operetta high content microscope Search Results


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  • 88
    PerkinElmer operetta high content microscope
    Microscopy‐based <t>High</t> <t>Content</t> <t>Screening</t> (HCS) of IFN regulatory factor 3 (IRF3) and NF‐κB p65 nuclear translocation. A, Overview of the microscopy‐based gene silencing screen. A549 cells plated in 96‐well plates are transduced with 5 independent lentivirus‐encoding short hairpin RNA (shRNA) per gene (1 shRNA per well) at a multiplicity of infection (MOI) of 10 for 4 days to silence expression of 60 nuclear transport factors. A control shRNA NT is included in each 96‐well plate. Cells are infected with Sendai virus (SeV) for 1, 3, 5, 8 or 10 hours before fixation, permeabilization, Hoechst nuclear labeling and antibody staining of IRF3 or NF‐κB p65 with Alexa Fluor 488 (green). Images of cells are captured in 9 pre‐determined fields for each well using an <t>Operetta</t> HCS <t>Microscope.</t> Images are processed using Harmony software to delimitate the nuclear region and measure the fluorescence intensity of IRF3 or NF‐κB p65 within the nucleus. For each 96‐well plate, a fluorescence cut‐off is set to allow automated discrimination of cells with (green) or without (red) IRF3 or NF‐κB p65 nuclear staining and to calculate the percentage of cells for each shRNA‐mediated gene knockdown. Scale bar is equal to 100 μM. B, Representative time course imaging performed with the control shRNA NT showing the nuclear translocation of IRF3 or NF‐κB p65 over a 10‐hour Sendai virus (SeV) infection (1 representative of 9 field images). Scale bar is equal to 100 μM. C, Graphic representation of the microscopy image‐based analysis showing an increase in the percentage of cells with positive nuclear staining for IRF3 or NF‐κB p65 culminating with ~75% of positive cells at 5 hours post‐infection followed by a decrease to ~30% of positive cells at 10 hours. D, Immunoblot analysis of total cell lysates, cytoplasmic and nuclear extracts of A549 cells infected with lentivirus‐encoding shRNA NT at a MOI of 10 for 3 days and infected with SeV for 0, 1, 3, 5, 8 and 10 hours prior to cell harvesting
    Operetta High Content Microscope, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta high content microscope/product/PerkinElmer
    Average 88 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    operetta high content microscope - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    93
    PerkinElmer operetta high content imaging microscope
    Microscopy‐based <t>High</t> <t>Content</t> <t>Screening</t> (HCS) of IFN regulatory factor 3 (IRF3) and NF‐κB p65 nuclear translocation. A, Overview of the microscopy‐based gene silencing screen. A549 cells plated in 96‐well plates are transduced with 5 independent lentivirus‐encoding short hairpin RNA (shRNA) per gene (1 shRNA per well) at a multiplicity of infection (MOI) of 10 for 4 days to silence expression of 60 nuclear transport factors. A control shRNA NT is included in each 96‐well plate. Cells are infected with Sendai virus (SeV) for 1, 3, 5, 8 or 10 hours before fixation, permeabilization, Hoechst nuclear labeling and antibody staining of IRF3 or NF‐κB p65 with Alexa Fluor 488 (green). Images of cells are captured in 9 pre‐determined fields for each well using an <t>Operetta</t> HCS <t>Microscope.</t> Images are processed using Harmony software to delimitate the nuclear region and measure the fluorescence intensity of IRF3 or NF‐κB p65 within the nucleus. For each 96‐well plate, a fluorescence cut‐off is set to allow automated discrimination of cells with (green) or without (red) IRF3 or NF‐κB p65 nuclear staining and to calculate the percentage of cells for each shRNA‐mediated gene knockdown. Scale bar is equal to 100 μM. B, Representative time course imaging performed with the control shRNA NT showing the nuclear translocation of IRF3 or NF‐κB p65 over a 10‐hour Sendai virus (SeV) infection (1 representative of 9 field images). Scale bar is equal to 100 μM. C, Graphic representation of the microscopy image‐based analysis showing an increase in the percentage of cells with positive nuclear staining for IRF3 or NF‐κB p65 culminating with ~75% of positive cells at 5 hours post‐infection followed by a decrease to ~30% of positive cells at 10 hours. D, Immunoblot analysis of total cell lysates, cytoplasmic and nuclear extracts of A549 cells infected with lentivirus‐encoding shRNA NT at a MOI of 10 for 3 days and infected with SeV for 0, 1, 3, 5, 8 and 10 hours prior to cell harvesting
    Operetta High Content Imaging Microscope, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta high content imaging microscope/product/PerkinElmer
    Average 93 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    operetta high content imaging microscope - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    88
    PerkinElmer high content microscopy operetta system
    Microscopy‐based <t>High</t> <t>Content</t> <t>Screening</t> (HCS) of IFN regulatory factor 3 (IRF3) and NF‐κB p65 nuclear translocation. A, Overview of the microscopy‐based gene silencing screen. A549 cells plated in 96‐well plates are transduced with 5 independent lentivirus‐encoding short hairpin RNA (shRNA) per gene (1 shRNA per well) at a multiplicity of infection (MOI) of 10 for 4 days to silence expression of 60 nuclear transport factors. A control shRNA NT is included in each 96‐well plate. Cells are infected with Sendai virus (SeV) for 1, 3, 5, 8 or 10 hours before fixation, permeabilization, Hoechst nuclear labeling and antibody staining of IRF3 or NF‐κB p65 with Alexa Fluor 488 (green). Images of cells are captured in 9 pre‐determined fields for each well using an <t>Operetta</t> HCS <t>Microscope.</t> Images are processed using Harmony software to delimitate the nuclear region and measure the fluorescence intensity of IRF3 or NF‐κB p65 within the nucleus. For each 96‐well plate, a fluorescence cut‐off is set to allow automated discrimination of cells with (green) or without (red) IRF3 or NF‐κB p65 nuclear staining and to calculate the percentage of cells for each shRNA‐mediated gene knockdown. Scale bar is equal to 100 μM. B, Representative time course imaging performed with the control shRNA NT showing the nuclear translocation of IRF3 or NF‐κB p65 over a 10‐hour Sendai virus (SeV) infection (1 representative of 9 field images). Scale bar is equal to 100 μM. C, Graphic representation of the microscopy image‐based analysis showing an increase in the percentage of cells with positive nuclear staining for IRF3 or NF‐κB p65 culminating with ~75% of positive cells at 5 hours post‐infection followed by a decrease to ~30% of positive cells at 10 hours. D, Immunoblot analysis of total cell lysates, cytoplasmic and nuclear extracts of A549 cells infected with lentivirus‐encoding shRNA NT at a MOI of 10 for 3 days and infected with SeV for 0, 1, 3, 5, 8 and 10 hours prior to cell harvesting
    High Content Microscopy Operetta System, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high content microscopy operetta system/product/PerkinElmer
    Average 88 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    high content microscopy operetta system - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    91
    PerkinElmer operetta automated high content screening microscope
    Microscopy‐based <t>High</t> <t>Content</t> <t>Screening</t> (HCS) of IFN regulatory factor 3 (IRF3) and NF‐κB p65 nuclear translocation. A, Overview of the microscopy‐based gene silencing screen. A549 cells plated in 96‐well plates are transduced with 5 independent lentivirus‐encoding short hairpin RNA (shRNA) per gene (1 shRNA per well) at a multiplicity of infection (MOI) of 10 for 4 days to silence expression of 60 nuclear transport factors. A control shRNA NT is included in each 96‐well plate. Cells are infected with Sendai virus (SeV) for 1, 3, 5, 8 or 10 hours before fixation, permeabilization, Hoechst nuclear labeling and antibody staining of IRF3 or NF‐κB p65 with Alexa Fluor 488 (green). Images of cells are captured in 9 pre‐determined fields for each well using an <t>Operetta</t> HCS <t>Microscope.</t> Images are processed using Harmony software to delimitate the nuclear region and measure the fluorescence intensity of IRF3 or NF‐κB p65 within the nucleus. For each 96‐well plate, a fluorescence cut‐off is set to allow automated discrimination of cells with (green) or without (red) IRF3 or NF‐κB p65 nuclear staining and to calculate the percentage of cells for each shRNA‐mediated gene knockdown. Scale bar is equal to 100 μM. B, Representative time course imaging performed with the control shRNA NT showing the nuclear translocation of IRF3 or NF‐κB p65 over a 10‐hour Sendai virus (SeV) infection (1 representative of 9 field images). Scale bar is equal to 100 μM. C, Graphic representation of the microscopy image‐based analysis showing an increase in the percentage of cells with positive nuclear staining for IRF3 or NF‐κB p65 culminating with ~75% of positive cells at 5 hours post‐infection followed by a decrease to ~30% of positive cells at 10 hours. D, Immunoblot analysis of total cell lysates, cytoplasmic and nuclear extracts of A549 cells infected with lentivirus‐encoding shRNA NT at a MOI of 10 for 3 days and infected with SeV for 0, 1, 3, 5, 8 and 10 hours prior to cell harvesting
    Operetta Automated High Content Screening Microscope, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta automated high content screening microscope/product/PerkinElmer
    Average 91 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    operetta automated high content screening microscope - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    93
    PerkinElmer operetta high content imaging fluorescence microscope
    Microscopy‐based <t>High</t> <t>Content</t> <t>Screening</t> (HCS) of IFN regulatory factor 3 (IRF3) and NF‐κB p65 nuclear translocation. A, Overview of the microscopy‐based gene silencing screen. A549 cells plated in 96‐well plates are transduced with 5 independent lentivirus‐encoding short hairpin RNA (shRNA) per gene (1 shRNA per well) at a multiplicity of infection (MOI) of 10 for 4 days to silence expression of 60 nuclear transport factors. A control shRNA NT is included in each 96‐well plate. Cells are infected with Sendai virus (SeV) for 1, 3, 5, 8 or 10 hours before fixation, permeabilization, Hoechst nuclear labeling and antibody staining of IRF3 or NF‐κB p65 with Alexa Fluor 488 (green). Images of cells are captured in 9 pre‐determined fields for each well using an <t>Operetta</t> HCS <t>Microscope.</t> Images are processed using Harmony software to delimitate the nuclear region and measure the fluorescence intensity of IRF3 or NF‐κB p65 within the nucleus. For each 96‐well plate, a fluorescence cut‐off is set to allow automated discrimination of cells with (green) or without (red) IRF3 or NF‐κB p65 nuclear staining and to calculate the percentage of cells for each shRNA‐mediated gene knockdown. Scale bar is equal to 100 μM. B, Representative time course imaging performed with the control shRNA NT showing the nuclear translocation of IRF3 or NF‐κB p65 over a 10‐hour Sendai virus (SeV) infection (1 representative of 9 field images). Scale bar is equal to 100 μM. C, Graphic representation of the microscopy image‐based analysis showing an increase in the percentage of cells with positive nuclear staining for IRF3 or NF‐κB p65 culminating with ~75% of positive cells at 5 hours post‐infection followed by a decrease to ~30% of positive cells at 10 hours. D, Immunoblot analysis of total cell lysates, cytoplasmic and nuclear extracts of A549 cells infected with lentivirus‐encoding shRNA NT at a MOI of 10 for 3 days and infected with SeV for 0, 1, 3, 5, 8 and 10 hours prior to cell harvesting
    Operetta High Content Imaging Fluorescence Microscope, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta high content imaging fluorescence microscope/product/PerkinElmer
    Average 93 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    operetta high content imaging fluorescence microscope - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    90
    Bio-Rad operetta high content microscope
    Microscopy‐based <t>High</t> <t>Content</t> <t>Screening</t> (HCS) of IFN regulatory factor 3 (IRF3) and NF‐κB p65 nuclear translocation. A, Overview of the microscopy‐based gene silencing screen. A549 cells plated in 96‐well plates are transduced with 5 independent lentivirus‐encoding short hairpin RNA (shRNA) per gene (1 shRNA per well) at a multiplicity of infection (MOI) of 10 for 4 days to silence expression of 60 nuclear transport factors. A control shRNA NT is included in each 96‐well plate. Cells are infected with Sendai virus (SeV) for 1, 3, 5, 8 or 10 hours before fixation, permeabilization, Hoechst nuclear labeling and antibody staining of IRF3 or NF‐κB p65 with Alexa Fluor 488 (green). Images of cells are captured in 9 pre‐determined fields for each well using an <t>Operetta</t> HCS <t>Microscope.</t> Images are processed using Harmony software to delimitate the nuclear region and measure the fluorescence intensity of IRF3 or NF‐κB p65 within the nucleus. For each 96‐well plate, a fluorescence cut‐off is set to allow automated discrimination of cells with (green) or without (red) IRF3 or NF‐κB p65 nuclear staining and to calculate the percentage of cells for each shRNA‐mediated gene knockdown. Scale bar is equal to 100 μM. B, Representative time course imaging performed with the control shRNA NT showing the nuclear translocation of IRF3 or NF‐κB p65 over a 10‐hour Sendai virus (SeV) infection (1 representative of 9 field images). Scale bar is equal to 100 μM. C, Graphic representation of the microscopy image‐based analysis showing an increase in the percentage of cells with positive nuclear staining for IRF3 or NF‐κB p65 culminating with ~75% of positive cells at 5 hours post‐infection followed by a decrease to ~30% of positive cells at 10 hours. D, Immunoblot analysis of total cell lysates, cytoplasmic and nuclear extracts of A549 cells infected with lentivirus‐encoding shRNA NT at a MOI of 10 for 3 days and infected with SeV for 0, 1, 3, 5, 8 and 10 hours prior to cell harvesting
    Operetta High Content Microscope, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta high content microscope/product/Bio-Rad
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    operetta high content microscope - by Bioz Stars, 2020-08
    90/100 stars
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    84
    PerkinElmer operetta cls high content screening microscope
    Microscopy‐based <t>High</t> <t>Content</t> <t>Screening</t> (HCS) of IFN regulatory factor 3 (IRF3) and NF‐κB p65 nuclear translocation. A, Overview of the microscopy‐based gene silencing screen. A549 cells plated in 96‐well plates are transduced with 5 independent lentivirus‐encoding short hairpin RNA (shRNA) per gene (1 shRNA per well) at a multiplicity of infection (MOI) of 10 for 4 days to silence expression of 60 nuclear transport factors. A control shRNA NT is included in each 96‐well plate. Cells are infected with Sendai virus (SeV) for 1, 3, 5, 8 or 10 hours before fixation, permeabilization, Hoechst nuclear labeling and antibody staining of IRF3 or NF‐κB p65 with Alexa Fluor 488 (green). Images of cells are captured in 9 pre‐determined fields for each well using an <t>Operetta</t> HCS <t>Microscope.</t> Images are processed using Harmony software to delimitate the nuclear region and measure the fluorescence intensity of IRF3 or NF‐κB p65 within the nucleus. For each 96‐well plate, a fluorescence cut‐off is set to allow automated discrimination of cells with (green) or without (red) IRF3 or NF‐κB p65 nuclear staining and to calculate the percentage of cells for each shRNA‐mediated gene knockdown. Scale bar is equal to 100 μM. B, Representative time course imaging performed with the control shRNA NT showing the nuclear translocation of IRF3 or NF‐κB p65 over a 10‐hour Sendai virus (SeV) infection (1 representative of 9 field images). Scale bar is equal to 100 μM. C, Graphic representation of the microscopy image‐based analysis showing an increase in the percentage of cells with positive nuclear staining for IRF3 or NF‐κB p65 culminating with ~75% of positive cells at 5 hours post‐infection followed by a decrease to ~30% of positive cells at 10 hours. D, Immunoblot analysis of total cell lysates, cytoplasmic and nuclear extracts of A549 cells infected with lentivirus‐encoding shRNA NT at a MOI of 10 for 3 days and infected with SeV for 0, 1, 3, 5, 8 and 10 hours prior to cell harvesting
    Operetta Cls High Content Screening Microscope, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta cls high content screening microscope/product/PerkinElmer
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    operetta cls high content screening microscope - by Bioz Stars, 2020-08
    84/100 stars
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    93
    PerkinElmer operetta automated high content screening fluorescence microscope
    Microscopy‐based <t>High</t> <t>Content</t> <t>Screening</t> (HCS) of IFN regulatory factor 3 (IRF3) and NF‐κB p65 nuclear translocation. A, Overview of the microscopy‐based gene silencing screen. A549 cells plated in 96‐well plates are transduced with 5 independent lentivirus‐encoding short hairpin RNA (shRNA) per gene (1 shRNA per well) at a multiplicity of infection (MOI) of 10 for 4 days to silence expression of 60 nuclear transport factors. A control shRNA NT is included in each 96‐well plate. Cells are infected with Sendai virus (SeV) for 1, 3, 5, 8 or 10 hours before fixation, permeabilization, Hoechst nuclear labeling and antibody staining of IRF3 or NF‐κB p65 with Alexa Fluor 488 (green). Images of cells are captured in 9 pre‐determined fields for each well using an <t>Operetta</t> HCS <t>Microscope.</t> Images are processed using Harmony software to delimitate the nuclear region and measure the fluorescence intensity of IRF3 or NF‐κB p65 within the nucleus. For each 96‐well plate, a fluorescence cut‐off is set to allow automated discrimination of cells with (green) or without (red) IRF3 or NF‐κB p65 nuclear staining and to calculate the percentage of cells for each shRNA‐mediated gene knockdown. Scale bar is equal to 100 μM. B, Representative time course imaging performed with the control shRNA NT showing the nuclear translocation of IRF3 or NF‐κB p65 over a 10‐hour Sendai virus (SeV) infection (1 representative of 9 field images). Scale bar is equal to 100 μM. C, Graphic representation of the microscopy image‐based analysis showing an increase in the percentage of cells with positive nuclear staining for IRF3 or NF‐κB p65 culminating with ~75% of positive cells at 5 hours post‐infection followed by a decrease to ~30% of positive cells at 10 hours. D, Immunoblot analysis of total cell lysates, cytoplasmic and nuclear extracts of A549 cells infected with lentivirus‐encoding shRNA NT at a MOI of 10 for 3 days and infected with SeV for 0, 1, 3, 5, 8 and 10 hours prior to cell harvesting
    Operetta Automated High Content Screening Fluorescence Microscope, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta automated high content screening fluorescence microscope/product/PerkinElmer
    Average 93 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    operetta automated high content screening fluorescence microscope - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    PerkinElmer operetta high content imaging system
    ADAP1 restrains the serum-induced increase in cell size of cultured cardiomyocytes. ( A ) Western blot detection of adenovirus-mediated 3xFLAG-hADAP1 overexpression (MOI of 50) in rat neonatal ventricular cardiomyocytes (RNVC) cultured for 72 h post-infection. ( B ) RNVC were infected with either β-Gal- (negative control) or ADAP1-overexpressing adenovirus and were cultured for 72 h in the absence (0%) or presence (10%) of serum. Representative images of α-Actinin-immunostained RNVC (left) and corresponding segmented images were acquired using the <t>Operetta</t> <t>High-Content</t> <t>Imaging</t> <t>System</t> (Perkin Elmer). The scale bar represents 50 µm. ( C ) The histogram represents the cell surface areas of RNVC overexpressing either β-Gal or ADAP1 and cultured for 72 h with increasing concentrations of serum ( n = 3 independent experiments). * P
    Operetta High Content Imaging System, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 1519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta high content imaging system/product/PerkinElmer
    Average 92 stars, based on 1519 article reviews
    Price from $9.99 to $1999.99
    operetta high content imaging system - by Bioz Stars, 2020-08
    92/100 stars
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    91
    PerkinElmer operetta high content screening platform
    ADAP1 restrains the serum-induced increase in cell size of cultured cardiomyocytes. ( A ) Western blot detection of adenovirus-mediated 3xFLAG-hADAP1 overexpression (MOI of 50) in rat neonatal ventricular cardiomyocytes (RNVC) cultured for 72 h post-infection. ( B ) RNVC were infected with either β-Gal- (negative control) or ADAP1-overexpressing adenovirus and were cultured for 72 h in the absence (0%) or presence (10%) of serum. Representative images of α-Actinin-immunostained RNVC (left) and corresponding segmented images were acquired using the <t>Operetta</t> <t>High-Content</t> <t>Imaging</t> <t>System</t> (Perkin Elmer). The scale bar represents 50 µm. ( C ) The histogram represents the cell surface areas of RNVC overexpressing either β-Gal or ADAP1 and cultured for 72 h with increasing concentrations of serum ( n = 3 independent experiments). * P
    Operetta High Content Screening Platform, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta high content screening platform/product/PerkinElmer
    Average 91 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    operetta high content screening platform - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    88
    PerkinElmer operetta high content imager
    ADAP1 restrains the serum-induced increase in cell size of cultured cardiomyocytes. ( A ) Western blot detection of adenovirus-mediated 3xFLAG-hADAP1 overexpression (MOI of 50) in rat neonatal ventricular cardiomyocytes (RNVC) cultured for 72 h post-infection. ( B ) RNVC were infected with either β-Gal- (negative control) or ADAP1-overexpressing adenovirus and were cultured for 72 h in the absence (0%) or presence (10%) of serum. Representative images of α-Actinin-immunostained RNVC (left) and corresponding segmented images were acquired using the <t>Operetta</t> <t>High-Content</t> <t>Imaging</t> <t>System</t> (Perkin Elmer). The scale bar represents 50 µm. ( C ) The histogram represents the cell surface areas of RNVC overexpressing either β-Gal or ADAP1 and cultured for 72 h with increasing concentrations of serum ( n = 3 independent experiments). * P
    Operetta High Content Imager, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta high content imager/product/PerkinElmer
    Average 88 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    operetta high content imager - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    84
    PerkinElmer content fluorescent microscope operetta
    ADAP1 restrains the serum-induced increase in cell size of cultured cardiomyocytes. ( A ) Western blot detection of adenovirus-mediated 3xFLAG-hADAP1 overexpression (MOI of 50) in rat neonatal ventricular cardiomyocytes (RNVC) cultured for 72 h post-infection. ( B ) RNVC were infected with either β-Gal- (negative control) or ADAP1-overexpressing adenovirus and were cultured for 72 h in the absence (0%) or presence (10%) of serum. Representative images of α-Actinin-immunostained RNVC (left) and corresponding segmented images were acquired using the <t>Operetta</t> <t>High-Content</t> <t>Imaging</t> <t>System</t> (Perkin Elmer). The scale bar represents 50 µm. ( C ) The histogram represents the cell surface areas of RNVC overexpressing either β-Gal or ADAP1 and cultured for 72 h with increasing concentrations of serum ( n = 3 independent experiments). * P
    Content Fluorescent Microscope Operetta, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/content fluorescent microscope operetta/product/PerkinElmer
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    content fluorescent microscope operetta - by Bioz Stars, 2020-08
    84/100 stars
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    91
    PerkinElmer operetta cls high content analysis system
    ADAP1 restrains the serum-induced increase in cell size of cultured cardiomyocytes. ( A ) Western blot detection of adenovirus-mediated 3xFLAG-hADAP1 overexpression (MOI of 50) in rat neonatal ventricular cardiomyocytes (RNVC) cultured for 72 h post-infection. ( B ) RNVC were infected with either β-Gal- (negative control) or ADAP1-overexpressing adenovirus and were cultured for 72 h in the absence (0%) or presence (10%) of serum. Representative images of α-Actinin-immunostained RNVC (left) and corresponding segmented images were acquired using the <t>Operetta</t> <t>High-Content</t> <t>Imaging</t> <t>System</t> (Perkin Elmer). The scale bar represents 50 µm. ( C ) The histogram represents the cell surface areas of RNVC overexpressing either β-Gal or ADAP1 and cultured for 72 h with increasing concentrations of serum ( n = 3 independent experiments). * P
    Operetta Cls High Content Analysis System, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta cls high content analysis system/product/PerkinElmer
    Average 91 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    operetta cls high content analysis system - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    88
    PerkinElmer operetta high content analysis system
    Screening of substances with apoptotic effects in the natural product library. (a) MH7A cells were treated with the indicated concentrations of natural product extracts for 24 h. The cell viability was determined by using MTT assay. Compounds GLF, MSK, BCB, GGL, CML, and EJT, corresponding to Glehnia littoralis Fr. Schm ., Magnolia sieboldii K. Koch ., Boswellia carterii Birdw ., Gomphrena globose L ., Chelidonium majus L., and Eupatorium japonicum Thunb. (b) MH7A cells were treated with the indicated concentration of EJT extract for 12 h and then stained with Annexin V 488 (green) and Hoechst 33342 (blue) at 25°C (room temperature) for 30 min. The staining intensity of Annexin V in the membrane was observed by confocal laser scanning microscope (magnification 400×). (c) Annexin V intensity in the cell membrane region was analyzed by using the <t>Operetta</t> <t>high-content</t> imaging <t>system.</t>
    Operetta High Content Analysis System, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta high content analysis system/product/PerkinElmer
    Average 88 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    operetta high content analysis system - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    84
    PerkinElmer content fluorescence microscope operetta
    Screening of substances with apoptotic effects in the natural product library. (a) MH7A cells were treated with the indicated concentrations of natural product extracts for 24 h. The cell viability was determined by using MTT assay. Compounds GLF, MSK, BCB, GGL, CML, and EJT, corresponding to Glehnia littoralis Fr. Schm ., Magnolia sieboldii K. Koch ., Boswellia carterii Birdw ., Gomphrena globose L ., Chelidonium majus L., and Eupatorium japonicum Thunb. (b) MH7A cells were treated with the indicated concentration of EJT extract for 12 h and then stained with Annexin V 488 (green) and Hoechst 33342 (blue) at 25°C (room temperature) for 30 min. The staining intensity of Annexin V in the membrane was observed by confocal laser scanning microscope (magnification 400×). (c) Annexin V intensity in the cell membrane region was analyzed by using the <t>Operetta</t> <t>high-content</t> imaging <t>system.</t>
    Content Fluorescence Microscope Operetta, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/content fluorescence microscope operetta/product/PerkinElmer
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    content fluorescence microscope operetta - by Bioz Stars, 2020-08
    84/100 stars
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    Microscopy‐based High Content Screening (HCS) of IFN regulatory factor 3 (IRF3) and NF‐κB p65 nuclear translocation. A, Overview of the microscopy‐based gene silencing screen. A549 cells plated in 96‐well plates are transduced with 5 independent lentivirus‐encoding short hairpin RNA (shRNA) per gene (1 shRNA per well) at a multiplicity of infection (MOI) of 10 for 4 days to silence expression of 60 nuclear transport factors. A control shRNA NT is included in each 96‐well plate. Cells are infected with Sendai virus (SeV) for 1, 3, 5, 8 or 10 hours before fixation, permeabilization, Hoechst nuclear labeling and antibody staining of IRF3 or NF‐κB p65 with Alexa Fluor 488 (green). Images of cells are captured in 9 pre‐determined fields for each well using an Operetta HCS Microscope. Images are processed using Harmony software to delimitate the nuclear region and measure the fluorescence intensity of IRF3 or NF‐κB p65 within the nucleus. For each 96‐well plate, a fluorescence cut‐off is set to allow automated discrimination of cells with (green) or without (red) IRF3 or NF‐κB p65 nuclear staining and to calculate the percentage of cells for each shRNA‐mediated gene knockdown. Scale bar is equal to 100 μM. B, Representative time course imaging performed with the control shRNA NT showing the nuclear translocation of IRF3 or NF‐κB p65 over a 10‐hour Sendai virus (SeV) infection (1 representative of 9 field images). Scale bar is equal to 100 μM. C, Graphic representation of the microscopy image‐based analysis showing an increase in the percentage of cells with positive nuclear staining for IRF3 or NF‐κB p65 culminating with ~75% of positive cells at 5 hours post‐infection followed by a decrease to ~30% of positive cells at 10 hours. D, Immunoblot analysis of total cell lysates, cytoplasmic and nuclear extracts of A549 cells infected with lentivirus‐encoding shRNA NT at a MOI of 10 for 3 days and infected with SeV for 0, 1, 3, 5, 8 and 10 hours prior to cell harvesting

    Journal: Traffic (Copenhagen, Denmark)

    Article Title: Importin β1 targeting by hepatitis C virus NS3/ 4A protein restricts IRF3 and NF‐κB signaling of IFNB1 antiviral response. Importin β1 targeting by hepatitis C virus NS3/4A protein restricts IRF3 and NF‐κB signaling of IFNB1 antiviral response

    doi: 10.1111/tra.12480

    Figure Lengend Snippet: Microscopy‐based High Content Screening (HCS) of IFN regulatory factor 3 (IRF3) and NF‐κB p65 nuclear translocation. A, Overview of the microscopy‐based gene silencing screen. A549 cells plated in 96‐well plates are transduced with 5 independent lentivirus‐encoding short hairpin RNA (shRNA) per gene (1 shRNA per well) at a multiplicity of infection (MOI) of 10 for 4 days to silence expression of 60 nuclear transport factors. A control shRNA NT is included in each 96‐well plate. Cells are infected with Sendai virus (SeV) for 1, 3, 5, 8 or 10 hours before fixation, permeabilization, Hoechst nuclear labeling and antibody staining of IRF3 or NF‐κB p65 with Alexa Fluor 488 (green). Images of cells are captured in 9 pre‐determined fields for each well using an Operetta HCS Microscope. Images are processed using Harmony software to delimitate the nuclear region and measure the fluorescence intensity of IRF3 or NF‐κB p65 within the nucleus. For each 96‐well plate, a fluorescence cut‐off is set to allow automated discrimination of cells with (green) or without (red) IRF3 or NF‐κB p65 nuclear staining and to calculate the percentage of cells for each shRNA‐mediated gene knockdown. Scale bar is equal to 100 μM. B, Representative time course imaging performed with the control shRNA NT showing the nuclear translocation of IRF3 or NF‐κB p65 over a 10‐hour Sendai virus (SeV) infection (1 representative of 9 field images). Scale bar is equal to 100 μM. C, Graphic representation of the microscopy image‐based analysis showing an increase in the percentage of cells with positive nuclear staining for IRF3 or NF‐κB p65 culminating with ~75% of positive cells at 5 hours post‐infection followed by a decrease to ~30% of positive cells at 10 hours. D, Immunoblot analysis of total cell lysates, cytoplasmic and nuclear extracts of A549 cells infected with lentivirus‐encoding shRNA NT at a MOI of 10 for 3 days and infected with SeV for 0, 1, 3, 5, 8 and 10 hours prior to cell harvesting

    Article Snippet: Images of cells were captured in 9 predetermined fields for each well (Operetta High Content Screening Microscope; Perkin Elmer) and images were processed using Harmony (Perkin Elmer).

    Techniques: Microscopy, High Content Screening, Translocation Assay, Transduction, shRNA, Infection, Expressing, Labeling, Staining, Software, Fluorescence, Imaging, Cell Harvesting

    ADAP1 restrains the serum-induced increase in cell size of cultured cardiomyocytes. ( A ) Western blot detection of adenovirus-mediated 3xFLAG-hADAP1 overexpression (MOI of 50) in rat neonatal ventricular cardiomyocytes (RNVC) cultured for 72 h post-infection. ( B ) RNVC were infected with either β-Gal- (negative control) or ADAP1-overexpressing adenovirus and were cultured for 72 h in the absence (0%) or presence (10%) of serum. Representative images of α-Actinin-immunostained RNVC (left) and corresponding segmented images were acquired using the Operetta High-Content Imaging System (Perkin Elmer). The scale bar represents 50 µm. ( C ) The histogram represents the cell surface areas of RNVC overexpressing either β-Gal or ADAP1 and cultured for 72 h with increasing concentrations of serum ( n = 3 independent experiments). * P

    Journal: Scientific Reports

    Article Title: ADAP1 limits neonatal cardiomyocyte hypertrophy by reducing integrin cell surface expression

    doi: 10.1038/s41598-018-31784-w

    Figure Lengend Snippet: ADAP1 restrains the serum-induced increase in cell size of cultured cardiomyocytes. ( A ) Western blot detection of adenovirus-mediated 3xFLAG-hADAP1 overexpression (MOI of 50) in rat neonatal ventricular cardiomyocytes (RNVC) cultured for 72 h post-infection. ( B ) RNVC were infected with either β-Gal- (negative control) or ADAP1-overexpressing adenovirus and were cultured for 72 h in the absence (0%) or presence (10%) of serum. Representative images of α-Actinin-immunostained RNVC (left) and corresponding segmented images were acquired using the Operetta High-Content Imaging System (Perkin Elmer). The scale bar represents 50 µm. ( C ) The histogram represents the cell surface areas of RNVC overexpressing either β-Gal or ADAP1 and cultured for 72 h with increasing concentrations of serum ( n = 3 independent experiments). * P

    Article Snippet: Images were acquired and analyzed using the Operetta High-Content Imaging System (Perkin Elmer) with a 20X objective.

    Techniques: Cell Culture, Western Blot, Over Expression, Infection, Negative Control, Imaging

    ADAP1 relocalizes cytoskeletal α-Actinin. ( A ) Representative confocal images (Olympus FluoView FV1000 microscope) of α-Actinin-immunostained rat neonatal ventricular cardiomyocytes (RNVC) infected with a β-Gal- (negative control), ADAP1-, or Mek1ca-overexpressing adenovirus, individually or in combination as indicated, and cultured for 72 h post-infection. Arrows point to α-Actinin dense puncta. The scale bar represents 12 µm. ( B ) Number of α-Actinin puncta per cell measured with the Operetta High-Content Imaging System (Perkin Elmer) using the same experimental conditions as in A ( n = 4 independent experiments). **** P

    Journal: Scientific Reports

    Article Title: ADAP1 limits neonatal cardiomyocyte hypertrophy by reducing integrin cell surface expression

    doi: 10.1038/s41598-018-31784-w

    Figure Lengend Snippet: ADAP1 relocalizes cytoskeletal α-Actinin. ( A ) Representative confocal images (Olympus FluoView FV1000 microscope) of α-Actinin-immunostained rat neonatal ventricular cardiomyocytes (RNVC) infected with a β-Gal- (negative control), ADAP1-, or Mek1ca-overexpressing adenovirus, individually or in combination as indicated, and cultured for 72 h post-infection. Arrows point to α-Actinin dense puncta. The scale bar represents 12 µm. ( B ) Number of α-Actinin puncta per cell measured with the Operetta High-Content Imaging System (Perkin Elmer) using the same experimental conditions as in A ( n = 4 independent experiments). **** P

    Article Snippet: Images were acquired and analyzed using the Operetta High-Content Imaging System (Perkin Elmer) with a 20X objective.

    Techniques: Microscopy, Infection, Negative Control, Cell Culture, Imaging

    Screening of substances with apoptotic effects in the natural product library. (a) MH7A cells were treated with the indicated concentrations of natural product extracts for 24 h. The cell viability was determined by using MTT assay. Compounds GLF, MSK, BCB, GGL, CML, and EJT, corresponding to Glehnia littoralis Fr. Schm ., Magnolia sieboldii K. Koch ., Boswellia carterii Birdw ., Gomphrena globose L ., Chelidonium majus L., and Eupatorium japonicum Thunb. (b) MH7A cells were treated with the indicated concentration of EJT extract for 12 h and then stained with Annexin V 488 (green) and Hoechst 33342 (blue) at 25°C (room temperature) for 30 min. The staining intensity of Annexin V in the membrane was observed by confocal laser scanning microscope (magnification 400×). (c) Annexin V intensity in the cell membrane region was analyzed by using the Operetta high-content imaging system.

    Journal: BioMed Research International

    Article Title: Apoptotic and Anti-Inflammatory Effects of Eupatorium japonicum Thunb. in Rheumatoid Arthritis Fibroblast-Like Synoviocytes

    doi: 10.1155/2018/1383697

    Figure Lengend Snippet: Screening of substances with apoptotic effects in the natural product library. (a) MH7A cells were treated with the indicated concentrations of natural product extracts for 24 h. The cell viability was determined by using MTT assay. Compounds GLF, MSK, BCB, GGL, CML, and EJT, corresponding to Glehnia littoralis Fr. Schm ., Magnolia sieboldii K. Koch ., Boswellia carterii Birdw ., Gomphrena globose L ., Chelidonium majus L., and Eupatorium japonicum Thunb. (b) MH7A cells were treated with the indicated concentration of EJT extract for 12 h and then stained with Annexin V 488 (green) and Hoechst 33342 (blue) at 25°C (room temperature) for 30 min. The staining intensity of Annexin V in the membrane was observed by confocal laser scanning microscope (magnification 400×). (c) Annexin V intensity in the cell membrane region was analyzed by using the Operetta high-content imaging system.

    Article Snippet: Annexin V intensity in the cell membrane region was analyzed by using the Operetta high-content analysis system (Perkin Elmer, MA).

    Techniques: MTT Assay, Concentration Assay, Staining, Laser-Scanning Microscopy, Imaging