Journal: Oncotarget

Article Title: Radiosensitization of the PI3K inhibitor HS-173 through reduction of DNA damage repair in pancreatic cancer

doi: 10.18632/oncotarget.22850

Figure Lengend Snippet: (A) Miapaca-2 cells were treated with HS-173 (0-10 μM) for 6 h before irradiation (10 Gy). After 24 h, cells were stained with PI and analyzed by flow cytometry. Quantitative PI staining data is presented as a percentage of the cell cycle distribution. (B) Miapaca-2 cells were treated with HS-173 for 6 h before irradiation (10 Gy). After 24 h, p-Cdc2 levels were assayed by Western blotting. Data are expressed as means ± S.D. from three experiments ( *** P < 0.001).

Article Snippet: Primary monoclonal antibodies against the following proteins were used: cleaved PARP, cleaved caspase-3, survivin, p-AKT, AKT (Cell Signaling Technology, MA, USA) p-Cdc2, p-ATM, ATM, p-DNA-PKcs, DNA-PKcs, p-KAP1, p-53BP1 (Abcam, Cambridge, UK) KAP1, 53BP1(Santa Cruz, Dallas, TX, USA) and β-actin (Sigma-Aldrich, OH, USA) Secondary antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Irradiation, Staining, Flow Cytometry, Western Blot

Journal: Acta Pharmacologica Sinica

Article Title: LW-213, a newly synthesized flavonoid, induces G2/M phase arrest and apoptosis in chronic myeloid leukemia

doi: 10.1038/s41401-019-0270-4

Figure Lengend Snippet: LW-213 induced G2/M phase arrest in CML cell lines. a K562 and K562r cells were treated with the indicated concentrations of LW-213 for 12 and 24 h. Cell cycle arrest was determined by PI staining. b , c After treatment with LW-213, the proportions of cells in each cell cycle phase were summarized. Data represent the mean ± S.E.M. from three independent experiments. Asterisks denote statistically significant differences compared with untreated cells (* P < 0.05, ** P < 0.01). d , e After treatment with 0 and 15 μM LW-213 for 24 h, K562 and K562r cells were stained with β-tubulin antibody (labeled with Alexa Fluor 488 goat anti-rabbit lgG antibody; green fluorescence) and PI (red fluorescence). The number of cells in M phase was quantified in three experiments, with 200 cells per condition. Asterisks denote statistically significant differences compared with untreated cells (* P < 0.05). f , g Levels of Cyclin B1, CDC2 and p-CDC2 in K562 and K562r cells were analyzed by Western blots after treatment with LW-213 for 12 and 24 h, respectively. β-Actin was used as a loading control. The results are representative of three independent experiments. Data represent the mean ± S.E.M. from three independent experiments. Asterisks denote statistically significant differences compared with untreated cells (* P < 0.05, ** P < 0.01)

Article Snippet: Primary antibodies for Cyclin B1, CDC2, p-CDC2 (Y15), β-tubulin, MCL-1, p-CDK9 (Thr186), AKT, p-AKT (Ser473), β-actin, BCL-2, STAT5, STAT3, p-STAT5 (Y694), p-RNAPII-S2, p-RNAPII-S5, ABL1, LC3, P62/SQSTM1, caspase 3, and caspase 9 were obtained from ABclonal Technology (Wuhan, China).

Techniques: Staining, Labeling, Fluorescence, Western Blot