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    Thermo Fisher exactive orbitrap
    (a) The arrival time distribution of 1+ ions from Pierce calibration mixture utilizing SA mode (see text). The scan gate duration was 200 μ s, while the scan gate sweep rate was 200 μ <t>s/Orbitrap</t> scan. The m/z values of few ions are annotated. (b) Mass spectrum collected at Orbitrap scan number 92. (c) Mass spectrum collected at Orbitrap scan number 178.
    Exactive Orbitrap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 678 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (a) The arrival time distribution of 1+ ions from Pierce calibration mixture utilizing SA mode (see text). The scan gate duration was 200 μ s, while the scan gate sweep rate was 200 μ s/Orbitrap scan. The m/z values of few ions are annotated. (b) Mass spectrum collected at Orbitrap scan number 92. (c) Mass spectrum collected at Orbitrap scan number 178.

    Journal: Analytical chemistry

    Article Title: Development of an Ion Mobility Spectrometry-Orbitrap Mass Spectrometer Platform

    doi: 10.1021/acs.analchem.6b03027

    Figure Lengend Snippet: (a) The arrival time distribution of 1+ ions from Pierce calibration mixture utilizing SA mode (see text). The scan gate duration was 200 μ s, while the scan gate sweep rate was 200 μ s/Orbitrap scan. The m/z values of few ions are annotated. (b) Mass spectrum collected at Orbitrap scan number 92. (c) Mass spectrum collected at Orbitrap scan number 178.

    Article Snippet: Experiments were performed using a home-built IMS drift tube (resolving power ~73) that was integrated with an Exactive Orbitrap MS (Thermo Fisher Scientific, San Jose, CA).

    Techniques:

    Timing sequence for the IMS-Orbitrap MS in (a–d) SA, (e–h) SM, and (i–l) DM IMS modes of operation. The delay time, Δ t , between the scan gate and the IFT exit gate is stepped to collect an ion mobility spectrum.

    Journal: Analytical chemistry

    Article Title: Development of an Ion Mobility Spectrometry-Orbitrap Mass Spectrometer Platform

    doi: 10.1021/acs.analchem.6b03027

    Figure Lengend Snippet: Timing sequence for the IMS-Orbitrap MS in (a–d) SA, (e–h) SM, and (i–l) DM IMS modes of operation. The delay time, Δ t , between the scan gate and the IFT exit gate is stepped to collect an ion mobility spectrum.

    Article Snippet: Experiments were performed using a home-built IMS drift tube (resolving power ~73) that was integrated with an Exactive Orbitrap MS (Thermo Fisher Scientific, San Jose, CA).

    Techniques: Sequencing, Mass Spectrometry

    IMS-Orbitrap data for a vacuum and hydrotreated gas oil sample. Homologous series are noted on some of the trend lines.

    Journal: Analytical chemistry

    Article Title: Development of an Ion Mobility Spectrometry-Orbitrap Mass Spectrometer Platform

    doi: 10.1021/acs.analchem.6b03027

    Figure Lengend Snippet: IMS-Orbitrap data for a vacuum and hydrotreated gas oil sample. Homologous series are noted on some of the trend lines.

    Article Snippet: Experiments were performed using a home-built IMS drift tube (resolving power ~73) that was integrated with an Exactive Orbitrap MS (Thermo Fisher Scientific, San Jose, CA).

    Techniques:

    MS spectra (mass range: m/z 200–1000) by Orbitrap and MS/MS spectra (parent ion: m/z 316.1, mass range: 297.0–299.0 and 240.0–242.0) by TSQ for blank paper substrates subject to different treatments. (a) untreated 31 ET paper,

    Journal: The Analyst

    Article Title: Quantitative Paper Spray Mass Spectrometry Analysis of Drugs of Abuse

    doi: 10.1039/c3an00934c

    Figure Lengend Snippet: MS spectra (mass range: m/z 200–1000) by Orbitrap and MS/MS spectra (parent ion: m/z 316.1, mass range: 297.0–299.0 and 240.0–242.0) by TSQ for blank paper substrates subject to different treatments. (a) untreated 31 ET paper,

    Article Snippet: The MS spectra were recorded using an Exactive Orbitrap mass spectrometer (Exactive, Thermo Scientific, CA).

    Techniques: Mass Spectrometry

    Workflow of TMT quantitative shotgun proteomic analysis. Proteins extracted from rice leaves were reduced, alkylased, precipitated and digested before being labelled with Tandem Mass Tags. Labelled samples were pooled, fractionated by SCX and then analysed on a Q Exactive Orbitrap mass spectrometer. Raw data generated from the mass spectrometer were processed using Proteome Discover v1.4 and Mascot. The search results were further analysed by the TMTPrePro software package.

    Journal: Data in Brief

    Article Title: Label-free and isobaric tandem mass tag (TMT) multiplexed quantitative proteomic data of two contrasting rice cultivars exposed to drought stress and recovery

    doi: 10.1016/j.dib.2018.12.041

    Figure Lengend Snippet: Workflow of TMT quantitative shotgun proteomic analysis. Proteins extracted from rice leaves were reduced, alkylased, precipitated and digested before being labelled with Tandem Mass Tags. Labelled samples were pooled, fractionated by SCX and then analysed on a Q Exactive Orbitrap mass spectrometer. Raw data generated from the mass spectrometer were processed using Proteome Discover v1.4 and Mascot. The search results were further analysed by the TMTPrePro software package.

    Article Snippet: These fractions were desalted using C18 OMIX® tips (Agilent) and analysed on a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) coupled to an EASY-nLC1000 (Thermo Scientific).

    Techniques: Mass Spectrometry, Generated, Software

    Overlay of extracted ion chromatograms (EICs) of HT2/T2 plant metabolites. ( a ) Overlaid EICs of HT2 metabolites based on HT2-treated oat sample (time point full-ripening) and Table 1 ; ( b ) overlaid EICs of T2 metabolites based on T2-treated oat samples (time point full-ripening and accumulated time points marked with an asterisk) and Table 2 . Oat panicles were treated with a 1/1 mixture of non-labelled and uniformly 13 C-labelled toxin, extracted and analysed by LC-Orbitrap-MS in positive and negative ion mode and MetExtract II software. Non-labelled metabolite form is depicted with positive intensity (up) and corresponding 13 C-labelled metabolite form with negative intensity (down). HT2, HT-2 toxin; T2, T-2 toxin.

    Journal: Toxins

    Article Title: Metabolism of HT-2 Toxin and T-2 Toxin in Oats

    doi: 10.3390/toxins8120364

    Figure Lengend Snippet: Overlay of extracted ion chromatograms (EICs) of HT2/T2 plant metabolites. ( a ) Overlaid EICs of HT2 metabolites based on HT2-treated oat sample (time point full-ripening) and Table 1 ; ( b ) overlaid EICs of T2 metabolites based on T2-treated oat samples (time point full-ripening and accumulated time points marked with an asterisk) and Table 2 . Oat panicles were treated with a 1/1 mixture of non-labelled and uniformly 13 C-labelled toxin, extracted and analysed by LC-Orbitrap-MS in positive and negative ion mode and MetExtract II software. Non-labelled metabolite form is depicted with positive intensity (up) and corresponding 13 C-labelled metabolite form with negative intensity (down). HT2, HT-2 toxin; T2, T-2 toxin.

    Article Snippet: Qualitative Screening Experiment Measurements of 12 C/13 C-toxin- and mock-treated samples were performed with an UltiMate 3000 HPLC system combined with an Exactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany).

    Techniques: Mass Spectrometry, Software

    LSI-MS summed full and inset mass spectra of delipified fresh tissue on plain glass slide spotted with 2,5-DHAP matrix in 50:50 ACN:water showing multiple charged protein ions using Orbitrap Exactive mass spectrometer. Rel. Abund. , relative abundance.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Laserspray Ionization, a New Method for Protein Analysis Directly from Tissue at Atmospheric Pressure with Ultrahigh Mass Resolution and Electron Transfer Dissociation *

    doi: 10.1074/mcp.M110.000760

    Figure Lengend Snippet: LSI-MS summed full and inset mass spectra of delipified fresh tissue on plain glass slide spotted with 2,5-DHAP matrix in 50:50 ACN:water showing multiple charged protein ions using Orbitrap Exactive mass spectrometer. Rel. Abund. , relative abundance.

    Article Snippet: Searches within 1-Da mass error were performed, but to consider a peptide as well matched, it had to be within < 5 ppm of the mass obtained from the Orbitrap Exactive.

    Techniques: Mass Spectrometry

    LSI mass spectra from aged mouse brain tissue washed with ethanol and spotted with 2,5-DHAP matrix in 50:50 ACN:water using an Orbitrap Exactive mass spectrometer. A , full mass spectrum with insets showing multiple charged protein and peptide ions. B , limited mass range between m/z 650 and 1000 is displayed with monoisotopic molecular weights of the various multiply charged ions presented. Rel. Abund. , relative abundance.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Laserspray Ionization, a New Method for Protein Analysis Directly from Tissue at Atmospheric Pressure with Ultrahigh Mass Resolution and Electron Transfer Dissociation *

    doi: 10.1074/mcp.M110.000760

    Figure Lengend Snippet: LSI mass spectra from aged mouse brain tissue washed with ethanol and spotted with 2,5-DHAP matrix in 50:50 ACN:water using an Orbitrap Exactive mass spectrometer. A , full mass spectrum with insets showing multiple charged protein and peptide ions. B , limited mass range between m/z 650 and 1000 is displayed with monoisotopic molecular weights of the various multiply charged ions presented. Rel. Abund. , relative abundance.

    Article Snippet: Searches within 1-Da mass error were performed, but to consider a peptide as well matched, it had to be within < 5 ppm of the mass obtained from the Orbitrap Exactive.

    Techniques: Mass Spectrometry

    Representative MS-MS spectra of significantly altered proteins obtained from Q-ExactivePlus Orbitrap mass spectrometer study. (A) MS-MS spectra of Plasminogen (PLG) protein. (B) MS-MS spectra of Vitronectin (VTN) protein. (C) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to HI. (D) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to POLY lesions. (E) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to CR individuals. (F) Validation of Plasminogen and Vitronectin using western blotting image Ponceau stained image of the blot after transfer.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Clinical Proteomics Profiling for Biomarker Identification Among Patients Suffering With Indian Post Kala Azar Dermal Leishmaniasis

    doi: 10.3389/fcimb.2020.00251

    Figure Lengend Snippet: Representative MS-MS spectra of significantly altered proteins obtained from Q-ExactivePlus Orbitrap mass spectrometer study. (A) MS-MS spectra of Plasminogen (PLG) protein. (B) MS-MS spectra of Vitronectin (VTN) protein. (C) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to HI. (D) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to POLY lesions. (E) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to CR individuals. (F) Validation of Plasminogen and Vitronectin using western blotting image Ponceau stained image of the blot after transfer.

    Article Snippet: Peptides were analyzed by electrospray ionization mass spectrometry using the Easy nano LC 1200 system [Thermo Scientific] coupled to a Q-Exactive Plus Orbitrap mass spectrometer [Thermo Scientific].

    Techniques: Tandem Mass Spectroscopy, Mass Spectrometry, Western Blot, Staining