Journal: iScience

Article Title: TZAP overexpression induces telomere dysfunction and ALT-like activity in ATRX/DAXX-deficient cells

doi: 10.1016/j.isci.2023.106405

Figure Lengend Snippet: Key resources table

Article Snippet: 8 hours later, the media of the cells was exchanged for antibiotic-containing media and cells were either transfected again or harvested for experiments 72 hours later. . Antibody sources α-GAPDH (# PAB17013 , Abnova), α-γH2A.x Ser 139 (JBW301, #05-636, Millipore), α-Myc-tag (9B11, #2276S, Cell Signaling), α-p-Chk1 Ser 345 (133D3, #2348S, Cell Signaling), α-PML (#sc-5621, Santa Cruz) α-p-Chk2 Thr 68 (#2661S, Cell Signaling), α-p-ATR Thr 1989 (#ab223258, Abcam), α-p-ATM Ser 1981 (D25E5, #13050S, Cell Signaling), α-ATRX (#sc-15408, Santa Cruz), α-DAXX (#sc-7152, Santa Cruz), α-H3.3 (#ab176840, Abcam), α-RMI1 (#NB100-1720, Novus), α-FLAG-tag (M2, #F1804-1MG, Sigma), α-RPA32 (9H8, #ab2175, Abcam), α-RPA70 (#sc-28304, Santa Cruz). α-BLM (R7019), α-TRF1 (R6839) and α-TRF2 (R6841) were raised in our lab. All antibodies were used for Western Blotting apart from α-BLM, α-Myc and α-FLAG that were also used for IF and α-RPA32, α-RPA70, α-PML, α-TRF1 and α-TRF2 that were used for IF only.

Techniques: Recombinant, Modification, Transfection, Electrophoresis, Blocking Assay, Imaging, Purification, Software