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    New England Biolabs onetaqquick-load master mix with standard buffer
    Onetaqquick Load Master Mix With Standard Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaqquick-load master mix with standard buffer/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaqquick-load master mix with standard buffer - by Bioz Stars, 2023-09
    96/100 stars
      Buy from Supplier

    86
    New England Biolabs onetaq 2x mastermix
    Onetaq 2x Mastermix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq 2x mastermix/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq 2x mastermix - by Bioz Stars, 2023-09
    86/100 stars
      Buy from Supplier

    86
    New England Biolabs onetaq hotstart 2x mastermix
    An overview of the MRTK cloning process, parts within the toolkit, origin compatibility, origin copy number and antimicrobial susceptibility for each strain. A) Cloning plasmids (C plasmids) are used as starting material with BsaI restriction enzyme and NEBridge <t>MasterMix</t> to clone the product plasmid (P plasmid). P plasmids can then be transformed into the desired SynCom strain of interest with a time of ∼2.5 hours from plasmid to plate. B) Overview of all 45 parts included in the MRTK and their 4 bp golden gate overhang sequences. C) A table showing the compatibility and copy number of the origins of the MRTK for each strain as well as their antimicrobial susceptibility. Green boxes indicate that the origin is replicated in the strain and that the strain is susceptible to the antimicrobial agent. Red boxes indicate that the origin is not replicated in the strain and that the strain will grow on that antimicrobial agent.
    Onetaq Hotstart 2x Mastermix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq hotstart 2x mastermix/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq hotstart 2x mastermix - by Bioz Stars, 2023-09
    86/100 stars
      Buy from Supplier

    86
    New England Biolabs onetaq 2x master mix
    An overview of the MRTK cloning process, parts within the toolkit, origin compatibility, origin copy number and antimicrobial susceptibility for each strain. A) Cloning plasmids (C plasmids) are used as starting material with BsaI restriction enzyme and NEBridge <t>MasterMix</t> to clone the product plasmid (P plasmid). P plasmids can then be transformed into the desired SynCom strain of interest with a time of ∼2.5 hours from plasmid to plate. B) Overview of all 45 parts included in the MRTK and their 4 bp golden gate overhang sequences. C) A table showing the compatibility and copy number of the origins of the MRTK for each strain as well as their antimicrobial susceptibility. Green boxes indicate that the origin is replicated in the strain and that the strain is susceptible to the antimicrobial agent. Red boxes indicate that the origin is not replicated in the strain and that the strain will grow on that antimicrobial agent.
    Onetaq 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq 2x master mix/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq 2x master mix - by Bioz Stars, 2023-09
    86/100 stars
      Buy from Supplier

    86
    Biotechnical Services Inc onetaq 2x master mix
    An overview of the MRTK cloning process, parts within the toolkit, origin compatibility, origin copy number and antimicrobial susceptibility for each strain. A) Cloning plasmids (C plasmids) are used as starting material with BsaI restriction enzyme and NEBridge <t>MasterMix</t> to clone the product plasmid (P plasmid). P plasmids can then be transformed into the desired SynCom strain of interest with a time of ∼2.5 hours from plasmid to plate. B) Overview of all 45 parts included in the MRTK and their 4 bp golden gate overhang sequences. C) A table showing the compatibility and copy number of the origins of the MRTK for each strain as well as their antimicrobial susceptibility. Green boxes indicate that the origin is replicated in the strain and that the strain is susceptible to the antimicrobial agent. Red boxes indicate that the origin is not replicated in the strain and that the strain will grow on that antimicrobial agent.
    Onetaq 2x Master Mix, supplied by Biotechnical Services Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq 2x master mix/product/Biotechnical Services Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq 2x master mix - by Bioz Stars, 2023-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    An overview of the MRTK cloning process, parts within the toolkit, origin compatibility, origin copy number and antimicrobial susceptibility for each strain. A) Cloning plasmids (C plasmids) are used as starting material with BsaI restriction enzyme and NEBridge MasterMix to clone the product plasmid (P plasmid). P plasmids can then be transformed into the desired SynCom strain of interest with a time of ∼2.5 hours from plasmid to plate. B) Overview of all 45 parts included in the MRTK and their 4 bp golden gate overhang sequences. C) A table showing the compatibility and copy number of the origins of the MRTK for each strain as well as their antimicrobial susceptibility. Green boxes indicate that the origin is replicated in the strain and that the strain is susceptible to the antimicrobial agent. Red boxes indicate that the origin is not replicated in the strain and that the strain will grow on that antimicrobial agent.

    Journal: bioRxiv

    Article Title: Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and Identification of Potential Bacterial Chassis for studying Plant-Microbe Interactions

    doi: 10.1101/2023.06.05.543168

    Figure Lengend Snippet: An overview of the MRTK cloning process, parts within the toolkit, origin compatibility, origin copy number and antimicrobial susceptibility for each strain. A) Cloning plasmids (C plasmids) are used as starting material with BsaI restriction enzyme and NEBridge MasterMix to clone the product plasmid (P plasmid). P plasmids can then be transformed into the desired SynCom strain of interest with a time of ∼2.5 hours from plasmid to plate. B) Overview of all 45 parts included in the MRTK and their 4 bp golden gate overhang sequences. C) A table showing the compatibility and copy number of the origins of the MRTK for each strain as well as their antimicrobial susceptibility. Green boxes indicate that the origin is replicated in the strain and that the strain is susceptible to the antimicrobial agent. Red boxes indicate that the origin is not replicated in the strain and that the strain will grow on that antimicrobial agent.

    Article Snippet: Colony PCR reactions for sequence verification were performed with NEB OneTaq HotStart 2x MasterMix (M0484S) and PCR reactions for molecular cloning were performed with Q5 HotStart 2x MasterMix (M0494S).

    Techniques: Clone Assay, Plasmid Preparation, Transformation Assay