orbitrap fusion instrument Search Results


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  • 90
    Thermo Fisher orbitrap fusion tribrid mass spectrometer
    Experiment workflow. A) Control and AD human postmortem frontal cortex brain tissues (n=5 each group) were homogenized in 8M urea lysis buffer. For both global and ubiquitylome analysis, brain protein extracts from each sample was first denatured, alkylated and proteolytically digested followed by peptide desalting as described in methods. For global proteome analysis, peptides were analyzed by LC-MS/MS on <t>Orbitrap</t> Fusion <t>Tribrid</t> mass spectrometer. For brain ubiquitylome analysis, peptides were subjected to di-Gly affinity enrichment as described in methods followed by LC-MS/MS analysis in replicate on an Orbitrap Fusion Tribrid mass spectrometer. Protein abundance was calculated by peptide ion-intensity measurements across LC-MS runs using the label free quantification (LFQ) algorithm in MaxQuant.
    Orbitrap Fusion Tribrid Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2566 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap fusion tribrid mass spectrometer/product/Thermo Fisher
    Average 90 stars, based on 2566 article reviews
    Price from $9.99 to $1999.99
    orbitrap fusion tribrid mass spectrometer - by Bioz Stars, 2020-02
    90/100 stars
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    99
    Thermo Fisher orbitrap fusion instrument
    Tandem MS (EThcD) of an AGP middle-down glycopeptide on an <t>Orbitrap</t> Fusion instrument (precursor m/z : 1302.4178, [M+7H] 7+ ). ETD reaction time was 8.75 msec and HCD normalized collision energy was set at 20 for this scan. C* = Carbamidomethyl Cysteine.
    Orbitrap Fusion Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap fusion instrument/product/Thermo Fisher
    Average 99 stars, based on 108 article reviews
    Price from $9.99 to $1999.99
    orbitrap fusion instrument - by Bioz Stars, 2020-02
    99/100 stars
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    75
    Thermo Fisher instrument orbitrap fusion lumos
    MS/MS spectra of sequences generated on <t>Orbitrap</t> Fusion <t>Lumos</t> under ETD mode.
    Instrument Orbitrap Fusion Lumos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/instrument orbitrap fusion lumos/product/Thermo Fisher
    Average 75 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    instrument orbitrap fusion lumos - by Bioz Stars, 2020-02
    75/100 stars
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    82
    Thermo Fisher fusion tribrid orbitrap instrument
    MS/MS spectra of sequences generated on <t>Orbitrap</t> Fusion <t>Lumos</t> under ETD mode.
    Fusion Tribrid Orbitrap Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fusion tribrid orbitrap instrument/product/Thermo Fisher
    Average 82 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    fusion tribrid orbitrap instrument - by Bioz Stars, 2020-02
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    78
    Thermo Fisher etd enabled orbitrap fusion tribrid instrument
    MS/MS spectra of sequences generated on <t>Orbitrap</t> Fusion <t>Lumos</t> under ETD mode.
    Etd Enabled Orbitrap Fusion Tribrid Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/etd enabled orbitrap fusion tribrid instrument/product/Thermo Fisher
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    etd enabled orbitrap fusion tribrid instrument - by Bioz Stars, 2020-02
    78/100 stars
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    90
    Thermo Fisher orbitrap fusion lumos mass spectrometer
    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the <t>Orbitrap</t> Fusion <t>Lumos.</t> (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.
    Orbitrap Fusion Lumos Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap fusion lumos mass spectrometer/product/Thermo Fisher
    Average 90 stars, based on 966 article reviews
    Price from $9.99 to $1999.99
    orbitrap fusion lumos mass spectrometer - by Bioz Stars, 2020-02
    90/100 stars
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    79
    Thermo Fisher orbitrap fusion lc ms ms instrumentation platform
    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the <t>Orbitrap</t> Fusion <t>Lumos.</t> (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.
    Orbitrap Fusion Lc Ms Ms Instrumentation Platform, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap fusion lc ms ms instrumentation platform/product/Thermo Fisher
    Average 79 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    orbitrap fusion lc ms ms instrumentation platform - by Bioz Stars, 2020-02
    79/100 stars
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    79
    Thermo Fisher orbitrap fusion device
    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the <t>Orbitrap</t> Fusion <t>Lumos.</t> (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.
    Orbitrap Fusion Device, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap fusion device/product/Thermo Fisher
    Average 79 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    orbitrap fusion device - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    90
    Thermo Fisher quadrupole orbitrap mass spectrometer
    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the <t>Orbitrap</t> Fusion <t>Lumos.</t> (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.
    Quadrupole Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 600 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quadrupole orbitrap mass spectrometer/product/Thermo Fisher
    Average 90 stars, based on 600 article reviews
    Price from $9.99 to $1999.99
    quadrupole orbitrap mass spectrometer - by Bioz Stars, 2020-02
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    90
    Thermo Fisher thermo q exactive plus mass spectrometer
    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the <t>Orbitrap</t> Fusion <t>Lumos.</t> (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.
    Thermo Q Exactive Plus Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermo q exactive plus mass spectrometer/product/Thermo Fisher
    Average 90 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    thermo q exactive plus mass spectrometer - by Bioz Stars, 2020-02
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    90
    Thermo Fisher ultimate 3000 rslcnano system
    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the <t>Orbitrap</t> Fusion <t>Lumos.</t> (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.
    Ultimate 3000 Rslcnano System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1856 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimate 3000 rslcnano system/product/Thermo Fisher
    Average 90 stars, based on 1856 article reviews
    Price from $9.99 to $1999.99
    ultimate 3000 rslcnano system - by Bioz Stars, 2020-02
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    90
    Thermo Fisher ultimate 3000 nanoflow lc system
    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the <t>Orbitrap</t> Fusion <t>Lumos.</t> (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.
    Ultimate 3000 Nanoflow Lc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimate 3000 nanoflow lc system/product/Thermo Fisher
    Average 90 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    ultimate 3000 nanoflow lc system - by Bioz Stars, 2020-02
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    Image Search Results


    Experiment workflow. A) Control and AD human postmortem frontal cortex brain tissues (n=5 each group) were homogenized in 8M urea lysis buffer. For both global and ubiquitylome analysis, brain protein extracts from each sample was first denatured, alkylated and proteolytically digested followed by peptide desalting as described in methods. For global proteome analysis, peptides were analyzed by LC-MS/MS on Orbitrap Fusion Tribrid mass spectrometer. For brain ubiquitylome analysis, peptides were subjected to di-Gly affinity enrichment as described in methods followed by LC-MS/MS analysis in replicate on an Orbitrap Fusion Tribrid mass spectrometer. Protein abundance was calculated by peptide ion-intensity measurements across LC-MS runs using the label free quantification (LFQ) algorithm in MaxQuant.

    Journal: Proteomics

    Article Title: Quantitative Analysis of the Brain Ubiquitylome in Alzheimer’s Disease

    doi: 10.1002/pmic.201800108

    Figure Lengend Snippet: Experiment workflow. A) Control and AD human postmortem frontal cortex brain tissues (n=5 each group) were homogenized in 8M urea lysis buffer. For both global and ubiquitylome analysis, brain protein extracts from each sample was first denatured, alkylated and proteolytically digested followed by peptide desalting as described in methods. For global proteome analysis, peptides were analyzed by LC-MS/MS on Orbitrap Fusion Tribrid mass spectrometer. For brain ubiquitylome analysis, peptides were subjected to di-Gly affinity enrichment as described in methods followed by LC-MS/MS analysis in replicate on an Orbitrap Fusion Tribrid mass spectrometer. Protein abundance was calculated by peptide ion-intensity measurements across LC-MS runs using the label free quantification (LFQ) algorithm in MaxQuant.

    Article Snippet: Peptides were analyzed on an Orbitrap Fusion Tribrid Mass Spectrometer (ThermoFisher Scientific).

    Techniques: Lysis, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Tandem MS (EThcD) of an AGP middle-down glycopeptide on an Orbitrap Fusion instrument (precursor m/z : 1302.4178, [M+7H] 7+ ). ETD reaction time was 8.75 msec and HCD normalized collision energy was set at 20 for this scan. C* = Carbamidomethyl Cysteine.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Comparison of collisional and electron-based dissociation modes for middle-down analysis of multiply glycosylated peptides

    doi: 10.1007/s13361-018-1909-y

    Figure Lengend Snippet: Tandem MS (EThcD) of an AGP middle-down glycopeptide on an Orbitrap Fusion instrument (precursor m/z : 1302.4178, [M+7H] 7+ ). ETD reaction time was 8.75 msec and HCD normalized collision energy was set at 20 for this scan. C* = Carbamidomethyl Cysteine.

    Article Snippet: HCD and EThcD (Higher-energy Collisional Dissociation and Electron Transfer Dissociation supplemental collisional activation) ( – ) experiments were performed on ZIC-HILIC-enriched and unfractionated bottom-up and middle-down glycopeptide samples by LC-MS using an EASY-nLC chromatograph with an EASY-Spray C18 LC column on a Thermo Orbitrap Fusion™ instrument.

    Techniques: Mass Spectrometry

    MS/MS spectra of sequences generated on Orbitrap Fusion Lumos under ETD mode.

    Journal: EuPA Open Proteomics

    Article Title: Quick and clean: Cracking sentences encoded in E. coli by LC–MS/MS, de novo sequencing, and dictionary search

    doi: 10.1016/j.euprot.2019.07.010

    Figure Lengend Snippet: MS/MS spectra of sequences generated on Orbitrap Fusion Lumos under ETD mode.

    Article Snippet: At the same time, we decided to analyze the sample on a different instrument – Orbitrap Fusion Lumos (Thermo Fisher) – to take advantage of multiple fragmentation modes (CID, HCD, ETD and EThcD) since we had plenty of sample left.

    Techniques: Mass Spectrometry, Generated

    MS/MS spectra of sequences generated on Orbitrap Fusion Lumos under CID mode and on Q Exactive HF-X under HCD mode.

    Journal: EuPA Open Proteomics

    Article Title: Quick and clean: Cracking sentences encoded in E. coli by LC–MS/MS, de novo sequencing, and dictionary search

    doi: 10.1016/j.euprot.2019.07.010

    Figure Lengend Snippet: MS/MS spectra of sequences generated on Orbitrap Fusion Lumos under CID mode and on Q Exactive HF-X under HCD mode.

    Article Snippet: At the same time, we decided to analyze the sample on a different instrument – Orbitrap Fusion Lumos (Thermo Fisher) – to take advantage of multiple fragmentation modes (CID, HCD, ETD and EThcD) since we had plenty of sample left.

    Techniques: Mass Spectrometry, Generated

    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the Orbitrap Fusion Lumos. (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.

    Journal: Journal of Proteome Research

    Article Title: A Study into the ADP-Ribosylome of IFN-γ-Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation

    doi: 10.1021/acs.jproteome.8b00895

    Figure Lengend Snippet: Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the Orbitrap Fusion Lumos. (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.

    Article Snippet: LC–MS/MS Analysis All peptide samples were analyzed on an Orbitrap Fusion Lumos mass spectrometer fronted with an EASY-Spray Source, coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific).

    Techniques: Injection