12866-018-1381-7

Figure Lengend Snippet: Examination of the cell morphology change after paenipeptin C′ treatment using transmission electron microscopy (TEM). a Pseudomonas aeruginosa ATCC 27853 cells were treated by paenipeptin C′ at 16 μg/mL for 2 h. b Non-treated P. aeruginosa ATCC 27853 cells. c Staphylococcus aureus ATCC 29213 cells were treated by paenipeptin C' at 32 μg/mL for 2 h. d Non-treated S. aureus ATCC 29213 cells

Article Snippet: As shown in Fig. a, substantial morphology changes occurred when the P. aeruginosa ATCC 27853 cells were treated with paenipeptin C′ at 16 μg/mL for 2 h. Conversely, the non-treated P. aeruginosa cells displayed the typical Gram-negative rod-shaped structures with intact membrane and high density cytoplasm (Fig. b).

Techniques: Transmission Assay, Electron Microscopy, Transmission Electron Microscopy

Journal: BMC Microbiology

Article Title: Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane

doi: 10.1186/s12866-018-1381-7

Figure Lengend Snippet: Concentration-dependent bactericidal activity of lipopeptide paenipeptin C′ against ( a ) Pseudomonas aeruginosa ATCC 27853 and ( b ) Staphylococcus aureus ATCC 29213

Article Snippet: As shown in Fig. a, substantial morphology changes occurred when the P. aeruginosa ATCC 27853 cells were treated with paenipeptin C′ at 16 μg/mL for 2 h. Conversely, the non-treated P. aeruginosa cells displayed the typical Gram-negative rod-shaped structures with intact membrane and high density cytoplasm (Fig. b).

Techniques: Concentration Assay, Activity Assay

Journal: BMC Microbiology

Article Title: Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane

doi: 10.1186/s12866-018-1381-7

Figure Lengend Snippet: Changes in bacterial membrane potential in the presence of paenipeptin C′ as determined using a membrane potential-sensitive fluorescent probe, DiSC 3 (5). a Pseudomonas aeruginosa ATCC 27853 ( b ) Staphylococcus aureus ATCC 29213. Means with different letters are significantly different between groups ( p

Article Snippet: As shown in Fig. a, substantial morphology changes occurred when the P. aeruginosa ATCC 27853 cells were treated with paenipeptin C′ at 16 μg/mL for 2 h. Conversely, the non-treated P. aeruginosa cells displayed the typical Gram-negative rod-shaped structures with intact membrane and high density cytoplasm (Fig. b).

Techniques:

Journal: BMC Microbiology

Article Title: Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane

doi: 10.1186/s12866-018-1381-7

Figure Lengend Snippet: Impact of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) on antimicrobial activity of paenipeptin C′ against ( a ) Pseudomonas aeruginosa ATCC 27853 and ( b ) Staphylococcus aureus ATCC 29213. The nontreated cells (Non) without antibiotics and LPS/LTA were used as control groups. Means with different letters are significantly different between groups ( p

Article Snippet: As shown in Fig. a, substantial morphology changes occurred when the P. aeruginosa ATCC 27853 cells were treated with paenipeptin C′ at 16 μg/mL for 2 h. Conversely, the non-treated P. aeruginosa cells displayed the typical Gram-negative rod-shaped structures with intact membrane and high density cytoplasm (Fig. b).

Techniques: Activity Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: New Amphiphilic Neamine Derivatives Active against Resistant Pseudomonas aeruginosa and Their Interactions with Lipopolysaccharides

doi: 10.1128/AAC.02536-13

Figure Lengend Snippet: Bacterial killing of P. aeruginosa ATCC 27853 by 3′,6-diNn at different concentrations expressed as the multiple of the MIC. The ordinate shows the change in the number of CFU (log scale) per milliliter. The solid horizontal line corresponds to

Article Snippet: The effect of 3′,6-diNn on the formation of a P. aeruginosa ATCC 27853 biofilm was determined by quantifying the biomass using the crystal violet staining method , while the viability of bacterial cells was assessed by monitoring the intracellular hydrolysis of fluorescein diacetate ( ).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: New Amphiphilic Neamine Derivatives Active against Resistant Pseudomonas aeruginosa and Their Interactions with Lipopolysaccharides

doi: 10.1128/AAC.02536-13

Figure Lengend Snippet: Effect of 3′,6-dialkyl neamine derivatives on the OM permeability in living P. aeruginosa ATCC 27853 cells assessed by the increase in the fluorescence of NPN. (A) Dose-dependent NPN uptake within the bacterial OM expressed as a percentage of

Article Snippet: The effect of 3′,6-diNn on the formation of a P. aeruginosa ATCC 27853 biofilm was determined by quantifying the biomass using the crystal violet staining method , while the viability of bacterial cells was assessed by monitoring the intracellular hydrolysis of fluorescein diacetate ( ).

Techniques: Permeability, Fluorescence

Journal: Antimicrobial Agents and Chemotherapy

Article Title: New Amphiphilic Neamine Derivatives Active against Resistant Pseudomonas aeruginosa and Their Interactions with Lipopolysaccharides

doi: 10.1128/AAC.02536-13

Figure Lengend Snippet: Inhibition of biofilm formation of P. aeruginosa ATCC 27853 incubated with 3′,6-diNn (A) or gentamicin (B) at different concentrations expressed as a multiple of their corresponding MICs. biofilm mass (closed bars), assessed by the crystal violet

Article Snippet: The effect of 3′,6-diNn on the formation of a P. aeruginosa ATCC 27853 biofilm was determined by quantifying the biomass using the crystal violet staining method , while the viability of bacterial cells was assessed by monitoring the intracellular hydrolysis of fluorescein diacetate ( ).

Techniques: Inhibition, Incubation

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Antimicrobial Activity of Triphala Constituents and Nanoformulation

doi: 10.1155/2020/6976973

Figure Lengend Snippet: Microdilution broth assay of TRIT 4–TRIT 8 against P. aeruginosa ATCC 27853.

Article Snippet: The resulting filters were then placed on a Mueller Hinton agar (MHA) plate on which a bacterial suspension (OD600 nm = 0.5) of the quality control strain P. aeruginosa ATCC 27853 was spread.

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Antimicrobial Activity of Triphala Constituents and Nanoformulation

doi: 10.1155/2020/6976973

Figure Lengend Snippet: Disc diffusion test of TRIT 1–TRIT 8 against P. aeruginosa ATCC 27853; TRIT 9 was the total Triphala hydroalcoholic extract.

Article Snippet: The resulting filters were then placed on a Mueller Hinton agar (MHA) plate on which a bacterial suspension (OD600 nm = 0.5) of the quality control strain P. aeruginosa ATCC 27853 was spread.

Techniques: Diffusion-based Assay

Journal: Journal of Food Science and Technology

Article Title: Characterization of biofilm production by Pseudomonas fluorescens isolated from refrigerated raw buffalo milk

doi: 10.1007/s13197-019-03924-1

Figure Lengend Snippet: Fluorescence in culture medium containing calcofluor white at 365 nm. S. epidermidis ATCC 35984, P. fluorescens NCTC 10038, P. aeruginosa ATCC 27853, PL5.4 ( P. fluorescens PL5.4), PL7.1 ( P. fluorescens PL7.1)

Article Snippet: Investigation of the production of exopolysaccharides (EPS) by solid medium containing calcofluor white In the evaluation of EPS production, the P. fluorescens PL5.4, P. fluorescens NCTC 10038 and P. aeruginosa ATCC 27853 bacteria showed fluorescence when exposed to UV light at a wavelength of 365 nm.

Techniques: Fluorescence

Journal: Scientific Reports

Article Title: Mechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa

doi: 10.1038/s41598-018-19434-7

Figure Lengend Snippet: LPS binding assay. Affinities of peptides toward LPS were examined by determining the effect of increasing LPS concentrations on their antimicrobial activities at ( a ) 1× MIC, and ( b ) 2× MIC, against P. aeruginosa . ( c ) CD spectra of peptides in 0.1% LPS suspensions. The peptide concentration was fixed at 40 µM. Each experiment was repeated three times.

Article Snippet: LPS binding assay To study the effect generated by interactions between lipopolysaccharides (LPS) and peptides, we monitored the antimicrobial activity against P. aeruginosa ATCC 27853.

Techniques: Binding Assay, Concentration Assay

Journal: Scientific Reports

Article Title: Mechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa

doi: 10.1038/s41598-018-19434-7

Figure Lengend Snippet: Fluorescence microscopy images of live/dead stained P. aeruginosa biofilms after treatment with peptides at 0.5× or 1× MBIC. Living cells are stained in green (SYTO9 dye), while dead cells are stained in red (propidium iodide). ( a ) P. aeruginosa ATCC 27853 ( b ) P. aeruginosa 1034. Scale bar = 50 µM.

Article Snippet: LPS binding assay To study the effect generated by interactions between lipopolysaccharides (LPS) and peptides, we monitored the antimicrobial activity against P. aeruginosa ATCC 27853.

Techniques: Fluorescence, Microscopy, Staining