Journal: PLoS ONE

Article Title: Discovery of New Molecular Subtypes in Oesophageal Adenocarcinoma

doi: 10.1371/journal.pone.0023985

Figure Lengend Snippet: Multivariate Cox regression analyses for p-HSP27 (Ser15) , HER2, pT, pN and cM.

Article Snippet: p-HER2 (Tyr1248) , #44-900 , Invitrogen , 1∶1000.

Techniques:

Journal: PLoS ONE

Article Title: Discovery of New Molecular Subtypes in Oesophageal Adenocarcinoma

doi: 10.1371/journal.pone.0023985

Figure Lengend Snippet: The 87 oesophageal adenocarcinoma cases were clustered according to the expression of the investigated members of the (p)HSP27 group and the HER family. Two tumour clusters could be found. Cluster 1 was composed of mainly low HER2-family and high (p)HSP27-group expressing cases, whereas Cluster 2 showed a reverse pattern. Cluster colour key: Red – up-regulated; green – down-regulated; black – unchanged; grey – missing.

Article Snippet: p-HER2 (Tyr1248) , #44-900 , Invitrogen , 1∶1000.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Discovery of New Molecular Subtypes in Oesophageal Adenocarcinoma

doi: 10.1371/journal.pone.0023985

Figure Lengend Snippet: Cluster 1, which mainly consisted of low HER2 family and high (p)HSP27 group-expressing cases was comprised of few pN (21%) and no cM (0%) positive cases, whereas Cluster 2 (high HER2 family and low (p)HSP27 group) was comprised of significantly more pN- and cM-positive cases (67% and 15%, respectively). Consistent with these data, patients in Cluster 1 had significantly better overall survival times than Cluster 2.

Article Snippet: p-HER2 (Tyr1248) , #44-900 , Invitrogen , 1∶1000.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Discovery of New Molecular Subtypes in Oesophageal Adenocarcinoma

doi: 10.1371/journal.pone.0023985

Figure Lengend Snippet: Proteins analysed and antibodies used in this study.

Article Snippet: p-HER2 (Tyr1248) , #44-900 , Invitrogen , 1∶1000.

Techniques:

Journal: Cell Adhesion & Migration

Article Title: Cathelicidin LL-37 promotes EMT, migration and metastasis of hepatocellular carcinoma cells in vitro and mouse model

doi: 10.1080/19336918.2023.2168231

Figure Lengend Snippet: LL-37 promoted EMT via HER2/EGFR-MAPK/ERK signaling in HCC cells. PLC/PRF-5 and Huh7 cells were transfected with pcDNA/LL-37 (a), or si-LL-37 (b), and the MMP14, MMP9 and p-ERK1/2 level was detected by western blot. PLC/PRF-5 and Huh7 cells were transfected with pcDNA/LL-37 for 48 h, then KO-947 (c) or Neratinib (d) was added. Western blots detected the levels of p-ERK1/2, ERK1/2, Vimentin, E-cadherin, N-cadherin, Slug and Snail. Transwell assay (e) and wound healing assay (f) were conducted to evaluate changes in invasion and migration after KO-947 or neratinib treatment, respectively. Multiple of invasion cells and the wound closure (%) compared with control (pcDNA3.0) were calculated from 4 independent experiments. Data are represented as the mean ± SEM. **p < 0.01, ***p < 0.001.

Article Snippet: Antibodies against β-actin and p-HER2 (Tyr1248) were purchased from Abclonal (Wuhan, China), as well as anti-rabbit and anti-mouse secondary antibodies.

Techniques: Transfection, Western Blot, Transwell Assay, Wound Healing Assay, Migration

Journal: Cell Adhesion & Migration

Article Title: Cathelicidin LL-37 promotes EMT, migration and metastasis of hepatocellular carcinoma cells in vitro and mouse model

doi: 10.1080/19336918.2023.2168231

Figure Lengend Snippet: The effect of LL-37 expression on EMT in xenograft tumors. PLC/PRF-5 and PLC/PRF-5 LL−37 (stable overexpressing LL-37 cells) were subcutaneously injected into nude mice to establish an xenograft mouse model, and the mice were assigned to three groups: PLC/PRF-5 xenografted mouse group (control group), PLC/PRF-5 LL−37 xenograft mouse group (OV-LL-37 group) and PLC/PRF-5 xenografted mouse group with si-LL-37 treatment group (si-LL-37 group). Mice were euthanatized on day 28. (a) Tumor tissues were crushed, lysed with RIPA lysis buffer and supernatants were collected to detect p-HER2, p-EGFR, p-ERK1/2 and EMT markers by western blot. The quantified data was normalized to the protein expression of control group cells (n = 4). (b) Total RNA from tumor tissues was extracted and the expression of EMT markers was detected by qRT-PCR. (c) Tumor tissues were embedded in paraffin and sectioned for IHC staining using antibodies against EMT markers. Representative images from each group are shown. Scale bars, 50 μm. Data are represented as means ± SEM. ns, no significance. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Antibodies against β-actin and p-HER2 (Tyr1248) were purchased from Abclonal (Wuhan, China), as well as anti-rabbit and anti-mouse secondary antibodies.

Techniques: Expressing, Injection, Lysis, Western Blot, Quantitative RT-PCR, Immunohistochemistry

Journal: Cell Adhesion & Migration

Article Title: Cathelicidin LL-37 promotes EMT, migration and metastasis of hepatocellular carcinoma cells in vitro and mouse model

doi: 10.1080/19336918.2023.2168231

Figure Lengend Snippet: LL-37 silencing enhanced 1,25(OH) 2 D 3 -induced EMT in vivo. (a) PLC/PRF-5 xenograft tumors were crushed and lysed with RIPA lysis buffer, and then western blot detection was performed using the indicated antibodies. (b) Representative images of IHC of E-cadherin, N-cadherin and Slug in xenograft tumors. Scale bar: 50 μm. Data are represented as means ± SEM. (c) A schematic diagram summarizing the regulation mechanism of LL-37 on the EMT, migration and metastasis of HCC cells. 1,25(OH) 2 D 3 induces the secretory expression of LL-37 through VDR receptor signal. Then LL-37 phosphorylates EGFR/HER2 to stimulate MAPK/ERK signaling, regulate the expression of EMT-related transcription factors or markers. Ultimately affects the EMT, migration and metastasis of HCC cells. ns, no significance. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Antibodies against β-actin and p-HER2 (Tyr1248) were purchased from Abclonal (Wuhan, China), as well as anti-rabbit and anti-mouse secondary antibodies.

Techniques: In Vivo, Lysis, Western Blot, Migration, Expressing