oligos New England Biolabs Search Results


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  • 95
    Integrated DNA Technologies oligos
    Oligos, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 95/100, based on 1104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    New England Biolabs nebnext singleplex
    Nebnext Singleplex, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Addgene inc oligos
    Oligos, supplied by Addgene inc, used in various techniques. Bioz Stars score: 99/100, based on 1506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    New England Biolabs multiplex oligos
    Multiplex Oligos, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    New England Biolabs multiplex oligos kit
    Multiplex Oligos Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    New England Biolabs nebnext mutiplex oligos
    Nebnext Mutiplex Oligos, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    New England Biolabs dna oligonucleotides
    Dna Oligonucleotides, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    New England Biolabs random nonamer oligonucleotides
    Random Nonamer Oligonucleotides, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 83/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs e7335l index primers
    E7335l Index Primers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs nebnext multiplex oligos
    Nebnext Multiplex Oligos, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 3803 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs nebnext multiple oligos
    Nebnext Multiple Oligos, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    New England Biolabs sequence octamer oligonucleotides
    Sequence Octamer Oligonucleotides, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    75
    New England Biolabs akt sirna oligos
    Requirement for <t>Akt</t> in TNF-α production by T cells from multiple sources. A and B , comparison of Akt i 1/2 and Akt <t>siRNA</t> effects on TNF-α production. The murine Th2 cell clone D10 ( A ) or bulk murine Th1 cells ( B ) were stimulated as indicated,
    Akt Sirna Oligos, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs 18 mer oligo dt
    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled <t>18-mer</t> <t>oligo</t> dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.
    18 Mer Oligo Dt, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    New England Biolabs tttttt terminator sequences oligos
    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled <t>18-mer</t> <t>oligo</t> dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.
    Tttttt Terminator Sequences Oligos, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    New England Biolabs 25 nt unphosphorylated ssrna oligonucleotides
    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled <t>18-mer</t> <t>oligo</t> dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.
    25 Nt Unphosphorylated Ssrna Oligonucleotides, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    New England Biolabs dual index nebnext multiplex oligos
    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled <t>18-mer</t> <t>oligo</t> dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.
    Dual Index Nebnext Multiplex Oligos, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Beckman Coulter nebnext multiplex oligos for illumina index primers set 2
    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled <t>18-mer</t> <t>oligo</t> dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.
    Nebnext Multiplex Oligos For Illumina Index Primers Set 2, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    New England Biolabs n m akt shortcut sirna oligos
    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled <t>18-mer</t> <t>oligo</t> dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.
    N M Akt Shortcut Sirna Oligos, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    75
    New England Biolabs oligonucleotides bst dna polymerase large fragment
    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled <t>18-mer</t> <t>oligo</t> dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.
    Oligonucleotides Bst Dna Polymerase Large Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    New England Biolabs oligo d t 23vn
    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled <t>18-mer</t> <t>oligo</t> dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.
    Oligo D T 23vn, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs oligo dt25 magnetic beads
    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled <t>18-mer</t> <t>oligo</t> dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.
    Oligo Dt25 Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs oligo
    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled <t>18-mer</t> <t>oligo</t> dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.
    Oligo, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs nebnext multiplex oligos for illumina methylated adaptor index primers set 1
    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled <t>18-mer</t> <t>oligo</t> dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.
    Nebnext Multiplex Oligos For Illumina Methylated Adaptor Index Primers Set 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs t7 endonuclease i
    Characterization of the elongation products produced. ( A )  Different forms of products of oligonucleotide elongation . Form  1 . Tpg (large filled circle) linked to 1–3 radioactive dCMP (small filled circle). Form  2 . Tpg linked to radioactive dCMP and non-radioactive dNMP (open filled circle) with the total length not exceeding 7 nt. Form  3 . Tpg linked to13 nt-oligomer (Palindrome I) complexed with the template strand (shaded circles), the length of which is not to scale. Form  4 . Tpg attached to the 13-nt oligonucleotide (which fold back to form a hairpin) without the template, representing the denaturation product of Form  3 . Form  5 . Tpg linked to 13-nt oligomer duplexed with the template strand of 14–16 nt, representing T7 endonuclease I digestion product of Form  3 . Form  1 ′– 5 ′ proteinase K digestion products of Form  1 – 5 . Tpg was removed from the oligonucleotides. ( B )  The products of the first step of end patching based on TO65 . The template was 65- (left and middle panels) or 89-nt (right panel) telomere DNA. The reaction conditions were as in Figure   2B  except that the product was eluted from the G-25 spin column in deionized water. The eluted product was subjected to proteinase K (‘PK’) digestion, Exo III (‘EX’) digestion, or heat denaturation (‘Δ’).  Left panel . Only radioactive dCTP (‘dC’) was included.  Middle panel . Radioactive dCTP and non-radioactive dATP, dTTP and dGTP (‘all dN’) were included. The different forms of products identified in the SDS gels were marked. The sizes of marker proteins (in kD) are indicated to the right. ( C )  The products produced on templates of different lengths. Upper  panel. The reactions were carried under the same conditions as described in (B) with all dNTPs added. The lengths of the template strands used are as indicated. In three cases, the incubation time was increased from 10 m to 30 and 60 m as indicated. Some products were further treated with T7 endonuclease I (‘Endo’).  Lower panel . The products were heat-denatured before being subjected to electrophoresis. The various forms of the products are marked in the gels. The product, which was eluted for further analysis in (D) is marked by an asterisk. ( D )  Sizing the length of the oligonucleotide . Form  3  produced from the 89 nt template (marked with an asterisk in (C) was eluted from the gel slice in 0.1% SDS by soaking. The eluent was concentrated by ethanol precipitation, re-dissolved in 1 N NaOH, and incubated at 37°C for 2 h. After neutralization with HCl, the sample was electrophoresed in a 15% urea–PAGE at 20 V/cm together with three size markers—single-stranded DNA of TO13, TO16 and TO35 as indicated. The positions of the size markers were determined by UV absorption and EtBr staining. The position of the products was determined by autoradiography.
    T7 Endonuclease I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2927 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Requirement for Akt in TNF-α production by T cells from multiple sources. A and B , comparison of Akt i 1/2 and Akt siRNA effects on TNF-α production. The murine Th2 cell clone D10 ( A ) or bulk murine Th1 cells ( B ) were stimulated as indicated,

    Journal: The Journal of Biological Chemistry

    Article Title: Akt Fine-tunes NF-\u03baB-dependent Gene Expression during T Cell Activation

    doi: 10.1074/jbc.M111.259549

    Figure Lengend Snippet: Requirement for Akt in TNF-α production by T cells from multiple sources. A and B , comparison of Akt i 1/2 and Akt siRNA effects on TNF-α production. The murine Th2 cell clone D10 ( A ) or bulk murine Th1 cells ( B ) were stimulated as indicated,

    Article Snippet: Akt siRNA oligos were from New England Biolabs.

    Techniques:

    mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled 18-mer oligo dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.

    Journal: PLoS Genetics

    Article Title: MOS11: A New Component in the mRNA Export Pathway

    doi: 10.1371/journal.pgen.1001250

    Figure Lengend Snippet: mRNA export is impaired in mos11 plants. (A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2 . The same amount of WT, mos11-1 , and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by 32 P-ATP labeled 18-mer oligo dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.

    Article Snippet: The 18-mer oligo-dT was labeled with 32 P by the T4 poly-nucleotide kinase (NEB, Cat. No. M0201) and added to the pre-hybridization buffer.

    Techniques: Dot Blot, Labeling, In Situ, Microscopy

    Characterization of the elongation products produced. ( A )  Different forms of products of oligonucleotide elongation . Form  1 . Tpg (large filled circle) linked to 1–3 radioactive dCMP (small filled circle). Form  2 . Tpg linked to radioactive dCMP and non-radioactive dNMP (open filled circle) with the total length not exceeding 7 nt. Form  3 . Tpg linked to13 nt-oligomer (Palindrome I) complexed with the template strand (shaded circles), the length of which is not to scale. Form  4 . Tpg attached to the 13-nt oligonucleotide (which fold back to form a hairpin) without the template, representing the denaturation product of Form  3 . Form  5 . Tpg linked to 13-nt oligomer duplexed with the template strand of 14–16 nt, representing T7 endonuclease I digestion product of Form  3 . Form  1 ′– 5 ′ proteinase K digestion products of Form  1 – 5 . Tpg was removed from the oligonucleotides. ( B )  The products of the first step of end patching based on TO65 . The template was 65- (left and middle panels) or 89-nt (right panel) telomere DNA. The reaction conditions were as in Figure   2B  except that the product was eluted from the G-25 spin column in deionized water. The eluted product was subjected to proteinase K (‘PK’) digestion, Exo III (‘EX’) digestion, or heat denaturation (‘Δ’).  Left panel . Only radioactive dCTP (‘dC’) was included.  Middle panel . Radioactive dCTP and non-radioactive dATP, dTTP and dGTP (‘all dN’) were included. The different forms of products identified in the SDS gels were marked. The sizes of marker proteins (in kD) are indicated to the right. ( C )  The products produced on templates of different lengths. Upper  panel. The reactions were carried under the same conditions as described in (B) with all dNTPs added. The lengths of the template strands used are as indicated. In three cases, the incubation time was increased from 10 m to 30 and 60 m as indicated. Some products were further treated with T7 endonuclease I (‘Endo’).  Lower panel . The products were heat-denatured before being subjected to electrophoresis. The various forms of the products are marked in the gels. The product, which was eluted for further analysis in (D) is marked by an asterisk. ( D )  Sizing the length of the oligonucleotide . Form  3  produced from the 89 nt template (marked with an asterisk in (C) was eluted from the gel slice in 0.1% SDS by soaking. The eluent was concentrated by ethanol precipitation, re-dissolved in 1 N NaOH, and incubated at 37°C for 2 h. After neutralization with HCl, the sample was electrophoresed in a 15% urea–PAGE at 20 V/cm together with three size markers—single-stranded DNA of TO13, TO16 and TO35 as indicated. The positions of the size markers were determined by UV absorption and EtBr staining. The position of the products was determined by autoradiography.

    Journal: Nucleic Acids Research

    Article Title: Telomere-associated proteins add deoxynucleotides to terminal proteins during replication of the telomeres of linear chromosomes and plasmids in Streptomyces

    doi: 10.1093/nar/gkv302

    Figure Lengend Snippet: Characterization of the elongation products produced. ( A ) Different forms of products of oligonucleotide elongation . Form 1 . Tpg (large filled circle) linked to 1–3 radioactive dCMP (small filled circle). Form 2 . Tpg linked to radioactive dCMP and non-radioactive dNMP (open filled circle) with the total length not exceeding 7 nt. Form 3 . Tpg linked to13 nt-oligomer (Palindrome I) complexed with the template strand (shaded circles), the length of which is not to scale. Form 4 . Tpg attached to the 13-nt oligonucleotide (which fold back to form a hairpin) without the template, representing the denaturation product of Form 3 . Form 5 . Tpg linked to 13-nt oligomer duplexed with the template strand of 14–16 nt, representing T7 endonuclease I digestion product of Form 3 . Form 1 ′– 5 ′ proteinase K digestion products of Form 1 – 5 . Tpg was removed from the oligonucleotides. ( B ) The products of the first step of end patching based on TO65 . The template was 65- (left and middle panels) or 89-nt (right panel) telomere DNA. The reaction conditions were as in Figure 2B except that the product was eluted from the G-25 spin column in deionized water. The eluted product was subjected to proteinase K (‘PK’) digestion, Exo III (‘EX’) digestion, or heat denaturation (‘Δ’). Left panel . Only radioactive dCTP (‘dC’) was included. Middle panel . Radioactive dCTP and non-radioactive dATP, dTTP and dGTP (‘all dN’) were included. The different forms of products identified in the SDS gels were marked. The sizes of marker proteins (in kD) are indicated to the right. ( C ) The products produced on templates of different lengths. Upper panel. The reactions were carried under the same conditions as described in (B) with all dNTPs added. The lengths of the template strands used are as indicated. In three cases, the incubation time was increased from 10 m to 30 and 60 m as indicated. Some products were further treated with T7 endonuclease I (‘Endo’). Lower panel . The products were heat-denatured before being subjected to electrophoresis. The various forms of the products are marked in the gels. The product, which was eluted for further analysis in (D) is marked by an asterisk. ( D ) Sizing the length of the oligonucleotide . Form 3 produced from the 89 nt template (marked with an asterisk in (C) was eluted from the gel slice in 0.1% SDS by soaking. The eluent was concentrated by ethanol precipitation, re-dissolved in 1 N NaOH, and incubated at 37°C for 2 h. After neutralization with HCl, the sample was electrophoresed in a 15% urea–PAGE at 20 V/cm together with three size markers—single-stranded DNA of TO13, TO16 and TO35 as indicated. The positions of the size markers were determined by UV absorption and EtBr staining. The position of the products was determined by autoradiography.

    Article Snippet: This suggested that the oligonucleotide synthesis did not extend beyond the junction between Palindromes I and II, leaving it exposed to T7 endonuclease I.

    Techniques: Produced, Marker, Incubation, Electrophoresis, Ethanol Precipitation, Neutralization, Polyacrylamide Gel Electrophoresis, Staining, Autoradiography