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  • 99
    ATCC p gingivalis p gingivalis atcc 33277
    Arginine specific amidolytic activity (Arg act) measured by Δ abs 405 nm after addition of taurolidine (TAU; a , c , e , g ) and minocycline (MINO; b , d , f , h ) in different concentrations to 10 nM RgpB ( a , b ), Porphyromonas <t>gingivalis</t> <t>ATCC</t> 33277 ( c , d ), P. gingivalis J374-1 ( e , f ) and P. gingivalis HG66 ( g , h ).
    P Gingivalis P Gingivalis Atcc 33277, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    ATCC p gingivalis atcc
    Endotoxin activity (endotoxin units (EU), mean ± SD) measured by Limulus amebocyte lysate assay of Escherichia coli (E.c.) LPS, Porphyromonas <t>gingivalis</t> (P.g.) LPS as well as P. gingivalis <t>ATCC</t> 33,277 and Aggregatibacter actinomycetemcomitans Y4, without and with 2 h pre-exposure to 0.01%, 0.25%, 1% taurolidine (TAU) and 16 µg/mL minocycline (MINO).* p
    P Gingivalis Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    vazyme biotech co p gingivalis atcc 33277
    Endotoxin activity (endotoxin units (EU), mean ± SD) measured by Limulus amebocyte lysate assay of Escherichia coli (E.c.) LPS, Porphyromonas <t>gingivalis</t> (P.g.) LPS as well as P. gingivalis <t>ATCC</t> 33,277 and Aggregatibacter actinomycetemcomitans Y4, without and with 2 h pre-exposure to 0.01%, 0.25%, 1% taurolidine (TAU) and 16 µg/mL minocycline (MINO).* p
    P Gingivalis Atcc 33277, supplied by vazyme biotech co, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p gingivalis atcc 33277/product/vazyme biotech co
    Average 91 stars, based on 1 article reviews
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    88
    ATCC p gingivalis atcc 53977
    Endotoxin activity (endotoxin units (EU), mean ± SD) measured by Limulus amebocyte lysate assay of Escherichia coli (E.c.) LPS, Porphyromonas <t>gingivalis</t> (P.g.) LPS as well as P. gingivalis <t>ATCC</t> 33,277 and Aggregatibacter actinomycetemcomitans Y4, without and with 2 h pre-exposure to 0.01%, 0.25%, 1% taurolidine (TAU) and 16 µg/mL minocycline (MINO).* p
    P Gingivalis Atcc 53977, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC p gingivalis atcc 49417
    Endotoxin activity (endotoxin units (EU), mean ± SD) measured by Limulus amebocyte lysate assay of Escherichia coli (E.c.) LPS, Porphyromonas <t>gingivalis</t> (P.g.) LPS as well as P. gingivalis <t>ATCC</t> 33,277 and Aggregatibacter actinomycetemcomitans Y4, without and with 2 h pre-exposure to 0.01%, 0.25%, 1% taurolidine (TAU) and 16 µg/mL minocycline (MINO).* p
    P Gingivalis Atcc 49417, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC p gingivalis atcc 53978
    Distribution of PAD in various strains of P. <t>gingivalis</t> . The production of citrulline from BAEE was determined in cellular (open bar), supernatant (solid bar), and vesicle (hatched bar) fractions from HG66, <t>ATCC</t> 53978 (W50), and ATCC 33277 strains of P. gingivalis .
    P Gingivalis Atcc 53978, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC p gingivalis atcc 32277
    Distribution of PAD in various strains of P. <t>gingivalis</t> . The production of citrulline from BAEE was determined in cellular (open bar), supernatant (solid bar), and vesicle (hatched bar) fractions from HG66, <t>ATCC</t> 53978 (W50), and ATCC 33277 strains of P. gingivalis .
    P Gingivalis Atcc 32277, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Arginine specific amidolytic activity (Arg act) measured by Δ abs 405 nm after addition of taurolidine (TAU; a , c , e , g ) and minocycline (MINO; b , d , f , h ) in different concentrations to 10 nM RgpB ( a , b ), Porphyromonas gingivalis ATCC 33277 ( c , d ), P. gingivalis J374-1 ( e , f ) and P. gingivalis HG66 ( g , h ).

    Journal: Antibiotics

    Article Title: Taurolidine Acts on Bacterial Virulence Factors and Does Not Induce Resistance in Periodontitis-Associated Bacteria—An In-Vitro Study

    doi: 10.3390/antibiotics9040166

    Figure Lengend Snippet: Arginine specific amidolytic activity (Arg act) measured by Δ abs 405 nm after addition of taurolidine (TAU; a , c , e , g ) and minocycline (MINO; b , d , f , h ) in different concentrations to 10 nM RgpB ( a , b ), Porphyromonas gingivalis ATCC 33277 ( c , d ), P. gingivalis J374-1 ( e , f ) and P. gingivalis HG66 ( g , h ).

    Article Snippet: Applying 1% taurolidine to P. gingivalis ATCC 33277 and J374-1, the measured arginine-specific amidolytic activity clearly decreased, whereas 0.01% did not exert any effect on the enzyme activity.

    Techniques: Activity Assay

    Transmission electron micrographs (TEM) images of Porphyromonas gingivalis ATCC 33277 and Tannerella forsythia Be13237 after treatment with 1% taurolidine for 2 h, in comparison to its control. P. gingivalis shows a decomposition of the cytoplasm leading to unstained areas (arrowheads). For T. forsythia , no difference could be found. Scale bars: 200 nm.

    Journal: Antibiotics

    Article Title: Taurolidine Acts on Bacterial Virulence Factors and Does Not Induce Resistance in Periodontitis-Associated Bacteria—An In-Vitro Study

    doi: 10.3390/antibiotics9040166

    Figure Lengend Snippet: Transmission electron micrographs (TEM) images of Porphyromonas gingivalis ATCC 33277 and Tannerella forsythia Be13237 after treatment with 1% taurolidine for 2 h, in comparison to its control. P. gingivalis shows a decomposition of the cytoplasm leading to unstained areas (arrowheads). For T. forsythia , no difference could be found. Scale bars: 200 nm.

    Article Snippet: Applying 1% taurolidine to P. gingivalis ATCC 33277 and J374-1, the measured arginine-specific amidolytic activity clearly decreased, whereas 0.01% did not exert any effect on the enzyme activity.

    Techniques: Transmission Assay, Transmission Electron Microscopy

    Binding of the human plasma proteins HPG-Bt and HK-Bt to C. albicans hyphae pretreated with enzymes secreted into the growth medium (m) by P. gingivalis (PG) strains: ( A ) ATCC 33277 and Δppad (ATCC 33277) and ( B ) W83 and Δppad (W83). C. albicans cells were incubated for 6 h in RPMI 1640 medium with the further addition of media collected after P. gingivalis culture. Fungal cells grown in RPMI 1640 medium with BHI or TSBY broth instead of culture supernatants from appropriate P. gingivalis strains served as a control. The bound biotinylated human protein was quantified using the SA-HRP/TMB detection system. Results from representative experiments are shown; bars represent the mean values ± SEM. Statistical analysis was performed by one-way ANOVA with a Tukey’s multiple comparisons test ( p

    Journal: International Journal of Molecular Sciences

    Article Title: Peptidylarginine Deiminase of Porphyromonas gingivalis Modulates the Interactions between Candida albicans Biofilm and Human Plasminogen and High-Molecular-Mass Kininogen

    doi: 10.3390/ijms21072495

    Figure Lengend Snippet: Binding of the human plasma proteins HPG-Bt and HK-Bt to C. albicans hyphae pretreated with enzymes secreted into the growth medium (m) by P. gingivalis (PG) strains: ( A ) ATCC 33277 and Δppad (ATCC 33277) and ( B ) W83 and Δppad (W83). C. albicans cells were incubated for 6 h in RPMI 1640 medium with the further addition of media collected after P. gingivalis culture. Fungal cells grown in RPMI 1640 medium with BHI or TSBY broth instead of culture supernatants from appropriate P. gingivalis strains served as a control. The bound biotinylated human protein was quantified using the SA-HRP/TMB detection system. Results from representative experiments are shown; bars represent the mean values ± SEM. Statistical analysis was performed by one-way ANOVA with a Tukey’s multiple comparisons test ( p

    Article Snippet: Growth of C. albicans Cells in Media Collected after Culture of P. gingivalis C. albicans cells were first aerobically cultured in 20 mL of YPD medium for 16 h at 30 °C with shaking (170 rpm), and a 20 μL aliquot of the cell suspension was transferred to 20 mL of medium collected after 24 h of growth of P. gingivalis strains ATCC 33277, Δppad (ATCC 33277), W83, Δppad (W83) or to 20 mL of BHI or TSBY media, and further incubated for 18 h at 37 °C with shaking (170 rpm) under normoxia or anoxia.

    Techniques: Binding Assay, Incubation

    The arrangement of porR and its neighbouring genes in P. gingivalis ATCC 33277 genome. porR (PGN_1236) and its neighbouring genes are flanked by IS5 family transposons that formed a composite transposon of 70 kbp in length. The genes that involve in biosynthesis of A-LPS are represented by yellow rectangles while the gene that does not involve is represented by brown rectangle. The genes for hypothetical proteins are represented by white rectangles. The genes for IS5 family transposases are represented by cyan rectangles. The purple triangles represented 12 bp inverted repeats that flanked the genes for IS5 family transposases. Name of proteins encoded by the genes are shown under rectangles that represented the genes. The slashes indicated gaps in the genome.

    Journal: PeerJ

    Article Title: Phylogenetic comparison between Type IX Secretion System (T9SS) protein components suggests evidence of horizontal gene transfer

    doi: 10.7717/peerj.9019

    Figure Lengend Snippet: The arrangement of porR and its neighbouring genes in P. gingivalis ATCC 33277 genome. porR (PGN_1236) and its neighbouring genes are flanked by IS5 family transposons that formed a composite transposon of 70 kbp in length. The genes that involve in biosynthesis of A-LPS are represented by yellow rectangles while the gene that does not involve is represented by brown rectangle. The genes for hypothetical proteins are represented by white rectangles. The genes for IS5 family transposases are represented by cyan rectangles. The purple triangles represented 12 bp inverted repeats that flanked the genes for IS5 family transposases. Name of proteins encoded by the genes are shown under rectangles that represented the genes. The slashes indicated gaps in the genome.

    Article Snippet: Identification of porR and its neighbouring genes’ arrangement in Porphyromonas gingivalis ATCC 33277 genome The sequence of P. gingivalis ATCC 33277 genome and annotation files of the genome were retrieved from Genbank ( ).

    Techniques:

    Gingipain adhesins in VDS fractions from P. gingivalis ATCC 33277 and rgp mutant strains. (A) VDS fractions from parent and mutant strains after SDS-PAGE and silver staining. (B) Western blot of the VDS fractions using a polyclonal antibody to gingipain adhesin domains. The 44-, 39-, 27-, and 19-kDa peptides in the fraction from ATCC 33277 are indicated by arrowheads. The 44-kDa peptide was absent from the rgpA mutant, KDP110. The VDS fraction from the rgpA rgpB mutant, KDP112, did not contain adhesin peptides.

    Journal: Infection and Immunity

    Article Title: Porphyromonas gingivalis Gingipains and Adhesion to Epithelial Cells

    doi: 10.1128/IAI.69.5.3048-3056.2001

    Figure Lengend Snippet: Gingipain adhesins in VDS fractions from P. gingivalis ATCC 33277 and rgp mutant strains. (A) VDS fractions from parent and mutant strains after SDS-PAGE and silver staining. (B) Western blot of the VDS fractions using a polyclonal antibody to gingipain adhesin domains. The 44-, 39-, 27-, and 19-kDa peptides in the fraction from ATCC 33277 are indicated by arrowheads. The 44-kDa peptide was absent from the rgpA mutant, KDP110. The VDS fraction from the rgpA rgpB mutant, KDP112, did not contain adhesin peptides.

    Article Snippet: Cognizant of a potential role for gingipains in adherence, we examined the effects of gingipain mutations on P. gingivalis ATCC 33277 adhesion to epithelial cell monolayers.

    Techniques: Mutagenesis, SDS Page, Silver Staining, Western Blot

    Gingipain-mediated adhesion and detachment of P. gingivalis to epithelial cells. We hypothesize that adhesion is mediated by gingipain adhesin peptides localized at the surface of the outer membrane and in membrane vesicles. Rgp catalytic activities, either noncovalently linked with adhesin domains or as soluble proteins, modulate adhesion through digestion of binding substrate. With the parent strain, ATCC 33277, the monolayer adhesion assay measures the net reaction of bacterial attachment to and detachment from receptors or extracellular matrix proteins at the epithelial cell surface. When catalytic activity is reduced by mutation (KDP112), the assay measures the large number of bacteria that attach (via Kgp or HagA adhesin peptides) and accumulate on the monolayers but do not detach or do so very slowly.

    Journal: Infection and Immunity

    Article Title: Porphyromonas gingivalis Gingipains and Adhesion to Epithelial Cells

    doi: 10.1128/IAI.69.5.3048-3056.2001

    Figure Lengend Snippet: Gingipain-mediated adhesion and detachment of P. gingivalis to epithelial cells. We hypothesize that adhesion is mediated by gingipain adhesin peptides localized at the surface of the outer membrane and in membrane vesicles. Rgp catalytic activities, either noncovalently linked with adhesin domains or as soluble proteins, modulate adhesion through digestion of binding substrate. With the parent strain, ATCC 33277, the monolayer adhesion assay measures the net reaction of bacterial attachment to and detachment from receptors or extracellular matrix proteins at the epithelial cell surface. When catalytic activity is reduced by mutation (KDP112), the assay measures the large number of bacteria that attach (via Kgp or HagA adhesin peptides) and accumulate on the monolayers but do not detach or do so very slowly.

    Article Snippet: Cognizant of a potential role for gingipains in adherence, we examined the effects of gingipain mutations on P. gingivalis ATCC 33277 adhesion to epithelial cell monolayers.

    Techniques: Binding Assay, Cell Adhesion Assay, Activity Assay, Mutagenesis

    Mean relative closure (±SEM) from all biological replicates of scratches in oral epithelial cells challenged with heat inactivated and viable P. gingivalis wild type strain ATCC 33277, ΔKgp, and ΔRgpArgpB mutants (a) and heat-inactivated and viable P. gingivalis wild-type strain W50, ΔKgp, and ΔRgpArgpB mutants and with addition of KYT-36 and KYT-1 peptides (b). *=significantly different from wild-type strain of the same MOI ( p

    Journal: Journal of Oral Microbiology

    Article Title: The impact of virulence factors of Porphyromonas gingivalis on wound healing in vitro

    doi: 10.3402/jom.v7.27543

    Figure Lengend Snippet: Mean relative closure (±SEM) from all biological replicates of scratches in oral epithelial cells challenged with heat inactivated and viable P. gingivalis wild type strain ATCC 33277, ΔKgp, and ΔRgpArgpB mutants (a) and heat-inactivated and viable P. gingivalis wild-type strain W50, ΔKgp, and ΔRgpArgpB mutants and with addition of KYT-36 and KYT-1 peptides (b). *=significantly different from wild-type strain of the same MOI ( p

    Article Snippet: Viable W83 and W50 inhibited cell migration more than their heat-inactivated variants (not statistically significant for W50 at MOI 10); however, no differences were found between heat-inactivated and viable P. gingivalis ATCC 33277 (p > 0.05).

    Techniques:

    Percentage closure of a scratch in oral epithelial cells challenged with P. gingivalis ATCC 33277 and medium only.

    Journal: Journal of Oral Microbiology

    Article Title: The impact of virulence factors of Porphyromonas gingivalis on wound healing in vitro

    doi: 10.3402/jom.v7.27543

    Figure Lengend Snippet: Percentage closure of a scratch in oral epithelial cells challenged with P. gingivalis ATCC 33277 and medium only.

    Article Snippet: Viable W83 and W50 inhibited cell migration more than their heat-inactivated variants (not statistically significant for W50 at MOI 10); however, no differences were found between heat-inactivated and viable P. gingivalis ATCC 33277 (p > 0.05).

    Techniques:

    Mean relative closure (±SEM) from all biological replicates of scratches in oral epithelial cells challenged with heat-inactivated and viable P. gingivalis strains ATCC 33277, W83, and W50. a=significantly different from control ( p

    Journal: Journal of Oral Microbiology

    Article Title: The impact of virulence factors of Porphyromonas gingivalis on wound healing in vitro

    doi: 10.3402/jom.v7.27543

    Figure Lengend Snippet: Mean relative closure (±SEM) from all biological replicates of scratches in oral epithelial cells challenged with heat-inactivated and viable P. gingivalis strains ATCC 33277, W83, and W50. a=significantly different from control ( p

    Article Snippet: Viable W83 and W50 inhibited cell migration more than their heat-inactivated variants (not statistically significant for W50 at MOI 10); however, no differences were found between heat-inactivated and viable P. gingivalis ATCC 33277 (p > 0.05).

    Techniques:

    Binding of MVs of P. gingivalis to SBs. Binding of P. gingivalis MVs to SBs was examined by SDS-PAGE (A: silver staining, B: WB of FimA). Five µg of the starting material from conventional purification (Crude) was incubated with same amount (20 mg, wet weight) of three SBs with different surface chemistries (SB-Epoxy, SB-COOH, and SB-NH 2 ). After incubating the crude MVs with SBs, unbound components were washed five times with PBS. The first and fifth unbound fractions are applied to the wells (Unbound, 1 st and Unbound, 5 th , or lanes 1 and 2). Then bound components were eluted by addition of SDS-PAGE loading buffer (Elu or lane 3). “MW” denotes the molecular weight marker. The same volume (10 µl) of samples was applied to each well of the PAGE gel. (B) Identical samples to those used in (A) were used for the WB of FimA. The location of FimA is shown by an arrow.

    Journal: PLoS ONE

    Article Title: A Novel Approach for Purification and Selective Capture of Membrane Vesicles of the Periodontopathic Bacterium, Porphyromonas gingivalis: Membrane Vesicles Bind to Magnetic Beads Coated with Epoxy Groups in a Noncovalent, Species-Specific Manner

    doi: 10.1371/journal.pone.0095137

    Figure Lengend Snippet: Binding of MVs of P. gingivalis to SBs. Binding of P. gingivalis MVs to SBs was examined by SDS-PAGE (A: silver staining, B: WB of FimA). Five µg of the starting material from conventional purification (Crude) was incubated with same amount (20 mg, wet weight) of three SBs with different surface chemistries (SB-Epoxy, SB-COOH, and SB-NH 2 ). After incubating the crude MVs with SBs, unbound components were washed five times with PBS. The first and fifth unbound fractions are applied to the wells (Unbound, 1 st and Unbound, 5 th , or lanes 1 and 2). Then bound components were eluted by addition of SDS-PAGE loading buffer (Elu or lane 3). “MW” denotes the molecular weight marker. The same volume (10 µl) of samples was applied to each well of the PAGE gel. (B) Identical samples to those used in (A) were used for the WB of FimA. The location of FimA is shown by an arrow.

    Article Snippet: We previously observed that P. gingivalis ATCC 33277 MVs prepared by ultracentrifugation contained many fimbriae .

    Techniques: Binding Assay, SDS Page, Silver Staining, Western Blot, Purification, Incubation, Molecular Weight, Marker, Polyacrylamide Gel Electrophoresis

    Binding to SB-Epoxy of MVs from different bacterial species. Shown are results of the assays using crude MV preparations from the following five different species: E. coli KP7600, N. meningitidis H44/76, P. aeruginosa PAO1 Holloway, C. perfringens strain 13, and P. gingivalis ATCC 33277. (A-D) Crude MVs from a single strain was incubated with the SB-Epoxy. Unbound components were collected in five washes and bound components were eventually eluted by SDS-PAGE loading buffer. Lanes denoted “1” are the starting material from conventional purification (Crude). Lanes denoted “2” are the first unbound fractions (Unbound, 1 st ). Lanes denoted “3” are the fifth unbound fractions (Unbound, 5 th ). Lanes denoted “4” are the elution fractions (Elu). (A: E. coli KP7600, B: N. meningitidis H44/76, C: P. aeruginosa PAO1-Tokai, D: C. perfringens strain 13). (E) Crude MVs from the five species were mixed with SB-Epoxy. The selective elimination of P. gingivalis MVs from the mixture is shown. Lanes 5 12: the input of mixtures of crude MVs (Crude mixture). Lanes 6 13: the first unbound fractions (Unbound, 1 st ). Lanes 12 13: five-fold diluted samples of lanes 5 6, respectively. Lanes 7, 8, 9: the third unbound fraction (Unbound, 3 rd ), the fifth unbound fraction (Unbound, 5 th ), and the elution fraction (Elu-Mix) from the mixture of four different MV preparations, respectively. Lane 10: the molecular weight marker. Lane 11: the crude MVs of P. gingivalis (Pg, Crude). Lane 14: the elution fraction from crude MVs of P. gingivalis only (Elu-Pg). The same volume (10 µl) was applied in each well of each PAGE gel. P. gingivalis FimA is denoted by an asterisk (*). N. meningitidis PorB is denoted by a dagger (†).

    Journal: PLoS ONE

    Article Title: A Novel Approach for Purification and Selective Capture of Membrane Vesicles of the Periodontopathic Bacterium, Porphyromonas gingivalis: Membrane Vesicles Bind to Magnetic Beads Coated with Epoxy Groups in a Noncovalent, Species-Specific Manner

    doi: 10.1371/journal.pone.0095137

    Figure Lengend Snippet: Binding to SB-Epoxy of MVs from different bacterial species. Shown are results of the assays using crude MV preparations from the following five different species: E. coli KP7600, N. meningitidis H44/76, P. aeruginosa PAO1 Holloway, C. perfringens strain 13, and P. gingivalis ATCC 33277. (A-D) Crude MVs from a single strain was incubated with the SB-Epoxy. Unbound components were collected in five washes and bound components were eventually eluted by SDS-PAGE loading buffer. Lanes denoted “1” are the starting material from conventional purification (Crude). Lanes denoted “2” are the first unbound fractions (Unbound, 1 st ). Lanes denoted “3” are the fifth unbound fractions (Unbound, 5 th ). Lanes denoted “4” are the elution fractions (Elu). (A: E. coli KP7600, B: N. meningitidis H44/76, C: P. aeruginosa PAO1-Tokai, D: C. perfringens strain 13). (E) Crude MVs from the five species were mixed with SB-Epoxy. The selective elimination of P. gingivalis MVs from the mixture is shown. Lanes 5 12: the input of mixtures of crude MVs (Crude mixture). Lanes 6 13: the first unbound fractions (Unbound, 1 st ). Lanes 12 13: five-fold diluted samples of lanes 5 6, respectively. Lanes 7, 8, 9: the third unbound fraction (Unbound, 3 rd ), the fifth unbound fraction (Unbound, 5 th ), and the elution fraction (Elu-Mix) from the mixture of four different MV preparations, respectively. Lane 10: the molecular weight marker. Lane 11: the crude MVs of P. gingivalis (Pg, Crude). Lane 14: the elution fraction from crude MVs of P. gingivalis only (Elu-Pg). The same volume (10 µl) was applied in each well of each PAGE gel. P. gingivalis FimA is denoted by an asterisk (*). N. meningitidis PorB is denoted by a dagger (†).

    Article Snippet: We previously observed that P. gingivalis ATCC 33277 MVs prepared by ultracentrifugation contained many fimbriae .

    Techniques: Binding Assay, Incubation, SDS Page, Purification, Molecular Weight, Marker, Polyacrylamide Gel Electrophoresis

    Endotoxin activity (endotoxin units (EU), mean ± SD) measured by Limulus amebocyte lysate assay of Escherichia coli (E.c.) LPS, Porphyromonas gingivalis (P.g.) LPS as well as P. gingivalis ATCC 33,277 and Aggregatibacter actinomycetemcomitans Y4, without and with 2 h pre-exposure to 0.01%, 0.25%, 1% taurolidine (TAU) and 16 µg/mL minocycline (MINO).* p

    Journal: Antibiotics

    Article Title: Taurolidine Acts on Bacterial Virulence Factors and Does Not Induce Resistance in Periodontitis-Associated Bacteria—An In-Vitro Study

    doi: 10.3390/antibiotics9040166

    Figure Lengend Snippet: Endotoxin activity (endotoxin units (EU), mean ± SD) measured by Limulus amebocyte lysate assay of Escherichia coli (E.c.) LPS, Porphyromonas gingivalis (P.g.) LPS as well as P. gingivalis ATCC 33,277 and Aggregatibacter actinomycetemcomitans Y4, without and with 2 h pre-exposure to 0.01%, 0.25%, 1% taurolidine (TAU) and 16 µg/mL minocycline (MINO).* p

    Article Snippet: Visualization of Taurolidine Action Differences of transmission electron micrographs (TEM) of P. gingivalis ATCC 33,277 and T. forsythia Be13237 without and with 2 h exposure to 1% taurolidine were small ( ).

    Techniques: Activity Assay

    Arginine specific amidolytic activity (Arg act) measured by Δ abs 405 nm after addition of taurolidine (TAU; a , c , e , g ) and minocycline (MINO; b , d , f , h ) in different concentrations to 10 nM RgpB ( a , b ), Porphyromonas gingivalis ATCC 33277 ( c , d ), P. gingivalis J374-1 ( e , f ) and P. gingivalis HG66 ( g , h ).

    Journal: Antibiotics

    Article Title: Taurolidine Acts on Bacterial Virulence Factors and Does Not Induce Resistance in Periodontitis-Associated Bacteria—An In-Vitro Study

    doi: 10.3390/antibiotics9040166

    Figure Lengend Snippet: Arginine specific amidolytic activity (Arg act) measured by Δ abs 405 nm after addition of taurolidine (TAU; a , c , e , g ) and minocycline (MINO; b , d , f , h ) in different concentrations to 10 nM RgpB ( a , b ), Porphyromonas gingivalis ATCC 33277 ( c , d ), P. gingivalis J374-1 ( e , f ) and P. gingivalis HG66 ( g , h ).

    Article Snippet: Visualization of Taurolidine Action Differences of transmission electron micrographs (TEM) of P. gingivalis ATCC 33,277 and T. forsythia Be13237 without and with 2 h exposure to 1% taurolidine were small ( ).

    Techniques: Activity Assay

    Transmission electron micrographs (TEM) images of Porphyromonas gingivalis ATCC 33277 and Tannerella forsythia Be13237 after treatment with 1% taurolidine for 2 h, in comparison to its control. P. gingivalis shows a decomposition of the cytoplasm leading to unstained areas (arrowheads). For T. forsythia , no difference could be found. Scale bars: 200 nm.

    Journal: Antibiotics

    Article Title: Taurolidine Acts on Bacterial Virulence Factors and Does Not Induce Resistance in Periodontitis-Associated Bacteria—An In-Vitro Study

    doi: 10.3390/antibiotics9040166

    Figure Lengend Snippet: Transmission electron micrographs (TEM) images of Porphyromonas gingivalis ATCC 33277 and Tannerella forsythia Be13237 after treatment with 1% taurolidine for 2 h, in comparison to its control. P. gingivalis shows a decomposition of the cytoplasm leading to unstained areas (arrowheads). For T. forsythia , no difference could be found. Scale bars: 200 nm.

    Article Snippet: Visualization of Taurolidine Action Differences of transmission electron micrographs (TEM) of P. gingivalis ATCC 33,277 and T. forsythia Be13237 without and with 2 h exposure to 1% taurolidine were small ( ).

    Techniques: Transmission Assay, Transmission Electron Microscopy

    Distribution of PAD in various strains of P. gingivalis . The production of citrulline from BAEE was determined in cellular (open bar), supernatant (solid bar), and vesicle (hatched bar) fractions from HG66, ATCC 53978 (W50), and ATCC 33277 strains of P. gingivalis .

    Journal: Infection and Immunity

    Article Title: Purification, Characterization, and Sequence Analysis of a Potential Virulence Factor from Porphyromonas gingivalis, Peptidylarginine Deiminase

    doi:

    Figure Lengend Snippet: Distribution of PAD in various strains of P. gingivalis . The production of citrulline from BAEE was determined in cellular (open bar), supernatant (solid bar), and vesicle (hatched bar) fractions from HG66, ATCC 53978 (W50), and ATCC 33277 strains of P. gingivalis .

    Article Snippet: Culture fluid (1,500 ml) from 36 h of cultivation of P. gingivalis ATCC 53978 (W50) and ATCC 33277 was obtained by centrifugation of cells (6,000 × g ; 30 min; 4°C).

    Techniques: