orbitrap fusion tribrid Search Results


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  • 99
    Thermo Fisher orbitrap fusion tribid mass spectrometer
    Proteomic analysis of tryptic peptides from (a) expressed archetype and splice variants of human OGDH E1 and (b) OGDH E1 separated from rat brain and heart mitochondria. Results are shown as PSM for the indicated peptide or the mean of peptides a and b expressed as percentages of the total E1 protein PSM and are means ± S.E.M. for three or four observations. Values of total E1 protein PSM were archetype 3571 ± 552 ( n = 4), LS1 3719 ± 552 ( n = 3), Insert 3782 ± 1961 ( n = 3), LS1/Insert 3407 ± 156 ( n = 3), rat brain OGDH E1 620 ± 126 ( n = 4) and rat heart OGDH E1 1086 ± 126 ( n = 4). All observations were obtained using an <t>Orbitrap</t> Fusion <t>Tribrid</t> mass spectrometer. The number of PSM is a measure of the amount of a peptide.
    Orbitrap Fusion Tribid Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2094 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2094 article reviews
    Price from $9.99 to $1999.99
    orbitrap fusion tribid mass spectrometer - by Bioz Stars, 2020-04
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    99
    Thermo Fisher orbitrap fusion lumos tribrid
    Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by <t>Orbitrap</t> Fusion <t>Lumos</t> ( b ).
    Orbitrap Fusion Lumos Tribrid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap fusion lumos tribrid/product/Thermo Fisher
    Average 99 stars, based on 216 article reviews
    Price from $9.99 to $1999.99
    orbitrap fusion lumos tribrid - by Bioz Stars, 2020-04
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    94
    Thermo Fisher ltq orbitrap fusion tribrid
    Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by <t>Orbitrap</t> Fusion <t>Lumos</t> ( b ).
    Ltq Orbitrap Fusion Tribrid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 4 article reviews
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    91
    Thermo Fisher etd enabled orbitrap fusion tribrid instrument
    Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by <t>Orbitrap</t> Fusion <t>Lumos</t> ( b ).
    Etd Enabled Orbitrap Fusion Tribrid Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 3 article reviews
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    etd enabled orbitrap fusion tribrid instrument - by Bioz Stars, 2020-04
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    80
    Thermo Fisher orbitrap fusion lumos tribrid mass spectrometers
    Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by <t>Orbitrap</t> Fusion <t>Lumos</t> ( b ).
    Orbitrap Fusion Lumos Tribrid Mass Spectrometers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap fusion lumos tribrid mass spectrometers/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    80
    Thermo Fisher orbitrap fusion tribrid high resolution mass spectrometer
    Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by <t>Orbitrap</t> Fusion <t>Lumos</t> ( b ).
    Orbitrap Fusion Tribrid High Resolution Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap fusion tribrid high resolution mass spectrometer/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
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    orbitrap fusion tribrid high resolution mass spectrometer - by Bioz Stars, 2020-04
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    93
    Thermo Fisher orbirtrap fusion lumos tribrid
    Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by <t>Orbitrap</t> Fusion <t>Lumos</t> ( b ).
    Orbirtrap Fusion Lumos Tribrid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    orbirtrap fusion lumos tribrid - by Bioz Stars, 2020-04
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    94
    Thermo Fisher orbitrap fusion tribid lumos
    Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by <t>Orbitrap</t> Fusion <t>Lumos</t> ( b ).
    Orbitrap Fusion Tribid Lumos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap fusion tribid lumos/product/Thermo Fisher
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    orbitrap fusion tribid lumos - by Bioz Stars, 2020-04
    94/100 stars
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    Image Search Results


    Proteomic analysis of tryptic peptides from (a) expressed archetype and splice variants of human OGDH E1 and (b) OGDH E1 separated from rat brain and heart mitochondria. Results are shown as PSM for the indicated peptide or the mean of peptides a and b expressed as percentages of the total E1 protein PSM and are means ± S.E.M. for three or four observations. Values of total E1 protein PSM were archetype 3571 ± 552 ( n = 4), LS1 3719 ± 552 ( n = 3), Insert 3782 ± 1961 ( n = 3), LS1/Insert 3407 ± 156 ( n = 3), rat brain OGDH E1 620 ± 126 ( n = 4) and rat heart OGDH E1 1086 ± 126 ( n = 4). All observations were obtained using an Orbitrap Fusion Tribrid mass spectrometer. The number of PSM is a measure of the amount of a peptide.

    Journal: The Biochemical journal

    Article Title: Calcium-insensitive splice variants of mammalian E1 subunit of 2-oxoglutarate dehydrogenase complex with tissue-specific patterns of expression

    doi: 10.1042/BCJ20160135

    Figure Lengend Snippet: Proteomic analysis of tryptic peptides from (a) expressed archetype and splice variants of human OGDH E1 and (b) OGDH E1 separated from rat brain and heart mitochondria. Results are shown as PSM for the indicated peptide or the mean of peptides a and b expressed as percentages of the total E1 protein PSM and are means ± S.E.M. for three or four observations. Values of total E1 protein PSM were archetype 3571 ± 552 ( n = 4), LS1 3719 ± 552 ( n = 3), Insert 3782 ± 1961 ( n = 3), LS1/Insert 3407 ± 156 ( n = 3), rat brain OGDH E1 620 ± 126 ( n = 4) and rat heart OGDH E1 1086 ± 126 ( n = 4). All observations were obtained using an Orbitrap Fusion Tribrid mass spectrometer. The number of PSM is a measure of the amount of a peptide.

    Article Snippet: The number of peptide spectral matches (PSM) can be used as a measure of the amount of a particular peptide [ – ]. shows the values of PSM for the various relevant peptides with mass/charge ratios in the range 300–2000 which were detected in studies using the Orbitrap Fusion Tribrid mass spectrometer on samples of expressed human archetype and the three splice variants.

    Techniques: Mass Spectrometry

    Overview of the complex multi-system pathophysiology of dystrophinopathy and proteomic workflow to analyse the mdx - 4cv liver. Duchenne muscular dystrophy is caused by primary abnormalities in dystrophin and triggers progressive skeletal muscle wasting, cardio-respiratory abnormalities and cognitive impairments. In addition, X-linked muscular dystrophy is also characterized by secondary effects on a variety of organ systems including the liver. Proteome-wide changes in liver tissue were determined by comparative proteomics using the dystrophic mdx - 4cv mouse model of Duchenne muscular dystrophy. Results obtained by mass spectrometry using an Orbitrap Fusion Tribrid apparatus were analysed by systems bioinformatics, and key findings were confirmed by verification studies employing immunoblotting and immunofluorescence microscopy

    Journal: Clinical Proteomics

    Article Title: Proteomic profiling of liver tissue from the mdx-4cv mouse model of Duchenne muscular dystrophy

    doi: 10.1186/s12014-018-9212-2

    Figure Lengend Snippet: Overview of the complex multi-system pathophysiology of dystrophinopathy and proteomic workflow to analyse the mdx - 4cv liver. Duchenne muscular dystrophy is caused by primary abnormalities in dystrophin and triggers progressive skeletal muscle wasting, cardio-respiratory abnormalities and cognitive impairments. In addition, X-linked muscular dystrophy is also characterized by secondary effects on a variety of organ systems including the liver. Proteome-wide changes in liver tissue were determined by comparative proteomics using the dystrophic mdx - 4cv mouse model of Duchenne muscular dystrophy. Results obtained by mass spectrometry using an Orbitrap Fusion Tribrid apparatus were analysed by systems bioinformatics, and key findings were confirmed by verification studies employing immunoblotting and immunofluorescence microscopy

    Article Snippet: Label-free liquid chromatography mass spectrometry Reverse-phased capillary high pressure liquid chromatography was carried out using the UltiMate 3000 nano system (Thermo Scientific) coupled directly in-line with the Thermo Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Scientific).

    Techniques: Mass Spectrometry, Immunofluorescence, Microscopy

    Metabolic Labeling and Workflow. ( A ) Glucose, acetate, fatty acids, and amino acids produce acetyl-CoA for use in acetylating cytoplasmic and nuclear proteins. The thicker arrows indicate that glucose contributes more to the production of acetyl-coA that subsequently acetylates proteins, compared to acetate. ( B ) The workflow consisted of growing HeLa cells in heavy-labeled media, collecting samples at eight time points, lysing the cells, digesting the proteins, enriching for acetylated peptides, and analyzing the peptides by mass spectrometry. The Orbitrap image is adapted from Thermo Fisher Scientific 56 . The cartoon cell matter and lab equipment were slightly modified from Servier Medical Art 57 .

    Journal: Scientific Reports

    Article Title: Proteome-wide acetylation dynamics in human cells

    doi: 10.1038/s41598-017-09918-3

    Figure Lengend Snippet: Metabolic Labeling and Workflow. ( A ) Glucose, acetate, fatty acids, and amino acids produce acetyl-CoA for use in acetylating cytoplasmic and nuclear proteins. The thicker arrows indicate that glucose contributes more to the production of acetyl-coA that subsequently acetylates proteins, compared to acetate. ( B ) The workflow consisted of growing HeLa cells in heavy-labeled media, collecting samples at eight time points, lysing the cells, digesting the proteins, enriching for acetylated peptides, and analyzing the peptides by mass spectrometry. The Orbitrap image is adapted from Thermo Fisher Scientific 56 . The cartoon cell matter and lab equipment were slightly modified from Servier Medical Art 57 .

    Article Snippet: Acetylated peptides were then enriched using anti-acetyl-lysine antibodies and run on nano liquid chromatography coupled online with tandem mass spectrometry (nanoLC-MS/MS) on an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific), which allows for detection of isotope incorporation into acetylated proteins.

    Techniques: Labeling, Mass Spectrometry, Modification

    Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by Orbitrap Fusion Lumos ( b ).

    Journal: Scientific Reports

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model

    doi: 10.1038/s41598-019-50301-1

    Figure Lengend Snippet: Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by Orbitrap Fusion Lumos ( b ).

    Article Snippet: Peptides were analyzed with an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA).

    Techniques:

    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the Orbitrap Fusion Lumos. (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.

    Journal: Journal of Proteome Research

    Article Title: A Study into the ADP-Ribosylome of IFN-γ-Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation

    doi: 10.1021/acs.jproteome.8b00895

    Figure Lengend Snippet: Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the Orbitrap Fusion Lumos. (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.

    Article Snippet: LC–MS/MS Analysis All peptide samples were analyzed on an Orbitrap Fusion Lumos mass spectrometer fronted with an EASY-Spray Source, coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific).

    Techniques: Injection

    General sample preparation and data acquisition workflow: N-glycans were released by FANGS, labeled with 6-plex aminoxyTMT, combined and cleaned up with an Oasis HLB cartridge, and analyzed on an LC-Tribrid quadrupole-ion trap-Orbitrap MS platform.

    Journal: Analytical chemistry

    Article Title: Targeted MultiNotch MS3 Approach for Relative Quantification of N-Glycans Using Multiplexed Carbonyl-Reactive Isobaric Tags

    doi: 10.1021/acs.analchem.7b03289

    Figure Lengend Snippet: General sample preparation and data acquisition workflow: N-glycans were released by FANGS, labeled with 6-plex aminoxyTMT, combined and cleaned up with an Oasis HLB cartridge, and analyzed on an LC-Tribrid quadrupole-ion trap-Orbitrap MS platform.

    Article Snippet: An Orbitrap Fusion Lumos Tribrid quadrupole-ion trap-Orbitrap mass spectrometer with NanoSpray Flex ion source (Thermo Scientific, Bremen, Germany) was used for data acquisition.

    Techniques: Sample Prep, Labeling, Mass Spectrometry