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  • 99
    Thermo Fisher optimem medium
    DNA binding ability of fluorescent compounds was evaluated in <t>OptiMEM</t> by mixing a constant mass of DNA (1 μg) with different concentrations of cationic lipids corresponding to different CRs. DNA full complexation was highlighted by the absence of DNA migration into the agarose gel, at a given CR depending on the formulations tested.
    Optimem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem medium/product/Thermo Fisher
    Average 99 stars, based on 5142 article reviews
    Price from $9.99 to $1999.99
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    92
    Lonza optimem medium
    DNA binding ability of fluorescent compounds was evaluated in <t>OptiMEM</t> by mixing a constant mass of DNA (1 μg) with different concentrations of cationic lipids corresponding to different CRs. DNA full complexation was highlighted by the absence of DNA migration into the agarose gel, at a given CR depending on the formulations tested.
    Optimem Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem medium/product/Lonza
    Average 92 stars, based on 29 article reviews
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    92
    Becton Dickinson optimem medium
    DNA binding ability of fluorescent compounds was evaluated in <t>OptiMEM</t> by mixing a constant mass of DNA (1 μg) with different concentrations of cationic lipids corresponding to different CRs. DNA full complexation was highlighted by the absence of DNA migration into the agarose gel, at a given CR depending on the formulations tested.
    Optimem Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem medium/product/Becton Dickinson
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    optimem medium - by Bioz Stars, 2020-08
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    92
    Millipore optimem medium
    DNA binding ability of fluorescent compounds was evaluated in <t>OptiMEM</t> by mixing a constant mass of DNA (1 μg) with different concentrations of cationic lipids corresponding to different CRs. DNA full complexation was highlighted by the absence of DNA migration into the agarose gel, at a given CR depending on the formulations tested.
    Optimem Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem medium/product/Millipore
    Average 92 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    optimem medium - by Bioz Stars, 2020-08
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    99
    Thermo Fisher serum medium optimem
    Ultraviolet/visible (UV/vis) absorption spectra of LPEI suspended at varying concentrations in: (a) DI water, or (b) <t>OptiMEM</t> buffer, without siRNA, showed maximum peaks at 240 nm and 244 nm, respectively. (“LPEI concentration (≡ N/P ratio)”
    Serum Medium Optimem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serum medium optimem/product/Thermo Fisher
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    92
    Fisher Scientific optimem medium
    Ultraviolet/visible (UV/vis) absorption spectra of LPEI suspended at varying concentrations in: (a) DI water, or (b) <t>OptiMEM</t> buffer, without siRNA, showed maximum peaks at 240 nm and 244 nm, respectively. (“LPEI concentration (≡ N/P ratio)”
    Optimem Medium, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem medium/product/Fisher Scientific
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    90
    Roche optimem medium
    Ultraviolet/visible (UV/vis) absorption spectra of LPEI suspended at varying concentrations in: (a) DI water, or (b) <t>OptiMEM</t> buffer, without siRNA, showed maximum peaks at 240 nm and 244 nm, respectively. (“LPEI concentration (≡ N/P ratio)”
    Optimem Medium, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem medium/product/Roche
    Average 90 stars, based on 12 article reviews
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    86
    Thermo Fisher optimem glutamax medium
    Ultraviolet/visible (UV/vis) absorption spectra of LPEI suspended at varying concentrations in: (a) DI water, or (b) <t>OptiMEM</t> buffer, without siRNA, showed maximum peaks at 240 nm and 244 nm, respectively. (“LPEI concentration (≡ N/P ratio)”
    Optimem Glutamax Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem glutamax medium/product/Thermo Fisher
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    85
    Thermo Fisher optimem omem medium
    Ultraviolet/visible (UV/vis) absorption spectra of LPEI suspended at varying concentrations in: (a) DI water, or (b) <t>OptiMEM</t> buffer, without siRNA, showed maximum peaks at 240 nm and 244 nm, respectively. (“LPEI concentration (≡ N/P ratio)”
    Optimem Omem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem omem medium/product/Thermo Fisher
    Average 85 stars, based on 7 article reviews
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    91
    Thermo Fisher cacl2 optimem medium
    Ultraviolet/visible (UV/vis) absorption spectra of LPEI suspended at varying concentrations in: (a) DI water, or (b) <t>OptiMEM</t> buffer, without siRNA, showed maximum peaks at 240 nm and 244 nm, respectively. (“LPEI concentration (≡ N/P ratio)”
    Cacl2 Optimem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2 optimem medium/product/Thermo Fisher
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    85
    Thermo Fisher optimem complexing medium
    Ultraviolet/visible (UV/vis) absorption spectra of LPEI suspended at varying concentrations in: (a) DI water, or (b) <t>OptiMEM</t> buffer, without siRNA, showed maximum peaks at 240 nm and 244 nm, respectively. (“LPEI concentration (≡ N/P ratio)”
    Optimem Complexing Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    DNA binding ability of fluorescent compounds was evaluated in OptiMEM by mixing a constant mass of DNA (1 μg) with different concentrations of cationic lipids corresponding to different CRs. DNA full complexation was highlighted by the absence of DNA migration into the agarose gel, at a given CR depending on the formulations tested.

    Journal: International Journal of Molecular Sciences

    Article Title: Evaluation of New Fluorescent Lipophosphoramidates for Gene Transfer and Biodistribution Studies after Systemic Administration

    doi: 10.3390/ijms161125941

    Figure Lengend Snippet: DNA binding ability of fluorescent compounds was evaluated in OptiMEM by mixing a constant mass of DNA (1 μg) with different concentrations of cationic lipids corresponding to different CRs. DNA full complexation was highlighted by the absence of DNA migration into the agarose gel, at a given CR depending on the formulations tested.

    Article Snippet: Various lipoplexes characterized by several CRs (1 to 8) were prepared with a constant amount of DNA plasmid (0.25 µg) in OptiMEM medium (Gibco).

    Techniques: Binding Assay, Migration, Agarose Gel Electrophoresis

    Vesicular ATX stimulation of signalling is dependent of LPA receptors. Exosomes were added to serum-starved HEK293 cells that had been pre-treated with either EtOH or 10 μM Ki16425. His ATX T210A is a catalytically inactive mutant. (A) Phosphorylated (pERK) and ERK2 in lysates were detected by immunoblotting. (B) Phosphorylated (pAKT) and total AKT in lysates were detected by immunoblotting. Blots were quantified by using scanning densitometry analysis. (C) Exosomes induce Ca 2+ mobilisation in NIH3T3 cells in an LPA-like manner. Quiescent cells were seeded onto glass coverslips and loaded with Fura-2 AM, which was alternately excited at 340 nm and 380 nm wavelengths, and the Fura-2 emission at > 450 nm was sampled at 2 Hz. The y -axis represents the ratiometric Fura-2 emission, and is proportional to cytosolic Ca 2+ concentration. Cells were stimulated with exosomes followed by a threshold and a maximal dose of LPA. The cytosolic Ca 2+ concentration in four individual cells is shown. The arrows indicate the series of low-amplitude exosome-induced transient Ca 2+ elevations, which were not seen in untreated cells, or in cells that had been co-incubated with exosomes and Ki16425 ( n =40 cells imaged on four separate days). (D) HEK293 cells were transfected with His–ATX, His–ATX-RGE, His–ATX-LNV, His–ATX-RGE–LNV, His–ATX-H119A or His–ATX-ΔSMB and cultured in OptiMEM for 36 h to condition media. Exosomes were isolated by differential centrifugation and added to serum-starved NIH3T3 cells. Phosphorylated and total ERK were detected by immunoblotting of lysates. Bands were quantified by performing scanning densitometry analysis and normalised to levels of total ERK. The shown immunoblot is representative of three experiments, and quantification is mean±s.d. of three experiments. The immunoblots shown in A,B,D are from the same membranes. * P ≤0.05, ** P ≤0.01, *** P ≤0.0001 (Student's t -test). Control, OptiMEM without LPA or exosomes.

    Journal: Journal of Cell Science

    Article Title: Exosomes bind to autotaxin and act as a physiological delivery mechanism to stimulate LPA receptor signalling in cells

    doi: 10.1242/jcs.184424

    Figure Lengend Snippet: Vesicular ATX stimulation of signalling is dependent of LPA receptors. Exosomes were added to serum-starved HEK293 cells that had been pre-treated with either EtOH or 10 μM Ki16425. His ATX T210A is a catalytically inactive mutant. (A) Phosphorylated (pERK) and ERK2 in lysates were detected by immunoblotting. (B) Phosphorylated (pAKT) and total AKT in lysates were detected by immunoblotting. Blots were quantified by using scanning densitometry analysis. (C) Exosomes induce Ca 2+ mobilisation in NIH3T3 cells in an LPA-like manner. Quiescent cells were seeded onto glass coverslips and loaded with Fura-2 AM, which was alternately excited at 340 nm and 380 nm wavelengths, and the Fura-2 emission at > 450 nm was sampled at 2 Hz. The y -axis represents the ratiometric Fura-2 emission, and is proportional to cytosolic Ca 2+ concentration. Cells were stimulated with exosomes followed by a threshold and a maximal dose of LPA. The cytosolic Ca 2+ concentration in four individual cells is shown. The arrows indicate the series of low-amplitude exosome-induced transient Ca 2+ elevations, which were not seen in untreated cells, or in cells that had been co-incubated with exosomes and Ki16425 ( n =40 cells imaged on four separate days). (D) HEK293 cells were transfected with His–ATX, His–ATX-RGE, His–ATX-LNV, His–ATX-RGE–LNV, His–ATX-H119A or His–ATX-ΔSMB and cultured in OptiMEM for 36 h to condition media. Exosomes were isolated by differential centrifugation and added to serum-starved NIH3T3 cells. Phosphorylated and total ERK were detected by immunoblotting of lysates. Bands were quantified by performing scanning densitometry analysis and normalised to levels of total ERK. The shown immunoblot is representative of three experiments, and quantification is mean±s.d. of three experiments. The immunoblots shown in A,B,D are from the same membranes. * P ≤0.05, ** P ≤0.01, *** P ≤0.0001 (Student's t -test). Control, OptiMEM without LPA or exosomes.

    Article Snippet: Cells were cultured in OptiMEM serum-free medium (Invitrogen) at a density of 3×106 cells per ml for between 24 and 72 h to generate conditioned media.

    Techniques: Mutagenesis, Concentration Assay, Incubation, Transfection, Cell Culture, Isolation, Centrifugation, Western Blot

    ATX induces migration but not DNA synthesis in manner that is dependent on LPA receptors . (A) Exosomes isolated from media conditioned by either untransfected or His–ATX-transfected HEK293 cells or LPA were added to quiescent NIH3T3 cells that had been pre-treated with EtOH (white bars) or 10 μM Ki16425 (black bars) for 24 h. The cells were then pulsed with [ 3 H] thymidine for 6 h, fixed and lysed, and the radioactivity was determined. Results are mean±s.d. of three experiments performed in triplicate. (B) Exosomes±10 µM Ki16425 were added to cultures of serum-starved HEK293 cells that had been scratched to leave a clear region into which cells migrated in response to the addition of exosomes±Ki16425. Results are mean±s.d. of two experiments each performed in triplicate. x-axis in B,C indicates the percentage change in the area of the wound covered. (C) Exosomes±10 µM HA-130 were added to cultures of serum-starved HEK293 cells that had been scratched to leave a clear region into which cells migrated in response to the addition of exosomes±HA-130. Results are the mean±s.d. of two experiments each performed in triplicate. * P ≤0.05, ** P ≤0.01, *** P ≤0.0001; ns, not significant (Student's t -test). Control, OptiMEM without exosomes. Additional chemicals were added as indicated in B and C.

    Journal: Journal of Cell Science

    Article Title: Exosomes bind to autotaxin and act as a physiological delivery mechanism to stimulate LPA receptor signalling in cells

    doi: 10.1242/jcs.184424

    Figure Lengend Snippet: ATX induces migration but not DNA synthesis in manner that is dependent on LPA receptors . (A) Exosomes isolated from media conditioned by either untransfected or His–ATX-transfected HEK293 cells or LPA were added to quiescent NIH3T3 cells that had been pre-treated with EtOH (white bars) or 10 μM Ki16425 (black bars) for 24 h. The cells were then pulsed with [ 3 H] thymidine for 6 h, fixed and lysed, and the radioactivity was determined. Results are mean±s.d. of three experiments performed in triplicate. (B) Exosomes±10 µM Ki16425 were added to cultures of serum-starved HEK293 cells that had been scratched to leave a clear region into which cells migrated in response to the addition of exosomes±Ki16425. Results are mean±s.d. of two experiments each performed in triplicate. x-axis in B,C indicates the percentage change in the area of the wound covered. (C) Exosomes±10 µM HA-130 were added to cultures of serum-starved HEK293 cells that had been scratched to leave a clear region into which cells migrated in response to the addition of exosomes±HA-130. Results are the mean±s.d. of two experiments each performed in triplicate. * P ≤0.05, ** P ≤0.01, *** P ≤0.0001; ns, not significant (Student's t -test). Control, OptiMEM without exosomes. Additional chemicals were added as indicated in B and C.

    Article Snippet: Cells were cultured in OptiMEM serum-free medium (Invitrogen) at a density of 3×106 cells per ml for between 24 and 72 h to generate conditioned media.

    Techniques: Migration, DNA Synthesis, Isolation, Transfection, Radioactivity

    Ultraviolet/visible (UV/vis) absorption spectra of LPEI suspended at varying concentrations in: (a) DI water, or (b) OptiMEM buffer, without siRNA, showed maximum peaks at 240 nm and 244 nm, respectively. (“LPEI concentration (≡ N/P ratio)”

    Journal: Acta biomaterialia

    Article Title: Multilayer mediated forward and patterned siRNA transfection using linear-PEI at extended N/P ratios

    doi: 10.1016/j.actbio.2009.01.004

    Figure Lengend Snippet: Ultraviolet/visible (UV/vis) absorption spectra of LPEI suspended at varying concentrations in: (a) DI water, or (b) OptiMEM buffer, without siRNA, showed maximum peaks at 240 nm and 244 nm, respectively. (“LPEI concentration (≡ N/P ratio)”

    Article Snippet: Lipofectamine 2000 (LF2k) transfection reagent (1 mg ml−1 ), OptiMEM reduced serum medium (catalog no. 31985), Dulbecco’s Modified Eagle Medium (DMEM), penicillin, streptomycin, were purchased from Invitrogen (Carlsbad, CA).

    Techniques: Concentration Assay

    CMAH in melanoma cells. (A) RT-PCR analysis of CMAH expression in L6 (cluster 1), L34 (cluster 2), L4 (cluster 3). (B) RT-PCR analysis of deletion at 5′ of CMAH gene in L6 (cluster 1), L34 (cluster 2), L4 (cluster 3). Similar results were obtained for other melanoma cell lines according to the clustering. (C) HPTLC separation of gangliosides of L6 cells after pre-incubation in medium containing 10% FBS or in serum-reduced medium OptiMEM. Three replicate experiments were performed.

    Journal: BMC Cancer

    Article Title: Molecular subtyping of metastatic melanoma based on cell ganglioside metabolism profiles

    doi: 10.1186/1471-2407-14-560

    Figure Lengend Snippet: CMAH in melanoma cells. (A) RT-PCR analysis of CMAH expression in L6 (cluster 1), L34 (cluster 2), L4 (cluster 3). (B) RT-PCR analysis of deletion at 5′ of CMAH gene in L6 (cluster 1), L34 (cluster 2), L4 (cluster 3). Similar results were obtained for other melanoma cell lines according to the clustering. (C) HPTLC separation of gangliosides of L6 cells after pre-incubation in medium containing 10% FBS or in serum-reduced medium OptiMEM. Three replicate experiments were performed.

    Article Snippet: In order to assay the hypothesis that Neu5Gc-glicans could be incorporated from the culture medium and then employed for the synthesis of GM3, before [3-3 H]sphingosine labeling, melanoma L6 cells were pre-incubated in the reduced-serum medium OptiMEM (Life Technology, Carlsbad, CA, USA) for 5 days.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, High Performance Thin Layer Chromatography, Incubation