oligos Agilent Technologies Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 79
    Agilent technologies oligonucleotides oligos
    Oligonucleotides Oligos, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligonucleotides oligos/product/Agilent technologies
    Average 79 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    oligonucleotides oligos - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    78
    Agilent technologies oligonucleotide microarray technology agilent oligonucleotide technology
    <t>Microarray</t> testing results. The CNVs of Cases 1, 3, 6, and 7 were detected by aCGH. (a)–(d) showed aCGH results of Cases 1, 3, 6, and 7, respectively. (a) A 3.44-Mb deletion within chromosome 7q in Case 1. (b) A 3.25-Mb deletion in chromosome 2q in Case 3 harbored four OMIM genes. (c) A 4.03-Mb deletion of chromosome 19p in Case 6. (d) A 2.79-Mb deletion in chromosome 4q35.2 in Case 7. The respective chromosomes are shown and labeled. Signal intensity is plotted on a log⁡ 2 ⁡ scale, such that a normal copy number gives a value of 0. Chromosomal deletions are denoted by leftward deviation of the central line (marked by red boxes).
    Oligonucleotide Microarray Technology Agilent Oligonucleotide Technology, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligonucleotide microarray technology agilent oligonucleotide technology/product/Agilent technologies
    Average 78 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    oligonucleotide microarray technology agilent oligonucleotide technology - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    79
    Agilent technologies 60mer oligonucleotides
    <t>Microarray</t> testing results. The CNVs of Cases 1, 3, 6, and 7 were detected by aCGH. (a)–(d) showed aCGH results of Cases 1, 3, 6, and 7, respectively. (a) A 3.44-Mb deletion within chromosome 7q in Case 1. (b) A 3.25-Mb deletion in chromosome 2q in Case 3 harbored four OMIM genes. (c) A 4.03-Mb deletion of chromosome 19p in Case 6. (d) A 2.79-Mb deletion in chromosome 4q35.2 in Case 7. The respective chromosomes are shown and labeled. Signal intensity is plotted on a log⁡ 2 ⁡ scale, such that a normal copy number gives a value of 0. Chromosomal deletions are denoted by leftward deviation of the central line (marked by red boxes).
    60mer Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/60mer oligonucleotides/product/Agilent technologies
    Average 79 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    60mer oligonucleotides - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    80
    Agilent technologies bp oligos
    <t>Microarray</t> testing results. The CNVs of Cases 1, 3, 6, and 7 were detected by aCGH. (a)–(d) showed aCGH results of Cases 1, 3, 6, and 7, respectively. (a) A 3.44-Mb deletion within chromosome 7q in Case 1. (b) A 3.25-Mb deletion in chromosome 2q in Case 3 harbored four OMIM genes. (c) A 4.03-Mb deletion of chromosome 19p in Case 6. (d) A 2.79-Mb deletion in chromosome 4q35.2 in Case 7. The respective chromosomes are shown and labeled. Signal intensity is plotted on a log⁡ 2 ⁡ scale, such that a normal copy number gives a value of 0. Chromosomal deletions are denoted by leftward deviation of the central line (marked by red boxes).
    Bp Oligos, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp oligos/product/Agilent technologies
    Average 80 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    bp oligos - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    78
    Agilent technologies format oligo arrays 014868 agilent technologies
    Global gene expression is altered by G3134T hGRβ polymorphism. (a) mRNA isolated from three biological replicates of each genotype were analyzed by the <t>Agilent</t> Whole Human Genome 4 × 44 multiplex format <t>oligo</t> array for gene expression. Principal component analysis was performed in Partek Genomics Suite to visualize the variance within each genotype. The number of probes statistically different compared to U-OFF cells at P
    Format Oligo Arrays 014868 Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/format oligo arrays 014868 agilent technologies/product/Agilent technologies
    Average 78 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    format oligo arrays 014868 agilent technologies - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    77
    Agilent technologies 51 mer oligonucleotides
    Global gene expression is altered by G3134T hGRβ polymorphism. (a) mRNA isolated from three biological replicates of each genotype were analyzed by the <t>Agilent</t> Whole Human Genome 4 × 44 multiplex format <t>oligo</t> array for gene expression. Principal component analysis was performed in Partek Genomics Suite to visualize the variance within each genotype. The number of probes statistically different compared to U-OFF cells at P
    51 Mer Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/51 mer oligonucleotides/product/Agilent technologies
    Average 77 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    51 mer oligonucleotides - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    85
    Agilent technologies 60 mer oligonucleotides probes
    Global gene expression is altered by G3134T hGRβ polymorphism. (a) mRNA isolated from three biological replicates of each genotype were analyzed by the <t>Agilent</t> Whole Human Genome 4 × 44 multiplex format <t>oligo</t> array for gene expression. Principal component analysis was performed in Partek Genomics Suite to visualize the variance within each genotype. The number of probes statistically different compared to U-OFF cells at P
    60 Mer Oligonucleotides Probes, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/60 mer oligonucleotides probes/product/Agilent technologies
    Average 85 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    60 mer oligonucleotides probes - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    79
    Agilent technologies 9300 oligos
    Global gene expression is altered by G3134T hGRβ polymorphism. (a) mRNA isolated from three biological replicates of each genotype were analyzed by the <t>Agilent</t> Whole Human Genome 4 × 44 multiplex format <t>oligo</t> array for gene expression. Principal component analysis was performed in Partek Genomics Suite to visualize the variance within each genotype. The number of probes statistically different compared to U-OFF cells at P
    9300 Oligos, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/9300 oligos/product/Agilent technologies
    Average 79 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    9300 oligos - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    79
    Agilent technologies barcoded oligonucleotides
    Global gene expression is altered by G3134T hGRβ polymorphism. (a) mRNA isolated from three biological replicates of each genotype were analyzed by the <t>Agilent</t> Whole Human Genome 4 × 44 multiplex format <t>oligo</t> array for gene expression. Principal component analysis was performed in Partek Genomics Suite to visualize the variance within each genotype. The number of probes statistically different compared to U-OFF cells at P
    Barcoded Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/barcoded oligonucleotides/product/Agilent technologies
    Average 79 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    barcoded oligonucleotides - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    90
    Twist Bioscience mcp oligos
    Global gene expression is altered by G3134T hGRβ polymorphism. (a) mRNA isolated from three biological replicates of each genotype were analyzed by the <t>Agilent</t> Whole Human Genome 4 × 44 multiplex format <t>oligo</t> array for gene expression. Principal component analysis was performed in Partek Genomics Suite to visualize the variance within each genotype. The number of probes statistically different compared to U-OFF cells at P
    Mcp Oligos, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mcp oligos/product/Twist Bioscience
    Average 90 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    mcp oligos - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    83
    Agilent technologies microarray oligos
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Microarray Oligos, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 83/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray oligos/product/Agilent technologies
    Average 83 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    microarray oligos - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    79
    Agilent technologies barcoding oligos
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Barcoding Oligos, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/barcoding oligos/product/Agilent technologies
    Average 79 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    barcoding oligos - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    77
    Agilent technologies mutagenic oligonucleotides
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Mutagenic Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mutagenic oligonucleotides/product/Agilent technologies
    Average 77 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    mutagenic oligonucleotides - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    77
    Agilent technologies custom built oligonucleotide agilent technologies 4x44k microarrays
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Custom Built Oligonucleotide Agilent Technologies 4x44k Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/custom built oligonucleotide agilent technologies 4x44k microarrays/product/Agilent technologies
    Average 77 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    custom built oligonucleotide agilent technologies 4x44k microarrays - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    78
    Agilent technologies array synthesized 230 bp oligos
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Array Synthesized 230 Bp Oligos, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/array synthesized 230 bp oligos/product/Agilent technologies
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    array synthesized 230 bp oligos - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    80
    Agilent technologies 150bp ssdna oligos
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    150bp Ssdna Oligos, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/150bp ssdna oligos/product/Agilent technologies
    Average 80 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    150bp ssdna oligos - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    80
    Agilent technologies native agilent oligos
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Native Agilent Oligos, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/native agilent oligos/product/Agilent technologies
    Average 80 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    native agilent oligos - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    77
    Agilent technologies agilent advancebio oligonucleotides
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Agilent Advancebio Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent advancebio oligonucleotides/product/Agilent technologies
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    agilent advancebio oligonucleotides - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    90
    Agilent technologies sureselect adapter oligos
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Sureselect Adapter Oligos, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sureselect adapter oligos/product/Agilent technologies
    Average 90 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    sureselect adapter oligos - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    80
    Agilent technologies 60 mer oligomicroarray canine oligo microarray kit agilent technologies
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    60 Mer Oligomicroarray Canine Oligo Microarray Kit Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/60 mer oligomicroarray canine oligo microarray kit agilent technologies/product/Agilent technologies
    Average 80 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    60 mer oligomicroarray canine oligo microarray kit agilent technologies - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    76
    Agilent technologies crna oligonucleotides
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Crna Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 76/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/crna oligonucleotides/product/Agilent technologies
    Average 76 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    crna oligonucleotides - by Bioz Stars, 2020-02
    76/100 stars
      Buy from Supplier

    77
    Agilent technologies custom oligonucleotides
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Custom Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/custom oligonucleotides/product/Agilent technologies
    Average 77 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    custom oligonucleotides - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    79
    Agilent technologies mirna microarray analysis agilent oligonucleotide technology
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Mirna Microarray Analysis Agilent Oligonucleotide Technology, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirna microarray analysis agilent oligonucleotide technology/product/Agilent technologies
    Average 79 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mirna microarray analysis agilent oligonucleotide technology - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    77
    Agilent technologies preparation synthetic 140 bp oligos
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Preparation Synthetic 140 Bp Oligos, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preparation synthetic 140 bp oligos/product/Agilent technologies
    Average 77 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    preparation synthetic 140 bp oligos - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    79
    Agilent technologies microchip oligonucleotides lyophilized agilent microchip oligonucleotides
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Microchip Oligonucleotides Lyophilized Agilent Microchip Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microchip oligonucleotides lyophilized agilent microchip oligonucleotides/product/Agilent technologies
    Average 79 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    microchip oligonucleotides lyophilized agilent microchip oligonucleotides - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    79
    Agilent technologies biotinylated custom bait oligonucleotides
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Biotinylated Custom Bait Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated custom bait oligonucleotides/product/Agilent technologies
    Average 79 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    biotinylated custom bait oligonucleotides - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    89
    Agilent technologies unique 200 mer oligonucleotides
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Unique 200 Mer Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unique 200 mer oligonucleotides/product/Agilent technologies
    Average 89 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    unique 200 mer oligonucleotides - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    84
    Agilent technologies agilent paired end oligonucleotides
    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of <t>microarray-synthesized</t> <t>oligos,</t> was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.
    Agilent Paired End Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent paired end oligonucleotides/product/Agilent technologies
    Average 84 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    agilent paired end oligonucleotides - by Bioz Stars, 2020-02
    84/100 stars
      Buy from Supplier

    78
    Agilent technologies positive control oligos
    Senegalese sole microarray platform . Scatter plot of the signal intensities in the Cy3 and Cy5 channel of replica analysis of self-to-self (A) and differential gene expression (C) experiments. In both panels, the green and blue lines parallel to the black diagonal line represent the ± 3σ limits of the data from control and S. <t>senegalensis</t> specific <t>oligos,</t> respectively. The histograms of the distribution of fold changes (FC) as log2(S/C) for control and S. senegalensis specific oligos in the self-to-self (B) and differential gene expression (D) experiments are also shown. In these panels, the green and blue curves represent the ± 3σ limits on the data from control and S. senegalensis specific oligos, respectively, and the red dotted lines in the self-to-self histogram represent the cut-off chosen in the differential gene expression experiment to estimate overall FDR.
    Positive Control Oligos, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/positive control oligos/product/Agilent technologies
    Average 78 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    positive control oligos - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    77
    Agilent technologies sureselect xt capture oligonucleotides
    Senegalese sole microarray platform . Scatter plot of the signal intensities in the Cy3 and Cy5 channel of replica analysis of self-to-self (A) and differential gene expression (C) experiments. In both panels, the green and blue lines parallel to the black diagonal line represent the ± 3σ limits of the data from control and S. <t>senegalensis</t> specific <t>oligos,</t> respectively. The histograms of the distribution of fold changes (FC) as log2(S/C) for control and S. senegalensis specific oligos in the self-to-self (B) and differential gene expression (D) experiments are also shown. In these panels, the green and blue curves represent the ± 3σ limits on the data from control and S. senegalensis specific oligos, respectively, and the red dotted lines in the self-to-self histogram represent the cut-off chosen in the differential gene expression experiment to estimate overall FDR.
    Sureselect Xt Capture Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sureselect xt capture oligonucleotides/product/Agilent technologies
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    sureselect xt capture oligonucleotides - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    77
    Agilent technologies custom 60mer oligonucleotides
    Senegalese sole microarray platform . Scatter plot of the signal intensities in the Cy3 and Cy5 channel of replica analysis of self-to-self (A) and differential gene expression (C) experiments. In both panels, the green and blue lines parallel to the black diagonal line represent the ± 3σ limits of the data from control and S. <t>senegalensis</t> specific <t>oligos,</t> respectively. The histograms of the distribution of fold changes (FC) as log2(S/C) for control and S. senegalensis specific oligos in the self-to-self (B) and differential gene expression (D) experiments are also shown. In these panels, the green and blue curves represent the ± 3σ limits on the data from control and S. senegalensis specific oligos, respectively, and the red dotted lines in the self-to-self histogram represent the cut-off chosen in the differential gene expression experiment to estimate overall FDR.
    Custom 60mer Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/custom 60mer oligonucleotides/product/Agilent technologies
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    custom 60mer oligonucleotides - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    77
    Agilent technologies eber 2 oligonucleotides
    Senegalese sole microarray platform . Scatter plot of the signal intensities in the Cy3 and Cy5 channel of replica analysis of self-to-self (A) and differential gene expression (C) experiments. In both panels, the green and blue lines parallel to the black diagonal line represent the ± 3σ limits of the data from control and S. <t>senegalensis</t> specific <t>oligos,</t> respectively. The histograms of the distribution of fold changes (FC) as log2(S/C) for control and S. senegalensis specific oligos in the self-to-self (B) and differential gene expression (D) experiments are also shown. In these panels, the green and blue curves represent the ± 3σ limits on the data from control and S. senegalensis specific oligos, respectively, and the red dotted lines in the self-to-self histogram represent the cut-off chosen in the differential gene expression experiment to estimate overall FDR.
    Eber 2 Oligonucleotides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eber 2 oligonucleotides/product/Agilent technologies
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    eber 2 oligonucleotides - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    Image Search Results


    Microarray testing results. The CNVs of Cases 1, 3, 6, and 7 were detected by aCGH. (a)–(d) showed aCGH results of Cases 1, 3, 6, and 7, respectively. (a) A 3.44-Mb deletion within chromosome 7q in Case 1. (b) A 3.25-Mb deletion in chromosome 2q in Case 3 harbored four OMIM genes. (c) A 4.03-Mb deletion of chromosome 19p in Case 6. (d) A 2.79-Mb deletion in chromosome 4q35.2 in Case 7. The respective chromosomes are shown and labeled. Signal intensity is plotted on a log⁡ 2 ⁡ scale, such that a normal copy number gives a value of 0. Chromosomal deletions are denoted by leftward deviation of the central line (marked by red boxes).

    Journal: BioMed Research International

    Article Title: Prenatal Diagnosis of Central Nervous System Anomalies by High-Resolution Chromosomal Microarray Analysis

    doi: 10.1155/2015/426379

    Figure Lengend Snippet: Microarray testing results. The CNVs of Cases 1, 3, 6, and 7 were detected by aCGH. (a)–(d) showed aCGH results of Cases 1, 3, 6, and 7, respectively. (a) A 3.44-Mb deletion within chromosome 7q in Case 1. (b) A 3.25-Mb deletion in chromosome 2q in Case 3 harbored four OMIM genes. (c) A 4.03-Mb deletion of chromosome 19p in Case 6. (d) A 2.79-Mb deletion in chromosome 4q35.2 in Case 7. The respective chromosomes are shown and labeled. Signal intensity is plotted on a log⁡ 2 ⁡ scale, such that a normal copy number gives a value of 0. Chromosomal deletions are denoted by leftward deviation of the central line (marked by red boxes).

    Article Snippet: Genomic DNA was extracted from 2 mL of umbilical cord blood with a commercially available Blood Genomic DNA Extraction kit (BioChain Institute Inc., Newark, CA or Yuanpinghao Biotech Co., Ltd., resp.) according to the manufacturer's instructions. aCGH was performed using 8 × 60 K oligonucleotide-based microarray (Agilent), and SNP array was detected using a 750 K microarray (Affymetrix CytoScan 750 K Array).

    Techniques: Microarray, Labeling

    Microarray testing results. The CNVs of Cases 2, 4, 5, and 8 were detected by SNP array. (a)–(d) showed the SNP results of Cases 2, 4, 5, and 8, respectively. (a) A 3.15-Mb duplication in chromosome 22q11.21 included 43 OMIM genes in Case 2. (b) A 0.17-Mb deletion in chromosome Xq13.3 in Case 4. (c) A 0.34-Mb deletion within chromosome 2p21 in Case 5. (d) A 1.15-Mb deletion of chromosome 21q21.1 in Case 8. The chromosome numbers and cytobands are shown and labeled on the right side. The view on the left side shows the detected segments, regions, and reference annotations in detail. Chromosomal duplication segments are denoted by upward triangle (blue) whereas deletion segments are denoted by downward triangle (red).

    Journal: BioMed Research International

    Article Title: Prenatal Diagnosis of Central Nervous System Anomalies by High-Resolution Chromosomal Microarray Analysis

    doi: 10.1155/2015/426379

    Figure Lengend Snippet: Microarray testing results. The CNVs of Cases 2, 4, 5, and 8 were detected by SNP array. (a)–(d) showed the SNP results of Cases 2, 4, 5, and 8, respectively. (a) A 3.15-Mb duplication in chromosome 22q11.21 included 43 OMIM genes in Case 2. (b) A 0.17-Mb deletion in chromosome Xq13.3 in Case 4. (c) A 0.34-Mb deletion within chromosome 2p21 in Case 5. (d) A 1.15-Mb deletion of chromosome 21q21.1 in Case 8. The chromosome numbers and cytobands are shown and labeled on the right side. The view on the left side shows the detected segments, regions, and reference annotations in detail. Chromosomal duplication segments are denoted by upward triangle (blue) whereas deletion segments are denoted by downward triangle (red).

    Article Snippet: Genomic DNA was extracted from 2 mL of umbilical cord blood with a commercially available Blood Genomic DNA Extraction kit (BioChain Institute Inc., Newark, CA or Yuanpinghao Biotech Co., Ltd., resp.) according to the manufacturer's instructions. aCGH was performed using 8 × 60 K oligonucleotide-based microarray (Agilent), and SNP array was detected using a 750 K microarray (Affymetrix CytoScan 750 K Array).

    Techniques: Microarray, Labeling

    Global gene expression is altered by G3134T hGRβ polymorphism. (a) mRNA isolated from three biological replicates of each genotype were analyzed by the Agilent Whole Human Genome 4 × 44 multiplex format oligo array for gene expression. Principal component analysis was performed in Partek Genomics Suite to visualize the variance within each genotype. The number of probes statistically different compared to U-OFF cells at P

    Journal: Laboratory investigation; a journal of technical methods and pathology

    Article Title: Generating diversity in human glucocorticoid signaling through a racially diverse polymorphism in the beta isoform of the glucocorticoid receptor

    doi: 10.1038/labinvest.2017.76

    Figure Lengend Snippet: Global gene expression is altered by G3134T hGRβ polymorphism. (a) mRNA isolated from three biological replicates of each genotype were analyzed by the Agilent Whole Human Genome 4 × 44 multiplex format oligo array for gene expression. Principal component analysis was performed in Partek Genomics Suite to visualize the variance within each genotype. The number of probes statistically different compared to U-OFF cells at P

    Article Snippet: Gene expression analysis was conducted using Agilent Whole Human Genome 4×44 multiplex format oligo arrays (014850; Agilent Technologies) following the Agilent 1-color microarray-based gene expression analysis protocol.

    Techniques: Expressing, Isolation, Multiplex Assay

    Glucocorticoid-regulated gene expression is altered by the presence of the G3134T hGRβ polymorphism in human macrophages. (a) mRNA isolated from seven WT hGRβ participants and three homozygous G3134T hGRβ participants were analyzed by the Agilent Whole Human Genome 4 × 44 multiplex format oligo array for gene expression. Heat map of significantly regulated gene probes by genotype was created with Partek Genomics Suite software and visually represent unique and commonly regulated targets. Red indicates induced genes and green indicates repressed genes. (b) The 25 top Canonical Signaling Pathways were visualized and shared functions identified by lines connecting nodes. The intensity of the red color is proportional to significance of P -value as determined by the Fisher’s Exact Test. (c) The number of probes statistically different following 6 h treatment with 100 nM dexamethasone compared to vehicle at P

    Journal: Laboratory investigation; a journal of technical methods and pathology

    Article Title: Generating diversity in human glucocorticoid signaling through a racially diverse polymorphism in the beta isoform of the glucocorticoid receptor

    doi: 10.1038/labinvest.2017.76

    Figure Lengend Snippet: Glucocorticoid-regulated gene expression is altered by the presence of the G3134T hGRβ polymorphism in human macrophages. (a) mRNA isolated from seven WT hGRβ participants and three homozygous G3134T hGRβ participants were analyzed by the Agilent Whole Human Genome 4 × 44 multiplex format oligo array for gene expression. Heat map of significantly regulated gene probes by genotype was created with Partek Genomics Suite software and visually represent unique and commonly regulated targets. Red indicates induced genes and green indicates repressed genes. (b) The 25 top Canonical Signaling Pathways were visualized and shared functions identified by lines connecting nodes. The intensity of the red color is proportional to significance of P -value as determined by the Fisher’s Exact Test. (c) The number of probes statistically different following 6 h treatment with 100 nM dexamethasone compared to vehicle at P

    Article Snippet: Gene expression analysis was conducted using Agilent Whole Human Genome 4×44 multiplex format oligo arrays (014850; Agilent Technologies) following the Agilent 1-color microarray-based gene expression analysis protocol.

    Techniques: Expressing, Isolation, Multiplex Assay, Software

    Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of microarray-synthesized oligos, was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.

    Journal: Nucleic Acids Research

    Article Title: High-quality gene assembly directly from unpurified mixtures of microarray-synthesized oligonucleotides

    doi: 10.1093/nar/gkq677

    Figure Lengend Snippet: Optimizing assembly of blocks into template for gene production. ( A ) Evaluating effect of annealing time. A 252-element mixture of Mly I cleaved blocks (20 fmol/block) assembled from a 2000-complex pool of microarray-synthesized oligos, was subjected to five cycles of PCA with increasing annealing times from 0 to 35 min. To evaluate robustness of the PCA-generated template 1 µl aliquots of the reactions were used for amplification of 2.1 and 1.3 kb genes with pairs of gene-specific primers. The amplified products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Lanes 1–6—products amplified from the templates generated by the reactions that carried, respectively, 0, 2, 9, 18, 28 and 35-min long annealing steps; lane 7—100 bp DNA ladder (NEB); and lane 8–1 kb DNA ladder (NEB). ( B ) Evaluating effect of PCR cycle number. The block mixture described in ( A ) was subjected to an increasing number of PCA cycles performed with a 25-min annealing step. Robustness of the generated templates was evaluated as described in the ( A ). Lane 1—1 kb DNA ladder (NEB); and lanes 2–12—products amplified from the templates assembled using 0 to 10 PCA cycles, respectively.

    Article Snippet: Optimization of PCA conditions was performed on the 252-complex block mixture described above assembled from microarray oligos synthesized by Agilent.

    Techniques: Blocking Assay, Microarray, Synthesized, Generated, Amplification, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction

    Senegalese sole microarray platform . Scatter plot of the signal intensities in the Cy3 and Cy5 channel of replica analysis of self-to-self (A) and differential gene expression (C) experiments. In both panels, the green and blue lines parallel to the black diagonal line represent the ± 3σ limits of the data from control and S. senegalensis specific oligos, respectively. The histograms of the distribution of fold changes (FC) as log2(S/C) for control and S. senegalensis specific oligos in the self-to-self (B) and differential gene expression (D) experiments are also shown. In these panels, the green and blue curves represent the ± 3σ limits on the data from control and S. senegalensis specific oligos, respectively, and the red dotted lines in the self-to-self histogram represent the cut-off chosen in the differential gene expression experiment to estimate overall FDR.

    Journal: BMC Genomics

    Article Title: Genomic resources for a commercial flatfish, the Senegalese sole (Solea senegalensis): EST sequencing, oligo microarray design, and development of the Soleamold bioinformatic platform

    doi: 10.1186/1471-2164-9-508

    Figure Lengend Snippet: Senegalese sole microarray platform . Scatter plot of the signal intensities in the Cy3 and Cy5 channel of replica analysis of self-to-self (A) and differential gene expression (C) experiments. In both panels, the green and blue lines parallel to the black diagonal line represent the ± 3σ limits of the data from control and S. senegalensis specific oligos, respectively. The histograms of the distribution of fold changes (FC) as log2(S/C) for control and S. senegalensis specific oligos in the self-to-self (B) and differential gene expression (D) experiments are also shown. In these panels, the green and blue curves represent the ± 3σ limits on the data from control and S. senegalensis specific oligos, respectively, and the red dotted lines in the self-to-self histogram represent the cut-off chosen in the differential gene expression experiment to estimate overall FDR.

    Article Snippet: The final design of each slide, comprising 2 arrays with gene specific probes for all 5,087 S. senegalensis unigenes as well as negative and positive control oligos, was submitted for synthesis to Agilent using the eArray platform.

    Techniques: Microarray, Expressing