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  • 99
    Thermo Fisher optimem medium
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Optimem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher optimem reduced serum medium
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Optimem Reduced Serum Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher optimem i medium
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Optimem I Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 386 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher optimem gibco medium
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Optimem Gibco Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher optimem culture medium
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Optimem Culture Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher optimem transfection medium
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Optimem Transfection Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher optimem cell culture medium
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Optimem Cell Culture Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher optimem invitrogen medium
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Optimem Invitrogen Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher antibiotics free optimem medium
    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in <t>OptiMEM</t> medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.
    Antibiotics Free Optimem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in OptiMEM medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.

    Journal: Autophagy

    Article Title: Using LC3 to Monitor Autophagy Flux in the Retinal Pigment Epithelium

    doi:

    Figure Lengend Snippet: Representative micrograph showing GFP-labeled autophagosomes (arrows) 24 h following transient transfection of ARPE19 cells with GFP-LC3 plasmid DNA in OptiMEM medium. The bar marker is 20 μM. The blot is simply a representative examples of what one expect to see.

    Article Snippet: Preparation of the transfection solution is as follows: Per well, dilute 0.3 μg GFP-LC3 plasmid DNA in 25 μl OptiMEM medium (no serum/antibiotics) in a sterile polystyrene tube and dilute 0.8 μl Lipofectamine 2000 (Invitrogen, 11668027) in 25 μl OptiMEM (no serum/antibiotics) into a second sterile polystyrene tube and incubate for 5 min at room temperature.

    Techniques: Labeling, Transfection, Plasmid Preparation, Marker